LOCUS       DAOOON010000001        75621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_353_length_75621_cov_20.363894, whole genome shotgun sequence.
ACCESSION   DAOOON010000001 DAOOON010000000
VERSION     DAOOON010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 75621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75621
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_75621_cov_20.363894"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLK55_00005"
     CDS             <1..371
                     /locus_tag="PLK55_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35354.1"
                     /translation="VNTANELVNQKCDKATIQITDCVINDDGNVVLTIKNTSSQYNYD
                     ISDAFLVSIYNDSGTMDIEKEITLSSGTWDGLEGGETIMANIEPTTSALTEDSGAWIN
                     VTLRSTICPLAATTYYGCHR"
     gene            434..892
                     /locus_tag="PLK55_00010"
     CDS             434..892
                     /locus_tag="PLK55_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35355.1"
                     /translation="MNFKKKAIEPIVATILLVVVAVILVTIVLAWGKNFTTGGLNEAG
                     AITSDACKGATLSLESCDWNSDTNKVVFTLKNTSDTYTFDVNGFYINVADSSTVTESD
                     LDNVSSTTALGPGASLVEDITVSDIVATKVKLNVRSITCPTTAVSELECS"
     gene            907..1377
                     /locus_tag="PLK55_00015"
     CDS             907..1377
                     /locus_tag="PLK55_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35356.1"
                     /translation="MNFKKKAIEPIVATILLVVVAVILVTIVLAWGKNFTTGGLDEAN
                     NLTNDSCAGATISLQSCDWNSTSEVATLLIKNTSSTYTIAADDLSCNVTDDSNALNNH
                     QTIPTATTAAILPGASISTTCTASSLAAAATKVTVNVRSETCPTTAVSEIKGCS"
     gene            1400..1849
                     /locus_tag="PLK55_00020"
     CDS             1400..1849
                     /locus_tag="PLK55_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35357.1"
                     /translation="MISKKGVSPVIATILLVVIALILVGILLSWGQNFVNKNTSDADS
                     AIDRKCIGAGITINNCDYNSLSETLTFIIVNSGKVNFSADQNLSLILIDADTNLVNEH
                     QDIIDGNAFNRGDSFRIEIDEYVATTPIDVEVRSTQCVNYFARVSCN"
     gene            complement(1850..2353)
                     /gene="endA"
                     /locus_tag="PLK55_00025"
     CDS             complement(1850..2353)
                     /gene="endA"
                     /locus_tag="PLK55_00025"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HOZ35358.1"
                     /translation="MKVMLEKNSGLIKDNNLKSQITQKGFGFRKGLEMYLDLFECFYF
                     LEKKKIIIVDKNNKTISTKELEKTCNKEISDFSEKYLVFKELTNKGCVVKDGNIFGFD
                     FRVYQKSKAGEHTHTEMVVDVFKSTKIATREIIKSERLATTIRAKFVMAIVDKEERIQ
                     FIKIEKI"
     gene            complement(2369..2656)
                     /locus_tag="PLK55_00030"
     CDS             complement(2369..2656)
                     /locus_tag="PLK55_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HOZ35359.1"
                     /translation="MNTDVKKYCIKYRGLLEKALTTIKINIENDSFLKDMADNYLEMA
                     NNYLLDGKVLEEKGDFTRALASYSYAYGWIDAGVRIGLFYGVDRNLFTLYK"
     gene            complement(2640..3377)
                     /gene="dph5"
                     /locus_tag="PLK55_00035"
     CDS             complement(2640..3377)
                     /gene="dph5"
                     /locus_tag="PLK55_00035"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HOZ35360.1"
                     /translation="MFYIIGVGLNPKQLTLEAIECIKKCTEVYIDNYTNIFSQGDLTE
                     LEKILEKKIIPLNRTELEQEKKYLKDESCLLVIGNPFSATTHFTLIEEANKRKIKTQI
                     IAGISIFNYRGASGLFEYKFGKTTSIVYPEGNYKPTSFYDCIIENLKNNTHTLCLLDI
                     KTDKQKFMTLFEGCQILEEIDKEKGEKLKNAVCVGLCGISGKDQEIITFDFLDYKKVE
                     SKSLPQSLIICGALNDIERDGINEYRR"
     gene            complement(3379..3774)
                     /locus_tag="PLK55_00040"
     CDS             complement(3379..3774)
                     /locus_tag="PLK55_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35361.1"
                     /translation="MALKFWNLQNANNGQIFVIDGIFALIFASLLIVMAQTLNSSSLT
                     ENNIELLKMQKINDLLITSQYLKIDNLEALENNYLLLFPITSGYIIINNNRKEINSFK
                     TEKTKILSNSIKYINNSNNKIYIEVGVYS"
     gene            complement(3737..4147)
                     /locus_tag="PLK55_00045"
     CDS             complement(3737..4147)
                     /locus_tag="PLK55_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35362.1"
                     /translation="MNHKGQAIVLDVLFFSLCLIFVLYFEILFFENYAKEQINNQKEI
                     DDLKSILFIDKLVSECNYLAFTEINHESLCYQNILDLKFLMFEEELKKEKICRIRAGQ
                     ITIYQNEITEIEMTIQRGVIFNNGFKVLEFTKCK"
     gene            complement(4116..4331)
                     /locus_tag="PLK55_00050"
     CDS             complement(4116..4331)
                     /locus_tag="PLK55_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35363.1"
                     /translation="MNRKGIITLELILCVLIIIIVLGFFININLEFKERLEKNRNLKE
                     IIKIIKTNYYNEKNWEKYESQGTSNST"
     gene            complement(4328..4837)
                     /locus_tag="PLK55_00055"
     CDS             complement(4328..4837)
                     /locus_tag="PLK55_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35364.1"
                     /translation="MNTKNKGFLSLGVLLFLLVFLSIAGNGRIENNLEFVVVKSQSTK
                     KHSATINFEEMVKKELSMPNNDQLTLNAQINAKVINYLSNQDAQTEEWFILNINTNKK
                     QKINLLELQEITKTIVLRPMPNVYIKRYTITNGIAKNKYLGFEQKTSYSKTTFTFPKN
                     YTIEVIVYT"
     gene            5001..6089
                     /locus_tag="PLK55_00060"
     CDS             5001..6089
                     /locus_tag="PLK55_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ35365.1"
                     /translation="MKRNIFVKDLSDTQVSIFKDESVFYSSYLPEDIKAREKEIQELS
                     YNLRPISENRKARNVLIFGPPGTGKTLISKFVIRQLSEFTQRAKSIYINAIEDNTRFA
                     VYSKLLTLYNSPLPRRGLGIDELISRVKEEMSKTDFIPVIVIDEVDKLEKSEISTILY
                     DLSRIEVSMKYFALILITNHKEFVITLDPRVKSSLFLSEIEFKKYSPQELKEILKERI
                     SYGLIPNAISEDMVGYITGYAAKNGGDARIAIDILYKSAKTAEKKGNLKIDKETIQES
                     EKLIDAVKLCDKLNYIEPIEKDILNAIPKEGIESGNLYAAFPKDSDRTVRRHLDTLEK
                     IGLIKTKDNISQKGKTRKIELTFSKELL"
     gene            6124..6609
                     /locus_tag="PLK55_00065"
     CDS             6124..6609
                     /locus_tag="PLK55_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35366.1"
                     /translation="MPLKPIKPTGIHLVRAREKALSTHSSKRSLELKKKISNKDLKEM
                     QARIKELKQELRSQEYRVSKFKTVRELNSVKQKLLKEKIRIQGKLLAAFDDYYSLNGE
                     LKRIYVDSLEVYGKDLLERQKMNSEILKMLNKITKPEEKDMILRIIRKNIKLYYHMDI
                     K"
     gene            complement(6618..7367)
                     /locus_tag="PLK55_00070"
     CDS             complement(6618..7367)
                     /locus_tag="PLK55_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35367.1"
                     /translation="MIKLIIKDAKEFYKCIDAIGVLINEGLFTIDKDKFYLRATDPSQ
                     ISLVDFVMPKTAFEEFIADIGPESANKVSFGININNLLQILAHSNADDKLSIEIKEGK
                     PSIQLEFIGKSKRSFTIPLIDVSDQQHPLPQIQFDVTVQVGSDQLLSGIKDAALFSSH
                     ISLKVGDEKFILETQSSKGKFKQEVEAGDIVISGNKARAMFPLDYLQNMIKNAKTTIT
                     LKLKDDMPIELNYTIGSAAIRYYLAPRVDNE"
     gene            complement(7384..8130)
                     /locus_tag="PLK55_00075"
     CDS             complement(7384..8130)
                     /locus_tag="PLK55_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35368.1"
                     /translation="MNKKNKASKKQPKKNFWYYLDPFNYLDMALEKWIGKPEDFWKST
                     IYWLCYICLAFFLAIAIYDILGWILGVSMPMAIVYSSSMEPNLHRGDIVILTKANNLK
                     LEEITINENITNKDLKDFAELNFFKNKFGIEEIEYIKIGDKNISLLESIENNNSIIVY
                     PSNVTGKDIIHRAIVKINAKDGTFLLTKGDNHKTNVYIDQDCELEIINNEITIQKPCL
                     NIYPIKEKDLKGKKLGKIPYIGYIKLVLFQ"
     gene            complement(8127..8408)
                     /locus_tag="PLK55_00080"
     CDS             complement(8127..8408)
                     /locus_tag="PLK55_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35369.1"
                     /translation="MDIELINKGKNEIEFMIADEDSSFFDMIVDIASGKKDVEFVSKK
                     EEDNLTGRFTIYLRTRDKAAKDVLLDCIAEAEEKFNGIIKNLEKAVAKK"
     gene            complement(8410..8835)
                     /locus_tag="PLK55_00085"
     CDS             complement(8410..8835)
                     /locus_tag="PLK55_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35370.1"
                     /translation="MSNLELSTILANIRKKEKLNVGDVVIGEIVVVKDNMVVVEIVNA
                     GNKVLSPADMGIIFVKNIDTKFVESTSACFSKGDIIKAKVMEVGPYEYKLVTNNQELG
                     VIRTICKKCKAAIGASPNGEVICDCGNTIIKKFAKNGDF"
     gene            9244..9819
                     /locus_tag="PLK55_00090"
     CDS             9244..9819
                     /locus_tag="PLK55_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOZ35371.1"
                     /translation="MAFTFIFFVVNTETREEIFATQLSEIEKITDSRSSLTGQISGTE
                     LKNNNAFSNAFNLIFSNNLGVVLRAGLLSLFYGAGAIYLIAWNASILATAIANDILVN
                     LGGVVGVSGIFEGIGNALVTLIGYIPHGLPEVLAYILISLAGAIFARDLMKGLFFTEF
                     RWVIIKDIILLVIASIVLLLIGALIEASYFI"
     gene            9863..10867
                     /locus_tag="PLK55_00095"
     CDS             9863..10867
                     /locus_tag="PLK55_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35372.1"
                     /translation="MQDYNYLLRFPFSKQAKEYISSKRIDLLSVDYKVLEKVKKFLID
                     EINQDYKNLEKYWFNISKIDDEIIATSYVMIYPLSKIIISIIDNTPLTQSFANYYQKR
                     FMYFSKREDLENLKEIITDVCPQLFYDSYKEQYFISLIDLLTLDLGEDYKLQYTNLKE
                     GNIFFPSKDALLEFLSVVLKKRILRTTEINKKEIPKSFLEISEIVKKHFLSTQKARED
                     LDYRFSGKLSNGDFPPCFDKLYNDLLSGKKLSHIGNYHLAVFLAGVGYTYDDIINIYK
                     NAPNFDERIAGYQIKKIIEKKYSIGNCETLKSNDLCVADCKVKHPLQLLRKQNLTEKT
                     "
     gene            10864..11565
                     /locus_tag="PLK55_00100"
     CDS             10864..11565
                     /locus_tag="PLK55_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35373.1"
                     /translation="MKPSKIKILRAKSPIHERLIDERGRRRIDGDMDVQGIIEGDLTD
                     TWDKPGRDISKYKLQGILTRRGLNTDIRALKKRETPINIMVLGPGEGAEVLLLNEKLK
                     GTKIGIDTLGLTNNLSPEAKEIIRKDYSPEKLTAANIFENMNHLKMVRKYDYVYSRFG
                     PLSHTHQPEIVILKVASLLKPGGFARFYPIVFSTPMEIKEIIINCQKYLAKRGYKKAI
                     KFKVFADNLIIIREK"
     gene            11572..13113
                     /gene="gpmI"
                     /locus_tag="PLK55_00105"
     CDS             11572..13113
                     /gene="gpmI"
                     /locus_tag="PLK55_00105"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOZ35374.1"
                     /translation="MFGFKKKKTNNNKLLLIILDGVGINKPYPGNAVTLANMPIFNSL
                     RTNYPNMLLGASEEYVGLPKKQMGSSEVGHFTIGAGRIIDSEIVRIDKAIRTGEFYKN
                     IVLNAELQKIKGFQALHVVGLLSDGGVHSSINHLFALLEVINRHNNLSQVYLHLFTDG
                     RDVDPKFSKLFVSELLKRIAGNNRIHIASICGRYYGMDRDNRWDRQKKLYNLIVNGIG
                     ERSSDPLSYIEYCHSKEITDEFLPPVLFNENGLIKNNDTVIFFNFRSDRAREFTKMLM
                     DKSFDKFQTKKMFLNFLSFTEYDSKFKNVKVLFPPEKQKAGLGKIISELGYKQLRIAE
                     TEKYAHVTYFFNLGEEHPYKKEDRILVPSPSVATYDLKPEMSAKIITQRLIDKLNEDY
                     KLIVVNFANGDMVGHTGDIQATVKGLEIVDQCLEQIMKRVNLDNTTVIITADHGNCDE
                     MITSDHKVTCHSINKVPFILISNKEHKLRADNHESSIANVAPTILKLLGEEVPKYMSE
                     DLLDY"
     gene            13126..13602
                     /locus_tag="PLK55_00110"
     CDS             13126..13602
                     /locus_tag="PLK55_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35375.1"
                     /translation="MKILRKLFSKKPKSEKIVLDKIKTLKKEKPNNGNYHYLGGNWIS
                     KEAGSYHITYRQKGKIISLFDLRKTEKRKEINPKEYRDLLEKSIKNSRNLGCEMIVIK
                     TWIFAKYPELKEFGFLEIKPGSEAKFSEFISKRKITEEKLLSNPENSRYPLYYLKI"
     gene            13612..14646
                     /locus_tag="PLK55_00115"
     CDS             13612..14646
                     /locus_tag="PLK55_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HOZ35376.1"
                     /translation="MNILSLQKDGKVIFIPQTLEDLWVIKSITDLGDVVSGSSYRRIR
                     GNEADNESTRKPVFVSIAIEKFDFSKELNSLRFTGKIIDSKPEELAPLGEYHTLEISL
                     SKSYTLKKPHFFSHQLDLLENNSSVFSSSILLIALDDEKSNIFELTNIGIKDIAVIDS
                     GKPGKRYSSDFDFSSFFSEIFSIVSRYDCQIIIAGPGATRSKLSEFIKQKKSSLKILE
                     LSIQNTNKSSINELFSKEEVSRFFENSIIYKEQKVLDSFLENLGKNNNKVAYGLQEVS
                     KALDVGACESIMVSEKLWKTELDKVQELIRKADIVKTKVHIVDEEHEVSKSLRSFGGI
                     ISTLRYPLSF"
     gene            complement(14673..14942)
                     /locus_tag="PLK55_00120"
     CDS             complement(14673..14942)
                     /locus_tag="PLK55_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35377.1"
                     /translation="MGKAMSNKIKAKAKIIYNEFKEDFGTEFKANKEFIKTMNLPLSK
                     LTINLMSAYITRTVKKEKKEQEALKIKVDPNAIPTRKPINRNITH"
     gene            complement(14956..15162)
                     /locus_tag="PLK55_00125"
     CDS             complement(14956..15162)
                     /locus_tag="PLK55_00125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOZ35378.1"
                     /translation="MVSKLTKYEITRLISARAQQISCGAPALVKVHKDSTAYTIAKDE
                     FDTKTIPLAVIRHINNSKVVITVN"
     gene            complement(15254..15769)
                     /locus_tag="PLK55_00130"
     CDS             complement(15254..15769)
                     /locus_tag="PLK55_00130"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease family protein"
                     /protein_id="HOZ35379.1"
                     /translation="MELNEIMHLLFFPGIVLHECAHALACLMFGVKIKKIKFIDKSGG
                     YVVHEDSKDYKIIIISLFPFFFNIFISLICASIIKVTSEPFYIFLLIWIGIGALFFCV
                     PSDQDTDNSFDAIKRTYTKKQSIWWLLLKIVFSPFFLLLIIILALFKIFDKVIAIRLI
                     LVGFWLYLLVI"
     gene            complement(15784..16164)
                     /locus_tag="PLK55_00135"
     CDS             complement(15784..16164)
                     /locus_tag="PLK55_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35380.1"
                     /translation="MKGFVTLEYVLLIAGIISVLGASIIGIINLYNRNITANDNYRLK
                     GFCKDLKAEIELFEIMPEGKIEIEPGALSAWQIEKSNNKITLKNNQKTCEIRTTLSIN
                     CTITEITETQKITLTKQNNILNID"
     gene            complement(16167..16346)
                     /locus_tag="PLK55_00140"
     CDS             complement(16167..16346)
                     /locus_tag="PLK55_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HOZ35381.1"
                     /translation="MNLIKDCKGQISAEMIILLAAIVAIVLVIITQLQKTTTKANTTV
                     EKTSDKIFDKISDIE"
     gene            16450..18672
                     /locus_tag="PLK55_00145"
     CDS             16450..18672
                     /locus_tag="PLK55_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOZ35382.1"
                     /translation="MIKSIVLENWKTHQNTKVSFEKGTNIIVGKIGAGKSSIVDAICY
                     SLYGVFPSLQNRKITLAETIMFKPVKRKEAKLRLEFEHDSKNYRIEREVFAEKVNIAK
                     LFLEDKLIAGPKQTDVNERVSEILGIDYDLFVKIVYSEQNEIDYFLKIAPGKRKEQFD
                     TLFGINNLEKIKVNSRALKSYLDTELDAIQRLIAQLKSQISSFNISDTEKSILENQEK
                     IKQLLDSTSNLKSKEEGLKSKVEILKVQKAEYDSLNQEIKILDYREQELSKELSFLET
                     QKTNFFLLSKDVLLEKKQILQKQLKEIQEQETKLKSIASQLFIFANQLEQIDKSLDVS
                     LDLSKINLEKGKLSEEINSTIQKQAILNSQKSENLKAIFELEKGFSKCPVCDSQLSDF
                     QISEKLKLKKDLNAKLINDVLVLEKAQLSLKQEFSLLQIKERKASEQHLLMDRKQRIL
                     EEKKKLEEQEKALGKPQDISVLDKEIDSITQAIDYQTKKEQLSGIIEKQDFFSKKLSN
                     ISYDDNNYLSLLADLINQEEKLNNNAVQISLIQGIIKNLESTKAQYEKLSLQLQEKQA
                     IITKLETKQKDMTFFVAALENSQEHLRRVLVDNINQTLEIIWPKVYPYGDYLSARLRA
                     ENDYVLEVLTLQKEWIRVEGLLSGGERTCAALSIRVAIALSLTKKLGLLILDEPTHNL
                     DSKTITMLSSILDKELPELVEQIFIITHDQKLLETSNSSKYIIERDKENDGVSFVKEE
                     "
     gene            18674..19348
                     /locus_tag="PLK55_00150"
     CDS             18674..19348
                     /locus_tag="PLK55_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35383.1"
                     /translation="MPIKRTTFKSQIESVPGRTKHEIFGYKKPREKFIDKTIKIDKYK
                     VKYDEKHKYTTGEYTEIHTHLCNPKLATESTPSPHDFLGWIDDYLTTSGKKNRAVVSS
                     IHFKNGEELGRVHVLFKKEALNNQLRSHIYDSYLEFARGNGRQIIPFEKFNADLFFIP
                     ESGKVRKYLNTMIEKDFDFEKRLKVSKTKDLREIFVDIEKKWGIIIRPFSHPGHAFDY
                     KKGSFY"
     gene            19395..20645
                     /locus_tag="PLK55_00155"
     CDS             19395..20645
                     /locus_tag="PLK55_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35384.1"
                     /translation="MASTEISQFTVSYLRDLFRSYYAHNPVTSTIALEKREVGIGDFG
                     KKISARHLAFSNNTELNRFLVDKTPFYISYSTAYYKYPERRPMENKILEGSDIVYEFD
                     ADDFNLPCKEEHDVWFCPTCEANGRGHKDACPECQGKTEVIEWTCDKCLGHAKAETLR
                     LIDFLETEFKLDATSFIISFSGSKGYHLRITDKSIILLSKSSRIQMINYILGKDISLE
                     KLGFEYKGKQWYCPNPDTNNGWAGKLLVFLKDYISNMTEAQLRNDFGFSKIRASKILE
                     HKEVILNKMYNNILWHNFSSDSKQDAKFWEDLLALAISKLSFRIDPTSNDIFKIMRMP
                     DTIHGGTGLLSSKIKDREALEHFDPFADPVLFRPDKERNGEVTRPIPKFRLLEKKFGP
                     YSLGEKIELPEYASVYLLLKGVLK"
     gene            20642..21199
                     /locus_tag="PLK55_00160"
     CDS             20642..21199
                     /locus_tag="PLK55_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35385.1"
                     /translation="MMDYKVDYDELSRIYRLETKSPKLTKLDASFYKELKKYLTLEKT
                     KYLSALESLSPADLKKFEMLKQTVNKIREMRLKKCLNLCLTYSRTNDLSEDNLIDFEI
                     DFVKDIKKLLDKQAEYTESLFGTKTKKEEKRVPLVKVTALSSIPSFIGSDMQEYGPFD
                     KDAICEIPENIFLILSSKNIVQKIE"
     gene            21217..21990
                     /locus_tag="PLK55_00165"
     CDS             21217..21990
                     /locus_tag="PLK55_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOZ35386.1"
                     /translation="MTEYPKISDTVICKVTKVTDYGVFVELLEYDNIEGFIHVSQVSS
                     TWVKNIHNHAKTNQIRAAKVLHIDNYKNQIDLSFNRVTPAEEKRKISDYRLFKRAQSL
                     LSAIAKELKVSDEEVWSAVAEPILEKETSLYSGFIHILKNGIDSFPEIDKKYTIKLVD
                     ILSKSITIKDKKIIGTLEISNSTENGIEIIKKALKKITDEHKYVSITYVSAGRYELSV
                     TAKDYKTASKQFNEVTNKLEKSLKGSNIKIIPQEKSKAN"
     gene            22004..22798
                     /locus_tag="PLK55_00170"
     CDS             22004..22798
                     /locus_tag="PLK55_00170"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOZ35387.1"
                     /translation="MATKIDVLKSQKLRNPVMLVGLPGIGLVGKIAIDYLISELKPKA
                     TLYATIISDTFPPAVHAKASLLEMISDNIYLYKGKSRDFLFVAGPVQPNLTSISNSQE
                     HYEFSESLAEFAKKQGVKEIYTFAGLNIGEKRLNQKPRVLAVSSDKDTKERLKKKKTD
                     NIIFDDTQGDTLISGVAGLLVGVAYYNHKISGCCFMGETDQKLIFGDQGSARSVVGVI
                     CALFDFKVDMKRIDIQAKKIEESFTEIQNKIKELQKKKEEPVSYIR"
     gene            22808..23656
                     /locus_tag="PLK55_00175"
     CDS             22808..23656
                     /locus_tag="PLK55_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35388.1"
                     /translation="MHRLVKIRKPPVNAEIKIVRHRVTTEKLDSSNFNSSFISELKFS
                     KDAKGKPLKIGEGLHEQFLGTIIFKDGSKKRVAIKRFNELLKLDDFSAKKYQHVIDAL
                     RQIELEHDVVFPNRQIGKAKMIPKMAMVKIQEKGKDPEWVLVSQAFVKNGKSKLKPNN
                     DVIINKLNISEYTWGLLKLGESGFMKESSLGLVADLFAHHKSGSLIPIDMDFAQLIDF
                     KKRDRNTRANDILRALSQQAQSISKNKDNINSVYKTLVIELLKHKMDPKVKDLLISYR
                     KKYLIK"
     gene            23661..25202
                     /locus_tag="PLK55_00180"
     CDS             23661..25202
                     /locus_tag="PLK55_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ35389.1"
                     /translation="MTTKEEKGIVISTLDGPSTTKLSFVCTKNDVFKGEYIQIDYSQG
                     SLIGIVTDVIKRNRYFENSDSVKDFENNDIDIKRLFPSDEWEFLEAIVKPLGVYSVAN
                     GFTRTNLPVSPGQKVFLADPIVLSKFLHFDKSGVFLGAIEHPHLDVTLNVDRLLQKHL
                     AILAQSGAGKSYLTSVIIEELLDRTKEQGRIAVVLFDTHGEYSSFAQKPTDKEKIDYS
                     SKVTLIDSKDVKIGCTGLSAGFYSTLIPELTAPQKIRLSKVITKLNEDKKHGAGPYSL
                     STIVKEIENSGEDDKTLKNLRDRLLDLERFRLFDKIDYPSINDIVSPGKLTIIDLSRE
                     IDKKKKQIILYYFANKLFRARMENNSVPPFTLIVEEAHQYAPEMASRENALAKNILET
                     IAREGRKFGASVWLISQRPVKLSTTILSQANTHIILRITNPYDLKHIGESSEGLDQNS
                     INMITSLRVGEALIVGEASSYPVFFKVRKRKSQESHLDVSLSGLAKSFEDKKEKNNKE
                     LDDLI"
     gene            25251..25697
                     /locus_tag="PLK55_00185"
     CDS             25251..25697
                     /locus_tag="PLK55_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOZ35390.1"
                     /translation="MSWNELEGLSFELIKKIKEKNKDKQIDIVICVSRGGLFLGKILS
                     EMLNKPLAVISAKYLGDKYTVGTISSIYPVKGRVLLVDDVMEPVVQDIIKVIKQNKAV
                     TSVELACLFYRKKNKNFVPDFSINEIHNEIWITFPYQTECLKEHSY"
     gene            25699..26871
                     /locus_tag="PLK55_00190"
     CDS             25699..26871
                     /locus_tag="PLK55_00190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ35391.1"
                     /translation="MNIAIFTDNFYPQINGVVTAVLNLAKGLADKGHKIYFVAPKYKG
                     EKEFTYPNITVRYVASKPAGFIYKGLKIVSPISRRTKKFIKDNNIDVVYFVTPWTIAA
                     KGIIIAKQLKLPIVGTYHTFICEKECLKHLKLNYNFVIKFAWKYTNLYYNTCQIVTCP
                     SALTKELLLKNKCKRPVKVISNGLDTSSSPKSEIQKIKKKINSKGKTLLFVGRLAYEK
                     NLLYLLDCFKEIHNKRPKTKLVLIGDGPQKKEILKKIKELKLKDSVKLLGAVKYQDLM
                     TKGYYGACDIFVTASEIETFGLSVLEAQANGLVCVGVNKSGIKNLIKSNYNGFLVNSN
                     DKNAFSQAAIKLIDNKKLYSKFKKNNKKLLEKHKMENVISEYEKTFKQAIEMYKSS"
     gene            26915..28141
                     /gene="prf1"
                     /locus_tag="PLK55_00195"
     CDS             26915..28141
                     /gene="prf1"
                     /locus_tag="PLK55_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HOZ35392.1"
                     /translation="MDESQVLLLKKKLKEIKTYKGDGTQLISLYIPPNADRSSVTKQL
                     TDEISQSSNIKSQQTRKNVQAALRRLTNYLRQIDFKLPETGLVLFSGNVSENPAKNDV
                     VLLTVIPPKPLTTKLYWCDSEFHIAPLEDMSLTDEVYGLVTIDKREATVAVLTGKSYH
                     IVVHETSGVPGKTRAGGQSSVRFERLREKAAQDFYRRVGERMSISMVDIPKLKGIIVG
                     GPGMTKNEFLEESELDYRLKNMIMGTVDTSYTDESGIKEMLDKAGELLKDASIIKERQ
                     LINKFIEKVATNGLATYGYNEVIESIMQGKAETVIVSEGLEWSVHKYQCTSCGQFTPK
                     VIRDKNKQVTVPTTCQYCESSTNEEEVEIADLYEYLVDLSYNFGTKVVLISIETPEGN
                     QFLNTFGGLGSFLRYK"
     gene            28150..>29413
                     /gene="truD"
                     /locus_tag="PLK55_00200"
     CDS             28150..>29413
                     /gene="truD"
                     /locus_tag="PLK55_00200"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOZ35393.1"
                     /translation="MDFKYITTTKGVGGRIKQFCEDFVVEEIGYTYSTNLTYLPDKKV
                     PEQDWQKIFEEKKDNDQLLVDMEKFNLSTTTAINEMSRFLRTSKSRFGYAGLKDKRAL
                     TVQRISIFEPNMERLSKFYFKTIKIYNPVWSKNRVDIGDLKQNRFTITFRKINGYSKE
                     ELERLFLDFKKQIEKTGLINYFGEQRFGGVRDITHRVGKLILQNNYKDAVMLYLTEST
                     DLEIPELQKARRELKEDNNFKKHAYDFPSGTGYERVMLNYLANNPDNYLGAFKALPKA
                     IQYLFIHAYQSYLFNEIINERIKRGYGIEKIEGDTIINDEVAIPLFGFSSKFSEGLAG
                     VIEHEILDREGVTIDAFFNKDHSVFSSKGDYRPIRIPVYDLQLLEIKEDEKNQEELPG
                     SLSLTLSFTLDKGNYATNLARELIKPAPE"
     assembly_gap    29414..29513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29589..31736)
                     /locus_tag="PLK55_00205"
     CDS             complement(29589..31736)
                     /locus_tag="PLK55_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ35394.1"
                     /translation="MTKELISEILETNGYLSLNPVQEKAAKHIGENLLVCAPTASGKT
                     TIFEMFLLDFAVRRKKKVIYISPLKALTFEHYHDFKKKYAKKHDLRFGISTGDLDSSS
                     KNLSSYDVLFLTFEKFDSVLRHEPEWLKDIGFVCIDEIHELGSDRGTTLEILITQLKV
                     NFPKIIFLGLSATIGNSQALAKWLNAELVTSNYRPIPLDIGINYGNSVFFDERKIEIE
                     ATGKDDNLGGIIADTLKLNKQLIVFCNSRSNTMSFATKYRELVKRFLNENEQTELRKI
                     ARTAGDALETPTKQCLSLYSDLLCGTAFHNAGLVYKQRQIIEEEFRNGKIKIIFATPT
                     LAAGINLPAFRVVITTVYRFSNGSMVLIPINEFHQMTGRAGRPKYDSKGEAIVIVNKE
                     ADVQRVYNSYVIAPPTDIESQLSKINNLRTQLLSIILINNLKTTKEIHAYMEKTFYHF
                     VFGGDQEIKENVLEILEEFNDFGFIEKAKEEIKLTEIGKKVCLLYIDPLSANNILKDL
                     EIKEKLKDIEELERLFVISNTSECYPYLKVKKGKEDDVFEVLEYLKPKIYFDYEDLNL
                     LEKITLSQMLKDWINEISENDIIEKYGTTPGQVQEFINKAIWINHCTQELIKTTKKSL
                     KLYKEYSDLELRLKYGIKKELLPFVELKNIGRVRARKLFSSGITGIGDIKKNPEKFLQ
                     TIGRIGLEALKELKIEIPEAYQDKKEKTKISDF"
     assembly_gap    31852..31951
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32226..33485
                     /locus_tag="PLK55_00210"
     CDS             32226..33485
                     /locus_tag="PLK55_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ35395.1"
                     /translation="MDKQYIERIILDQSEDLKKMELGFTRNKLQDIDSHIKSKINIVI
                     TGVRRSGKSILLLQIMDKYYKDKFYYLRFADERLSDIQIEDYQKIYEVFLANFNKTNI
                     FFFDEIQGKPKWDQFVNRLYETGNKFYITGSNSELLSKEISTYLTGRHLDIELYPFSF
                     REFLDFKKIDLDYRYTKNLIKIEKEIVNYLKIGGFPQVVVENNTSLLEEIYEDIINKD
                     VLLRYNINDVSVLKKTAIFLMSNIAKEFSYNSLKKMFNLGNSNTIRNYVYYLSNAYLL
                     FELNKYDYSFKKQENYSKKIYCIDTGLINKMAFSFSENLGRLYENCVFIELKRRGKDI
                     YFWKDKSNSEIDFLMLEKNKVVSLIQVCYNLNDLKTKEREVNALLSGLDEFKLKEGII
                     ITKDLDKEEKIRNKTIKFIPLWKWLLE"
     gene            33620..34480
                     /locus_tag="PLK55_00215"
     CDS             33620..34480
                     /locus_tag="PLK55_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOZ35396.1"
                     /translation="MKNQKLKAITPIVTTILLVVVAVILVTIVLNWGRVFTTSSLSKT
                     QGIELTKSDAVSFVYPTQLKDGIIQFNYSPPSSESFGDIIITSYKVLSDSGETPDTNL
                     SSNYTLKQGSNLIDLTGFSSLGLDSSSKVTLVLQTTDNTYLDIKNITNYYAVPVLLPY
                     VLTNSGDKLYIHPSDSPTALTWADAGPYCENLDSNGYDDWYLPSMSQLADIWLACPDQ
                     TKSNICMNNNINKDNLPYTETWVDFIADYYWSSTECGADGAYDIAMNYGGIGINGKAK
                     GLYVRCVRDQ"
     gene            34625..34987
                     /locus_tag="PLK55_00220"
     CDS             34625..34987
                     /locus_tag="PLK55_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOZ35397.1"
                     /translation="MVSYENLGVYKSSFDLCVYFEKIVKNFNKGNKFTIGADLKNKSR
                     EIAMQVVRANISSTKIKEIQELIILIEEQKLIVRLCQETNGFPNKNSYGYISKLLTNL
                     TTQANNWLLCSQKREQTE"
     gene            35176..36414
                     /locus_tag="PLK55_00225"
     CDS             35176..36414
                     /locus_tag="PLK55_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HOZ35398.1"
                     /translation="MNNGNINNNNKTNNNYVRCVREHEFTFQELHKAYMTCRKNKRNT
                     INALKFEVDLESNLQELVFCLNNKTYKPTRSVCFSITDPKPREVFAADFKDRVVHHLL
                     YNRLNPIYEKIFIYDSYACRKNKGTLNSVKRAQTFTRKITNNSKEKAYYLQLDIHNFF
                     MSIDKDILFGIVSRKIKDTSWLWLTKQIIYYDPTEKYLQKGSDWKQSGVPKHKTLFNT
                     KKNKGLPIGNLTSQFFANVYLNELDQYIKHKMKVKYYIRYVDDFIIFSKSRKELLSIK
                     KEIETFLKEKLSLVLKPLTILKPISSGINFVGYIIKSNYLLPRNRVVNNIYKKINLFK
                     KDSIYLENKALVYDFKNSVYFIKSINSYFGYLKPSKHKNLLKDIKTKNRFLNILFIFE
                     NHKIKNKYQTNFINLKIKAV"
     gene            36603..37283
                     /locus_tag="PLK55_00230"
     CDS             36603..37283
                     /locus_tag="PLK55_00230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOZ35399.1"
                     /translation="MINNNDIIELDLIIKDKNTNSVLDTTHEDVAKEGNIYDSKTQYK
                     PLVVVFGKGELLKAVEENIKDLDIGKTKEFSVTKDKAFGDRDPKLIQLLPLSDFKKEN
                     IDPKPGMFINLGNRQAKVLNVSGGRVQIDLNHEFAGRDLDYKITLKKVCANDNDKSQA
                     LFEKYFFFMPKDKAKVQVKEKTLELIIPGAMPKEIEYLKYIFIQSVFDTCNFENIHFI
                     ENYEKKKQ"
     gene            37264..37905
                     /locus_tag="PLK55_00235"
     CDS             37264..37905
                     /locus_tag="PLK55_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HOZ35400.1"
                     /translation="MRRKNNKGGREILETYKTGTTTVAILAKDSIVLAADMQATMGNL
                     QSNTEMTKIYKINDQLAVTIAGSVGDALVLVRFLKVQASLFDLEHKTPMTPKACATLL
                     SNILNANKMIPYVTQFILAGSVGGLALYNIDIAGGIVEEKDFSYSGSGSELALSVLDS
                     HYKKGLTTEDAVKLAKDAITSAKKRDIYTGGQGIKVVIVKKDRIEELPITKYN"
     gene            37963..39849
                     /locus_tag="PLK55_00240"
     CDS             37963..39849
                     /locus_tag="PLK55_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HOZ35401.1"
                     /translation="MEDEFKTIKDYIEKFLAKKNINVSKIDAEGPEIGIYTDTPLIFF
                     EEKAIIGELAEQLKKRINIRSEKSILMDPAEALKIIKEIVPEDADVKSTFFDTSFSEV
                     VIEAIKPGVVIGKQGMISREIIKRTGWIPRIIRAPTEQSEILKRVRLTLLKNSSERKT
                     FLKSVANTIYSPPVSNNDWIRVTALGGFREVGRSCILLETPDTKILLDCGLNASDKNE
                     EAPYFDVLRFPLDELDAVIIGHAHVDHCGFLPYLFKAGYNGPVYCTEPTRDLMALLQF
                     DLIDVAVKSGREPPYLEEHVKEVIKHTITRDYREVTDIAPGIRLTFHDAAHILGSASV
                     HLHIGEGKQNLVYTGDFKFGFTRLFNNMDINYPRLESMIMESTYGGKEVTPSRMHSET
                     NLIDVINETTEKGGITLIPVFSVGRAQEAMIILEEYYRQGKLKASKILVDGMTRQASA
                     IHTVYPEYLKKNIKTRVLQNNSPFLSDIFVTVDDAKEGEELITPGTVILASSGMLTGG
                     PSLRYFQRFASDPNNTMVFVGYQARNTLGYKIQKGLKSMPVVEDGKNKNLDINMRIET
                     VDSFSGHSYRAELLNFLNMLKPMPKTILINHGDSCQEFANYVGRKYNVDTKSIQDLES
                     IRLR"
     gene            39856..40344
                     /locus_tag="PLK55_00245"
     CDS             39856..40344
                     /locus_tag="PLK55_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ35402.1"
                     /translation="MKILIAGIPGVGKSEISKVLGKQKKFKVINDKDYSIKNNLGYYE
                     VIDGYKEYFVGLNALNISVLKELKSKDNIIFEGHLWCELSKSNLKYFDKIIVLTTDKN
                     TIRERLEKRRYRLVKIEENIFCQEHKYIPEEFDKKNTKYIVIKLNNNLKENVKKISEK
                     IW"
     gene            40338..41129
                     /locus_tag="PLK55_00250"
     CDS             40338..41129
                     /locus_tag="PLK55_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35403.1"
                     /translation="MVRALSTAQTQSVREIKKNADIKKANKKLSVFVVTFFIMLIIGF
                     IAFKAYTYYQSTKFDFKETEKGINFYSKDMPIKDALQVSLDSNKVAISTNLLINYSGT
                     TNPVLEPSILATTIFVFKDKNTININNRLDENFSIIKCSTNYGDNLTSVDLNSTECLA
                     LFSQIESKLVINTPNPSLKESRVDIYPMEKTIIINSKSETDLLVSTYLLLKANYSDVE
                     SILFKVENIKKKLSNYLDSNSVKDTNSTTPPDYNNQDSNLEDLNN"
     gene            complement(41126..42106)
                     /gene="dph2"
                     /locus_tag="PLK55_00255"
     CDS             complement(41126..42106)
                     /gene="dph2"
                     /locus_tag="PLK55_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HOZ35404.1"
                     /translation="MSLKDIIITEIKKTKTEIVSPLGIQIPEGLKQFSSLILEELKDY
                     SPALFVDPCYGACDIKFREAKDLGCKTLLHFGHRQLPGLKTTKAVKIIYVPLNFEYTN
                     EQIDFIVSEIKKHGLKEISLVTTVQYLSNISVIKEELLKAKIEVIESNKTKRLQEHQI
                     LGCDCSNLCDNANPIVFVGDGWFHINNVSYNHRKQEVYLINPLIGQSEKIAYDEKFLR
                     QRYAAVARALECRSFGILVSSKPGQNRLKLAKKIKTKLEKNGKIANILVSDYINENYL
                     LGIDVDCYINTACPRIAYDDFNSFKKPIISATEIEQIFNIKKEIKIDQIS"
     gene            42217..43581
                     /gene="cca"
                     /locus_tag="PLK55_00260"
     CDS             42217..43581
                     /gene="cca"
                     /locus_tag="PLK55_00260"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR03671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOZ35405.1"
                     /translation="MYVGIDKSKLDTYKETKKKILGLIKPTDTQILKEKEFTDKLIKK
                     AKGLPGKHLDVVLAGSAARNTNLKETKDFDIFVLYPKDTSKEEFTKEALDIGQNFFKG
                     YFSEKVYSEHPYVRGVIDGYKIDLVPAYKIDSTDEMISSVDRTPFHQAYIFKNMKEAQ
                     KDEVRFLKYFLKYIGGYGADPQYQGFSGYLCELLILYYGDFIKTIEHVANWQTPVRLV
                     LASEDIEHLGPFDDAFVMIDPVDKKRNVASAVSMTTLSRFIAASRAFLTKPTTEFFSN
                     NNKPQTYHQLVTWVNNVPLTTIEFDVEGLVKDIVWSKLRKYLTKLSNHLENQKFTILK
                     SNVYYEEKSKYAYIILMTDMLVLPKFEKATGPFVSDMSNSEKFIENSKAIYGPFVKDD
                     RWYCIKSRRFVEIKDVIEDFSNINFDIKCEIFVEQDIIKLYTKNTDVMPYFTDFFYPR
                     ENFL"
     gene            complement(43594..44523)
                     /locus_tag="PLK55_00265"
     CDS             complement(43594..44523)
                     /locus_tag="PLK55_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35406.1"
                     /translation="MAIGKTKAFKTFGSKTKTGNFFERLEKRKRQQISELESQSDLKK
                     TDFKTENRKFIDKQKVYLKYRTYRQNAKEIINQTISMLKRFSKDPKLRNAEFVFIDRD
                     ALPYMFIARELCSQYGFKKEQFKKILSSKEAEEHTDTKLIADIHPKEYMLLESNSLRL
                     KKMAKKIPLTSAIIELKKWIKQNINLKRPVVVIDSGYNGTSVKRLQLVLADIDPKIKS
                     YSAMFFATRYSKPKLDYYFGDVDINKFSITDIEGVPKFQGKLLKIEKGKTKRERISAQ
                     ELTTGDLDPVMAEVSMIALRNTLFRYKKEKGIK"
     gene            complement(44549..44622)
                     /locus_tag="PLK55_00270"
     tRNA            complement(44549..44622)
                     /locus_tag="PLK55_00270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44585..44587),aa:Arg,seq:tcg)
     gene            44711..44866
                     /locus_tag="PLK55_00275"
     CDS             44711..44866
                     /locus_tag="PLK55_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOZ35407.1"
                     /translation="MDKCTSCNKEVTTDYTQFKCPSCGKNKIIRCTRCKTTSKTYRCK
                     ECGFIGP"
     gene            44880..45149
                     /locus_tag="PLK55_00280"
     CDS             44880..45149
                     /locus_tag="PLK55_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35408.1"
                     /translation="MGDILTVYKLFPEEIEDTEKIQEFIKNNSPGTEFRLNTVDLEPI
                     GFGLSVVKVSYVFSDKVDGLLDKLEGFLKGISGVNEIEVIATTLI"
     gene            45205..45657
                     /locus_tag="PLK55_00285"
     CDS             45205..45657
                     /locus_tag="PLK55_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35409.1"
                     /translation="MFTKKGQLSIEVLIIIIFFITFIYVYNNLAEQTVYSLEVNKIKE
                     QELTIALSVNEFLEAQKNILADSTIVDYNATYRLPTIAIASRKLTSCFVDINLDTQQL
                     IVQTDYSNIHTDIDINLSSTDFNISGIISCGQTIVCDKPSTKIECRQI"
     gene            45654..46139
                     /locus_tag="PLK55_00290"
     CDS             45654..46139
                     /locus_tag="PLK55_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35410.1"
                     /translation="MIKTKKGQVMLLDVLFAVVIVILMFFLLLKFVETDIYKTNVDSR
                     TEELTSIGSIAYQKFLNNPKINCKVTDSQNSFYLPGTIDTSETITKADLGIPADYNCH
                     TDPTVDGCQATAFSGPSYTIQFPVVTCSTDLNKNTYIDYITDIYNPSTQLITLQVWRA
                     S"
     gene            46139..46432
                     /locus_tag="PLK55_00295"
     CDS             46139..46432
                     /locus_tag="PLK55_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35411.1"
                     /translation="MKSKAFIFSVTALYFVMLFVLFMGLITYTYHNAVYKQTTSVVDK
                     TASDYIQGNDSTSIPTNGWCVRRIVYDANTQSSTQSPREIKTYCEDYGTQRFI"
     gene            46410..46883
                     /locus_tag="PLK55_00300"
     CDS             46410..46883
                     /locus_tag="PLK55_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35412.1"
                     /translation="MARKGLFELVFILVMLVLMLPATMHHSAIVTYSAQAQANDLLLL
                     TDQAIADALADGSHSGCLASSNQVASYVNNLQTAYQDIGNNCEILVNVSGTNPYTGDA
                     NVTCFKNNGDTTTYIKKRMTFNKTVTATSGGGTCRVIVIDNLSSGANQVDYNLST"
     gene            complement(46880..48658)
                     /locus_tag="PLK55_00305"
     CDS             complement(46880..48658)
                     /locus_tag="PLK55_00305"
                     /inference="COORDINATES: protein motif:HMM:NF009945.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HOZ35413.1"
                     /translation="MAKNIRIAIVDKDKCQPKNCNYLCKRLCPGVRMGEETIIVEQGN
                     IFPIIDETLCTGCGICQNRCPEKAISIINIGMELDNPLHQYGKNMFRLFSLPTIENGF
                     ITGVIGRNGAGKTTAINILSGNLVPNFGDYSLPGDKEKVIEYFRGKAIQQHFEDLRDN
                     KITIAYKIQKVEDIPKAYNKKIYELLDGISKDEKYKAQIIKELELEKLLEKTPKEISG
                     GELQRVAIASTLLKAANLYFFDEYSTFLDIKQRFRIAKILREKITTENSMLLIEHDLA
                     ILDYLSDFVHVFFGQKQAYGISSNKKTTKKGINEFLDGFLKEENIRIRTNAITFSSTQ
                     DKGITKRLLFFEYPSFTKKLGSFLLEANEAKVNRGEVIGILGANAIGKTTFIKVLAGL
                     EKSEENNIDLKLKISYKPQYIDLDQEMLVRDLFSGKEIDQEILSSELKRQFDLESLFN
                     QKIGELSGGELQKVAIANCLSKSADIYLIDEPSAFLDVELRLVVSTAIKNIITKTEKC
                     AFVVDHDLLLLDYISDRMLLFEGAPGEKGFAKGITGIQEAFNSFLKIENMTFRQDPET
                     KRPRANKPGSQKDQEQKKENKYYYTT"
     gene            complement(48663..49898)
                     /locus_tag="PLK55_00310"
     CDS             complement(48663..49898)
                     /locus_tag="PLK55_00310"
                     /inference="COORDINATES: protein motif:HMM:NF003229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HOZ35414.1"
                     /translation="MKSFFDKYTPKSLEEIVGNKPAIETIRRWSEKHDKPLFIYGAPG
                     IGKTLVANLLAKEKKWAILTTDASDLRDKENTKNLLNAACTSNTLFGTTRLVLIDEID
                     SVADKKGGEDFGFFTELEKTFDKSKQPIIIIANDPYENKKLRPIFEKCEKIKFDLPHK
                     PSILKFAKEICDKEEIDYDLVALNSIIENAANDVRATMIDIYTLSFNKKITLEDTESL
                     GDRKKDEDVFKVLQKIFYPKDFYETRNVIDSLNINWDLLMLWVEENIPRQYKNANNLN
                     RAFDNLSKADINYSRIRGSNWVLLKYVFDYLTVGVAYSKKEREISSGYTPFQFPRVMR
                     MYTTKDKILQKSVVEKMKQRIHCSKRIILRDYLPFMLIIAKTNKCTTDLITHFDFTLE
                     EMKYLGAKITEKQYEKIVS"
     gene            complement(49938..50888)
                     /gene="sdaAA"
                     /locus_tag="PLK55_00315"
     CDS             complement(49938..50888)
                     /gene="sdaAA"
                     /locus_tag="PLK55_00315"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOZ35415.1"
                     /translation="MTEYILRDIVKLAKERKITIAEIVKEDERVSFKRSDKEISNKLN
                     NSINTFNRGITSGLKGNYKFKNLMFSSDSIKMANKKTRILGNTLHKACVYALAISENN
                     LNMGTVIACPTAGSSGIVPGCIMAVSQDYNCSEKDIQNALLTAAAIELSIIKNASISG
                     AAHGCQAECGSATAMAAGAIIHLLYKDNTNPEMVENAAALALKNMLGLVCDPVAGVVS
                     VPCRKRNATSIGIAFVAAQMALSGIKSYIPFDEVASAMDEIGNRMDDELKETARGGLA
                     KTRTGKKWETKKKKLLEKASKKKQSFADVLKNTKNEDKKI"
     gene            complement(50885..51301)
                     /locus_tag="PLK55_00320"
     CDS             complement(50885..51301)
                     /locus_tag="PLK55_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HOZ35416.1"
                     /translation="MPYISLLDLVTSEIVGPSSSHTAGVMNIGLKVSGKVKDIKAVKI
                     VLYNSLADTGDAHMTKSALVAGLLGFKETDPKIPFSFEYAYSKGIKIEWHHHIDEKYH
                     PNTVFLAVKNKANITRFFRGTSIGGGRIKFNEVASL"
     gene            51415..52131
                     /locus_tag="PLK55_00325"
     CDS             51415..52131
                     /locus_tag="PLK55_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35417.1"
                     /translation="MISTKNTNLLTKSLETITPLIQETNVRFKDEGIYIKAIDKTQIL
                     LVDFFMSKKAFDTYAVEPSLVGLNILELKNMLSRSFGEDKLQLNLKDQGLDVYLLGKI
                     ERKFYLSFLDLSEQNINLPEIRYEVEFNIAAGLLKEILTDINLVASTIIFKINDGKLI
                     IEATGEKGNIKTTLPEIKVKSKKNFSAKFSLSFLRNIVKSMDNDKVLLIKLAEDVPIY
                     IEYTLSKDCIIKFYLSSMLI"
     gene            52141..52671
                     /locus_tag="PLK55_00330"
     CDS             52141..52671
                     /locus_tag="PLK55_00330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35418.1"
                     /translation="MNSIKKFNFKTKNEFHQFIIKHTRFLADLIDIQNYSGIDYNFNK
                     DGLYKFLKADFKKGGSVFLIYDGKDPIGAIYGYNNVYGDREFTLSFFAIKKEYQRKGF
                     GKKLLKFAYDELLNTQGYKNVCMFSSSGTRAINKQISGLKNNGKLKPGPKSYKLDSTH
                     GTDKVYLIPKPRHILK"
     gene            52679..53371
                     /locus_tag="PLK55_00335"
     CDS             52679..53371
                     /locus_tag="PLK55_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35419.1"
                     /translation="MSRPIRPMGPMLKGGYSERLTKRVSRKKTNTVKPIKFNKPYLSD
                     YDTRLIKFRFAFENKYGFNIFKTKGQELHVFLPMTSMSPQGYLLKSFFEQTLPQARVT
                     FLVTPKTDALAKTKDSEFRRKALDNLENNLRRSLNIHDKYFVCFDYIDTGKTQINIMQ
                     ALNKIYNREVNYMAVKSPEGGAVTTHLKETDGSSIRLYHSKAKTKNIIQEHYNAGMEL
                     SRKKQFMLKLLE"
     gene            complement(53376..55301)
                     /gene="lonB"
                     /locus_tag="PLK55_00340"
     CDS             complement(53376..55301)
                     /gene="lonB"
                     /locus_tag="PLK55_00340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOZ35420.1"
                     /translation="MPKKNQNKTKTKAEAKNDVKELIYNDTSEIEIPKKMIDQVIGQD
                     ASINLIKKVAGQKRHIMLIGAPGTGKSMIASALAELMPTNKLTDIMCYPNDEDSHTPK
                     IVEVPAGDGKKITDATKIEVARQMGAGRIIGFFLPTIWLIISIVFWRLGWVPDVVFAA
                     MLLLSGFLMVSTSISMQMMQKERALIPKIIIDNRNRKEVPFIDATGAKAGALLGDVLH
                     DPLQSGGLGTPAHMRVVPGMIHKANGGVLFIDEISALSPKSQQDLLTILQEKKFSITG
                     QSEMSSGAMVRTTPAPCDFILVAAGNYIDLEKVHPAIRSRIRGSGYECHMNIDMTDSA
                     ENRNKIVQFVAQEISKDKKIPHFSKEAVDEIIFEARKRATRKNSLTLKLRELGGLIRA
                     SGDLALEENKKLVTRDLVIKAKSTAKTLEEQMVNKMMIVKKDYDIFKTTGYSVGRVNG
                     LAVMNESDAGLLLPIECEITPAASKNEGRIIATGQLGNIAKESVENVSAIIKKLTGKD
                     ISNYDIHVQFLQTYNVEGDSASISIATAIISVLENIPIKQDIAMTGSLSIRGDVLPIG
                     GVTAKVNAAKNAKIKKVIIPESNKEDLVLSPEVAKDLEIIPVKTIWEVILHAFKDSIK
                     KEELIKRINKTYAIKLV"
     gene            55388..56326
                     /locus_tag="PLK55_00345"
     CDS             55388..56326
                     /locus_tag="PLK55_00345"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOZ35421.1"
                     /translation="MSNKIKVFFLGNSASLPTAERNLSSMLINYNGQNYLFDCPENCQ
                     QQIMRSKQSIMKINNIFLSHMHGDHFFGILGLLSSFKLNNRIAQLNIFVPKNEKKNLE
                     QLIKLTIYDKYPPEYKINIVEVEKNIAYQDKEIKVEAISLDHTTRTNGYIFKVKDKVG
                     KFNKEKALKLKIPEGPLFSQLQKGKSIKLKGKTIKPEMVMDYKFKKIGKKIAYLCDTQ
                     VLKNIPKELIDIDIMVHECTFFVEDKDKARLVKHAVAEEVPIFSKKAKVKHLYLTHIS
                     SKYNDLEKREKQLQKKNKGQKINIVHGLKEIELNDY"
     gene            56390..57349
                     /locus_tag="PLK55_00350"
     CDS             56390..57349
                     /locus_tag="PLK55_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35422.1"
                     /translation="MAIAKRAAKRIAPKTLEKMRNRTAEKERMNAEKEKNKILKLKEY
                     REKTQEDLQYEARYGKLLSNLRKQKRSKIADKERFTPKIGLSEAERSKNMRNITQEKI
                     DALKNRINSIENAWKHLESQKYVPDNNNPQKGMIIRFLTEAKTKKQWKERGFQEGHIV
                     SEKQYKDALNRNRLAYNTAVNELNRAEFLYSLKRQERWLSGKNVGEFKEAHAKAIDVL
                     AQRYERGQINGETLKKFMSVLDSSVNRTLEQHRVLEKTKGSEYFDAHSRVAGKVLLSF
                     MKEANLKNFNESAKKFELKMSAIAGTIDKPNRSVELEWLYKRL"
     gene            complement(57350..58039)
                     /locus_tag="PLK55_00355"
     CDS             complement(57350..58039)
                     /locus_tag="PLK55_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ35423.1"
                     /translation="MGIKIRYLGHSSFIINMNGNNILTDPYFDNNDKCSYKRLIDCAM
                     PVTNIPSIDSILISHEHFDSFDIANTNLIVKKNKPKVIAHRSVLNKIDCGENYKVSMD
                     EYKTKTINDIYYTAYPAHHPTSFYPLSYLIADKKNSIYFSGDTFMTKEHDKIKADIAI
                     LPIGGKTTLDLGSAVSVAKKIRPKYLIPMSYNTFAHIKQDPYILKNKFENTRYTIKPI
                     VIEPGKEWKYK"
     gene            58165..58974
                     /locus_tag="PLK55_00360"
     CDS             58165..58974
                     /locus_tag="PLK55_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35424.1"
                     /translation="MAKKHILIGILFLVLLMPVYAVSLKDFDISINVSSPDKATIIET
                     WNVDAGSEEELSDFKSAILRNSGNLAELREINPDLNPHIFVDEINIRNFAISFDDFTK
                     KIRLEYTIADKCLINFLDYQDQIIWELNNHLFNQFIITGVFNIPTNSQISITLYDPLL
                     IGDVVPTAKITGRTILWGGSSSNEIRVLAIEKKPPKPSFVFSNLFSKDYLNKSYFTVL
                     FILLIIVLILLIFKNKVNSGIKKFITKHSVIKPRKQINDIVDFDFVSKKNK"
     gene            58981..60474
                     /locus_tag="PLK55_00365"
     CDS             58981..60474
                     /locus_tag="PLK55_00365"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOZ35425.1"
                     /translation="MNLKFQESDAIVYEYLKENKSAMLEELLKNTNYNQDSIRRSIET
                     LKLNGVIEENTTISYIYELDTNGKLALENGFIEELFCNYIKDKKIKVTEINNLEIPNL
                     NKEDIILAFGIAKRKELIEIIDGTLKTKKESKTQISKQKEMLSDISLKKPIIENLDFQ
                     ELLKRKNFILKKEKQNKEYKLLQIISYNIISDQQLYLTPDILKSGEYRNINFKEFEVT
                     ALPLPKEQGRINPLRQVIYQIRELFLQMGFKEMQGPYIESCFWNMDSMFISQNHPARD
                     IQDTFYLDLFGELPAERNLTAQIAEIHNNGWKTGSKGYQYEWNCEESKKLILRTHTTA
                     TTYRTFFNLSSEEKDNSKYFCIDKVFRNETIDFTHLPEFHQAEGFIIGDNLSLADLLA
                     FIKEFFSKLGIEKVKFKPTYNPYTEPSVEASGYQESTGKWIELINAGVFRPESLAPYG
                     IEKTVIAWGLGVERLAMLLYQKPIKEIHGDEENIDWLKEYIIPKREL"
     gene            60477..62150
                     /gene="pheT"
                     /locus_tag="PLK55_00370"
     CDS             60477..62150
                     /gene="pheT"
                     /locus_tag="PLK55_00370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOZ35426.1"
                     /translation="MATIELNFSDVKNEFNKDISIDEFEEVLFNYGFEIDSYNKVTDE
                     LRIEVTAERADVLSKEGLLRVLKVFGGHGKPLNYSAKKPTHILNIDNSVKLVRPYSVC
                     AIIKNLKLTDDRLKQIIAVQEKLHFTVFRKRTLGAIGVYPLDKIKFPITFCAKTPKDI
                     RFTPLGENKEMSGEEILKDTETGKEYAHLLLEKDKYPIFIDNSGQILSMPPIINSDVT
                     GKVTENTKEIFIECSGFDLVRLNQVLNIMCCMFSDFGGELHAMEINLGNSYAEKQIIT
                     PNLNEEKRLVTLANVNSLIGLNLDIDKASVLLEKMCYKTKKYGKDKIEVSIPPYRTDI
                     LHEVDIIDDIARAYGFNNIPLKMPKVFTVGERLKDSIKQDNIIQILVQCGLIEVSPLS
                     LSSKKESFENFNISFTKDKAIELGYSKDKSLDIVSPWLLPKLLKLLTNNQHMSYPQKI
                     FSCEKVVIPDKQKDVKSKTILHSAIAIANSKVSFTESASILVTLCNTLGYSLELQKKD
                     YPFYISGRSATIIINNKEVGHIGEINPEVLKRFDYNIPVVAFEIDVNDL"
     gene            complement(62137..62859)
                     /locus_tag="PLK55_00375"
     CDS             complement(62137..62859)
                     /locus_tag="PLK55_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOZ35427.1"
                     /translation="MPTVLTHLGFVEILEKKRPAFFKKLDLRYLVSGSLFPDYYGFYK
                     IQTKLNPEFFYEIRNKKGTTFGKRMYAIARTKAEKSFAIGFISHNVLDKHFHNYFNKN
                     GITADNPEDHLMLEFYYDCKFKNIRVSPVLYPDGIIEETLNRYYKNIKSKKAYVTKIK
                     LMGYYLFLKEIQKRIIHKKYMKKEKSYLDLLAVLFYKKPINLKKLLNPNKDLKNKHIK
                     NLEKVFAAAEKEMLKIISSINH"
     gene            complement(62888..63283)
                     /gene="eif5A"
                     /locus_tag="PLK55_00380"
     CDS             complement(62888..63283)
                     /gene="eif5A"
                     /locus_tag="PLK55_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HOZ35428.1"
                     /translation="MARDYEIKFMELGQVKEKQFILIDDVICKVDTITKSKPGKHGAA
                     KIRMTATAVFTGQKMNLLKPSSEDVQIPILDKGNAQVIADLGNKYQVMDMASYQTYEV
                     DKDEFTDLVAGNEIEYLKAGSMIKISRKK"
     gene            63421..63879
                     /locus_tag="PLK55_00385"
     CDS             63421..63879
                     /locus_tag="PLK55_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOZ35429.1"
                     /translation="MLKIFKKIFKKVPKENTRNNIAVFVDGPNMLRKEFNIDLSKIKS
                     QLQKDGSLKIARVYLDQYATDKLIEAVVNLGFESVITVGDVDVAMSVDATEAAFSQEI
                     DSIVFITRDSDYLPAILKAKRQGKKTIVYLVEEGSAVALKNTVDRVVFIK"
     gene            63888..64388
                     /locus_tag="PLK55_00390"
     CDS             63888..64388
                     /locus_tag="PLK55_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35430.1"
                     /translation="MNLPVTNPIVKNVPLDKIISEKLFLDLLDKKFTGYVYLIIEGKY
                     CFEEAILILNAGKIEGAIYIIEGFDIELLGKDAINYCLNCYGATNGRLNIFALTTDQI
                     KLILLFNDKIKYTLPITKNKDIPSLRNVKYNDALMYGLLKEKIVRNKTSKDILNDFNL
                     EDLLKE"
     gene            64400..64837
                     /locus_tag="PLK55_00395"
     CDS             64400..64837
                     /locus_tag="PLK55_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35431.1"
                     /translation="MSSITKLFAKFSTKILNLEDKQGENPIATLLVYKPIFIHSSDLT
                     DFLQKLRLKHLINDIIITDLDGIVIGSTNKDIKEGFKSAALYNYINSEIKDLSIILIE
                     ANGWQIIFKNNEKVYFIKANDGLSRIEVLTIVKEIENFIKCIV"
     gene            64879..65679
                     /gene="minD"
                     /locus_tag="PLK55_00400"
     CDS             64879..65679
                     /gene="minD"
                     /locus_tag="PLK55_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HOZ35432.1"
                     /translation="MGEIFAIVSGKGGVGKTTLTGNLGIALSELGYSVLLIDADLAMA
                     NLSLLMGLKTPPITIHDVLLGSAPATDAIYDGPKGTKILPAGLSLSFYTRVDIERLVA
                     VTNDLKSKFDFILIDSPAGIEKNVIAAMSASSQILLITEPTAPSVADAFKAKIVAQRL
                     NQRVFGVIINKHSNTKGEISENEIMKMLELPSYGKIPYSDDIRESFLLKKIKPLLIHN
                     PNCSAANSFRAIARKITGKETEVINTEKKKGLFSGFFEALGKMFKKKK"
     gene            65732..65962
                     /locus_tag="PLK55_00405"
     CDS             65732..65962
                     /locus_tag="PLK55_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413455.1"
                     /note="Enables 3` processing of polyadenylated mRNAs and
                     tRNA precursors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small nuclear ribonucleoprotein"
                     /protein_id="HOZ35433.1"
                     /translation="MNEIKISRPFDMLNNSLNKQVLITLKGNVTIRGKLKAFDVHLNV
                     VLEDAEEIENGKPKAKYIEVFLRGDNVILVTV"
     gene            66011..67048
                     /locus_tag="PLK55_00410"
     CDS             66011..67048
                     /locus_tag="PLK55_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35434.1"
                     /translation="MSNIILALYFGSKLAENIASDSAISNIFNYNTLIILLVVVFVVL
                     IISIFVFLIIPRINKTPKKYYQRYLLVRKELEKIDELYAKRRLSFENYVYTQFHYAKE
                     YEHLIVYLSQYPEYRVKLKTYKLNITKTKESIDLSILSEKERRNLDTTNYFITFLLPV
                     AEYYRKDEIYQAILDEGYSKEISDGVVKGLEKHGINFNSKEMSENRKAVDLVDSLLDK
                     KWRSEKTGVKEKITTPDKYYSSDSKKIESTDNVKEVSEVSQSNIFSSNVDSKGSLNTD
                     TTINLSDLIKEKSDKLKLPNFLRDQALGKDPKDEKDTPLSINKILKSKNNSHSVSEIN
                     DIFKNIDKKLK"
     gene            complement(67053..67202)
                     /locus_tag="PLK55_00415"
     CDS             complement(67053..67202)
                     /locus_tag="PLK55_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35435.1"
                     /translation="MSQSLVGGILIGIGIGLYIPSLPGFLSSFNLYLGLILIIIGLIL
                     LLKSK"
     gene            67284..67976
                     /locus_tag="PLK55_00420"
     CDS             67284..67976
                     /locus_tag="PLK55_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35436.1"
                     /translation="MKTKMCFGLLDVLVKGKLDKKPDTSFMSELVDYSKENKKELFLI
                     TGLDADKGESIIIQNGIDSYFSKENIFHVSEKYYDALSEIDKELKEQAKQNDSSYCDE
                     YYKVYFFNNVYKHKKEETLYIGHDIWTDAYYLSKYAAIDTILLKDTLTNNSAPNIMEI
                     KEVQVVDSCFPIIKSYLEEEKQFNYEPLHKYANKLLYKEMVGGSLFNTKQKVGSFWNR
                     KKDKKLFDRGEN"
     gene            67976..69208
                     /gene="eno"
                     /locus_tag="PLK55_00425"
     CDS             67976..69208
                     /gene="eno"
                     /locus_tag="PLK55_00425"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOZ35437.1"
                     /translation="MNIKKIKAIRIIDSRGVPTIRSFVFTDNAIGAASVPSGTSSGKR
                     EALELRDGGKAFGGKDVTKAINNVNTKISKKLIGEFILDQKRIDKIMLELDNTESKSS
                     LGANAVLSVSLACARAASQELNIPLYKYISKLNNKKEKNKTPIPFLNIINGGRHAGTD
                     LAFQEFQIVPHNKSFSENMRASCETYQTLKEIIKKKYGRSSTNVGLEGGFAPNIKDTK
                     DALNLLEDTITKSGYKKEITIGLDVAASEIYNNKKYLVNDKKYSSEEMSDYYSSLLKS
                     YNITSIEDPFSEDDKDSWPAFTKKFGNKISVIGDDLLVTNPKLIKEAIKKKQCNSLLL
                     KVNQIGTLTESIEAFNLAKKANWKVVVSHRSGETEDSFISDLSYGLGSDFIKFGAPVR
                     GERTAKYNRLLEIEEGVI"
     gene            69303..70151
                     /locus_tag="PLK55_00430"
     CDS             69303..70151
                     /locus_tag="PLK55_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35438.1"
                     /translation="MPKDMGDDFGSDFDVGGSDKKAFGGAKIKWETLLPVIIIIIVVL
                     LIIFKTNILASGLDIFGTTQGAKILILGAPSPEFRNILTDQENKDIIKESRFMTLDSI
                     DHNPEERIKDYDIIILDQSMQADKTITRRTGEAITNYVKKGGKLIVVLNSGIKRPGDT
                     SVLGWQANFGGIVPVNCYNSGGTPSCLSQRDLQGTLYVVKEDHPIVKGITQVPAVETS
                     GTIRTQTFDVSIDGTEIAYLEDSRTNKTYTGIVEEPLLMGKVIYFNFNPAISKAIVIK
                     TIQYLS"
     gene            70272..70943
                     /locus_tag="PLK55_00435"
     CDS             70272..70943
                     /locus_tag="PLK55_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35439.1"
                     /translation="MDKNITEKHVVEKALSELMSISKKRNFDEAIEMVVTLKNMSLKN
                     KDQRLEYAVNLPHPYLDKVKSLVFVKDKNLVQELKGVVDKVVLDEDISKISKKDAKKM
                     AEEYKVFLAEGPVMLTVGKHLGQVLSPRGKMPIVAPPNVNAIKTLITATVSQQKISNK
                     KNKSSIALQIKIGKKSQGVSNLSDNALVVIKSLVDRLPIGIQNLNHIYIKSTMSKSIK
                     MGDDK"
     gene            70940..71695
                     /gene="rplJ"
                     /locus_tag="PLK55_00440"
     CDS             70940..71695
                     /gene="rplJ"
                     /locus_tag="PLK55_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOZ35440.1"
                     /translation="MNHTKTWRLEQYDEISKLVSQYKIIGIADLKDFPSNLLQKVKKN
                     IVDATFKVTSKNVLKRVLEDKGHKDIVEKLPAQPILMLTNKNAFELYGAIKKSKARSK
                     AKIGMLSPIDIVVPAGDTGLPPGPALSDLKKVGIKAQVRGATIFVPDDTVIAKAGSVI
                     NSDIVSTLSKLDIKPVELMLDVVLVKEDGMIYNKDVLNIDAETIILQIATAARQAVGL
                     GVEIGYICKDTVEPMIAKAEREAKALDALVNKE"
     gene            71716..72006
                     /gene="rpl12p"
                     /locus_tag="PLK55_00445"
     CDS             71716..72006
                     /gene="rpl12p"
                     /locus_tag="PLK55_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HOZ35441.1"
                     /translation="MDYIYAVLLLHKANKEINEANLKSILTSAGVHADDAKLKGLVAT
                     LKDINIEDAIAKASVVAAAPAAAPAHETKKEVKEEHVVTEESAAEGLSALFG"
     gene            72102..72995
                     /locus_tag="PLK55_00450"
     CDS             72102..72995
                     /locus_tag="PLK55_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35442.1"
                     /translation="MDKSIRFSIIALVLVIFIFVPSFVFADGGPRPTREISVTYEGQI
                     IKDNEFYAAYFRCTKDMPTNEDLNSYIDAKPFAKLGPLLFIDGGYDVDKNCYWTVSGV
                     VGDQGTCKDSKCNFGYYGSSEFKFGVYLPDENKTFVSQDIIYMTAFHSTYKVNLLDNG
                     DIIVKDTTAFYKTNLAKYIFEFLIALIFTIIIEIIIIKLFLSKNLNKNRIIKIAILAN
                     LISITTLWAVCFIRWQDLNNGFLNWLIVGEIFVFLFELIFIYILNKKYITFWKVMLMI
                     FIANLASFILGGFLLFLIKIL"
     gene            73095..73973
                     /locus_tag="PLK55_00455"
     CDS             73095..73973
                     /locus_tag="PLK55_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOZ35443.1"
                     /translation="MEKENALIVFQNKKIRRTWFNEEWWFVAIDIVEVLTDSIDPSGY
                     LKDIRRRDESFNKGWGQIATPLSIQTAGGKQQLNCISLKGAFRLIQSIPSPKAEPFKR
                     WLAKVGYERIQEIQDPELIQKRLKELYRQKGYSDSWIEKRLRGIIVRDELTDEWKKRG
                     IKEEKDFSILTAEISKATFDIIPSEYKKIKNLKNENLRDHMDDLELLFIVLGETLTKE
                     IIIKEDPKELEKAKTIANRGGKVAGIARVAAEKELERKIISESNYLAIPEKRKRLRFK
                     NPKCKKPSYNTKSKSR"
     gene            74010..75566
                     /locus_tag="PLK55_00460"
     CDS             74010..75566
                     /locus_tag="PLK55_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOZ35444.1"
                     /translation="MSEFVVHPIVKKETIEKRLYQELLTAQAIKESTLVVAPTGLGKT
                     IVAVLLIAYTYNPKKSIILLSPTKPLIAQHKKSILKFLEIPEEKIILLTGETTQEKRK
                     AMYDGQGLVICATPQTIQYDLEKKLINPDNFNLVIFDEAHRAVGSYSYTFISQYFSED
                     VKRLGLTASPGSSKAKIQEVAENLGIKHIEIRTEQDLDVADYVNDIEVDINFLELDSL
                     SKEISKTIDNYIQHKIEILRKLGCPIPNNYSKKQIIAIQAGLFERVKRAKNNIGFIAI
                     SAIASVLKFLHAKELIETQGFFATETYLLKLKKESQEKKSKALSAIVNSSEFVEVLKL
                     LEKAITKRCAKEIALLEIVSSFLKENPKSRILVFNNYRDNANHLVEVLNQTAEIKSAR
                     FVGQASKSLSDKGLSQKNQTKIISEFREGLYNVLVCTSVGEEGLDIPSVDLVVFYDAV
                     ASEIRNIQRRGRTGRFNVGKVILLINKDTIDEKYYFVSQNKEKKMKSILKNFHKPERK
                     KKEQKKISDY"
BASE COUNT        27147 a        11074 c        12374 g        24826 t
ORIGIN      
        1 gtgtaaatac agcaaatgaa ttggttaatc aaaaatgtga taaagcaaca attcagatta
       61 ctgattgtgt aattaatgat gatggtaatg tcgtgcttac tataaaaaac accagttctc
      121 agtataatta tgatatatca gatgcttttt tagtttctat atataacgat tctggcacaa
      181 tggatataga aaaagaaata accttgtctt caggtacttg ggacggtcta gaggggggag
      241 agacaattat ggcaaatatt gagccaacaa cgtcagccct aacagaagat tctggggctt
      301 ggattaatgt gactttaagg tccacaatat gccctttagc agcaaccacc tattatgggt
      361 gccatagata agctttatat atgtttttat taataatata attaatattt ttaatattta
      421 aggaggatat aaaatgaatt ttaaaaagaa agcaatagaa ccaatagttg caacaattct
      481 tttggttgta gttgcagtta ttttagtcac cattgttctt gcatggggta agaatttcac
      541 aactggtggt ttaaatgaag caggcgctat aacaagcgat gcttgtaaag gcgcaacatt
      601 gtctttagaa agttgtgatt ggaattctga tacaaacaaa gtcgtattca cattaaagaa
      661 cacaagcgac acatacacct ttgatgttaa tggtttttac attaatgtag cagacagttc
      721 aacggttaca gaatcagatt tagataatgt ttcttcaact acagctttag gccctggtgc
      781 aagtttagtt gaagatatta cagtatcaga catagtagca acaaaagtaa aattaaacgt
      841 gcgttcaatt acttgtccaa ccacagcagt aagcgaactg gaatgtagtt aaaagaggta
      901 aaaaacatga attttaagaa gaaagcaata gaaccaatag ttgcaacaat tcttttagtt
      961 gtagttgctg taatattggt aacgatagtt cttgcatggg gtaaaaattt tacaaccggc
     1021 ggattagatg aagcaaataa cttaacaaac gatagttgtg ctggtgcaac catttcacta
     1081 caaagttgtg attggaatag tacttcagaa gttgcaacac ttttgataaa aaacacaagc
     1141 agtacctata ccatagctgc agatgattta tcctgtaatg ttacagacga ttcaaatgca
     1201 ttaaataatc atcaaactat accgactgca acaacagcag caatacttcc tggagcatca
     1261 atatcaacaa cttgtacagc atcttcatta gcagcagctg caacaaaagt tacggtgaat
     1321 gttaggtcag agacctgtcc aacaacagca gtaagcgaaa taaaaggttg ttcatagatc
     1381 aaaatagaat tgaaaaatta tgatttccaa aaagggggtg tcaccagtaa ttgcaactat
     1441 acttttagtt gttatagctt taatactggt gggcattctt ctttcttggg gtcaaaattt
     1501 tgtaaataaa aatacttctg atgcagatag cgcaattgat agaaaatgta ttggtgcagg
     1561 aatcaccatt aataattgtg actataatag cctttctgag actcttactt ttattattgt
     1621 taattcagga aaggttaatt tctctgcaga tcaaaacctt agtcttattc taattgatgc
     1681 agatactaat cttgtcaatg agcatcaaga tataattgat gggaatgcct ttaatagagg
     1741 cgattctttt agaatagaaa ttgacgagta tgtagcaaca acaccaatag atgtggaagt
     1801 aagaagtaca cagtgtgtta attattttgc aagagtttct tgtaattaat tagatttttt
     1861 ctatttttat aaactgtatt ctttcttctt tatcaacaat agccataaca aacttagcac
     1921 gtatggttgt tgcaagtctt tctgatttta tgatttctct agttgcaatc tttgtgcttt
     1981 taaaaacatc cacaaccatt tctgtgtgcg tgtgctctcc agcctttgat ttttgataaa
     2041 ctctaaaatc aaatccaaag atgtttccgt cttttaccac acaccctttg tttgttaatt
     2101 ccttaaatac aagatatttt tcagagaagt cagatatttc tttattacag gtcttttcta
     2161 gttcttttgt gcttattgtc ttgttgtttt tatcaacaat tataattttc ttcttttcta
     2221 gaaaataaaa acattcaaaa agatctaagt acatctcaag gccttttcta aatccaaagc
     2281 ctttctgagt aatttggctt tttaggttat tatcttttat aaggccgcta tttttttcaa
     2341 gcattacttt catgttttct ctttttcctt atttataaag cgtaaataaa tttctatcaa
     2401 ctccatagaa taaaccaatc ctaacaccag catcaatcca gccataagca taggaataag
     2461 acgctaaggc ccttgtaaag tcgccttttt cttctaagac tttcccatcc aaaagatagt
     2521 tatttgccat ctctaggtag ttgtctgcca tgtctttaag gaaagaatca ttttctatat
     2581 ttattttaat tgtggttaat gctttttcta gtagtcctct atacttaatg caatattttt
     2641 taacgtctgt attcatttat tccatctctt tcaatatcat ttaatgctcc acagattatt
     2701 aagctttgtg gaaggctttt gctttcgact tttttatagt ctaaaaaatc aaaagttata
     2761 atctcttggt ctttaccaga aatgccacaa agcccaacac atactgcgtt ttttagtttc
     2821 tcgccttttt ctttgtcaat ttcttcaagt atctggcacc cctcaaatag cgtcataaat
     2881 ttttgcttgt ctgtcttaat atcaagaaga caaagtgtat gcgtattatt ctttaggttt
     2941 tcaataatac aatcatagaa gcttgttggc ttgtagttac cctcaggata aacaatgctt
     3001 gtggtttttc caaacttata ctcaaataat ccagaagcac ccctataatt aaatatagaa
     3061 attccagcaa taatctgtgt tttaattttt cgcttgtttg cttcttcaat taatgtaaaa
     3121 tgagtggttg ctgaaaaggg attaccaata actagaaggc aagattcgtc tttgagatat
     3181 ttcttttctt gttcaagttc tgttctgttt aatggtatta tttttttttc taatattttt
     3241 tcgagttctg ttaagtctcc ttgtgagaaa atattagtgt aattatcaat atagacttct
     3301 gtgcattttt ttatgcactc aattgcttca agagtaagct gtttaggatt taaccccact
     3361 ccaattatgt aaaacatttt aactataaac tcctacttct atatatatct tattattaga
     3421 attattaata tactttatgg aattagatag aatctttgtt ttttctgttt taaaagaatt
     3481 tatttctttc cggttattat ttattataat atacccagaa gttattggaa aaagcaaaag
     3541 atagttattc tctaaagctt caagattatc aatcttaaga tattgtgaag taattaaaag
     3601 gtcatttatt ttttgcatct ttaataattc aatattgttt tctgtaaggc tgcttgagtt
     3661 aagtgtttgt gccattacta ttaaaagtga ggcaaagatt agtgcaaaaa ttccgtcaat
     3721 aacaaatatt tgtccattat ttgcattttg taaattccag aactttaaag ccattattaa
     3781 atatcacccc tctttgtata gtcatttcaa tctctgttat ttcgttttga taaatagtta
     3841 tttgacctgc tctaattctg cagatctttt cttttttcag ttcttcttca aacatcagaa
     3901 atttcaagtc caagatattc tgatagcaca ggctttcatg attgatttct gtaaaagcca
     3961 gataattaca ctcactaaca agcttatcaa taaataaaat ggattttaag tcatcaattt
     4021 ctttctgatt atttatttgt tctttggcat aattctcaaa aaataaaatt tcgaaatata
     4081 aaacaaagat aagacacaaa gaaaaaaaca aaacatcaag tactattgct tgtcccttgt
     4141 gattcatatt tctcccagtt tttttcatta taataattgg tttttataat ttttataatt
     4201 tcttttagat tcctgttttt ttctagtctt tctttaaact caagattgat attaataaaa
     4261 aatccaagta caataataat tattaaaaca cacaagataa gttctaaagt gattatccct
     4321 tttctattca agtataaaca atcacctcga ttgtgtaatt tttagggaaa gtaaaagttg
     4381 ttttagaata cgaggtcttc tgttcaaagc caagatattt atttttagct atgccgttag
     4441 ttattgtata tctttttata taaacatttg gcattggtct taagactatg gtttttgtta
     4501 tttcttgtag ttctaaaaga tttatttttt gttttttatt tgtgtttata tttagaataa
     4561 accattcttc tgtttgggcg tcttggtttg acagatagtt tattacttta gcattaattt
     4621 gtgcattaag cgttaactgg tcgttatttg gcatagaaag ttctttttta accatttctt
     4681 caaaattaat agttgcgcta tgcttttttg tgctttggga tttaactact acaaactcta
     4741 agttattttc aattctgcca ttaccagcta tagacaagaa aacaagcaaa aacaacagca
     4801 cccctagact taaaaagcct ttattttttg tgttcattaa atatttaatg atttttttcc
     4861 tttatttaac cagttgcttg ttttttttgt gtttttgaag attttgtttt atctattaaa
     4921 gcttttacaa aaaatctaac aaatgttttt aaatagctct atctacaata aatatattaa
     4981 tatcaaaaat cggaattgct ttgaagagaa acatatttgt taaagatttg tctgatacac
     5041 aggtcagtat ctttaaagat gagtctgtat tttattcaag ttatttgcct gaagacataa
     5101 aagcaagaga aaaagagatt caagaactaa gttataattt gcggccaatc tctgagaaca
     5161 gaaaagcacg aaatgtctta atctttgggc ctccaggaac aggtaaaact ttaatttcta
     5221 agtttgtgat aaggcagctt tctgaattta cacaaagagc aaaatcaatt tacattaatg
     5281 caattgaaga taataccaga tttgcagttt attcaaagct attaactctt tataattctc
     5341 cgcttccaag aagagggcta ggtattgatg aattaatttc aagagtaaaa gaagagatga
     5401 gtaaaactga ttttattcct gtaattgtta ttgatgaagt tgataagcta gaaaaatccg
     5461 aaatatcaac cattctttat gatctctcgc gtattgaagt atcaatgaaa tattttgctt
     5521 taatactaat aacaaaccac aaagaatttg taataacact tgacccaaga gtaaaaagtt
     5581 ctcttttcct ttctgaaata gagtttaaaa aatattctcc tcaggaatta aaagaaattt
     5641 taaaggaaag aatttcttac gggcttattc caaatgccat ttctgaagat atggtcggtt
     5701 atattactgg ctatgctgca aagaatggag gagacgccag aattgcaatc gacattctat
     5761 ataagtctgc aaaaactgca gaaaagaaag gaaacctaaa gattgacaag gaaacaatac
     5821 aggagtcaga gaagctaatt gatgcagtta agctttgtga taaattaaat tatattgaac
     5881 caattgaaaa agatattcta aatgcaattc caaaagaggg aatagagagt ggaaatctgt
     5941 atgctgcttt tccaaaagac tcagatagga ccgtaaggcg ccacctagat actttagaaa
     6001 aaatcggtct tataaaaaca aaagataaca tctctcagaa aggaaagaca aggaaaatag
     6061 aattgacttt tagtaaagaa ctattataaa tctaatgcca atatattttt atgaggataa
     6121 aacatgccac taaaaccaat taaaccaaca gggatacatc tagtaagagc tagagaaaaa
     6181 gctttaagca cacattcaag taagagaagt ctagagctta aaaaaaaaat ttcaaataaa
     6241 gacctaaaag aaatgcaagc cagaattaaa gagcttaaac aagaactaag aagtcaagag
     6301 tatagggttt ctaagtttaa aactgttaga gaactaaatt cagtaaagca aaaattactt
     6361 aaagaaaaaa taagaattca aggaaagtta cttgcggcct ttgatgatta ttattcttta
     6421 aatggagaac ttaaaagaat ttatgttgat tctttagagg tctatggtaa agacctttta
     6481 gaaagacaaa aaatgaattc tgagatttta aagatgctta acaagataac aaagcctgaa
     6541 gaaaaagata tgattttaag aattataaga aagaatataa agctttatta tcacatggat
     6601 ataaaataga taatgattta ctcattatct actcttggtg ccaaatagta tctgattgct
     6661 gcgctaccga ttgtgtagtt taattcaatt ggcatatcgt cttttagctt taaagttatt
     6721 gttgttttgg catttttaat catgttctgc aaatagtcta atggaaacat agctcttgct
     6781 ttatttccag agataacaat atctcctgct tcaacttctt gcttgaactt tcctttagag
     6841 ctttgtgttt ctaaaataaa cttttcgtct ccaactttta aagaaatatg tgaactgaaa
     6901 agtgcagcgt ccttgattcc tgaaagtaac tgatcagaac cgacctgcac agtcacatca
     6961 aactgtattt gtggaagtgg gtgttgctgg tctgagacgt caattaaagg gatagtaaag
     7021 gatctcttgg atttaccaat aaactctaac tgaatgcttg gctttccttc tttaatttca
     7081 attgataatt tatcgtctgc atttgagtgt gcaagaatct gcaaaagatt attaatatta
     7141 ataccaaacg agactttgtt tgctgattct gggccaatgt ctgcgataaa ttcttcaaat
     7201 gcagtttttg gcattacaaa atccacaagg cttatctggc ttgggtctgt tgctctaaga
     7261 taaaacttgt ctttgtctat tgtaaacagt ccttcgttaa tcaatacgcc gatagcgtca
     7321 atacatttat aaaattcttt tgcgtctttt attattaatt ttatcataat cccacctcat
     7381 atattattga aacaaaacta atttaatata tcctatatat ggtatttttc ctagtttctt
     7441 gccttttagg tctttttcct ttattggata gatattaagg cacggctttt gaattgtaat
     7501 ttcgttattt attatttcta gttcacaatc ctggtcaata tacacgtttg tcttgtggtt
     7561 gtctcccttt gttaatagga aggttccgtc ttttgcattt attttaacta tagctctgtg
     7621 tattatgtct tttccagtaa cgttagatgg ataaacaatt atactattat tattttctat
     7681 gctttcaagc aaagaaatat ttttgtctcc aatcttaata tactcaattt cttctatacc
     7741 aaatttgttt ttaaaaaagt taagttctgc aaagtcttta aggtctttgt ttgtaatatt
     7801 ttcgttaatt gttatttctt caagttttag attatttgct tttgtaagta ttacaatatc
     7861 tcctctgtgt aaattaggtt ccattgatga gctatataca atcgccatag gcatagacac
     7921 tccaagtatc catcccagaa tatcatagat tgctattgct aaaaagaaag caaggcaaat
     7981 atagcaaagc caataaatag tggatttcca gaagtcctct ggcttaccaa tccatttttc
     8041 taaagccatg tccaaataat taaatggatc cagataatac caaaaattct tttttggttg
     8101 tttctttgaa gctttatttt ttttattcat tttttagcaa cggccttttc aagatttttg
     8161 ataataccat taaacttttc ttctgcttca gctatacagt ctagtaaaac gtcttttgct
     8221 gctttgtctc ttgttctaag atatatagta aatctacctg ttaaattgtc ttcttctttt
     8281 tttgagacaa actctacgtc tttctttcct gaagcaatat caacaatcat atcaaagaaa
     8341 gaagagtctt catcagcaat cataaattcg atttcattct tgcctttatt tataagttct
     8401 atatccattt taaaagtcac cattttttgc aaattttttt attattgtgt ttccgcaatc
     8461 acatattact tccccatttg gtgaagcccc gattgccgct ttacattttt tacatattgt
     8521 tctaattact ccaagctctt ggttgtttgt tactagctta tactcgtatg gcccaacctc
     8581 cataaccttt gctttaatta tatcgccttt agaaaaacaa gcgctagttg attctacaaa
     8641 cttggtgtct atgtttttaa caaatataat tcccatgtct gcaggagata atactttatt
     8701 accagcatta acaatctcaa caactaccat gttatcctta acaacaacaa tttctccaat
     8761 caccacatct ccaacattta gtttttcttt ttttctaata ttggctagaa tggtacttaa
     8821 ttctaaatta ctcacaaaaa tcacaaatta gttcgttttt tatatctctc tacaatatat
     8881 ttatataaaa aaacatttat atattgttgg tttaaatatt cttttaggca aagatataga
     8941 aaaagcgaga acaataaaat ggtactagaa ttcatatata gcactcagag gatatcaagg
     9001 catccgagta ttattttttt tgagtcatta ttattgtcag ttttagcagt attgcttgct
     9061 aagttttttt ttcttccaga gcacctaagt tttggtatct ttatttttat tactgtgggt
     9121 ttaattccta tttttactaa acttttttca tataattctt atctttttaa ttattcctct
     9181 aatttctttc aaagacacaa gactttaatg ttacagcttt tgtatgtgtt tttgggtgtt
     9241 tttgtggcct ttacttttat tttctttgtt gttaacacag agacaagaga agaaattttt
     9301 gcaactcaat tatcagaaat tgaaaaaatt acagactctc gtagttctct aactggccag
     9361 atttcaggta ctgaattaaa aaacaataac gccttttcta atgcttttaa tcttatattt
     9421 tctaataatt taggagttgt attaagagca ggtctattat ctttattcta tggtgctggt
     9481 gcaatttatt tgattgcgtg gaatgcttca attttagcaa cagccatagc taatgatatt
     9541 cttgttaatc ttggcggtgt tgtaggtgtt agtggaattt ttgaaggtat tggtaatgcc
     9601 ttagtcacgc ttattggcta tatccctcac ggccttccgg aggttcttgc ttatatatta
     9661 ataagtcttg ctggtgcaat ctttgcaaga gatctaatga aggggttgtt ctttacagag
     9721 tttagatggg taataattaa ggacataatc cttttggtta ttgcttcaat tgttttactt
     9781 ttaattggtg cgctaataga agctagttat tttatctagt tttcttgtat ctttatatat
     9841 ttttacgtgt ataaatatat ttatgcaaga ttacaattat cttctaaggt ttcctttctc
     9901 taaacaggca aaggaatata tttcaagcaa gagaattgac ttgctatcag tggattataa
     9961 agttttagaa aaggttaaaa agtttttaat tgatgaaatt aaccaggact ataaaaatct
    10021 agagaaatat tggtttaata tttcaaagat tgatgatgag attattgcga caagctatgt
    10081 tatgatttat ccactatcta agattattat ttcaataatt gataacacgc cattaactca
    10141 aagctttgca aactattatc aaaaaagatt tatgtatttt tctaaaagag aagatttaga
    10201 aaatcttaaa gaaataatca cagatgtttg tcctcagttg ttttatgatt cttataaaga
    10261 acaatacttt atatctttga ttgacctatt gactttagat ttaggcgagg actataagct
    10321 acaatacaca aaccttaaag aaggaaacat tttctttcca agtaaagacg cgcttctaga
    10381 gtttctgtct gttgtcctaa agaaaagaat attaagaacc acagaaataa acaaaaaaga
    10441 gattccaaaa tcttttctgg agatttcaga aattgtaaaa aagcattttt tgtcaactca
    10501 gaaagccagg gaagatttgg attataggtt ttctggtaag ctttcgaatg gggatttccc
    10561 accatgcttt gataaattat ataatgatct cttgtcagga aagaagctgt cgcatatcgg
    10621 taactaccac ttggcagttt ttctagcagg cgttggttat acttatgacg acattataaa
    10681 tatttataaa aacgcaccaa actttgatga aagaattgcg ggctatcaga ttaaaaagat
    10741 tattgaaaag aaatattcta taggtaattg tgaaacttta aagtcaaatg atttgtgtgt
    10801 agcagactgt aaagtcaagc atcccttaca attattaaga aaacaaaatc taacagaaaa
    10861 aacatgaaac catcaaaaat taaaatatta agagcaaagt cgcctattca tgagagatta
    10921 atagatgaac gtggccgaag aagaattgat ggagatatgg acgttcaggg cattatcgaa
    10981 ggagatctaa cagatacctg ggacaaacca ggcagagata tttctaaata taaattacaa
    11041 ggaatattaa caagaagagg attaaataca gatattaggg ccttaaaaaa aagggaaacc
    11101 cctataaata tcatggtgtt gggcccagga gaaggagcag aagtgttatt attaaatgag
    11161 aaattaaagg gcacaaaaat aggaatagac acattaggtt taaccaataa tttgtctccc
    11221 gaggcaaaag agataattag aaaagattat tctccagaga aattaacagc agcaaatata
    11281 tttgaaaata tgaatcattt aaaaatggta agaaaatacg attacgttta ttctagattt
    11341 ggtccacttt ctcatacaca tcaaccagaa attgtaattt taaaagttgc aagcctgtta
    11401 aaacccggcg gctttgcaag attttatccg atagtattta gtacccctat ggaaataaaa
    11461 gaaattataa ttaactgcca aaaatacctt gcaaaaagag gttataagaa ggcaattaaa
    11521 tttaaagtgt ttgcagataa tttaataata attagagaaa aatgagatat tatgtttgga
    11581 tttaaaaaaa agaaaactaa caacaataaa ttattattga taattctaga tggtgttgga
    11641 atcaataagc catatccagg gaacgcagta actttagcaa acatgccaat ctttaattct
    11701 ctaagaacca actatccaaa tatgttactt ggcgcaagcg aagagtatgt tgggctccca
    11761 aaaaagcaga tgggttcttc agaagttggc cactttacta ttggtgctgg aagaattata
    11821 gattctgaaa ttgttagaat cgataaggcc ataagaactg gcgagttcta taaaaacatt
    11881 gttcttaacg cagagctaca aaagataaaa ggttttcagg cgcttcatgt tgttggctta
    11941 ttaagtgacg gcggagttca ttcttcaatc aatcatcttt ttgctttgct tgaagttatt
    12001 aacagacaca ataatctttc tcaagtttat cttcatcttt ttacagacgg tagagatgtt
    12061 gatcctaagt tttcaaagct ttttgtttct gagcttttga aaagaattgc aggaaataac
    12121 aggatacaca tagcaagcat ttgcggtaga tattatggca tggatagaga taatcgttgg
    12181 gataggcaaa agaaattata taatttaatt gttaatggta ttggcgaaag atcctctgat
    12241 cctttaagtt acatagagta ttgccattca aaagagatta ctgatgagtt tttgccacct
    12301 gtgttattta atgaaaacgg tttaatcaag aacaatgata cagtaatatt ttttaatttc
    12361 agaagcgaca gagcaagaga gtttacaaag atgttaatgg ataagtcttt tgacaagttc
    12421 cagacaaaga agatgttttt aaatttttta agctttacag agtatgactc aaagtttaaa
    12481 aacgtaaaag ttttgtttcc tccagaaaag caaaaagcag gtcttggtaa aattatttcg
    12541 gaacttggtt ataaacaatt aagaattgca gaaaccgaaa agtatgctca cgttacttat
    12601 ttcttcaatc ttggcgagga acatccttat aaaaaagaag acagaatact tgtgccttct
    12661 ccaagtgttg caacatatga tctaaagcca gagatgtctg caaaaattat tactcaaagg
    12721 cttatagata aattaaatga agactataag ttaattgttg ttaattttgc aaatggagac
    12781 atggttgggc acacaggaga tattcaggca acagtaaaag gtctagagat tgttgatcag
    12841 tgtcttgagc agataatgaa aagagttaat ctagataaca ctacagtaat aattacagca
    12901 gatcatggca actgcgacga gatgataacc tctgatcata aggtgacatg ccacagtatt
    12961 aataaagttc cgtttatatt aatttctaat aaggaacaca aactaagagc agacaatcac
    13021 gagtcatcga ttgctaatgt tgccccaaca attttaaagt tattaggaga agaagttcca
    13081 aaatatatgt ctgaagattt actagattat taaatggtga aatacatgaa aattttaagg
    13141 aaattattta gtaaaaaacc aaaatccgaa aaaatagttt tagataaaat aaaaacatta
    13201 aaaaaagaaa agccaaataa tggcaattat cattatttag gtggtaactg gatttctaaa
    13261 gaagcagggt cttatcacat aacttatagg cagaaaggga aaataattag tttatttgat
    13321 ttaagaaaaa cagagaaaag aaaagaaata aatccaaaag aatatagaga tttactagag
    13381 aaatcaatta aaaattccag aaatttaggc tgcgagatga ttgttataaa aacctggata
    13441 tttgcaaaat atccggaatt aaaagaattt gggtttttag agataaagcc tggttcagaa
    13501 gcaaagttct ctgagtttat ttctaaaaga aaaataactg aggaaaaatt attaagtaat
    13561 ccagagaata gtagataccc tctctactat cttaaaattt aatggatagt tatgaatatt
    13621 ctttctttac aaaaggacgg aaaagttatt tttattcctc agactcttga ggacctctgg
    13681 gttattaagt ctattactga tttaggggat gttgtttctg gttcttctta taggcgaata
    13741 cgcggtaacg aagcagacaa cgaatcaaca agaaaaccag tctttgtttc tattgctatt
    13801 gagaagtttg atttttctaa agagcttaat tctttaagat ttactggtaa gattattgat
    13861 agtaagcctg aagagcttgc gccccttggc gagtaccata ctcttgaaat ttctctttct
    13921 aaatcttaca ctctaaaaaa gccacatttc ttctctcatc aattggattt acttgagaac
    13981 aatagttctg ttttttcaag ttctattctt ttgattgctc ttgatgatga gaagtcaaat
    14041 atttttgagc ttacaaatat tggcataaaa gatatagcag tgattgactc aggaaagcct
    14101 ggcaaaagat attcttctga ttttgatttt tctagtttct tttctgaaat cttttcaata
    14161 gtttcaagat atgattgtca gattattatt gctggtcctg gtgcaacaag atctaagctt
    14221 tctgaattta ttaaacaaaa gaaaagttct ttaaaaattt tagaattatc aattcaaaat
    14281 actaacaagt cctcaatcaa cgagttattc tctaaagaag aagtttctag attttttgaa
    14341 aactcaataa tctacaaaga acaaaaggtt cttgatagct ttcttgaaaa tctaggaaag
    14401 aacaataata aagttgcata cggtttacaa gaggtgtcaa aagcacttga tgtcggcgct
    14461 tgcgaaagca ttatggtttc tgaaaagcta tggaagacag aacttgataa ggttcaagag
    14521 cttattagaa aagcagatat tgttaaaaca aaagtgcata ttgttgatga agagcacgaa
    14581 gtctctaagt ctttaaggtc ttttggcgga ataatctcca ctttaagata tcctttaagt
    14641 ttttaaaata agaaaaagag aaataaacac aactagtgtg ttatgtttct gtttattggt
    14701 tttcttgtag gaattgcatt aggatctact tttatcttta atgcttcttg ttcttttttt
    14761 tctttcttta ctgttctagt aatataagca gacattaagt taattgttaa tttagataat
    14821 ggaagattca tggtttttat aaattccttg tttgctttaa attctgtacc aaagtcttct
    14881 ttaaactcgt tatatattat ctttgctttt gcttttattt tatttgacat tgctttaccc
    14941 atggttaatt tctccttaat ttactgtgat tacaacctta ctattgttta tgtgtctaat
    15001 aactgcaagc ggtatagtct tagtatcaaa ctcgtctttt gctatagtgt aagctgtact
    15061 gtctttatgt acctttacta aagccggcgc cccgcaggat atttgctgtg ctctagcaga
    15121 tattaatcta gttatttcat attttgtaag ttttgaaacc atattaatct tttgtttaat
    15181 tattatagaa ctaaaataag gtccttttgt ctatataata tatagtagag ataaggttta
    15241 aatattattc tttttagatt actaagagat atagccaaaa tcccacaagt attagcctta
    15301 ttgctatgac tttatcaaat atcttgaata atgctaagat tatgattaat aaaagaaaga
    15361 aaggcgaaaa aacaatcttt aatagcaacc accagatact ctgcttcttt gtatatgtcc
    15421 ttttgatagc atcaaaagaa ttgtctgtgt cctggtcgct tggcacacag aagaaaagag
    15481 ccccaattcc aatccagata agcaaaaaga tatagaatgg ctcgcttgta acttttatga
    15541 tgcttgcgca aattaatgaa ataaagatat taaagaaaaa cggaaataaa gaaataataa
    15601 ttatcttata gtccttggag tcttcatgca caacatagcc gccagattta tcaataaact
    15661 taattttttt tatctttaca ccaaacatta agcaagcaag agcatgtgcg cattcgtgca
    15721 gaacaattcc aggaaagaaa agtagatgca ttatttcatt aagttccata aataataagt
    15781 atttcaatca atatttaaaa tattgttctg ttttgttaat gttatttttt gcgtttctgt
    15841 gatttctgtt attgtgcagt taatagaaag tgtagttcta atctcgcagg tcttctggtt
    15901 gtttttcaaa gttatcttgt tattgctttt ctctatttgc caagcactaa gtgcgccagg
    15961 ctcaatctca atctttcctt cgggcatgat ttcaaataac tcgatttctg cctttagatc
    16021 tttacagaag ccttttagtc tatagttgtc attagccgtt atatttctat tataaagatt
    16081 aatgattcca ataatgcttg cgccaagaac agagataatt cctgcaatca acaaaacata
    16141 ttctaaagta acaaaccctt tcatatttat tcaatatctg atatcttgtc aaatatctta
    16201 tctgaagttt tttcaacagt agtgtttgct tttgttgtgg ttttttgtaa ttgcgtgata
    16261 atgacaagaa ctatagcaac aattgctgct aaaagtatta tcatttctgc agagatttgt
    16321 cctttacaat cttttattag attcatagat atttctgtgg ttttctagtt tataaatcca
    16381 gacccttaag ttttttgtaa atttgaagaa atagttattt aaaacagaat atacacaaat
    16441 atattagata tgataaaatc aatagttcta gaaaactgga aaacgcacca gaacacaaaa
    16501 gtttcctttg agaaaggcac aaacataata gtaggcaaaa tcggggcagg gaaatctagc
    16561 atagttgatg caatttgtta ttctctttat ggcgtttttc catcccttca gaatagaaaa
    16621 ataactttag cagaaaccat aatgtttaag ccagtaaaaa gaaaagaagc aaagctaaga
    16681 ttagagtttg aacacgattc taaaaactat agaatagaaa gagaggtgtt tgctgagaaa
    16741 gtaaatatcg ctaagctttt ccttgaagat aaattgatag ctggcccaaa gcaaacagat
    16801 gttaatgaaa gagtttctga gattttaggt attgactatg atctttttgt taagattgtc
    16861 tatagcgagc aaaacgagat tgattatttc cttaagattg ctccaggtaa aagaaaggag
    16921 caatttgata ctctttttgg tatcaataat cttgagaaaa taaaagttaa ttctcgtgct
    16981 ttaaagtctt atcttgacac agaacttgat gccattcaaa gattgattgc acaattaaaa
    17041 agccaaattt cgtcttttaa tatttctgac acagaaaaaa gcattttaga aaaccaagaa
    17101 aagataaaac aacttttaga ttctacaagt aatcttaagt ccaaagaaga gggcctaaaa
    17161 tctaaagttg agattttaaa agtgcaaaaa gccgagtatg attctcttaa tcaagagata
    17221 aaaattttag attatagaga acaagagctc tcaaaagagc ttagtttttt agaaacgcaa
    17281 aaaacgaatt tctttttatt gtctaaagat gtgcttttag agaaaaaaca aattttacaa
    17341 aagcaattaa aagaaatcca agagcaagag accaagctaa aatcaattgc gtctcaactt
    17401 tttatttttg ctaatcagct tgaacagata gataagtccc tcgacgtctc tttagattta
    17461 tcaaaaataa atctagaaaa aggaaagctt tctgaagaaa tcaatagcac gattcagaaa
    17521 caagcaattc ttaattctca aaaatccgag aacctaaaag ccatatttga gcttgaaaag
    17581 ggcttttcta aatgcccggt atgtgattct caattatctg attttcagat ttctgaaaaa
    17641 ttaaaattaa aaaaagattt aaacgcaaaa ttaataaacg atgtccttgt gcttgaaaag
    17701 gcgcagttat ctcttaagca agaatttagt ctgcttcaga taaaagaaag aaaagcaagt
    17761 gagcagcatt tattaatgga tagaaagcaa agaatacttg aagaaaagaa gaaacttgaa
    17821 gaacaagaaa aggctcttgg taagcctcaa gacatttctg tgcttgacaa agaaatcgat
    17881 tcaataactc aagcaataga ttatcaaact aaaaaagagc aattgtcagg tattatagaa
    17941 aaacaagatt tcttttctaa aaaactttca aatataagct atgatgacaa caactatctc
    18001 tctttacttg cagatctaat aaatcaagaa gaaaagttaa acaacaacgc agtgcagata
    18061 tctttgatac aaggaataat aaaaaatcta gaaagcacaa aagcacaata tgaaaaatta
    18121 tctttacagc ttcaagaaaa gcaagcaata ataacaaaat tagaaacaaa acaaaaagat
    18181 atgacttttt ttgtcgctgc tcttgagaac agccaagagc atttaagaag ggtgctcgtt
    18241 gataacatca atcagacact tgaaataata tggccaaaag tctatcctta tggcgattat
    18301 ctttctgcaa ggcttagagc agaaaacgat tatgtcttgg aagtcttgac tcttcaaaaa
    18361 gaatggataa gagttgaagg tctgttgtct ggcggtgaaa gaacctgtgc tgcattaagc
    18421 ataagggttg cgattgcctt gtctttaaca aaaaaactag gtcttttgat tcttgatgag
    18481 ccaactcata atcttgatag taagacgatc actatgctct cctctattct agataaagag
    18541 cttccagagc tagttgagca aatctttata ataactcatg accaaaagtt attagaaact
    18601 tcaaactcaa gtaaatacat aattgaaaga gataaagaaa atgacggtgt atcgtttgta
    18661 aaagaggaat aatatgccaa taaaaagaac aacatttaaa tctcagattg aatcagttcc
    18721 tggaagaacc aaacacgaga tatttggtta taaaaaaccg cgcgagaaat ttattgataa
    18781 aactattaaa atagataaat ataaagtaaa atatgatgaa aaacacaaat atacaactgg
    18841 agaatataca gaaatacaca cacatctttg taatcccaaa ttagcaacag aatcaacacc
    18901 aagccctcat gattttcttg gctggattga cgactattta acaaccagtg gtaagaagaa
    18961 tagagcggtg gtttcttcta ttcattttaa gaatggagaa gagcttggga gagttcatgt
    19021 tctttttaaa aaagaggcgc taaataatca attaagaagc catatttatg attcttatct
    19081 ggaatttgca agaggaaatg gcagacaaat aattcctttt gaaaaattta atgcagattt
    19141 attttttatt ccagaaagtg gaaaagtaag aaagtatctt aatactatga tagaaaaaga
    19201 ttttgatttt gaaaaaagat taaaagtttc aaaaacaaag gatttacggg aaatatttgt
    19261 ggatatagag aagaaatggg gcataataat aaggccattt tctcatcctg gtcatgcctt
    19321 tgattataaa aaaggttcat tttattgatt tttcaagtta atactttaaa atacttgttt
    19381 tacatatatt atatatggca agtactgaaa tttctcaatt cactgtttct tatctaagag
    19441 atcttttcag aagctattat gcacataatc cagttacaag cactattgct cttgaaaaaa
    19501 gagaagtggg tattggtgat tttggaaaaa aaataagtgc caggcatctt gcattttcaa
    19561 ataatacaga attaaataga tttttagttg acaaaacccc gttttatatt tcttattcta
    19621 cagcctacta taaatatcca gaaaggcgac caatggaaaa taagattctt gaaggcagcg
    19681 acatcgtcta tgagtttgat gctgatgatt ttaatcttcc ttgtaaagaa gaacatgatg
    19741 tttggttttg tcctacttgt gaggccaatg gcagaggaca caaggacgct tgtccagaat
    19801 gccaaggaaa aacagaggtt attgaatgga cttgtgataa gtgtctgggg catgcaaaag
    19861 cagagacatt aagactaata gattttttag aaactgaatt taaactggat gctactagct
    19921 ttattataag tttttctggt tcaaaaggct atcatctaag aattacagat aaaagtataa
    19981 ttttattatc aaaatcttca agaatacaaa tgattaatta tattttaggt aaagatataa
    20041 gtcttgaaaa actaggcttt gaatacaaag gcaaacaatg gtattgccca aacccagata
    20101 caaataacgg ttgggcaggc aagttattag tatttttaaa agattatata tcaaatatga
    20161 cagaagcaca attaaggaat gactttggtt tttcaaaaat acgggcttct aaaattttag
    20221 agcataaaga agttatttta aataagatgt ataataatat attatggcac aatttttcaa
    20281 gcgattcaaa acaagatgcc aaattttggg aggatttatt agctttagca atttctaaac
    20341 ttagttttag aattgatcca acatcaaatg acatcttcaa gataatgaga atgccagata
    20401 ctattcatgg tggaacaggg cttctttcca gtaaaattaa ggacagagag gcactagaac
    20461 attttgatcc ttttgcagac cctgttcttt tcagaccaga caaagaaaga aacggagaag
    20521 tgacaagacc cattccaaag tttagattgc ttgagaaaaa atttgggccg tatagtctag
    20581 gagagaaaat agaactccca gagtatgcaa gcgtttattt attattaaaa ggggtattaa
    20641 aatgatggat tacaaagttg attatgatga attatctaga atttatagac tggagacaaa
    20701 gtcaccaaaa ctaacaaaac ttgatgcgtc attttataaa gaacttaaaa aatatctaac
    20761 tttagaaaaa acaaaatatc tttctgctct tgaaagtcta tctccggcgg atttaaaaaa
    20821 atttgaaatg ttaaagcaaa cagtaaataa aattagagaa atgagattaa agaaatgtct
    20881 taatctttgt ttaacatata gtagaacaaa cgatctttca gaagataact taattgattt
    20941 tgaaattgat tttgttaaag atattaaaaa gcttcttgac aagcaagcag aatacacaga
    21001 gagtctgttt ggtacaaaga caaagaaaga agaaaaacgc gtgcctttag taaaggttac
    21061 tgccctttca agtattcctt cttttattgg ttcagacatg caagaatatg ggccatttga
    21121 taaagacgca atatgcgaga ttccagaaaa tatttttttg attctttctt caaaaaatat
    21181 tgttcaaaaa atagaataat taaaatgtga aaaataatga cagaatatcc aaaaatttca
    21241 gataccgtga tttgtaaagt cacaaaggtt acagactatg gagtgtttgt agaactatta
    21301 gaatatgata atattgaagg gttcatccat gtttctcaag tttcttcaac ttgggtaaag
    21361 aatatacata accacgctaa aacaaaccag ataagagcag caaaagttct tcacatagac
    21421 aattacaaaa atcaaattga tctttctttt aatagagtga cacccgcaga agaaaaaaga
    21481 aagatttcag actatcgttt gtttaaaaga gcgcaaagcc tattatctgc aattgcaaaa
    21541 gaattaaaag tcagtgatga agaggtttgg tctgctgttg ctgagccaat tctagagaaa
    21601 gaaacaagtc tttatagtgg ttttattcat attctaaaga acggaataga cagctttcca
    21661 gagattgata aaaaatatac tataaaatta gttgatattc tttcaaaaag tataacaatt
    21721 aaagacaaga aaataatagg cactctagag atttctaatt caacagagaa cggaattgaa
    21781 attattaaaa aagcattaaa gaaaataaca gatgagcata aatatgttag cattacttat
    21841 gtttctgcag gaagatacga attaagtgtt acagcaaaag actataagac tgcttcaaaa
    21901 cagtttaatg aggttactaa taaacttgag aagtctttaa agggctcaaa cataaaaata
    21961 attcctcaag agaaatccaa agctaattaa tggtgtttaa aacatggcaa ctaaaattga
    22021 tgttcttaaa tctcaaaaac taagaaaccc agtaatgctt gtggggttac caggtattgg
    22081 tcttgttggc aagattgcaa ttgattattt aatctcagaa ttaaaaccaa aagcaactct
    22141 ttatgcaaca attatttctg atacttttcc tcccgcagtt catgcaaaag catcattatt
    22201 agaaatgatt tctgataata tttatcttta caaaggaaag tcaagggatt ttctttttgt
    22261 agcagggcca gttcagccaa acctaacaag tatctctaat agccaagagc attatgagtt
    22321 ttcagaaagt cttgctgagt ttgcaaaaaa gcaaggggtt aaagagatct atacttttgc
    22381 tggcctaaat atcggcgaga aaagactaaa ccaaaaacca agagttctag cagtatccag
    22441 cgataaagac actaaagaaa gactaaaaaa gaaaaagaca gataatatta tttttgatga
    22501 tactcaaggc gacactttga tttctggtgt tgctgggctt cttgtgggcg ttgcttatta
    22561 taatcacaag atttctggtt gctgttttat gggagaaact gatcaaaaac taatttttgg
    22621 tgatcaaggt tctgcaagga gcgttgttgg agttatttgc gctctctttg attttaaagt
    22681 agatatgaaa agaatagata ttcaagcaaa aaagatcgaa gaatcattta cagagattca
    22741 aaacaagata aaagaattac aaaagaaaaa agaagaacca gtaagttata ttaggtgatt
    22801 tttttaaatg cataggttag taaaaatcag aaaaccgcca gttaatgcag aaataaaaat
    22861 cgttaggcac agagttacaa ctgaaaaatt agattcttct aattttaata gttcttttat
    22921 tagtgaatta aagttttcaa aagatgctaa aggaaaacct ctaaaaattg gagaaggact
    22981 tcatgaacaa tttcttggaa caattatttt taaagacggc tctaagaaaa gagttgcaat
    23041 aaaaagattt aatgagcttc ttaaattaga tgatttttct gcaaagaagt atcagcacgt
    23101 tattgatgcg ctaaggcaaa tagaattaga gcacgacgtt gtttttccta acagacagat
    23161 aggaaaagca aagatgatac caaagatggc aatggtaaag attcaagaga aaggcaaaga
    23221 tccagaatgg gtattggttt ctcaagcttt tgtcaagaat ggtaaatcta aattaaaacc
    23281 taataatgac gtgattataa ataaattaaa tatttcagaa tatacttggg gtttacttaa
    23341 gcttggcgaa agtggattta tgaaagaaag ttcgcttggc cttgtggcag atttgtttgc
    23401 acaccacaaa agcgggtcat taattccaat agatatggat tttgcgcaat tgattgattt
    23461 taaaaagaga gaccgtaaca ctagagcaaa cgatatttta agagcgttat cccagcaggc
    23521 gcaaagcatt tcaaaaaata aagataatat taattcagta tataaaacat tagttataga
    23581 attattaaag cacaaaatgg atcctaaagt aaaggatctt ttgataagct atagaaaaaa
    23641 atatttaata aagtgatatt atgacaacaa aagaagaaaa agggattgta atttcaacgc
    23701 ttgacggtcc atcaaccaca aagctatctt ttgtttgcac taaaaatgac gtctttaaag
    23761 gagaatatat tcagattgat tattctcaag gttctcttat tggtatagta acagatgtca
    23821 taaaaagaaa tagatatttt gaaaattctg attctgttaa ggattttgaa aataacgaca
    23881 tagatataaa acgattattc ccaagcgacg agtgggagtt tttagaagcc attgttaagc
    23941 ctcttggcgt ttattctgtt gcaaacggct ttacaagaac aaatctcccg gtttctcctg
    24001 gacagaaggt ttttcttgca gatccaatag tgctttctaa gtttttgcat tttgataagt
    24061 ctggagtttt cttaggcgca atagagcatc cacatctcga tgttactctt aatgttgata
    24121 gacttcttca aaagcattta gcaatccttg cacagtctgg tgcaggtaaa agttatctaa
    24181 cttctgtaat tattgaagag cttttggacc gaacaaaaga gcaaggtcga attgcggtag
    24241 tattgtttga tactcatggc gagtattcgt cttttgctca aaaaccaaca gataaagaaa
    24301 agatagatta ttcttctaaa gtcacgctta tagattcaaa ggacgttaag attggttgta
    24361 ctggtctttc tgcgggtttc tattctacat taattccaga actaactgct cctcaaaaaa
    24421 taaggctttc aaaagttata acaaaattaa atgaggataa aaagcatggt gctggtcctt
    24481 attctctttc aaccattgtt aaagaaatag aaaattcagg agaagacgat aagacgttaa
    24541 agaatctaag agacaggctt cttgatcttg agcgttttag gttatttgat aagattgatt
    24601 atccttcaat taatgatatt gtttctccag gcaaactaac aatcattgat ctctcaagag
    24661 aaattgacaa aaagaaaaag caaattattc tttattattt tgctaacaaa ttatttagag
    24721 cgcgaatgga aaataattct gttccgccat ttaccttgat tgttgaagaa gcacatcagt
    24781 atgctcctga gatggcaagt cgtgagaatg ctttagcaaa aaacatacta gagacaattg
    24841 ctagagaagg tcgtaagttt ggagcaagcg tttggctaat ttctcaaagg ccagttaagc
    24901 tttctacaac aattctttct caagctaaca ctcacataat tctaagaata acaaatcctt
    24961 acgaccttaa acatatcgga gaatcaagtg aaggccttga tcaaaattcc attaatatga
    25021 taacttctct aagggttggc gaagccttaa ttgttggaga agccagtagt tatcctgttt
    25081 tttttaaagt gcgaaaaaga aaatcacaag aatctcatct tgatgtctcg ttatcgggcc
    25141 tagccaagtc ctttgaagac aaaaaagaaa aaaacaataa agagcttgat gatttaatct
    25201 aataaaatat atttattaag tatttggttt taattattat tatgtatttt atgtcctgga
    25261 acgagcttga ggggttgtct tttgagctta ttaaaaaaat taaagaaaag aacaaggata
    25321 agcaaataga tattgttatt tgtgtaagtc gtggtggcct tttccttggc aagattcttt
    25381 ctgaaatgct taataagcca ctagctgtaa tttctgcaaa atatctggga gacaaatata
    25441 cggtaggcac aatttcttca atctatccag taaaaggcag agtgctattg gttgatgatg
    25501 ttatggaacc tgtagttcaa gatattataa aagtcataaa acaaaataag gcagtaacgt
    25561 ctgtagagct tgcttgttta ttttatagaa agaaaaacaa aaatttcgtt ccagattttt
    25621 caattaacga aatacataat gagatttgga taactttccc atatcaaacc gaatgcttaa
    25681 aagagcatag ctattaatat gaatattgca atatttactg ataattttta tcctcagatt
    25741 aatggagtag ttactgcagt tctaaatctt gctaaaggcc ttgcagataa aggtcacaag
    25801 atctattttg ttgccccaaa gtataaaggc gaaaaagaat ttacttatcc aaacattaca
    25861 gtaagatatg ttgcaagtaa gcctgccggg tttatctata aaggcctaaa gattgtttct
    25921 ccaataagtc ggagaacaaa gaagtttatt aaagacaata acatagatgt tgtttatttt
    25981 gtaacgcctt ggacaattgc agcaaaaggg ataattattg ctaagcaatt aaagcttcca
    26041 attgtgggaa cctaccatac ctttatttgt gagaaggaat gtttaaaaca cctaaaacta
    26101 aattataatt ttgtaataaa gtttgcttgg aaatacacaa atctttatta taatacttgt
    26161 cagattgtaa cttgtccctc tgcgcttaca aaagaattat tgcttaaaaa caaatgtaaa
    26221 agaccagtaa aagtaatatc taatggcctt gatacatcct cttctccaaa atcagaaatt
    26281 caaaaaataa aaaagaagat taattctaaa gggaagacct tgttgtttgt tggtagactt
    26341 gcttacgaga agaatctttt atatcttctt gattgcttta aagaaataca taataaaaga
    26401 ccaaaaacaa aacttgtatt aatcggcgat ggtccacaga agaaagaaat tctaaaaaag
    26461 ataaaagaac taaaattaaa ggattcagta aagctacttg gcgcagtaaa atatcaagac
    26521 ttgatgacaa aaggctatta cggtgcttgt gatatatttg taaccgcttc agaaattgaa
    26581 acctttggct tgtctgtcct tgaggcgcag gcaaacgggc tagtttgtgt tggcgtaaat
    26641 aaaagtggaa ttaaaaattt aatcaaaagt aattataatg gtttcttagt taattcaaat
    26701 gataaaaatg ctttttctca agcagcaata aaactaatag ataataaaaa actatattct
    26761 aaatttaaaa aaaataataa gaagttgctt gaaaaacaca agatggaaaa tgttatttca
    26821 gaatatgaaa agacgtttaa gcaagcaata gagatgtata aatcttctta atttatattt
    26881 attttctttc tatattatat aagtgagtaa tattatggat gaatcacaag ttctattatt
    26941 aaagaaaaaa cttaaagaaa ttaagactta caaaggcgat ggtacacaac taataagcct
    27001 gtatattcct ccgaatgcgg ataggtcatc tgttactaag caattaactg atgaaatctc
    27061 acaaagctca aacattaagt ctcaacagac tagaaaaaat gttcaagcag ctctaagaag
    27121 acttacaaat tatttaagac aaatagattt taaactccca gaaaccggtc ttgttctgtt
    27181 ttctggtaat gtttctgaaa atcctgcaaa aaacgacgtt gttcttttaa ccgttatccc
    27241 cccaaaaccg ctaactacaa aactatattg gtgtgattct gagtttcata tcgcgccttt
    27301 agaagatatg tctttaactg acgaagttta tggcctagtt actattgata agcgagaagc
    27361 cactgttgca gttttaacag gcaaatctta tcacatagtt gttcatgaga ctagtggcgt
    27421 tcctggtaag actagagcag gtgggcagtc ttctgtaaga tttgaaagac taagagaaaa
    27481 ggctgcgcag gatttctata gacgagttgg ggaaagaatg agtataagta tggttgatat
    27541 tccaaaacta aaaggaataa ttgttggcgg gccaggaatg actaaaaatg agttcttaga
    27601 agaaagcgaa ttggattaca gattaaaaaa tatgataatg ggtacagttg atacaagcta
    27661 tacagacgaa tcgggaataa aagaaatgct tgataaagct ggagagcttt taaaagatgc
    27721 aagtataatt aaagaaagac aactgataaa taagtttata gaaaaggttg caacaaatgg
    27781 tcttgcaacc tatggttata atgaagttat tgaaagtatt atgcaaggaa aggctgaaac
    27841 agttattgtt tctgaagggt tagaatggag tgtacataaa tatcagtgca catcttgtgg
    27901 gcagtttacc cctaaagtta ttagagataa gaataagcaa gtaactgtgc caacaacttg
    27961 tcaatattgc gaatcttcaa caaatgaaga agaggtagag attgcagatc tttatgaata
    28021 tcttgttgat ttatcgtaca attttggcac aaaagtggtt ttaatttcta tagagacgcc
    28081 agaaggaaat caatttctaa atacctttgg tggtcttggt tcgtttttaa gatataaata
    28141 agtaaaatta tggatttcaa atatataaca acaacaaaag gtgttggtgg aagaatcaag
    28201 caattctgtg aggattttgt tgttgaggag ataggctata cttattctac aaacctaact
    28261 tatcttccag acaagaaagt tccagaacag gattggcaaa aaatctttga agaaaagaaa
    28321 gacaacgacc agcttcttgt agatatggaa aagttcaatc tttcaacaac tacagcaatt
    28381 aacgagatgt caagattttt aagaacaagt aaaagtagat ttggctatgc tggcttaaaa
    28441 gacaaaagag ccttaacagt tcagaggatt tctatttttg aaccaaacat ggaaaggctt
    28501 tcaaagtttt attttaagac aattaagatt tataatccgg tttggtcaaa gaacagagta
    28561 gatattggag atttaaaaca aaatcgtttt acaataactt ttagaaaaat aaatggttat
    28621 tccaaagaag agttagaaag attattttta gattttaaaa aacagataga aaaaaccggg
    28681 cttatcaatt actttggtga gcagaggttt ggaggagtta gagatataac tcacagagtt
    28741 ggtaaattaa ttcttcaaaa taactataaa gatgcagtaa tgctctattt aacagagtcc
    28801 acggatttag aaattccaga acttcaaaaa gcaagacgcg aattaaaaga agataataat
    28861 ttcaaaaaac acgcttatga tttccctagt ggaacaggct atgagagagt aatgttaaac
    28921 tacttagcaa acaatcctga caactatctc ggagctttca aagctttacc aaaagctatt
    28981 cagtatcttt ttattcacgc atatcaatca tatcttttta atgaaataat aaacgagaga
    29041 ataaaaagag gttacggaat tgaaaagatt gaaggcgaca caataattaa tgatgaagtt
    29101 gcaattcctc tctttggctt ttcttcaaaa ttctcagaag gtcttgctgg agttattgag
    29161 cacgaaatct tggacagaga aggggttacg attgatgcct tctttaataa agaccattca
    29221 gtcttttcca gcaaaggtga ttatcgccca ataaggattc cagtttatga tttgcaactt
    29281 ttagaaatca aagaagatga gaaaaaccaa gaagaacttc ctggttcttt gtctttgacc
    29341 ttatccttta ctcttgacaa aggcaactac gccacaaatc ttgctagaga acttattaag
    29401 cctgcgccag aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaggca
    29521 actacgccac aaatcttgct agagaactta ttaagcctgc gccagaagat aatcgttggt
    29581 gttagttttt aaaaatcaga tatttttgtt ttttcttttt tgtcttgata tgcttcagga
    29641 atctcaatct ttaattcttt taacgcctca agcccaattc ttccaatcgt ttgtaaaaac
    29701 ttttctgggt tcttctttat gtctccaatt cctgtgattc ctgaggagaa aagctttcga
    29761 gctcttactc ttccaatatt ctttagttct acaaaaggca aaagttcttt tttgattcca
    29821 tattttagtc ttagttctaa atctgagtat tccttgtaaa gctttaggct tttcttagtg
    29881 gtttttatta gttcttgtgt gcagtggttt atccagatag ccttgtttat aaattcttga
    29941 acttgcccag gagttgtgcc gtacttttca atgatatcgt tttcagatat ctcgtttatc
    30001 cagtctttta gcatttggga aagtgtaatt ttttctaaaa gattaaggtc ttcatagtca
    30061 aaataaatct ttggttttaa atattctaaa acctcaaaca catcgtcttc ttttcctttc
    30121 ttgactttaa gatacggata gcattcgcta gtattagaaa tcacaaacaa tctttccagt
    30181 tcttcaatgt cttttagttt ttcttttatt tccaaatcct taaggatgtt gttagctgaa
    30241 agggggtcaa tataaagcag gcaaactttc tttccaattt ctgtaagctt aatttcttct
    30301 tttgcctttt caataaaccc aaagtcgtta aattcttcaa gtatctctaa aacgttttct
    30361 ttaatctcct ggtctccacc gaacacaaaa tggtaaaatg ttttttccat gtatgcgtgt
    30421 atttcttttg tggttttaag gttattaata agaataattg acaatagctg tgttctaaga
    30481 ttatttattt ttgataattg ggattcgata tctgttggtg gggcaataac atatgagtta
    30541 tatactcttt gcacatctgc ttctttatta actataacaa ttgcttcgcc tttgctgtca
    30601 tattttggtc ttcctgctct tccagtcatc tgatgaaatt cgttaatggg tattaaaacc
    30661 attgagccgt ttgagaagcg gtaaactgtt gtaataacaa ctctaaaagc tggtagatta
    30721 atccctgctg caagtgttgg tgttgcaaat ataattttta tttttccgtt tctaaattct
    30781 tcttcaataa tttgtctttg tttgtaaaca agcccagcat tatgaaacgc tgttccgcaa
    30841 agaaggtcag agtaaagaga aagacattgc ttggttggtg tttcaagtgc gtctcctgct
    30901 gttcttgcaa tttttcgcag ttctgtttgt tcgttttcat ttaaaaatct cttaacaagc
    30961 tctctatatt ttgttgcaaa agacatagtg ttgcttcgcg agttgcaaaa cacaataagc
    31021 tgtttattca gttttaaagt atcagcaatt atgccgccaa ggttgtcgtc ttttcctgtt
    31081 gcttcaattt ctatttttct ctcatcaaag aaaacactat ttccatagtt aattccaata
    31141 tcaagtggta ttggtctata gttactagta actagttctg cattcagcca tttagcaagt
    31201 gcttgtgagt tgccgatggt ggcagaaagc cccaagaaaa taattttagg gaaattaact
    31261 ttaagctgcg tgattaatat ttcaagagta gttccacgat cgctaccaag ctcatgtatc
    31321 tcgtcaatac aaacaaatcc aatatcttta agccattctg gttcatgcct taaaactgaa
    31381 tcaaattttt caaatgttag aaataaaaca tcatagcttg agaggttttt ggaagaacta
    31441 tcaaggtctc ctgtagaaat tccaaatctc aaatcgtgct tctttgcata tttctttttg
    31501 aagtcgtgat aatgttcaaa ggttaatgct ttaagtggag agatatagat tacttttttt
    31561 tttcttctaa ccgcaaaatc taaaagaaac atctcaaaga tcgtagtttt ccctgacgca
    31621 gttggagcac aaacaagcag gttttctcca atgtgttttg ctgctttttc ttgcacaggg
    31681 tttagtgata aatatccatt agtctctaaa atctcagaaa ttagttcttt agtcattaca
    31741 taaaataata acctaaagtt ttttatttag agaagctaaa ataaacaatc aagctataga
    31801 acactagtct ttaggctttt ttgtaatcgc cccctctctt ttctttgccc annnnnnnnn
    31861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntagaacact agtctttagg cttttttgta
    31981 atcgccccct ctcttttctt tgcccaaata attcggagtc tattccgaat gctttttaaa
    32041 tcataaacag attagctatt aaaataatgc aaagctaggc cagcaacaag tgggtttttt
    32101 gcccacccaa accttgtgca tctggagctt atttataagt gttcttcttt aatgcgagaa
    32161 attctatttt tttaacttcc ccattgagag aaatatttat atagttaact aaacatacta
    32221 tatttatgga taaacaatat atagaaagaa taatacttga tcaatcagaa gatctaaaaa
    32281 aaatggaact tggttttaca agaaataaac tccaagatat agatagccac ataaaatcaa
    32341 aaataaatat tgtaataact ggtgttagac gctctggcaa atcaatatta ttattacaga
    32401 taatggataa atactataaa gacaagtttt attatctaag atttgcggac gaaagattat
    32461 cagatataca gatagaagat tatcaaaaaa tatacgaagt gtttttagca aattttaata
    32521 aaacaaatat tttctttttt gatgaaatac aaggaaaacc aaaatgggac cagtttgtta
    32581 atagattata tgaaaccggt aataaatttt atataactgg ctctaactcc gagttattaa
    32641 gtaaagagat atcaacttat ctaacaggaa ggcatctaga tatagagtta tatccttttt
    32701 cgtttagaga gtttttagac tttaaaaaaa tagatctaga ttacaggtat acaaaaaacc
    32761 taataaaaat agaaaaagaa attgtaaatt atttaaaaat aggcggtttt ccacaagtag
    32821 tagtagaaaa taatacctct ttacttgaag agatatatga ggacataata aacaaagacg
    32881 ttttattaag atataatata aacgatgttt ctgttcttaa aaaaacagca atatttttaa
    32941 tgtcaaacat agctaaagaa ttttcatata attccctaaa aaagatgttt aatttaggta
    33001 attcaaatac aattcgaaac tatgtttatt atttgtctaa tgcctatttg ttatttgaat
    33061 taaataaata cgattattct tttaaaaagc aagaaaatta ttcaaaaaag atatattgta
    33121 tagatactgg attaataaac aaaatggcat tttctttttc tgaaaatctt ggaagactat
    33181 atgaaaattg tgtttttata gagctaaaaa gaagaggcaa agatatatat ttttggaaag
    33241 ataaatctaa tagcgagatt gattttttaa tgcttgaaaa aaacaaggtt gtttctttaa
    33301 tacaagtttg ttataattta aatgatctta agacaaagga aagagaagtt aatgctttac
    33361 tttctgggct agatgaattt aagttaaagg aagggataat aataacaaaa gatttagaca
    33421 aagaagaaaa gataagaaat aagaccatta agtttattcc tttatggaaa tggttattag
    33481 agtaatttta aaataatagt aattaacatt ttaaacaaac aaaaaacaaa caaccgcctg
    33541 aaaaaccaat atatatatat atatatcttt catctattta ttttattagt taatttaaat
    33601 aggagataag tagataaata tgaaaaatca aaaattaaaa gcaatcactc caattgttac
    33661 aactattctt ttagtagtag ttgctgtaat tcttgtaaca atagtcttaa actggggcag
    33721 agtttttact actagttctt taagtaaaac ccaaggcata gagttaacaa aatctgatgc
    33781 agtttctttt gtttatccaa ctcagttaaa agatggcata atacaattta attattctcc
    33841 gccttcttct gagtcttttg gtgatatcat aattacaagt tataaagtat tatctgattc
    33901 tggcgagact ccagacacta atctttcttc aaattacact ttaaagcaag gttcaaatct
    33961 aatagatcta acaggttttt cttctttagg tctagactct tcttcaaaag tcacgcttgt
    34021 tttacaaaca actgacaaca cctatctaga cataaaaaac ataacaaatt attatgcagt
    34081 gcctgtgctt ttaccatatg ttttaacaaa ttcaggagac aaattatata ttcatccaag
    34141 tgattctcca acagccttaa cttgggcaga cgcaggccca tattgtgaaa atttagactc
    34201 taacggttat gacgattggt atcttccaag catgtcacaa ttagcagata tttggctagc
    34261 ttgccctgat caaacaaaat cgaacatttg tatgaataat aatataaaca aagataatct
    34321 tccatacaca gaaacctggg ttgattttat agcagactat tattggtcaa gtacggagtg
    34381 tggggcggac ggcgcttacg atatcgctat gaactatggg ggcatcggca ttaacggtaa
    34441 ggccaagggc ctctacgtgc gttgtgttcg tgaccagtag taaccagggg tttttgacaa
    34501 gccgtgggct tggcaaaagc catcaagaca tccagcggat gtcttgtttg tgattgtcag
    34561 gcaaagtcac aacccagcgg gttgtgactt tgatatgttt tgaaaaaaaa atttgtgaat
    34621 aaaaatggtt agttacgaaa accttggagt ttacaagtct tcttttgatc tttgtgtgta
    34681 ttttgaaaag attgttaaaa attttaataa gggcaataag tttacaattg gcgcagattt
    34741 aaaaaataaa tcgcgagaga ttgcaatgca agtggttaga gcaaatatat caagtacaaa
    34801 aataaaagaa attcaagagc taataatttt aattgaagaa caaaaattga tagtaagatt
    34861 gtgtcaagaa acaaacggct ttcctaataa aaattcatac ggatatatct caaaattatt
    34921 aacaaacctt acaacccaag ccaataactg gctattatgc tcgcaaaagc gagaacaaac
    34981 agagtaaaac ccggagttgt aaatgtgaat aaaagcagtt ttacaagcgc ggttattcat
    35041 ggtgatgaaa gacacctaaa cggtagtaag tattttaatt aagctctact ttttctaaaa
    35101 gatgaacttt tttaataaaa gatctttcac aaaggatgca cggagtatgg ggcggacaac
    35161 gcttacaata tcaatatgaa caatggaaac atcaacaaca acaataagac caataacaac
    35221 tacgtgcgtt gtgttcgtga acacgaattt acttttcaag aacttcataa agcatatatg
    35281 acttgtagaa aaaataaaag aaatacaatc aatgctttaa agtttgaagt tgatctggaa
    35341 agtaatttac aagagttggt gttttgttta aataacaaaa cctataagcc aactcgctct
    35401 gtttgttttt ctataaccga cccaaagcca cgcgaggttt ttgctgctga ttttaaagat
    35461 agggtagttc atcatcttct ttataaccgt ctaaacccaa tttatgaaaa aatatttatc
    35521 tatgatagct atgcttgtag aaaaaacaaa ggcactttaa actcagtaaa aagagcacaa
    35581 acctttacaa gaaagattac aaacaattca aaagaaaaag cctattatct acaactagat
    35641 attcataatt tttttatgag tattgataaa gatattttgt ttgggattgt aagccgcaag
    35701 ataaaagaca caagttggct ctggcttaca aaacagataa tctattacga cccaaccgag
    35761 aaatacttac agaaaggttc agattggaaa caatctggtg ttcctaaaca caaaacctta
    35821 tttaacacta aaaaaaacaa gggcttacca atagggaacc ttacaagcca gttctttgca
    35881 aatgtctatc ttaatgaatt agatcaatat attaaacata aaatgaaagt aaaatattac
    35941 ataagatatg tagatgattt tattatcttc tcaaaatcaa gaaaagaact attatctatt
    36001 aaaaaagaaa tagaaacctt tctaaaagaa aagctatctt tagttctaaa gcccctaacc
    36061 attctaaaac ccatttcctc aggcataaac tttgtaggct atatcataaa atcaaactat
    36121 cttttgccaa gaaatagagt agttaataat atatataaaa aaataaacct ttttaagaaa
    36181 gacagtattt atttagaaaa caaagctctt gtttatgatt tcaaaaattc ggtatatttt
    36241 ataaaatcta taaatagcta ttttggatat cttaaaccat ccaagcacaa aaatctccta
    36301 aaggatataa agacaaaaaa ccgtttttta aatattttat ttatttttga aaatcataag
    36361 atcaaaaata aataccagac aaattttata aatttaaaaa ttaaggccgt ttaacaagca
    36421 acgcatattc tcgtttaagc ttagctttat taaagctaag ctttataata tatttgtttc
    36481 ttatattata tactatttaa ataaaattac gattttctac aaaaatgatt atgcgaatgg
    36541 catcttttaa gaaaccaaaa tataataata atttgttaag gcatttataa gaggagataa
    36601 aaatgataaa caacaacgac ataattgagt tagatttaat aataaaagat aaaaacacta
    36661 attccgttct tgatacaacc cacgaagatg tagcaaaaga aggcaacatc tatgattcaa
    36721 aaacacaata taaaccttta gtggttgtct ttggtaaagg cgagttactt aaagccgttg
    36781 aagagaatat taaggattta gatattggaa aaacaaaaga attttctgta acaaaagata
    36841 aggcctttgg agatagagac ccaaaactaa tacagttact tccattatct gattttaaaa
    36901 aagaaaacat agatccaaag ccaggcatgt ttataaatct tggtaatcgc caagcaaagg
    36961 ttctaaatgt ttctggcgga agagtgcaaa tagatcttaa ccacgagttt gcaggtagag
    37021 atttagatta caagattact ttaaagaaag tttgtgcaaa cgacaacgat aagtcacaag
    37081 cattatttga gaagtatttc tttttcatgc caaaggataa ggcaaaagtt caagttaaag
    37141 aaaaaacttt agagttaata attcctggcg cgatgccaaa ggaaatcgaa tatttaaagt
    37201 acattttcat acagtctgtt tttgacacct gtaattttga aaacatacat tttatagaaa
    37261 attatgagaa gaaaaaacaa taaagggggc agagagattt tggaaacata taagaccggt
    37321 acaactacag ttgctatctt agcaaaggat tcaatagtgc ttgcagcaga catgcaggca
    37381 accatgggta atctgcaatc aaatactgag atgacaaaga tttataagat aaacgaccaa
    37441 ctagcagtaa caatcgcagg ttcagtcgga gacgccttag ttttagtaag gtttctaaaa
    37501 gtccaagcta gtctttttga tctagagcac aaaactccaa tgactccaaa agcttgtgca
    37561 accttacttt caaatatctt aaatgctaac aagatgattc cgtatgttac gcaatttatt
    37621 ctagctggtt cagttggtgg cttagcatta tataacattg atattgcagg aggaatagta
    37681 gaggaaaagg atttctcata ttcgggttca ggctcagaac tagctttgag tgttcttgat
    37741 agccattaca aaaaaggcct aaccacagaa gatgcagtta aacttgcaaa agacgcaata
    37801 acatcagcaa agaaaagaga catctacacc ggtggccaag gcataaaagt agtgattgta
    37861 aaaaaagacc gcatagagga actgccaata acaaaataca attaattctt tttttatttt
    37921 taattttttt tataaactaa tttctaaaaa caggaaaaaa acatggaaga cgaatttaaa
    37981 accataaaag attatataga aaaatttcta gcaaagaaaa acattaatgt gtctaaaatt
    38041 gatgctgaag gaccagagat tggcatctac acagatactc cattaatttt ctttgaagaa
    38101 aaagcaatta tcggcgagct tgctgagcaa ttaaaaaaaa gaataaacat tagatctgaa
    38161 aaaagtattc tgatggatcc agcagaagct ttaaagataa ttaaagagat tgtccctgaa
    38221 gatgcagatg tcaaaagcac cttctttgat acttctttta gcgaagtggt aattgaagca
    38281 attaaaccag gcgttgttat tggtaaacaa ggcatgatct caagagagat aataaaaaga
    38341 acaggttgga taccaagaat aataagagct ccaacagagc aatcagagat tctaaaaaga
    38401 gtaagattaa cgctattaaa gaacagctca gaaagaaaga ctttcttaaa gtcagtagct
    38461 aatacaatct atagcccgcc agtaagcaat aatgactgga taagagtaac tgctcttggt
    38521 ggttttagag aggtaggtag aagttgtatt cttctagaga ctcctgacac aaaaattctt
    38581 ttagactgtg gacttaatgc ttctgataaa aacgaagaag caccatactt tgatgtttta
    38641 agatttcctt tagatgaact tgatgcagtg atcattggcc acgcccatgt tgatcattgt
    38701 ggtttcttgc cttatctttt caaagcaggc tataacggcc cagtctattg tacagagcca
    38761 acaagggatt tgatggcatt acttcaattt gatctgattg atgttgcagt aaaaagtgga
    38821 agagaaccac catatcttga agagcatgtt aaagaagtaa tcaaacacac aataacaaga
    38881 gactacagag aagtaacaga cattgctcca ggaatcagat taacttttca cgacgcggca
    38941 cacattctag gttctgcttc tgttcactta catataggcg aaggaaaaca aaatctagta
    39001 tatactggcg atttcaagtt tggctttaca aggttattca ataatatgga tatcaattat
    39061 ccaagactag aatcaatgat aatggaaagc acatatggtg gtaaagaagt tacaccatca
    39121 agaatgcatt ccgaaacaaa ccttattgac gttattaatg agactacaga aaaaggaggg
    39181 ataactttaa ttccagtctt ctctgtaggt agagctcaag aggcaatgat tattcttgag
    39241 gaatactata gacaaggtaa attaaaggcc tcaaagattc ttgttgacgg catgacaaga
    39301 caggcttctg caattcacac agtttatcca gagtatctaa agaagaacat taagacaaga
    39361 gttctacaaa acaactctcc attcctttct gatatttttg ttacagttga tgatgcaaaa
    39421 gaaggcgagg aattaataac tccaggaaca gtaattctag cttcatctgg tatgctaact
    39481 ggtggcccat ctttaagata tttccagaga tttgcaagtg acccaaataa cacaatggtg
    39541 tttgtaggtt accaggcaag aaatacttta ggttacaaga tacagaaagg cttaaaatct
    39601 atgcctgtag ttgaagatgg aaaaaataaa aacctcgata ttaatatgag aatagagact
    39661 gttgattctt ttagtggcca ctcttacaga gcagagcttc ttaatttctt aaacatgtta
    39721 aagccaatgc caaaaaccat tttgattaat cacggagatt cttgtcaaga atttgcaaac
    39781 tatgttggca gaaaatacaa tgttgataca aaatctattc aggatttaga aagcataagg
    39841 ctaaggtaga ttactatgaa gatattaatt gcaggaattc ccggcgttgg caaatcagag
    39901 atttctaaag ttctaggtaa acaaaaaaaa tttaaagtaa taaacgacaa agattattcc
    39961 ataaagaata atctaggata ttatgaggta atagatggtt ataaggaata ttttgttggt
    40021 ttaaacgcgc taaatatttc tgttttaaaa gaactaaaat caaaagataa cattatcttt
    40081 gaaggccatt tatggtgtga attatcaaaa agcaatttaa agtattttga taagattatt
    40141 gttctaacta ctgataaaaa tacaattaga gagagactag agaaaaggag atataggctt
    40201 gttaagattg aagaaaacat attttgtcaa gaacacaaat atattcctga agagtttgat
    40261 aagaagaaca caaaatatat tgtgataaaa ctaaataata atttaaaaga gaatgtaaaa
    40321 aaaataagtg agaaaatatg gtaagagcac tatccactgc ccaaacacaa agtgttagag
    40381 agattaagaa gaatgcagat ataaaaaaag caaataagaa attatctgtt tttgttgtaa
    40441 ctttttttat tatgcttatt attggtttta ttgcctttaa agcctacacc tattaccaaa
    40501 gcacaaagtt tgattttaaa gaaacagaga aaggaattaa tttttattca aaagatatgc
    40561 cgattaaaga cgccttacag gtttctttag attctaataa agttgcaata tctaccaatt
    40621 tactaattaa ttattctggc acaacaaatc ctgttcttga gccatcaatt cttgcaacaa
    40681 caatctttgt ttttaaagac aaaaacacaa ttaatattaa caaccgactt gatgagaatt
    40741 tttcaataat aaaatgctca acaaattatg gagacaatct tacaagcgtt gatttaaata
    40801 gcacagagtg tttagcttta ttttctcaaa tagaatcaaa gctagtaatt aatactccaa
    40861 atccaagtct taaagaatca agggttgata tctatcctat ggaaaaaaca ataataatca
    40921 attctaaatc tgaaaccgat ttgctggttt caacctatct tttactaaag gcaaattatt
    40981 cagacgtaga gagcatatta ttcaaagttg aaaacataaa aaagaaatta tctaattatt
    41041 tagatagcaa ttctgttaaa gacacaaact ctacaacccc accagattac aataatcaag
    41101 atagcaattt agaagattta aataactagc ttatttgatc tatttttatt tcttttttta
    41161 tattaaagat ttgttctatt tctgtagctg agattattgg ttttttaaag ctattaaagt
    41221 cgtcataggc tattcttggg caagcagtat taatatagca gtcaacatca atacctagaa
    41281 gataattttc gttaatatag tcactaacaa gaatgtttgc gatttttccg tttttttcta
    41341 attttgtttt tatcttcttt gcaagtttta gtctgttctg tcctggctta ctactaacaa
    41401 gtattccaaa agatctacat tctaatgctc tagcgactgc agcatatctt tgccttaaaa
    41461 acttttcatc atatgctatt ttttcagatt gcccaataag tgggttaata agatatactt
    41521 cttgttttct gtgattgtaa gagacattgt taatatgaaa ccacccgtct ccaacaaaaa
    41581 caatcggatt agcattatcg caaagattag agcagtcaca gcccagtatc tggtgttcct
    41641 gtagcctttt tgttttattt gattctatta cttctatttt cgcttttagt agttcttctt
    41701 taatcacaga gatatttgaa agatattgca cagttgtaac tagtgatatt tccttaagcc
    41761 cgtgtttttt aatttctgaa acaataaaat ctatttgttc gttagtgtat tcaaagttta
    41821 atggcacata tattatcttt actgcttttg ttgtctttag cccaggcaat tgtctgtgcc
    41881 caaaatgaag taaggtttta cagccaagat cttttgcttc tctaaatttt atatcgcaag
    41941 caccatagca agggtcaaca aaaagagcag gagagtagtc ttttagttct tcaagaataa
    42001 gactagagaa ctgtttaagt ccttcaggta tctgtattcc cagaggcgaa acaatttctg
    42061 tttttgtttt ttttatttct gtaattatta tatctttaag tgacataata atatataaaa
    42121 cccaaaaact attaaaaccc aaagcataga gagtaagttt ataatatctt tgttattaat
    42181 ttatatgttg aataaaaaag aaaaaaactg atttttatgt atgtaggaat agacaaatca
    42241 aagcttgata cttataaaga aacaaaaaag aaaatactag gtctgattaa accaacagac
    42301 acacagatac ttaaagaaaa agaatttaca gacaaactta taaagaaagc aaaaggcttg
    42361 ccaggtaaac atctagatgt tgttcttgca ggttctgcag cgcgtaacac taatctaaaa
    42421 gaaacaaagg attttgatat ctttgttctt tatccaaagg acacttcaaa agaagaattt
    42481 acaaaagagg ccttagatat tggccaaaat ttttttaaag gttatttctc agagaaagtc
    42541 tattccgagc acccatatgt tcgaggtgtt attgatggct acaagataga tttagttcca
    42601 gcctacaaga tagattcaac agacgagatg atttcttcag ttgacagaac gcctttccat
    42661 caagcctata tttttaaaaa catgaaagag gcacagaagg atgaagttag gtttttaaag
    42721 tattttttaa agtatattgg tggttatggt gcagatcctc aatatcaagg gttctctggt
    42781 tatctttgtg agctattgat tctctactat ggtgatttta taaaaaccat agagcacgta
    42841 gcaaattggc aaactccagt tagactagtt cttgcttcag aagatattga acacttaggg
    42901 ccgtttgatg atgcctttgt aatgattgat ccagtagaca aaaaaagaaa tgtggcaagt
    42961 gctgttagta tgacaacgct atcaagattt attgcggcgt caagggcatt tttaactaaa
    43021 cccacaactg agtttttctc taacaataac aagccacaga cctatcatca gttagttact
    43081 tgggtaaata atgttccatt aacaacaatt gagtttgatg ttgaagggct tgtaaaagat
    43141 attgtttggt caaagctaag aaaatattta acaaagcttt ccaatcatct tgaaaaccaa
    43201 aagtttacaa ttttaaagtc aaatgtctat tatgaggaaa aatcaaaata cgcttatatc
    43261 attctaatga cagatatgct tgtgcttcct aagtttgaaa aagcaacagg gccttttgtt
    43321 agtgacatgt caaactctga gaaatttata gaaaactcaa aagcaatcta tggtccattt
    43381 gttaaggacg atagatggta ttgcataaag tctcgaaggt ttgttgagat aaaagacgtt
    43441 attgaagatt tctcaaatat taattttgat ataaaatgtg agatctttgt agagcaagat
    43501 atcattaagc tttacacaaa gaatacagat gttatgccat attttactga tttcttctat
    43561 ccaagagaaa actttttata aattgttctt ttcttattta atcccttttt cttttttata
    43621 tctaaacaac gtgtttctaa gagcaatcat agaaacttct gccatcactg ggtctaggtc
    43681 gccagttgta agttcttgtg cacttattct ttctcttttg gtttttcctt tttcaatttt
    43741 taataatttt ccttgaaatt taggcacgcc ttcaatatct gtaatactaa atttattaat
    43801 atcaacatct ccaaaataat aatctagttt tggtttagaa tatcgcgttg caaaaaacat
    43861 cgcagaataa cttttaattt ttgggtcaat gtctgctaaa accagctgaa gccttttaac
    43921 acttgtgcca ttgtatccgc tatctataac aacaacaggt cttttaagat taatattttg
    43981 tttaatccat tttttaagtt caataattgc agaagttaat ggtattttct ttgccatttt
    44041 ttttaatctt aaagaattag attctagcaa catatattct ttagggtgaa tatcagcaat
    44101 tagtttagtg tctgtgtgtt cttccgcttc tttactggat agtatttttt taaattgttc
    44161 ttttttaaat ccatattgag aacaaagctc tcttgctata aacatatatg gtaaagcatc
    44221 tctgtcaata aaaacaaact ctgcatttct tagttttgga tcttttgaaa atcttttaag
    44281 catagaaatt gtttggttaa ttatttcttt agcgttttgt ctataggttc tatattttaa
    44341 gtaaactttc tgtttatcta taaactttct attttctgtt ttaaaatctg tttttttaag
    44401 atcagattgg ctttctaatt ccgatatttg ctgtcgtttt cgtttttcta atctttcaaa
    44461 gaaatttccg gtctttgtct tacttccaaa ggtcttgaat gcttttgttt taccaattgc
    44521 cataattatt ctgtttttct ttaaaaaacg ggtttgggag gatttgaacc cccgtcagca
    44581 ggtccgaagc cagctgcact atccaggcta tgctacaaac ccatttataa aaatattctt
    44641 tctataatat ttatagtata agtaatactt taaagtattc gttttttaag ttattaagga
    44701 gtgttaacaa atggataaat gtacaagctg taacaaagaa gttacaacag actatacaca
    44761 gtttaaatgc ccatcttgtg gcaaaaataa aatcataaga tgtacaagat gtaaaaccac
    44821 atcaaagacc tatagatgta aagaatgtgg ctttattgga ccttaaaagg tgaaaattta
    44881 tgggagacat tctaacagtt tataaactat ttccagaaga aatagaagat acagagaaaa
    44941 ttcaggagtt tattaaaaac aactctccag gaacagagtt tagacttaat actgttgact
    45001 tagaaccaat aggttttggt ttatcagtag ttaaggtttc ttatgttttt tctgataaag
    45061 tagatggtct tttagataag cttgaaggct ttttaaaagg gatttctgga gtaaatgaaa
    45121 tagaggttat tgcaaccacc ctaatttagt ttttttaata aaaaagaatg gttttaaaac
    45181 tattcctttc tttcttttta gtatatgttt actaaaaaag gtcaactttc aatagaggtt
    45241 ctaataatta taattttttt tattaccttt atctatgttt ataataattt agcagagcaa
    45301 acagtttatt ctttagaagt aaataagata aaagagcaag agctaacaat tgctctttct
    45361 gttaatgagt ttttagaagc gcaaaaaaat attttagcag actcaactat tgtagattat
    45421 aatgcaacct atcgtttgcc aacaatagca atagcaagta gaaaattaac ttcctgtttt
    45481 gtagatatta atcttgacac acaacagctg attgttcaaa cagattattc taatattcac
    45541 acagatatag atattaatct ttcaagcaca gattttaata tttcgggcat tataagttgc
    45601 gggcagacaa ttgtgtgtga taaaccctca acaaaaatag agtgtagaca aatatgataa
    45661 agacaaaaaa agggcaagta atgttattag atgttttatt tgcagtagtt atagttatac
    45721 ttatgttttt cttgttatta aagtttgtag aaacagacat ctacaaaaca aatgtggaca
    45781 gcagaacaga agagctaaca agtattggtt ctatagctta tcagaagttt ttgaataatc
    45841 caaagattaa ttgtaaagtc acagattcac aaaattcttt ttatcttcca ggaaccatag
    45901 atacttctga aacaattaca aaagcagatt tgggtatacc tgcagattat aattgtcata
    45961 ctgacccaac agttgatggc tgtcaagcaa ccgccttttc tggtccatcc tacaccattc
    46021 aatttcctgt agttacttgt tccacagatt taaacaaaaa cacatatatc gattatatta
    46081 cagatattta taatccatca acacaactaa taacattaca agtatggagg gcgagttaat
    46141 gaaatcaaag gcttttatat ttagtgttac tgcgctttat tttgttatgt tgtttgtttt
    46201 atttatgggt ctgattactt atacttatca taacgcagtc tataagcaaa caacctcggt
    46261 ggttgataaa acagcaagtg attatattca aggaaatgat tctacatcta ttccaacaaa
    46321 tggctggtgt gtaagaagaa tagtctatga tgcaaacaca caaagcagta cccaatctcc
    46381 aagagaaata aaaacatatt gtgaggatta tggcacgcaa aggtttattt gaattggttt
    46441 ttattttagt gatgcttgtg ttaatgcttc cagcaacaat gcatcacagc gctattgtta
    46501 cctatagtgc ccaagctcaa gcaaacgatc ttttgttatt aacagaccaa gcaattgctg
    46561 atgcccttgc agatggctct cacagcggat gtttagcaag ttctaaccaa gtagctagtt
    46621 atgttaataa tcttcaaact gcatatcaag atataggaaa caattgtgag attttagtaa
    46681 atgtttctgg cacaaatcca tataccggcg atgcaaatgt aacttgcttt aagaacaatg
    46741 gcgataccac cacatacata aagaaaagaa tgacttttaa taaaacagtt actgcaactt
    46801 ctggtggtgg aacctgtaga gtaatagtta ttgataacct ttctagtgga gcaaaccaag
    46861 tagattataa tctttcaact taagtagtat aataatattt attttctttt ttctgttctt
    46921 ggtctttttg tgagcctggc ttatttgctc ttggcctctt tgtctctggg tcttgcctaa
    46981 aagtcatgtt ttcaatcttt aaaaagctat taaatgcttc ttgtatgcca gtgatgcctt
    47041 ttgcaaagcc tttttctcca ggagccccct caaacagaag cattctgtct gagatataat
    47101 ctaagagaag aagatcatga tcaacaacaa acgcgcattt ctcagtttta gttatgatgt
    47161 ttttaatagc tgtagaaacc acaagcctta gttcaacatc caagaacgca ctcggctcat
    47221 caatcaagta gatgtctgcg ctctttgata ggcaattagc tatagcaact ttttgtagtt
    47281 ctcctccact aagttctcca atcttttgat taaataagct ttctaaatca aattgtcttt
    47341 ttagttcaga ggataagatt tcttgatcaa tttcttttcc actaaacaag tctctaacca
    47401 acatctcttg atctaagtca atatattgtg gcttgtatga tatttttagt tttaaatcaa
    47461 tattattttc ttctgatttt tcaagaccag ctaagacttt aataaatgtg gttttcccaa
    47521 ttgcgtttgc cccaagtatt ccaatcactt cgcctctgtt aacttttgcc tcgtttgcct
    47581 caagcaaaaa gcttcctagc ttttttgtaa agctcggata ttcaaagaac aaaagtcttt
    47641 ttgttatgcc tttgtcttgt gtggagctaa aggttatggc atttgttcta atccttatat
    47701 tttcttcttt taaaaagccg tctagaaatt cgtttattcc tttctttgtg gtcttcttgt
    47761 ttgaggagat accatatgct tgtttctgtc caaagaacac atgcacaaag tcgcttaggt
    47821 aatccaagat tgcaagatcg tgttcaatca aaagcataga attttctgta gttatttttt
    47881 ctcttaggat ttttgcaatt ctaaatcttt gcttgatatc aagaaatgta gaatattcat
    47941 caaagaaata aagattagca gcctttaaaa gagtagaagc aattgcaact ctttgtagtt
    48001 ctccgccaga tatttctttt ggtgtttttt caagtagttt ttcaagttct agttctttaa
    48061 ttatttgcgc tttatatttt tcgtcttttg atattccatc taaaagctca tatatttttt
    48121 tgttataggc ttttgggatg tcttctacct tttgtatctt gtaagcaatt gttatcttgt
    48181 tatctcttaa gtcctcaaaa tgctgttgta ttgctttgcc tctaaagtat tcaattactt
    48241 tttctttgtc gccaggcaag gagtagtctc caaagttagg aacaaggttt ccggataaga
    48301 tgttaattgc tgtagttttc ccagcaccgt ttcttccaat aacgccagta ataaatccgt
    48361 tctctatagt tggcaaagaa aacaatctaa acatattttt tccatattgg tgaagagggt
    48421 tgtctagttc cattccaata ttaatgattg atattgcttt ttctgggcat ctgttctggc
    48481 atatgccgca gccagtacat aaggtttcat caattattgg aaatatgttt ccttgttcaa
    48541 caataattgt ttcttcgccc attcgtaccc ctgggcaaag tcttttacaa agataattac
    48601 agttctttgg ttgacatttg tctttatcaa caatagcaat tcgaatgttt tttgccataa
    48661 ttttaactca caattttctc atattgtttt tctgtaatct ttgcacccaa gtatttcatc
    48721 tcctcgagcg tgaaatcaaa gtgcgttata aggtctgttg tgcatttgtt tgtttttgca
    48781 ataattaaca taaatgggag atagtctctt aagattattc tcttagaaca atgtattctt
    48841 tgtttcattt tctctacaac agatttttgt agaatcttat ctttagtggt atacattctc
    48901 atgactcttg gaaactgaaa tggagtatat ccactactta tttctctttc tttcttagaa
    48961 taagcaacac cgacagtcaa gtagtcaaaa acatacttta aaagaaccca gttgctgcct
    49021 cttatccttg agtaattgat atcggcttta cttagattat caaaggcacg attaagatta
    49081 tttgcgttct tgtattgtct aggaatattc tcctcaaccc aaagcattaa caagtcccaa
    49141 ttgatattta ggctgtcaat aacattacga gtttcataaa agtcttttgg gtaaaatatt
    49201 ttctgtaaaa ccttaaacac atcctcgtct ttctttctat cgccaaggct ttcggtgtct
    49261 tctaatgtta tttttttatt aaaagataga gtgtagatat caatcattgt tgctctgaca
    49321 tcgttagcag cattttcaat aatactattt agagccacaa gatcataatc aatttcttct
    49381 ttatcgcaaa tctctttagc aaactttaga attgaaggtt tatgtggaag atcaaatttt
    49441 attttttcgc atttttcaaa gattggccgt agttttttat tctcgtaagg gtcgttagca
    49501 ataattatga ttggctgttt agatttgtca aaggtttttt caagctctgt aaaaaaacca
    49561 aagtcttctc cgcctttctt atcagcaaca gagtcaatct catcaattaa tactagtctt
    49621 gttgttccaa aaagagtgtt actagtgcat gccgcattaa gcaagttctt tgtgttttct
    49681 ttgtctctta agtcagaagc atcagttgtt agtattgccc attttttttc ttttgctaag
    49741 agattagcaa ctaaagtttt accaatacca ggggctccat agataaataa aggtttatca
    49801 tgtttctcac tccatcttct tatggtttca attgctggct tgtttccaac aatttcctca
    49861 aggctttttg gagtgtattt atcaaaaaag ctcttcataa tataatataa ctaaagaaag
    49921 tattaaaata ggtttattta gatttttttg tcttcgtttt ttgtgttttt caagacatca
    49981 gcaaagcttt gctttttctt ggatgctttt tctagtaatt tctttttctt agtttcccat
    50041 ttttttccag ttcttgtctt tgcaagtcca ccccttgctg tttcttttaa ttcatcatcc
    50101 attctgtttc caatctcatc catagcgctt gcaacttcgt caaaagggat ataagacttg
    50161 attccggata atgccatctg tgcagcaaca aaagctatgc cgatgcttgt tgcgtttctt
    50221 tttctgcaag gaacagaaac aacacctgct acaggatcac aaacaagccc aagcatattt
    50281 tttaatgcaa gtgctgcagc attttcgacc atctctgggt tagtgttgtc tttatagaga
    50341 agatgaatga ttgctcctgc tgccattgca gtagcagagc cacattctgc ttgacagccg
    50401 tgtgctgctc cagagattga tgcgttttta ataattgata gttctattgc tgcagcagtc
    50461 aagagtgcat tttgaatatc tttctctgaa caattataat cctgggaaac agccatgata
    50521 cagccaggaa ctattcctga ggagccggca gttgggcaag caatgactgt tcccatgtta
    50581 agattatttt cgctgattgc taatgcataa acgcaggcct tatgaagggt attacctagg
    50641 attctagttt tcttgtttgc catctttatg gaatcagaag aaaacataag attcttgaat
    50701 ttgtagttcc ctttaagtcc tgaagttatt cctctattaa atgtgtttat ggaattattt
    50761 agtttatttg aaatctcttt atcgcttctc ttaaaactaa ctctctcatc ttcttttaca
    50821 atttctgcaa ttgttatttt tcgttctttt gcaagtttaa caatatctct aagtatatac
    50881 tcggtcataa agatgcaacc tcgttaaatt ttattctccc gccaccaata gaagtgcctc
    50941 taaaaaatct ggtgatattt gctttgtttt ttactgcaag aaacactgta ttaggatggt
    51001 atttttcatc aatgtgatga tgccattcaa tctttatgcc tttactatac gcatattcaa
    51061 aagagaacgg gatctttggg tctgtttctt taaaccccaa aagcccagca acaagtgcgc
    51121 tctttgtcat gtgtgcgtct ccagtgtctg ctaatgagtt ataaagaaca atttttactg
    51181 ctttaatatc ttttacttta ccagatacct taaggccgat gttcattact cctgcagtat
    51241 gagagctaga aggcccgaca atctcagaag taacaaggtc aagtaagctt atgtatggca
    51301 tattaatctt tctccaattt ctattattat gataaacaaa aaatattata aatagcttgt
    51361 cttataatta cctttttaaa ataattgttg tttattctaa tagagtgaat aattatgata
    51421 tctacaaaaa atacaaatct attaactaaa agcttagaaa ctataacccc attaattcag
    51481 gaaacaaatg ttaggtttaa agatgaaggc atatatatca aagcaataga taagacacag
    51541 attcttctag tggacttttt catgtcaaaa aaggcctttg atacttatgc agttgaacca
    51601 agccttgtag gtctaaatat tctagaatta aaaaacatgc tttctcgttc atttggtgaa
    51661 gacaagttac aactaaatct taaagatcaa ggactggatg tgtatttgct aggtaaaatt
    51721 gaaagaaaat tttatctgtc ttttctagat ctttcagaac aaaacattaa tctcccagag
    51781 ataagatacg aggttgagtt taatattgct gccggtcttt taaaagaaat attaacagac
    51841 attaatcttg tagcttcgac cataattttt aagataaatg atggaaaatt aataatagaa
    51901 gcaaccggag aaaaagggaa tatcaaaaca acacttccag agataaaagt aaaatctaag
    51961 aaaaacttct ctgcaaagtt ttctttgtct tttttaagaa acatagttaa atctatggat
    52021 aatgataaag tgttattaat aaagcttgct gaagatgttc caatatatat agaatacact
    52081 ctaagtaaag attgtataat aaagttctat ttaagttcta tgttaatcta gagacaaaaa
    52141 atgaattcta ttaaaaaatt taattttaaa acaaaaaacg agtttcatca atttataata
    52201 aaacacacac gttttttagc agatttaata gatatacaaa attattcagg aatcgactat
    52261 aattttaata aagacgggct ttataaattt ttaaaagctg attttaaaaa aggggggtct
    52321 gtttttttaa tatatgacgg taaagaccca attggtgcga tttatggtta taataatgtc
    52381 tatggtgatc gcgaatttac tttaagtttc tttgcaataa aaaaagaata tcaaagaaaa
    52441 ggatttggca aaaaattatt gaaatttgca tacgacgaat tattaaatac tcaaggttat
    52501 aaaaatgtct gcatgtttag ttcttcaggc acaagagcaa taaataaaca gattagtggt
    52561 ttaaaaaata atgggaaatt aaagcctggc cctaaatcat ataaattaga tagcacacac
    52621 ggaacagata aagtttattt aataccaaaa ccaaggcaca tactaaaata gaaaaattat
    52681 gtcaagacct attagaccaa tgggcccgat gcttaagggg ggttattccg aaagattaac
    52741 taaaagagtt tcaagaaaga aaacaaatac cgtaaaacca ataaaattca acaaaccata
    52801 tctttctgat tacgatacaa gacttataaa gtttagattt gcgtttgaaa ataaatatgg
    52861 ttttaatatc tttaaaacaa aaggacaaga gcttcacgtt ttcttaccaa tgacatctat
    52921 gtctcctcaa ggttatctat taaaatcctt ttttgaacag actctgccac aagcaagagt
    52981 tacatttcta gtaactccaa agactgatgc gctagcaaaa acaaaagatt ccgaatttag
    53041 aagaaaggct ttagataatt tagaaaataa tttaagaagg tctcttaata tacatgacaa
    53101 atactttgtg tgttttgatt atattgacac aggaaaaacc caaataaaca ttatgcaagc
    53161 attaaacaaa atatataaca gagaagtaaa ctatatggcc gtaaaatcgc cagaaggtgg
    53221 ggcagtaaca actcacctaa aagaaacaga cggtagttct ataaggctat accattcaaa
    53281 agcaaaaact aaaaacataa tacaagaaca ttataacgca ggcatggaac tatccagaaa
    53341 aaaacaattc atgttaaagt tattagaata aagatttata ctaatttaat agcataagtc
    53401 ttatttattc tttttattaa ttcttctttc tttatagagt ctttaaatgc atgaagtatt
    53461 acttcccaaa ttgttttaac cggaataatt tctagatctt tagctacttc tggagaaaga
    53521 accaagtctt ctttgtttga ttcgggaatt ataacttttt ttatttttgc gttttttgca
    53581 gcattaactt tagcagtaac tccccctatt ggtaaaacgt ctcctctaat agaaagcgaa
    53641 ccagtcattg ctatatcttg cttaattgga atattttcaa gaaccgaaat aattgctgta
    53701 gcaatagaaa tagacgccga gtctccttca acattatagg tttgtaaaaa ctgcacatgt
    53761 atgtcatagt tagagatgtc tttgcctgtt aatttcttaa tgattgcaga aacattttct
    53821 actgattctt tagctatgtt tccaagttgt ccggttgcta tgattctgcc ttcgtttttt
    53881 gaagcagcag gagttatttc acactctatt ggtaaaagaa gcccagcatc gctttcattc
    53941 atgacagcta ggccattaac tctcccaaca gagtatcctg tggttttaaa gatatcatag
    54001 tctttcttaa caatcatcat cttgttaacc atttgttctt caagagtctt tgctgtgctt
    54061 tttgctttaa taactaaatc tcttgttaca agttttttgt tttcttctaa agccaagtct
    54121 cccgaagctc taataagtcc acctagttct cttagtttta atgttaggct attctttctt
    54181 gttgctctct ttcttgcttc aaagattatt tcatcaactg cttctttaga aaaatgtgga
    54241 atctttttat cttttgaaat ttcttgagca acaaattgca caattttgtt tctattctcg
    54301 gcagagtctg tcatatctat attcatatgg cattcatatc ctgaacctct gattcttgat
    54361 ctgattgcag gatgcacttt ttcaaggtcg atataatttc ctgcagctac aagtataaag
    54421 tcacatggag caggagtggt tctgaccatt gcgccgcttg acatttcgga ttgtccagta
    54481 atactaaatt ttttttcttg taatatagtt aataagtctt gttgtgattt tggtgaaagt
    54541 gcagagattt catcaataaa taatactcct ccatttgctt tgtgaatcat tccaggaact
    54601 actctcatat gtgcaggtgt tccaaggcct ccagattgta acggatcatg taaaacgtct
    54661 cctagtaaag cccctgcttt agcaccagtt gcatcaataa aaggaacttc tttcctattt
    54721 cgattatcaa taataatttt aggaataagt gctctttctt tttgcatcat ctgcatgctt
    54781 atagaagttg agaccattaa aaatccagat aaaagaagca ttgctgcaaa cacaacgtct
    54841 ggaacccatc caagtctcca aaaaacaata gaaattatta gccagattgt tggaagaaaa
    54901 aacccaatta ttcttcctgc gcccatttgt cttgctactt caatttttgt ggcatcagtt
    54961 atttttttac cgtctcctgc aggaacttct acaattttag gggtgtgaga gtcttcgtca
    55021 ttaggataac acatgatatc tgtaagcttg tttgttggca ttaattctgc aagtgccgaa
    55081 gcaatcattg atttaccggt cccaggagca ccaataagca ttatgtgtct tttttggcca
    55141 gcaacctttt ttataagatt gatagaagca tcttgcccaa taacctgatc aatcattttt
    55201 tttgggattt ctatttcgct cgtatcatta taaataagtt ctttaacatc attttttgct
    55261 tctgcctttg tctttgtttt attttgattt ttttttggca ttaattccac cattagtaaa
    55321 aaatagtatt taatatatat ttagtgttcc aaattgctta aaaacatatt tatacctata
    55381 tattattatg tctaacaaaa taaaagtgtt ttttttagga aattcggctt ctttgccaac
    55441 agcagagcga aatctatctt ctatgctaat aaattataat ggtcaaaact atttgtttga
    55501 ttgcccagag aattgtcagc aacagattat gaggtcaaaa caaagtataa tgaaaattaa
    55561 taatattttt ttatctcata tgcacgggga tcattttttt ggcattttag ggcttctttc
    55621 gagttttaaa ttaaataaca gaatagcaca acttaatata tttgttccaa aaaatgagaa
    55681 gaagaactta gaacaattaa taaaattaac aatatatgat aaatatccgc cagaatataa
    55741 gattaatatc gtagaagttg aaaaaaatat tgcttatcag gataaagaaa taaaagtaga
    55801 agcaatttct ctagaccaca ccacgcgaac aaacggctat atctttaaag taaaggacaa
    55861 ggttggtaaa tttaataaag aaaaagcact aaaactaaag attccagaag ggcctctatt
    55921 ttctcagctt cagaaaggca agagcataaa gctaaaagga aaaacaataa aaccagagat
    55981 ggtaatggat tataagttta agaagattgg caagaaaata gcatatcttt gtgataccca
    56041 agttttaaag aatattccaa aagagcttat tgatatagac ataatggtcc atgagtgcac
    56101 tttttttgtt gaagataaag ataaggcaag gcttgttaaa catgcagtgg ctgaagaggt
    56161 tccaatattt tctaaaaaag caaaagttaa acatctttac ttaactcaca tatctagtaa
    56221 atataatgat ttagaaaaaa gagaaaagca attacaaaag aaaaataaag gccaaaaaat
    56281 aaatatagtt cacggcttaa aagaaataga acttaatgat tattaggtat atttttaaac
    56341 atatctctaa ataacataaa tacaatataa cattaattgg tgattaaaga tggcaatagc
    56401 aaaaagagca gcaaaaagaa ttgctccaaa aactttggaa aaaatgagaa acagaactgc
    56461 agaaaaagaa agaatgaatg cagaaaaaga aaaaaacaaa atattaaaat taaaagaata
    56521 tagagaaaaa actcaagaag atttacaata tgaagcaaga tatggtaaac ttttaagtaa
    56581 tttaagaaag caaaagcgct caaaaattgc agataaagaa agatttactc ctaagatagg
    56641 tttatcagaa gctgagcgat caaaaaacat gagaaatata actcaagaga agattgatgc
    56701 tttaaaaaac agaataaatt ctattgaaaa tgcttggaag catttagaaa gccaaaaata
    56761 tgttcctgat aataataatc cacaaaaagg aatgattatt agatttctaa cagaagcaaa
    56821 aactaagaaa cagtggaaag agaggggttt tcaagaaggg cacatagttt cagaaaaaca
    56881 atataaagat gcattaaata gaaacagatt agcatataat accgcagtaa atgaattgaa
    56941 cagagcagaa tttttatatt ctctaaaaag acaagaaaga tggttgtctg gtaaaaatgt
    57001 aggagaattt aaagaagctc acgcaaaggc aatagatgtt ttagcacaaa gatatgaacg
    57061 cggacaaatt aatggagaaa ctttaaaaaa atttatgtct gttttagatt cttcagtaaa
    57121 tagaactctt gaacagcaca gagttttaga aaaaacaaaa ggttctgaat attttgatgc
    57181 gcactcaagg gttgctggga aagtgttatt gtctttcatg aaagaagcaa atttaaagaa
    57241 ctttaacgag tcagcaaaaa agtttgaatt aaaaatgagt gcaattgcgg gcacaataga
    57301 taaaccaaat agatcagtag aattagaatg gctttataaa agattataat tatttatatt
    57361 tccattcttt tccaggctca atcactattg gtttaatagt atacctggtg ttctcaaatt
    57421 tattttttag aatataaggg tcttgtttta tatgagcaaa agtattgtag ctcattggta
    57481 ttagatattt tggtcggatt ttttttgcaa cactaactgc gctaccaaga tctagtgttg
    57541 tttttccacc aatcggtaaa attgcaatat ctgcttttat cttgtcgtgc tcctttgtca
    57601 taaaagtgtc tccagaaaag taaatgctgt tttttttatc agcaataaga taactcaatg
    57661 gataaaaaga cgttgggtga tgtgcaggat atgcagtata atatatgtcg tttattgttt
    57721 ttgtcttata ttcgtccata gacactttat agttttctcc gcaatcaatt ttatttagta
    57781 cacttcggtg tgcaattact tttggtttat tttttttaac aataagatta gtatttgcta
    57841 tatcaaatga atcaaaatgt tcgtgactaa ttaaaatgct gtctattgaa ggtatgtttg
    57901 tcactggcat tgcgcaatca ataagtcgtt tatagctgca tttgtcgtta ttatcaaaat
    57961 aagggtcagt aagtatatta tttccattca tattaataat gaatgatgaa tgtcctaaat
    58021 atctaatctt aatccccata tatagattaa agtaagtaat atatttaaag caatcttcta
    58081 aatttagtaa tatgtcgttt atataaacat ttttatacca tatattacaa tatactatat
    58141 aaatatttat aggagaagaa gagaatggct aagaaacaca tattaattgg gatattattt
    58201 ttagttttat tgatgccagt atatgcagtc tccctaaaag actttgatat ttctattaac
    58261 gtttcttcgc cagataaagc aacaataatt gagacttgga atgtagatgc cggttctgaa
    58321 gaagaactat ctgattttaa atcagctatt ttaagaaaca gtgggaattt agcagaatta
    58381 agggaaataa atccagattt aaatccacac atttttgttg atgagattaa tattagaaat
    58441 tttgcaatat cttttgatga tttcaccaaa aaaataagat tagaatatac tatcgcggat
    58501 aaatgtctaa ttaattttct ggattatcaa gatcaaataa tttgggagtt aaacaatcat
    58561 ctatttaatc aatttataat tacaggggta tttaatattc caacaaattc acaaatatct
    58621 attactcttt atgatccact attgatagga gatgtcgtac cgactgcgaa aattactggg
    58681 cgaacaatat tgtggggtgg ctcttcatca aatgaaataa gagttcttgc aatagaaaag
    58741 aaaccaccaa agccaagttt tgtattttct aatttatttt ctaaagatta tcttaataaa
    58801 tcatatttta ctgttttatt tattttatta attatcgttt taatattatt aatttttaag
    58861 aacaaagtaa attccggaat aaaaaagttt attaccaaac attctgtaat aaagccaaga
    58921 aaacaaataa acgacattgt tgattttgat tttgttagta aaaaaaataa atgaatactt
    58981 atgaatttaa aatttcaaga atcagacgcg atagtttatg agtatctaaa ggagaacaaa
    59041 tctgcaatgc ttgaagaact attaaaaaat acaaactata atcaagactc tataaggcgc
    59101 tctatcgaaa ctcttaaatt aaatggcgta attgaagaaa acacaacaat ttcttatatt
    59161 tacgaattag acacaaatgg caaattggct ttagagaacg gctttataga agagttattc
    59221 tgcaattata taaaagataa gaaaataaaa gttactgaaa taaataatct agaaatacca
    59281 aatttaaata aagaagacat aattttggcc tttggtattg ctaaaaggaa agaactcata
    59341 gaaataattg acggcactct aaaaactaaa aaagaaagta aaacacaaat ttctaaacaa
    59401 aaagaaatgc tttcagatat ttccttaaaa aaaccaatta tagaaaattt agattttcaa
    59461 gaacttctaa aaagaaaaaa ttttatttta aaaaaagaaa agcaaaacaa agagtataag
    59521 ctattacaga ttataagcta caacattatt tcagatcaac aactttattt gacgcccgat
    59581 attttaaaat caggagaata ccgtaatatt aatttcaaag agtttgaggt tactgctttg
    59641 cctttgccaa aagagcaagg cagaataaat cctttaagac aagtaatcta tcaaataaga
    59701 gaattatttt tacaaatggg ttttaaagaa atgcaaggcc catacattga aagctgtttt
    59761 tggaatatgg attctatgtt tatttctcaa aatcatccag caagagacat tcaagacact
    59821 ttctatttag atcttttcgg agaattacct gccgaaagaa atttaactgc acagattgca
    59881 gagattcata ataatggctg gaaaaccggt tcaaaaggct atcagtatga atggaattgc
    59941 gaagaatcta aaaaattaat attaagaaca cacaccaccg caacaaccta cagaactttt
    60001 tttaatttgt cttctgaaga aaaagacaat tctaaatatt tttgcattga taaagtcttt
    60061 agaaacgaaa caatagattt tacacatctt cctgaatttc atcaagcgga aggctttatt
    60121 attggagaca acttgtctct agctgatctt ttagcattta ttaaagagtt tttttcaaaa
    60181 cttggaatag aaaaagtaaa attcaagcca acctataatc catatacgga accgagtgtt
    60241 gaagcaagtg gttatcaaga aagcacaggt aaatggatag aattaattaa tgcaggagta
    60301 ttcagaccag agtctttagc accatatggc atagaaaaaa cagttattgc ctggggcctt
    60361 ggcgttgaaa gactagcaat gttactatat caaaagccaa taaaagagat tcatggcgat
    60421 gaagaaaaca ttgattggtt aaaggaatat ataataccaa aaagagaact ataaatatgg
    60481 caacaataga attaaatttt tctgatgtaa aaaacgaatt taataaagac atttctattg
    60541 atgagtttga agaagtatta ttcaattatg gttttgaaat tgacagttat aataaagtca
    60601 cagatgaatt aagaatagaa gtaactgcag aaagagcaga cgttctttct aaagaaggat
    60661 tattaagagt tttaaaagtc tttggcggcc atggcaaacc tttaaactat tctgcaaaaa
    60721 agccaacaca cattttaaat attgataatt ctgtcaaatt agtcaggcca tatagcgttt
    60781 gtgcaattat taaaaatctc aagttaacag atgatagatt aaaacaaatt attgcagttc
    60841 aagaaaagct tcactttacg gtttttagaa aaaggactct tggggctatc ggagtctatc
    60901 cattagataa aataaaattt ccaataactt tctgtgcaaa aactccaaaa gatattagat
    60961 ttacgcctct tggtgaaaat aaggaaatgt ctggagaaga aattttaaaa gatacagaaa
    61021 ctggtaaaga atatgctcac ctgttattag agaaggataa atatcctatt tttatagata
    61081 attctggcca gattctatca atgccgccga taatcaattc agatgtcacc gggaaagtca
    61141 cagagaatac aaaagaaatt tttatagagt gttctggttt tgatttagta agattaaatc
    61201 aagttttaaa tataatgtgt tgcatgttta gcgactttgg tggagagctt cacgcaatgg
    61261 agattaattt aggaaattcc tatgccgaaa agcagattat tactccaaat ctaaatgaag
    61321 aaaaaagatt agtgacatta gcaaatgtta attctttaat aggtctaaat ctagatattg
    61381 ataaggctag tgttcttcta gaaaagatgt gttacaagac aaagaagtat ggtaaagaca
    61441 aaatagaagt ctcaattcca ccatacagaa cagacatctt acacgaagtt gatattatcg
    61501 atgacattgc aagagcatac ggctttaata atattccatt aaagatgcca aaagtattta
    61561 ccgttggcga aagattaaag gattcaataa aacaagacaa cataatacag atactagtgc
    61621 aatgtggttt aatagaagta agcccattgt ctctttcaag caaaaaagag tcttttgaaa
    61681 actttaatat ttcttttaca aaagacaagg caattgaact tggttattca aaagataagt
    61741 ctttagatat agttagccct tggcttttac caaagctatt aaaactttta acaaacaacc
    61801 agcacatgag ctatcctcaa aagattttta gctgtgaaaa agttgttatt cctgataaac
    61861 aaaaagacgt taaatcaaaa accattcttc attctgcgat tgccatagca aattccaagg
    61921 ttagttttac agaaagcgca agcattcttg taactttatg taatactctt ggttatagtt
    61981 tagaattaca aaaaaaagac tatcctttct atatttctgg gagatcagca acaataataa
    62041 taaacaataa agaagtaggg cacattggtg aaattaatcc agaagtatta aagcgctttg
    62101 attataatat tcctgttgtg gcatttgaaa tagatgttaa tgatttatag aagagataat
    62161 ctttaacatt tccttttctg cagccgcaaa tactttttct aaatttttaa tatgtttatt
    62221 ttttaagtct ttattcggat taagtagttt ttttagatta attggtttct tataaaacaa
    62281 gactgccaaa agatctaaat aggatttttc ttttttcata tattttttat gaattattct
    62341 tttttgtatt tcttttaaaa acaggtaata tcccattagt ttgatttttg taacataggc
    62401 ttttttggat tttatattct tgtaatatct attaagcgtt tcttctataa ttccgtcagg
    62461 ataaagcaca ggagaaactc ttatattttt aaatttacaa tcatagtaaa actcaagcat
    62521 taagtggtct tctgggttat ctgcagtaat tccgttttta ttaaaataat tatgaaagtg
    62581 tttgtctaag acattgtggc taataaaacc aatagcaaat gatttttcgg ctttagttct
    62641 tgctatagca tacattcttt ttccaaaggt tgtgcctttc ttatttctaa tttcataaaa
    62701 gaattcagga ttaagttttg tttgtatttt ataaaaaccg taataatccg gaaacaaaga
    62761 accagagaca agatatctca aatctagttt tttaaaaaaa gcaggtcttt ttttttctag
    62821 tatttcaaca aacccaagat gtgtaagaac cgttggcatt aattaaaacc atttagaaag
    62881 aaatgaatta tttctttctt gatattttaa tcattgatcc ggcttttagg tattcgattt
    62941 cattacctgc aacaagatca gtaaactcgt ctttatcaac ttcataagtt tgatagcttg
    63001 ccatatccat tacttgatat ttgtttccta ggtctgcaat tacttgtgcg tttcctttgt
    63061 caagtatagg aatttgaaca tcttctgaac ttggctttaa tagattcatc ttctgtccag
    63121 taaaaaccgc agttgctgtc attcttatct ttgcagcccc gtgttttcct ggctttgatt
    63181 tagtaattgt gtctacttta caaattacgt catcaattaa gatgaactgt ttttctttaa
    63241 cttgtccaag ttccataaat ttaatttcat aatctcttgc cattgatttc accatttttc
    63301 tattttaaaa aaagcgtttt tgtattaaaa actatataat taggtataaa tatattttaa
    63361 atatgttttg tttaacaaat actatattat atctatataa tttacaagaa gatgatcata
    63421 atgttaaaga tattcaaaaa aatatttaaa aaagtaccaa aagaaaatac aagaaataat
    63481 attgctgttt ttgttgacgg cccaaatatg ttaagaaaag agtttaatat agatttgtca
    63541 aaaattaaat ctcaattaca aaaagacggg tctttaaaga ttgcaagagt ttatcttgat
    63601 cagtacgcta cagataaatt aattgaggct gtagttaatc tgggttttga aagtgtaata
    63661 actgttggag atgtagatgt tgcaatgtct gtggatgcta cagaggccgc atttagtcaa
    63721 gagattgatt caattgtttt tattacaaga gactctgact atctgcctgc aattttaaaa
    63781 gcaaaaagac aaggcaagaa aacaatagtc tatcttgtcg aagaaggctc tgcagttgca
    63841 ttaaaaaata ccgttgatcg ggttgttttc ataaagtagg ttaaaaaatg aatttgccag
    63901 taacaaaccc aattgtaaag aatgttccac tagataagat tatttctgaa aaattattct
    63961 tagatttatt ggataaaaaa tttactggtt atgtttattt aataattgaa ggtaaatatt
    64021 gttttgaaga ggcgatatta attttaaatg ctggcaaaat agaaggggcc atatatataa
    64081 tcgaaggatt tgatatagaa ttgcttggca aagatgcaat taactactgt ttaaattgtt
    64141 atggtgcaac aaacgggaga ttaaatattt ttgcattaac taccgatcaa ataaaattaa
    64201 ttttgttatt taatgataaa ataaaatata ctttgcctat cacaaaaaat aaagatattc
    64261 ctagtttgag aaatgtaaaa tataatgatg cactaatgta tggtctatta aaagagaaaa
    64321 ttgtaaggaa caaaacatca aaagacatat taaatgattt taatttagag gatctattaa
    64381 aagaataaaa gtgaaaatta tgagttcaat aactaaacta tttgccaagt tttctacaaa
    64441 aatattaaat ttagaagata agcaaggcga aaatcccata gcaacactat tagtatacaa
    64501 accaatattt atccattctt cagatttgac tgatttttta cagaaattaa gattaaagca
    64561 cctgattaat gacataataa ttacagattt ggatggtata gtcataggca gtaccaacaa
    64621 agatattaaa gaggggttta aaagtgcagc gctatataat tatattaatt cagaaataaa
    64681 agacctctca attattttaa tagaggcaaa tggttggcag ataattttta aaaataatga
    64741 aaaggtttat tttataaaag caaatgatgg attatccaga attgaagttt taacaatagt
    64801 taaagaaata gagaatttta taaaatgtat tgtataaaat tatattgtat tttttaatat
    64861 attaagagag tgaaaaaaat gggagaaatt tttgcaattg tttctggcaa aggtggcgtt
    64921 ggtaaaacca ccttaactgg aaatttaggt attgcattat cagagctagg ctattctgta
    64981 ttgttaattg atgcagatct ggcaatggcc aatttaagtt tattaatggg gcttaaaact
    65041 ccaccaataa caatacatga tgtattatta ggttctgcgc ctgccaccga tgcaatttat
    65101 gatggcccga aaggcacaaa aatattgccg gcaggtctat ccctttcttt ttatacaaga
    65161 gtagatattg aaagattagt tgcagtaaca aacgatttaa aatcaaagtt tgattttata
    65221 ttaattgatt ctccagcagg aattgaaaaa aatgtaattg ccgcaatgtc tgcatcttcg
    65281 caaatacttt taatcaccga gccaacggct ccatctgttg cagatgcatt caaagcaaag
    65341 attgttgctc aaagacttaa tcaaagagtt tttggagtaa taattaataa gcacagtaat
    65401 actaaaggcg aaatttccga aaatgaaatc atgaaaatgt tggaattacc atcatatggt
    65461 aaaattccat attcagatga tataagagag tctttcttgt taaaaaagat aaagccactt
    65521 ctcatacaca atccaaattg ttcagccgca aattcattta gagcgatcgc aagaaaaata
    65581 acaggaaaag aaacagaagt tataaacacc gaaaaaaaaa aaggtttatt ttctggattt
    65641 ttcgaagctt taggaaaaat gtttaaaaag aaaaaataaa aaagaaaaaa ggaaaaaaga
    65701 agattgtttt aatatataaa ggagaaaaaa aatgaatgaa ataaagatat cgcgcccatt
    65761 tgacatgcta aacaattcct taaataaaca ggttttaata accttaaaag gaaacgtaac
    65821 gatcagaggg aaattaaaag catttgatgt gcatctaaac gttgtgttag aagatgcaga
    65881 agaaatagag aatggaaaac caaaagcaaa atacattgaa gttttcttaa gaggagataa
    65941 tgttatatta gtaacagtat aattctcttc taaattcctt ttttcttttt aatcattaaa
    66001 tgtaaaaact atgagcaata taattttagc attatatttt ggcagcaagt tggccgaaaa
    66061 tattgcgtct gattctgcta tttctaatat ttttaattat aatactctta ttattttgtt
    66121 agtggttgtt tttgtcgtat taattatatc gatttttgtt tttttaatta ttcccagaat
    66181 aaataaaact ccaaaaaaat attatcaaag atatttatta gtgaggaaag agttagaaaa
    66241 aatagatgaa ctatatgcaa agcgaagact ttcttttgaa aactacgtat atactcaatt
    66301 ccattatgct aaagaatatg aacacttgat agtatatctt tctcaatatc cagaatatag
    66361 agttaaatta aagacatata aattaaatat cacaaaaaca aaagaatcta tagatttaag
    66421 cattttatca gagaaggaga gacgaaattt agacactacg aactatttta taaccttttt
    66481 attacctgtt gcagaatatt ataggaaaga cgaaatatat caagcaatat tagacgaggg
    66541 ctattctaaa gaaatttccg atggtgtcgt aaagggatta gagaaacacg gcattaattt
    66601 taatagtaaa gaaatgtcag aaaatcgcaa agccgtggat ctggtcgata gtttacttga
    66661 taaaaaatgg cgttctgaaa aaacaggggt taaggaaaaa atcacaacac cagataaata
    66721 ttattcttca gacagtaaaa aaatagagtc gacagacaat gtaaaagaag tgtctgaggt
    66781 ttcccagagt aatatttttt caagtaacgt ggatagtaaa ggctctttaa atacagacac
    66841 aactataaat ttaagtgatt taattaaaga aaaatcagat aaattaaaat tgcctaactt
    66901 tttgcgcgat caagcgctag gcaaggaccc aaaagatgaa aaagacacac ctctatctat
    66961 aaataaaata ttaaaatcaa aaaataattc tcattctgtt tccgaaatta atgatatttt
    67021 taaaaatata gataaaaaac taaaataatt aattattttg attttagtaa tagtatcaaa
    67081 cctattatta ttaatattaa tccaagatac aaattaaaac ttgaaagaaa tccaggaagt
    67141 gacgggatat ataacccgat tccaattcct attaatattc cgcctacaag actttgactc
    67201 atattatcac caactaatta ttataaatac aataaatata tattagtttg tttttaaata
    67261 tatatactat aatgtgataa attatgaaaa caaaaatgtg ctttgggctt ttagatgtgt
    67321 tagtaaaagg aaaattagat aaaaaaccag atacttcgtt tatgagtgag ctagtagatt
    67381 attcaaaaga aaacaaaaaa gaactttttt taattactgg tcttgatgca gataaaggcg
    67441 aaagtataat tatacaaaac ggcatagact catatttctc aaaagaaaat atttttcatg
    67501 tttctgaaaa atattatgat gctttgtcag aaatagataa ggaattaaaa gaacaagcaa
    67561 agcaaaacga ttcgagttat tgtgatgaat attataaagt atattttttt aataatgttt
    67621 acaaacacaa aaaagaagag actttatata tcgggcacga tatttggaca gacgcctatt
    67681 atttatcaaa atatgcagca atagatacaa tcttgttaaa agacactttg actaataatt
    67741 ctgcacccaa tataatggaa attaaagaag tacaggttgt tgattcttgt tttccaataa
    67801 taaaaagtta tctagaagaa gaaaagcaat ttaattatga accgcttcat aaatatgcaa
    67861 acaaattact gtataaagaa atggttgggg gcagtttatt taatactaaa caaaaagtag
    67921 gtagtttttg gaatagaaaa aaagataaga aattatttga tagaggggaa aattaatgaa
    67981 tattaaaaaa ataaaggcaa taagaataat cgattccaga ggtgtgccaa caattagatc
    68041 ttttgtgttt acggataacg caataggggc ggcaagtgtt ccgtctggaa ccagtagtgg
    68101 taaaagagaa gccttagagt taagagatgg cgggaaggct tttggtggaa aagatgtaac
    68161 taaggcgatc aataatgtca atacaaaaat ttcaaagaaa cttattggag aatttattct
    68221 tgaccaaaaa agaatagata aaataatgtt agaattagac aacacagaaa gcaaatcaag
    68281 tttgggggcc aatgccgttt tgtcagtaag tcttgcttgt gcaagagcag cttctcaaga
    68341 gcttaatatt cctttatata aatatatttc aaaattaaat aataaaaaag aaaaaaacaa
    68401 aacaccaatt ccgtttttaa acataataaa tgggggaaga cacgcaggaa cggatttggc
    68461 atttcaagag tttcagatag ttcctcataa caaaagtttc tcagagaata tgagggcatc
    68521 ttgcgaaacc tatcaaacgc taaaagaaat aattaagaaa aaatatggga gaagttctac
    68581 aaatgttggc cttgaaggtg ggtttgcacc aaacataaaa gacacaaaag acgccttaaa
    68641 ccttttagaa gatactatta caaaatcagg atataaaaaa gaaataacaa ttggtcttga
    68701 tgttgctgct agtgaaattt ataataataa aaaatatctg gtaaatgaca aaaaatattc
    68761 ttcagaagag atgagtgatt attattcttc tcttttaaaa tcttataata taacttcgat
    68821 agaagatcca ttttctgaag acgataaaga ttcctggcca gcctttacaa aaaagtttgg
    68881 gaataagatc tccgtaattg gcgacgatct tttagttaca aatccaaaat tgataaaaga
    68941 agcaataaaa aagaagcaat gtaattctct tcttttaaag gtaaatcaaa ttggaacatt
    69001 aacagaaagc attgaggcct ttaatttagc aaaaaaagca aactggaaag ttgttgtttc
    69061 acaccggtct ggagaaacag aagattcttt tatttctgac ctgtcatatg gtttaggttc
    69121 agactttatt aagtttgggg ccccagtaag gggagaaaga actgcaaaat ataacagatt
    69181 attagaaatt gaagaagggg ttatataatt tgttaaagtt aagaatttac gaaaccttta
    69241 aaaacaaata ttatataaac ttatttataa tatatatatt aaaataaaaa agtgataaat
    69301 ttatgccaaa agatatggga gacgatttcg gttctgactt tgatgttgga ggttctgata
    69361 aaaaagcatt tggtggagca aagattaaat gggaaacctt gttaccagta ataataataa
    69421 taattgttgt tttactaatt atttttaaaa caaatatttt agcgagtggt ttagacattt
    69481 ttggaaccac gcaaggtgca aagattttaa ttctaggggc cccatctcct gaatttagaa
    69541 acatattaac agatcaagaa aacaaagaca ttatcaaaga atcaagattt atgactttag
    69601 atagtatcga tcacaatcct gaagaaagaa taaaggatta tgatataatt attttagatc
    69661 aatcaatgca agcagataag accataacta gaagaactgg cgaagcaatt acaaattatg
    69721 ttaaaaaagg cgggaaacta attgttgttt taaattccgg aattaaaaga ccaggtgata
    69781 cttctgttct tggttggcaa gcaaattttg ggggaatcgt tccagtaaat tgttataatt
    69841 cagggggaac accatcctgc ttaagtcaaa gagatcttca agggacttta tatgttgtca
    69901 aagaagatca tccaattgta aaaggcataa cacaagttcc agcagttgag acctctggaa
    69961 caataagaac tcaaaccttt gatgtttcaa tagatggtac cgagattgct tatttagagg
    70021 attcaagaac aaataaaaca tatacaggaa ttgttgaaga accattatta atgggtaaag
    70081 ttatttattt caatttcaat cctgcaataa gtaaagcaat tgtaattaaa acaatacaat
    70141 atctaagtta agtatagtaa cttaactttt ctttttattt tttaaaatac atttataaat
    70201 tgtattattc ataaattata tatataatat tttcccgtta atactgcttt aaggcaggag
    70261 gtgctaagat tttggataag aatataacag aaaaacatgt agttgaaaag gcgttatctg
    70321 aattaatgtc tatttcaaag aaaaggaact ttgatgaagc aatcgaaatg gttgtaacat
    70381 taaagaatat gagtcttaaa aataaagacc aaagattaga gtatgcagta aatctaccgc
    70441 acccttattt agacaaagta aagtctttag tttttgtaaa ggataaaaat cttgtacaag
    70501 aattaaaagg cgttgttgat aaggttgttt tagatgaaga tatttctaag atttctaaga
    70561 aagatgcaaa aaaaatggca gaggaatata aggttttctt ggctgaagga ccagttatgc
    70621 taaccgtcgg taagcactta ggacaggttc ttagtcctag aggaaagatg cctattgtcg
    70681 ctccaccaaa cgtgaatgca ataaaaacac taatcacagc aacagtttca caacagaaga
    70741 tatcaaataa gaaaaataag agttccattg ctctacaaat aaaaatcggg aaaaagagtc
    70801 aaggggtttc taatttatct gataatgctt tagttgtaat aaaatcatta gttgatagat
    70861 tgccaatagg tatacaaaac ttaaatcaca tctatatcaa gtcaacaatg agtaaaagca
    70921 taaaaatggg tgatgacaaa tgaatcacac aaaaacttgg agactggaac aatatgacga
    70981 aattagtaag ttagtttcac aatataaaat aattggtatt gctgatctta aagactttcc
    71041 atctaatctt ttacaaaagg ttaagaaaaa cattgttgat gcaactttta aagtaacctc
    71101 aaagaatgtt ttaaagagag tacttgaaga taaggggcac aaagatattg ttgaaaaact
    71161 accagctcaa ccaatattga tgttaacaaa taaaaatgct tttgagttat acggtgcaat
    71221 aaagaaaagt aaggctcgtt caaaagcaaa gataggaatg ttatctccaa tagatattgt
    71281 tgttccagca ggagacacag gtcttccacc aggcccagca ttatctgatt tgaaaaaggt
    71341 tggtatcaag gctcaagtaa gaggtgcaac aatttttgtt cctgatgaca cagtcatagc
    71401 taaagctggt agtgtcatta acagcgatat tgttagtact ttgtctaagc ttgatattaa
    71461 acctgtcgaa ctaatgctag acgttgtttt agtaaaggaa gacggaatga tatataataa
    71521 agatgtatta aacatcgatg cagaaaccat aatcctgcag atagcaacag cagcgcgtca
    71581 agcagttggt cttggtgttg aaatcggtta tatctgtaaa gatacggttg agccaatgat
    71641 agctaaagca gaaagagagg ctaaagcact tgatgcttta gtcaataagg aataattaaa
    71701 tgaggtgtag aaaatatgga ttatatatac gcagttttgt tattacacaa agcaaataaa
    71761 gaaataaacg aggcaaactt aaagtcaata ttaacttcag caggagttca tgcagacgat
    71821 gctaagttaa aagggcttgt agcaacctta aaggacataa atattgaaga cgcaatagca
    71881 aaggcttcag tagttgcagc agctccagca gcagcaccag ctcatgagac aaagaaagaa
    71941 gttaaagaag agcacgttgt tactgaagaa tcagcagcag aaggcctaag cgcattattt
    72001 ggttaatttt aataaccaaa taatcttttt ctttttttta ttttatttaa aaccttagaa
    72061 taaaaatcta tttataaaac tttgtttcta tctatttcct tatggataaa agcattagat
    72121 tttcaattat agcattggtt ttggttatat ttatttttgt gccaagtttt gtttttgcag
    72181 atggcggccc taggccaact agagaaatat ctgttactta tgaaggccaa ataattaaag
    72241 ataacgaatt ttatgcagca tattttaggt gtactaaaga tatgccaaca aatgaagatt
    72301 taaattcata tattgatgca aaaccatttg ctaagcttgg tccattgtta tttatagatg
    72361 ggggttatga tgttgataaa aattgttatt ggacagtaag cggtgtggtg ggcgatcaag
    72421 ggacttgtaa agattcaaaa tgtaattttg gatattatgg gtcttcagag ttcaaatttg
    72481 gagtatatct tcctgatgaa aataaaacct ttgtatcgca agacattatt tatatgactg
    72541 catttcatag tacctataaa gtaaaccttt tagataatgg agacatcatt gtaaaagaca
    72601 caaccgcatt ttataaaaca aatttagcaa aatatatttt tgaattttta atagcattaa
    72661 tttttactat aattatagaa attataataa ttaaattatt tctttcaaag aatttaaata
    72721 aaaataggat aataaagatt gccattttag ccaatcttat atctataaca acattatggg
    72781 ctgtgtgctt tattagatgg caagatttaa ataatggttt tttgaattgg ttaattgttg
    72841 gggagatatt tgtattctta tttgaattaa tctttattta tattttaaat aagaaatata
    72901 taactttttg gaaggtcatg ctaatgattt ttattgcaaa tttagctagt tttatattag
    72961 gaggttttct attattttta attaaaattt tataagcgat tctatttata aactattttt
    73021 ttagtcttgc actggacagc caccctagtt aaatatatat acatcaattt caagaaatat
    73081 attggtgtat agttatggaa aaagaaaatg cattaattgt ttttcagaat aaaaaaatta
    73141 gaagaacctg gtttaatgaa gaatggtggt ttgtagcaat agacatagta gaagtattaa
    73201 ctgattctat agatccttca ggatatttaa aagatataag aagaagagat gaatctttta
    73261 acaaagggtg ggggcaaata gccacccccc tctcaataca aacggccggt ggtaaacaac
    73321 aattaaattg tatttcactt aaaggcgctt ttcgtttaat tcaatcaatc ccctctccaa
    73381 aagctgaacc tttcaagagg tggctagcca aagtcggtta tgaaagaata caagagattc
    73441 aagatccaga acttattcaa aaaagattaa aagaactata tagacaaaaa ggatattctg
    73501 attcttggat tgaaaaaaga ttaaggggga taatagttag agatgaatta acagacgaat
    73561 ggaaaaaaag agggataaaa gaagaaaaag atttttccat tctcacagca gagatttcta
    73621 aagcaacttt tgatataatt ccaagcgagt ataaaaaaat aaaaaattta aaaaatgaaa
    73681 atctaagaga tcacatggac gatttagagt tgttgtttat agttcttgga gaaactctta
    73741 ctaaagagat tataattaag gaagatccaa aagaactgga aaaagcaaaa acaattgcaa
    73801 atcgtggagg caaagttgca ggaattgcaa gagttgctgc agaaaaagaa ttagaaagaa
    73861 aaattatttc agagagcaat tatcttgcaa ttcctgaaaa acgaaaaaga ttaagattta
    73921 agaacccaaa atgcaaaaag ccaagttaca atacaaaatc aaaatcaaga tagtttttta
    73981 aatataagaa acacaatata ttataaatta tgtcagagtt tgtagttcac ccaattgtta
    74041 agaaagaaac aatagagaaa aggctttatc aagagcttct tacagcgcaa gcaataaaag
    74101 aaagcacact tgtggttgct ccaacaggtc ttggcaaaac cattgttgca gttctattaa
    74161 tcgcttatac ttataatcca aagaaatcaa ttatcctttt gtctccaaca aaaccgttga
    74221 tcgctcagca caagaaaagc attctaaaat tcttagagat tccagaagag aaaattattc
    74281 ttttaacagg agaaacaacc caagagaaaa gaaaagcaat gtatgatggg caaggtcttg
    74341 taatctgtgc aacaccgcag actattcaat atgacctaga aaagaaatta attaatcctg
    74401 acaacttcaa tttggttatc tttgatgagg cgcatagagc cgttggcagt tattcctata
    74461 cttttatttc tcaatatttt tctgaagatg ttaaaaggct tgggctaaca gcttctcctg
    74521 gctcgagtaa agcaaagatt caagaagttg cagaaaatct aggaataaag cacatagaga
    74581 taagaacaga gcaagatctt gatgttgcag attatgttaa tgatattgaa gtagacataa
    74641 attttttaga gcttgattct ctttcaaaag aaatttcaaa aaccattgac aattatattc
    74701 aacataagat tgagatttta cgaaagcttg gctgtccaat tccaaacaat tactcaaaaa
    74761 agcaaatcat cgcaattcaa gctggtctct ttgaaagagt aaagagagca aagaacaata
    74821 tcggttttat tgcaatctct gcaatcgctt cagttcttaa atttcttcat gctaaagaac
    74881 taatcgagac tcaaggcttt tttgcaacag aaacttatct tttaaaatta aagaaagaaa
    74941 gtcaagagaa gaagtcaaaa gccttgtctg cgattgtcaa ttcttcagag tttgtagagg
    75001 ttcttaagct tcttgaaaaa gcgataacca agaggtgtgc aaaggaaatc gctcttcttg
    75061 aaattgtctc ttctttctta aaggaaaacc caaagtcgcg aattcttgtt tttaacaact
    75121 acagggataa tgccaatcat cttgtagaag tgcttaatca aacggcagaa ataaaaagcg
    75181 caagatttgt tggacaggct agcaaaagtc tatctgataa aggattatca caaaagaatc
    75241 aaacaaaaat aatttctgaa tttcgtgaag ggctctataa tgttctagtt tgcacatctg
    75301 ttggggaaga gggccttgat attccttcag ttgaccttgt tgtcttctat gatgccgtag
    75361 cttcagaaat ccgtaacatt cagaggcgtg gaagaacagg aaggtttaat gttggtaaag
    75421 ttattttgct tattaataag gacactatcg atgagaaata ctactttgtc tcccaaaata
    75481 aggaaaagaa aatgaaaagt attctaaaaa attttcataa accagaaaga aagaaaaaag
    75541 aacaaaaaaa gataagtgat tattagatat gcctcaaaaa ctaaagattg tatgtgacac
    75601 aagagaaaga gttagccaaa t
//
LOCUS       DAOOON010000002        49400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_775_length_49400_cov_18.815361, whole genome shotgun sequence.
ACCESSION   DAOOON010000002 DAOOON010000000
VERSION     DAOOON010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 49400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49400
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_49400_cov_18.815361"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..609)
                     /locus_tag="PLK55_00465"
     CDS             complement(268..609)
                     /locus_tag="PLK55_00465"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOZ35445.1"
                     /translation="MRSILRSKIHKAIVTDANLEYIGSITIDKTLMDFVDLIDGEKVL
                     VVSNTTGNRLETYVITGEKDSGIICMNGASAHLIKKGEEIIIMGFEITDSLISPKKIL
                     VDKNNKFIKYF"
     gene            complement(606..1517)
                     /locus_tag="PLK55_00470"
     CDS             complement(606..1517)
                     /locus_tag="PLK55_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ35446.1"
                     /translation="MSETIYDIAIIGGGPAGLTAAIYGARYGLKIMVFSDSFGGTVSY
                     AHKVCNYPGFKEISGMDFATKTIEQAQELGVEINFEKVMAIDKQNNSFVVNSDQRTVK
                     AKKIILAIGRTRKQLGVKREEELTGKGISYCATCDGGFYKGKIVGVVGGSDAAVTSAL
                     LLADLADKVYLIYRKEELRAEKAWADLAKANEKIEILYKTEVLELLGQEKLEGLKTNT
                     DKTIPLNGLFIEIGSVPNTELLSKLNLNLDSHNYIVVDNKQKTNVSGIFAAGDATNAS
                     DLKQVITAGSQGAVAAYHCYLEIKEKI"
     gene            complement(1514..1924)
                     /locus_tag="PLK55_00475"
     CDS             complement(1514..1924)
                     /locus_tag="PLK55_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOZ35447.1"
                     /translation="MKKVKYDCDRKNVKTLRLTDAMSTMAYNIAKEENLTESEFLRKL
                     IEKGLKEYKLGMALKAYKNKEVNIAGGANLAGITYREFLDALEECGIPTIEVSPETIK
                     YGFESMERSLGLKPKKRDYNKLFEDTFINKEKKK"
     assembly_gap    2047..2146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2382..3077)
                     /locus_tag="PLK55_00480"
     CDS             complement(2382..3077)
                     /locus_tag="PLK55_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35448.1"
                     /translation="MAKPRILLKARKAITGKRATPKELGYERDSRGRLTKPGTRAFNE
                     KVQFTKLDRKLFLSEYNRRIKTAKDNADVGIYEQKKIDRKKQYDRKISDLERQREHDL
                     KVAEKNRKNSLKKRQLNDWIKKVDAVLIASRKGTVKEIMNKNFKLIFSQEIDIINKKA
                     VFSKAEAKRAYEYIYQSHVSEFNSLFHKRFNPTALERMSASSIINFLSEISSMAKYNK
                     ASEYNKFRRKFLK"
     gene            complement(3089..3847)
                     /locus_tag="PLK55_00485"
     CDS             complement(3089..3847)
                     /locus_tag="PLK55_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35449.1"
                     /translation="MPKAPAKPILKLRKSSTGTKHTVNEKRTIFDKKWERTRLNPKTP
                     EAKEWSKAQLEKLRKIGKENEKVRKSQQGVLEEYYKKSKSPVYNREPVYKPRGVFVNV
                     PIAEIRLDPKTGTMVFTQDYQGRPKKYRITREELLTNNYYQEVGLFNSLLESRKYSFE
                     ESSASVKKRIETKKQKIIDVARFLKIDYLKNAGVTLDSLITAKFTLPEIIRFGGYSPA
                     ELHSFGITDAQIQTAREYNVKNKSKFDKNYGDFV"
     gene            complement(3969..4874)
                     /locus_tag="PLK55_00490"
     CDS             complement(3969..4874)
                     /locus_tag="PLK55_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016358054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HOZ35450.1"
                     /translation="MAMKCPECGKDKVYKDPRTGELVCKNCHTIISEDMVDMGKEWRN
                     FDQSPGSEQARSGSPMKYVKINKGLETMIERSGRDLRGNKLSSKSRAQMYRLIKWHKR
                     SSISNSMQRNLSIALTELRRMGSYLNIPESLIETAALLYRKTVEKGLIRGRLIEAVVA
                     AVLYSVCRTYNVPRTLNEMAEASGLTKKEIGRTYRFIVKELEIAVPLTDPTYYISRFT
                     SVLGLSGAVEEKGKEIVKKAISSGLISGRGPTGVAAAAVYIAALMVGERRTQKEVANV
                     AGVTEVTIRNRYRELKSKLKLNIDA"
     assembly_gap    5009..5108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5206..5754)
                     /locus_tag="PLK55_00495"
     CDS             complement(5206..5754)
                     /locus_tag="PLK55_00495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35451.1"
                     /translation="MKYVDSVILKNKKFKIYRINQFEVPIFKKLHLEIIKDVPSEFDS
                     PYNYSRYKEDIKLGNDLIFVMYSDKNPVAYGRISFRKADSKNINGLVKHYGINKKELT
                     KVCEIDGVGVISKFRGYGIQDYFLKFRENYMKDKGYKYLFTATHPKNKYSLNNIIKNG
                     YQKQKLYKNYKGVDRLLFKKTI"
     gene            complement(5769..7211)
                     /locus_tag="PLK55_00500"
     CDS             complement(5769..7211)
                     /locus_tag="PLK55_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35452.1"
                     /translation="MIQITTWSTLFEGVYGQKDLAILFNDDYFVSLLRSKEPSSVLVQ
                     LYKVFAVDGDIELFAETLPNQCIVFEKHFDIGDKNTHKFFILNTDTEIIDSSQINEHI
                     EKKMLKLNKITSSIISIIKSYNIKLLSLKMLPPEIRNYFFSDPFIVKALTNSPNNIEF
                     STAISSVDKLLVGRKNGVTINASLENLRSIVVTGAEEEDRVFAMKVIAENYLLSSKKV
                     VVFDSTGVFKTLSYPQQKEELLGAFDMKVSPFGFPINTIDFFKIKLPLHVIPKQAFIN
                     LFKFTGVTQKIIDQAYTDKVKDISELIENIAAIGITDEITDFEKWRLISKLNIIEDVY
                     SGYFGDTDLSIIFESTYKNIGSSKVFLINRNNPLYLFYIYGILKRLSEEVKEEMLVII
                     PQASEVFNNSIIGNELLTILKENPKLNYCIGAEYIDDFIDKEPAIVRIEVVSNNDVVI
                     RYPDKDPLRLLLRPTLTSSNIEIKKEEVIV"
     gene            7306..7851
                     /locus_tag="PLK55_00505"
     CDS             7306..7851
                     /locus_tag="PLK55_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35453.1"
                     /translation="MDFIMIDATLIKDTITELLEANVDKETIYQTLKDIGVEDSEIDK
                     YYKEATTKEKEQKTETKNPESKPESHPKIETASPKILENIKTEEAIHQKPKATTEDLE
                     KATKDVIDSSIMENQEIDEKITKEDNTQILEINNTNLEIKKQISELENNLGDIKAQIN
                     GLTKIMKDILEENRNILNKLK"
     gene            complement(7854..8168)
                     /locus_tag="PLK55_00510"
     CDS             complement(7854..8168)
                     /locus_tag="PLK55_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35454.1"
                     /translation="MFDFLSFFVIGILIIFSFQYNYYFFAPVIVILYSIFFPGLINLL
                     LVFLLAVMLFITTAEIPQFWLIIFGVCILIIVFVAIFKKGGAPKEEGGGYEDLLKMLG
                     NQ"
     gene            complement(8170..8436)
                     /locus_tag="PLK55_00515"
     CDS             complement(8170..8436)
                     /locus_tag="PLK55_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35455.1"
                     /translation="MNSIILVTSLEDSIWIILFIVFSISIYVWAKKQIGNELIAILIT
                     GFLVFLLFYRYPDLIWLVAIGVGIYWVFGSDLKNTIKGWAGVKK"
     gene            complement(8452..9060)
                     /locus_tag="PLK55_00520"
     CDS             complement(8452..9060)
                     /locus_tag="PLK55_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HOZ35456.1"
                     /translation="MSTASKYIKETIQKEFKKEKDDNYNYKKLYTDRLIKYRHTKEAV
                     IRLDNPTNIPRARELGYKAKQGFIVVLAKVRKGSGLIRRPVNGRKPKRMGVNKITRRI
                     SIQRIAELRAASKYPGLEVLNSYLIGEDGQQKYYEIILIDPNHPRIMQDKDINWICWP
                     QNRGRAYKGITSAGKKNRGLDRKGKGTEKNRPSIRAKDRKAK"
     gene            9200..9907
                     /locus_tag="PLK55_00525"
     CDS             9200..9907
                     /locus_tag="PLK55_00525"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOZ35457.1"
                     /translation="MRNMHLFSSKEAKKKVIIALVISLFLVVAAAVFIWPYVSILTKP
                     SVIKELVLGFGVWAPLAFIGLQILQMVFLFIPASPIIIVGGYIFGAWGVFYSVIGIIL
                     GSIIIFFIGRLFGRPFLESVLDKKIINKIDYESDNIAKTLFILFLIPPLPHDAFCYMA
                     GITKIELKKYILAVAVGRLPQIVFLTLIGYQLTKLNLFWSLLLIGVIIVGSIIIFQNK
                     NKIETRLHGYVGKLQKK"
     gene            9908..9980
                     /locus_tag="PLK55_00530"
     tRNA            9908..9980
                     /locus_tag="PLK55_00530"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9941..9943,aa:Val,seq:gac)
     gene            10009..10611
                     /locus_tag="PLK55_00535"
     CDS             10009..10611
                     /locus_tag="PLK55_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOZ35458.1"
                     /translation="MISKLIALLVLFFVIIDPLASFAVFFVNTKNYKKKEKIKIAIMA
                     VAIAAVLSFLVLLFGENLLTIFSTDINSFKIAGGIILLILGINMSLGRSIVEVEKSNA
                     DSVHAIASVIATPLLTGPATITAIIISAADYGKVLTGVAIGIILLLTGISLYLTIIFH
                     HLTEKAKTTIQIISTMLGLITLAWGVNYILTGVQAIFHLI"
     gene            complement(10608..11273)
                     /gene="rnhB"
                     /locus_tag="PLK55_00540"
     CDS             complement(10608..11273)
                     /gene="rnhB"
                     /locus_tag="PLK55_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOZ35459.1"
                     /translation="MTKQLISGVDEAGRGCVLGPLLLAVCVIDKNKEKMFKDAGVKDS
                     KLLSPKQREELFELIKNEAEDYKIVAIPAEELNVLMDRFSLNEIEAQKACFALSKLKV
                     KPEIVVFDSPDTSTDHFSKRIKDNLKAISCDYKFKVISEHKADLNHVSVSCASILAKV
                     TRDKMLKELVGSDISGYSSDPRTIDFLKDYFKKYKSFPEYTRMKWKTIDNIVKDAFQR
                     KLL"
     gene            11358..11621
                     /locus_tag="PLK55_00545"
     CDS             11358..11621
                     /locus_tag="PLK55_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35ae"
                     /protein_id="HOZ35460.1"
                     /translation="MQGTIMNYRMGVNTQKNNHLLIRIDDEKYRTKESVAPLFGHKVV
                     FTTIGKKVITGKVASAHGNKGIIRVIFERGLPGDAVGQKVEIK"
     gene            11625..12392
                     /locus_tag="PLK55_00550"
     CDS             11625..12392
                     /locus_tag="PLK55_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35461.1"
                     /translation="MTKHKDLFFKYTISRLKTELERADQRIIAISQILSSLPKNINEI
                     FEELRIIGDILYPNMEKELTLEEYVRLCSLEEISENTLKKISLDKTKIRKILEIQEMG
                     LSAELEKDEKIIFINLAKNLQALLDTKKSLEALEEKMVKTNYPNFSEIATPAIACKMI
                     EVAGSFERLSRFPASTIQLLGAENSFFRALKTRSKKTPKYGFIYAHPLLINLNKKDKG
                     KFARTISAKIAIAIKADLGGKDLRKELTDKINSKLKK"
     gene            12402..13007
                     /locus_tag="PLK55_00555"
     CDS             12402..13007
                     /locus_tag="PLK55_00555"
                     /inference="COORDINATES: protein motif:HMM:NF003276.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HOZ35462.1"
                     /translation="MAIKNLIKENGDFFTLDLNNKEKRYWDPTRSKVCAALKKGLNFL
                     PLKADSTILYLGAAEGYTCSYISDLAFQGIIFGVDFSAHSMQKFVLLCKERENLYPIL
                     GDANKPEDYKQLVDCKVDLIIQDVAQKNQIEILTKNAKEFLKPEGYVILSLKTTAISQ
                     RNTGVIIDEEIRKFEKEFTIVDKRRLDPFEKKHMIIVGKKK"
     gene            complement(13008..13202)
                     /locus_tag="PLK55_00560"
     CDS             complement(13008..13202)
                     /locus_tag="PLK55_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOZ35463.1"
                     /translation="MECKKEINKKSCNCTYDCEKKGLCCECVSYHRKRGELPACYFDT
                     KAEKTYDRSIDAFLKSKGIK"
     gene            complement(13214..14146)
                     /locus_tag="PLK55_00565"
     CDS             complement(13214..14146)
                     /locus_tag="PLK55_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOZ35464.1"
                     /translation="MLFALIFWILVFLTSLFVLIFSADMFVDKSKELGKNLGLSTFLI
                     GLIIVSVGTTLPELASSIYGTLRGESEIVIPTIIGSVTTNIFLVIGLVGFLYGTKYIY
                     KDTLKTDLWMLAIVVCLFVLFIIDGNFTLLEAFLCVILYLAYSIYQYKLSKPVKLEDP
                     TKSNSYLCVFFILIYSILIYVGSKYVVDSMLNVSRIIGVFPEIIAITVIALGTSLTEL
                     TVGLISVKKGHLDIAVANIIGASIIDILIVSSFSRFFGFVIVPSHIFKIAIPMFGLAL
                     ISFIYVIIDKKLTKKEGLALLLLYIIFNIVLFMI"
     gene            complement(14154..14645)
                     /locus_tag="PLK55_00570"
     CDS             complement(14154..14645)
                     /locus_tag="PLK55_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ35465.1"
                     /translation="MPIVILETTKGNIEIELDTNHAPITTANFLAYVNSGHYDGLVFH
                     RVIKDFMIQGGGFTISGVQKDTNSPIVLESNNGLYNTEGTIAMARTNAPNSATSQFFI
                     NTVDNDFLNYTPGNPGYAVFGKVISGMEVVKAIEGVQTGTKGGHEDWPIEDIIITKAY
                     VKK"
     gene            complement(14750..15259)
                     /locus_tag="PLK55_00575"
     CDS             complement(14750..15259)
                     /locus_tag="PLK55_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35466.1"
                     /translation="MPFKYREKIRLKKPGHLKKIEPKLDLDIKNIHKTLDIKTKPIKK
                     PVDLMDTITEIKTKFMHIIDPFVSGVYKKNSHPRFVYDSAKKTIKLRYQLMSLKDNLS
                     DFVFQLRLYVISHSDDKENIEKIEKEIKEWEKIINKTLNEIIDNVSFVIENYFVPIVN
                     KLEKKKVNA"
     gene            complement(15274..15345)
                     /locus_tag="PLK55_00580"
     tRNA            complement(15274..15345)
                     /locus_tag="PLK55_00580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15311..15313),aa:Thr,seq:cgt)
     gene            complement(15355..16869)
                     /locus_tag="PLK55_00585"
     CDS             complement(15355..16869)
                     /locus_tag="PLK55_00585"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOZ35467.1"
                     /translation="MATRLEKFNEFCATRSLYFPSAEIYANNFAGFYEFGPIGNRIRI
                     NLINFWREEFVRKNNFIEISGSIILPEDVFQASGHLKNFNDPIATCQKCKTVYRLDRL
                     ISENTKKEVPESMSIEEYEKLIADNNIVCSKCKGNLTDIKKFNLMSSINVGTQSGNLG
                     YLRGETCQSIFVDFLRIYKTGRQTLPLTISQHGSVYRNEISPRNGLLRCREFEQLETE
                     MFFDSDKIDETPIPLDTISKYKIRFKLLKDKQEKDYNVSKITEKKITSGNLITYTLYI
                     TQKFLEDLGFSHDNIRFRELPDNDKAFYSKQTFDCEVKTEKEWVELFCVNYRTDYDLL
                     AHGKGSKKDLSISEDGKRIQPHVLEVCSIGLGRLFYVLLENSFEIKEINGEERNILHL
                     NPKVSPYFVAILPLMKKDGLSEKATEIFNALKEEQKRYDIFYDEAGSIGKRYARLDEI
                     GCNYCLTIDHETLEKQTVTIRDRDSQEQKRVSVKSLGKLLEKLYYGKTKFLKIK"
     gene            17049..17130
                     /locus_tag="PLK55_00590"
     tRNA            17049..17130
                     /locus_tag="PLK55_00590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17083..17085,aa:Leu,seq:tag)
     gene            complement(17145..17759)
                     /locus_tag="PLK55_00595"
     CDS             complement(17145..17759)
                     /locus_tag="PLK55_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35468.1"
                     /translation="MPKFAGVRPKPKEKAEKKKKHGKIVKRPAIPEKVKTKLLKDANK
                     LRELKIQVQKMKLGTALLSEKFTEVSKNYDVLQHFEAMANAELDYISEIKAGKKVNEP
                     ILKFEEAVEKFRQDTRTAQSFIERAIGNKLQSLYERVLESEGKNLNKLSDKQREKILT
                     NLVNSFNEYQIIKSKFSQLKFGDTKGIDYRVEKMKKIIESNNRY"
     gene            complement(17849..18475)
                     /locus_tag="PLK55_00600"
     CDS             complement(17849..18475)
                     /locus_tag="PLK55_00600"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOZ35469.1"
                     /translation="MKKLVSQINALKKTAIEKTISKKIKQFKSFSKKSSQDIFQELCF
                     CLMTANFNAKRAVEIQEKCGCLFLTGTEAQLAKLLKANGHRFPNTRAHFIFLAQEHKD
                     DIKEKLFSIKDKYEKREWLVKNIKGLGFKESSHFLRNIGFFDYAIIDFHIVDILADNK
                     IVKKPKNLNKDAYLKIEKKLEGLAKETNLTLGELDFYLWYMETGAIYK"
     gene            complement(18518..18892)
                     /locus_tag="PLK55_00605"
     CDS             complement(18518..18892)
                     /locus_tag="PLK55_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOZ35470.1"
                     /translation="MVKKGEIYRCAICGNVVEVIDAGGGTLVCCGQNMDLHHDTSEEQ
                     LKEKHIPVIEKTRDSIVVRVGDIPHPMTAEHHIKWIEVNIDGSVYVKKLTPGDEPVAE
                     FKGGGYKISARCLCNIHGVWKS"
     gene            complement(18907..19485)
                     /locus_tag="PLK55_00610"
     CDS             complement(18907..19485)
                     /locus_tag="PLK55_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOZ35471.1"
                     /translation="MKTIENLCKAFIGESQARNRYTFYAKVATKESLFQIADIFLETA
                     EQEREHAKQLFVLIRELKKQNNITEDLVVEADVPTAYGNTADNLRAAISGENHEYTSM
                     YPEYADVAEKEGMPEIANKLRAIAIAEKHHEERYKKLLKNLDEKTLLKKEIEVVWICS
                     KCGYMHKGKEPPKECPSCGHPPEYFRVQNENY"
     gene            19577..20404
                     /locus_tag="PLK55_00615"
     CDS             19577..20404
                     /locus_tag="PLK55_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35472.1"
                     /translation="MTEKIIEELSQIKFKATTIYESFKYPLHRTNKTQRIPLLKNIKN
                     NIEELTRLLNGLLGYSSIQFNKEIEAEKIRLFQNVFLEISGKLQQSIFLEEHTTIVEN
                     DLVNIKNEIATPEKYFEIEQSVKNNINNLIIFIDDAKKQIDHKYVSLANQKRSVEELL
                     KTLERKDQTILELNKRIDELNWIDAKEKAKTSRLANLEEELLKTYKANEQDLTILKLH
                     IIHIEKALDNLLRESKSLTQDINHLEAKNILKEQASLELIKELKKELIAAKYNLKNK"
     gene            20411..21937
                     /locus_tag="PLK55_00620"
     CDS             20411..21937
                     /locus_tag="PLK55_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35473.1"
                     /translation="MSEYDPFGGRFPQDSEKPKSNFKLDEKTIGLIKKVIILVVVLGV
                     IGGAIYFFFFNNIIINFSVKDTEGKALNSANIKITPEKQNKSIQYSPGDEIKLNKNKN
                     YKYAIELKGYKTINGQLAPKDIVEPNQAIKLEKDIRLDVLSFICPTDVFIGQTVKCEL
                     QLNNISENEDYNLENLIFRDDKDKPLKNWADLNDEFLRFVDGFDAEVPSTKIITHKTK
                     DTVFILFSVPESIKRGTTQKIMARIKYTDNNAIQEIKISQAPNIGFTSELSSALNLES
                     GNEIIKTYTIDNSKNKTDLSGLELEIDANYEPTTGELDSNFDIAEIIVPDYTTLNVNA
                     SSRSQGQITIKLPNNLRAGKITGILALNSPIFSEPKKITFTINIKEPENKFNISLNKS
                     TETLDYDANTNITTIKQITLNLDNKNKIPVHINSIYIENSTGTTDCNHWIVLPTEYNG
                     YDIQSNYNPAPIILLQGSDLGSLTTITGLKICNINVDYRHPYLDENIIIKNKIQISVN
                     "
     gene            21938..23959
                     /locus_tag="PLK55_00625"
     CDS             21938..23959
                     /locus_tag="PLK55_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35474.1"
                     /translation="MDYIDWIISSVLFITVIAFVLISLFNILPKSNNTNTISDLLYKS
                     TISLIIPVYNVSMISEDSEVYPYVISDCNYGRAENTFEIDTNNNLLFGLIYDKDKFYN
                     YETDINALSGLGVRLLLENFNDYNYQDTFDLNSGTANISSGILELEAGSTIDTTIEFS
                     KYLGSFVTNAEDINVYINFDTTSDRFYCSFNETGVQLFKIGESEAIEENNTTLKTTNW
                     RKLNFGYYRDFEGFSWFFCGIGENINILETSSQTHPEETTIRIEAIDDQTYIDDFRVY
                     KSLINITEHDGTTVKTDSLRTTVTSADMRVEFYELSEMDANEVLGCALSFSTSSGNLN
                     NYNEGPIKIFSNDSNQNKLVFFPIAKEFWIYKYTGEDPITITYIVDEIKPYNFGSATE
                     NGQFVDLNKIPGSYAVPVSFVPENSFTTENFGSFDVNFNKVFSEITPTGGGSETPIIQ
                     YCKVEGCSDITSDSNFIFDTDTNTAEVIFKFPEETVANQPFNIVIIFNYFNLENLENA
                     TGEATVLDGTQLTARVSAEEIIIDTNQFYLYNIFNPIYPNIKQTIIFDVFDNTGLHAQ
                     DCNFFINWGSQNIVVTCDENYLLKVRYRFTDLNNTILDYPNLSITKTNERIVTQEHFE
                     NLSCDSYFVNIKNKTLDVNCGIGVNPNYKMIIKYLHNTGLLENADIYIK"
     gene            complement(23976..24140)
                     /locus_tag="PLK55_00630"
     CDS             complement(23976..24140)
                     /locus_tag="PLK55_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HOZ35475.1"
                     /translation="MKMNYSKNNNQGPSSAIGIMKFKESVAGPQLSPEAVIGLSLGIA
                     IVILILRAII"
     gene            24234..25163
                     /locus_tag="PLK55_00635"
     CDS             24234..25163
                     /locus_tag="PLK55_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35476.1"
                     /translation="MVEQKQSEYKWLVRIIFLLIIIVAALFVFIWTGTIRCRSIPGGC
                     NVYWSVMTIVTGKQQPSVLILYDPTDQKGLGNPELLKDLLTDRKGVAIHPSLADIRYL
                     NSEQLKNVSLVVVEKARKISTLQLMTMRNYIAQGGRIVWIGDAGIEHNEDKGDLLYVG
                     PENEKKNGWVRLTDANMIIFFNRTLGVNYVANFCDIRDCDVGDKFTGKLMPSSDTHPL
                     VYGLKPNLPISDNYSIVTLIEPNPVPLKIDFGANLRDTNNNGYGDIFDAIVTSNSNRV
                     AYYAIPPEYLAEDKDGENKYFSIIYNMFEGMVN"
     gene            25165..25518
                     /locus_tag="PLK55_00640"
     CDS             25165..25518
                     /locus_tag="PLK55_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35477.1"
                     /translation="MTKSFEVAISIILLLFFVFFLFETINQKYSQNPIPEEVKTIILL
                     EARDSGFRELISNSEVDNVYSLLYPQMDYRFNVSICDWLDDNCENRELENFAKKREFV
                     YFFSDTNKTLHIELD"
     gene            25531..26010
                     /locus_tag="PLK55_00645"
     CDS             25531..26010
                     /locus_tag="PLK55_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35478.1"
                     /translation="MKKGFTYIVTTMLLIILISGVVIVSNYNAPGNKINPVAILAKNY
                     ENEFLNILNSGSSVEDINQFNYRFKKFINSNNYDSKLCNLITTENGIIASNFMDQNCD
                     LMINGVSNQTITENNTIITDIFINDTNIYLCNCYYKAGENIYYIDIYNENSKTTFKN"
     gene            26021..26371
                     /locus_tag="PLK55_00650"
     CDS             26021..26371
                     /locus_tag="PLK55_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35479.1"
                     /translation="MKNVTELLNQIEERTNIIVAKLEILRFAEEDVQTIKKSLEVIKK
                     KLESGEISKFSYATMLEINKKRAMENTNSKKKTWDEVAESINEISDLLNDLKDTFNAK
                     TEFEGIDEIKTSKS"
     gene            26405..28387
                     /locus_tag="PLK55_00655"
     CDS             26405..28387
                     /locus_tag="PLK55_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOZ35480.1"
                     /translation="MKIPNQKDASKLYDLILNLKNIENQRTAKEQEILTKFYNQELDS
                     KKLVSELKKEKLFSNSKKLDLNFINTFIEYSKQLEFLSENIDVTTIKEYKLTKRPAPF
                     ILGLKKIFSSKTTKISEKKKSAKPKLVEVNPKEKKQQQNIFQSLFKNLKTDKETIKIK
                     ENANPLPSFEEPKELLPENYQILIEGYAYAFVEEKEGSRRYVVAEPELTSAEEKILKE
                     TKQELINKISLIEIKDESELIKRIEKIFNKTKITPQQKSKIMYYVGRQIKGLDLIEPL
                     MHDPLIEDIECDGTGIPIFIVHRKYGHLETNIAYKDEKELQQFVIKMAHLSKSYVSYA
                     SPLLDSILPDGSRVNATLTTNVSTRGPTFTIRKFPHTPLTAIDLITSGTLNPMIVAYI
                     WTIMEFQKTALIIGPTAGGKTTLLNVITSFIPPGRRVVSIEDTREINLLLDNWVPQIT
                     RPGFGPPDGTGKKYGEVTMLDLIKESFRQRPDYLIVGEVRGEEMSIMFQGMASGHCCL
                     STVHARSLPDLVNRLITPPISLEPALLTSLDLVIVTGFEGGSDIKRTIKELDEIKGFN
                     SKENKIEYTAIYNVYERSQEKDGDLFTHELPITYKSEILQSIAREYNININHLLEMIN
                     KRIKFVEELTKNPPKDYVEFKIAINKYKLQEKVITN"
     gene            complement(28390..29697)
                     /locus_tag="PLK55_00660"
     CDS             complement(28390..29697)
                     /locus_tag="PLK55_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35481.1"
                     /translation="MKKIIAVMFLLFIIPMAQAISLYSAQDIAKEYMLSNNEYLDPGS
                     VYYIDCDNNEYYTLNIVDAESNIVLFLPIESNNGSVIYEINTNTQNIIKTALLNRNIK
                     TKSGNNYLSQQLVDKIDNLNITLQSKSAKLDGIIRSNYSSSITTKCQNTKDKLELLIN
                     QLQDMKTKLSKLQKEQSTFLDTTDCTKADSLILLYKNTFKGYNDIVSSSINYRDSTNE
                     IVEVIVADQKLDETTKKIILSYIEAPNNLNGDISTITDSLASTNQFYSLVIADFEKAG
                     PNSPLEILQQTLETRQNYYVAKSLLYNFNTDLKGTLDNSINQILNSENINLWKDTKTV
                     SELSQNYAQIKELYNKGRYEESISKIKLAKNQVKKIKDAGINEIETQDLTKYFIYIIV
                     ATIVVLVSITLVKKIKIKNKPKQKTKSKDIDPEYLFNRRDPFK"
     gene            complement(29694..30254)
                     /locus_tag="PLK55_00665"
     CDS             complement(29694..30254)
                     /locus_tag="PLK55_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35482.1"
                     /translation="MFNQTTDYLSKLTIFLIIGLFISSAIYILPEQNKKVEEFEAGLV
                     NLFIADNIDPWISTSYIINQQCTLSNLKYIMESREYLELRIKTDETSSDFTDFMENLK
                     TSAAANKTTAEIKELMPDLDAPVIKVKIIDTIKAIPFMGFIESNFAFFFALAFSSFVY
                     TGLSIVFLIFAAIVYLFNKIFKEEEE"
     gene            complement(30668..31594)
                     /gene="map"
                     /locus_tag="PLK55_00670"
     CDS             complement(30668..31594)
                     /gene="map"
                     /locus_tag="PLK55_00670"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HOZ35483.1"
                     /translation="MNKTEIDILKKSGIINNGAQKFAKKIVKPGISFLEIGAEIENYI
                     KEKGAKPAWPVNLSINQEAAHNSYNPEEQVILKETDVLKVDVGVSIDGYITDSSQTFV
                     FSKIHEKLKESSDKALLNSKDYILKNPKIVTVSKVGEIIEDTIKGYGYKPISNLTGHT
                     ITRYTTHNHPSIPNVKNNINIKLSEIDSWFAIEPFSSTGNGYVTEGSNVYIFIFEKDV
                     SIRNSNARKILEEIKSFEGMAFSEFWVGKNLSSFDRKVAFRELLKAQAISAYPVLIDR
                     NGSYVSQAETTFVYDKENQEMIDLININEIIE"
     gene            complement(31591..32085)
                     /locus_tag="PLK55_00675"
     CDS             complement(31591..32085)
                     /locus_tag="PLK55_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35484.1"
                     /translation="MKKIFIFAFLILMSGTIFADFYIGEIIAPKAVIEDTNFDINVIV
                     VNTSSTTQDVNLRIEVFTPQAVQIFDRNYFEGNAISIGANDSNNIFVNIIIPDSNAST
                     AQHLIRATILEDDDNPINNIGQIWFMIKQAQKNVPIPDMPIYFGFIVAFIAVVFLTNK
                     KEKK"
     gene            complement(32168..32638)
                     /locus_tag="PLK55_00680"
     CDS             complement(32168..32638)
                     /locus_tag="PLK55_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOZ35485.1"
                     /translation="MPTIKVLVDAGKASAGPPLGPALGPLGVNTMEVVKEINRVTKEL
                     EGMQVPVKVHINSDKSYSVEVGAPLTSALIKKELNIKKASGKAGTEIAGNITFDKILK
                     ITKAKYGQLSARSFKAGIREVFGTCRSLGVNIDGKTPAEALKDLNAGNYDSKIA"
     gene            complement(32655..33092)
                     /locus_tag="PLK55_00685"
     CDS             complement(32655..33092)
                     /locus_tag="PLK55_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HOZ35486.1"
                     /translation="MIFTVRTTVGQERVVIDILHHRIKEEGLPVYSLVEMADLKGYIL
                     IEAQEEKTVELLIHNVPHIKGGQMVTGNINIKEIAGILESRPLMSSIKPGQTVEIILG
                     PFKGSRAKVTRVNVAKEEVVIELFDATVKVPITMKAENIKLLN"
     gene            complement(33089..33268)
                     /locus_tag="PLK55_00690"
     CDS             complement(33089..33268)
                     /locus_tag="PLK55_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HOZ35487.1"
                     /translation="MVNKIKEFFESSKRILLIAKKPGKKEYWAMAKIVGIGMIVIGVI
                     GFIVRIIMNIIGGKV"
     gene            complement(33281..34387)
                     /gene="ftsZ"
                     /locus_tag="PLK55_00695"
     CDS             complement(33281..34387)
                     /gene="ftsZ"
                     /locus_tag="PLK55_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOZ35488.1"
                     /translation="MQSSRVVAQDENSEKVQRSDDEELEEFLKASLPNIYVIGVGGSG
                     GNSLDRMNQTGIEGAIMIAMNTDAQALLKIRADKKMLIGKKTTRGLGAGSNPEVGRAA
                     AEESKDEIKNVLKDADMVFVTCGLGGGTGTGAIPEVARIAKEKGALIIGVVTLPFAVE
                     GKQRIENALGGLERLKKEVDTLIIIPNDKILEIAPDLPLQIAFQEVDKILTQSVKGII
                     ELVTKAGIINLDFADLRTILSNGGLAMIGVGESSINKNNNDSRAMEAVEDALTSPLLD
                     IEIGNARKALVNVLGGQDMTLKEAELIVETVASKISQDAHIIWGAMVDEDMPRNSIKV
                     MAVIAGADIPYLSDIVGTNIGKKKEKLELNIESV"
     gene            34547..35455
                     /locus_tag="PLK55_00700"
     CDS             34547..35455
                     /locus_tag="PLK55_00700"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOZ35489.1"
                     /translation="MIDKDILRIGKDIKEIKIQGASKIELAAVNGIKELINKSKFEGN
                     DFLGVVVENINHLISQRPNEPKLRNSLSYIWQMSNKYFSIKDRNLLVDLIENYERNIT
                     QNNELISEYASQLIVNGSTILTHCHSNLVENAFKKAYDRGTDFEVICTETRPRFQGRI
                     TAKALSEYGIKTTQIVDSAVSGYLKKIDFFMTGADVIFQDGSIMNKVGTHTISIVAKN
                     FKTPHIVLTSTHCVETDVFVSLNESIEERDIDEIWKVEDGRPKDLKLRNLSFDIVPNE
                     LIDKFITEEGVFSPETLALWVRKNLK"
     gene            complement(35474..36304)
                     /locus_tag="PLK55_00705"
     CDS             complement(35474..36304)
                     /locus_tag="PLK55_00705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOZ35490.1"
                     /translation="MNYYNSLDFNLHQGDCIKILKEMPDNSVDLIFADPPYFLSNGGV
                     SCKAGKMVSVNKADWDKSNGVVKDFEFTFDWLSECRRVLKDTGSIWISGTMHNIYQVG
                     YALQILDYQILNEISWFKPNAPPNLSCRYFAHSHETLIWARKEKGSKHKFNYELMKKW
                     EDKISESGKQMRSVWWIPLTPQLEKRSGKHPTQKPLELLKRIVLSTTDKGDLVLDPFN
                     GSGTTGIVSKLYNRRYIGIDLDKKYLDLTIKRVKALDIKVNDYSCINNNLIIENQISV
                     "
     gene            complement(36361..36882)
                     /locus_tag="PLK55_00710"
     CDS             complement(36361..36882)
                     /locus_tag="PLK55_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002819952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35491.1"
                     /translation="MIKSGVGGGKTITGLRFEERISLKQLFTQIKGYDIKNDDIFFNG
                     KKIATFYQKHKIYKNFLEPSGINYLNYISKKLLPDDAIFVLKERTLFIIEIKFQEVAG
                     SVDEKLQTCDFKNKQYNKLFKPLNIKVKYTYLLNDWFKKLEYKDVLDYIKSVNCYYFF
                     NEIPLEFLGLPKP"
     gene            complement(36894..37751)
                     /locus_tag="PLK55_00715"
     CDS             complement(36894..37751)
                     /locus_tag="PLK55_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOZ35492.1"
                     /translation="MAKPFVKWAGGKNQLLKEIISTMPKEIKGYYEPFIGGGALLFYI
                     LENFPPKRVLISDINEELINSFLIIKTEPDELIKMLEKYKSKHSEEFYYTIRGLETPT
                     TKRLGIVLSDPKYNKLDNIERAARFIYLNKTCFNGLYRVNKDNQFNVPMGKYKNPEIF
                     SKKNILEASRLLQNVQMKVMPFEDVIDFAKAKEFIYFDPPYDQINGNSFTSYTSNDFV
                     RSDQLRLKNIFDKLDKKGCFVLESNSPTPFIKEQYKKYKIKEVLAKRMINCDGTKRNE
                     IKEVLIKNY"
     gene            complement(37929..38060)
                     /locus_tag="PLK55_00720"
     CDS             complement(37929..38060)
                     /locus_tag="PLK55_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35493.1"
                     /translation="MVSKDKELLLVAKKLKKEQLEMKKKNLKYLSEKEAMKKYKHLI"
     gene            complement(38081..40540)
                     /locus_tag="PLK55_00725"
     CDS             complement(38081..40540)
                     /locus_tag="PLK55_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HOZ35494.1"
                     /translation="MFNIFKKAPKQDNNQNLKENTGIFIDAYYKESQLFVTIKKDQIK
                     QTYPVDYQPYFYLSLNQKLNSEEIENLKSKENVLGIILKEENVYKLIFENIEDLVKAR
                     TAFFETDFFPNKKYKLYEYDIPFVYRFLLDKNLSYLKEVEYTLNTENKITEIIPKENT
                     DLSKLNICSFDIEVLVPESLSFPKPGNDKIISIAYTDNNKQKAIFFLIDKTDNLEKTK
                     EELQKHYQFLDLHLFTDEIKMIEEFTNFIASKNIDIVYTYNGIFDFEYLEKTYKAKTG
                     KSLSFSNSRLIFSKSQTRKVNLQGTIHIDVYALMRLLRYLQVFNYPKLDLNSLYSKLT
                     GNPKLTLPPKEMRELYLSKDYYKIIDYNLDDTIATIELANNYMPIAFEISDFINFPVN
                     DIVFSSAGAMVERLFFKYYFKKNKIIPNKPRAEEISERQKYTFSGAFVKSPIPGLHKN
                     IAVLDFRSYHISIMISYNISPETVDCSCCDKSSLENITGHYICKNKKGFVPEVLEELL
                     TLRAGYKESAKLLEKDTQEYRNMFAKQYALKIFLASTYGYMGFSGARWYSRSSLEIMY
                     YLVRSKIQDMIRLLESKGYLVIYGDTDSCFIEFRSIDKLKEDLREINNNLPKAMNLEL
                     ENIYMSGLFVHARDKERAAKKKYALLDYNGNLKIKGFEYVRHDWCPLVKETQKEILKI
                     VLKEQDNVKALKYLQFIIEDLKNKEIPNNKLIIQTFVHKSLESYKTLNPAMSAVKNAK
                     KAGKKFKVGDLVEFIITEKGKSISDKSQIAEFVKDKDYDVDYYINNQLVPAILPILEE
                     LGISKDELVTNTKQKGLGEFF"
     gene            complement(40575..41222)
                     /locus_tag="PLK55_00730"
     CDS             complement(40575..41222)
                     /locus_tag="PLK55_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35495.1"
                     /translation="MPATRKTETNLPEAYKPKRGPKAIIDYFFFKIGIKESIFIVLFA
                     IIINILIYLLFKGQDLGIYILNQLLTGVILTWLVLGLAIYLIMYFIRGQKNLPKKPYE
                     KVLSAIAAFRATSIIYTILCALLVFTLFPSIISSLQMLAVNPSLINSATLFPELTLAN
                     MIGAILVFLLTIFMIVYWLIMLYEFTEIGFEVKKPVYKIALTLVLMALIFLISLI"
     gene            complement(41325..42056)
                     /locus_tag="PLK55_00735"
     CDS             complement(41325..42056)
                     /locus_tag="PLK55_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ35496.1"
                     /translation="MSSKLKIKLKGIAPIITVILVVVVAVILGTILLTWSRETSSSSL
                     DNTKTVTNSQANPDNYVYPESVDAGEVSLNYSPPAEIEDQEIMIVGYKVIADNMETIE
                     VTLEDPVPLEEGSNVINLEGFADLEITASTITVILITSDNQFITLENIPNTNVPVRKV
                     ATPTADPVPGTYSYEMISLSTSTDGATIYYTTDGSTPTIESTPYSSEIEFYGADMTLQ
                     AMAVKEGCENSEIFSGEYTISVMQR"
     gene            42151..43233
                     /locus_tag="PLK55_00740"
     CDS             42151..43233
                     /locus_tag="PLK55_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35497.1"
                     /translation="MARKKQVFSKPFNLVYENKYPDLLVGPGFYEEQNIAGDSDPKTW
                     IKKKPEEIERERAKQIFTFKSFRKTDANRFDKNLTEIQLLSQSVDTTEIEVGLETKAK
                     KINENYSGINNFSYGLEDLKIIDNIKIPQSIDKVVSDGDLKAKEGILRLNEKLEDVYK
                     IEQILSMGLLGVTKNRVLVPTRWAITSTDDIISKDLIENTLLDYSTIDKYEVFSFMFY
                     QNIFYVLLVPRLWGFEQIEIQGENVCEDYEINGPRKKYASSVTGGYYASRIEVARYLS
                     KRKRQASVIVFRDISPEYRSKGVWVIRETVKEALTKEPYVFETLEQATGFINQHIRNG
                     ISYWLNHSELYKEIKTQKKITDFFKL"
     gene            complement(43228..44256)
                     /locus_tag="PLK55_00745"
     CDS             complement(43228..44256)
                     /locus_tag="PLK55_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35498.1"
                     /translation="MNYIILILSCLVSFLASFFAITQFKKFFVTRGIIAVDQQKKEKP
                     TLPQSGGIPVFFGFLIGIMFFVLLSAFYFQTNITLIIATTLSILIATLIGFFDDINIS
                     KEPVKNIYGDLEIRVGLPQWLKPLLTVLAAIPLIAIMAGDSTMAIPLIGTINFGVLYA
                     LLIVPILFVVITNSTNMLAGANGLEGGLMAIVMISLGIFSIINGRTEAAVISFMALFA
                     LAPYLLNNWYPAKMLPGDSLTYLFGATFASIAIIGNIESFALFIFIPWGIEIILKLRG
                     KLKVRSLGNLQKDGTLKMPYKRIYSWTHIMMFLPTLFKRRFTEKQVTTSILLVEVIFC
                     ILGFILFL"
     gene            complement(44266..46233)
                     /locus_tag="PLK55_00750"
     CDS             complement(44266..46233)
                     /locus_tag="PLK55_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HOZ35499.1"
                     /translation="MKKIKHFFSKHWLIILIAVLIFVLGLAIRAHILRYDYMFEYDPY
                     WHLRATGYVVQGDLPTNDPLGFYQQGGTNYGIWPNFLWFFTAAVYTITTFGAAYDKWL
                     LMEYARWLPAIFGALISVAMFFLGTKMYNTRTGVAMGVMAATVPAFVYRTMGGFFEED
                     SLGFLWLVLGFIFLVMAIQNRHKLKNWLISSIVGAFFFFLMAITWDMFLLIPLVLLAY
                     FGTMLVYMLIKKINKKEILAFSKVFLTCFLLFTIITTAYSGTGWIKRTSEYVTNYLPI
                     STDNIDRINKENVAAEDVIGATVGEENTGKQFFLEKYSLLVVIPFLALLIIPLYLIFG
                     KYKNKEKENEDYKHDNIDYFVLIIFFWILITGFMAWTKLKFTYTLGLPIAAGFGFLFF
                     LATEKLQHRALAYKRTLTIISAIFLIGAIAAGSFFVMSKVPPIDEAKDWQNAMFWVKD
                     NSSADAKIFNWWDSGHWIAYFTERKVSTDNTNASAEATSDFGMFLISEDLNFSKGMLK
                     KYDADYFMVESSYLTRMSSFAIYGYWTTNFSDPRVTKYYGLGLGCSKVVTPISGAVSY
                     NCQGNVLPEAEMAKIKTEYHTAPDQIMQGMPVYLYKEKDNSRIYLINAAINKTIFARV
                     WFNDPTVEPFIELSYSSGDVRVYSVNKDKLN"
     gene            complement(46370..46487)
                     /gene="rrf"
                     /locus_tag="PLK55_00755"
     rRNA            complement(46370..46487)
                     /gene="rrf"
                     /locus_tag="PLK55_00755"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(46527..47102)
                     /locus_tag="PLK55_00760"
     CDS             complement(46527..47102)
                     /locus_tag="PLK55_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HOZ35500.1"
                     /translation="MEYKIVNLVASSSLGAGLDLYNLATAIPEVEYEPEQFPGAILKM
                     DEPRVSLLLFKNGNIIISGASCEKDIKTAIKKATKIIGEVQKGVTLKERVSYKIVNLV
                     ATSNLNKQIDLFNAAVTLDNIEYEPEQFPGAIVRLFNPKLTMLLFKNGKLICAGAKSE
                     EDIVKGLNKLKKVLAGQKDVNTEEKTSKKKK"
     gene            47345..47659
                     /locus_tag="PLK55_00765"
     CDS             47345..47659
                     /locus_tag="PLK55_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HOZ35501.1"
                     /translation="MANKKAKGKRSLTRRRYKRNVREKTTVNQLIKPIKVEDVVQVNA
                     NSAVHSGIPHRRVDGITGKVIGFQGNCPIVQLKDGNKTKKIITHRVHLKIIKQIAKGE
                     KK"
     gene            47656..47988
                     /locus_tag="PLK55_00770"
     CDS             47656..47988
                     /locus_tag="PLK55_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35502.1"
                     /translation="MINNPNYVDDLSLAEVKKILTEKGKDKELNYEQKLALEHAKQFA
                     KLTPLKAEKLKKELMEMELPNEVATKIVDILPNAIELDLIAEKNKTITDDNKEKILTL
                     VNKYKKDE"
     gene            47996..48526
                     /locus_tag="PLK55_00775"
     CDS             47996..48526
                     /locus_tag="PLK55_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HOZ35503.1"
                     /translation="MIEIKKEAIVLDRATKEIQFKQTPIIQCIGVSNFSLLELYSDET
                     PKLQETISTEDKPIKRITYDKLTTIAKKELEKAIETIVNNNQDRFVTFFNNARPINAR
                     RHQLDLLPMIGKKHRMALMEYLEKNGPIKTLEDLKKIELFPDPIKVIVNRVITEIENN
                     EDEKFFLFTIPIIKKF"
     gene            48538..49344
                     /gene="rsmA"
                     /locus_tag="PLK55_00780"
     CDS             48538..49344
                     /gene="rsmA"
                     /locus_tag="PLK55_00780"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOZ35504.1"
                     /translation="MIKDLYSQMLKYNFKPSHSLDQNFIVDDKVIEKVVELLDIKKTD
                     VILEVGPGTGFLTKHLLEKAKQVIAIEKDKKMFEILKQEFKEKLESKKLLLVNEDILK
                     LNVFDYKFTKIATFLPYSISLQFTEKILNCNCLIVEIVQREFAEKLSSIPGFINYNAI
                     SVLTQSYYEVELKKNVSKKCFFPVPAVDSSIVVLKPRKKEFDKRYNDFVKGLFRYPNK
                     DVSNALNLFSRDVKDRSISEKNKNLENKIRDKKIRQIEITELEKIYSIIY"
BASE COUNT        16826 a         7760 c         6929 g        17685 t
ORIGIN      
        1 attgttgaat ttaatagtgg ctctttttca ctaatatctt gtaagtcttt aatgtcttga
       61 gctaaagaag ataaaatctc ttccattggc ttatatagct ctacaagctt attagtttta
      121 tataaatttt tatttaaaga atcaaataat ttagttaatt ttggatcatc tggcataacc
      181 aatctctcct tttagaaata tatatagatt agaaaaggaa ctatatttaa aggtattttt
      241 taagaaaaaa aagaaaagtt taaatattca aaaatatttg ataaatttgt tatttttatc
      301 aactaatatt ttttttggag aaattagcga gtctgttatt tcaaatccca taattattat
      361 ttcttctcct ttttttatca aatgcgcaga agcgccattc atacaaatta taccagaatc
      421 tttttctcca gtaataacat aagtttctaa tctatttcca gtagtattac taacaactaa
      481 tactttctcc ccatcaatta aatccacaaa atccattaat gttttgtcta ttgttattga
      541 tccgatatat tctaaatttg catcagttac tattgcttta tgtatttttg accttagaat
      601 cgacctcata ttttttcttt tatctccaaa taacaatgat atgcagcaac tgcgccttgg
      661 cttcctgcag ttattacttg ctttaggtcg cttgcgtttg ttgcgtctcc tgcagcaaat
      721 attcctgata catttgtctt ttgcttattg tcaacaacta tatagttgtg agaatcaaga
      781 tttagattta gtttagacag aagctcagtg tttggaacag aaccaatctc tatgaatagg
      841 ccgtttaagg gaatggtttt atctgtgttt gtctttaggc cttctagctt ttcttgccct
      901 aaaagctcaa gcacctctgt tttatataga atttctatct tttcgtttgc tttagcaagg
      961 tctgcccatg ctttttctgc tcgcagttct tcttttctgt aaatcaagta aactttgtct
     1021 gctaggtctg ctaaaagtag agcagaagta actgctgcat cacttcctcc aacaactcct
     1081 acaatctttc ctttgtaaaa accaccatca caggttgcac aatagcttat gccttttcct
     1141 gtcaattctt cttctctttt tacgcctagt tgttttctag ttctacctat tgctaagatt
     1201 attttttttg ctttaactgt tctttggtca gaattaacta caaaagaatt attttgttta
     1261 tcaatagcca tgaccttttc aaaattaatc tctactccaa gctcttgggc ttgctcaata
     1321 gtttttgttg caaaatccat accagagatt tctttaaaac ctggataatt acaaacctta
     1381 tgagcataac taactgtccc gccgaaagaa tcagaaaaca ccattatctt tagaccatat
     1441 cttgcaccat agattgcggc agtaagacct gctgggcctc caccaataat tgcaatatca
     1501 taaattgttt cgctcatttt ttcttctcct tattaataaa tgtatcttca aataacttat
     1561 tataatctct tttttttggt tttaggccaa gagacctttc cattgattca aatccgtatt
     1621 ttattgtttc gggactaact tcaattgttg gaatgccgca ttcttctaag gcatcaagaa
     1681 attctcgata ggtaatacct gcaagatttg cgccgcctgc aatgtttact tctttgtttt
     1741 tgtaagcttt tagagccatt cctagtttat attccttaag ccctttctct atcaactttc
     1801 ttaaaaactc agattctgta agattttctt cctttgcaat attgtaagcc atagtagaca
     1861 ttgcatcagt taatcttaaa gtttttacat tctttctatc acagtcatat tttacttttt
     1921 tcataaacat cacattaatt cttttcttgc tttttctata tattttaatt tcattgattt
     1981 taaactttct aaaatcatta atttttcaag agtttgctct tttgtaaatt ttttttgttc
     2041 atattgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntata attttattct
     2161 aacaaaagca tgtagttctt cttcgacttt tcttgtaaca acaatgttcc atagttgtgt
     2221 aaatgattcc actaaattta atttcactaa atgaatccaa aaagaagtat ccacaaccgc
     2281 ttttggttta gcttttctag ctgccataaa tagatctgaa acaaacagtt tttaaacgta
     2341 atactttgta atacgtttaa gaaaaagaat ataaaaggaa attatttaag aaactttctg
     2401 cgaaatttat tgtattcgct tgctttgttg tattttgcca ttgacgaaat ttcagataaa
     2461 aaattaataa tactacttgc actcattctt tctagtgctg ttggattaaa acgcttatga
     2521 aataacgaat taaattcaga aacatgcgat tgataaatat attcataagc ccttttagct
     2581 tcagcttttg aaaaaactgc ttttttattt attatgtcga tttcctgaga aaatatcaat
     2641 ttaaaatttt tattcataat ttctttaaca gtcccttttc ttgaggcaat caaaacggca
     2701 tctacttttt ttatccaatc atttagttgc ctttttttaa gagaattttt tctgtttttt
     2761 tctgcaactt ttaaatcatg ttctctttgt ctttctaaat ctgatatctt tctatcatat
     2821 tgcttttttc tatctatttt cttttgttca taaattccta catctgcatt gtcttttgca
     2881 gttttaattc ttctattata ttctgataag aacaattttc tatcaagttt agtgaattgt
     2941 actttctcat taaatgcacg tgtgcctggt tttgttagcc tccctcttga atctcgctca
     3001 tatcctagtt ctttaggtgt tgctctttta ccagttattg cttttcgtgc ttttaataat
     3061 attcttggtt ttgccatatt ttctcaactc acacaaagtc tccataattc ttatcaaatt
     3121 tacttttatt tttaacatta tattctcgtg ctgtttgaat ttgtgcatct gtaattccaa
     3181 aagaatgcaa ctctgctgga gaatatccac caaatcttat aatctcaggc aaagtaaatt
     3241 ttgctgttat taaactgtct aaagttactc ccgcattctt taaataatcg atttttaaga
     3301 atcttgcaac atctattatt ttttgttttt ttgtttctat tcttttctta acactagcag
     3361 aagattcttc aaaagaatat ttcctagact ctagaagact attgaataat cctacttcct
     3421 gataataatt atttgttaat aattcttctc ttgttattct atatttcttt ggtcttcctt
     3481 gatagtcttg tgtaaaaacc atagtgccag tcttaggatc taatcttatt tctgctattg
     3541 gaacatttac aaaaacgcct cttggcttat agactggttc tctattatac actggacttt
     3601 ttgatttttt ataatattcc tctaatactc cttgttgtga ctttcttact ttttcgttct
     3661 cttttccaat ctttcttaat ttttctaatt gtgctttact ccattctttt gcttccgggg
     3721 ttttaggatt taatcttgtc ctttcccatt tcttatcaaa aattgttcgt ttttcattaa
     3781 ccgtgtgttt tgtgccggtt gatgatttcc ttaattttaa aattggtttt gctggtgctt
     3841 ttggcatata atctccctcc attaattatt aattataaat aattaaatga ttaatagaaa
     3901 tatatttaaa tgtttagaat aagaaaaaaa tagaaaccca aaagaaaaag aagatgctta
     3961 aattgacttt aagcatcgat gtttaacttt agcttagatt taagctctct gtatctgttt
     4021 cttatagtta cttctgtaac gccagcaacg tttgccactt ctttttgtgt ccttctctcg
     4081 ccaaccatta gagctgcgat gtaaactgct gcagctgcaa cgcctgttgg acctcttcct
     4141 gagattaagc cagaactgat tgcttttttt acaatctctt tacctttctc ttcaacagct
     4201 cctgaaagac ctaaaactga agtaaatctt gagatgtaat aagttggatc agttaatgga
     4261 actgcaattt ctaattcttt aacaataaac ctgtaggttc ttccaatttc tttctttgtt
     4321 agacctgaag cttctgccat ttcatttaat gttcttggaa cattatatgt tctacataca
     4381 gaataaagaa ctgcagcaac tactgcttca attagtcttc ctcttataag tcccttttca
     4441 actgtctttc tgtaaagtaa agcagcagtt tcaattaatg attctgggat attaaggtat
     4501 gaacccattc ttcgtagctc tgttaaagca atagaaagat ttctttgcat ggagtttgag
     4561 attgaggatc tcttatgcca tttaattaat ctatacattt gtgctcttga ttttgaggat
     4621 agcttatttc ctctaagatc tctaccagat ctttcaatca ttgtttcaag acctttattt
     4681 atcttaacat acttcattgg tgaaccagat cttgcttgtt cagaacctgg agactgatca
     4741 aagtttctcc attcctttcc catatcaacc atgtcttcac ttataattgt gtgacaattc
     4801 ttacaaacaa gttctcctgt tcttggatct ttatagacct tatctttccc acactctgga
     4861 catttcattg ccatttttat cactcctctt gtattcttaa cttttttctt tgcataattt
     4921 taaaaataga gcttgcacga ataaataatc ttaaaaaatc aaactaaagc aaacctatat
     4981 aaatattatt cttttttcta gtatatatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnaa aaatcaaact aaagcaaacc tatataaata ttattctttt ttctagtata
     5161 tatagctttc ggttttagac ggttttgaaa caaaaatcac aaaaatcaga tggtcttctt
     5221 aaacaacaac ctatctacac ctttgtagtt tttatagagt ttttgtttct ggtagccgtt
     5281 ttttattata ttgtttaaag aatatttatt ttttggatgt gttgcagtaa ataaatattt
     5341 ataaccttta tctttcatat aattttctct aaatttaaga aaataatctt gaataccata
     5401 tcctctaaac tttgaaataa cacctacacc atcaatttcg caaacttttg ttaattcttt
     5461 tttatttatg ccataatgct ttactaatcc gtttatattt ttagaatctg cttttctaaa
     5521 cgaaattctg ccatatgcaa cgggattttt atcagaatac attacaaaaa tcaaatcgtt
     5581 tcctaatttt atgtcttctt tatatcttga ataattatat ggcgaatcaa attcgctagg
     5641 aacatctttt ataatttcta aatgcagttt tttaaaaatc ggaacttcaa attgatttat
     5701 tctatatatc ttgaattttt tgtttttcag aattacagaa tctacgtatt tcatattaat
     5761 cttataaact atacaataac ttcttccttt tttatctcaa tgttgctact agttagtgtg
     5821 ggccttaaaa gaagccttag tgggtctttg tcaggatatc ttataacaac atcattatta
     5881 gaaactacct ctattctcac aattgcgggt tctttgtcta taaaatcatc aatatattct
     5941 gcaccaatac aatagtttaa tttaggattt tccttaagta ttgttaataa ctcattgcca
     6001 ataatcgaat tattaaaaac ctcacttgct tgaggaataa ttactaacat ttcttctttt
     6061 acttcttctg ataatctttt tagtatacca taaatataaa ataaatataa cggattattt
     6121 ctatttatca agaaaacttt actagatcca atatttttat aagtgctttc aaatattata
     6181 gaaagatcag tatctccaaa atatccagaa tatacgtctt caataatatt taatttagag
     6241 attaacctcc atttctcaaa gtcagtaatc tcatcagtta ttcctattgc tgcaatattt
     6301 tctattaatt cggaaatgtc ttttacttta tcggtatagg cttgatcaat aattttttga
     6361 gtaacgccag taaatttaaa taaattaatg aatgcctgtt ttggtattac atgtagaggc
     6421 aatttaattt taaagaaatc aattgtgttt attggaaaac caaaaggaga aactttcata
     6481 tcaaatgccc ctaatagttc ttctttttgc tgaggataag acagcgtttt aaatacgccc
     6541 gtactatcaa aaacaaccac tttctttgaa gacaataaat aattttctgc aataactttc
     6601 attgcaaata ctctgtcttc ttcttctgca ccggttacta caattgatct taggttctct
     6661 aaacttgcat taattgtaac accattcttt ctaccaacca ataacttatc tacagaacta
     6721 atggctgttg aaaattcaat attatttgga gaatttgtta atgccttaac aataaaagga
     6781 tcagaaaaaa aataattcct tatttctggc ggcagcatct ttagtgacaa taattttata
     6841 ttgtaagatt ttattataga aatgatggaa cttgtaattt tgtttaattt taacatcttc
     6901 ttctctatgt gctcattaat ttgagaagaa tctataattt cggtatctgt atttaatata
     6961 aaaaatttgt gtgtattttt atctccaata tcaaaatgct tttcaaaaac aatgcactga
     7021 tttggcagcg tttctgcgaa taactctatg tctccatcaa ccgcaaaaac cttgtataat
     7081 tgaacaagca cagaggatgg ttcttttgat cttaataaag aaacaaaata gtcatcatta
     7141 aataaaattg ccaaatcctt ttgaccatat acaccttcaa aaagcgtact ccaggttgtt
     7201 atttgtatca tgttattaca attgtggtta ttacccattc atattttaaa tcaaaacatt
     7261 taaataaata atatgttata tatttttaat agatatagta tttaaatgga ctttataatg
     7321 atagatgcaa cactaattaa agatacaatt accgaattgt tggaagcaaa cgtagataaa
     7381 gaaacaattt atcagacttt aaaagacatt ggtgtagaag attctgagat tgataaatat
     7441 tataaagaag caacaactaa agaaaaagaa caaaaaacag aaacaaaaaa tcctgaatct
     7501 aaacccgaat cacaccctaa aatcgaaacc gcatcaccaa aaattttaga aaatataaaa
     7561 actgaagaag caatacatca aaaaccaaaa gcaacaactg aagacttaga aaaggccaca
     7621 aaagatgtta ttgattcctc tattatggaa aatcaagaaa tagatgaaaa aataactaaa
     7681 gaagataaca cccagatttt agaaataaat aacacaaatt tagaaattaa aaagcaaata
     7741 tctgaactag aaaataatct tggcgatatt aaagcacaaa ttaacggcct tacaaaaatt
     7801 atgaaggaca ttctagaaga aaataggaat attctaaaca aattaaaata atactattgg
     7861 ttaccaagca tctttaataa atcttcgtag cctccacctt cttcttttgg cgctccgcct
     7921 ttcttaaaaa tggctacaaa aacaattatc aaaatacaaa ctccaaaaat aattaaccaa
     7981 aattgcggta tctccgcagt agttataaat aacattactg ctaacagaaa aactaaaagc
     8041 agatttatta gccctggaaa aaatatagaa taaagtatta caataactgg tgcaaaaaaa
     8101 taataattat attgaaaaga aaaaataata agtattccaa taacaaaaaa acttaaaaaa
     8161 tcaaacatgt tattttttta ctcctgccca accttttatt gtatttttta aatcgcttcc
     8221 aaatacccag tatatcccaa caccgattgc aactaaccag attaaatccg gatatctgta
     8281 aaataaaagg aaaactaaaa aaccagtaat taatattgca attaattcat tacctatttg
     8341 tttttttgcc caaacataaa tagaaataga aaaaacaatg aataatatta tccaaataga
     8401 atcttctaga gaggttacta aaataatact attcatttat gtctaccttt tttactttgc
     8461 ttttctatct tttgctctta ttgatggtct atttttttct gtgccttttc cttttctatc
     8521 aaggcctctg ttcttctttc ctgcacttgt tatgccttta tatgccctgc ctctattctg
     8581 tggccagcat atccaattta tgtctttatc ttgcataatc cttggatggt ttggatcaat
     8641 taaaattatc tcataatatt tttgttgacc atcttctcca atcaagtagg aatttaaaac
     8701 ctctagacca ggatattttg atgctgccct aagttctgct atcctttgaa tagatattct
     8761 tcttgttatc ttattaacac ccattctttt tggttttcta ccatttactg gtcttctaat
     8821 taaacctgaa ccttttctaa cttttgctag aactacaata aagccttgtt ttgctttata
     8881 gccaagctct cttgcccttg gaatgtttgt tggattatca agtctaatca ctgcttcttt
     8941 tgtatgtcta tacttgatta atctgtctgt atataatttc ttataattat aattatcatc
     9001 tttttctttt ttaaactctt tttgtattgt ttcttttatg tattttgatg ctgtactcat
     9061 atttactcca ttttttaatt ttttgcatgc attaatatat tattaattca cttataaata
     9121 atttataaaa caaaaccttt atatagtggt tgttgaaata agcaaggatt aagcttattt
     9181 tcaaaaaata cagatttaaa tgagaaatat gcatttattt tcttcaaaag aagccaaaaa
     9241 aaaagtaatt atcgctttag tgatatctct atttttagtg gttgctgcgg cggtttttat
     9301 ttggccatat gtttctattc ttactaagcc ttctgtaatt aaagaactgg tattaggttt
     9361 tggagtttgg gcaccacttg cttttattgg gctacagatt ttacaaatgg tttttttatt
     9421 tattccggct tcaccaatta ttattgttgg aggatatatc tttggagcgt ggggcgtatt
     9481 ttattctgtt atcggtatta ttttagggtc aataataata ttctttattg gcagattgtt
     9541 tggaagacct tttttagaaa gcgttcttga taaaaaaata ataaataaaa tagattatga
     9601 atcagacaat atcgcaaaga cattatttat tttattttta ataccgccat taccacatga
     9661 tgctttctgc tatatggctg gaattacaaa aatcgaatta aaaaaatata ttttagcagt
     9721 ggctgttgga agattaccgc aaattgtatt tttaacttta attggctatc aacttaccaa
     9781 attaaatttg ttttggtcct tattacttat aggagttata attgttggtt caattattat
     9841 ctttcaaaac aaaaacaaaa ttgaaacacg ccttcatggt tatgttggga aattacagaa
     9901 aaaataaggt cctatggtct agtggttatg acgttacctt gacatggtag agatccccgg
     9961 tccgatcccg ggtgggacca tttaattttt ttaggggaag agaaaaaaat gatatcaaaa
    10021 cttattgctc ttttagttct tttttttgtt atcattgatc ctcttgcaag ctttgctgtg
    10081 ttttttgtta ataccaaaaa ctataagaaa aaagaaaaaa taaagattgc aataatggct
    10141 gttgccattg ctgcggttct ttctttttta gtattgttat ttggagaaaa cctacttaca
    10201 atttttagca cagacataaa tagctttaag attgctggtg gaataatcct actaatactt
    10261 ggaataaaca tgtctcttgg aagatcgatt gttgaagttg aaaaaagtaa tgcagactca
    10321 gttcatgcaa ttgcttcagt tattgctacg ccacttctta ctggtccagc aacaattact
    10381 gcaataatta tttctgcagc agactatggg aaagtcctaa ctggtgttgc aattggaata
    10441 atcttactgt tgactggaat atctttatat cttacaatta ttttccatca cttgacagaa
    10501 aaagcaaaaa ccacaataca aattattagc acaatgcttg gacttattac tcttgcttgg
    10561 ggagtaaact atatacttac tggtgtgcaa gctatatttc atctaatcta aagtagtttt
    10621 ctttgaaaag catctttaac aatattatct attgtcttcc atttcattcg tgtgtattct
    10681 ggaaagcttt tgtacttttt aaaatagtct tttagaaaat ctatggttct tgggtcagaa
    10741 gagtatcctg aaatatcaga acctactaac tcttttaaca ttttatctct tgtaactttt
    10801 gcaagaattg aagcacacga aacagaaacg tggtttaaat ctgctttgtg ctcagaaatc
    10861 actttgaatt tataatcgca agaaattgct tttagattat cttttattct cttggaaaaa
    10921 tgatctgtag aagtatctgg agaatcaaat actacaattt ctggttttac ttttagttta
    10981 gaaagagcaa aacaagcctt ttgtgcttct atttcgttaa gggaaaatct gtccattaaa
    11041 acattaagtt cttcagcagg tattgcaaca atcttatagt cttctgcttc attttttatt
    11101 agttcaaata attcctctct ctgctttggt gagagaagct tagagtcttt tactcctgca
    11161 tctttaaaca tcttttcctt gtttttatca atcacacaaa cagctaagag taaaggccct
    11221 aagacgcagc cccttcctgc ttcatccacc ccgctaatca gttgtttagt catcattaat
    11281 caagcttttt aaatactttg tttattaaat ataatatagt acttatttta aagacttatt
    11341 aaattggtga aaatactatg caaggaacga taatgaatta cagaatgggt gtaaacacac
    11401 aaaagaataa tcatttactt ataagaatag atgatgaaaa atacagaact aaagagtctg
    11461 ttgctccatt atttggacat aaagttgtat ttactactat tggaaaaaaa gtaataactg
    11521 gtaaagttgc ttcagctcat ggtaataaag gaattattag agttatcttt gaaagaggac
    11581 ttccaggaga tgctgttggt caaaaagtag aaataaaata aattatgaca aaacataaag
    11641 atttattttt taaatacaca atttctagac taaagacaga attagaaaga gcagatcaac
    11701 gaataattgc tatttctcaa attctttcta gtttgcctaa aaatataaat gagatatttg
    11761 aggaacttag aattattgga gatattcttt atcctaatat ggaaaaagaa ctaacgcttg
    11821 aagaatatgt tagactttgt tctcttgaag aaatttcaga aaacacatta aagaaaattt
    11881 ctctggataa aactaagata agaaagattt tagaaataca agagatgggc ttatctgcag
    11941 aattagaaaa agacgaaaaa atcatattta ttaacttagc aaagaacctt caagcactac
    12001 ttgataccaa gaaaagcctt gaagccttag aagaaaaaat ggtaaaaaca aattatccta
    12061 acttctctga gattgcaacg ccagccattg cttgtaagat gattgaagtt gctggttctt
    12121 ttgaaaggct ttctagattt ccagcttcaa ccatacaatt acttggtgca gaaaattctt
    12181 tttttagggc gcttaaaact cgaagcaaaa aaacaccaaa atacggtttt atttatgctc
    12241 atcctttgtt aataaactta aacaagaaag acaaaggcaa gtttgcaaga actatttctg
    12301 caaaaattgc aattgcaatc aaggccgatc ttggcggaaa agacctaaga aaagaattaa
    12361 ctgacaaaat taatagtaaa ttaaaaaaat aaagaaaaat tatggcaata aagaatttaa
    12421 taaaggagaa tggagatttc tttaccttag acttgaacaa taaagagaag agatactggg
    12481 acccaactag atctaaggtg tgtgcggctt taaaaaaagg attaaatttc ttgcctctta
    12541 aagcagactc aaccattctt taccttggtg cagcagaagg ctatacttgt tcttatattt
    12601 ctgacctggc ttttcaagga attatctttg gagtagactt ttcagctcac agcatgcaaa
    12661 agtttgtttt gctttgtaaa gaacgagaaa atctttatcc aatacttgga gatgcaaata
    12721 agccagagga ttataaacaa ttagtagatt gcaaagttga tttaataatc caagacgttg
    12781 cacagaagaa ccagattgaa atacttacaa agaatgcaaa agaatttctt aagccagaag
    12841 gatatgttat tctaagtctt aagacaactg ccatttcaca aaggaacaca ggggtaatca
    12901 ttgacgaaga aataagaaaa tttgaaaaag agtttacaat agttgacaaa agaagactag
    12961 acccttttga aaagaagcac atgattatag ttggaaaaaa gaaataacta ttttattccc
    13021 ttggatttta gaaaggcatc tattgaccta tcataagtct tttctgcctt tgtatcaaag
    13081 taacaagcag gtagctcgcc tctctttcta tggtaagaaa cacattcaca gcagaggcct
    13141 ttcttctcac aatcatatgt gcaattgcaa gacttcttgt ttatttcttt tttgcattcc
    13201 ataattaatc acttcaaatc ataaaaagaa caatattaaa aataatgtaa agcaataaca
    13261 aagcaagacc ttcttttttt gtaagctttt tatcaattat aacataaata aacgaaatca
    13321 aggctaaacc aaacattggt attgcaatct tgaagatatg agacggaaca ataacaaaac
    13381 caaagaatct agaaaagctt gaaacaatta aaatatcaat aatgcttgca ccaataatgt
    13441 ttgctactgc aatgtctaga tggcctttct ttacagaaat tagaccaaca gtaagttctg
    13501 ttaaacttgt gcctaaagca ataacagtta ttgcaataat ttctgggaac accccaataa
    13561 ttcttgaaac attaagcata gaatctacta cgtattttga accaacatat atcaatatcg
    13621 aataaattaa aataaaaaat acacaaagat aagaattaga ttttgtaggg tcttctaact
    13681 ttactggttt tgataattta tattgataaa tagaatatgc taaataaaga attacacaca
    13741 aaaatgcctc taatagcgta aaattaccat caataataaa taaaacaaaa aggcaaacaa
    13801 ctatagcaag catccataag tcagttttta atgtatcttt gtaaatgtat tttgtgccat
    13861 acaaaaaacc cacaagacct ataaccaaaa aaatgtttgt agtaactgac ccaataattg
    13921 tcgggataac aatctctgat tcccctctta aagttccata aattgaagaa gcaagctccg
    13981 gtagcgttgt tccaacacta acaattatta agcctattaa aaatgtagag agccctagat
    14041 tcttaccaag ctcttttgat ttatccacaa acatatctgc agagaatatc aatacaaata
    14101 aacttgtaag aaaaactaaa atccaaaaaa ttaaagcaaa taacataagc ccctcatttc
    14161 ttcacatatg cttttgttat tattatgtct tctattggcc aatcttcgtg accgccttta
    14221 gttccggtct gtacgccttc aattgcttta actacttcca taccagaaat tactttacca
    14281 aaaacagcat aacctggatt acctggtgtg taatttagaa aatcgttatc tacagtattt
    14341 atgaaaaact gagaagttgc tgagtttgga gcatttgttc ttgccatagc tatagtgcct
    14401 tctgtgttgt atagaccgtt atttgattct aagactattg gactattggt gtctttttga
    14461 acaccagata ttgtaaagcc cccaccctgt atcataaagt cctttattac tctatgaaaa
    14521 actaggccat cataatgacc agaattaaca tatgctaaaa aattagcagt agttattggt
    14581 gcatgatttg tatcaagctc gatttctata ttaccttttg ttgtttctaa aattactatt
    14641 ggaatcacat tcatattttt atcctttttt aatattgaaa atatattgat tagcaaaaca
    14701 attaaaatga ttgctaaaat tacaaaaatt acaattaatt ttttattttt catgcattca
    14761 cctttttctt ttctaattta ttaacaattg gaacaaaata attttctata acaaaactta
    14821 cattatcaat aatctcattt aaggttttat ttattatttt ctcccattct tttatttctt
    14881 tttctatttt ctctatattt tctttatcat cagaatgtga aataacataa agccttaact
    14941 gaaaaacaaa atccgaaaga ttatctttca aagacattaa ttgatatctt aattttatag
    15001 tttttttagc actatcatat acaaatcttg gatggctgtt tttcttgtat accccggaaa
    15061 caaatggatc gattatatgc ataaatttag ttttaatttc tgtaattgta tccattaaat
    15121 caactggctt ttttattggc tttgttttaa tatctagagt cttatgaata ttcttaatat
    15181 ctaaatctag ctttggctca atctttttta agtggcctgg cttttttagt cttatttttt
    15241 cgcgatattt aaagggcata attattccat tttagcctct agagggactt gaaccctcga
    15301 ccttttgctt acgaggcaaa cactctacca ctgagctata gaggcaaagg caatctattt
    15361 tatttttaaa aactttgttt taccatagta aagcttttct aataattttc ctagagactt
    15421 tactgaaact cttttttgtt cttgagaatc tctatctctt atcgttactg tttgcttttc
    15481 taaagtttcg tgatcaattg ttaaacaata attacaacct atctcgtcaa ggcgcgcata
    15541 tcttttacca atagaacctg cttcatcata gaatatatca tatctctttt gctcttcttt
    15601 taaagcatta aatatctctg ttgctttctc agaaagcccg tcttttttca ttaatggtaa
    15661 tatagcaaca aaatacgggg aaacctttgg attaagatgc aaaatgtttc tttcctctcc
    15721 atttatttcc tttatttcaa aagaattttc caaaagaaca taaaataatc gtccaagacc
    15781 tattgaacaa acctctagaa catgtggttg tattcttttt ccgtcttctg aaattgaaag
    15841 atctttcttt gaacctttac cgtgagctaa aagatcatag tctgttctgt aattaacaca
    15901 aaataattcg acccattctt tttcggtttt tacttcacaa tcaaaggttt gtttagaata
    15961 aaaggccttg tcgttatctg gtaattctct aaatcttatg ttgtcgtgag agaagccaag
    16021 atcttctaag aacttttgag taatataaag ggtgtatgta attagattcc ctgatgttat
    16081 ttttttttct gtaatttttg aaacattata gtccttttct tgcttatctt ttaacaactt
    16141 aaatcttatt ttgtattttg aaattgtgtc taatggaatt ggtgtttcat ctattttgtc
    16201 tgaatcaaaa aacatttctg tttctaattg ttcaaactct ctacatctta aaagaccatt
    16261 tcttggagaa atttcgtttc tatagacact tccgtgttgt gagattgtta aaggcaaagt
    16321 ttgtcttccg gttttatata ttcttaaaaa atcaacaaat attgattgac aagtttctcc
    16381 tcttaagtaa cctaaattac ctgattgtgt gccaacatta atacttgaca tcagattgaa
    16441 ttttttaata tctgttaaat ttcctttaca tttagaacag acaatattat tgtcagcaat
    16501 taatttttca tattcttcaa ttgacatact ttctggaact tctttttttg tgttttctga
    16561 gattaatctg tcaagtctat aaacggtttt acatttttga caagtagcta ttggatcatt
    16621 gaaattttta agatgacctg atgcttgaaa aacatcctct ggaagaatta ttgaaccaga
    16681 aatttctata aaattattct ttcttacaaa ctcttctctc cagaaattta taagatttat
    16741 tcttatccta ttccctattg gtccaaactc gtagaaacct gcaaaattat ttgcataaat
    16801 ctctgctgat gggaaataaa gagaacgagt tgcacaaaac tcattaaatt tttccaaacg
    16861 tgtagccatg attttcacta tttcatatta taaacatttt gttttctatt atattatata
    16921 gtaatagaat aaaaacatat ttaaaaatgg ctttttttct atttatttaa cattagatgt
    16981 gtgtcagtaa acactattgg gtgcgagctg accgaccatt gcgcgctaac cgagcgaaac
    17041 cggttattgc aggggttccc gagcggtcaa aggggcatgg cttaggacca tgtgcttagt
    17101 gcttcgcagg ttcgaatcct gccctctgca ctttttttaa aaaactagta tctattattg
    17161 ctttctatta tctttttcat tttctcaaca cgataatcaa ttcctttcgt gtccccaaat
    17221 tttaattgag aaaacttact tttgatgatt tgatattcat taaaagaatt aactaaatta
    17281 gtcaatattt tttctctttg cttatcactt aatttattta aattttttcc ttcagattct
    17341 aatactcttt cataaagaga ctgaagttta ttcccaatcg ccctttcaat aaaactctgg
    17401 gcagttcttg tgtcttgtct aaacttttca acagcttctt caaattttag tattggctca
    17461 tttactttct ttccggcttt tatttcagaa atataatcta attctgcatt agccattgct
    17521 tcaaaatgtt gtaatacatc ataattttta ctaacttctg taaatttttc agaaagtaaa
    17581 gcagtgccta atttcatttt ttgtacttgt atttttaatt ctcgtaattt atttgcatct
    17641 ttaagtaatt tggtctttac tttttcagga attgctggtc tttttacaat ttttccgtgt
    17701 tttttctttt tttcagcttt ttcttttggc tttggcctaa cgcctgcaaa ttttggcata
    17761 atctttccct cttaaatatt attaatttat attaacaata attaaataaa agatatttaa
    17821 aactatcttt ttaacagaaa ctaattaatt atttataaat ggcgccagtt tccatatacc
    17881 aaagataaaa atcaagctca ccaagagtca gattagtttc ttttgctagg ccttctaatt
    17941 ttttctctat ctttaaatat gcgtctttgt taagattttt tggctttttt acaatcttat
    18001 tgtctgccaa gatatcgact atatgaaaat caataattgc ataatcgaaa aaaccaatgt
    18061 ttcttagaaa atgactagat tccttaaagc caaggccctt gatatttttt acaagccact
    18121 ctcttttttc gtacttgtct ttaatagaaa aaagtttttc tttaatgtcg tctttgtgct
    18181 cctgagccag aaaaataaaa tgagcacgcg tgtttggaaa cctgtgaccg tttgctttta
    18241 gaagctttgc aagttgggct tctgtacctg taagaaaaag acaaccacac ttctcttgaa
    18301 tctcaactgc ccttttagca ttaaaattgg cggtcatcaa acaaaagcaa agctcttgaa
    18361 atatgtcctg agaagatttc ttggaaaaac ttttaaattg ctttatcttt ttagaaatag
    18421 ttttttctat tgctgttttt tttaaagcat taatttgaga aacgagtttt ttcatatctg
    18481 tccaagattt agtgtatttt tagagaaaag aagtcattta agacttccaa actccgtgta
    18541 tattacatag acatcttgca cttatcttat agcccccacc tttaaactca gcaacaggct
    18601 catctcctgg agttagtttc ttaacataca ctgaaccatc aatattaact tcaatccatt
    18661 tgatgtgatg ctctgctgtc attgggtgtg gtatgtctcc aactctaaca acaatactat
    18721 ctcttgtttt ttcaattact ggaatgtgct tttcctttaa ttgttcttcg ctagtgtcgt
    18781 gatgaagatc catgttttgt ccacagcaca caagcgttcc gccgccagca tcaattactt
    18841 caacaacgtt accacagatc gcacatctat aaatttctcc tttttttacc atagtttttt
    18901 acccccttaa taattctcat tttgtactct aaaatactct ggtggatgac cacatgatgg
    18961 gcattccttt ggtggttcct ttcctttgtg catatatcca catttactac atatccacac
    19021 gacttctatt tcttttttca ataacgtttt ttcatctaaa ttctttaata attttttata
    19081 tctttcttcg tgatgctttt cggcaattgc tattgctcta agtttgtttg caatttcggg
    19141 catgccttct ttttcagcga catcagcata ttctggatac atacttgtat attcgtgatt
    19201 ttccccagaa attgcggctc tgagattgtc tgctgtattt ccatatgctg ttggaacatc
    19261 tgcttcaaca actagatctt ctgtaatatt attttgtttc tttaattctc ttattaatac
    19321 aaacaattgc tttgcgtgtt ctctttcttg ttctgcggtt tctaagaaga tatctgcaat
    19381 ctgaaataag ctttcttttg ttgcaacctt tgcatagaaa gtatatctgt ttcttgcttg
    19441 agactctcca ataaaagcct tacatagatt ttctatagtt ttcatatatt ttaccccctt
    19501 tatcctataa atatataaaa gcataaatat taaatatatt gttataatat atctattgta
    19561 aaaaagtgtg aaaaagatga ctgaaaaaat aattgaagaa ctaagtcaaa taaaatttaa
    19621 agcaacaacc atctatgagt cctttaaata tcctttacat cgtacaaata aaacacaaag
    19681 aattcctttg ttaaaaaata taaagaataa tattgaggaa ttaactagac tattaaatgg
    19741 gcttttaggt tattcgtcta tacaatttaa taaagaaatt gaagcagaaa agatcaggct
    19801 gttccaaaac gtatttctgg aaatatcagg taaactacaa caatcaatct ttttagaaga
    19861 acacaccaca attgtagaaa atgatcttgt aaatataaaa aatgaaattg caactcctga
    19921 gaaatacttt gaaatagaac aatctgttaa aaacaatata aataacctta ttatttttat
    19981 tgatgatgca aaaaaacaaa ttgatcacaa atatgttagt cttgcaaatc aaaaaagatc
    20041 tgttgaagag ttattaaaaa cacttgaaag aaaagatcaa acaatattgg aactaaacaa
    20101 aaggattgat gaactcaatt ggatagatgc aaaagaaaaa gcaaaaactt caagacttgc
    20161 aaatttagaa gaagaattac ttaaaaccta taaagcaaat gaacaggatc ttaccatttt
    20221 aaagttgcac ataatacata ttgaaaaggc cctagataat cttttaagag aatcaaaatc
    20281 attaactcaa gatattaatc atctagaggc aaaaaacata ttaaaagagc aagcatcatt
    20341 ggaattgatt aaagaattaa aaaaagaatt gattgcagca aaatataatt taaaaaataa
    20401 gtgataaata atgtctgaat atgatccatt tggcggtaga tttccacaag actccgaaaa
    20461 accaaaatct aattttaaat tagatgaaaa aacaattggg ctaattaaga aagtaattat
    20521 tttagtcgtg gttttaggtg ttattggggg cgcgatttat tttttctttt ttaataacat
    20581 tataattaat ttctctgtaa aagacacaga agggaaggca ttaaattctg caaacataaa
    20641 aatcactcct gagaaacaaa ataaatcaat tcaatattct ccaggagatg aaataaaatt
    20701 aaataaaaat aaaaattata aatatgcaat tgaattaaaa ggatataaaa caattaatgg
    20761 gcaattagct cctaaagaca tcgtagaacc aaaccaggcc attaaattag aaaaagacat
    20821 tagactagat gttctttctt ttatttgccc aacagacgta tttattgggc aaactgttaa
    20881 atgcgaatta caattaaata acattagcga aaatgaggac tataatctag aaaatttaat
    20941 ttttagagac gataaagata aaccgcttaa aaactgggct gatttaaatg atgaattctt
    21001 aagatttgta gacggatttg atgcagaagt ccctagcacc aaaataatta cacacaaaac
    21061 taaagacact gtttttattt tatttagtgt gccagaaagt attaaacgcg gaacaacaca
    21121 aaaaattatg gcaagaatta agtacacaga taataatgca attcaagaaa taaaaatatc
    21181 tcaagcgccc aatatagggt ttacttctga actgagtagt gctttaaatt tagaaagcgg
    21241 aaatgaaata attaaaacat atacaattga taactctaaa aataaaacag atctatcggg
    21301 attagaacta gaaatcgatg caaattatga gcccacaact ggagaattag attctaattt
    21361 cgatatagcc gaaataatag tgccagatta taccacccta aatgtaaatg catcttcgcg
    21421 ttcacaagga caaataacta ttaaattacc taataattta agggcgggaa aaatcacagg
    21481 aattttagca ttaaacagcc ccattttttc tgaaccaaaa aaaatcacat ttacaattaa
    21541 tataaaggaa ccagaaaata aatttaatat aagtctaaat aaaagtacag aaacgttaga
    21601 ttatgatgca aacactaata ttacaactat taaacagata actttaaatt tagataacaa
    21661 gaacaaaatt ccagttcata taaattcaat ttatattgaa aactcaacag gaacaacaga
    21721 ttgtaatcat tggatagttc ttcctacaga atataatggt tatgacattc aatcaaatta
    21781 taatccagcg cctataattt tattacaagg atcggaccta ggctctttaa caacaataac
    21841 tggattaaag atttgtaata ttaatgtaga ttatagacat ccatatttag atgaaaatat
    21901 aattattaaa aataagatac aaataagtgt aaattaaatg gactatattg attggataat
    21961 ttctagtgtt ttatttatta cagttattgc atttgtactt attagtttat ttaatatttt
    22021 acctaaatca aacaacacaa atacaatcag tgatttacta tataaatcta caatctctct
    22081 gattattccg gtttataatg tttctatgat ttctgaagat tcagaagtgt acccttatgt
    22141 tatttcagac tgtaattatg gtagagcaga aaacacattt gaaatcgata caaacaataa
    22201 tttgttattt ggattgattt atgataaaga taaattctat aattatgaaa cagacattaa
    22261 tgctttatct ggtcttggag tacgattatt attagaaaac tttaatgatt ataattatca
    22321 agacactttt gatttaaatt caggaaccgc aaatataagt tctggaattt tagagctaga
    22381 ggccggttca acaatagaca caacaataga attttctaaa tatcttggtt catttgtaac
    22441 aaatgcagaa gatataaacg tatatattaa ttttgatact acaagcgaca gattttattg
    22501 tagttttaat gaaacaggcg tccagctttt taaaattgga gaatcagaag caattgaaga
    22561 aaataatact actctaaaaa cgacaaactg gagaaaatta aattttggat attacaggga
    22621 ctttgaggga ttttcctggt ttttttgtgg aattggcgaa aacattaata tcctagaaac
    22681 ctctagccaa acacatcccg aagaaaccac aataagaatt gaagcaatag atgatcaaac
    22741 ttatatagat gattttaggg tctataaaag tttaattaat ataacagaac atgatggcac
    22801 tacagttaaa acagattcat taagaacaac agtaactagc gcagatatga gagtagaatt
    22861 ctatgaactg tccgaaatgg atgctaatga agtattgggg tgtgctctgt ctttttccac
    22921 ttcaagtgga aatttaaata attataatga ggggccaatt aaaattttct caaatgattc
    22981 aaatcaaaac aaattagttt tctttcctat agcaaaagaa ttttggatat ataagtatac
    23041 tggagaagat ccaattacta ttacttatat agttgatgaa attaaaccgt ataattttgg
    23101 ttctgcaaca gaaaacgggc aatttgtaga tttaaataaa ataccaggaa gctatgctgt
    23161 gcctgtttcc tttgttcctg aaaattcttt tacaacagaa aattttggtt cttttgatgt
    23221 gaactttaat aaagtttttt ctgagattac gccaacaggc ggtggttctg aaacgccaat
    23281 tatacaatat tgtaaagttg agggatgtag cgatattacc agtgattcta attttatatt
    23341 tgatactgat acaaacacgg cagaagtgat ttttaaattt ccggaagaaa ctgttgctaa
    23401 tcaaccattt aatattgtta ttatttttaa ctattttaat ttagaaaatt tagaaaatgc
    23461 taccggagag gcaacggtgc ttgatggcac acagcttact gcaagggtct cagcagaaga
    23521 aattataatt gatacaaatc aattttattt atataatatt tttaatccta tttatccaaa
    23581 cattaaacaa acaataatat ttgatgtctt tgataataca ggattacatg cacaggattg
    23641 taacttcttt ataaattggg gttctcaaaa tatagttgta acttgcgatg aaaattattt
    23701 acttaaagtt agatatagat ttactgattt aaataatacc attttagatt atccaaatct
    23761 atcaatcaca aagactaacg aaagaattgt gacacaagaa cattttgaga atttatcttg
    23821 tgattcttat tttgtaaata ttaaaaataa aacattagat gttaactgtg gtattggagt
    23881 taatcctaat tataaaatga ttataaaata tcttcataat acgggattat tagaaaatgc
    23941 agacatatat ataaaataaa aagaacaatt tctaattaga ttattgctct aagaatgagg
    24001 ataacgatag caatacctaa agataaacct attactgctt ctggggataa ttgcggacct
    24061 gctacagact ccttaaattt cataattcct atagctgaac tgggaccttg attattattt
    24121 ttagaataat tcatcttcat aaatttatca caatttaaat agaactatat atatttaaag
    24181 aaaccaaaac cttttatatt aatctatttt atattttatt agggttgaag ggtatggtag
    24241 agcagaaaca atcagaatat aagtggctgg taagaataat atttctttta ataattatag
    24301 ttgcggcatt atttgtattt atttggacag gaactataag gtgcagatct atacctggcg
    24361 gctgcaacgt gtactggagc gttatgacta tagttacagg aaaacaacaa ccgagcgttc
    24421 taattttata tgaccctaca gatcagaaag gtctgggtaa tcccgaacta ttaaaagatt
    24481 tgctgactga tcgaaaaggg gtggcaatac acccttccct tgcggatatt cgatacctta
    24541 attctgaaca attaaagaac gtaagtcttg tagttgtaga aaaggcaaga aaaatttcta
    24601 cgcttcaatt aatgactatg cgaaactata tcgcacaagg cggaagaatt gtttggattg
    24661 gagatgcagg aatagaacac aatgaagata aaggagattt gctatatgtt ggcccagaaa
    24721 acgaaaaaaa aaatggatgg gtcagattaa cagatgcaaa tatgattata ttttttaaca
    24781 gaactctagg ggttaattat gttgcaaatt tttgcgatat aagagattgc gatgttgggg
    24841 ataaatttac tggaaagtta atgccaagtt cagacactca tccgttagtt tatggtttaa
    24901 aaccaaatct cccaatctct gataattatt ctatagtgac cttgatagaa cctaatcccg
    24961 ttcctttaaa aatagatttt ggagcaaatc tgcgagatac aaataataac ggttacggag
    25021 acatatttga tgccatagtg accagcaatt caaatagagt ggcttactat gctatccccc
    25081 ctgaatatct tgcagaagac aaagatggag aaaacaaata tttttctatt atttataata
    25141 tgtttgaggg aatggtaaat tgacatgaca aaatcttttg aagtcgcaat ttctatcatt
    25201 ttgcttttat tttttgtttt ttttttattt gaaactataa atcagaaata ttctcaaaat
    25261 cctataccag aagaagttaa aaccataatt ttattagaag ctagagattc gggatttagg
    25321 gaacttatct ctaactctga agttgataat gtctattctt tactttatcc gcaaatggat
    25381 tatagattta atgtaagcat ttgtgattgg ttagatgata attgtgaaaa tagagaatta
    25441 gagaatttcg caaaaaaaag agaatttgtg tatttctttt ctgatactaa taaaaccctt
    25501 catattgaat tagattaaag gagcaataat ttgaaaaaag gatttacata cattgtaact
    25561 acaatgttac ttataatact tataagtggg gttgttattg tatctaatta taacgcccca
    25621 gggaataaaa taaaccctgt agcaatactt gcaaaaaact atgaaaacga gttcttaaat
    25681 attttaaact ctggttcatc tgttgaagac attaatcaat ttaattatag atttaagaaa
    25741 tttataaaca gcaataatta tgattctaaa ttgtgtaatt taattactac agagaatggg
    25801 attattgcaa gcaattttat ggaccaaaat tgtgatttaa tgataaatgg agtatctaat
    25861 caaacaatta cagagaataa cacaattata acagatatat ttataaatga tacgaatata
    25921 tatttatgta actgttatta taaggcagga gagaatatct attatataga tatttataat
    25981 gagaactcaa aaactacatt taaaaattag aggggatatt atgaagaatg taactgaact
    26041 attaaatcag atagaagaaa gaactaacat aattgttgca aaattagaaa ttttaagatt
    26101 tgcagaagaa gatgttcaga caattaaaaa atcattagaa gtaattaaaa agaaacttga
    26161 atctggagaa ataagtaaat tttcgtatgc cacaatgcta gaaataaaca aaaaaagagc
    26221 aatggaaaat accaattcca aaaagaaaac gtgggatgaa gttgcagaat ccataaatga
    26281 aataagcgat ttactaaatg acttaaaaga tacattcaat gctaaaacag aatttgaggg
    26341 aatagatgaa attaaaacat caaagtctta aattattatt taaaatcgaa tataaagatg
    26401 agccatgaaa ataccaaatc aaaaggatgc atcaaaactt tatgacctaa ttcttaatct
    26461 taaaaatatc gaaaatcaaa gaactgcaaa agaacaagaa atattaacta aattctataa
    26521 tcaagaacta gattctaaaa aactagtttc tgaattaaag aaagaaaaat tattttctaa
    26581 ttctaaaaaa ttagatttga attttattaa cacatttatt gaatatagca aacaactaga
    26641 atttctttcc gaaaacatag atgtaactac tataaaagaa tataaattaa ctaaaagacc
    26701 agcacctttt attttaggat taaaaaaaat attttcttca aaaactacca aaattagcga
    26761 aaaaaagaaa tctgcaaaac caaaattagt ggaagttaac ccaaaagaga aaaaacaaca
    26821 acaaaatata ttccaaagcc tttttaaaaa tcttaagaca gataaagaaa ccataaaaat
    26881 taaagaaaat gctaatccgt tgccttcttt tgaagaacca aaagaattgt taccagagaa
    26941 ctatcaaata ttaattgagg gctatgccta tgcatttgtt gaagaaaaag aaggttccag
    27001 aagatatgtt gttgcagaac ctgaattaac ctcagctgaa gaaaaaatac ttaaagagac
    27061 aaaacaagaa ttaataaata aaatatcact tattgaaata aaagacgaat ctgaattaat
    27121 aaaaagaata gaaaaaatat ttaataaaac aaagattact cctcaacaaa aatcaaaaat
    27181 aatgtattat gtaggcagac agattaaagg gctagatctt atagaaccat taatgcacga
    27241 cccgttaatt gaagatatag aatgcgatgg aacaggcatt ccaatattta ttgttcatag
    27301 aaaatatggc catcttgaaa ctaatattgc atacaaagat gaaaaagaac tacaacagtt
    27361 tgtaattaag atggcgcact taagtaaaag ctatgtttca tatgctagcc ctttacttga
    27421 ttctatactt cccgacggtt ctcgtgttaa cgctacactt acaactaacg tttcaactag
    27481 aggcccaaca tttactataa gaaaattccc acacacccct ttaaccgcaa ttgatttaat
    27541 tactagcggc acattaaacc ctatgattgt cgcgtatatt tggacaataa tggaatttca
    27601 aaaaactgca ttaataattg ggccaacagc tggcgggaaa actacattat taaatgttat
    27661 tacttctttt attcctccag gaagaagagt tgtttcaatt gaagatacta gagaaataaa
    27721 tttactttta gataactggg taccacaaat aactagaccc gggtttggcc cccctgatgg
    27781 aactggcaaa aaatacgggg aagttacgat gcttgatctt attaaagaat catttagaca
    27841 aagaccagat tatttaattg taggggaagt cagaggagaa gaaatgtcaa tcatgtttca
    27901 gggcatggca tctggtcact gttgtttgtc tactgttcac gcaagaagcc tgcctgacct
    27961 cgtaaaccga cttataaccc caccaatatc tcttgaacca gcactattaa cttctcttga
    28021 ccttgttatt gttactggat ttgaaggtgg cagcgacata aaaagaacaa taaaagaact
    28081 agatgaaatt aaaggattta attctaaaga aaataaaata gaatatacgg caatatataa
    28141 tgtttatgaa cgctctcaag aaaaagacgg ggatttattc acacacgaac tgccaattac
    28201 ctataagagc gaaattttac aatctattgc tagagaatat aatattaata taaaccattt
    28261 attagaaatg ataaacaaaa gaatcaaatt tgtagaagaa ttaaccaaaa atcccccaaa
    28321 agactatgtt gaatttaaaa ttgcaataaa taaatataaa ttgcaagaaa aagtaattac
    28381 taattagatt tatttaaaag ggtctcttct attaaacaag tattctggat ctatgtcctt
    28441 tgattttgtt ttttgttttg gcttattttt tattttgatt ttctttacta aagttatact
    28501 cactagaacc acaattgttg caacaataat ataaataaaa tattttgtta agtcctgagt
    28561 ttcaatctca ttaatccctg catcctttat tttttttact tgattttttg ccagttttat
    28621 cttgcttata ctctcttcat acctgccttt attatataat tcttttattt gagcataatt
    28681 ttgacttaat tcagatacag tttttgtgtc tttccataaa tttatatttt ctgaatttag
    28741 tatttgattt atggaattat ctagtgtgcc ttttaaatct gtattaaaat tatatagcaa
    28801 agattttgca acataataat tttgtctagt ctctagagtt tgttgtaaga tctctaaggg
    28861 actattgggc cctgcttttt caaaatcagc aatcactaaa gaatagaact gatttgttga
    28921 tgcaaggcta tctgttatcg tagaaatgtc cccgtttaag ttatttggag cttcaatata
    28981 gctcaggatt atcttttttg tggtttcatc taatttttga tctgcaacta taacttcaac
    29041 aatctcatta gttgaatctc ggtaatttat agaagatgaa acaatatcgt tatacccctt
    29101 aaacgtattt ttatataata atatcaaact atctgctttt gtacaatcgg tagtatctaa
    29161 aaacgtagat tgttcttttt gtaatttact aagctttgtt ttcatgtctt gtaattgatt
    29221 aattaataat tctaatttat cttttgtgtt ctgacatttt gtagttattg aagaagaata
    29281 atttgatcta ataattccgt ctaattttgc ggattttgat tgcaaggtta tatttaaatt
    29341 atctattttg tcaactaatt gctgagacaa atagttgttt ccacttttgg tttttatatt
    29401 tctattaagt aaggccgttt taatgatatt ttgggtattt gtatttattt cataaataac
    29461 agacccatta tttgattcta taggcaaaaa taacacaata tttgattctg catcaacaat
    29521 atttagtgta taatactcgt tattatcgca atcaatatag taaacactgc caggatctaa
    29581 atattcatta tttgacaaca tatattcttt agcaatatcc tgtgccgaat ataaggaaat
    29641 ggcttgtgcc atcggaatta taaacaacaa aaacatgaca gcaataattt ttttcattct
    29701 tcctcttctt taaatatttt attgaacaaa tatactattg ctgcaaatat taaaaaaact
    29761 attgataggc cagtataaac gaaagaagaa aatgctaatg caaagaaaaa tgcaaaattg
    29821 ctttctataa aacccataaa tggaattgcc tttattgtgt ctatgatttt gactttaata
    29881 actggcgcat caagatctgg cattagttct tttatttctg cagttgtttt atttgctgct
    29941 gcagaggttt ttagattttc cataaaatca gtaaaatcag aagaagtttc atctgttttt
    30001 attcttaatt ccaaatattc tctagattcc ataatatatt ttaaattaga caaagtgcac
    30061 tgctggttta ttatatatga ggtgctaatc caggggtcaa tattatctgc aataaataga
    30121 tttacaagac ctgcttcgaa ttcttctact tttttatttt gctctggaag aatatatatt
    30181 gcagaagaaa tgaataatcc aattattaaa aatatagtta atttagataa ataatctgtt
    30241 gtttgattaa acaaattttt ctcagatgtt tttttaacta ttaaacacaa aataagatgt
    30301 gctgatatat ataatattag aaataaaata aaataaatat taagctttaa aaatataacc
    30361 gaaaaaataa aagacaaaaa taaaccgaca ccaacaatga ttaaactttc tttttttaca
    30421 gaattatata tgattgataa aataattatg gaagttatag gaaaaaatat cacaaaaaat
    30481 agaaaattta gagaaaatat agaattaagt attgtcgcta tatcaaatac cctgaaagca
    30541 taagccgtta acttagaaga aatcaaaaaa taaatccaaa aaaatgaaga gaacgtaaca
    30601 attaataatt ttattagatg tttagaatta aattccattg cacctttttt atttatcata
    30661 aattccacta ttcaattatt tcatttatat ttattagatc tatcatttct tgattttctt
    30721 tatcataaac aaatgttgtt tctgcttgag aaacataaga gccatttcta tcaattaaca
    30781 caggatatgc agatattgcc tgcgctttta atagttctct aaatgcaact ttcctatcaa
    30841 agcttgacag gtttttccct acccaaaatt cggaaaatgc cataccttca aagcttttga
    30901 tttcttctag tatttttctt gcatttgagt ttcttattga aacatctttt tcaaaaataa
    30961 agatatagac gttgcttcct tctgtcacat atccattacc agtactagag aatggttcta
    31021 ttgcaaacca agaatcaatt tctgataatt taatattaat attatttttt acatttggta
    31081 ttgatgggtg gttgtgagtt gtatatcttg taatggtgtg gccagttaga ttacttattg
    31141 gcttataacc ataacctttg attgtatctt ctataatttc cccaactttt gaaacagtta
    31201 caattttagg atttttaaga atataatctt ttgaatttaa taacgcttta tcgcttgact
    31261 cttttaattt ttcatgaatt ttagaaaaaa caaaagtttg agaagaatct gtaatatagc
    31321 catcaattga aacgccaaca tcaactttta aaacatctgt ttctttaaga attacttgct
    31381 cttcggggtt ataagaattg tgtgcagcct cttggtttat agaaaggtta actggccaag
    31441 ctggttttgc gcctttctct tttatgtaat tttctatttc tgcccctatc tctaaaaaag
    31501 aaatgcctgg tttaactatc ttttttgcga atttttgtgc gccattattt ataatccctg
    31561 atttctttaa aatgtcgatt tctgttttgt tcattttttt tcttttttat ttgttaaaaa
    31621 aactaccgca ataaaagcaa caataaaccc aaaatatatt ggcatatctg gaattggaac
    31681 attcttttgt gcttgtttaa tcataaacca aatttggcca atgttattta ttggattatc
    31741 atcatcttct aatattgttg ctctaataag gtgttgagca gtagatgcat tcgaatctgg
    31801 aataataatg ttaacaaaaa tattattgga atcatttgct ccaatgctta ttgcatttcc
    31861 ctcaaaataa tttctatcaa aaatctggac tgcttgaggg gtaaatactt ctattcttaa
    31921 atttacatct tgggtagtac tagaggtgtt aacaacaata acattaatat caaaatttgt
    31981 atcttcaatt actgcttttg gtgcaattat ctcacctatg taaaaatcag caaaaattgt
    32041 gccagacatt agtattaaaa atgcaaatat aaatattttt ttcatatctt tattctccta
    32101 aatataatat agtaaatata aagacaataa ggtttataaa attagctaaa aaaagaaata
    32161 aagagaatta ggcgattttt gaatcgtaat tccctgcatt taaatccttt aaggcttctg
    32221 ctggagtctt gccatcaata ttaaccccaa gagatctaca ggttccaaaa acttctctaa
    32281 ttccggcttt aaaacttctt gcactcaatt gaccatactt tgcttttgtt atttttagaa
    32341 tcttatcaaa agtaatgttt ccggcaattt ctgttccggc tttccctgag gcctttttaa
    32401 tatttaattc tttttttatt aatgcgcttg ttaatggtgc accgacttca acagaatagc
    32461 tcttatctga attaatatga actttaactg gcacttgcat gccttctaat tcctttgtta
    32521 ctctgtttat ttctttaaca acttccattg tgttaacacc taatggccct aaagctggac
    32581 caagtggtgg acctgcacta gcttttcctg cgtctactaa aacttttatt gttggcatat
    32641 atttttcaca tcctttaatt taataattta atattttctg ctttcattgt tattggaact
    32701 ttgactgttg catcaaacaa ctcaataact acttcttctt ttgcaacatt tactcttgtt
    32761 acttttgctc ttgaaccttt aaaaggacct aagataatct ctacggtctg tcctggctta
    32821 atagaactca ttaatggtct ggattctaag attcccgcaa tctctttaat atttatattg
    32881 cctgtaacca tttgcccacc cttaatgtga gggacgttat gtattaacag ctctacggtt
    32941 ttttcctctt gggcttctat caatatgtag cctttaagat cagccatctc tactagagaa
    33001 tagactggaa gtccttcttc tttaattctg tggtgaagaa tatctataac aactctttct
    33061 tgaccaactg ttgttctaac tgtaaatatc atacttttcc gcctattata ttcattatta
    33121 ttcttactat aaaaccaata actccaataa caatcatacc aataccaacg atctttgcca
    33181 ttgcccagta ttctttcttt cctggttttt ttgcaatcaa tagaattctt tttgatgatt
    33241 caaaaaattc ttttatttta tttaccatct tttatcacat ttatacgctt tcaatattta
    33301 gttctaattt ttcttttttc tttcctatgt ttgttccaac aatgtcactt aagtaaggaa
    33361 tatctgcgcc tgcaattact gccataactt ttattgagtt tcttggcatg tcctcatcaa
    33421 ccatggcccc ccagataatg tgagcatctt gactaatctt tgaagctacc gtttcaacta
    33481 ttagctctgc ttctttaaga gtcatgtctt ggccacccaa aacatttact agagcttttc
    33541 tagcattacc aatctcaata tcaagtaatg gtgaggttag tgcatcttct actgcttcca
    33601 ttgctcttga atcgttatta ttcttgttta ttgagctttc tccaacaccg atcattgcta
    33661 gacccccatt tgaaagtatg gttcttagat cagcaaagtc aagattaatt attcctgctt
    33721 ttgttactag ttcaattatt cctttaactg attgtgttaa gattttatcc acttcttgaa
    33781 aagcaatctg taatggtaag tctggtgcaa tttctaaaat cttatcattt ggaataatta
    33841 ttaaagtatc aacttctttc tttaatcttt caagaccacc tagtgcattt tcaattcttt
    33901 gctttccttc aacagcaaat ggtaaggtta caacgcctat tattagtgcg cctttttctt
    33961 ttgcaatcct ggccacttct ggtattgcgc ctgttcctgt tcctccgcca agtccacaag
    34021 taacaaagac catgtctgcg tcttttaata cattctttat ttcgtctttg gattcctctg
    34081 ctgcagctct gccaacttct gggtttgaac cagcaccaag gcctcttgta gttttctttc
    34141 ctattagcat ttttttatct gctctaatct ttaataatgc ttgagcatca gtattcatgg
    34201 ctatcattat tgcgccttca atacctgtct gattcattct gtcaagacta ttaccgccag
    34261 aaccgccaac accaataaca tagatgtttg gaagagatgc ttttagaaat tcctctaatt
    34321 cctcatcatc acttctttga actttttcac tattctcgtc ttgtgcaact acgcggctgc
    34381 tttgcattcc tgacaaaatt cattccccct ggcttgttat cttatcttta aacataaaac
    34441 ataaatataa tataaaaaaa gatattatat ttatagacta gaaaccttta aatataaatc
    34501 tattttaggt tatatagaga cagaaagcta aaattaagga atttttatga tagataaaga
    34561 cattttaaga attggtaaag acattaaaga aataaaaata caaggcgcaa gtaagattga
    34621 gcttgctgca gttaatggta ttaaagaact tattaataaa agcaaatttg aaggaaacga
    34681 ctttttagga gtcgttgtag aaaatattaa tcatttgatt tctcaaagac caaatgaacc
    34741 aaaacttaga aactctcttt cttatatctg gcagatgtca aacaaatatt tcagtataaa
    34801 agacagaaat ttacttgttg atttgattga aaattatgag agaaatatta cgcaaaataa
    34861 tgaactgatt tcagaatatg cttctcagtt gattgttaat ggctcaacaa ttcttacaca
    34921 ttgccattct aacttagtag aaaacgcatt taagaaggcc tatgatagag gcacagactt
    34981 tgaagtaatc tgtactgaga ctagacctag gtttcaggga cgaattactg ctaaagccct
    35041 aagcgaatat ggaatcaaga ctacgcagat tgtggattct gcagtatcag ggtatcttaa
    35101 aaagattgat ttttttatga ctggtgctga tgttattttt caagacggtt ctattatgaa
    35161 caaagttgga acgcacacaa ttagtattgt tgctaagaat tttaagactc cgcacattgt
    35221 tctaacttcc actcattgtg tggagacaga tgtttttgtt tctttaaatg aatctataga
    35281 agaaagagat attgatgaga tttggaaagt tgaagacgga aggccaaaag atttaaagtt
    35341 aagaaatctt tcttttgata ttgttccaaa cgaattgatt gataaattca taacagaaga
    35401 aggtgttttt tctccagaaa ctcttgcact ctgggttagg aagaatttga aataaaaacc
    35461 caaattattt aaattaaacg gaaatttgat tctctataat taaattattg tttatgcaag
    35521 aatagtcatt tacctttata tctaatgcct taactcgttt tatagttaaa tctaaatatt
    35581 tcttatctaa atcaatacca atatatctgc gattatacaa tttagataca atgcctgttg
    35641 tcccagaacc attaaatggg tctaatacta gatctccttt gtcagttgtg gataaaacaa
    35701 ttcttttcaa taattctaat ggtttttggg tggggtgttt acccgatctt ttttccagtt
    35761 gaggtgttag aggtatccac caaacacttc tcatttgttt tcctgattca ctaatcttat
    35821 cttcccattt tttcataagt tcataattaa atttgtgttt agatcctttt tcttttcttg
    35881 cccaaatcaa agtttcgtgg ctatgagcaa aatatctaca acttaaattt ggcggggcat
    35941 taggtttaaa ccaagaaatt tcattaagta tttgataatc taatatttgt aatgcataac
    36001 caacttgata aatgttgtgc attgttccgg atatccaaat acttcctgta tcttttaaaa
    36061 ctcttctaca ctcagataac cagtcaaatg taaattcaaa atctttaaca accccgtttg
    36121 atttatccca atctgcttta tttactgaaa ccattttacc ggctttacaa gaaacgccgc
    36181 catttgataa aaagtacggg gggtctgcaa aaataagatc tactgaattg tctggcattt
    36241 cttttaatat tttaatacaa tctccttgat gaagattaaa atcaagagaa ttataatagt
    36301 tcataaagat atatacaatt agttatttat atatcttttg ggggtagttg tccagtggca
    36361 ttatggttta ggcaaaccta aaaattccaa gggtatttca ttaaagaaat aataacaatt
    36421 tactgatttt atataatcta aaacatcttt gtattctagt tttttgaacc aatcatttaa
    36481 cagataagtg tatttaactt tgatatttaa tggtttaaat aatttgttat attgtttatt
    36541 cttaaaatcg caagtctgta atttttcatc tacagaacca gccacttctt gaaatttaat
    36601 ttctataata aatagtgtgc gttctttcaa tacaaatata gcatcatcag gcaataactt
    36661 tttagaaata taattcaaat aatttatgcc acttggttct aaaaaatttt tataaatttt
    36721 gtgtttttga taaaaggtag caatcttttt accattaaag aaaatatcgt cgttctttat
    36781 gtcatatcct ttaatttgag taaataattg ctttaacgaa attctttctt caaatctaag
    36841 tccagtaatg gttttccctc caccgacgcc actttttatc atattatcac caattaataa
    36901 ttttttatta gtacttcctt aatttcattt ctttttgtgc catcacaatt tatcattctt
    36961 tttgctaaaa cttctttgat tttatatttt ttatattgtt ctttaataaa tggggttgga
    37021 ctattgcttt ctaaaacaaa acaccctttt ttatccaatt tatcaaatat attttttaat
    37081 ctcaattgat cggatctaac aaagtcatta cttgtataac ttgtaaaact attcccgttt
    37141 atttgatcat atggcggatc aaaatatata aattctttcg cctttgcaaa atctataacg
    37201 tcttcaaaag gcattacctt catttgtaca ttttgtaata atcttgatgc ctctaatata
    37261 ttttttttac taaatatttc tggattcttg tatttgccca taggcacatt aaattgattg
    37321 tctttgttta ctctatataa tccattaaaa caagttttat ttagataaat aaatcttgca
    37381 gctctttcaa tattatctaa tttattgtat tttgggtcac taagaacaat tcctaatctt
    37441 tttgttgttg gagtttcaag tcctctaatg gtataataaa attcttctga atgcttagac
    37501 ttatatttct ctaacatttt aattaattcg tctggctctg ttttaattat taaaaacgaa
    37561 tttattaact cttcattaat atcagaaatt aaaactcttt ttgggggaaa gttttctaaa
    37621 atatagaata atagtgcgcc gccaccaata aagggttcat aatagccttt tatttctttt
    37681 ggcatagtag aaataatctc ttttaatagc tggttctttc ctccagccca tttaacaaaa
    37741 ggttttgcca tatgttgtct atttttatta tataacttat aattgaatat atttataaaa
    37801 tctaacgtgt tattcttttg tgaaaatgag ataatgccca caaatttagg aattatataa
    37861 aactctaatc tgttaattta aaattggtta ttgtaatcgg ttataactac ggaggataat
    37921 taaaaaaatt aaataaggtg cttatatttc ttcatagcct ctttttcaga aagatatttc
    37981 aggtttttct ttttcatttc taattgttcc ttttttagtt tctttgcaac aagcaataat
    38041 tctttatctt tgcttaccat tattatcccc ttttatcttt ttagaaaaat tctccaagac
    38101 ctttttgctt ggtgtttgtg actagctcgt cttttgagat tccaagctct tcaaggatcg
    38161 gaagtattgc tggaactaac tgattgttaa tataataatc tacatcatag tctttgtctt
    38221 taacaaactc tgcaatctgt gacttgtctg aaatgctttt gcctttctcg gtgattataa
    38281 attcaacaag atctccaacc ttaaatttct ttcctgcttt ttttgcgttc ttgactgctg
    38341 acattgcagg gtttaaggtc ttatagcttt caagagattt atgcacaaag gtttgaatga
    38401 ttagcttgtt atttggaatt tctttatttt ttaaatcctc aattataaac tgaagatatt
    38461 ttaaagcctt aacgttatct tgctctttta gtacaatctt taaaatttct ttttgtgttt
    38521 ctttaacaag aggacaccaa tcatgcctta catattcaaa gcccttaatc ttaaggtttc
    38581 cattgtaatc aagaagagca tacttcttct ttgctgctct ttctttgtct cttgcgtgca
    38641 caaaaagacc agacatataa atgttttcta gttctaaatt cattgctttt ggaagattgt
    38701 tatttatttc tctaagatct tcttttagtt tatcaatact tctaaactca ataaaacaag
    38761 aatctgtgtc gccatagatt actagatagc ctttgctttc aagaagccgt atcatgtctt
    38821 gtatctttga tcttacaaga taatacatta tctctagact agacctagaa taccagcgag
    38881 caccagaaaa gcccatataa ccataggttg aagccaagaa tatctttaaa gcatactgct
    38941 tagcaaacat gtttctgtat tcttgtgtgt ctttttctaa aagctttgcg ctttctttat
    39001 agcctgctct taatgtcaat aattcttcaa ggacttctgg aacaaatcct ttcttgtttt
    39061 tacaaatata gtgacctgta atattttcta aactggattt gtcacaacat gagcaatcaa
    39121 cagtctctgg agaaatatta taagatatca taatagagat atgatagctt ctaaaatcca
    39181 gaactgcgat attcttgtgt aacccaggaa ttggagactt aacaaatgct ccactaaagg
    39241 tgtatttctg cctttcagaa atctcttctg ctcttggctt gtttggaata atcttgtttt
    39301 tcttaaaata atatttaaag aacaatcttt caaccattgc tcctgctgaa gaaaaaacaa
    39361 tatcattaac tggaaagtta ataaaatcag aaatctcaaa agctattggc atatagttgt
    39421 ttgctagttc tattgttgca atggtgtcgt ctagattata atcaataatc ttataataat
    39481 cctttgaaag ataaagctct ctcatctctt ttggtgggag agtaagcttt gggtttcctg
    39541 ttagttttga atatagagaa tttaaatcta gctttggata attaaatacc tgtagatatc
    39601 ttagtaatct cattaaggca tagacatcaa tatgaattgt tccttgaaga ttgacttttc
    39661 gagtttgtga tttagaaaag attaagcggc tgtttgaaaa gcttaaagac ttgccagttt
    39721 ttgccttata ggttttttca agatattcaa aatcaaagat tccgttgtat gtgtaaacta
    39781 tatctatatt tttagaagca ataaagtttg taaactcctc aatcatcttt atttcatctg
    39841 taaaaagatg aaggtctaga aactgatagt gcttttgtag ttcttctttt gttttttcta
    39901 aattgtctgt tttatctatt agaaaaaata tggctttttg tttgttattg tctgtgtaag
    39961 caattgaaat tattttatca tttccgggtt ttggaaaaga caagctttct ggaactaaca
    40021 cttctatatc aaaagaacaa atattaagtt tagaaagatc tgtattttct tttggtatta
    40081 tttctgttat tttattttct gtgttaagag tgtattctac ttctttaaga taacttaaat
    40141 tcttatctaa tagaaatcta taaacaaaag gaatatcgta ctcgtaaagc ttgtatttct
    40201 tgtttgggaa aaaatctgtt tcaaaaaaag cagttcttgc ttttactaag tcttctatat
    40261 tttcaaaaat cagtttatag acgttttctt cttttagaat gattcctaaa acgttttctt
    40321 tagattttag attttcgatt tcttctgaat taagtttttg gtttaaagaa agataaaagt
    40381 acggctgata gtcaactgga taagtctgtt ttatctggtc ttttttaata gtaacaaaaa
    40441 gttggctttc tttatagtaa gcatcaataa aaattccagt gttttctttt aaattttgat
    40501 tattatcttg ctttggcgct tttttaaata tattaaacat aattaaaaac tatttatata
    40561 aaaataagaa aaaattatat aaggcttatt aagaaaatta aagccattaa tactaaagtt
    40621 aatgctatct tgtagactgg ttttttaact tcaaatccta tttctgtaaa ctcgtaaagc
    40681 attatcaacc agtaaacaat cataaatatt gttaatagaa atactaaaat tgcaccaatc
    40741 atgtttgcaa gagttaattc tggaaatagc gttgcactat ttattaaact tggattaact
    40801 gcaagcattt gaagagaaga aatgattgaa gggaataatg taaaaacaag taatgcacaa
    40861 agtatagtat agattattga ggttgctcta aatgctgcga ttgcagaaag cactttttca
    40921 tatggttttt ttggaaggtt tttttgtcct cttataaaat acattattag atagattgca
    40981 agacctaaga ctagccatgt tagaataact ccagtaagca actgattaag tatgtatatt
    41041 cctaaatctt gacctttaaa tagcaaataa atcaaaatat taattattat tgcaaaaaga
    41101 acaataaata tgctttcttt tataccaatc ttaaaaaaga aataatcaat tatagctttt
    41161 ggtcctcttt taggcttata ggcctctggt aagtttgttt cagtttttct agttgctggc
    41221 atttttttca ccaaatttaa ataaataacc taaatatatt tagataaaag aatatataaa
    41281 tgttatgaaa aagaaaaaag agagattaac tctcttgttt gaaattatct ctgcattaca
    41341 gatattgtat attcgccaga aaagatttcg ctattttcac atccttcttt tactgccatt
    41401 gcttgtagag tcatatctgc gccatagaat tctatttcgg atgaatatgg tgtgctttct
    41461 attgttggtg ttgaaccatc tgttgtataa tagattgttg cgccgtctgt tgaagtacta
    41521 agtgaaatca tttcatagga ataagttcct ggaacaggat ctgctgttgg tgttgcaact
    41581 tttcttactg gaacatttgt atttggtatg ttttctaaag ttataaactg gttatcagaa
    41641 gttattagaa tcactgttat tgttgaagca gtaatctcaa gatctgcaaa accttctaaa
    41701 tttattacat ttgaaccttc ttctaaagga actgggtctt ctaaagttac ttctattgtt
    41761 tccatattgt ctgctataac tttatacccc acaatcatta tttcttgatc ttctatttct
    41821 gctggtggac tatagtttaa agagacttct cctgcatcta ctgattctgg gtagacataa
    41881 ttatctggat ttgcctgaga atttgtaacc gtttttgtat tatctaaact acttgaagac
    41941 gtttctctac tccaagttaa aagaatagta cctagaatta ctgcgacaac aactaccaaa
    42001 attactgtta taattggagc aatccctttt agtttgattt ttaattttga tgacatatta
    42061 tgatgacata ttattattat tatatattaa aaacgtttct tttttagttt taaaagcatt
    42121 taaatatttt tagaatacta tattctatta atggctagaa aaaaacaagt gttctctaaa
    42181 cctttcaatc tggtttatga aaataaatat cctgatttgt tagtgggtcc tggattctat
    42241 gaggaacaaa atattgcagg ggattctgat ccaaaaacct ggattaagaa aaaaccagaa
    42301 gagattgaaa gagaaagagc taaacaaatc tttactttta aaagttttag aaaaacagac
    42361 gctaataggt ttgataaaaa ccttacagag atacaacttc tttcgcaatc agttgatacc
    42421 actgaaattg aagttggatt agaaactaaa gcaaaaaaga ttaatgaaaa ctatagtggg
    42481 attaataatt tttcttatgg tcttgaagat cttaaaatta ttgataatat aaagattcca
    42541 caaagtattg ataaagttgt tagcgatggc gaccttaaag ctaaagaagg aatattaaga
    42601 ttaaatgaga agttagaaga cgtttataag attgaacaga tactgtctat gggacttctt
    42661 ggagtaacta aaaacagagt acttgttcca accaggtggg caataacttc aactgatgat
    42721 attatttcta aagatttgat tgagaatact cttctagatt actcaacaat tgacaaatac
    42781 gaagtatttt cttttatgtt ctatcaaaat atcttttatg tgctacttgt tccaagactt
    42841 tggggctttg agcagattga gattcaaggt gagaatgttt gtgaggatta tgagattaat
    42901 ggaccaagaa agaagtatgc aagcagcgtg actggcggat actatgcttc tagaatcgaa
    42961 gttgcaagat atctttcaaa aagaaaaaga caagcttctg ttattgtttt tagagacatc
    43021 tctcctgagt atcgttcaaa aggagtttgg gtgattagag aaactgttaa agaagcatta
    43081 acaaaagaac cttatgtttt tgaaactctt gaacaagcaa cgggttttat taatcaacat
    43141 atcagaaacg gaataagtta ttggctaaac cattcagaac tctataaaga aataaaaacg
    43201 caaaagaaaa ttactgattt ttttaaatta taaaaataga ataaaaccca atatacaaaa
    43261 tatcacttca acaagaagta tgcttgtggt tacttgcttt tctgtaaatc ttcttttaaa
    43321 gagtgttgga agaaacatca ttatgtgtgt ccaagaataa atgcgtttgt atggcatctt
    43381 tagcgttccg tctttttgaa gattgccaag acttctaacc tttagctttc ctcgtagttt
    43441 tagaattatc tctatgcccc aaggtatgaa aataaataat gcaaagcttt caatattgcc
    43501 aataatagca atacttgcaa aggttgctcc gaaaagatat gtcaaagaat ctcctggaag
    43561 cattttggca ggataccaat tatttagaag atatggtgct aatgcaaata aggccataaa
    43621 cgaaataact gctgcttcag ttcttccgtt aattattgaa aagatgccaa gagaaatcat
    43681 aacaattgcc attaacccgc cttctaaacc attggcacca gcaagcatat ttgtagaatt
    43741 tgttataacc acaaataata ttggaacaat taaaagtgca tacaacactc caaaatttat
    43801 tgtaccgatt aacggaattg ccattgtaga atctccggcc attatggcaa tcaacggaat
    43861 tgcagcaagc accgttaaaa gaggctttag ccattgtgga aggccaactc ttatctctag
    43921 atctccatat atattcttta ctggttcttt actaatattt atatcatcaa agaagccaat
    43981 taatgtcgca atcaaaattg aaagagttgt tgcaataatt aaagttatat ttgtctgaaa
    44041 ataaaaagca gaaagcaata caaaaaacat aatgccaata agaaaaccaa aaaatacagg
    44101 aatgccccct gattgaggca aagttggttt ttctttcttt tgttgatcaa cagcaattat
    44161 gcctcttgtc acaaagaatt ttttaaattg cgttattgca aaaaaagaag ctaaaaatga
    44221 aactaaacaa cttaatatta gtattatata gttcatttaa tttgcctagt ttaatttgtc
    44281 tttattaaca ctataaactc ttacgtctcc agatgaatag gaaagctcaa taaatggctc
    44341 aactgttgga tcattaaacc aaactcttgc aaatattgtt ttgtttattg ctgcatttat
    44401 taaataaatt ctggaattgt ctttttcttt ataaagatac actggcattc cttgcattat
    44461 ttggtcaggc gcggtgtgat attctgtttt tatttttgcc atctctgctt ctggcaatac
    44521 attcccttga cagttatagc ttactgcacc agatattggt gtaactactt ttgaacagcc
    44581 aagccctaga ccataatatt ttgtgactct tggatcacta aagtttgtgg tccaataacc
    44641 atatatcgca aaagaagaca ttctagttag atatgatgat tctaccataa aataatctgc
    44701 atcatatttc tttagcatac ctttagaaaa attaagatct tcagaaatca aaaacatacc
    44761 aaagtcgctt gttgcttcag cactcgcatt tgtattatct gtgcttactt ttctttctgt
    44821 aaaatatgca atccagtgtc ctgaatccca ccagttaaat atttttgcat cagctgaaga
    44881 attatccttt acccaaaaca ttgcattctg ccaatccttt gcttcgtcaa ttgggggaac
    44941 cttactcatt acaaaaaaac ttcctgcggc aattgcgcct attaaaaaga ttgcagaaat
    45001 tattgttagc gttcttttgt aagctagagc tctgtgttgt agcttttctg ttgctaggaa
    45061 aaacaagaac ccaaagcccg cggcaattgg taaccctaga gtgtaagtaa attttaattt
    45121 tgtccaagcc ataaatccag taattaatat ccaaaagaaa ataattaaaa caaaataatc
    45181 aatattatcg tgtttataat cttcattttc tttttctttg tttttgtatt tgccaaagat
    45241 aagatataat ggaattatta ataatgctaa aaatggaata acaactaaaa gactatattt
    45301 ctctaagaaa aactgcttac cagtgttttc ttcaccaacc gttgctccaa ttacatcttc
    45361 tgctgctaca ttttctttgt ttattctatc tatgttatca gtagatattg gcaaataatt
    45421 tgtaacatac tcagatgttc tttttatcca gccagttcca ctataggccg tagtaattat
    45481 tgtaaacaat aaaaaacaag ttaagaatac ttttgaaaat gctagtattt cttttttgtt
    45541 tatttttttt ataagcatat aaacaagcat tgtcccgaaa taagctaata gcaccaatgg
    45601 aattaacaaa aacatatccc aggttattgc cattaaaaag aagaaaaatg cacctacaat
    45661 agaagatatt agccagttct ttaatttatg tctattctgt attgccataa ctaagaatat
    45721 aaaacctaat actagccaca aaaatcctaa agaatcttct tcaaagaatc ctcccattgt
    45781 tctgtagaca aacgcgggga cggtggctgc cattactccc attgcaacgc ctgttctggt
    45841 gttatacatt ttcgtaccta agaaaaacat agcaacagaa attaatgccc caaagattgc
    45901 aggaagccat ctagcatatt ccatcaatag ccatttatca taggctgctc caaaggttgt
    45961 tattgtataa actgctgctg taaaaaacca taagaagttt ggccaaatcc cgtagttcgt
    46021 tccaccttgc tgatagaacc ccaaaggatc gttagttggg agatctcctt gaacgacata
    46081 gcctgttgct ctaagatgcc agtatggatc atattcgaac atatagtcat atcttaaaat
    46141 gtgcgccctt attgcaaggc ctaaaacaaa aatcaggact gctattaaaa ttattagcca
    46201 gtgtttagaa aaaaaatgct taatcttctt cattgaaagt caactcttat taaaatttgc
    46261 ttgaatcact taaataataa gagccattat gtttttaaaa gaaatatatt ttagaaaatc
    46321 aagttataaa aaggaaaata aaaaattaaa acaaaataag aataatttaa gttagcaact
    46381 aacttgctgc tccaagaaag aacttagtgc aagacaatat cggagataaa ctttacttct
    46441 gggttcgaaa agggtccagg tgtttctcta tccctatggt cgctaacaaa taatatatga
    46501 ttgataaggt ttttaaatag ttgggtttat ttctttttct ttgaagtctt ttcttctgta
    46561 tttacgtctt tttgaccagc taaaaccttt tttaatttgt ttagaccttt aacaatatct
    46621 tcttctgatt ttgcaccagc acaaattaat ttcccgttct tgaaaagtaa catcgtaagc
    46681 tttggattaa aaagtctaac aattgcgcca ggaaattgct ctggttcgta ttcaatatta
    46741 tcaagagtta ctgccgcatt aaaaagatca atctgcttgt taagatttga tgttgcaaca
    46801 agattaacaa tcttataact tactctttct tttaaagtta cgcctttttg tacttcgcct
    46861 ataatctttg ttgctttctt aattgctgtt tttatgtctt tttcacaaga cgcaccagag
    46921 attattatgt ttccattctt aaatagaagt aaagaaactc ttggttcgtc catctttaat
    46981 attgcgccag gaaactgctc tggttcgtat tcaacttctg gtattgctgt tgcaagatta
    47041 taaagatcta aaccagcccc aagagagctt gaagcaacta aattaacaat tttatattcc
    47101 atttttttct cctttaaaat ttttacaaaa agcaaatgtt tatatatata atttatatac
    47161 ccaagcattt ataaactaac tttttggttt ttttaattaa ccgtttgata gtgtttatgt
    47221 tagatttcct gatttttatt attatttttg tttggctttt attttgcaaa tagtgcctta
    47281 taaaagcttt ttgtctcaat aattatatat aaaaataaaa aaataaaaaa ggaatgagaa
    47341 aaaaatggca aacaaaaaag caaaaggaaa gagatcttta acgcgaagaa gatataagag
    47401 aaatgtaaga gaaaaaacaa cagttaatca actgattaaa ccaattaagg ttgaagacgt
    47461 tgttcaagtt aacgcaaata gtgcagttca ttcaggtatt ccacacagaa gagttgatgg
    47521 cataactggt aaagtcattg gctttcaagg aaactgccca atagttcagt taaaagacgg
    47581 aaacaaaaca aaaaagataa ttacacacag agtacatctt aaaattatca agcaaatagc
    47641 aaagggtgaa aagaaatgat aaataaccca aattatgttg atgatttaag ccttgcagaa
    47701 gttaagaaaa tcttaactga aaaaggaaaa gacaaagaac ttaattatga acaaaagctt
    47761 gcacttgaac atgcaaagca atttgcaaag ttgacaccat taaaagcaga gaaacttaaa
    47821 aaagaactta tggagatgga actaccaaac gaagtagcaa caaagattgt tgatatcctt
    47881 cctaatgcta tagagttaga tttgatagca gagaaaaaca agacaataac tgatgacaac
    47941 aaagaaaaga tattaacact tgtaaacaag tataaaaagg atgaataaat aagttatgat
    48001 agaaattaaa aaagaagcaa ttgttcttga cagagcaaca aaagaaatac aattcaaaca
    48061 gacgccaatc attcagtgta ttggtgtgtc taacttttct ttattagaat tgtatagcga
    48121 tgaaactcct aaactacagg agacaataag cacagaagac aagcccataa aaagaataac
    48181 ctatgacaaa ttaacaacca ttgctaaaaa agaactagaa aaggccattg aaaccattgt
    48241 aaataataat caagacagat ttgttacttt ttttaataat gctagaccaa taaatgctag
    48301 aagacatcag cttgatttac ttccaatgat tggaaagaaa cacagaatgg cattaatgga
    48361 atacttagaa aagaatggac caattaaaac acttgaagat cttaaaaaaa ttgagttatt
    48421 ccccgatcca attaaagtaa ttgttaatag agtaattaca gaaattgaga ataacgaaga
    48481 cgagaagttc tttttgttta ctataccaat tattaaaaaa ttctaaattc tttaaacatg
    48541 ataaaagacc tatactctca aatgttaaaa tataacttta agcctagcca tagcttagat
    48601 caaaacttta ttgttgatga taaggttatt gaaaaggttg tagaacttct tgatatcaag
    48661 aagacagatg taattctaga ggttggccca ggaactggct ttctgaccaa acatcttctt
    48721 gaaaaagcta aacaagtaat tgctatagaa aaagacaaaa aaatgtttga gatcttaaag
    48781 caagaattta aagaaaaact tgaaagcaaa aaactgcttt tggttaatga agatattcta
    48841 aaattgaatg tttttgatta taaatttact aagattgcaa cttttttacc ttattctatc
    48901 tctttacaat ttacagaaaa aatattgaat tgtaattgct tgattgttga gattgtacaa
    48961 agagaatttg cagaaaagct atcttcaatt cctggattta ttaattataa tgcaatttct
    49021 gttttaactc aaagttatta tgaagtagaa ttaaagaaaa acgtttcaaa gaaatgtttc
    49081 ttcccagttc ctgcagttga ctcttctatt gttgttttaa agccaaggaa aaaagaattt
    49141 gataaaagat ataatgattt tgtaaaaggt ctttttagat atccaaacaa agacgttagt
    49201 aatgctctta atctcttctc aagagatgtt aaagatagaa gcatttctga aaaaaacaaa
    49261 aatttagaaa acaaaatacg cgataaaaaa ataagacaaa tagagattac agaacttgaa
    49321 aaaatctatt ctattattta ctgattaact tcttcaagta ttatcttttc tattttgatt
    49381 gtgtctgtca ttgtggattt
//
LOCUS       DAOOON010000003        48828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_788_length_48828_cov_18.422283, whole genome shotgun sequence.
ACCESSION   DAOOON010000003 DAOOON010000000
VERSION     DAOOON010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 48828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48828
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_788_length_48828_cov_18.422283"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1365)
                     /locus_tag="PLK55_00785"
     CDS             complement(106..1365)
                     /locus_tag="PLK55_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ35505.1"
                     /translation="MNKEYLTQIILDQNKRLEKLSEGLIRQKLSEITSFINHKINIVI
                     TGHRRAGKSTFLLQWMSKYYKDKFYYFDFSDERILDFKTKDFQLLHEVLLEQFGEKKI
                     FFFDEIQGKPNWDKFVNRLYTEGHRFFITGSNAELLSKEISTYLTGRHFDILIYPFSF
                     EEYLRYKDFKKNSKTTEGKTEINKHLKEYIKKGGFPEVVVYDNSEILSSIYNDVINKD
                     IINRYKIIDETTFKKLAHFLITNFSKEFSYTSLKNNFNMGSTHTAKKYVSYLVNAYVL
                     FELNKYDPSLKNQERAAKKIYCIDTGLINQMAFKFSENLGKLYENIVFLELLRRGKKI
                     YFYKLDNGWEVDFLVKDKDKIVSAIQVCYDLADTKTKERELKSLLYTCDKFGLKDGTI
                     ITNDLEKEEKINNKIIRYIPLWKWLIE"
     gene            complement(1467..4319)
                     /gene="ileS"
                     /locus_tag="PLK55_00790"
     CDS             complement(1467..4319)
                     /gene="ileS"
                     /locus_tag="PLK55_00790"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOZ35506.1"
                     /translation="MAEQFSNKTELEIKNYWKVNGIPEKVRAKKGTKNYYFIDGPPYA
                     SGSLHLGTAMNRILKDLIIRYRRMSGYTVLDTPGFDTHGVPIEAKVQKKYALKSKEDI
                     EKFGIYNFTKECYKFATEHIDDMSKEIYNLGQWMDFKNPYRTLDKSYMETGWWVFKKA
                     TDKGLLYHDKYPVHICPSCGTAVSFNEIEHKDLTDTSVYVRLKSLDMPSTYYVIWTTT
                     PWTLPANLGIMVHPNFTYVELIYNNEKYIVAEELVEKIIRELGWKDYDLGKKYKGQEL
                     AGKKYEPILGEWLEKLDELKKDCYKVALSPRFVHLEEGTGLVHCAPGHGREDYTVGKE
                     NNFPAYCPVTVEGVYDSSMKVFKGEKVKALDPKIIEYLEKKQAILGKRSITHAYPTCW
                     RCVSPLLQVALPQWFLRIEPMKERLREINQKEVTWYPDWAKERFDNWLDSLSDWPISR
                     ARYWGTPIPIWQCDKCKEIEVFGSLEELKIKASAVDVNMDLHKPGIDNITYACKCGGT
                     KKRIPEIFDVWFDSGIASFASLGYPKKQETFNQFWPPDINLEGSDQIRGWWNSQMLVS
                     AICFDKAPYQFVALHGMVLAVDKKKLSKSLGNDRPLAQRFSETSIDYYRYYFSREYNG
                     MDVIIDEKKFSDIKRVFNLLENIYSLMALMDDKQKFNPSIDNDKLEIEDKWILSKYNS
                     LVESYHKNYSGCNFSKATLDLEKFILEDFSRTYIKLIKKRETKNNVLSHIFSGLLLHL
                     SPAAPHLTEYLFLKFKDRKETIHLEELPVANEKLINKELEDNFEIALAIVQEALAARE
                     RTKKRLRWVLPRLVVVSPDAEKLTGVKEIVANMANIKEVLLQTTIPKGNFEEGKVSDN
                     LSIYLDNDLPEGLEDEWELSELTRAIQAERKKNNLTPSQVVTLHISCSDEAFIKIKKQ
                     VIEQGTATRISYQVFEPKTPSKQTLIKREVYFSFAL"
     gene            complement(4391..5842)
                     /locus_tag="PLK55_00795"
     CDS             complement(4391..5842)
                     /locus_tag="PLK55_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35507.1"
                     /translation="MILKDTKKIFLWIFGLIFSGIVYFILKSNYIFDTSKNPYLPLAI
                     SLVIFLLFFGVGIFVSQISNIFYLVLSKSRDLKEVQKIAKDEQKEKQLKLIIKNIRRI
                     VNYILIKNYSKESVKALSDYIGLDSEATERLVKIIKETTKIRFIYWIIGILLSSLNMI
                     LIFSLDIFVFLRSPMIVPIIVSLVIYMLFFIEGFLILRLPQHVYLDVLNITERSKEER
                     QYALEKNKEELSKKESIQKRSIQNIRNTIKYLIEIGANRDWILDLLVSNGFSKGVAQD
                     IMFEIIKEGLKDKELYKAEIKEIKNMKKTKELFVKKMSEDFNNLREIYQEVSYLKNFV
                     STLEDKEIKVEKFREKKVSYKTFDLSEIKQTEDMSASINEIKRRRDYANIPVAGASKE
                     SRRLTAQEAKESLPKNTSTNQQINFLYVLIRPYANKYTREELQSMMLSLGYSVAMIDD
                     VLILLEQNGIDFKQDEKGIIPFVDNLLDKATKK"
     gene            complement(5907..5981)
                     /locus_tag="PLK55_00800"
     tRNA            complement(5907..5981)
                     /locus_tag="PLK55_00800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5944..5946),aa:Ile,seq:tat)
     gene            complement(6053..6397)
                     /locus_tag="PLK55_00805"
     CDS             complement(6053..6397)
                     /locus_tag="PLK55_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35508.1"
                     /translation="MILEKIRDIKHFQKKVDLIAKVIEKTNIREVTSKLDETKHTVCE
                     VLIGDDTGTIYLTVWDEAISKIEIGKTYIFRNLFSSEFQKSMRLNIGRFGEFEETDQQ
                     IDVDLENFRSQV"
     gene            complement(6494..8119)
                     /gene="thsA"
                     /locus_tag="PLK55_00810"
     CDS             complement(6494..8119)
                     /gene="thsA"
                     /locus_tag="PLK55_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOZ35509.1"
                     /translation="MGNESQNTMFLPEGSKRERGKDAQRMNIMVAKAVSNAVKSTLGP
                     RGMDKMLVDEMGNVTISNDGATILKEMAIEHPIGKMLVELAKNQDDEVGDGTTSAVVI
                     AGGLVAKAENLLDENIHPSIIIKGYKMASEKAVDYFNEISEKVTINDKAELLDIAKTS
                     MTGKASEYSTNLGNIVLEGILKISKSNKEIERDQIKIEQKIGGSLSETKLIDGIVLDK
                     EIVHSEMPRNIKNAKILLISSALEVKEPETEAKIQITSPDQLQAFIDQEESYLKAMVD
                     RIQATGASLVLCQKGIDDLAQHYLAKCGITAIRRVRQTDIDRLAKATGGKIVSRVKDV
                     TIKDLGTAEHVYSKKVAGDDMLFIEGCPNPGYVTILLRGTSEQVLAETERTLTDAIGA
                     VISSIKTGKFVAAGGSSEIEVALRLKNFAKTVGGREQLAIEAFAEVLEIIPKTLAESA
                     GLDAIDTIVSLRSKHQDSSNKALGIDVVNGVLADMKKKKVIEPIAVKTQAVTSAAEVA
                     EMILRIDDIIAGSSNKPRGPPMGMPGGMDMGDM"
     gene            complement(8139..8546)
                     /locus_tag="PLK55_00815"
     CDS             complement(8139..8546)
                     /locus_tag="PLK55_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35510.1"
                     /translation="MSENDLENQVIQPVEEEIIDAVEDEGIELDDNKLPFPNATVVRL
                     MRKNLDKHKQIKGIVKKEMNLFLGRIVETICKKMNSYEYTSVDYGMFKQSVETFENIQ
                     SIELEKIRIIKNLEKIKAECDILIDDVERKFHQ"
     gene            complement(8680..8841)
                     /locus_tag="PLK55_00820"
     CDS             complement(8680..8841)
                     /locus_tag="PLK55_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35511.1"
                     /translation="MYVINNDLEGQVFKMDDGEEKEDEDFEDFDEEDESEEEDEDFDE
                     EDDDFEDEE"
     gene            complement(8879..9049)
                     /locus_tag="PLK55_00825"
     CDS             complement(8879..9049)
                     /locus_tag="PLK55_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35512.1"
                     /translation="MAAKDEEKDYDLDDFDYDGDESIKEEKEDIETEYTPEDFEDEDE
                     EEFDSDHDFSDD"
     gene            9156..10103
                     /locus_tag="PLK55_00830"
     CDS             9156..10103
                     /locus_tag="PLK55_00830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOZ35513.1"
                     /translation="MYFLTKISMGEVYLPKEFIEKYKWLLGDQEALVFFDSCKTRLTK
                     SLIINTLKTKDASFLKEQGIELEKVKYIDNAYFVKSLVSGLGNTIEQNIGLFQMQEIS
                     SMLPAFVLEPKETDIVLDMTAAPGNKTGILAQIMNNKGMIVANDVDKTRIKTLKFTLK
                     RLGVKNTIITCRDGTKINFNFKFDKILLDAPCSCEGMIRKKPDSLKTWSHREVNSKSE
                     LQKKLIDNAFSLLKPNGILVYSTCTLSPEENEEVIDFILKKNKNAKIQEIRITGLNYS
                     VGLTKYKHKNFDEELKKAIRIWPHKSEIEGFFICKIRKE"
     gene            10106..10591
                     /locus_tag="PLK55_00835"
     CDS             10106..10591
                     /locus_tag="PLK55_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35514.1"
                     /translation="MTKILDEKTRKKLINYLNSRFGIDGSFFEDFLFIISGKSVSIIY
                     KTRETQKIIENQLRQNFTISFGIEIFSNYKDYRPSSLGFSSFEKKQIKQNFVELTRIQ
                     ANTYFSGKDIAIEDLKNKNLLSSGFVACISENHIIGTASFDKEKQIIIPNLSFVNENT
                     K"
     gene            complement(10592..11461)
                     /locus_tag="PLK55_00840"
     CDS             complement(10592..11461)
                     /locus_tag="PLK55_00840"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOZ35515.1"
                     /translation="MIKDLEQLLNKGYYYRAQKKKEIAILIIHGFAASPIEQKPLAEF
                     LKDDYDIYAPILPGHKTNITDFSKQKYTTWLSFSEKIYEELLKEYSKVVLLGFSMGGT
                     ICLYLSIKKRPYKLITMSAPINFIDPSFGKLILADWKRTNIKLSTIISEINEISKKQS
                     QTKQSTAEKIRLIINNIYSKRRKDFIKLNNELLEYEDTYKEISTNAIHQIFQLVGLVK
                     KQISKVNADILIIHSKKDYLIPVSNSKEIMLSVSSNNVERFLLEESGHQVMLDTEYET
                     VFKKVKEFLSREI"
     gene            complement(11490..11564)
                     /locus_tag="PLK55_00845"
     tRNA            complement(11490..11564)
                     /locus_tag="PLK55_00845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11527..11529),aa:Ile,seq:gat)
     gene            complement(11569..11641)
                     /locus_tag="PLK55_00850"
     tRNA            complement(11569..11641)
                     /locus_tag="PLK55_00850"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11605..11607),aa:Gln,seq:ttg)
     gene            complement(11719..12906)
                     /locus_tag="PLK55_00855"
     CDS             complement(11719..12906)
                     /locus_tag="PLK55_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOZ35516.1"
                     /translation="MIKGLETRVVIVGGGPAGLYCGYLIKRFNPNILVTIIENDKEIG
                     IPKACTGHISIFGLKKLKLTPFLDIDKLVVNRIRGANIYGPNKAELQFRTKRFQTVVI
                     DRIIFDKEIEKLARNVGVEILLGHQVVSVQEGSVAVNIIDDNRLIYLDYTYLIGADGP
                     NSIVRSAFTEKPITAEFVSTYQIIANGDFTKDMVNLYFGDFSKNFFAWIVPESYTIAR
                     IGIGVPLGQNPKQALDDFIAKRNIKLTNIKFDCSGQIPIAKPINSYFEKNKLLIGDAA
                     CFVKATSGGGIIFGLTSAEYAAKAITERLKNFKKVENYNKHLKSLIRELNIHYRLYKF
                     KNQKTNLEFDNLLLKLKDSGIESFLNNNGNIDYPSSFIPKLVLYPKFLLFYKELIDII
                     KSK"
     gene            complement(12910..16512)
                     /locus_tag="PLK55_00860"
     CDS             complement(12910..16512)
                     /locus_tag="PLK55_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35517.1"
                     /translation="MFLRKVILVLLILILVNSSIFAAVSEITINSGYNYVNIGKSVFF
                     TVTADSYPALLHISSNIDDGIDFNVQNSDGSAVVSFSAQSASITEGIPTIPVGDSDIV
                     TISAVGGGASATFTVDAVEPTNSAVISVNYNGGIPNYLNFYSGAITVDYSNGTISDSE
                     TYIDKHIVYLVPVSSLNNYSSAYTTEYVDTNAIRTKDINSLAVPIISDGNYYIVIDAN
                     DIAGNYSDSDAVKNTKVNVYFDNTEATISDVIIKDSSVGNVSTGTVYTDKNVFDLAVI
                     LSDASGIANESTMTVKLPNGGSRTDSSYSSGFLISEEEISAALAGHQVITSERYNVEI
                     SATDNVGNVYQDDFNIIVDLTPPSIPQKNELSIEDYDKNVTITWESGASSDGAGSGLK
                     EYRVYKDDTTFTEISNQTLVCTTSTTTYTCKDADDKDIGDTIYYGVVGVDNVGNISDA
                     NTRSISTGPDNCTVKINDGDDFTNNPVVQLEIEFSDDVNAIGISCNNGSSTAIAADDN
                     ISFNLTTGTGCSTTNEEKTIYVKVISEDDPDRFSICSSDIIYDNTAPTVPTNLAATIQ
                     TDGSAKLTWTESTESVEPDTVKYRIYYSTSSNLTNTAEYKESTTNNYTFMSNQDINLY
                     FKVSALDLRNNESALSSSVLANIKKIGPRFIIEVNPSNDVNGEIYVNSGAKTIEFLSD
                     EALSENPQVSIKKANSSTYENILSTYDSTTKKGSATTAFVSSGINTIKITGENTKNET
                     AYSELEIIMDSTTPDFNVTHELVDSVTYIIGLYNLPEDIFKVEYSVDDLNVIYSRQQV
                     TDYNFSEYDLSETPDGNHTMYIHVYDKALNKKTKTFNYVVDLIDEDKVTCDLLRDKIA
                     NKLSTLEEEIDLFKAIKIIDSKLEQTINDKKTIIFTKRSEGNDKYSDQNYLSAKADYQ
                     FSLNEIQAIDDLLPEIEIIKSTTLSVIYDANVPVSLEEILDQNILVKTKALYDSNTIK
                     VDRNFDALKIGTQNFFSVKLIIKNIGTISKEITIVENIPKSFASNVSNLIFNKEVEIL
                     VKDPIIKYNTAISPNGSEELIYTSLIPVTAVDVATKYNVITKEFNTKLPLIIDGNVSS
                     EQLDIKKPINIQIIIYLIATILVIIIIILIIGAINNYKNKKIDIRPDSKKMMYDYFGK
                     DGKEKQKANGPQEPSKTENTENKTQTAEEKTKKSSESDKFNEDYQYILSAIKKR"
     gene            16631..17353
                     /locus_tag="PLK55_00865"
     CDS             16631..17353
                     /locus_tag="PLK55_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB domain-containing protein"
                     /protein_id="HOZ35518.1"
                     /translation="MIKELDFNEKHITIVGTAHISAKNRDQVKDIILEKKPTAVCVEL
                     DANRLYALMHPEEQKKPKFSNIFKTKHKSVFLLSYILGGLQKNIAKNFNITPGEEMLS
                     AISGAKEIGARIYLVDRDINITLQKLLKRLTFGEKMKVLFGGLFIPKKELKKIDINKL
                     MSEVETEEEKSEIIEEIMKLFTKRHKKLKEILIDERDEFIAYNLQKIPEENIIVVVGA
                     GHMNGIINNLNKEIDIKKILTI"
     gene            complement(17346..18164)
                     /locus_tag="PLK55_00870"
     CDS             complement(17346..18164)
                     /locus_tag="PLK55_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOZ35519.1"
                     /translation="MIKAKTIKKVSKPEVKKKAKAKSKPHKDAHTYFSKTKISYPSEF
                     SKFDLHIHSKFSPDAVSEPETIIKRVKAAGLKGFAITDHNTIRAWPELARLAKQNNIV
                     FVPGQEIDVTFNGKKLGHILALFTYDKITSTEILEVVDDARSQSALLSFPHPFDTGRY
                     FRGFDYLMNNGPISRFVPAMETFNARVLTSRANEIAKEFAHKHGLIQTAGSDAHMPYE
                     IGNGYLIAKAQTELELLKEIKLGRVVVNGELVGMVPRIHTLGAYLKIITDKNFK"
     gene            complement(18640..19179)
                     /locus_tag="PLK55_00875"
     CDS             complement(18640..19179)
                     /locus_tag="PLK55_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOZ35520.1"
                     /translation="MLFIAEEFILDIFLFFIPFYFIFLFSRANSFSQVFRLQGLKKIS
                     FKELFKKTIIVFLLLFALSYTLSFFSVLFKVSDLNLVGEEILKYSPLAIVYLFLIRVF
                     LEEWFFRGFLTPRLGVILSSAFFAIGHLAYGSIIEIIGAFVLGIFLSSIYKKYNNLWP
                     NIFAHVLYNLVIYILLISF"
     gene            complement(19204..19428)
                     /locus_tag="PLK55_00880"
     CDS             complement(19204..19428)
                     /locus_tag="PLK55_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HOZ35521.1"
                     /translation="MGKKKGGGVKKGKTKHKPTVNIKVSKFYSVSGDTVDRKGKECPK
                     CGTGVRMGLHKSKEGKTRYACGKCGMNVFE"
     gene            complement(19435..19743)
                     /locus_tag="PLK55_00885"
     CDS             complement(19435..19743)
                     /locus_tag="PLK55_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35522.1"
                     /translation="MNIEIEKQRNELLHRTAIIAKVKGAKTPSRKELLKKVSAMLGAD
                     EKLLVVDKISQESGKQSAVAYLKLYDNAKILKELELNYKIKRTGDLEEKKEEKKKDEE
                     "
     gene            complement(19757..19975)
                     /gene="spt4"
                     /locus_tag="PLK55_00890"
     CDS             complement(19757..19975)
                     /gene="spt4"
                     /locus_tag="PLK55_00890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013143915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HOZ35523.1"
                     /translation="MVYKACRKCRKIVEDDIADEDKSSIKECPVCGNTTFTTFWKGNA
                     LIIDSENSDIAKAMEVKTPGRYALRLSR"
     gene            complement(19982..20611)
                     /locus_tag="PLK55_00895"
     CDS             complement(19982..20611)
                     /locus_tag="PLK55_00895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006333.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HOZ35524.1"
                     /translation="MGYKKYTIEGIVSIAPQRLSGELKDIILDQLCNDFEGIIDKNIG
                     VIVAVTEVISHDIGEIVPADPNVFFKVKFSVVSYLPKLHEIVSGNISQATDFGAFIKM
                     GPFEGLCHVSQVMEDFNSYNAELPGFSGKESKQTLVVGDEIIARIANVSLKPSIADTK
                     IGLTMRQFGLGKPEWQKAEKKKEKKVVKKTTGKGKEDKADNKSRSKKKK"
     gene            complement(20624..21031)
                     /locus_tag="PLK55_00900"
     CDS             complement(20624..21031)
                     /locus_tag="PLK55_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ35525.1"
                     /translation="MHKFDRVANIIVDTNVFIYMYELKRDIFEFALTVLPNAKFFVLD
                     ESFKEIEKVYKDKPQKLKTLKNYIEKLKELNKFEILAVDKNLIERYKKVDNLLIYFSN
                     KYIIYTNDRILKAKIKKKGNRVLTLRLHDVFLN"
     gene            complement(21015..22205)
                     /locus_tag="PLK55_00905"
     CDS             complement(21015..22205)
                     /locus_tag="PLK55_00905"
                     /inference="COORDINATES: protein motif:HMM:NF003077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HOZ35526.1"
                     /translation="MENIQSVLNIGVVGHVDHGKTTLTSKITGTWTDKHSDEIKQGIS
                     IKLGYADACFYKCKTCQGLQAYGVTNKCHLCGGERELVRKVSFVDAPGHETLMAVMLS
                     GATLLNGAILVVAANEECPQPRTIEHFSALKMHGLKNLVVAQNKIDLVTKEEAQENYK
                     KIRKFLDDEGYSHVPIIPTSAHFDANIDALVYALLTAIPVPELDTKAPLKMRIVRSFD
                     INKPGNPVEKIKGGVLGGAIMQGTLSEKQKAWLYPGINKPIEITIESLNSNGISFKQV
                     HAGGLIAVGTTLDPFYIAGDKMIGQLICEESSKPLINQELELKYIKIERLLESNKEDI
                     KLGENVVLIIGTAAHLGTIKKIKGDIVSISLKNVAVYYKDETIAISRLVSGRWRLSGY
                     AQIR"
     gene            complement(22294..22704)
                     /locus_tag="PLK55_00910"
     CDS             complement(22294..22704)
                     /locus_tag="PLK55_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HOZ35527.1"
                     /translation="MRLTLSDPKTKKSYNAEADEKTKLHLLGKKINDEVDLSNISPGL
                     KGKITGGANKQGFPMHTKLDSEVSKKILTTKGIGFRTKRSGERRRVRVAGRVVVAGTA
                     QVNIKLTAGDTTGFFEKFPKQKKEEDSGAKKKKK"
     gene            complement(22723..>23876)
                     /locus_tag="PLK55_00915"
     CDS             complement(22723..>23876)
                     /locus_tag="PLK55_00915"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35528.1"
                     /translation="VNGWGLAEAVLFLSVLSQKFLGKKITIEESDYMHGAVLEIGDLK
                     GMGSTADVIVYQGSLKLKDEIVFPTRFGLQNSKVKAILKPNFMTAVDKKGIFENISEI
                     HAASGIKVVAPGIEDCLPGAVIISASDKEAVERLKTKSYNCFIKGSEGAFVKADTLGS
                     LEALHKLLEKECIKIASSDIGEFTNKDLTELKILNEKDKIQGVLFLFNVKINQEFEDE
                     ITRLQIPIFKNNIIYKLIEDYQEWLGKTKKKELDKLLKEIIYPCRFKVLPNFIFRSSK
                     PAVVGVRIIEGRLISGGAVSLNDQKIGTIEGIQSDGKEINIAQKDMEVAVSIGDATYL
                     KDFKEGDVLEISLNLDMINKLEALEGELSPEEQTMIEERKNEIYKKQNQ"
     assembly_gap    23877..23976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23977..24593)
                     /locus_tag="PLK55_00920"
     CDS             complement(<23977..24593)
                     /locus_tag="PLK55_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOZ35529.1"
                     /translation="MGHIDSGKTTFLDKIKGTSIVDKEAGKITQHIGATEITIEVIQK
                     ISGHLAKKYNFELSIPGLLFIDTPGHNAFDNLRERGGSLADLVVLVMDINKGLQVQDI
                     ETLNILKMYKVPFLVVANKVDLVQGWYQEGKDITNNLSTQSKHTEEKLDELIYRIVGQ
                     LYDKGFQAERFDRITDFTKQIAIIPGSCVNGWGLAEAVLFLSVLS"
     gene            complement(24682..24885)
                     /locus_tag="PLK55_00925"
     CDS             complement(24682..24885)
                     /locus_tag="PLK55_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007044372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HOZ35530.1"
                     /translation="MKCTFCGKEIKKGTGIIYAKKDGTTSHFCSSKCKKNQLVKKYKP
                     LKTRWTDTYHLEKTRHQKKLAKE"
     gene            complement(24903..25112)
                     /locus_tag="PLK55_00930"
     CDS             complement(24903..25112)
                     /locus_tag="PLK55_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HOZ35531.1"
                     /translation="MPEEGVPAEIVEIVGRTGSRGEATQVLCRILDGYEKGRIKKRNV
                     RGLVKKGDVLILMNPESEAKDIRGR"
     gene            complement(25123..25686)
                     /gene="rpl7ae"
                     /locus_tag="PLK55_00935"
     CDS             complement(25123..25686)
                     /gene="rpl7ae"
                     /locus_tag="PLK55_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HOZ35532.1"
                     /translation="MADYVKFSVPEDLKNLQADVVSKISKSGKIKVGINEVTKMVERN
                     TAKLVLIAEDITPAEIVMHLPIICKEKNIAFSYVSTKEELGKISGINAKASAIAIVDE
                     GVLKKEFSALISKIEELSGGKSKAEDKPKPAEKKPVAKKEEPKVEKKEEPKVEKKEKP
                     KVEKKEEPKVEEKPVAKKEEVAEEKTE"
     gene            25804..26232
                     /locus_tag="PLK55_00940"
     CDS             25804..26232
                     /locus_tag="PLK55_00940"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOZ35533.1"
                     /translation="MSLNTIKNALITEEKKSLCLRKQIGAVVVKNGKIIGSGHNGNSS
                     NIPPCSVIGCAKDYFKFRPGLRNELCTGICAEQRAMIDAIKNGHNLKGAELYSTYSPC
                     VSCVRYMIEFGIKKVFYKNDYRDAFARQIQKLGKIEREKI"
     gene            complement(26238..>26402)
                     /locus_tag="PLK55_00945"
     CDS             complement(26238..>26402)
                     /locus_tag="PLK55_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOZ35534.1"
                     /translation="DFNVSKVTNMGGMFNNASSFNQDISSWDVENVTNWSEIFTNCPI
                     QTDYKPEKFR"
     assembly_gap    26403..26502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26503..28489)
                     /locus_tag="PLK55_00950"
     CDS             complement(<26503..28489)
                     /locus_tag="PLK55_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOZ35535.1"
                     /translation="MGQKLIRNRETLSKNNKPQKTDLKRKAQATMEVLIIMGVLVVGV
                     IIFGTFYFGNIGERSRGSAGISRDMNESELKTSYDKAIIYDGVDQVSMDGGSSHIVPD
                     DGDSDDEDGDEPPGSEETGPPTILVFDTRNTSDGSSGSPSPSSTGNQIGSTLEDGSPL
                     EGIDTYNFSVDWNDDTDSNITSLNQPKATPTNSDAGNLPSSDSSQIRLPLESSGTYNF
                     NVDWNDGTNSHITSWNQPEATHTYSEPGIYEIKITGVIKGFRFNNNGDRLKLIEIKNW
                     GNLNLGNNGYYFYGCSNLDITATDVLDLNGTTNLEGIFYKCSSLVFNESINSWDTSKV
                     TNMGSMFNMASSFNQPLSFDTSNVINMSFMFSNASSFNQPLDFNTSSVTDMFAMFQCA
                     IDFNQPLSSFDTSNVINMRNMFGCIYDMSFNQPISNFDTSKVGNMSYMFRNASSFNQS
                     LSNFDTSNVTGMQHMFRNASSFNQPLSSFDTSNVTDMWGMFSGASSFNQPLSSFDTSN
                     VTNMRSMFSGASSFNQSLSSFDTSNVTNMGAMFFDAHSFNQSLSSFDTSNVTDMYGMF
                     SGARSFNQSLSSFDTSNVTNMHWMFRDASNFNRLLSNFDTSNVTDMSEMFRNASSFNQ
                     PLDFNTTNVTDMSGMFSDATSFNQPLDFNTTNVTDMSG"
     gene            complement(28629..29354)
                     /locus_tag="PLK55_00955"
     CDS             complement(28629..29354)
                     /locus_tag="PLK55_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ35536.1"
                     /translation="MQNSKILSLLLDFGLTEYEARTLTTLFNFVEADAPHISRNAEIP
                     KTRIYDVLEKLKEKGLVLEVYGRPKKYKVIDPNEIFKYLIDNKQRELSVLDKQVKDVL
                     SKETWANQVSGVQEKILQVKNIKDYNKLLSQEFEGAKKEITGFTNIDDRVDAFKPVSE
                     MPEINVKLISKPITKDFDLPKNFSIQEKEHSMDAFIIDKNKVVMSLNDLSSPKETYHL
                     TILKNNPSLTKALLTHFDDYWKK"
     gene            complement(29413..30339)
                     /gene="rpsB"
                     /locus_tag="PLK55_00960"
     CDS             complement(29413..30339)
                     /gene="rpsB"
                     /locus_tag="PLK55_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOZ35537.1"
                     /translation="MDKVNAKPRNFENENRTPSQSRTLVNVDLYLKNGIHIGTKYKNG
                     PMRKFVFKSRNDKINVMDVQIIDKRIKIAIDFISKYEPTDVVFISRKKYALPGVKILE
                     QAFGYKSKLNRFIPGTFTNPESEHFIEPKLVFIADPNVDRQAVVESTKANIPIVALCT
                     TSSNTRNIDLIVPYNNKGRKSVALFFWLLIRELELRNGIIKSDKDYAYSIDDLVYNSE
                     NGEKEMDEDQPLPGKRVRKVSTKATARKPAAGGRRPSSTTGSAPRRPYVRRTPLVAAM
                     PIATAAAPAATTAAPTVENEKKEEKPAEVSKE"
     gene            complement(30351..30545)
                     /locus_tag="PLK55_00965"
     CDS             complement(30351..30545)
                     /locus_tag="PLK55_00965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HOZ35538.1"
                     /translation="MICPVRCFSCGQVVADKYDEYVRLITQEKKTPEEALNILGIDKY
                     CCRRMLLTHVEVIDEIMKYN"
     gene            complement(30550..30951)
                     /gene="rpsI"
                     /locus_tag="PLK55_00970"
     CDS             complement(30550..30951)
                     /gene="rpsI"
                     /locus_tag="PLK55_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOZ35539.1"
                     /translation="MVKNYFVKASRKNAIARCMSKAGTGKVRVNGHSLEAYYSGYKQG
                     LIKEPMILLDEEANKLDYFVNVSGGGVMSQVQATRSCIAKSILAANNREKVKDKIMTY
                     DRHLLVDDVRRKEPKHQLGKGARKKKQHSKR"
     gene            complement(30968..31387)
                     /gene="rplM"
                     /locus_tag="PLK55_00975"
     CDS             complement(30968..31387)
                     /gene="rplM"
                     /locus_tag="PLK55_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOZ35540.1"
                     /translation="MIVLNAKNKILGRLASEIAPKLLRSSEKIIVVNANDAFISGTKE
                     DIMARFKERTHRKGKGNQLKNAKYPRYPDKMIKFAVRGMLPRNARGQAALKNLKVFVD
                     APEAYVKDMSKEVVEKPKLKGMNLNEICVLLGAKLKV"
     gene            complement(31409..31762)
                     /locus_tag="PLK55_00980"
     CDS             complement(31409..31762)
                     /locus_tag="PLK55_00980"
                     /inference="COORDINATES: protein motif:HMM:NF003079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HOZ35541.1"
                     /translation="MKKDTKNLQTQKLIENLYKLSKDKKKEIYVSVAKILEASSKKQP
                     SKNLFNLQKMSYINDGDIVVIPGKILGTGILEKKITIYALSFSESAKTKYKGLKTLRD
                     FCKDNIDYKKVKLIK"
     gene            complement(31922..32003)
                     /locus_tag="PLK55_00985"
     tRNA            complement(31922..32003)
                     /locus_tag="PLK55_00985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31967..31969),aa:Leu,seq:taa)
     gene            complement(32025..32762)
                     /locus_tag="PLK55_00990"
     CDS             complement(32025..32762)
                     /locus_tag="PLK55_00990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HOZ35542.1"
                     /translation="MAKITYLETVGKTYKYLAEGFDPIDMNTLRKKISYCLSVYAVDE
                     IDFYENDSVIVDEMLANRIALVPLTTPTESTGKKVTLTLEKDGPCTVYSGDLQSNDED
                     VKPVYDTIPLTKLKENQRLRLDAYAIQGQGRTHVKFSPAIVSFKQLPELEILRGCDGC
                     EACVNACPVKCMKMVSGKPKIDDVNKCTNCQACVDACPKSCLKLNDTNNYILNIELIG
                     QLPIEAIVKLLDRYTKEYMMLLKKKFK"
     gene            complement(32781..33176)
                     /gene="rpsK"
                     /locus_tag="PLK55_00995"
     CDS             complement(32781..33176)
                     /gene="rpsK"
                     /locus_tag="PLK55_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOZ35543.1"
                     /translation="MIMAGIGKTGIVNIFASRNNTIIVLTDETGSEVIAKCSGGQMTK
                     SQHKEGSPYIATKIAQVVAEKANDKGIKNVHVKIRAPGGNLYYNPGQGAESSLKTLTR
                     AGLNILSISNTTPVPHDGCRKQKKFRKKK"
     gene            complement(33173..33739)
                     /locus_tag="PLK55_01000"
     CDS             complement(33173..33739)
                     /locus_tag="PLK55_01000"
                     /inference="COORDINATES: protein motif:HMM:NF003139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOZ35544.1"
                     /translation="MGEPRRLNRQYDVPRKLHDKQKMIIDRKLEREYGLKTKREIWKA
                     ESVVRNYRKMARKLLAGNVDNYEKRNSELIGKLQRTGIFTKDHTIDDVLKLQASDLLE
                     RRLQTQVFRRGLALTPKQARQLITHGHIIVNGTKRTAPSSLVMAGDSIAYIDTKIKAA
                     IDRAQEQKGKPFVKPVKQNKEEKKEESE"
     gene            complement(33771..34361)
                     /gene="rpsM"
                     /locus_tag="PLK55_01005"
     CDS             complement(33771..34361)
                     /gene="rpsM"
                     /locus_tag="PLK55_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOZ35545.1"
                     /translation="MAKDKKINNTPELKVKKSRSEGSTHISADKLKMVKPKPKPSHFN
                     PAKEGADFRHIVRIANRDIPGYMPISDGLTLIYGISKRLGKVIEEIFYKDSGKVINRV
                     GNLTEKDVEAIEKIIADLDKRVPAWLLNRPKQRDGTQAQYIMADLKLVERKELQRLGK
                     SKSYRGLRLQWGLRVRGQKARSTGRRHSTIGVEKKK"
     gene            complement(34378..34536)
                     /locus_tag="PLK55_01010"
     CDS             complement(34378..34536)
                     /locus_tag="PLK55_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35546.1"
                     /translation="MGLLEKGRKCIIMQGRNIGKQIVIDKMDTKYIYYKIKDKEQKIG
                     ILHVFPLE"
     gene            complement(34543..34884)
                     /locus_tag="PLK55_01015"
     CDS             complement(34543..34884)
                     /locus_tag="PLK55_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35547.1"
                     /translation="MPNPNQKFKKHKTTITPSGKKSRVLVKKKTSKHKCAVCKAILHG
                     MPHGKRTVEVKRLSKTERRPENLLSSVLCPKCRQIAYLDAVMLKHNIKTEEDVDLRYI
                     KYIKMILKKIE"
     gene            complement(34902..35381)
                     /locus_tag="PLK55_01020"
     CDS             complement(34902..35381)
                     /locus_tag="PLK55_01020"
                     /inference="COORDINATES: protein motif:HMM:NF021490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2208 family protein"
                     /protein_id="HOZ35548.1"
                     /translation="MNIPITGSHWIDLILLALIFVILNRAIQHFFSDPKEYFFVKRRN
                     KEIQAEMKVLMKENNYIAVQEKQKEAFSLMGRQFKGMFKTMIPLMIMAIPFLYVINKF
                     YGAIKFIDTTFPWTFDLHGFWTYFIMTFIFSIIANNIYDKIFEQKYKDYVPADYKKK"
     gene            complement(35388..36722)
                     /gene="secY"
                     /locus_tag="PLK55_01025"
     CDS             complement(35388..36722)
                     /gene="secY"
                     /locus_tag="PLK55_01025"
                     /inference="COORDINATES: protein motif:HMM:NF006341.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOZ35549.1"
                     /translation="MSFFDGILRALPSVKEPSKVPNVRQKLLWTLVVLVIYFILGTIT
                     LFGLDPSQTANISMYQMILASDIGTLITLGIGPIVLASIILQLLAGVKIINFDFSKSE
                     DRAKFSGAQKLLAIVLAIVEAIIYSAFGMLVPAQGMLLIVILQIVIGAIIVIYLDEVV
                     SKYGIGSGIGLFIAAGVCQAIIWQIFSFPNSAISYPGGLLFQGISYAATGAMTELLAT
                     VLLPILFTLLVFFAVVYVESIHVNIPLTVGRSGMGANFPVKLMYVSNMPVILTGTLFA
                     NIALFASLAKGKALGNFLTPVANFLNPSQGLLQTAIFGGATWGTVLSVLGYSILFVLM
                     CVIFGKFWVEVGGQGTENVAGQLQRSGMSIPGFRRDERVLVTVLNRYIPTIVIMGSIT
                     VGLFAVFADITGAIGTGTGILLTVGIVYRFYEQLAKDQLFESGNFLKTMLNK"
     gene            complement(36719..37087)
                     /locus_tag="PLK55_01030"
     CDS             complement(36719..37087)
                     /locus_tag="PLK55_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HOZ35550.1"
                     /translation="MVKRKIDRLRGKRTHGHGNTKNARGSGCTGGKGRAGSTKHKFTK
                     YYVDFGQRIFLKPKTKLKTITLEALSDMIKDKKEINLKELGYDKILGKGNITKPVVFN
                     NALVTAVAKEKIEKAGGKVQ"
     gene            complement(37102..37566)
                     /gene="rpmD"
                     /locus_tag="PLK55_01035"
     CDS             complement(37102..37566)
                     /gene="rpmD"
                     /locus_tag="PLK55_01035"
                     /inference="COORDINATES: protein motif:HMM:NF004711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOZ35551.1"
                     /translation="MFAIIRIRGPAKVNVNIENTMKLLNLTRVNHCVIYPEDKYVKGM
                     LHKLRNMVTYGEISKEALKKMLNKRGYCYNEKGKLIKFKEHYKDEKKQDKVIEEIVSG
                     KAKLEDLGIKKVFRLKPPTKGFDRKGIKKTFKEGGVLGYRADQINTLLLKMI"
     gene            complement(37582..38283)
                     /locus_tag="PLK55_01040"
     CDS             complement(37582..38283)
                     /locus_tag="PLK55_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOZ35552.1"
                     /translation="MCAFSERERRPRREKREEKVFDKDAWIPKTELGKKVKAGELASL
                     DDVFKLGKKIMEPEVVDCFISDLKMKVIDVKKTTRVTRAGRHFSFRVSVIIGDGNGHI
                     GVGTGKNIERIVAQEKAIKDAKLNIVPVRRGCGSWECNCSKKHSIPYKVIGKCASLRV
                     ELLPAPNGIGLAVSDTIKPIFDLAGIKDLWSNTRGATDTKLNFTRATIDALYKISDLK
                     TKEKEIETEDKENKE"
     gene            complement(38291..38803)
                     /locus_tag="PLK55_01045"
     CDS             complement(38291..38803)
                     /locus_tag="PLK55_01045"
                     /inference="COORDINATES: protein motif:HMM:NF006342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOZ35553.1"
                     /translation="MTSGPTFKVAFRRRREQKTDYKKRLAVLKSKKDRVVLRVTNALV
                     KAQLIRYDKDGDKTLASADSKELKALGYSGNTKNTPAIYLTSLLLAQKAKKKGVKEVI
                     LDLGARNYKSGNKIFAGLKAIVDSGLDCPHDEKAFPGKDRIEGKVMAEYLKKPIDKEF
                     IVTKEKILKM"
     gene            complement(38800..39252)
                     /locus_tag="PLK55_01050"
     CDS             complement(38800..39252)
                     /locus_tag="PLK55_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HOZ35554.1"
                     /translation="MKLDKLKTFSGKILKKSKHKLVIDEKKYQDNKEFVDKAITKQDV
                     RDAIAKGVIKKKNDTGQSRGRARVLLKKKQLKRKRGQGKRKGTPKARQSRNLYNDKIR
                     ALRKRLRELRKEDKLNNKNYREIYQRINGNYFRGKKHLEEYISSGEKK"
     gene            complement(39269..39607)
                     /locus_tag="PLK55_01055"
     CDS             complement(39269..39607)
                     /locus_tag="PLK55_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="eL32 family ribosomal protein"
                     /protein_id="HOZ35555.1"
                     /translation="MSNDTPNFRGHFPLTLRRTKHNRKVQKWRYPRGIDRHMSVKKGP
                     QPKIGYGHKNTEKGMHPCGLKECIVRTVTDINKLNPKEIAIRFNGTLGLKKRTELRKV
                     AKEKGFKILN"
     gene            complement(39610..40167)
                     /gene="rplF"
                     /locus_tag="PLK55_01060"
     CDS             complement(39610..40167)
                     /gene="rplF"
                     /locus_tag="PLK55_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOZ35556.1"
                     /translation="MQAKKEKKEIKETKIKIPGDVKVHLGSHDLILEKSGKKTDVEFN
                     PVYVSAKFENGEIELIPKSKKKTIKAVVNSTEKHIINALKGFEKDYVYKLSVVYSHFP
                     ITVKVEGHEVVISNFLGEKKPRKTRILPGCKVEVKGKEIIVKGHDKYAAGQTAGNIEK
                     ATRVVGRDFRIFDDGCYIVEKAKRE"
     gene            complement(40191..40580)
                     /locus_tag="PLK55_01065"
     CDS             complement(40191..40580)
                     /locus_tag="PLK55_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOZ35557.1"
                     /translation="MSLNDPISNALNNINNAEMASKQTCTLNHSKFLESVLTLLKKEG
                     YIKMYKVLEKKPCKQVVVYLGGRINKCKAVKPRYPVKKQDYEKFEKSYLISRDVGVLI
                     VSTTQGIMSHKDAKEKGLGGRIVGYIY"
     gene            complement(40602..40775)
                     /locus_tag="PLK55_01070"
     CDS             complement(40602..40775)
                     /locus_tag="PLK55_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOZ35558.1"
                     /translation="MSEIDYKKLGKTSKELKRTRKCRFCETNKGLIRKYNLYICRRCF
                     KDYADKLGFKKLD"
     gene            complement(40772..41296)
                     /locus_tag="PLK55_01075"
     CDS             complement(40772..41296)
                     /locus_tag="PLK55_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOZ35559.1"
                     /translation="MTTDKNNNPNREIFIEKVTVNMGIGEAGEELEKGLKILQLVTKK
                     KPVKTMATVKLPTWGIRPGLPIGGKVTLRGKEALDFIDLTLKAKEYKIKKKSFSNEGH
                     FSYGIKEYLDVPGIKYDPNLGIRGFDVCVSLKRRGYRVKQRKYNIAKVGKKHRITKDE
                     AMEFAKEKLKIVVE"
     gene            complement(41310..41999)
                     /locus_tag="PLK55_01080"
     CDS             complement(41310..41999)
                     /locus_tag="PLK55_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35560.1"
                     /translation="MTKKGSTRVLKTQNVGKVHKVDKKKTFVLRPDPGKHTLLKVVSA
                     GYVLRDILHLTENNRESTFVVRNKEMAVDKKPIKSINYAVGLFDIIELPKIKKAYKVI
                     YKQNGLITLIEINQDETKYKVCKIIKKMITKKGQIQLITNDGRSVITTNQAYKTKASI
                     KLDLEDNTIKEYYPLEKGRKVFLIGGKHIGKTGIIVAVKKGNMIKKELVKLKIDDKEF
                     ETTEKNVFVIN"
     gene            complement(42015..42377)
                     /gene="rplX"
                     /locus_tag="PLK55_01085"
     CDS             complement(42015..42377)
                     /gene="rplX"
                     /locus_tag="PLK55_01085"
                     /inference="COORDINATES: protein motif:HMM:NF028216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOZ35561.1"
                     /translation="MINKTKKPGKNRKRMLTGVYHVKRKLLVAPIDKQLVKQVNKKRA
                     SLRKGDTVKVLVGDNKGKTSVVEKVNYDKGTVYLKDFKVTNSRGQEKLLPFKASNLVI
                     TTLVLNDKKRFVEKKSKK"
     gene            complement(42399..42797)
                     /locus_tag="PLK55_01090"
     CDS             complement(42399..42797)
                     /locus_tag="PLK55_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HOZ35562.1"
                     /translation="MKPISAKVTRGLQIGSRCLCSDNSGAKEIEIISVINIKTKRKGV
                     QSAGIASLLNVVVKKGKPAMKKKMERAVVVRVRKEYRRKNGWRVCFEDNAVVLTDPKG
                     FPKGSEIKGCVAKEVGERYPKIAGLASAVI"
     gene            complement(42794..43165)
                     /locus_tag="PLK55_01095"
     CDS             complement(42794..43165)
                     /locus_tag="PLK55_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOZ35563.1"
                     /translation="MDAKTCLDKNCPIHGQISVRGKTLQGIVVKKKAIKTAVIQMEKT
                     KYIRKYERYMKKKVKIPVHCPECMEINIGDIVLVGETRKLSKTKSFVIMKKLGTVSHM
                     EDIIKDDDKYEGIEEEEVEEE"
     gene            complement(43172..43366)
                     /locus_tag="PLK55_01100"
     CDS             complement(43172..43366)
                     /locus_tag="PLK55_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HOZ35564.1"
                     /translation="MLNVKNSELIGKIIIIKNSSDRTKIGKKGIIIYQTKDLLKIRDD
                     KEKIFEIKKNEIIDLETINM"
     gene            complement(43359..43661)
                     /gene="yciH"
                     /locus_tag="PLK55_01105"
     CDS             complement(43359..43661)
                     /gene="yciH"
                     /locus_tag="PLK55_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HOZ35565.1"
                     /translation="MAEICPVCHLPKTICTCDLRTREQQQIKIRTERKKFKKYATLIS
                     GFTDANEQQEVFKYLKKKLACGGTVKENVIELQGEQKERIKKLLIEKGYLGELIDA"
     gene            complement(43685..43951)
                     /gene="rpmC"
                     /locus_tag="PLK55_01110"
     CDS             complement(43685..43951)
                     /gene="rpmC"
                     /locus_tag="PLK55_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOZ35566.1"
                     /translation="MTKKKAVALNSMVEIENKVRDLKLDLAKEKGMLASKTKSTNPSK
                     KTTLKKQIARLLTKLNALKKKQKEVPVKVASKKEVTPKTKDKKK"
     gene            complement(43956..44771)
                     /locus_tag="PLK55_01115"
     CDS             complement(43956..44771)
                     /locus_tag="PLK55_01115"
                     /inference="COORDINATES: protein motif:HMM:NF003219.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOZ35567.1"
                     /translation="MKENVFVKDKLKKMAIAQYLQKKLKQAGFIDVEVVKSPISTRVV
                     IYALRPGFVIGKSGFNIKALTEDLERDFGLNKVHIEIGELQNKDLDPRVIIENLSNDI
                     GRGIAWKSAVYRAMTQMKNAGAVGAEIVAKGNTAGKGQRKRSSRYAFGYLKKAGSQKD
                     LVAFEKRTTFPGLGTIGIRLRIVQPETVFSDKIDVRELSHRHKLAREQKRLENREEEV
                     EKETIVAKEKVEEKKKAKEEKSTEKTEKKPRVKKEKKETKEEKLEIVEEIIEE"
     gene            complement(44780..45253)
                     /gene="rplV"
                     /locus_tag="PLK55_01120"
     CDS             complement(44780..45253)
                     /gene="rplV"
                     /locus_tag="PLK55_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOZ35568.1"
                     /translation="MSSDHNYNYQKKLDSKKSARASKHNCPISVKYAVEFGNYFRNMP
                     VKKALKILADIEAKKDYLPLVKYAKKVPNRKGQSKRGTTIGRYPVKCAKYVRLMLEEA
                     KANAENKNLDTEKLKVESMFATKGVTRQKIQPMGRIGGKTRESKSTNIEVIVVEA"
     gene            complement(45265..45648)
                     /locus_tag="PLK55_01125"
     CDS             complement(45265..45648)
                     /locus_tag="PLK55_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOZ35569.1"
                     /translation="MAEEFKYRGHTLEELKKMSLLDFANIAGTRAKRHLRRNHSDAFY
                     ERIEKDKKANKKIRTHRRDIIITPKMVGKTIYVYNGKEFLQTEIFPDMLAHYLGEFAF
                     TRKRIRHGKAGIGATKSSTAVAARK"
     gene            complement(45674..46378)
                     /locus_tag="PLK55_01130"
     CDS             complement(45674..46378)
                     /locus_tag="PLK55_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOZ35570.1"
                     /translation="MGRRLKIQRRGKGGPVYTATNNAKFDINYRTVKELQTGQVIDLV
                     TDPIRSSPVAEILMADGTKEYVICAEGLVTGQRLEFGSGAQKKKGNVLPLGEIEPGTT
                     IFNIEKNFGDGGKFVRAGGQTAILVNKKEKAIIKLPSGKLVQLPLDARATIGVIAGGG
                     RKTKPFVTAGAKFKAMKAKGKKYPRVRGVAMNPVDHPFGGSQHHVGHSKSTSRHAPSG
                     RKVGAIASKRTGRKKK"
     gene            complement(46393..46662)
                     /locus_tag="PLK55_01135"
     CDS             complement(46393..46662)
                     /locus_tag="PLK55_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOZ35571.1"
                     /translation="MKKIEYYEVLEYPVITEKTVNMISTTNRVTFVVNKASTKKEIKE
                     AVEKLYNVKVKTVNVLFDRKNRKKAFVTLKKGFNAQDLANKLGII"
     gene            complement(46673..47479)
                     /gene="rpl4p"
                     /locus_tag="PLK55_01140"
     CDS             complement(46673..47479)
                     /gene="rpl4p"
                     /locus_tag="PLK55_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOZ35572.1"
                     /translation="MKANIYDLEGKTIKEIELPEYFAQDYRPDLIKKAVISSQSKGYQ
                     AMGVSPYSNRNYTSIYIGRRKENKANITINTGHARLPRTKNKRVLMAGRVMRVSQAVG
                     GPRAHPPKVAEILARKINSKERTKAIISAVSATANRNLVEERGHILPEKITLPIIFDN
                     KLESLSKTSQIYTALEKLGLDKDVIKAKEKKTIRAGKGKMRGRKYKKRKSILFVLADS
                     KNYKAFSNLEGADVISARQLSIKELAPGTHAGRLTIYSQAAIEELKKRFE"
     gene            complement(47502..48488)
                     /gene="rplC"
                     /locus_tag="PLK55_01145"
     CDS             complement(47502..48488)
                     /gene="rplC"
                     /locus_tag="PLK55_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOZ35573.1"
                     /translation="MGVQNKPRSGSMSFYPRVRASSIIPKFNSFGGVEKTIKGTKPLC
                     FYAIKAGSTFVIGKNAKKGASSYGQEISVPVTIIETPDLKVIGARFYKEHNVNAGKKA
                     IIEFTNQDTDFKKRVTGRKQKKSATLEDALKRKDEADSLVLIATINYKATGTGQKKPV
                     IVEIPLSGTYDEQISYLKEKINKTISVDDVFKVDDYLDVKSITTGYGTTGVVERFNVK
                     VQRRKANKSQRHVGGINPWHPATIMYTVARAGQYGYHNRTETNKKLVMIDSDINKVNR
                     KGGLKNYGLLKNKYILVDGSTPGTTKRVVALRHTFRPQKQKLELTDISYINK"
BASE COUNT        13955 a         8821 c         6600 g        19252 t
ORIGIN      
        1 tttaagttaa ttaattaaat aaatataaaa aaagagaacc gatatatata tatatatata
       61 tccttttgtg agcccgagat cgccttaacc aaatcgaaaa actaattatt caattaacca
      121 tttccaaagc ggaatatatc taattatttt gttattaatt ttttcttctt tttctaaatc
      181 gttagttatt atagttccgt cttttaatcc aaatttgtca caagtatata aaagtgattt
      241 taattctctt tcttttgttt ttgtatctgc caaatcataa caaacttgta ttgctgaaac
      301 tattttgtct ttgtctttaa ctaaaaaatc aacttcccaa ccattatcta acttataaaa
      361 ataaatcttt tttcctcttc ttaataattc taaaaaaaca atattttcat ataatttccc
      421 tagattttca gaaaacttaa aggccatttg attaattagc ccggtatcta tacaatatat
      481 tttttttgct gctctttctt gattttttaa agatggatca tatttattta actcaaataa
      541 aacataagca tttactaaat aggaaacata tttctttgcg gtgtgtgtac tgcccatatt
      601 aaaattattt tttaaagaag tatatgaaaa ctctttagaa aaattagtta ttaaaaagtg
      661 agctagtttc ttaaatgttg tttcatcaat tatcttatat ctatttatta tatctttatt
      721 tattacatca ttatatattg aacttaaaat ctcagaatta tcataaacca ccacttctgg
      781 gaatccccct ttctttatat attcttttaa atgcttattt atttcggtct ttccttccgt
      841 agttttagaa ttctttttaa aatccttata tcttaaatat tcttcaaaag aaaatggata
      901 tattaaaata tcaaaatgcc ttcctgtaag atatgtagag atttctttac ttaataattc
      961 tgcattcgaa ccagttataa aaaacctatg accttcagta tataatctat taacaaactt
     1021 atcccaattt ggttttcctt gtatttcatc aaaaaagaaa attttctttt ctccaaattg
     1081 ttctaataac acttcatgta ataactgaaa atcttttgtt ttaaaatcaa gtattctttc
     1141 atcagagaaa tcaaaataat aaaacttatc cttgtagtat ttactcatcc attgtaataa
     1201 aaatgtagat ttgcctgctc ttctgtgccc agttattact atgtttattt tatgatttat
     1261 aaatgatgtt atttctgata atttttgtct tattagccct tctgacaatt tttctaatct
     1321 tttgttttga tctaatatta tttgtgttaa atattcttta ttcatatata ctatagaacg
     1381 attctattta aaaagttgtg cattgccggt gcacaaatta ctgcgttttt gtgcatccga
     1441 agtgcacaaa atattgttac tcaaaatcat aacgcaaaag agaaatagac ttctctcttg
     1501 attagggttt gcttgcttgg agtttttggc tcaaagacct gataggagat tcttgttgct
     1561 gtaccttgct caattacttg ttttttgatc tttatgaatg cttcgtctga acaagagatg
     1621 tgaagagtaa ctacttgact tggagtaagg ttgtttttct ttctctctgc ttgtatagct
     1681 cttgttagtt cacttaattc ccattcgtct tctagacctt ctggcaaatc attgtcaaga
     1741 tagattgaaa gattatcgct gacttttcct tcctcaaagt ttccttttgg aatcgttgtt
     1801 tgaagaagaa cttctttaat gttagccata tttgctacaa tttctttgac gccagttaac
     1861 ttttctgcat ctggagagac aacaactaac cttggaagaa cccatctaag tcttttcttt
     1921 gtcctttctc ttgctgctaa ggcttcttga acaattgcta aagcaatttc aaagttgtct
     1981 tctagttctt tgttgattag cttttcattt gctactggaa gctcttcaag atgtattgtt
     2041 tcttttctat ctttaaactt taagaaaaga tattcagtta agtgtggagc tgctggtgag
     2101 agatgcaata acaatcctga aaagatatga ctcaagacat tgtttttagt ttctcttttc
     2161 tttattagct taatgtatgt tctactaaag tcttctagaa taaatttttc taagtctaga
     2221 gttgcttttg agaagttaca gccagaatag ttcttgtgat agctttcaac tagagaatta
     2281 tactttgaaa gaatccattt gtcttctatc tctagtttat cattatcaat tgagggatta
     2341 aatttctgct tatcatccat taatgccatc aaagaataaa tattttctag aagattaaat
     2401 actcttttga tatcagaaaa cttcttttca tcaattatta catccatacc attgtattct
     2461 ctagaaaaat aatatctgta gtaatcaatt gaggtttctg aaaatctttg tgctagcggc
     2521 ctgtcgtttc ctaaagattt agatagcttc tttttatcaa ctgctagaac cattccgtga
     2581 agagcaacaa actggtatgg tgctttgtca aaacagattg ctgaaacaag catctgagaa
     2641 ttccaccaac ctcttatttg gtcagagcct tcaagattaa tgtctggtgg ccagaactga
     2701 ttaaatgttt cttgtttctt tggataacca agacttgcaa aagaagcaat tcctgaatca
     2761 aaccaaacat caaagatttc tggaattctt ttttttgtgc ctccgcattt acaagcataa
     2821 gtgatattat caattcctgg cttatgaaga tccatattta catcaactgc tgaggcttta
     2881 atttttaatt cctctaaaga accaaagact tcaatctctt tacatttatc acattgccag
     2941 attggtattg gcgttcccca atatcttgct cttgagattg gccaatctga taaagagtca
     3001 agccaattat caaatctttc ttttgcccaa tcgggatacc aagttacttc tttttggttt
     3061 atttctctta gtctttcttt cattggctca attcttaaga accattgagg gagagcaacc
     3121 tgaagaagag gagaaacaca tctccaacag gttggatatg catgagttat cgaacgctta
     3181 ccaagtattg cttgcttttt ttctaaatat tcaataatct ttgggtcaag tgcttttact
     3241 ttttctcctt taaaaacctt cattgaagaa tcataaactc cttcaactgt tactggacaa
     3301 taagcaggga aattattttc tttgccaaca gtatagtctt ctctaccatg gcctggtgca
     3361 caatgcacaa gcccagttcc ttcctcaaga tgcacaaacc ttggagataa cgcaacttta
     3421 tagcaatcct tctttagctc atcaagcttt tctagccatt cgcctaagat tggttcatac
     3481 ttctttcctg ctagctcttg acctttgtat ttttttccta aatcataatc tttccaacca
     3541 agttctctta taatcttttc tactagttct tctgcgacaa tatatttctc gttattataa
     3601 attaattcaa cataagtaaa atttggatga accattattc ctagatttgc agggagagtc
     3661 cagggtgtcg ttgtccagat gacataatag gtgcttggca tatccagaga ttttagccta
     3721 acataaactg aagtgtctgt taagtctttg tgctcaatct cattaaaaga aactgcagtt
     3781 ccacaagatg ggcaaatatg tacaggatat ttatcgtgat ataaaagacc cttatcggta
     3841 gcctttttaa aaacccacca accagtctcc atatacgatt tatcaagagt tctgtagggg
     3901 ttcttaaaat ccatccattg tccaagatta tatatttctt ttgacatgtc gtcaatatgt
     3961 tctgttgcaa acttatagca ttcttttgta aagttataaa taccaaactt ttcaatatct
     4021 tcttttgatt ttaaagcata tttcttttga acctttgctt ctattggtac accatgcgta
     4081 tcaaagccag gggtatcaag aacagtatag cctgacattc gtctgtatct tatgattaga
     4141 tcttttagga ttctattcat tgctgttcca agatgtagac ttcctgaagc ataaggcggg
     4201 ccatctataa agtagtagtt cttagtgcct ttctttgctc tgaccttttc tggaattcca
     4261 ttgactttcc agtaattctt aatttctagt tctgttttgt ttgaaaattg ttcagccata
     4321 acttaatcaa atttatatat taaataatat attttagata gaaaaagttt ttaaaacaag
     4381 ttaatttaag ctatttcttt gtggctttgt caagaaggtt atcaacaaag ggaattatgc
     4441 ctttttcgtc ttgtttgaag tctattccgt tttgctctaa caaaattaaa acatcatcaa
     4501 tcattgcaac agagtaacca agagataaca tcatgctttg tagttcttct cttgtatatt
     4561 tattagcata cggccttatt aaaacataca agaagttaat ctgttggttt gtagaagtgt
     4621 tttttggtag gctttctttt gcttcttgag cagttagcct tcttgattct tttgatgctc
     4681 ctgcaacagg aatatttgca taatctcttc ttctttttat ctcattaata cttgcagaca
     4741 tatcttctgt ttgttttatt tctgataaat caaaagtttt atagcttact tttttctctc
     4801 tgaatttttc tacttttatt tctttgtcct ctaatgttga aacaaagttt tttagatagc
     4861 ttacttcttg atatatttct cttaaattat taaaatcttc agacattttc ttaacaaaca
     4921 attcttttgt ttttttcatg ttttttattt cttttatttc tgccttatat agctctttat
     4981 cttttaagcc ttctttaata atttcaaaca taatgtcttg agccactcct ttactaaaac
     5041 cattacttac taataaatca agtatccagt ctctatttgc accaatctct atcaagtatt
     5101 tgattgtgtt tctaatattt tgtattgacc ttttttgtat gctctctttt ttacttaatt
     5161 cttctttgtt tttctctaaa gcatattgcc tttcttcttt tgatctttct gtaatattta
     5221 aaacatcaag atagacgtgc tgtggtaatc ttaatattaa aaaaccctca ataaagaaca
     5281 acatataaat tactaaagaa actattattg gaacaatcat tggacttctt aaaaaaacaa
     5341 atatatcaag actaaagatt aaaatcatat ttagactaga caataatatt ccaattatcc
     5401 aatagataaa tcttatcttt gttgtttctt ttattatttt tacaagcctt tctgtagctt
     5461 ctgaatctag accaatataa tcagataatg ccttaactga ttcttttgaa tagttcttaa
     5521 ttaagatata gttcactatt cttctaatat tttttattat tagtttaagc tgtttttctt
     5581 tttgttcatc ttttgctatt ttttgaactt cttttaagtc tctgcttttt gaaagaacca
     5641 aatagaagat attagatatc tgagatacaa agatgcccac tccaaaaaac aaaagaaaaa
     5701 ttacaagaga tattgctaac ggaaggtagg ggttttttga agtatcaaaa atataattgc
     5761 tttttaatat aaaataaaca atcccactaa agattaatcc aaatatccat aggaaaatct
     5821 ttttagtgtc tttaaggatc atagagaaat atattataaa tatgttttta aagatattta
     5881 attaaaaaaa ttgcattttc caaaggtggg agaagcagga ttcgaacctg cgacctttgg
     5941 tgtataagac cactgctctg accaggctga gctatactcc cactagtaaa ttcgaaaatt
     6001 aaatggtcta ttaagtaata taattgtaga aatatattta aaggttattt acttaaactt
     6061 gagatctgaa attctctaag tctacatcta tttgctgatc agtttcctca aactctccaa
     6121 atctaccaat atttagtctc atagattttt gaaactcaga agaaaatagg tttctaaaga
     6181 tataggtttt tccaatctct attttagaaa tcgcttcatc ccacaccgtt aggtagattg
     6241 ttccagtatc gtcaccaatc aagacttcac aaactgtgtg ttttgtttcg tcaagtttag
     6301 atgtaacttc tcttatattt gttttctcta taacctttgc tatcagatct actttttttt
     6361 gaaagtgttt tatatctcgt attttttcta aaatcatgtt gtttccaagg ttatggtttt
     6421 attatatatc ttagtttaga agttatttaa aaaccttgtg tttataaaaa aatagaaaaa
     6481 gaaaagaaga tatttacata tctcccatgt ccattccgcc gggcataccc attggtgggc
     6541 ctcttggctt attggaactt cctgcaatta tgtcgtctat tcttagaatc atctcagcaa
     6601 cttctgcagc agaagtaacc gcttgtgtct taactgctat tggctctatt acctttttct
     6661 tcttcatgtc tgctaaaact ccattaacaa catctatgcc taaagctttg ttgcttgaat
     6721 cttggtgctt gcttctcaaa gacacaatag tatcaatggc atcaagacct gcgctttctg
     6781 ctaaagtctt tggtattatt tctaagactt ctgcaaatgc ctcaattgca agttgttctc
     6841 ttccaccgac agtctttgca aagttcttta gtcttaaagc aacttcaatc tctgaagaac
     6901 cgccggcagc tacaaattta ccagttttta tgcttgagat tactgcacct atggcatctg
     6961 ttaatgttct ttctgtttct gctaagactt gttctgatgt tcctcttaaa agtattgtaa
     7021 cgtaccctgg gtttggacaa ccctcaataa acaacatatc gtcgcctgca actttttttg
     7081 aatagacgtg ctctgcggtt ccaagatctt ttattgttac atcctttact cttgaaacaa
     7141 tcttcccgcc agtggctttt gcaagtctat caatatctgt ttgtcttact ctccttattg
     7201 cagttattcc gcactttgca agataatgct gagcaagatc gtctattcct ttttggcaaa
     7261 gaacaaggct tgcaccagtt gcttgtattc tatcaaccat ggctttaaga taactttcct
     7321 cttgatcaat aaatgcttga agctgatcag gagaagtaat ttgtatcttt gcttctgttt
     7381 ctggctcttt tacttcaaga gcagaactta taagaagtat ttttgcattt ttaatgtttc
     7441 ttggcatctc tgagtgaacg atttctttat caagaacaat tccatctatt aattttgttt
     7501 cagaaagact accgccaatt ttttgctcaa tctttatctg gtctctttct atttctttgt
     7561 ttgattttga aatctttagt attccttcaa gaacaatatt accaagattt gtactatatt
     7621 ctgaagcctt accagtcatg gaggtttttg caatatctag taattctgct ttgtcattaa
     7681 ttgtaacttt ctcagaaatt tcattaaaat aatcaactgc cttttctgaa gccatcttat
     7741 atcctttaat tattattgag gggtgtatat tttcatctaa aagattttca gcttttgcta
     7801 caagaccacc cgcaataaca acagcacttg tcgttccgtc gccaacttca tcgtcttgat
     7861 tttttgcaag ttctacaagc atctttccaa ttggatgttc aattgccatt tctttaagta
     7921 ttgttgcacc gtcattactt attgtaacat tgcccatctc atcaactagc atcttatcca
     7981 tgcctcttgg accaagtgtt gatttgacgg cattacttac tgctttagca accattatat
     8041 tcattctttg agcgtcctta cctctttctc ttttagaacc ttctggtaaa aacatagtat
     8101 tttgactttc attacccata atctttcacc ttttaatttt actgatggaa ttttctttca
     8161 acatcgtcta tcaagatatc acattctgct tttatctttt ctaaattttt aattattctt
     8221 attttttcaa gctctattga ttgaatattt tcaaaagtct caacagattg tttaaacatg
     8281 ccataatcta cagaagtata ttcatagcta ttcatcttct tacaaattgt ttctacaatt
     8341 ctaccaagaa aaagattcat ttctttcttt actatgcctt ttatttgttt atgcttgtct
     8401 agattctttc tcattaatct aacaactgtt gcatttggaa aaggcaattt attatcatca
     8461 agttctatgc cttcatcttc aacagcatct ataatttctt cttcaacagg ttgtattact
     8521 tggttttcta aatcattttc gctcattttt tgacccctct tttttgttat aaaaatagtt
     8581 tttaatgtat aatatatata ggtatagaag cctttaaata ggtatttttt agtataaaaa
     8641 atactaaatt tagaaataga aattaaagga atagactatc tattcctcgt cctcaaaatc
     8701 atcatcttct tcgtcaaagt cttcatcttc ttcttctgat tcgtcttctt cgtcaaagtc
     8761 ttcgaagtct tcatcttctt tttcttcgcc atcatccatc ttaaagactt ggccttctaa
     8821 gtcgttgttt attacataca ttttttattt tcaccttttg tgttttttaa aaaattattt
     8881 aatcgtcgct aaaatcgtga tctgaatcaa actcttcttc gtcttcgtct tcaaagtcct
     8941 ctggagtata ttctgtctct atgtcttcct tttcttcttt tatactctca tctccatcat
     9001 aatcaaagtc atcaagatca tagtcttttt cttcatcttt tgctgccata tatacataca
     9061 tatttatcgt tctttaaaaa aagttattat ataaaaatta tggtggcatt aactttatat
     9121 atgttatcgg taggccacaa aatgccttac tttaaatgta tttcttaact aaaatatcta
     9181 tgggagaagt atatttgcca aaggaattta tagaaaaata taaatggcta cttggagatc
     9241 aagaagcgct tgtttttttt gattcctgta aaactagact tacaaaatct ttaatcatta
     9301 atactcttaa aaccaaagat gcttcttttc ttaaagaaca aggaattgag ctagaaaaag
     9361 taaaatatat agataacgct tattttgtta aatctcttgt ttctggtctt ggtaacacca
     9421 tagaacaaaa tattggctta ttccaaatgc aagaaatctc ttccatgctt cctgcttttg
     9481 ttcttgaacc aaaagaaaca gacattgttc ttgacatgac agcggctcct ggtaacaaga
     9541 caggcatctt agcacaaata atgaataata aaggaatgat tgtggctaat gatgtggata
     9601 aaactagaat taaaacttta aaatttacat taaaaaggct aggtgtaaaa aacactataa
     9661 taacttgcag agatggaaca aaaataaact ttaattttaa atttgataaa atacttcttg
     9721 atgcgccatg ctcctgtgaa ggaatgataa gaaaaaaacc tgattcctta aagacctggt
     9781 cacaccgaga agtaaactct aaatctgaat tgcaaaaaaa gctaatagat aatgcctttt
     9841 ctttgcttaa gccaaatggt attcttgtat attctacttg tacattatct cctgaagaaa
     9901 atgaagaagt aattgatttt attttaaaga aaaataaaaa tgcaaagatt caagaaatta
     9961 gaattactgg gcttaactat tctgtgggct taacaaaata caagcacaaa aattttgatg
    10021 aagaattaaa aaaagcaata aggatttggc cacacaaaag cgaaattgaa ggctttttta
    10081 tttgtaagat tagaaaggaa taattatgac aaaaatacta gacgaaaaaa caaggaaaaa
    10141 acttataaat tatctaaatt cgcgttttgg tattgatggg tctttttttg aagattttct
    10201 ttttataatt tctggaaaat ctgtatcgat tatttataaa acaagagaaa cgcaaaagat
    10261 tattgaaaat caactaagac agaactttac tatttctttt ggtatagaaa tattctctaa
    10321 ttataaagac tatagaccgt cttctcttgg cttttcaagc tttgaaaaga aacaaataaa
    10381 gcaaaacttt gtagaactga caagaatcca agcaaacact tacttttcag gaaaagatat
    10441 tgctattgaa gacctaaaaa acaagaattt gctatcttct ggctttgttg cttgtatttc
    10501 tgaaaaccat attattggca cagcaagctt tgataaagaa aaacaaatta ttataccaaa
    10561 tctttctttt gttaatgaaa acacgaagta gttatatttc tcttgaaaga aattctttta
    10621 cttttttaaa taccgtttca tactctgtat caagcataac ttggtgccca gactcttcta
    10681 aaagaaatct ttcaacattg tttgaagaaa cagatagcat tatttctttg gagttgctta
    10741 ctggtataag atagtccttt ttactgtgaa ttataagtat atctgcattc actttagata
    10801 tttgtttttt aacaagacca actaattgaa atatctgatg aatagcattt gttgatattt
    10861 ctttgtaagt gtcttcatat tctagtaatt cattgtttag cttaataaaa tcttttcttc
    10921 ttttgctata tatgttatta ataatcaatc ttattttttc tgctgttgat tgcttggttt
    10981 gtgattgctt tttagatatc tcattaattt cagaaattat tgttgacagc tttatgtttg
    11041 ttcttttcca gtctgctaaa atgagtttac caaagctagg atctataaaa ttaatcggcg
    11101 cagacattgt aataagctta tatggccttt tctttataga gagatacaag catattgttc
    11161 cacccattga aaaacctaac aatacaactt tagaatattc ttttaataat tcttcatata
    11221 ttttctcaga aaaagaaagc caggtcgtgt atttttgttt actaaaatct gttatgtttg
    11281 tcttgtgccc tggaagaatt ggtgcgtaaa tatcataatc gtcttttaga aactctgcaa
    11341 ggggcttttg ctctattgga gatgctgcaa aaccatgtat tattaatatt gctatttctt
    11401 ttttcttttg cgctctataa taatatcctt tatttagtag ctgctctagg tctttaatca
    11461 taattttcat cttttcataa aaaaaaatat gggagaggca ggattcgaac ctgcgaccgc
    11521 ccgattatca gtcgggtgct ccaaccaggc taagctacac tcccatttta gcccgaaaga
    11581 gattcgaact cctgtcgcaa ggttcaaagc cttgcatgct tggcctctac accatcgggc
    11641 taaaaaacgg tctattaata tatattttat gaacagttat ttttataaat agaattaaaa
    11701 ataaaaatat acaatttgct atttagactt aattatatca attaattcct tatagaataa
    11761 taaaaacttt ggataaagaa caagctttgg tataaaactg ctaggataat ctatgtttcc
    11821 gttattattt aaaaagctct ctattcctga gtcttttagt tttaaaagaa gattatcaaa
    11881 ttctaaattt gttttttggt ttttaaattt gtaaagtcta taatgaatat ttaattctct
    11941 aattaatgat tttaagtgtt tattataatt ttcaaccttt ttaaaattct ttaatctttc
    12001 agttattgct tttgcagcat attctgcact ggtaagtcca aagattatgc ctccgccgct
    12061 tgttgcctta acaaagcacg ccgcatctcc tattaataat ttattttttt caaaataact
    12121 attaattggt tttgcaattg gtatttgccc tgagcaatca aatttaatat ttgttaattt
    12181 tatgtttctt tttgcaataa aatcatctag tgcttgcttt ggattttgac ctaagggcac
    12241 accaattcca attctggcaa ttgtatagct ttctgggact atccaagcaa agaaattttt
    12301 agaaaaatct ccaaaatata aattgaccat gtcttttgta aagtctccat tagctatgat
    12361 ttgataggtc gatacaaatt ctgcagttat gggtttttct gtaaatgcac ttctgactat
    12421 tgaatttggc ccatctgcgc ctattaaata tgtgtaatcc aaatagatta atcggttatc
    12481 atcaataata tttactgcaa ctgacccctc ttgaacacta actacttgat gaccaagtaa
    12541 tatctctaca ccaacatttc ttgcaagctt ttcaatttct ttatcaaaaa ttattctgtc
    12601 tataactact gtttgaaatc tttttgttct aaattgtagc tctgctttgt ttggaccata
    12661 tatatttgcc ccccttatcc tgtttactac taatttatca atatctagaa atggcgttaa
    12721 ttttaacttc ttcaaaccga aaatagaaat atgaccagta catgcttttg gaatacctat
    12781 ttctttgtca ttttcaatta tggttactaa aatatttgga ttaaatcttt ttatcaagta
    12841 accgcaatat aatccggcgg gtccgcctcc aacaataaca actcttgttt ctaaaccttt
    12901 aatcattatt tatctttttt taatagcaga aagaatatat tgataatctt cattaaattt
    12961 atctgattct gaacttttct ttgttttttc ttctgcagtt tgtgttttgt tttctgtgtt
    13021 ttctgttttt gagggctctt gtgggccatt ggctttttgt ttttcttttc cgtcctttcc
    13081 aaagtaatca tacatcatct ttttagaatc tggtcttata tctatctttt tatttttata
    13141 attatttatt gcaccaataa ttaatataat aattataatt actaaaattg tggcaattaa
    13201 atatatgata atctggatat taattggttt ctttatatct agttgctccg aagaaacgtt
    13261 gccatcaata attaatggga gctttgtatt aaattctttt gttataacat tatattttgt
    13321 agctacgtca accgcagtaa ctggtattag agatgtataa ataagctctt cactaccgtt
    13381 tggcgatatt gcggtgttgt attttattat tggatcttta acaagtattt ctacttcttt
    13441 attaaatatt aaattcgaaa cattgcttgc aaatgatttg gggatgtttt caacaattgt
    13501 tatttcttta gaaattgtgc cgatattttt aataattaat tttacagaaa agaaattttg
    13561 tgttccgatt tttaaagcgt caaagtttct atccactttg atagtattgc tgtcgtaaag
    13621 cgcttttgtt tttactaaaa tgttttgatc aagtatttcc tctaaagaca caggtacatt
    13681 tgcatcataa attacagata acgtcgtgga ttttattatt tctatttctg gtaataaatc
    13741 atctattgct tgaatttcat ttaaagaaaa ctggtaatct gcttttgctg aaagataatt
    13801 ttggtcagaa tatttatcat ttccttctga acgttttgta aatattatcg ttttcttatc
    13861 atttattgtt tgttcaagtt ttgaatctat aatttttatt gctttaaata aatcaatttc
    13921 ttcttctagt gtacttaatt tatttgcaat tttatctctc aaaagatcgc aggtcacttt
    13981 atcttcatca ataagatcaa caacgtaatt aaatgttttt gtttttttgt ttagtgcttt
    14041 atcataaaca tgaatataca ttgtgtggtt accatctggc gtttcactta aatcatattc
    14101 gctgaaatta taatccgtta cttgctgtct ggaataaata acatttaaat catctactga
    14161 atattctact ttaaaaatat cctctggcag attatatagc cctattatat aggtaacgga
    14221 gtctacaagt tcgtgcgtaa cattaaaatc tggagtcgtg ctatccatta taatctctaa
    14281 ttctgaatat gcggtttcat tttttgtgtt ttcgccggtt atttttatag tgttaattcc
    14341 tgaggaaaca aaagccgttg ttgcagagcc tttttttgta gtactgtcat atgttgataa
    14401 aatattttca taagtgctgc tatttgcttt ctttatgctt acttgcggat tttctgataa
    14461 tgcttcatcg cttaagaatt caattgtttt tgctcctgaa ttaacatata tttctccatt
    14521 aacatcattt gaaggattga cttctattat aaacctcgga cctatttttt ttatatttgc
    14581 tagaactgaa gaacttaatg ccgattcgtt atttcttaag tctaaagcac taactttaaa
    14641 ataaagatta atgtcttgat tagacatgaa agtataattg tttgtagttg attctttata
    14701 ttctgcagtg ttagttaaat tgcttgatgt tgaataataa attcggtatt ttactgtgtc
    14761 gggttctacg ctttctgttg attctgtcca agttagtttt gcagaaccgt ctgtttgtat
    14821 tgttgcagca aggtttgtag gaacagttgg agcagtatta tcataaatta tatctgaaga
    14881 acaaatagaa aatctatcgg ggtcatcttc gcttattact ttaacatata ttgttttctc
    14941 ttcatttgtt gttgaacagc cagttcctgt agttaaatta aaagagatat tatcatctgc
    15001 agcaattgct gttgagctcc cgttattaca acttatgcct atggcattaa catcatctga
    15061 aaattcaatc tctaattgga caactggatt atttgtaaaa tcgtctccat catttatttt
    15121 aacagtacaa ttatctggtc cagttgaaat agaacgggtg ttggcatcgc ttatgtttcc
    15181 aacattatcc acaccaacta ctccatagta tattgtgtct cctatgtctt tatcgtcggc
    15241 atctttacaa gtgtatgtgg ttgtgcttgt tgtacacact agtgtttgat tgcttatttc
    15301 tgtaaatgta gtatcgtctt tataaactct atattctttt aaaccagaac ctgcgccatc
    15361 tgatgacgct ccagattccc aagtaatagt aacgttctta tcataatctt ctattgataa
    15421 ttcatttttt tgggggattg atggtggtgt taaatccaca atgatattaa aatcatcttg
    15481 atagacattg ccaacattgt ctgttgcaga gatttctaca ttatatcttt cacttgtaat
    15541 tacttggtga ccagctaatg ctgcagatat ttcctcctca gatattaaaa aaccagaact
    15601 ataactagaa tctgttcttg atccaccgtt tggtaatttt acggtcattg tcgactcatt
    15661 agcaatgcca gatgcatcac ttaatataac tgctaaatca aaaacatttt tgtctgtata
    15721 aaccgttcct gttgaaacat tgccaacact tgaatctttt attattacat cagaaattgt
    15781 tgcttcagta ttatcaaaat aaacatttac ttttgtgttt ttgacagcgt cagaatctga
    15841 ataattgcct gcaatatcat ttgcatcaat aactatataa taattgccgt ctgaaattat
    15901 aggaactgct aaagaattaa tatcttttgt tctgattgcg tttgtatcca cgtattctgt
    15961 cgtatatgca gaactataat tatttaaact tgaaaccgga actagataaa caatgtgctt
    16021 atcaatatat gtttctgaat cagaaattgt gccattagaa taatcaacag ttattgctcc
    16081 agaataaaaa ttcaaatagt tagggatgcc gccgttgtag ttcacagaaa ttactgcaga
    16141 atttgtgggt tctactgcat caacagtaaa tgtagcagaa gcgccgccac ctactgcaga
    16201 tattgtaact atatcactgt caccaactgg tattgtgggt atgccctccg taattgaagc
    16261 agattgcgca gaaaaagaaa ctaccgcaga accatcagag ttctgtacat taaaatcaat
    16321 gccatcatct atatttgaag aaatatgcaa aagtgcggga taagaatcag cagtcacagt
    16381 aaaaaaaaca gactttccta tattgacata attatatcca ctattaattg taatctcact
    16441 tactgctgca aaaattgaag aatttaccaa tattaaaatc aataaaacca aaattacttt
    16501 tcttaaaaac atttatttgc accacactag cctatttttt atttatttct tgattttaat
    16561 aaataattac tataaatata atatattata tatttaaaat gtttgccttt tatatatttt
    16621 tgaaaagaat atgattaaag aactggattt taatgaaaag cacataacta tcgttggtac
    16681 tgctcacatt tctgcaaaga atagagacca agttaaagat ataatattag agaaaaaacc
    16741 aacagctgtt tgcgtagaac ttgatgctaa cagactctat gcactaatgc accctgaaga
    16801 acaaaaaaaa ccaaaatttt caaatatatt taaaactaaa cataagtctg tttttcttct
    16861 aagctacata cttggaggac tacagaaaaa tattgcaaaa aattttaata ttactcctgg
    16921 agaagaaatg ctttcagcaa tctctggagc caaagaaatt ggtgcaagaa tctacctggt
    16981 agatagagac attaatatca cattacaaaa gctcttgaaa agacttacat ttggagaaaa
    17041 aatgaaagtc ttgtttggag gattgtttat tccaaaaaaa gaacttaaaa aaatagacat
    17101 aaacaaacta atgtcagaag ttgaaacaga agaagaaaaa tcagaaataa ttgaagaaat
    17161 tatgaaactg tttacaaaaa gacataaaaa acttaaagaa atactaattg acgaaagaga
    17221 cgagtttatt gcttataatc ttcaaaagat tccagaagaa aatattatag ttgttgttgg
    17281 agccggtcat atgaacggaa ttattaataa tctaaataaa gaaatagata ttaaaaaaat
    17341 actaactatt tgaaattttt atccgttatt atctttagat aagcaccaag tgtgtgaatc
    17401 cttggaacca tgcctacgag ttctccatta actacaactc taccaagctt tatttctttt
    17461 aataattcta attctgtttg tgcttttgct attagatagc cgttacctat ctcataaggc
    17521 atgtgcgcat cagaaccggc ggtttgtatt aggccatgct tatgtgcaaa ctcttttgct
    17581 atctcgtttg ctctgcttgt taaaactcta gcattaaatg tttccattgc tggaacaaat
    17641 cttgaaattg gcccattatt cattagataa tcaaatcctc taaaatatct ccctgtatca
    17701 aaaggatggg gaaaggaaag aagtgcagat tgcgatcttg catcatctac gacttctaat
    17761 atctctgtag aggttatttt gtcgtaagta aacaatgcta aaatatgtcc taactttttt
    17821 ccattaaaag taacatctat ttcttgtcca ggaacaaaaa caatattatt ttgttttgct
    17881 aatcttgcaa gctctggcca agcccttatg gtgttatgat cagttattgc aaagccttta
    17941 aggcccgcag cttttactct tttaataata gtttctggtt cgcttacagc atcaggtgaa
    18001 aacttagagt gtatatgtaa atcaaattta ctaaattcac taggataact aattttggtt
    18061 ttagaaaaat aagtgtgtgc atctttatgt ggctttgatt ttgctttcgc ttttttctta
    18121 acttctggtt ttgaaacctt ttttattgtt tttgctttga tcaaaattat cacctaaatt
    18181 tcttacacta aaaataataa tatcaaagat gtttttaaag gttttgattt cttaagaaat
    18241 agtttaagac aagagaaaac catactaata ttctgtttcg accttcaagc caggtaatag
    18301 catatttctc ttgcctatac cgaccccgag tttcttttca gggttaattt ttcattcagc
    18361 aatcttattc gaccccccct ggaagatacc gttttaatcc attttgctcc gtgcctagtt
    18421 ttctagaatc acttggatta tttgtattat caaattccaa aatatatgtg tgttcagcag
    18481 tatatatata ctatccttaa ttttgtgtaa tttttggaac ataaattaaa taaaagatga
    18541 gccaactttt gaggcgccta taaaaaccac tgttttgagg ccttagtgaa aggttaacta
    18601 aaataagtta cttttaactt cgataagtta aaattaaaac tagaaagaaa taagtaagat
    18661 atatataaca aggttgtaaa gaacatgcgc aaaaatattt ggccagagat tattatattt
    18721 tttataaatg ctagaaagaa agattccaag cacgaatgcg ccaattattt ctataattga
    18781 gccataagct aaatgaccta tggcaaaaaa agcactagat aaaatcacac caagccttgg
    18841 agttaaaaaa cctctaaaga accattcttc taaaaatact ctaatcaaga aaagataaac
    18901 aatagcaaga ggagaatact ttaaaatctc ttctcctaca agattaagat cagaaacttt
    18961 aaaaagaaca gaaaagaaac taagcgtgta gcttaaagca aaaagcaaaa gaaaaacaat
    19021 aattgttttt ttaaaaagct ctttaaaaga aattttcttt aaaccctgta atctaaagac
    19081 ttgagaaaaa ctatttgctc tactaaatag aaatataaaa taaaaaggta tgaaaaatag
    19141 aaaaatgtct aatataaatt cttctgcaat aaataacatg gtttatcaaa agaaaagaat
    19201 gttttattca aaaacattca ttccgcattt gccgcaagca tatctggttt tgccttcttt
    19261 tgatttatga agtcccattc ttacgcctgt tccacatttt ggacactctt tcccttttcg
    19321 gtctacagtg tctccagaaa cactatagaa tttacttact ttaatattaa ctgttggctt
    19381 atgctttgtt tttcctttct ttactccgcc gcctttcttc ttacccattt aatatcactc
    19441 ctcatctttc tttttttctt cttttttttc ttcaagatct cctgttcttt tgatcttgta
    19501 gtttagttct aattccttta atatttttgc attatcataa agctttagat aagctactgc
    19561 tgattgttta cctgattctt gagaaatctt atcaacaacc aaaagctttt catcagctcc
    19621 aagcattgct gaaacctttt ttaaaagttc ttttctgctt ggtgtttttg cgcctttaac
    19681 tttagcaatt attgcagttc tgtgcaataa ctcgtttctt tgtttttcaa tttctatatt
    19741 catattaatc tcctttttat ctgcttaatc ttaatgcata tcttcctggc gtcttaactt
    19801 ccattgcttt tgctatgtct gaattttctg aatcgattat aagggcatta cccttccaga
    19861 aagttgtaaa ggttgtattt ccacacacgg ggcattcttt aattgaggac ttgtcttcat
    19921 ctgcgatatc gtcttctaca atttttctac attttctaca tgctttgtat accataaatt
    19981 atcacttttt cttttttgat ctagatttat tatctgcttt atcttctttt ccttttcctg
    20041 ttgttttctt aacaactttc ttttcttttt tcttctctgc tttttgccat tctggttttc
    20101 caagcccaaa ctgtctcatt gttaaaccga tttttgtgtc tgcgatgctt ggctttaaac
    20161 taacattagc aattcttgca attatttcgt caccaacaac aagagtttgc ttgctttctt
    20221 taccactaaa tcctggaagc tctgcattat aggaattgaa atcttccata acctgagaaa
    20281 cgtggcaaag accttcaaat gggcccatct taatgaatgc accaaaatct gttgcttgag
    20341 atatattacc tgaaacaatc tcatgaagct ttggaagata actaacaact gaaaatttaa
    20401 ctttaaaaaa tacattaggg tctgctggaa caatctctcc aatatcgtga gaaataactt
    20461 ctgttactgc aacaataact ccaatatttt tatcaattat gccttcaaaa tcattacata
    20521 attgatcaag aattatgtct tttagctctc cagacaatct ctgtggagca atactaacta
    20581 ttccctctat tgtgtatttt ttatagccca ttttatctct cctttagttt aaaaaaacat
    20641 catgtaatct taatgttaaa actctatttc ctttcttttt tatttttgct tttaatattc
    20701 tgtcgtttgt gtatattata tatttattgc taaaatatat taataaatta tccactttct
    20761 tatatctttc aattaaattt ttatcaacag ctaaaatttc aaacttattt agttctttta
    20821 atttttctat gtaatttttt aaggttttta acttttgtgg tttatctttg taaacttttt
    20881 ctatttcttt aaaagattca tcaagcacaa aaaactttgc atttggtagc actgtgagag
    20941 caaactcaaa gatgtctctt ttaagttcat acatgtaaat gaatacattt gtatcaacta
    21001 taatatttgc tactctatcg aatttgtgca taacctgata atctccaacg tcctgaaacc
    21061 aatctactga ttgctattgt ttcatctttg tagtatactg ctacattctt tagagaaata
    21121 cttactatgt ctccctttat cttctttatt gtgccaaggt gagcagcagt tccaattatt
    21181 agaactacat tctctccaag tttaatatct tctttatttg attctagtag tctttcaatt
    21241 tttatgtatt ttagttctaa ttcttgattt attaaaggct tagaactttc ttcacatatc
    21301 aattgcccaa tcatcttatc tcctgctata tagaacgggt caagagttgt tccaacagct
    21361 attaatccac cagcatgaac ttgtttaaaa gatataccat tggaatttaa tgattctatt
    21421 gtaatttcta ttggcttgtt aataccagga tataaccacg ccttctgttt ctcagatagc
    21481 gttccttgca taattgcgcc accaagaact ccgcctttta ttttctcgac tgggtttcct
    21541 ggtttattaa tatcaaaaga tctgacaatt ctcatcttta atggagcttt tgtgtctagc
    21601 tctgggactg gaattgcagt taataatgca taaactaaag catcaatgtt agcatcaaaa
    21661 tgcgcagagg ttggtatgat tggaacatga gaatagcctt catcatctaa aaactttcta
    21721 atttttttat agttttcttg tgcttcttct tttgtcacta aatctatttt gttctgagca
    21781 acaactaggt tttttagccc atgcattttt aatgccgaga aatgttcaat agttcttggc
    21841 tgtgggcatt cttcattagc cgcaacaaca agtattgcgc catttaataa tgttgcacca
    21901 gatagcatta ctgccattaa ggtctcgtgg ccaggagcat caacaaaaga aacttttctt
    21961 acaagttctc tttctccacc acacagatga catttatttg ttacaccata agcttgaaga
    22021 ccttgacaag tcttacattt atagaaacat gcatcagcat agccaagttt tatagatatt
    22081 ccttgcttga tttcatcaga atgtttatct gtccatgttc cagttatctt tgaagttaat
    22141 gttgtttttc cgtggtcaac atgtcctacc acgccgatat taagaactga ttgtatgttt
    22201 tccatattat tcaatttaat tttttctttg ccgagtgttt cttttttcta aaaagaaaaa
    22261 ctcttgtatt ttttccattt tttttatttc actttatttt ttctttttct ttgcgcctga
    22321 gtcttcttct ttcttttgct ttggaaattt ctcaaaaaag ccagttgtgt ctcctgcagt
    22381 taatttaata ttaacttgag cagttccagc aactacaact cttccagcaa ctcttactct
    22441 tcgtctctct ccggatcttt ttgttctaaa gcctatgcct tttgttgtta gaatcttttt
    22501 agaaacttca gagtcaagct ttgtgtgcat tggaaagcct tgtttatttg cgccacctgt
    22561 tattttccct tttaaccctg gagaaatatt agataaatca acctcatcgt tgattttctt
    22621 tccaagcaaa tgaagttttg tcttttcatc tgcttctgca ttatagcttt tttttgtttt
    22681 tggatctgat aatgttaatc tcaaaattca tacacctctt ttttattgat tttgtttttt
    22741 ataaatttca ttttttcttt cttcaatcat tgtttgttct tctgggctta actcgccttc
    22801 taaagcctct agtttattaa tcatatcaag gttaagagaa atctctagaa catctccctc
    22861 cttgaagtct ttaagatacg ttgcgtctcc aattgacact gcgacttcca tatccttttg
    22921 tgcaatatta atttctttac catctgattg tatgccttcg attgttccta tcttttgatc
    22981 atttaaacta actgctcctc cagaaattaa tctgccttct attatcctta cgccaacaac
    23041 tgccggctta ctgcttctaa atataaaatt tggaagcacc ttgaatctac aagggtatat
    23101 tatttccttt aataatttat caagctcttt tttctttgtt ttgcctagcc attcttgata
    23161 gtcttcaatt aacttatata taatattatt tttaaatata gggatttgta atctagtaat
    23221 ctcatcctca aactcttgat ttattttaac attaaacaag aaaagaaccc cttgaatctt
    23281 atctttttca tttaatattt ttaattctgt taaatctttg tttgtgaatt cacctatatc
    23341 tgaacttgct atttttatac attccttttc taatagtttg tgtagggcct caagagaacc
    23401 aagagtgtct gctttaacaa acgccccttc tgagccttta ataaaacaat tatatgattt
    23461 tgtttttagt ctttctactg cttctttatc acttgcagaa attattactg cgccaggcaa
    23521 acaatcttca attcctggag ccacaacctt tattcctgaa gccgcatgaa tctctgaaat
    23581 attctcaaaa attcctttct tatcaaccgc agtcatgaaa tttggtttta gtattgcttt
    23641 tacttttgag ttttgtaaac caaatcttgt tggaaaaaca atttcatctt ttaatttaag
    23701 actgccttga tagacaatga cgtcagcagt tgaacccatt cctttaagat cgccaatttc
    23761 aagaactgcc ccgtgcatat aatcgctttc ttcaattgtt atttttttgc ctagaaattt
    23821 ctgacttaag actgataaaa acaaaacagc ttctgctaga ccccagccat taacacnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgac ttaagactga taaaaacaaa
    24001 acagcttctg ctagacccca gccattaaca caagagccag gaattattgc aatctgtttt
    24061 gtaaaatctg taattctatc aaacctttct gcttgaaagc ctttatcata tagttgtcca
    24121 acaattctgt agattaattc atcaagtttt tcttctgtgt gttttgattg tgttgaaaga
    24181 ttgtttgtga tatcttttcc ttcttgatac cagccctgaa ccaaatctac tttgttagcc
    24241 acaactaaaa aaggaacctt atacatcttt agaatattga gtgtttcaat atcctgcact
    24301 tgtaagcctt tgttgatatc cattactaaa actactaggt ctgcaagact tccgcctctc
    24361 tctcttaaat tatcaaaggc gttgtgtcct ggagtgtcta tgaatagtag tcccggaata
    24421 cttaattcaa aattatattt tttagctaag tgcccgctaa tcttttgaat tacttcaatt
    24481 gttatttctg ttgcgccaat gtgttgtgta atttttcctg cttctttatc aactatacta
    24541 gtacctttaa tcttgtctag aaaggttgtc tttcctgagt cgatatgacc catgactgtg
    24601 attattggtt gtctatatac tattgtttat cacacttttt agaaactaaa acaacttttg
    24661 gtcgaaagat taaaaagaaa cttattcttt tgcaagtttc ttttgatgtc ttgtcttttc
    24721 tagatgatag gtgtctgtcc atctagtctt tagtggttta tactttttaa ctagttggtt
    24781 tttcttacat ttgcttgaac aaaagtgact tgttgttccg tcttttttag catagattat
    24841 gcctgtgcct ttttttattt ctttaccaca aaatgtacat ttcatttaca ttcaccttta
    24901 atctagcgac ccctaatgtc ctttgcttct gattctggat tcattaagat tagtacgtcg
    24961 cctttcttta ctaggcctct tacatttctt ttcttaattc ttcctttttc atagccatct
    25021 agtattctac aaagaacttg agttgcttcg cctctagaac ctgttcttcc aacaatttca
    25081 acaatttctg ctggaactcc ttcttctggc attattgatc acctattctg ttttttcttc
    25141 agcaacttct tctttcttag ctactggttt ctcttcaacc tttggttctt ctttcttttc
    25201 aacctttggc ttttctttct tttcaacctt tggttcttct ttcttttcaa cctttggttc
    25261 ttctttctta gctactggtt ttttctctgc tggttttggt ttatcttcag cttttgattt
    25321 tccaccactt aattcttcaa tctttgagat taatgctgag aattcctttt ttaaaactcc
    25381 ttcatcaaca attgctatag cacttgcttt tgcatttatt cctgaaatct tgccaagctc
    25441 ttcttttgtt gaaacataac tgaatgctat gtttttttct ttacaaatta ttggaagatg
    25501 cattacgatt tctgcaggag taatgtcttc agcaattaat actaattttg ctgtatttct
    25561 ctctaccatt tttgttactt cgttgattcc tacttttatt ttacctgatt tagaaatctt
    25621 tgaaacaaca tcagcttgaa gattctttaa atcctctggt acgctgaact taacatagtc
    25681 tgccatttta aaacctccaa aatgttttct tcatcggctt ttttctttag aaaaaagtat
    25741 atttagtata atatatttaa gccaggaaac ctttataaat agattcatct tttcttagta
    25801 tttatgagcc taaataccat taaaaacgcg ctaattacag aagaaaagaa atcactttgt
    25861 ttaagaaaac aaattggcgc tgttgttgtt aagaatggaa aaattattgg tagtggacat
    25921 aatggtaatt ctagtaatat tcctccttgt tctgttattg gctgtgcaaa agactatttt
    25981 aagtttaggc cagggctacg taacgagcta tgtactggca tatgtgctga gcaaagagca
    26041 atgattgatg cgataaagaa tgggcacaac cttaaaggcg cagaactata ttcaacatat
    26101 agtccttgtg tttcttgtgt aagatatatg attgaatttg gaatcaaaaa ggtcttttat
    26161 aaaaacgact atagagatgc atttgccagg caaatacaaa aattagggaa aatagaaaga
    26221 gaaaagattt agaatattta tctaaacttt tctggtttat aatctgtttg tattgggcaa
    26281 ttagtaaata tttctgacca atttgtgaca ttttctacat cccatgagct tatgtcttgg
    26341 ttgaaactgg atgcattatt aaacatacct cccatattag ttacttttga gacattaaaa
    26401 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntacctgac atatcggtta
    26521 cattagttgt attaaaatct aagggctgat taaaactggt tgcatcagaa aacatacctg
    26581 acatatcggt tacattagtt gtattaaaat ctaagggctg attaaaactt gatgcattcc
    26641 taaacatctc agacatatca gttacattag aggtatcaaa atttgataaa agtcggttga
    26701 aattggaagc atctctaaac atccaatgca tattggttac attagaagta tcaaaagaag
    26761 ataaagactg attgaaacta cgtgcacctg aaaacatacc atacatatca gttacattag
    26821 aagtatcaaa agaagataaa gactgattga aactatgtgc atcaaaaaac atagcgccca
    26881 tattagttac attagaagta tcaaaagaag ataaagactg attgaaacta ctcgcacctg
    26941 aaaacatact acgcatatta gttacattag aagtatcaaa agaagataaa ggctggttga
    27001 aactactcgc acctgaaaac ataccccaca tatcagttac attagaagta tcaaaagaag
    27061 ataatggttg attgaaacta gatgcattac gaaacatatg ttgcatacca gttacattag
    27121 aggtatcgaa attagataaa gactggttga aactagaagc attacgaaac atataagaca
    27181 tattgcctac tttagaggtg tcaaaatttg atatgggctg attgaaactc atatcataaa
    27241 tacatccaaa catattacgc atattaatta cattagaggt atcaaaagaa gataatggct
    27301 ggttaaaatc gatcgcacac tgaaacatcg caaacatatc tgttacacta gaggtattaa
    27361 aatctaaagg ttgattgaaa ctagaagcat tagaaaacat aaaagacata ttaattacat
    27421 tagatgtatc aaaagacaat ggttggttaa aactagaggc catattaaac atagacccca
    27481 tattagttac tttagaggta tcccaagaat ttatgctttc attaaatact aaactactac
    27541 atttataaaa tatgccttct aaatttgttg tgccatttaa atccagtacg tctgtagcag
    27601 taatatctag attagaacag ccataaaaat aataaccatt gttacctaga tttaggttac
    27661 cccagttttt tatttctata agttttaatc tgtcgccatt attattaaaa cgaaatcctt
    27721 taattactcc agttattttt atttcataaa ttcctggctc tgaataagta tgtgttgctt
    27781 ctggctgatt ccaagaagta atatgactat ttgtaccatc attccaatca acattaaaat
    27841 tataagttcc tgatgattct aatggcaacc tgatttgact gctatcagaa gaagggagat
    27901 taccagcatc tgaattggta ggggttgctt ttggctggtt caaagaagtg atattactat
    27961 ctgtgtcatc attccaatca acactaaaat tataagtatc tattccttct aatggtgatc
    28021 catcttctaa tgttgatccg atttgattgc cagtagaaga aggagagggt gacccagaag
    28081 aaccatcact tgtatttctt gtatcaaata ctaatattgt tggagggcca gtttcttctg
    28141 aacctggtgg ctcatcccca tcttcatcat cgctatctcc atcatctgga acaatgtggc
    28201 tactaccacc atccattgat acttgatcaa caccatcata aattattgct ttatcataac
    28261 tggtttttaa ttcagattca ttcatgtctc tagaaattcc cgctgaacct cttgatctct
    28321 caccaatatt tccaaaataa aaagttccaa atattattac tccaacaact aaaacaccca
    28381 ttataatcaa aacttccatt gttgcttgtg cttttctttt taaatcagtt ttttgtggtt
    28441 tgttgttttt agataaagtt tctctatttc taattaattt ttgacccatt atttctcaac
    28501 ccaataatct aatataaata tactataata gaataagtaa caaaaatata taaacttttg
    28561 gtttttggtt ataaaaaaat agacaatgat aattttttaa aatttaaaaa aataaaaata
    28621 gaaaaaaact acttcttcca ataatcgtca aaatgagtaa gaagtgcttt tgtaagactt
    28681 ggattgtttt ttaaaattgt taaatgatat gtctcttttg gtgaagataa atcatttaaa
    28741 gacataacaa ctttgttctt atcaataata aaagcatcca ttgagtgctc tttttcttgt
    28801 atactaaaat tctttggaag atcaaagtct tttgttatag gtttagagat taatttaaca
    28861 ttaatttctg gcatctctga aactggctta aatgcatcaa ctctatcatc aatatttgta
    28921 aaaccagtaa tttctttttt tgcgccttca aattcttgag ataataactt attatagtct
    28981 ttaatattct taacttgtaa aatcttttct tgaactccag aaacctggtt tgcccacgtc
    29041 tctttactta agacgtcttt tacttgtttg tcaagaactg acaattctct ttgtttatta
    29101 tctattaagt atttaaaaat ctcattagga tcaattacct tgtatttttt tggtctacca
    29161 tagacctcta gaactagtcc tttttctttt aatttttcta aaacatcata gattctagtc
    29221 tttggaatct ctgcgtttct actgatgtgt ggagcatctg cttcaacaaa attaaataaa
    29281 gtagttaatg ttctggcctc atattccgta agaccaaagt ctaataacaa actcaatatt
    29341 ttactgtttt gcatagaacc cctattcaca ttaatttatt atattatata tattaacgat
    29401 agttaaataa acctattctt ttgaaacttc tgctggcttt tcttcttttt tctcgttttc
    29461 tactgtcgga gcggcagttg ttgctgctgg ggctgccgct gttgctattg gcatggcagc
    29521 tactaaaggc gttcttctaa catatggtct tcttggtgcg ctaccggttg tgctacttgg
    29581 tcttctacca ccagctgctg gctttcttgc agtagcttta gttgaaacct ttctaactct
    29641 ctttcctgga agtggctgat cctcatccat ttctttttcg ccattttcgc tattatatac
    29701 taaatcgtca attgaatatg catagtcttt gtctgatttt attattccgt ttcttaactc
    29761 aagttctctt attaataacc agaagaacaa tgctactgat tttcgcccct tattgttata
    29821 aggaacaatc aagtctatgt ttcttgtgtt gcttgaagtt gtacaaagag caacaattgg
    29881 gatatttgct tttgtgcttt caacaactgc ttgtctatca acattagggt ctgcaataaa
    29941 tactaatttt ggctctataa agtgttctga ttcaggattt gtaaaggttc ctggaataaa
    30001 tctgtttaat tttgatttat aaccaaatgc ttgctctaaa attttaacac cgggaagagc
    30061 atatttcttt ctagagataa acacaacatc tgtaggttca tatttagaaa taaaatcaat
    30121 tgcaatcttt attcttttat ctattatttg aacatccatg acattgatct tatcgtttct
    30181 agacttgaat acaaattttc tcattggtcc attcttgtat ttggttccaa tgtgtattcc
    30241 attctttaga tataaatcaa cattaacaag agtcctactc tgtgatggtg ttctgttttc
    30301 gttttcaaaa tttcttggtt ttgcatttac tttatccatt tttatcacat ttaattatac
    30361 ttcataatct catcaataac ttcaacatga gttaaaagca ttcttctaca gcaatattta
    30421 tctatgccta agatatttaa tgcttcctcc ggagtcttct tctcctgagt tatcaatctt
    30481 acatactcat catatttgtc agctacaacc tgaccacacg aaaaacatct tactggacaa
    30541 atcattattt caccttttag aatgttgctt tttctttctt gcacctttac ctaattggtg
    30601 ctttggttct tttcttctaa catcatcaac aagaagatgc ctatcatatg tcataatctt
    30661 atcttttact ttttctctat tgtttgctgc aagtattgat ttagcaatac atgatcttgt
    30721 tgcttgaact tgcgacatta cgccgccacc agaaacatta acaaaataat caagcttatt
    30781 tgcttcttcg tcaagaagaa tcattggctc ttttattagc ccttgcttgt accctgaata
    30841 atatgcctct agagaatggc cattaacacg aactttgcct gttcctgctt ttgacataca
    30901 tcttgcaatt gcattttttc ttgaagcttt aacaaaataa ttctttacca tttttttcac
    30961 ctaataatta tacttttaat tttgcaccta atagtacgca aatctcattt agattcatgc
    31021 cttttaattt tggtttttct acaacttctt ttgacatgtc tttaacatat gcttctggag
    31081 catcaacaaa cactttaaga ttttttaaag cagcttgacc tcttgcattt cttggaagca
    31141 ttcctcttac tgcaaacttt atcatcttgt ctggatatct tggatatttc gcattcttta
    31201 actgattacc ttttcctttt ctgtgtgttc tttctttaaa tcttgccatg atgtcttctt
    31261 ttgttccaga aataaacgcg tcgttagcat taacaactat tattttttct gagcttctta
    31321 aaagctttgg agcaatctca gaagcaagtc ttccaagtat tttatttttt gcatttaata
    31381 ctatcatatg ttttcaccaa aataaatttt attttattaa ttttactttt ttataatcta
    31441 tattgtcctt acagaaatct cttaatgtct ttagtccttt atattttgtt tttgcagatt
    31501 ctgaaaagct taaagcatag attgttatct ttttttctaa aatccctgtt ccaagtattt
    31561 tcccaggaat aactacaatg tctccgtcgt taatataaga catcttctgt aaattaaaaa
    31621 gattctttga cggttgtttt tttgaagatg cttctaaaat ctttgcaacg ctaacatata
    31681 tttctttttt cttatccttt gataacttat atagattctc aatcaacttt tgagtttgta
    31741 aattctttgt atcttttttc ataatcattc tcttttttaa tttttttaaa tattaatctt
    31801 ttttttagtt cgtagttttt tctttgggcc tgaaagaact ttctttttta caaaaaacac
    31861 aagaaaacta atattttctg tgcccagaaa aatttttgat ttttcttggg gaaagggcct
    31921 ctgctgaggg caggattcga acctgcgaag cactaagcac tggatcttaa gtccagcccc
    31981 tttgaccgct cgggaacccc agcgctaagt ttagtcctct tacattactt aaatttcttt
    32041 ttaagtaaca tcatatactc tttagtatat ctatcaagca actttacaat tgcttcaatt
    32101 ggtaattggc caattaattc tatgtttaat atataattat tagtatcgtt aagttttaaa
    32161 caactctttg gacaagcatc aacacatgct tggcagtttg tacatttatt tacgtcatca
    32221 atctttggct ttcctgaaac catcttcata cattttactg gacaagcatt aacacaagcc
    32281 tcacaaccat cacatcctct taatatctca agttctggca actgcttaaa agaaacaatt
    32341 gcaggagaga acttaacgtg tgttcttcct tgaccttgaa tagcataagc atcaagtctt
    32401 agtctttggt tttcttttag ttttgttaat ggtatggtgt catatactgg cttaacgtcc
    32461 tcgtcatttg attgtaaatc gccagaataa acggtgcatg ggccgtcttt ctctaaagtt
    32521 agagttactt tctttcctgt tgattctgtt ggtgttgtta atggtactaa tgcaatccta
    32581 ttagcaagca tctcatcaac aataactgaa tcgttctcat aaaaatcaat ctcatcaact
    32641 gcatagactg ataaacaata acttatcttc tttcttaaag tattcatgtc aatcgggtca
    32701 aagccctctg caagatactt gtaagttttt ccaacagttt caagataagt tatttttgcc
    32761 atattgttct gcctaccatt ttattttttc tttctaaatt tcttttgttt tctacagccg
    32821 tcgtgtggga ctggcgtagt gtttgagata cttaaaatat ttaagcctgc tcttgttagt
    32881 gtctttaaag aagactcagc gccttgccca ggattataat aaagattacc acctggtgct
    32941 ctaatcttaa catgaacatt ctttatgcct ttatcatttg ctttttcagc aacaacttga
    33001 gcaatcttag ttgcaatata tggagagccc tctttatgtt gagactttgt catctggccg
    33061 cctgagcatt ttgcaataac ttctgaacca gtctcgtctg ttagaacaat tatagtgttg
    33121 tttcttgaag caaagatatt aacaatacct gtttttccta tgcctgccat aatcattcac
    33181 tctcctcttt tttctcttct ttattttgct ttactggttt aacaaaaggc tttccttttt
    33241 gttcttgtgc tctatcgatt gctgctttta ttttagtatc aatgtaagct attgaatctc
    33301 ctgccataac aagagaacta ggtgctgttc tttttgtgcc gtttactatt atgtggccat
    33361 gtgttattaa ttgtcttgct tgttttggcg ttaatgcaag acctcttcta aagacttgtg
    33421 tttgtagtct tctttcaagt aaatctgaag cctgtaattt taaaacatca tcgattgtat
    33481 gatcctttgt aaagatgccg gttctttgta atttccctat taactcgcta tttcttttct
    33541 cgtagttatc aacattacct gctagaagtt ttcttgccat ctttctatag tttctaacaa
    33601 ctgattctgc tttccagatt tctctttttg tttttaaacc atattctctt tcaagctttc
    33661 tatcgataat cattttttgt ttatcgtgaa gctttcttgg aacatcatac tgtctgttta
    33721 atcttcttgg ttctcccatt atttttcacc tttatttttt tactgtgcca ctactttttc
    33781 ttttctacac caattgttga atgtcttcta ccagttgatc ttgctttttg gccccttact
    33841 cttagacccc actgtaatct taaacctcta tagcttttgg atttaccaag tctttgaagt
    33901 tcttttctct caactagctt tagatctgcc atgatgtatt gagcttgggt accatctctt
    33961 tgttttggtc tattaagtaa ccaagcaggc actctcttgt ctaagtctgc tattattttt
    34021 tctatagctt caacatcttt ttctgttaaa tttcctactc tattaataac tttaccagaa
    34081 tccttgtaaa atatttcttc tattactttt cctagtcttt tacttatgcc atagattaat
    34141 gttaaaccat cagagattgg catatatcct ggaatatctc tgtttgcaat tcttactatg
    34201 tgcctgaagt ctgctccttc tttagcagga ttaaaatggc ttggctttgg cttaggttta
    34261 accattttca atttatctgc agaaatatga gttgatcctt ctgacctact tttcttaact
    34321 tttagttctg gtgtattatt aattttttta tcttttgcca ttacatatca catttaatta
    34381 ttctaaagga aatacatgta gtattcctat cttttgctct ttgtctttta tcttatagta
    34441 gatatatttg gtatccatct tgtcaataac aatctgctta cctatatttc taccttgcat
    34501 tattatgcat tttcttcctt tctctaataa acccataaat catcactcta ttttttttaa
    34561 tatcattttt atatatttta tatatcttaa atcaacatct tcttctgttt taatattgtg
    34621 ctttaacatt actgcatcca aatatgcaat ctgcctacac tttgggcaaa gaacacttga
    34681 taatagattt tcgggccttc tctcagtctt acttagtctt ttaacttcta ctgttctttt
    34741 gccatgcggc ataccgtgaa gaattgcttt acaaactgca cacttgtgtt ttgaagtttt
    34801 tttctttaca agtactctgg attttttacc actaggagta attgtagttt tatgtttttt
    34861 aaatttctga tttggattag gcatattgtt ttttcaccat attatttttt cttatagtct
    34921 gcaggaacat agtccttata tttttgttca aatattttat cgtaaatatt atttgcgatt
    34981 attgaaaata taaaagtcat aatgaaatat gtccaaaaac catgaagatc aaatgtccat
    35041 ggaaatgttg tgtctatgaa ttttattgca ccataaaatt tatttataac atacaaaaaa
    35101 ggaatcgcca taatcattaa tggaatcatg gttttaaaca ttcctttaaa ttgtctaccc
    35161 ataaggctga atgcttcttt ttgtttttct tggactgcaa tgtaattgtt ttctttcatt
    35221 aatactttca tttctgcttg aatttcttta tttcttcttt ttacaaagaa atattctttt
    35281 ggatcagaaa agaaatgctg tattgctctg tttaatatta caaatatcaa tgctaaaagt
    35341 atcaaatcta tccaatgtga tccggttatt ggtatgttca tatattatca cttatttaac
    35401 attgttttca aaaaattacc agactcgaac aactgatctt ttgctaattg ttcatagaat
    35461 ctataaacta ttccaactgt taacaaaatt ccggttcctg ttcctattgc tccggtgatg
    35521 tctgcaaaca ctgcgaaaag cccaacagta attgaaccca taatcacaat tgttggaata
    35581 tatctgttaa gtactgttac taaaactctt tcatctcttc taaatccagg tattgacatc
    35641 cctgatctct gtaactgacc tgcaacattc tctgtacctt ggccaccaac ttcaacccag
    35701 aacttaccaa agattacaca cattaaaaca aataatattg aatatcctaa aactgaaagc
    35761 accgtgcccc aagttgcacc accaaagatt gcagtctgca ataacccttg tgacggattt
    35821 aaaaaatttg caactggtgt taaaaaatta ccaagcgctt tgcctttcgc aagagatgca
    35881 aataaagcaa tatttgcaaa caacgttcct gtaagaatta caggcatgtt tgaaacatac
    35941 attaatttaa ctggaaaatt tgcacccatt ccacttctac caactgtcaa tggaatatta
    36001 acatgtatgc tttcgacata aacaactgca aagaaaacca ataatgtaaa taatattggc
    36061 aataatactg ttgctaataa ctcagtcatt gcgccagttg ctgcatagct aattccttgg
    36121 aataataatc ctccaggata actaattgca gagtttggga aactaaatat ttgccaaatg
    36181 attgcttggc aaacacctgc agcaatgaat agtccaattc ctgaacctat tccatatttg
    36241 cttacaactt catctagata aattactatt attgcaccaa ttactatttg taatattacg
    36301 attaaaagca ttccttgtgc tggaacaagc ataccaaatg ctgaataaat tattgcttca
    36361 actattgcaa gaacaattgc aagaagtttt tgtgccccag agaattttgc tctgtcttct
    36421 gactttgaaa aatcaaaatt aataattttg actcctgcaa gcaattgcaa gataattgat
    36481 gctaaaacaa ttgggccaat accaagagta attaatgtac caatatcact tgcaagtatc
    36541 atctgataca tgctgatatt tgcggtttga ctaggatcaa gaccaaacaa agttattgtt
    36601 cctaatataa agtaaataac aagaacaact aatgtccaaa gcaacttttg cctgacattt
    36661 ggaactttac ttggttcctt gactgaaggc agtgccctca atattccatc aaaaaaactc
    36721 attgaacctt tcccccggct ttttcaatct tttctttagc aaccgctgtt actaaagcat
    36781 tattaaaaac aactggcttt gtgatatttc ctttaccaag aatcttatca tagcctagtt
    36841 cttttaagtt tatttctttc ttgtctttaa tcatatcaga caatgcctca agagtaattg
    36901 tctttaactt tgtctttggt tttaagaaaa ttctttgacc aaaatcaaca tagtattttg
    36961 tgaacttgtg ctttgttgaa ccagctctgc ctttaccgcc agtacaacca gaacctctag
    37021 cgttctttgt attaccgtga ccgtgagttc tctttcctct taatctgtca atttttcttt
    37081 ttaccataaa ttttcacaaa tttaaatcat ttttaataga agagtattta tttggtcagc
    37141 tctataacca agtactccgc cttccttaaa tgtcttttta attccttttc tatcaaagcc
    37201 ttttgttggt ggcttaagtc tgaaaacctt cttgatacca agatcttcaa gctttgcttt
    37261 tcctgagact atttcctcaa taactttgtc ttgttttttt tcatccttgt aatgttcttt
    37321 aaattttatt aatttgcctt tctcattata gcaatagcct cttttgttta gcattttctt
    37381 taatgcctct tttgatattt caccataggt taccatgttt cttaacttat gaagcattcc
    37441 tttgacatat ttgtcttcag gatatataac acaatgattt actcttgtta agtttaataa
    37501 cttcattgtg ttttcaatat ttacattgac ctttgctggc cctcttattc taattattgc
    37561 aaacatatct tatttcactt tttattcttt attttcttta tcttctgttt ctatttcttt
    37621 ttcttttgtc tttaaatctg aaatcttata aagtgcatca attgttgctc ttgtaaagtt
    37681 tagtttagta tctgttgcac ctcttgtgtt tgaccaaaga tcttttattc ctgcaagatc
    37741 aaatattggt ttaatggtat cgcttactgc tagaccaata ccgtttggtg caggtaaaag
    37801 ctctactctt aaagacgcgc acttgccaat gactttataa ggtattgagt gcttctttga
    37861 acaattacat tcccaagaac cgcagcctct tcttactgga acaatgttta actttgcatc
    37921 ttttattgct ttttcttgag caactattct ttcaatattt tttcctgttc caacgcctat
    37981 gtggccatta ccatctccta ttattactga gactctaaaa gagaaatgtc taccggccct
    38041 agtcactctt gttgttttct taacatcaat aactttcatt ttaagatcag aaataaaaca
    38101 atcaacaact tcgggttcca ttattttttt accaagcttg aagacatcgt caagagatgc
    38161 taattctcct gcctttactt ttttaccaag ctctgttttt ggaatccaag catctttatc
    38221 aaatactttt tcttctcttt tttctcttct tggtcttctt tctctttcgc taaatgcaca
    38281 catagaataa tcacatcttt aatatttttt ctttagttac tataaactcc ttatctattg
    38341 gtttctttaa atattctgcc ataacctttc cttcaattct atcttttcct ggaaaagcct
    38401 tttcatcatg tggacaatca agcccagaat ctactattgc ttttaagcct gcaaaaatct
    38461 tgttaccgga cttatagttt cttgcaccta aatcaagaat aacttcttta actcctttct
    38521 tctttgcttt ctgagctaaa agtaaagacg ttaagtaaat tgcaggagta ttctttgtat
    38581 ttccggaata acctaaagcc tttaattcct ttgaatctgc gcttgctaaa gtcttgtctc
    38641 catccttatc atatcttatt aattgagctt taacaagagc atttgttact cttagaacga
    38701 ctctgtcctt tttagactta agaaccgcaa gtcttttctt atagtcagtc ttttgctctc
    38761 ttcttcttct aaatgcaact ttaaatgttg gtcctgatgt catttctttt caccactact
    38821 aatatattct tctaaatgtt tcttacctct aaagtagtta ccgtttattc tttggtaaat
    38881 ttcacgatag tttttattat ttaatttgtc ttcttttctt aattctctta gtctttttct
    38941 tagagcccta atcttgtcat tatatagatt tctgctctgt cttgcttttg gtgttccttt
    39001 tctttttccc tgtcctcttt ttctttttaa ttgctttttc tttaaaagga ctcttgctct
    39061 gcctcttgat tggccagtat cgtttttctt ctttattaca ccttttgcta ttgcgtctct
    39121 aacatcttgc ttagttattg ctttatcaac aaactcttta ttgtcttgat atttcttctc
    39181 atcaataact aacttatgct tgcttttctt aaggatcttt cctgaaaatg tttttaattt
    39241 atctaacttc ataggtttac accatttttt aatttaatat tttaaaacct ttttcttttg
    39301 caacttttct taactctgtt ctttttttta agccaagtgt tccattaaat cttattgcaa
    39361 tctcttttgg gtttagtttg ttaatatctg tcacagtcct tacgatacac tcctttaaac
    39421 cacaaggatg catacctttc tctgtgttct tgtggccgta accgatcttt ggttgtggtc
    39481 ctttcttaac agacatgtgt ctatcgatac ctcttggata tctccatttt tgaacctttc
    39541 tattgtgttt ggttcttcta agtgttaaag ggaaatgccc tctaaagttt ggtgtatcat
    39601 tactcataat cattcacgct ttgctttttc aacaatataa caaccatcat caaatattct
    39661 aaaatctctt ccaacaactc ttgttgcctt ttcaatattt cctgctgttt ggcccgctgc
    39721 atatttgtcg tgacctttta ctattatttc tttccctttg acttctactt tacaacctgg
    39781 aagaattctt gtctttcttg gttttttctc tcctaagaaa tttgagatta caacttcgtg
    39841 gccttcaacc tttacagtta ttggaaagtg agagtaaaca actgaaagct tgtaaacata
    39901 gtctttttca aaacccttta aagcattaat tatatgcttt tctgtgctgt taacaacagc
    39961 tttaattgtt ttctttttac tcttaggtat aagctcgatc tctccattct caaattttgc
    40021 agaaacataa actggattaa actcaacatc tgttttcttt ccagactttt caagaatcaa
    40081 atcatgagaa ccaagatgca ctttaacatc tccaggtatc tttatctttg tttctttaat
    40141 ttcttttttt tctttttttg cttgcataat ctttttcaca atttataaat ttaataaatg
    40201 taaccaacaa ttcttccacc aagacctttt tctttagcat ctttgtggct cattatgcct
    40261 tgtgttgtgc ttacaattaa tacaccaacg tctcttgaga taagatatga cttttcaaac
    40321 ttttcatagt cttgcttttt cacaggatat cttggtttaa ctgctttaca tttgtttatt
    40381 cttccgccaa gataaacaac aacttgctta catggtttct tttctaaaac tttgtacatc
    40441 ttaatatatc cttctttttt tagaagagtt aatacacttt ctaaaaactt agaatgattt
    40501 agtgtgcaag tttgtttaga agccatctct gcattattta tgttgtttaa tgcgtttgat
    40561 attggatcgt ttaagctcat caattttcac ctaataaata tttaatctaa ttttttaaat
    40621 cccaacttgt ctgcataatc tttaaagcat cttctacaga tataaagatt atatttcctt
    40681 ataagtccct tgtttgtttc gcaaaatcta cactttctag ttcttttaag ttctttactt
    40741 gttttgccta gctttttata atctatttca ctcattcaac cactattttt aatttttctt
    40801 ttgcaaactc cattgcctcg tcttttgtga ttctgtgttt ctttccaact tttgcgatat
    40861 tgtattttct ttgctttact ctgtatcctc ttcttttcaa agacacacaa acatcaaaac
    40921 ccctgattcc aagatttggg tcgtatttga ttcctggaac atctaagtat tcttttatgc
    40981 cataagaaaa gtgcccttca tttgaaaaag actttttctt tatcttgtat tcttttgctt
    41041 ttaaggttaa atctataaag tcaagcgcct cttttcctct tagagttacc ttcccgccga
    41101 ttggtagacc aggtcttatt ccccaggttg gtaatttaac tgttgccatt gtcttaactg
    41161 gttttttctt agttaccaat tgtagaatct ttaaaccttt ttcaagctct tcaccagctt
    41221 cgccaatacc catgtttacg gttacctttt caataaagat ttccctgttt gggttgttat
    41281 ttttgtctgt agtcatgaaa tatccctttt tagttgatta caaaaacatt cttttctgta
    41341 gtttcaaact ctttatcatc aatctttaat tttactaatt ctttttttat catgttccct
    41401 ttctttactg caacaatgat tccggttttc ccaatgtgtt taccaccaat taagaaaact
    41461 tttctgcctt tctctaaagg gtaatattct tttatggtgt tgtcttcaag atcaagtttt
    41521 atagaagcct ttgtcttgta tgcttgattt gttgtaatta cacttcttcc atcgtttgtt
    41581 attaattgta tctgcccttt cttggttatc atttttttaa ttatcttaca aactttatat
    41641 tttgtttcgt cttgatttat ttctattaat gttattagtc cgttttgttt gtatattact
    41701 ttgtatgcct tctttatctt tggtaactct ataatatcaa ataatccaac tgcgtaattg
    41761 attgacttaa ttggtttttt atcaactgcc atttctttgt ttctaacaac aaaagttgat
    41821 tctctgttgt tttctgttaa atgtaatatg tctcttaaga cataacctgc actaacaacc
    41881 tttagaagag tgtgctttcc tgggtctggt cttagaacaa aggttttttt cttatcaacc
    41941 ttgtgtacct ttccgacatt ttgagttttt aaaactcttg tacttccttt ctttgtcata
    42001 ttataagtca ccaattattt ttttgatttt ttttcaacaa atcttttttt atcatttaaa
    42061 actaatgttg tgattactaa atttgaagcc ttaaatggca ataatttttc ctgacctctt
    42121 gaatttgtta ctttgaagtc ttttagataa actgtgcctt tgtcgtagtt tactttttct
    42181 acaacgcttg tctttccttt attgtcccca acaagaactt taacggtgtc tccttttctt
    42241 aaagacgctc ttttcttgtt tacttgtttt acaagctgct tgtcgattgg agcaactaat
    42301 aattttcttt taacatgata aacgccagtt aacattcttt ttctgttctt tcctggtttt
    42361 tttgttttat taatcatttt ttattcacct aaataatctt aaataactgc acttgcaaga
    42421 cccgcaatct ttgggtatct ctcaccaact tcttttgcaa cacaaccttt gatttcagaa
    42481 ccttttggaa aaccctttgg atctgttaaa acaactgcgt tgtcttcaaa gcaaactctc
    42541 cagccgtttt ttcttctata ttcttttctt actctaacaa caactgctct ttccattttc
    42601 ttcttcattg ctggctttcc ttttttaaca acaacattta atagagatgc aattcctgca
    42661 gactgtacgc cttttctttt tgttttaata ttgatgactg aaataatctc tatttcttta
    42721 gcaccactat tgtctgaaca taaacatcta gaaccaatct gaagtcctct tgttactttt
    42781 gcacttattg gtttcattct tcttcaacct cttcttcctc tataccttcg tatttgtcat
    42841 catctttaat tatgtcttcc atgtggctta cagttcctag ttttttcata ataacaaaag
    42901 actttgtttt acttagtttt cttgtttcgc ccacaaggac tatgtctcct atattaatct
    42961 ccatacattc tgggcagtgt actggaatct ttactttctt tttcatatat ctttcatatt
    43021 ttctgatata ctttgttttt tccatttgta ttactgcggt ctttatggct tttttcttaa
    43081 ccacaatacc ctgtaaagtc tttcctctta cgcttatctg tccatgaatc gggcagtttt
    43141 tatcaagaca tgttttagca tccataactt ttcacatatt tattgtttct aaatctatta
    43201 tttcattttt tttaatttca aatatttttt ctttatcatc tctaattttt agcaaatctt
    43261 ttgtttggta gataattatt cctttttttc ctatctttgt tctgtctgag gaatttttaa
    43321 taattataat ctttccaatc agctcagaat ttttgacatt aagcatcgat taattctcca
    43381 agatatcctt tctctattaa tagttttttt attctttctt tttgctcgcc ttgtagttca
    43441 ataacatttt ctttgactgt tcccccacaa gctaatttct ttttaagata cttgaaaact
    43501 tcctgttgct cgttagcatc tgtaaaccct gaaatcagag tcgcatactt cttgaatttc
    43561 tttctctctg ttctgatttt tatctgttgt tgctctcttg ttctaagatc acaagtacag
    43621 attgtttttg gtaaatgaca tacaggacaa atctcagcca tattttactt taacacctca
    43681 caaattattt cttcttatct tttgtttttg gagttacttc tttttttgaa gcaaccttaa
    43741 ctggaacttc tttttgcttt ttctttagag catttaattt tgttagtagt cttgctatct
    43801 gttttttaag agttgttttc tttgaaggat tagtggattt tgtttttgaa gctagcattc
    43861 ctttttcctt tgctagatct agttttaaat ctctaacttt attttcaatt tcaaccattg
    43921 aatttaatgc tactgctttt ttctttgtca tctaatcact cttctattat ttcttctact
    43981 atctctaatt tttcttcttt agtttctttc ttttcttttt tcactcttgg tttcttttcc
    44041 gttttctctg ttgacttttc ttcttttgct tttttctttt cttcaacttt ttctttagca
    44101 actattgttt ctttctcaac ttcttcttct ctattctcta gccttttttg ttctcttgct
    44161 agcttgtgtc tatgagataa ctctctaaca tcgatcttgt ctgaaaagac agtttctggc
    44221 tgaacaattc taagtcttat accgattgtt ccaaggcctg gaaaggttgt tcttttttca
    44281 aaagcaacaa ggtctttttg agaaccggct ttttttaagt atccaaatgc gtatcttgaa
    44341 gatctttttc tttgaccttt acctgcggtg ttaccttttg caacaatttc tgcacctact
    44401 gcgcctgcat ttttcatctg cgtcatcgct ctatagactg ctgacttcca agcaattcct
    44461 cttccaatat catttgaaag gttttcaatt attactcttg gatcaagatc tttgttttgt
    44521 agttctccaa tctctatatg cactttattt aacccaaagt ctctttcaag atcctcagtt
    44581 aatgctttaa tattaaatcc gcttttacca attacaaagc caggtcttaa agcgtagata
    44641 acaactcttg tggaaattgg cgacttaaca acctcgacat ctataaatcc agcttgcttt
    44701 agtttctttt gtagatactg agcaattgcc atctttttta gtttgtcttt gacaaaaaca
    44761 ttctccttca tgtttgatct taagcctcca ccactattac ttcaatattt gtagattttg
    44821 attctcttgt ctttccgccg attctaccca ttggctgtat cttttgtctt gttacacctt
    44881 ttgttgcaaa catgctttct acctttaact tttctgtgtc taggtttttg ttttcagcat
    44941 ttgcttttgc ttcttcaagc ataagtctta catattttgc gcacttgact ggatatctcc
    45001 ctattgttgt acctcttttg ctttgtcctt ttctgtttgg aactttctta gcatacttaa
    45061 caagtggaag gtagtccttc tttgcttcta tgtctgctaa aatctttaat gcttttttta
    45121 caggcatatt tctaaaataa tttccaaact ctactgcgta cttaacagag attgggcaat
    45181 tatgctttga agctcttgca gactttttag agtccaactt tttttggtag ttgtaattat
    45241 gatctgatga catattttat cactttactt tcttgcagca acagcagtac tggacttggt
    45301 tgcaccgatt cctgctttac cgtgtcttat tctctttctt gtaaatgcaa actctccaag
    45361 ataatgtgct aacatgtctg ggaaaatttc tgtttgtaaa aactcttttc cattatagac
    45421 ataaattgtt ttaccaacca tctttggtgt aattatgatg tctcttctgt gtgttcttat
    45481 tttcttgttt gcttttttgt ctttttctat tctttcgtag aatgcatcgc tatggtttct
    45541 tcttaaatgc ctctttgctc tagttcctgc aatatttgca aaatctagta aagacatctt
    45601 ttttagttct tctagagtgt gccctctata tttgaattct tctgccattg tttatacacc
    45661 ttaattattt atactatttc ttctttctac ctgttctctt tgaagcaatt gcaccaacct
    45721 ttcttccaga tggagcgtgt cttgaagtag acttagagtg accaacgtga tgctgagaac
    45781 caccaaatgg gtgatctaca ggattcattg ctacacctct tactcttgga tacttcttcc
    45841 cttttgcttt cattgcttta aactttgcac cagcagtaac aaatggtttt gtttttcttc
    45901 cgccgcctgc aattacgcca attgttgctc ttgcatcaag tggcaattgt actaacttac
    45961 ctgatggtaa tttaattatt gctttttctt ttttgttaac aagtattgct gtctgaccgc
    46021 cagctctaac aaactttccc ccatctccaa agtttttttc aatattaaag attgtggttc
    46081 cgggctctat ttcgcctagt ggtaagacat tacctttttt cttttgtgca cctgagccaa
    46141 actcaagtct ttgacctgtt actaatcctt ctgcacaaat tacatattct tttgtgccgt
    46201 ctgccattag aatttcagca actggcgaag atcttattgg atctgttact aaatctataa
    46261 cttgtcctgt ctgtaattct ttaacagttc tataattaat atcaaattta gcattattgg
    46321 tagcagtata tactggcccg ccttttcctc ttctttgaat ctttaaacgt cttcccattt
    46381 ttaatcacgt atttatatga tacctaattt gttagctaaa tcttgagcat taaagccttt
    46441 ctttaaagta acaaatgctt tctttctgtt ttttctatca aataaaacat ttactgtttt
    46501 tactttaaca ttatataatt tttcaacagc ctcttttatt tctttttttg ttgatgcttt
    46561 gtttacaaca aaagttactc tgttggttgt tgaaatcata tttactgttt tttcagtaat
    46621 gactggatat tctaaaactt cataatattc gatttttttc ataaaatatc acttattcaa
    46681 atcttttctt taattcctca attgcagctt ggctatagat tgttagtctt cctgcgtgtg
    46741 ttcctggagc taactcttta attgataatt gtcttgcaga gatgacatca gcaccttcaa
    46801 gattactgaa tgctttgtag tttttactgt ctgctaggac aaacaaaata ctttttcttt
    46861 tcttgtattt tcttcctctc atctttcctt ttcctgctct tattgttttc ttttctttag
    46921 cttttattac atctttatct aaacctaatt tttcaagagc tgtatagatt tgagaagtct
    46981 tgcttaagct ttctaatttg ttatcaaaaa taattggtaa tgttatcttt tctggtaaga
    47041 tgtgtcctct ctcttcaact aagtttctat tagctgttgc agaaactgca gagattattg
    47101 cttttgttct ttctttagag tttattttcc ttgcaaggat ttcggcaacc tttggagggt
    47161 gagctcgtgg tccaccaact gcttgagaga ctctcataac tctacctgcc attaacactc
    47221 ttttgttctt tgttcttggt agtcttgcgt gcccagtatt aattgtgatg tttgctttgt
    47281 tttcttttct tctaccgatg tagattgaag tatagtttct gtttgagtat ggagaaacac
    47341 ccattgcctg atagcctttt gactgactag agattactgc ttttttaatt aagtctggtc
    47401 tatagtcctg tgcaaaatat tcaggtaact ctatctcttt gattgtcttt ccttctaaat
    47461 cataaatatt tgctttcata gtaaatcaat acctttaatc tttacttgtt aatataactt
    47521 atatctgtta attctaactt ttgcttttgt ggtctaaatg tgtgtcttaa agccactact
    47581 ctttttgtgg ttccaggagt ggagccatca acaagaatat atttgttttt taaaagtccg
    47641 tagtttttta gtccgccctt tctattgact ttattaatgt ctgaatctat cataactagt
    47701 tttttattag tctctgttct gttatgatat ccatattgac cagctcttgc aacagtatac
    47761 atgattgttg caggatgcca tgggttgata ccaccaacat gtctttgaga cttgtttgct
    47821 tttcttcttt gtactttaac attaaatctc tcaacaacgc ctgttgttcc ataacctgtg
    47881 gttatgcttt tgacatctag ataatcatca accttaaaga cgtcatcaac tgaaattgtt
    47941 ttgtttattt tttcttttaa gtaggatatt tgctcatcat aggttcctga caatggaatt
    48001 tcaacaatta ctggcttttt ctggcctgtt cctgttgcct tataattgat tgttgctatt
    48061 aaaactaaag aatcagcttc atcttttctt ttaagagcat cttcaagagt tgcggatttt
    48121 ttttgttttc tacctgtaac tctcttttta aaatcagtgt cttggtttgt aaactcaatt
    48181 attgcttttt taccggcatt gacattatgc tctttataga atcttgcgcc tataacttta
    48241 agatcagggg tctctatgat tgttactgga acagagattt cttgaccata ggaggatgcg
    48301 cctttttttg cattcttgcc tatcacaaaa gtgctgcctg ccttgattgc gtaaaaacaa
    48361 agaggcttag tacccttaat tgtcttctct actcctccaa aggagttaaa cttaggaata
    48421 atgctactag ctctaactct tggataaaaa ctcattgaac cagatcttgg tttattttgt
    48481 acacccataa taatcgtact ttgtgtgtta tattttcatt tttcatttaa ttaatattaa
    48541 aatccgtaga aatagcataa aaagatagag aaaaactcat cttttctgca tttatatgtt
    48601 ggttagtgtc tttctcgcca ctgattttgg taccaacaaa cagtctacag attgtggttt
    48661 aaatgcctaa aaaacaaaca aataagcttt gtttgtaaat cgaaggtcaa ttaaaggtct
    48721 ttttctttaa cctgtcaaca tcaaagctgt cgtcagatta aaaatataat attatagaat
    48781 aattatagaa tcaaaatgtt tataaataga aaggaaaaaa aagaaaaa
//
LOCUS       DAOOON010000004        43907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_967_length_43907_cov_18.153060, whole genome shotgun sequence.
ACCESSION   DAOOON010000004 DAOOON010000000
VERSION     DAOOON010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 43907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43907
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_43907_cov_18.153060"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLK55_01150"
     CDS             complement(<1..196)
                     /locus_tag="PLK55_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35574.1"
                     /translation="MRLSKQKPLNWFTELFTIGYLKTHTFSTIFIYAILIIAIIGFIS
                     VMKSFTPLALGVFVLKLIFAL"
     gene            complement(235..567)
                     /locus_tag="PLK55_01155"
     CDS             complement(235..567)
                     /locus_tag="PLK55_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ35575.1"
                     /translation="MTVILCNRHNFEEEVVESDVPVVIYFWETLSGPSRALNLTFEEL
                     SNEYENKIKFLKINSEENKPISTMFKIGEVPCIVIMKRGAEIGRIIGFEPKPQLKQKI
                     DHVLSKVR"
     gene            complement(688..831)
                     /locus_tag="PLK55_01160"
     CDS             complement(688..831)
                     /locus_tag="PLK55_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35576.1"
                     /translation="MKYETKMCPFCKNVTRHRIDEESGDEVCLKCNNEPPKVKDYKFK
                     GFR"
     gene            complement(1470..1934)
                     /gene="ndk"
                     /locus_tag="PLK55_01165"
     CDS             complement(1470..1934)
                     /gene="ndk"
                     /locus_tag="PLK55_01165"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOZ35577.1"
                     /translation="MMERTLVIFKPDALNRALVGEILHIFERKGLKIVGMKMTHLSDD
                     LLDEHYSHLKNKPFFPRIKKFMKSAPSILCVLEGNNAVTVVRSVCGVTRGYEAAPGTI
                     RGDYSLSGQCNVVHASDSLVAADIEIARFFKDEELFDYEKIDFNFIYAEDEK"
     gene            complement(1947..3140)
                     /locus_tag="PLK55_01170"
     CDS             complement(1947..3140)
                     /locus_tag="PLK55_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35578.1"
                     /translation="MYLFLLGRDKDLSKLEIAVYLHKHNISYSVIANSEKYIILDFNK
                     EHNFEGLTKELGGLVRVSKVYLETSKIKSNKEIINKLNFDSPKKFNYTISTIDILEDE
                     LEIVEDLLKQEFKADRAKAVYKKPVTHSKKRSKENINYIANPDNYFSWKINDGLELFV
                     VKFNNKFYFAKTTSCFNPKDNIFKDKNRPVIKEMYNTSFRLVSIMINLLGQPKGKTIV
                     DPFCGTGTFLIEGLIKNFNVVGIDNSKEMIEASNKNVSWAIKEFKLSSRYKIIQGDSA
                     KTRFSADACVFEPYMGPFLKKALNLEKARNIVKELNSIYYAVFANLNNNLLKKARVVC
                     ILPDFKTYDNKVISINRGVYERNGFFLVDVSVLNPKLELQNPIAYTTPSGSMINRKIY
                     ILEKK"
     gene            complement(3204..3947)
                     /locus_tag="PLK55_01175"
     CDS             complement(3204..3947)
                     /locus_tag="PLK55_01175"
                     /inference="COORDINATES: protein motif:HMM:NF003239.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOZ35579.1"
                     /translation="MGLRPGHCYCPRVNKRAYTRIAVKVHKKNFIGGVPGLKIRQFHM
                     GTGTKNYTHVVHYVTKEKVQIRDNAIEAVRQSVVRQLNKKLTAVNYYMKVRVYPQFFL
                     RENKQAQGAHADRIQTGMSQCPFGKVVGRASRVTKGQKIYSVLVDEANVKMVKELFER
                     MASKFPCKMKVIVEKNKSNLKSVGTVRKKKSKIKEEEVTPKATDAAATAAATTAAGKG
                     ATPAKGATPAKGAAPAAPAGKAATPAKKK"
     gene            complement(4422..4772)
                     /locus_tag="PLK55_01180"
     CDS             complement(4422..4772)
                     /locus_tag="PLK55_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOZ35580.1"
                     /translation="MIRNISQKKNIIATHRIADNVFSRAKGLMFSREKNFDYGLIFDL
                     ERDTHIGASIHMFFVFFPINVVFLDSKKKIIDIKLGLKPWQMTKPRDKCRYIIELPAK
                     YGKKLFSIGDKLNW"
     gene            complement(4792..5919)
                     /locus_tag="PLK55_01185"
     CDS             complement(4792..5919)
                     /locus_tag="PLK55_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ35581.1"
                     /translation="MIINPQENDFFKKANIFFYNNEYSILELAGIVCGLLIIAIANKY
                     FAASNIYFIVFISGIFVGGLGFLIEYYIKTEKRKSVETEFNYFLYDLSKEYKKTRNLS
                     LALSNLSEYNFYGNINPEIKRLSNRVSWGESFEESLYSINKNINSSVIEHTLIIIDVL
                     KKSTIDYDKILENVSKDLLLFRSESKNKIYFSNLFYLSVLFYFIFIFVLLYIDHIIGS
                     NFLWYSTEEIITRTFFDNFILYIALLLGGFTSYVMYIIKKDRGLNFIKYVFILFVVTI
                     VLFQTFIPKPDAEEIIIDTMEYMIKNNKTEVEMRHIIALKTISSKYILESTRINISFI
                     DSNCLTDCKTYTILVNEPAFYDFKITKQQDNEFVITFGLSK"
     gene            complement(5924..6766)
                     /locus_tag="PLK55_01190"
     CDS             complement(5924..6766)
                     /locus_tag="PLK55_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ35582.1"
                     /translation="MFGKTLSKISNFFFSDISKSIAPKFKELTKMLVITNIEEDVQTY
                     ISNTLFLSFLVGICLEFFMIFVMLKLNILFSVFTFVLTIIIAFTFAGFIFMTLYRYPH
                     YLITSNKKELEHELEISIRHLSVLQDQKMSVKDVLYLFQKIGSNKLLTEKSKKILAMS
                     DLNNNLKGTLKQICNSTYSEQEYNFFSKLIDVLENKTTLSNVISDYLESTDQLRKEKE
                     ERKRSKITLLFEINIFLFFLIFILIFSVFLMPFYRDSIKNILFIIAIAFPIVEIILVL
                     ILHK"
     gene            complement(6788..9307)
                     /locus_tag="PLK55_01195"
     CDS             complement(6788..9307)
                     /locus_tag="PLK55_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35583.1"
                     /translation="MVELKKLSRINDNSDPKKIEKTLTQKFNISIDNTPPDSYQNKVG
                     SLPKLKLHNVSEKKEEFRNFKIETKTHENNSESNYPTIETITPREYVENALRLEVEKK
                     RLEVISNKLQDIAERITITNLENVVEKDTPEVDFLLTALEEKTDKVEDEQPFFESISF
                     LDDDPKIKKIAIKDTGVSHLYAKLDAIETKVINQKKIKKEESQYNGEQQNKEDQKNTN
                     NIYALLEEIEVPEIFSIKDDTNISEAVNKGFSTYEQIPPTKKIRESTFILDNGKINTN
                     KNIDNNISNYKKEQQKAKSEKPKLNIDNYIEDIVVPDLSFDNNEIKEKSLIEEKPNQT
                     KVSQKTSFDYVLPKDAEERTLSKTAISRITKETIDKPCVQQSSSITKVYSVKPQISET
                     DKINISEKYNIDEFTFVNINYMMSQGLVYSVIQPELNQSQEETYVEIKKIFLDSIDSN
                     YTSFKGDKQNLNNYIKKVFDLTIDKLSYNLTDLEKKLYFNFIKRDFLGFGFLSTILED
                     KNIIEISCSGENMPIIVYHLKYGAIETNLKFENLFKLNQFVLSLTKIMGLQVNSAQPI
                     INGYLPNGYKVEGLYSVGDISNRGSSFIIKKYLEEPLTPVSLINLGIGITDIYSYIWT
                     AMDDDFQILITGGDANLFISALAQFYPDKTITSIQSFDYIKLPQKNWIKKLLLDNIAS
                     NKKTIISQTLSQRSDYLILDNFSEDLFDIKWYDFSMVYLPEELLPKYSEKAKAINRKA
                     IIISLERTKINNIEQMQIIKILEISNGKDNDVIYYSKKDSDFKINLESSAISTSEFFN
                     HKKLLRWLVDSGITDGTDFNNIVNDYYNNKKQLLKKLNILE"
     gene            complement(9384..10097)
                     /locus_tag="PLK55_01200"
     CDS             complement(9384..10097)
                     /locus_tag="PLK55_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ35584.1"
                     /translation="MFLIGLICFIYLGYQDLKYREINIVPLLVISAIGFAYYIFFGLI
                     KGFLYMYILQIAISFAFLTIIYVLGKFTVYAYIGEGDLIALMTMSFISGISVLFTGLV
                     FLMSTVLTLFFPILFLIYNTIKGNFPKKPLGESLILMSFGFPKAINKLTNFYTPLEEY
                     KLVNNKVISKTTIKPNCSPEQQIYELKKFSKIHRIDKIWVSPLVPFVLILTIAYAIVS
                     VLTYFNVLSLLGNFATSFI"
     gene            complement(10121..11095)
                     /locus_tag="PLK55_01205"
     CDS             complement(10121..11095)
                     /locus_tag="PLK55_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35585.1"
                     /translation="MSYNLDLNTQFLKRKQSSKRYWQFANKNNLLGTAITENAKKNPK
                     KAYFDLLKDKPKANILIPGITISTDQGEVLVYAPNEDIYSYNVFFEENVSMRRIYDMC
                     ERKDYLFVIAHPFGLINNSASYLMGLSKLEKFIKEKNVGVEGYNGMMGYITYYMYDSF
                     FIKTLRDFLWYVQKHKAFNIAGISKTADRLNQKIDQKSYDLVYKFSATIKLAQMANFV
                     TAGSGTNYVDRIGSGVLTIDLPKSIIDETNNTLQNQAILNKILKKQIVSFGPPGKYTD
                     QLFERSTARITKRKAYDDLIHLTKTAIQTKKPKKIKEKKPIKEKEEKR"
     gene            complement(11105..11722)
                     /locus_tag="PLK55_01210"
     CDS             complement(11105..11722)
                     /locus_tag="PLK55_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOZ35586.1"
                     /translation="MAWLNLEDSFKYLKQKNIAILESKELLKKDIERFSDFPYVLKLS
                     SSLVHKTESKAVYTEIYTKEAFIDAFEKLQEVLKKEKVKGKVIIQKQVKGLELLIGIN
                     NDAQFGKVIVFGQGGIYTELQDDTTIRLLPITKKEIEVMVYTTKVSRYFSARGKNYPV
                     SKLISLLEKVCELSKDKHLQEIDLNPVILTEKEIYIVDARVNLKK"
     gene            11944..13248
                     /locus_tag="PLK55_01215"
     CDS             11944..13248
                     /locus_tag="PLK55_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOZ35587.1"
                     /translation="MKLHCLGAGQEVGRSALLLKGKNENILLDYGLKLNPTTHVKADM
                     ELPAKTEHPKAIKEPLAGIILSHAHLDHSGAIPSLYKEANVPLFLTEATLELSNLLWK
                     DTLKIAKLTGEDCGFKPQQVDDTNNSAFYIDYRVPVQISPNIKITFFDAGHIVGSVMA
                     LIEMDKKKILYTGDFRATESQMFRGYDRKLPQVDILITETTYADAPHKNRKEQEKLLV
                     DEIRETLDNRGIVILPAFAIERSQELVSILDRYNIKVPVYLDGMAKKATTIFAQYPNQ
                     FVEYARFKKAVDRIEFIKNQKKREKIVATNTPCIIITTAGMLEGGPVLGYIRDLGDDP
                     RNKIILTGYQVEGTNGRRLFDKGKLYIDGKIYSPRAKVTKHSFSAHADKLELLELVKK
                     VNPKKVICIHGDKDVTFDFKNELKKLGINAENPAVGDIVDLD"
     gene            13263..14036
                     /locus_tag="PLK55_01220"
     CDS             13263..14036
                     /locus_tag="PLK55_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMD3-related protein"
                     /protein_id="HOZ35588.1"
                     /translation="MKRFCPKCGKTVPEERMINNFCLNCYIEDHQVITAPTFEITYCP
                     KCKKLRYARKIFDNVENLEKELVKHIRIKDLEQAKANVTLVLDFDKHEYYVKVLVKAL
                     IANTIQEIAMEKDVSLRVDNCENCLKLSSNYYTAILQLRFDTKELLKELQPVLVNQIE
                     NQVKTINNADSNKNKPFFIAKTIEQKTGIDIYFNDSDHAEKVRKTLLYNKHVKTWQKT
                     RSLITADKDGKRKYRHTICLHYGVLPTESGAKELEEIEN"
     gene            complement(14033..14689)
                     /locus_tag="PLK55_01225"
     CDS             complement(14033..14689)
                     /locus_tag="PLK55_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOZ35589.1"
                     /translation="MYVIIVGRSAVSKHLIDLLDAEENEVVVIEKDPAISKAIAYEFD
                     VVTINGEATNINTLKKAGIDHADVFLALTDDDETNLIIALIAKQAGVKTVAVILRKIG
                     YQKDVFDKLGIDYVIQPDYAAAGYIAQLITDKDILELSFYSMGDAAIVETLVKKDAKH
                     IGLHISKLKELLPEGCNIIGYFKNSEFYVYKEKDKLEEGQKILIVAKREKIKIIKELF
                     "
     gene            complement(14691..>15014)
                     /locus_tag="PLK55_01230"
     CDS             complement(14691..>15014)
                     /locus_tag="PLK55_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOZ35590.1"
                     /translation="ASLPKKHNGEAVDNEDIKFLYFFIISYIILIGITVLILVAYNYP
                     ISHSIFEVVSAQGNVGITTGITSHTMPIVPKIALILNMWIGRLEIIPIFGLMGMLLQK
                     KLKVK"
     assembly_gap    15015..15114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15115..15764)
                     /locus_tag="PLK55_01235"
     CDS             complement(<15115..15764)
                     /locus_tag="PLK55_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOZ35591.1"
                     /translation="MYYTRWYFNEIFRTIGLIIYYSSFLFLIPLIFSIIFKEGNVVIF
                     GYLATFIISFLIGLIWVKLIKKKEIIHLNLIHYLVIVCGVWLIFVALASLPLVFLGYS
                     FLDSFFEMMSLITTTGLTTLPNIPILKSINFWRAFVAWIGGIGIIFIAYFGLINSEIL
                     NTKKIVKAEGHDFIHQNPKKTAREFGFLYIGLTLIGIIALFIAGVDLFSASTFIMSA"
     gene            complement(15838..16326)
                     /locus_tag="PLK55_01240"
     CDS             complement(15838..16326)
                     /locus_tag="PLK55_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35592.1"
                     /translation="MSSKLFKNPILEKIISGIGIIVIGYVLLILTFLLAAVFQGLLSR
                     LFGLFIQFNEHSPYIIIPGIMRILFLIFIFLISWLILHKSKLPDFWKATFMVVPLATA
                     LVTIGIFFYQTPAIVYLLGLIFGIAIFYYLYKTKKSWLYYFSLFFISILMLLVQFLKI
                     EI"
     gene            complement(16356..16430)
                     /locus_tag="PLK55_01245"
     tRNA            complement(16356..16430)
                     /locus_tag="PLK55_01245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16393..16395),aa:Arg,seq:cct)
     gene            16526..17095
                     /locus_tag="PLK55_01250"
     CDS             16526..17095
                     /locus_tag="PLK55_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOZ35593.1"
                     /translation="MSKKILIMLGPAGSGKSTQDKLIENEFGYKPFIMGDILREEVKK
                     GTTLGKRIHALIKDGHLVTLEMASDLIFDKVKKSKAKKIIMDGFPREYYQSIVFDYFI
                     KTNNYKLMGVIHLNLSRNESIKRLLLRKRNDDELKAINQRLDDYQGITIKAIDRYKKE
                     NKIIEVNGEQKIDCVFKEIKTKLNKIKGI"
     gene            17099..18733
                     /locus_tag="PLK55_01255"
     CDS             17099..18733
                     /locus_tag="PLK55_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35594.1"
                     /translation="MIQDPRIIDNIKKLKELGMSNDDIKDNLIKMGLSSDDCDELIAA
                     SNEDENKNRKLQEKEQKKEKIKKEEIFSEPKKNEPAEIPETLFQEDNKIDALKEEVSF
                     TIKDDFSEIPDLTSGLDVDQLNNSDTDINFNAPDLSREEEKIKFEKPKNKDFDIIKSI
                     DLDKINAAKLIDTTPSETLKELDANSNIWGSGLATTLNARLNEIDEKQENFEKLLKKK
                     MEDEVKKFKQIQETSKQLLAQKIDEELASRTANINSQLTKQLAQIKVELIKINKKSDQ
                     MTTGKQEVEDLIKRLKEIQTQVLENNNKSQENINRMVLSTSVKLNTKIKEINDILALQ
                     SKITQGLIKNTQTAITNEIKKFNQFKEELENKINPQQMYDKLNQLETFKQQLASRYET
                     RFENVKREFLIKAKDAFKEEIETQLKELKNIKETIVAKTDPKLISDKLEELESFQNHL
                     ISAIDEKISQSLKIYQSAMTQDISSKAKTIDEEVVKVENAIVKLGDAEDKIKELDQFK
                     DQFIAIIDKNIERMNATYSKFEQKLKEIEDKQKLMI"
     gene            complement(18734..20614)
                     /locus_tag="PLK55_01260"
     CDS             complement(18734..20614)
                     /locus_tag="PLK55_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35595.1"
                     /translation="MQTKLNDFQKYNKKSSHNKFKYLVIGIFILLSLFTAKAVFAETN
                     EPEIGNLDLIFDKGIYSLMQDTSTKIGFTIVNNNPVVAKIRVWADCEDEDELECNYSK
                     RYTLPGNSELTDSFYVSALDESDSKVTVYIKLLNSEDQDTEEFRTDIEVTEDEEDGEF
                     SVDLSTTNVCIGRTNTITLDIENDYSDGLYGIYLSSPKLVISSEYSNPVYLRDEKEID
                     YSVIVSNNATDGEEFDLTLRIENEEIKIIKEFSLYAEDCPEPNNDFTVSGSASTTQNI
                     SKGQSKTLSYTLKNISNTNRTFYISEDNVNSEIKVDISSRQFNLDPNQSKNIVFTFTV
                     SDKIISGTYNLKLNFFDGLKSISKNVKLVVNPKFDFKIENLSESNLNLAIGKTIQIMI
                     LLKNTGDVKENITIELNPNNDLEATTQDSRITVDKHNNEYVTIYVSAGDFTQLGLSSL
                     NVKINGTSNNFSEELDYIINVVKISEPLNIELLAYPKTLSVEINSSKEFEITLRNKGN
                     KITINKIELTNIPQNIDLVVDQTITLNPGETKTLSAKINIGDIPKEDIEAELRFVSNN
                     GEVLEQPILLKLTEDVEKDKSQSKLLGFLTLRNSVWGGIIFICLLIMLFFILGIFKIK
                     RN"
     gene            complement(20628..21467)
                     /locus_tag="PLK55_01265"
     CDS             complement(20628..21467)
                     /locus_tag="PLK55_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35596.1"
                     /translation="MIKKNVFLVLMITLLFSLQIIYAENTPTVSFPKKVTVVDTPIYV
                     TLYVYNPNSTEQTYSLVSYTSPYESYFSEDKITLKSSESKEVTLIISPQENVLDSTYK
                     ASIELMYLQNSKKIEFEIIQKANRSCPIDLQHTIVYVKESGNYRLDLFFNNSSKKDQE
                     LDVIGIKDINLEYPIGVVIVPSDVETKMVRVFKTDLKETKIEYRCNGVYGYVTLELPK
                     KETDKSKVQVISVFTGLVSFTKSINLTTIFNSIIFQIILIIILIIVILSFSTKYIKYV
                     YRK"
     gene            complement(21589..24840)
                     /locus_tag="PLK55_01270"
     CDS             complement(21589..24840)
                     /locus_tag="PLK55_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HOZ35597.1"
                     /translation="MHIKELVLKNFKSFDYTTIPFKPGFQVIVGPNGSGKSNIIDALI
                     FGFGSTSLKRLRVDKTANLVNQSAKDRTARVRVVFNHEGKDIDIIREIDESGKSVCIL
                     DGKRKALNEITSFLEETGITADGYYTVQQGDVTKIINMNPEERRKIIEDISGISLFDE
                     RKKEAQDNLDKVNKRLDKVSIALNERKPYVEQLSAEREAALKFKELDSLEHIYSYNLY
                     NKQTDEYNSELFLEEAKITKLISDIDVKNQKKKELMLLISEYENKLENINNDLIAHSE
                     KVQSTVGRELSENKANKEIIQNNLNLRKENIEYLENENQRLSGELSELKKEEQLVKAI
                     IKEQEGKLKLKSKTKDNLKEQINKHSKEFEGVRKIQEELYTRLSSINKDITQKQEDFF
                     SNKTKMSAYEFQKETIEKQSKEKEAETKALLERKKEIEGIISKQRKEIATLESEISKL
                     FQKLKIEEKEKEDLQKVISQKTIELNGFRKDLSFSSLALDKREKLKANLKKHKSFIGF
                     LDEFVTLEKSQKSIYSNYVILKDSKDIPVILKEIGEHTNINFVVLTTLDISEKELSKI
                     FSEHAGTKTKAQSIDGFYFDGFCYKKILIKDPKELESKISATEKELEKAKSKLLEIIK
                     CHETSNKGHFEKNKELTDLQVKLNTNIAALDEIIEKTDNIKSASTSTTTIKTITQNLG
                     QIKDETSALDNILVTLRKEKQEIEDKLKSLNLGAQTTLRDEYDSLVSETNILEQSLIS
                     KNAEEKTVKEKIDSKSSLIEINNSKLKELKTQMNNYSDQILEIDNKIKEINKKFQTED
                     QKKQDMFQKKSKVSQESSLINQQIHEIDVSISEINLEINDARLNINTLKSKVLQADQS
                     LKMLEVKEKYETVEFTIEQLYSKLRAIKRDKNALGNINFNAIDSYEKLAKEYAEIIEK
                     FDIIVAEKAQVEAMLNEINMKKTTIFMDCFNKVNAEFKNVISKMSKSLKGSLELSGND
                     PLTAQLMINITKNGQTKNIDIISGGEKTVTALAVIFAVHAYKKSPFYILDEVDAALDD
                     QNSESLLNYIKELSKSVAVICITHNSTIVSGANQVIGVTLKGNSSVIGLDMAR"
     gene            complement(24855..25382)
                     /locus_tag="PLK55_01275"
     CDS             complement(24855..25382)
                     /locus_tag="PLK55_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOZ35598.1"
                     /translation="MSDSIKRVMIFIDGSNFYYSLRNSFYKTKIDFEKFCNFLSENDD
                     LIVIKYYNSVLNIDDDEEAYKKQQLFFDSLREISVMDIFFGRLEKRPDGKKSEKGVDV
                     KLAVDMVVNAYKDNYDIAILVSNDADFIPAIEEIKALGKQVINVSFPKTKSYHLNKVC
                     SKTIKINDISNYLRE"
     gene            complement(25675..26703)
                     /locus_tag="PLK55_01280"
     CDS             complement(25675..26703)
                     /locus_tag="PLK55_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOZ35599.1"
                     /translation="MKTYNNLWSKITSFKNLVLAYNKARKAKPQTKETLEFSLNLEKN
                     LLLIQEELLANTYTQSKYREFIVFDPKQRKIKYLPFKDRVVHHALVNVIEPIFDKRLI
                     STTYACRKNKGTHKALKDIKKTISREFRFTGYCLKCDVKKYFPSIDKIILKQVIEKKI
                     RCKNTKKLIFDIIDSDYSNFGEDKGIPIGNLTSQLFSNIYLNELDQYIKHKLKIKYYY
                     RYVDDFIILEDSKKQLNYYKKNIKYYLKKRLKLEIPKQKINLFEIKTGVDFVGYKIYP
                     EYVFLRKRNIKKFIKKLKILTYEKNFFKVKSSISSYLGYFKFANSYKILDQIWANNPY
                     FLKIKRLL"
     gene            complement(27114..27446)
                     /gene="avd"
                     /locus_tag="PLK55_01285"
     CDS             complement(27114..27446)
                     /gene="avd"
                     /locus_tag="PLK55_01285"
                     /inference="COORDINATES: protein motif:HMM:NF033474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HOZ35600.1"
                     /translation="MLDDLKVYKESYDFFLWLWPLINKFPKSEKYVLGEKIKLCFIAF
                     FENIVSYNYNFERQESLKIASVEIEKLRLFIRLSKDLNLINFNKYEIASRKINEIGKL
                     LGGLLKTT"
     gene            complement(27451..27831)
                     /locus_tag="PLK55_01290"
     CDS             complement(27451..27831)
                     /locus_tag="PLK55_01290"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOZ35601.1"
                     /translation="MWISDKGVASFKIYQDMFPVVEKILTILNDCENKNNDVYFYFLI
                     EDTNKTAIEILSYVASGYNNYDTNLKAIEYNKARNAISITQSNLILLNNSKLISDEDL
                     KENYDALEEKLKMISGVLRKIQKR"
     gene            complement(27990..29339)
                     /locus_tag="PLK55_01295"
     CDS             complement(27990..29339)
                     /locus_tag="PLK55_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35602.1"
                     /translation="MKNSKAKAITPIVATILLVVVAVILVTIVLNWGKNFTNSSVSKT
                     DSLVNYNVLNDKQSFMRFKSAQNGMYIFDYYPPSNTDINFKVVGYSLLGYNDYIPLEP
                     EKEITHAGEFMLPLGIINEEKISIALLLEDGSYLTFNNVKNTNRSPAPSQCPTGFVPV
                     PGNYLYGTEGFCVAKYEMKVDENGDGLGDTNASCKNELYLTWQNNINASCKYSVPSRT
                     IVSSAEGYPLTDINQTDSILACESIGGHLITNEEWMTIARNIEVVPSNWSSGAVGQGS
                     LKRGNAGDVVANVSYDGADPESGTGRNQLAMLKLTNGSEVWDLSGNVLDWTDRTTSDF
                     YLDCNKLIGSDFGDCDERKGGFEYNGSFENYSDAFDYVDFTKIGYKELFLLNSDYNKD
                     NGLGYVSFFMDSGPPHAFLRGGDWSAATTAGVLYLYAYSEPSTTNIAFGFRCVVEPT"
     gene            complement(29440..30729)
                     /gene="serS"
                     /locus_tag="PLK55_01300"
     CDS             complement(29440..30729)
                     /gene="serS"
                     /locus_tag="PLK55_01300"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOZ35603.1"
                     /translation="MLELKFIRENSKKVKENIKKKGKDILLPLVDEIIRLDEEYRANI
                     QVANDLKHKRNSVTDKIAENKQKGLPTEDLLAEARKFPDQIKVIDDKQKELFERIIDI
                     QKKIPNIMSRKTPIGKDETENKEIKRFGEPEKKDFEVLNHGELSEKLNVADFEASRNV
                     SGKGFYYLKGDLALLNRALINYAQDFLNKKGFTYVLTPLMINKKACEGSVDFSFFKEM
                     AYKIEGEDLYMIATSEHPIISMFLDKTIDEERLPIKIFGFSSCFRKEIGSHGLDERGL
                     FRLHQFDKVEQIIICKPEQSEKLYEELIKNTVKIFTSMELPTRTLEICSGDLGDLKHR
                     SADIEVWSPRNNKYIEVASCSNLTSAQAVRLNIKAKTKQGEKYFVHTLNDTAIATSRA
                     LVAILENHQTKEGTIKIPKVLQKYMYGKTEIKRNDIF"
     gene            complement(30848..31420)
                     /locus_tag="PLK55_01305"
     CDS             complement(30848..31420)
                     /locus_tag="PLK55_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOZ35604.1"
                     /translation="MSVKENDWVSVEYEGTLSDGQVFDSSKGREPLKFKVGIGMVIPG
                     FNNGIIGMEVGEEKEIKVSPKEGYGEKNTEVAKLPKELLSGISDLQEGKEIIVMSGMG
                     PLAIDIIKIEETTIDAILNHPLAGKNLIFKIKLLEILSKEEAEEMDKMLSQACDSDCS
                     HCQGECGEEHEEHCDCGEEDCDCEEEHCQL"
     gene            complement(31537..31722)
                     /locus_tag="PLK55_01310"
     CDS             complement(31537..31722)
                     /locus_tag="PLK55_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOZ35605.1"
                     /translation="MEVLNTIIGIVALICAIWVIYDVFTNNKKLKTEIKIIWTIAAII
                     FSILTAIVYFIMYKMKK"
     gene            complement(31811..32332)
                     /locus_tag="PLK55_01315"
     CDS             complement(31811..32332)
                     /locus_tag="PLK55_01315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35606.1"
                     /translation="MKVKLKDKIFTYRFATEKDIDIINKFDINIYRQVNHDFYFRHTK
                     EILKNMISKDRKIMLVFFDKQLVAWAGIKIKAEPFYAKIYNFTEKQLSKSSVLIATAV
                     SPKFRGYGIQKFLIKKRIEYLKKKGKKYFLHSAHPENKYSINNMLESGSKFIKKDKSI
                     NDGRIVNYYLLKI"
     gene            complement(32310..32747)
                     /locus_tag="PLK55_01320"
     CDS             complement(32310..32747)
                     /locus_tag="PLK55_01320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ35607.1"
                     /translation="MQKKNLKKSTPTLIFGLLHKKDKKIIGVESLNNIHFRNKNCESG
                     SWISRKYWGSGLMAEARLEIFKLAFKDLKLHKINSMVLIDNIRSQKHIAKFGFKQQGY
                     FRDEVVIYGKFRNAYYYELLNKEFNYNKLKKKLLKSHESKTKR"
     gene            complement(32689..32937)
                     /locus_tag="PLK55_01325"
     CDS             complement(32689..32937)
                     /locus_tag="PLK55_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35608.1"
                     /translation="MKNQEFFLKYKNYKIETKHLILRPLKLSDANDIYQAMQDKIFSK
                     QIWIIPWPYKMSDATKFIKDAKKEFKEINTNTYLWIIA"
     gene            32991..33833
                     /locus_tag="PLK55_01330"
     CDS             32991..33833
                     /locus_tag="PLK55_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOZ35609.1"
                     /translation="MIAQNLYDKLEIDFILRRGLTDDWKEIGTEKYLSPNFKKRSMGI
                     VYDFSEDIKKVYTAVFPEDSVIKKILAKKETDVLLFVHHPMTWDITKKPIFTSISEKD
                     LKIFKERRISVYNLHVPLDNFGRYSTTNTLAKELGVKVETQFAPYFGARCGVIGTIDT
                     DKVSDIIKRFEKAVGHKIKFYKYGLEKIKDKRIALVAGGGNNLDILKTIPKGINLYVT
                     GITVKNDHSQKAHEYLKEKKINLLGGTHYSTEKFACIAMVEYFKKQGLPSEFIEGKPV
                     LEDI"
     gene            33900..36362
                     /locus_tag="PLK55_01335"
     CDS             33900..36362
                     /locus_tag="PLK55_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35610.1"
                     /translation="MMIMNRCFLLLFFLVLLIGNVYAITINEITITPDLDISYFGRYQ
                     IDANITEYSGNDVNLLVSAINGDGGTCWDYFVDGTCSSETPSTYSMDYNETTGFWHYD
                     RVYPDTIYPEIIFIDSPTNWNNAPSWVNLWRQNYHIFKMYNSFEMDSNMNLWIEFNTE
                     PVSTNNSGDLFVYVVGAGVPLSYFTSDWRDDSNTQLVGTISRNTAFNHDHTPSTHYVR
                     HHLIPITANPDGTIGTNHVNISDGNFYIVLYQDSTATNRGWNLAYKPTSLCDNTDNWF
                     TANRTGGNWNLPIHRSGCSDAHIHFARRETYNDGVNLEVTAVDGEDITTLDVNYYFAE
                     LPNLAPNETSFITPIPLATYDTNSIELSWNPATDPNNDDLTYDINYMDSDGNIFVIDN
                     NFTDTNISWDVSALEDGNYLILGSVCDTEPLCTDFNLSDYFYINRSEPIYSLSTITIA
                     SNYFYTDYAKVGSQINLSFISTGTLSEDLNVIFYANGVEVTGDINVTNNTNDYNATYV
                     IQEEDDDGIVGFTISATNLDYDYFDTTDDSYVTIDNTIADQDQDNIPDANDTFVGSLG
                     IVETEGIDDLNITIDDDTNIVGIFTGVQTIKIYDAQTPILEFDHNFSESTLDLSVIKI
                     IKTNAGVVVDLNGQLQEDQNKTLYLENNNFISLCVENEAITSISDISSNCLDANEYDF
                     TECLSESYSSNGIDCVLNGNLITVSNLRYSGILGTVRTTSGGNSISKEADDTEEIISE
                     INTDTENTDNNPIIVTPEDKDPEYNVVSSDSPDYKPNDEMPPIDYSRKSKNTTTIWII
                     TYFICALIIGITIYLFTKIRYR"
     gene            36475..37314
                     /gene="prs"
                     /locus_tag="PLK55_01340"
     CDS             36475..37314
                     /gene="prs"
                     /locus_tag="PLK55_01340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOZ35611.1"
                     /translation="MAKKLNLSFSKIQTTKFPDGEIDVTFGKDLKGKDVFIVQNFQNT
                     KSLTLNDKIVETLFAIYTARDLKAKRIYLIAPYFPYFRSDKRFRDGECVSIKVMNKLF
                     EGCDKIFCVDPHLHRIKNIKNALSNGIEINLDEPIIEYLKKKKIQKPIFVGPDIESLQ
                     WVVNVAKAFKEKPVVALKERKSAREVVISLPKIKSAKGRDIIIIDDIISTGHTIIENI
                     KQLKKYEPRKFYVIGIHGVFVEGALSKLKKHCFVASTNTLDNSAEKIDVSSQIAKIVR
                     KNI"
     gene            37311..37754
                     /locus_tag="PLK55_01345"
     CDS             37311..37754
                     /locus_tag="PLK55_01345"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOZ35612.1"
                     /translation="MTEKYFLASDHAGFELKEKIKEYLKTSKLDFQDLGPKTYNPEDD
                     YNDYAKKVVKKIKENKNHFGILVCGTGQGMSIQANRYSGIRCALCWNKEIARQAKEHL
                     NTNIISMPGKYINIKTAKEIIEIWRKTKTLSLEKHKRRINKLEIL"
     gene            37751..>37896
                     /locus_tag="PLK55_01350"
     CDS             37751..>37896
                     /locus_tag="PLK55_01350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOZ35613.1"
                     /translation="MKLQLALDVTDKKKALELCKKISKYIDIIELGTPFIKIYGLNGL
                     VKDF"
     assembly_gap    37897..37996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37997..38257
                     /locus_tag="PLK55_01355"
     CDS             <37997..38257
                     /locus_tag="PLK55_01355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOZ35614.1"
                     /translation="YKPDYIGIHSGIDMQHMGIKPLKTLIDLSKIMDSKKIAIAGGIN
                     LETIESIAKYKPGIIIVGGAITNAKDPVSVAKKMKEIMKKYE"
     gene            38250..38816
                     /locus_tag="PLK55_01360"
     CDS             38250..38816
                     /locus_tag="PLK55_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOZ35615.1"
                     /translation="MSKDIDDILERIVNEIKISLKDISEIKTSRFVKEILTSKKVFVT
                     GQGRSGLIAESFAMRLMQMGIKTYHIGDSTTPAISKNDLLVAISGSGKTEITNMIIKE
                     ADKAKAKVILITNKKTTKEKKRIRNTDLIIEINAKAKTDKKKKSIEPLGTLFEQASLV
                     YLDAVIVLLMERQNVKEKDLGKRHANLE"
     gene            38879..40318
                     /locus_tag="PLK55_01365"
     CDS             38879..40318
                     /locus_tag="PLK55_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35616.1"
                     /translation="MKTYVKKGKVSIDETTPEEILNPATFTNTIKKTALLKKINYDLE
                     KRETKPIDIKKTIVPEKKETIDIKENITREIKKIIIDNTEKQIIKKTYPNTELKHIPL
                     DKSYSEKKEIEDYNLKNEKINKKLNTGLSDRLKPKLKKIHRNPELTFYKSIDNPIICP
                     KPENGWESWQTFNPAAILIDDKVHFLYRAIGDDGMSRFGYAMSEDGFVIRERLSYPAY
                     VHEIVKGPYVFLSSPSGGSWGGCEDPRLVRVENEDRIYVTYTACDGGLRVGLTSIKVS
                     DFINKRWNWKKPKIISPPGECNKNWLIFPEKINGKYAILHSINSGKILIDYLDNLDFK
                     DGKFIKSKYLLTEVRADRWDKKVRAAGPPPIRTKYGWLLFYHAEDKDDPGKYKLGAML
                     LDINNPTKILYRCKQPVLEPKEYYECEGYKGGIVYALGSVIKDGKLIIYYGSADNFVC
                     VAYTEFEPFLEALIRGEKKPLKLKILKKK"
     gene            40331..42235
                     /locus_tag="PLK55_01370"
     CDS             40331..42235
                     /locus_tag="PLK55_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35617.1"
                     /translation="MIIKRYDKNPILKPNKNSCWESHSTFNGCAIKKKYETFLFYRAM
                     SCNDFHPVNEQKMEMSCIGVSRSFDGLNFKNRKVFIYPEESFDKFGCEDPRITKLQDK
                     YYIFYTALSEYPFRASGIKVAVAITKDLEKIEERHLVTPFNAKAMTMFPEKINNKIWC
                     MFTYHSDSPPAQVCFAIFDKEEDMWSRDFWQKWYNNHNKFVLPLQRREKDQVEIGAPP
                     IKTKHGWLLFYSYIYDYFSSNKVFSIEAVLLDLKDPTKIIARTKGPLLTTEEYYEKYG
                     MVNDIVFPSGATINGNYINLYYGAADTTCCVAFVDTKCLFEQMLPNTLNNISFIRSEK
                     NPIIKPNKKNKWEARSTFNPAAIYLDDKVHIVYRAVSDNNVSTMGYATSKDGITIDYR
                     SPEPIYKPRAEFESSGCEDPRLTQIGDNVYMLYTAYDVRTAPRVALTKISVKDFIANN
                     WDNWSKPILISPPGISDKDACFFPEKINGKYYVLHRLENEIDLCAIDDLEFKGKNKYL
                     EENNWMAPRKGWWDYRKVGAATPPIKTKEGWILLYHGIAEDHSYSVGAALLDLNDPTK
                     ILARTDEPIFKPEKDYEKSGDVPNVVFPCGAIVKDNKIYIYYGGGDSVTGVASMEIEL
                     LLKKLKLCRC"
     gene            42239..42571
                     /locus_tag="PLK55_01375"
     CDS             42239..42571
                     /locus_tag="PLK55_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOZ35618.1"
                     /translation="MKETKRPWGKFLTFAKNQKCTVKILEVNPGESLSLQKHKLREEH
                     WYFLDPGIVQIGNSKKKIAAGKEVIIKKNKEHRLVAGKNKTRVLEISFGNFSEKDEVR
                     LEDKYARR"
     gene            42558..43886
                     /locus_tag="PLK55_01380"
     CDS             42558..43886
                     /locus_tag="PLK55_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOZ35619.1"
                     /translation="MQEDKAFKTNDIRGIYPKEINESLAYLIGKAIVYKFKPKVVVIG
                     RDARASSPLLFSSVVKGVLESGCDVIDIGLTNTPLFYYSVGELKADFGIMITASHNPG
                     QYNGFKLVKKGVVPTTAKEIQDIKNIILEKKAISSKPKKLGKIIRKEISEKYLKKLLS
                     YSKIKKKLKVVVDTGNGMAAPIVSKAFSNLPISVIYINKKMDMIKPKHEANPLKIETL
                     KELQKEVKKNKADIGIAFDGDGDRVGFVDDKGEIIPMDFITALLAKYFLEKKPKSKII
                     YDIRSSNIVKETVLASVGIPVEYKVGHSLIKKKMREINAVFAGEISGHYYLPEFYYCE
                     APIFISIILMNYLEREKLSKMILPFRKYYKSPEINYKIKNPEKKIKELRNKYSSGKIK
                     TLDGLKIEYPDWWFNVRGSHTEPLLRLNLEANTKELMVKKLKEIEDIIKK"
BASE COUNT        15246 a         6668 c         6187 g        15606 t
ORIGIN      
        1 ctaatgcaaa tattaatttt aaaacaaaaa cacctaatgc caatggagta aaagacttca
       61 tcacagatat aaacccaatt attgcaataa ttagaattgc atatataaat attgtagaaa
      121 aggtgtgtgt tttaagatat ccaatagtaa aaagctctgt aaaccaattc aagggctttt
      181 gtttagataa tcgcatatat attatatgta taattatata taaaaatagg ttgtttatct
      241 aactttagaa agaacatgat ctattttttg tttaagttga ggctttggtt caaatcctat
      301 gattctacca atctctgctc ctctcttcat aatcacaata caaggtactt cgccaatttt
      361 aaacattgtc gaaatgggtt tgttttcttc gctatttatt tttaagaatt ttattttgtt
      421 ttcatactcg ttacttaatt cttcaaaggt aagattaagg gctcttgatg ggccagataa
      481 tgtttcccaa aaatatataa ccacaggtac atctgattca actacttctt cttcaaagtt
      541 atgtctatta caaagtataa ctgtcatttg tcttttcctt tgttttatat ttctaagata
      601 ataaatataa aatcatctat attaaaaaac ttactatttt ataaaaaaac aactattctg
      661 tgattaaaaa ataagaagaa aggattctta tctgaatcct ttaaatttat agtcttttac
      721 ctttggaggt tcgttattgc attttaagca aacttcgtct ccactttctt catctattct
      781 gtgtcttgta acatttttac aaaaaggaca catttttgtt tcatatttca tgttttatca
      841 caatttattc attcaattaa taaattcctt atcttggtat ttgaaatata ctaattgtta
      901 tatatctatg aaaagaaagg tttaaaatca cattttttta acaaataaga gttttaggat
      961 aaaagtttta gaataaaaga actagtctga taagaagaga ttaaaaaaaa taaaaagaat
     1021 aagaaaataa aaaaatcata tcaaaaaaat aaatcagtta gtttacgatt tatttcttct
     1081 tcttttttac tgtttttttt gctgcaggtt tttttgctgc ttttttcttt actaccattt
     1141 tctttcaccc ctttttcaac ttttccttcc ccttatggaa aaatattata tattaattat
     1201 gatacaacat atatataaag ctttttgttt gcttatttat gaaacctttt tcatgtttgc
     1261 ttgcagacaa atgaaaaata atacatgtct gtaacttcca ataaatacta attacggcga
     1321 gagttgtaaa cacgcatgaa tggcgtcaca tatattcttt aattggccta aagaaaataa
     1381 aaaatgaaaa aaaaaggttt cacggctaaa ttcttatatt aaaaaaaggt tttatgaaag
     1441 taaaacaaga aaaaaaatga aaaataattt tatttttcat cttctgcata gataaaattg
     1501 aaatctattt tttcgtaatc aaacaattct tcatctttga aaaatcttgc aatttctatg
     1561 tcagcagcaa ctaaagaatc tgaagcatga acaacattac actgcccact taatgaataa
     1621 tctcctctta ttgtgccagg agctgcctca taacctcttg taactccaca gaccgatcta
     1681 acaacagtaa ccgcattatt gccttcaaga acacaaagaa tggatggtgc acttttcata
     1741 aatttcttta ttcttgggaa aaatggttta tttttaagat gagaataatg ttcatctaaa
     1801 agatcatcgc ttaaatgagt cattttcatc ccaacaatct tgagtccttt tctttcaaag
     1861 atgtgcagga tttctccaac aagcgctctg ttaagtgcat ctggtttaaa aattactagt
     1921 gttctttcca ttatatctca tctcctttat ttcttttcta aaatgtaaat ttttctatta
     1981 atcattgaac cgctaggagt agtgtatgct atagggtttt gcaattctaa ttttggattt
     2041 aacacagaaa catcaaccaa aaaaaacccg tttctttcat aaacacctct attaattgaa
     2101 attactttgt tatcataagt tttaaaatct ggtaatatac acacaactct tgctttcttt
     2161 aaaagattat tattaagatt tgcaaagaca gcatagtaaa tgctatttaa ttctttaaca
     2221 atattccttg ctttctcaag attaagtgct ttctttaaga aaggtcccat atatggttca
     2281 aaaacacaag catctgcaga gaatcttgtt tttgcagagt ctccttgtat tattttatat
     2341 ctgcttgaca atttaaattc cttaattgcc caagaaacat tcttattgct tgcttctatc
     2401 atttctttac tgttgtctat tccaacaaca ttaaaatttt ttataagacc ttcaatcaaa
     2461 aaggtgccag taccacagaa aggatcaact attgtctttc cttttggttg gcctaaaaga
     2521 ttaatcatta ttgaaacaag tctaaaagaa gtattataca tctcctttat tactggccta
     2581 tttttgtcct taaaaatatt atctttagga ttaaaacaag atgtggtctt tgcaaaatag
     2641 aatttattat taaacttaac aacaaaaagc tctaggccat catttatctt ccaagaaaag
     2701 taattgtctg gatttgcaat gtaattaatg ttttcttttg atctcttctt tgaatgagta
     2761 actggttttt tataaactgc ttttgctctg tctgccttaa attcttgctt aagcaggtct
     2821 tctacaatct ctaattcatc ttctaaaata tcaattgttg aaattgtgta attaaatttc
     2881 tttgggctat caaaatttaa tttgtttatg atttccttat tagatttaat tttggaagtt
     2941 tctagataaa cctttgatac tctaacaaga ccaccaagtt cttttgtaag gccttcaaaa
     3001 ttatgttctt tattaaaatc aagaataata tatttttcag aattagcaat aacagaataa
     3061 gaaatattgt gtttatgcag ataaactgca atctctagtt ttgaaagatc tttgtctctt
     3121 ccaagaagga atagatacat gttttttcgt ttaatggtaa ttttttaaat aaaaataaaa
     3181 aagaaaaaag aaaaagtaaa aacttacttc ttctttgctg gagttgcagc ctttcctgca
     3241 ggagctgctg gagctgcacc ttttgctgga gttgcacctt ttgctggagt tgcacctttg
     3301 cctgctgctg ttgttgctgc tgctgttgct gctgcatctg ttgcctttgg tgtaacttct
     3361 tcttccttaa tctttgattt cttctttctt acagttccaa cagattttaa gttactctta
     3421 ttcttttcaa caataacttt catcttacat ggaaacttag aagccattct ttcaaataat
     3481 tctttaacca tcttgacatt tgcttcatca acaagaacac tatatatctt ttgacctttt
     3541 gttactcttg aagctctgcc aacaacttta ccaaaaggac actggctcat tcctgtttga
     3601 attctatctg cgtgtgcgcc ttgtgcctgt ttattttctc ttaagaaaaa ttggggataa
     3661 actcttactt tcatataata gtttacagca gttaatttct tgtttaattg tcttacaaca
     3721 ctttgtctta ctgcttcaat tgcattatct ctaatctgta ctttttcctt agttacataa
     3781 tgcacaacgt gagtataatt tttagtccct gtacccatat ggaactgtct tatctttaaa
     3841 cctggaacac caccaataaa gttcttttta tgaaccttta ctgcaattct ggtgtatgct
     3901 cttttattta ctcttggaca ataacaatgc cctggtctta atcccatctt gtttcacctt
     3961 tattaaaaaa atagataaaa ttgtcgaaat ttatctttat tgtatataaa tatatttata
     4021 tagaaacata tttaaataaa acattttaat aattttaaaa aaaactagaa tatagaaagg
     4081 aaaaagaggc ctttttgcag gtctcctttc tctttattac atttcttttg gggaaagaaa
     4141 agtttttggg gtaaaacacc tcaaaaatgt aatataagaa agaaaagaac agggacctgt
     4201 tctggtaaac agtgtgtttc tcttcttcct cttttctgcc ttgtatttta ttctttgtgg
     4261 aatttggtaa tagatttaac tgttatatct tatagtccta aaatctttat atatactacc
     4321 gggtggtttt tccaaaccta aactagaaaa agggtttgat tagcaaaata tacataataa
     4381 aatgtttgtt tttttgaggt ttttaactct tttttgcaaa tttaccaatt taacttgtcg
     4441 ccaatagaga agagcttttt gccatatttt gcaggaagtt ctataatata gcgacattta
     4501 tctcttggct ttgtcatctg ccaaggctta agcccaagct tgatatctat aatcttcttc
     4561 ttagaatcta aaaacacaac atttattgga aaaaacacaa aaaacatgtg tatgcttgca
     4621 ccaatatgcg tatctctttc aagatcaaat atcaggccat aatcaaagtt tttctctcta
     4681 gaaaacatta gaccctttgc tctactaaac acattgtctg caatcctgtg agttgctata
     4741 atattttttt tctgcgagat gtttctaatc atattaatca ttatatttta attatttgga
     4801 taacccaaag gttataacaa attcattgtc ttgctgtttt gtaatcttaa aatcataaaa
     4861 tgctggttca ttaacaagta ttgtataggt tttacaatct gttaaacaat ttgaatcaat
     4921 aaatgaaata ttaattctgg tagactctag aatatattta gaactaattg tctttaaagc
     4981 aatgatgtgc ctcatttcta cttctgtctt gttgtttttt atcatatatt ccattgtatc
     5041 aataattatt tcttctgcat ctggcttagg aataaacgtc tgaaataaaa cgatagtaac
     5101 aacaaataaa ataaaaacat attttataaa atttaaacct ctatcttttt ttataatata
     5161 cattacatac gaggtaaatc cccctaaaag taaagcaata tacaagataa aattatcaaa
     5221 aaaagttcta gtaattattt cctctgttga ataccacaaa aaattactac caataatgtg
     5281 gtctatatac aataatacaa aaataaagat aaaataaaac aaaacagata aataaaacaa
     5341 attagaaaaa tatattttat tcttagactc gcttctaaaa agaagtaaat ctttagatac
     5401 gttctcaagt attttatcat aatcaattgt tgattttttt aaaacatcta ttattattag
     5461 agtgtgctca atcacagaac tgttaatgtt tttatttatg gaatataatg attcttcaaa
     5521 gctttcaccc caagaaactc tatttgaaag cctctttatt tctggattaa tattgccgta
     5581 aaaattatat tctgacagat tagataatgc caaagataag tttcttgttt ttttatactc
     5641 ttttgataaa tcatacaaaa aataattgaa ttcggtttct acagattttc ttttttcggt
     5701 ttttatgtag tattctatta aaaaccctaa gccccctaca aaaatcccag aaataaaaac
     5761 tataaaatag atattgcttg ctgcaaaata tttgttagct atggctataa ttaaaagacc
     5821 acaaacaatt cctgctaatt ctaatataga atattcatta ttataaaaaa aaatatttgc
     5881 tttcttaaaa aaatcatttt cttgaggatt tataatcatt cacctattta tgtaatatta
     5941 aaaccaatat aatttcaact attggaaaag caatagctat aataaataat atatttttta
     6001 tagagtccct ataaaaaggc attaaaaaaa cactaaatat caatatgaaa attaagaaaa
     6061 acaaaaatat gtttatttca aaaagcaaag taattttgct tcttttcctt tcttctttct
     6121 cttttcttaa ttgatctgta gattccagat aatctgaaat tacattagat agtgttgttt
     6181 tattttctaa aacatctatt aattttgaga aaaaattata ttcttgctct gaataagtgc
     6241 tattacaaat ttgttttaat gttcccttta agttgttatt taaatcagac attgctaata
     6301 ttttttttga tttttcagtt aatagtttat tacttccaat tttttgaaat aaatataaaa
     6361 catctttaac gctcatcttt tgatcttgta aaaccgataa atgccttata ctaatttcta
     6421 attcgtgctc taattccttt ttgtttgatg taattaaata atgtggatag cgatataaag
     6481 tcataaaaat aaaacctgca aaagtaaatg caataataat tgttaaaaca aacgtaaaaa
     6541 ccgaaaagag gatgttcaat ttaagcatta caaaaatcat aaaaaattct aaacaaatgc
     6601 ccacaagaaa agacaagaat aatgtattag atatataggt ttgaacatct tcttctatgt
     6661 tagttataac aagcattttt gttagttctt taaacttagg cgcgatgctt ttagaaatat
     6721 ctgaaaaaaa aaaattagat atttttgata gtgttttacc aaacattatt tatttctcct
     6781 gtttttacta ctccaaaata tttaattttt ttaataattg ctttttgtta ttgtaataat
     6841 cgttaactat attattaaaa tcagtaccat ctgttatccc cgaatcgact aaccatcgga
     6901 gtaacttttt gtgattaaaa aactctgatg tgcttattgc tgaggactct agattaatct
     6961 taaaatcgga atcctttttt gaataataga ttacgtcatt atcttttccg ttgcttattt
     7021 ctaatatctt aataatttgc atttgttcaa tattgtttat ttttgttcgt tctagactta
     7081 ttataatcgc tttcctatta attgcttttg ccttttctga atattttggc agcaactcct
     7141 ctggcagata aaccattgaa aaatcatacc atttaatatc aaataaatct tcagagaaat
     7201 tatctaagat taaataatct gatctttgag aaagcgtttg agaaattatg gtctttttat
     7261 ttgatgcaat gttatctagt aataatttct taatccaatt cttttgtggc agttttatat
     7321 aatcaaagga ctgtatgcta gtgattgttt tatctggata aaattgagcc aatgcagaaa
     7381 taaacaaatt tgcatctcct ccggttatta atatttgaaa atcgtcatcc attgcagtcc
     7441 aaatataaga atatatatcg gttatgccta ttcctaaatt tataaggctt acgggagtta
     7501 acggttcttc taaatatttt ttaataataa atgaagaccc tctattgctt atgtcgccta
     7561 cagaatatag gccttctact ttgtagccat taggcaaata tccatttatt attggctgag
     7621 cagaattaac ttgtaacccc attatttttg ttaaagaaag aacaaattga tttagtttaa
     7681 atagattttc aaatttaaga tttgtctcaa ttgccccata ctttaagtgg tatacgatta
     7741 ttggcatatt ctcgccagaa caactaatct ctattatatt tttatcttct aaaatggtcg
     7801 acaaaaaacc aaaacctaaa aagtcccttt taataaaatt aaaatataat tttttttcta
     7861 aatctgttaa attataagac aatttgtcaa tagttaaatc aaatactttc ttaatatagt
     7921 tatttagatt ttgtttatca cctttaaagg aggtgtaatt tgaatcaata gaatccaaga
     7981 aaatcttttt aatttcgaca tatgtttctt cttgagattg attaagttct ggctgaatta
     8041 cactatatac taatccctga gacatcatgt aatttatatt cacaaaagta aattcatcga
     8101 tattatattt ttcggagata tttattttat cggtttcaga tatttgaggt ttgacactat
     8161 aaactttagt tatagaactg gattgttgaa cacatggttt gtctattgtt tctttggtaa
     8221 ttctagaaat tgcggtttta gataatgttc tttcttctgc atcttttggc aacacataat
     8281 caaaggatgt tttttgcgaa actttggttt gatttggttt ttcttcaatt agagattttt
     8341 cttttatttc attattatca aaagaaaggt ctggaacaac aatatcttca atataattgt
     8401 caatatttaa ctttggcttc tctgattttg ctttctgctg ttctttttta taattactaa
     8461 tattattatc gatattttta ttggtattta ttttgccatt atccaaaata aatgttgact
     8521 cccttatttt ttttgttgga ggaatttgct cgtaagttga aaatccttta tttactgctt
     8581 ctgaaatatt tgtatcatct ttaattgaaa aaatttctgg gacttctatt tcttcaagta
     8641 aagcataaat gttattagta tttttttgat cttctttatt ctgttgttct ccattatatt
     8701 gtgattcttc ttttttaatt tttttctggt ttataacttt tgtttctatt gcatctagtt
     8761 ttgcataaag atgagagacg cctgtatctt ttattgcaat ctttttaatt ttggggtcat
     8821 catctaagaa actaattgat tcgaaaaatg gttgttcatc ctctacttta tctgtttttt
     8881 cttctaatgc tgtcaataaa aaatccactt ccggagtatc tttctctact acattttcta
     8941 aattagtaat agtaattctt tctgcaatat cttgtaattt attagatatc acttctagtc
     9001 ttttcttttc aacttccaat cttaatgcat tttcaacata ttcccttgga gtaattgttt
     9061 caattgttgg ataattagat tctgaattat tttcatgtgt ttttgtttct attttaaaat
     9121 ttctaaattc ttcttttttt tccgaaacat tgtgtaattt taactttgga agactaccta
     9181 ctttgttctg atagctatct ggaggagtat tatctataga aatattaaat ttttgtgtga
     9241 gtgttttttc tatttttttt ggatcgctat tatcattaat cctagacaac ttttttagtt
     9301 ctaccatatc gataactaca taaatatatt tatagcaaat aataagggca ttatatttat
     9361 aaatatattt taatgcctaa attctaaata aaagaggtgg cgaaattacc taagagtgaa
     9421 agcacattaa aataggtcaa tacggaaaca atagcataag caattgttag tattaataca
     9481 aaaggaacta atggagatac ccatatttta tctatgcgat gaattttaga aaacttcttt
     9541 aattcatata tttgctgttc tggagaacaa tttggtttta ttgttgtttt agaaataact
     9601 ttattattta ccaatttata ttcttctaaa ggggtataaa aattagttaa tttatttatt
     9661 gcttttggaa agccaaagct cattaaaatt aagctttctc ctaatggctt tttagggaaa
     9721 ttgcctttaa ttgtattgta aattaaaaac aaaattggga aaaacaacgt taaaaccgta
     9781 gacattaaaa agactagccc tgtaaataaa acagaaatac cactaataaa actcattgtc
     9841 attaatgcaa taaggtcccc ttccccaata tatgcataaa ctgtaaattt tcctaataca
     9901 tagatgatgg ttaagaatgc aaaagatatt gcgatctgta aaatatacat atataaaaaa
     9961 cctttaatta aaccaaaaaa aatataatat gcaaaaccaa ttgcagaaat aactaataat
    10021 ggtacaatat ttatttctct gtattttaaa tcttgatatc ccaaataaat aaaacaaatt
    10081 aaacctatta aaaacatagg gaaaaatata atgctcatgc tcatctcttt tcttcttttt
    10141 ctttaattgg cttcttttct tttatttttt ttggtttttt cgtttgaatt gctgttttag
    10201 tcaaatgaat taaatcatca tatgcttttc tttttgttat ccttgcagtt gatctttcaa
    10261 acaattgatc agtgtatttg cctggtgggc caaaagaaac aatttgcttt tttaaaatct
    10321 tatttagtat tgcttgattt tgtaaagtgt tatttgtttc atcaattatt gattttggta
    10381 aatctatagt caaaactcca ctaccaattc tgtccacata gtttgtacca gagccagcag
    10441 taacaaaatt ggccatttgc gctaacttaa tggttgctga aaatttataa actaaatcgt
    10501 agcttttctg gtcaatcttt tgatttaacc tatctgctgt ttttgaaata ccggcaatat
    10561 taaaggcttt gtgtttttgt acataccata agaagtccct aagggttttt ataaaaaatg
    10621 aatcatacat ataataagta atgtagccca tcatgccatt atagccttcg acaccaacat
    10681 tcttttcttt tataaacttc tctaattttg ataatcccat taaatagctt gctgagttat
    10741 ttatcaaacc aaaagggtgg gcaatcacaa aaaggtaatc cttcctctca cacatatcat
    10801 agattcttct catcgaaaca ttctcttcaa aaaaaacatt ataactatag atgtcttcgt
    10861 ttggggcata aactaaaact tctccctgat ctgtagaaat ggttattccc ggaatcaaga
    10921 tgtttgcttt tggtttatct tttaacaaat caaaatatgc tttctttgga tttttctttg
    10981 cgttttcagt gattgcagtt cctagaagat tattcttgtt agcaaactgc cagtatcttt
    11041 tacttgactg ctttcttttt agaaactgcg tatttaaatc taagttatat gacatatttt
    11101 tcttttattt ttttaaattt actcttgcat cgactatgta gatttctttt tctgttagaa
    11161 ttactggatt tagatcaatc tcttgaagat gcttgtcttt gctcaactcg cagacttttt
    11221 ctagtaagga gattagcttt gagacaggat agttctttcc tcttgcagaa aagtacctac
    11281 ttacttttgt tgtataaacc ataacttcta tttctttttt tgttattggt aaaagtctta
    11341 ttgtagtgtc atcttgtaat tcagtgtaga taccaccttg gccaaagact ataacttttc
    11401 caaattgagc atcattgttt attcctatca ataactcaag gcctttaact tgcttttgaa
    11461 taattacttt gccttttact ttctcttttt ttagaacttc ttgaagcttt tcaaaggcat
    11521 ctatgaacgc ttcttttgtg taaatttctg tgtaaactgc tttactttca gttttatgta
    11581 caagagaaga actaagttta agtacatatg gaaaatccga aaatctttct atatcttttt
    11641 ttaaaagttc tttagattct aaaattgcaa tatttttttg ttttagatat ttaaaagaat
    11701 cctctaagtt aagccaagcc atttttttca cttcttttta taaaaataat gtttaaaaac
    11761 ccatatatac tataaatata tatattataa atatatctaa attcttataa aagtttagat
    11821 tctagaaata gaacacaggt tttaaaatca ttgtgtttta ctattttatt attatattta
    11881 aaatgtatct aattatagac acatcttaca aaaaaaaata caaaaataaa ggagagaaat
    11941 aaaatgaaat tacattgcct aggggcagga caagaagttg ggagaagcgc cctattatta
    12001 aaaggcaaaa atgaaaacat attattggat tatggtttaa aacttaatcc aacaacacat
    12061 gtaaaagcag atatggaact accagcaaag actgagcacc ctaaagctat aaaagaacca
    12121 ttagctggaa taattttaag ccatgctcat cttgaccatt ctggagcaat accgtcactt
    12181 tacaaagaag caaatgttcc attgtttcta acagaagcaa ctttagaact gtctaatctt
    12241 ttatggaaag acaccttaaa gattgcaaag ttaactggag aagattgtgg atttaagcct
    12301 caacaggttg atgacactaa caactctgca ttctatattg attatagagt tccagtacag
    12361 atttcgccaa acataaagat aactttcttt gatgctggtc acattgttgg aagcgttatg
    12421 gctcttatag aaatggataa gaaaaaaata ctttacaccg gagactttag agcaacagaa
    12481 agccagatgt ttagagggta tgatagaaaa cttccacaag ttgatatttt aataacagaa
    12541 acgacctatg ctgacgctcc acacaagaac agaaaagaac aagaaaaatt attggttgat
    12601 gagattagag aaacgcttga caatagagga attgttattc tccctgcgtt tgctattgaa
    12661 agatctcaag aacttgtaag tattcttgat agatataata ttaaggttcc agtctatctt
    12721 gatggcatgg caaaaaaagc aacaactatt tttgcacaat accctaatca gtttgtggag
    12781 tatgcaagat tcaaaaaagc agttgacaga attgaattta ttaaaaacca aaagaagaga
    12841 gaaaaaattg ttgccacaaa caccccttgt attattataa caactgccgg aatgctagaa
    12901 ggcgggcctg ttttaggtta tattagagac ttaggagatg acccgagaaa caaaataatt
    12961 ctaacaggat atcaagtaga aggcacaaat ggcagaagac ttttcgataa aggaaaactt
    13021 tatatcgacg gtaagattta tagcccaaga gcaaaagtaa caaaacacag cttttctgca
    13081 cacgcagata aattagaatt acttgagctt gttaaaaaag taaacccaaa gaaagtaatc
    13141 tgtattcacg gagacaaaga tgtcactttt gatttcaaaa atgaacttaa aaagctcgga
    13201 attaatgcag aaaatcctgc tgttggcgat attgttgatt tagattagag gaaaataaaa
    13261 acatgaaaag attttgccca aaatgtggaa aaaccgttcc tgaagaaaga atgattaata
    13321 atttttgttt gaattgttat atcgaagatc accaggtaat cacagcgccc acatttgaaa
    13381 tcacatattg cccaaaatgt aaaaaactaa gatatgctcg aaaaatcttt gataatgtgg
    13441 aaaatctaga aaaagaacta gtaaaacaca taagaatcaa agaccttgaa caggcaaaag
    13501 caaatgtgac tttggtttta gattttgata agcacgaata ttatgttaaa gttcttgtaa
    13561 aagctttgat tgctaacacc atacaagaga ttgcaatgga aaaagatgtt tctttaagag
    13621 ttgataattg cgagaactgt ctaaagcttt ctagcaacta ttacactgca atattacaat
    13681 taagatttga tacaaaagaa ctacttaaag aactacagcc tgttctagtt aatcaaattg
    13741 aaaaccaagt taagactatc aacaatgcag actctaataa aaataaacct ttttttatag
    13801 ctaaaactat tgaacaaaaa actggaatag atatttactt taatgattca gatcatgcag
    13861 aaaaggttag aaaaactcta ctttataata agcacgtaaa gacctggcaa aagacaagaa
    13921 gcctaattac tgcggacaaa gacgggaaaa ggaaataccg ccacacaatc tgcttacatt
    13981 atggagtatt accaacagaa agtggagcaa aagaactaga agaaattgaa aattaaaata
    14041 gttctttaat tattttaatc ttctctcgtt ttgcaacaat taatattttt tggccttctt
    14101 ctaatttatc tttttccttg taaacataaa attcagaatt cttaaaatat ccaataatat
    14161 tacagccttc aggaagtaac tcctttagtt tggatatgtg taatcctatg tgttttgcat
    14221 cttttttcac tagtgtctct acaattgctg catcacccat actataaaac gatagttcta
    14281 agatatcttt atcagtaatt aattgggcta tatatcccgc agccgcataa tctggctgaa
    14341 ttacataatc aatacccagt ttatcaaaaa catctttttg atagccaatt tttcttaata
    14401 ttactgcaac tgttttaacc cctgcttgtt tagcaatcag agcaataatt aaattagttt
    14461 catcatcatc agttaatgct agaaaaacat ccgcatgatc aattcctgct ttttttaaag
    14521 tgtttatgtt tgtggcttca ccattaattg taaccacatc aaattcgtaa gctatggctt
    14581 ttgaaattgc aggatctttt tcaataacta ctacttcgtt ttcttcagca tctaataaat
    14641 caattaaatg ctttgaaact gcgcttcttc ctactattat aacatacatt ttatttcacc
    14701 tttaattttt tttgcaataa catacccatt aatccaaata ttggaataat ttctagtctt
    14761 ccaatccaca tatttaatat taatgcgatc tttggaacaa ttggcattgt gtgacttgta
    14821 atcccagtgg ttataccaac attaccttgt gctgaaacta cctcaaaaat actatgcgat
    14881 attggataat tatatgcaac taatattaat actgtaatac caattaatat aatataacta
    14941 ataatgaaaa aatataagaa cttaatgtcc tcgttatcca ctgcttctcc attgtgtttt
    15001 tttggtaagc ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcagac
    15121 attataaaag tagaagcact aaacaaatcg actcctgcta taaataatgc gattatccct
    15181 attagtgtta agcctatata taaaaatcca aattctctcg ctgttttttt tgggttttga
    15241 tgtataaaat cgtgtccttc agccttaact attttcttgg tattaagtat ttccgaattg
    15301 attaatccaa aataagcaat aaaaataata ccaatacccc caatccaagc aacaaatgct
    15361 ctccaaaaat ttatgctttt taaaattgga atgtttggca gagttgttag ccctgttgtt
    15421 gttattaaag acatcatttc aaaaaaagaa tctaaaaaag aataacctaa aaacacaagt
    15481 ggcaaagacg ctaatgccac aaatattaac caaacaccac aaactataac taaatagtga
    15541 attaaattta aatgaattat ttcttttttt ttaattaatt taacccaaat tagaccaatt
    15601 aaaaaagaaa taataaatgt tgctaaataa ccaaatataa caacattccc ttctttaaag
    15661 attatagaaa atattaaagg tatcaaaaac aggaaactac tataatagat aattaaacct
    15721 attgttctaa atatctcatt aaaataccat cttgtgtaat acattttttt tcttattttt
    15781 agaatcttat agtaaatagt aactaaaata tatttaaatg tatttgcttt tattagttta
    15841 aatttctatt tttaaaaatt gaactaataa cattaaaatg ctaataaaaa ataaagaaaa
    15901 ataataaagc caggattttt ttgttttata taaataataa aaaattgcaa ttccaaagat
    15961 taaacctagt agataaacta ttgctggtgt ttgataaaaa aatatcccaa tggtgacaag
    16021 cgctgttgct aaaggaacta ccataaatgt tgctttccag aaatctggta attttgattt
    16081 gtgtaaaatc aaccaagaaa ttaaaaaaat aaatattaaa aataatatgc gcattattcc
    16141 aggaataatt atatagggag aatgctcatt aaattgtata aataacccaa acagcctact
    16201 taataaccct tgaaaaaccg cagctaatag gaaggtcaaa attagaagaa cataaccaat
    16261 aacaataatt ccaataccag atattatctt ttctagaatc ggatttttaa ataattttga
    16321 gctcataatt ttctcgtttt taaaatattt taacgcaggt cgggcgggat tcgaacccgc
    16381 gatcccaagc ttaggaggct tgtgcactat ccaaactatg ctaccggcct tttttctaaa
    16441 aagaataacc tttttaaata ctaagttttt ctatatataa tatatttatg agagtgttta
    16501 aaaagattaa ggcaatgcat aaaacatgtc taagaaaata ttaataatgc ttggaccagc
    16561 aggaagtgga aaaagcacac aggacaaact gatagaaaat gaatttggat ataaaccttt
    16621 tatcatggga gacatactaa gagaagaagt taagaaagga accacccttg gtaaaagaat
    16681 acatgctttg ataaaagatg gtcacttagt aactttagaa atggcaagcg acctaatctt
    16741 tgataaagta aaaaagtcta aagctaaaaa aataataatg gatggattcc ctagagaata
    16801 ttatcaatca attgtttttg attattttat aaaaacaaac aactataagt tgatgggcgt
    16861 tatacatctt aacctttcta gaaacgaaag cataaagaga ttactattaa gaaaacgaaa
    16921 cgacgatgaa ttaaaggcca ttaaccaaag acttgatgat tatcagggca ttacaataaa
    16981 agcaatagac agatacaaaa aagaaaataa aatcatagag gtaaatggag aacaaaaaat
    17041 agattgcgtt ttcaaagaaa taaaaacaaa attaaataaa attaaaggta tataaaacat
    17101 gattcaagat cccagaatta tagacaatat aaagaaatta aaagaattgg gcatgagtaa
    17161 tgatgacata aaagacaatc ttataaagat ggggctttcc tctgatgatt gtgatgaact
    17221 aattgcggct tcaaatgaag acgaaaataa aaatcgaaaa cttcaagaaa aagaacaaaa
    17281 gaaagaaaaa ataaaaaaag aagaaatctt tagtgaacca aaaaaaaatg aacccgcaga
    17341 aattccagaa actctatttc aagaagataa taaaattgat gcattaaaag aagaagtttc
    17401 ttttactatt aaagacgact tttctgaaat tccggattta actagtggat tagatgttga
    17461 ccaattaaat aatagtgata cagacattaa ttttaatgcc cctgacttat ccagagaaga
    17521 agaaaaaata aaatttgaaa aaccaaaaaa taaagacttt gatattatta aaagcataga
    17581 tttagataaa ataaacgccg ctaaattaat tgatacaacg ccttctgaaa cgttaaaaga
    17641 attggatgca aattcaaata tttggggatc tggattagca accacattaa acgcaagatt
    17701 aaatgaaata gatgaaaaac aagaaaattt tgaaaagctg cttaagaaaa aaatggaaga
    17761 cgaagtaaaa aaatttaaac aaatccaaga aacttcaaaa caattacttg ctcaaaaaat
    17821 tgatgaagaa cttgcaagta gaactgccaa cattaattct caattaacaa aacaacttgc
    17881 acagataaag gtagaattaa taaaaattaa taaaaaatca gaccagatga ctactggcaa
    17941 acaagaagtt gaagatctta taaaaaggtt aaaagaaata caaacacaag ttttagaaaa
    18001 caataataaa tcacaagaaa atataaacag aatggtgttg tcaacctctg taaagttaaa
    18061 cactaagatc aaagaaataa atgacatctt agctttgcaa tccaaaatta cacaaggact
    18121 aataaaaaat acacaaacag caataactaa tgaaataaaa aaattcaacc aatttaaaga
    18181 agaacttgaa aataaaatca accctcagca gatgtatgat aaacttaatc aattagaaac
    18241 atttaaacag caattagcaa gcagatatga aacccgtttt gaaaacgtaa aaagagaatt
    18301 tttaattaaa gcaaaagatg catttaaaga agaaattgaa acccaactaa aagaattaaa
    18361 aaatattaaa gaaacaattg tagctaaaac tgaccctaaa ttgattagtg acaaattaga
    18421 agaacttgaa tctttccaaa accatttaat ttctgcaata gatgaaaaaa tatctcaatc
    18481 tcttaagatt tatcaatctg caatgactca ggacatctct tctaaggcaa aaaccattga
    18541 tgaagaagtt gttaaagttg aaaatgcaat cgtaaaatta ggagacgcag aagataagat
    18601 aaaagaatta gatcagttta aagaccaatt tatcgcaata attgataaaa atatcgaaag
    18661 aatgaatgct acttatagta aattcgaaca gaaactaaaa gaaatagaag ataaacaaaa
    18721 actgatgata taatcagttt ctctttattt taaaaattcc taatataaaa aacagcatta
    18781 ttaataaaca aataaagatt ataccccccc acactgaatt tcggagtgtt aaaaatccta
    18841 ataactttga ttggctctta tctttttcta catcttctgt taattttagc agaattggtt
    18901 gttctaagac ttctccgtta tttgatacaa atcttaattc tgcctcaata tcttcttttg
    18961 gaatatctcc aatattaatt tttgcagaaa gtgtttttgt ttctccagga tttagggtta
    19021 ttgtttgatc aacaactaaa tcaatatttt gcgggatatt agtcaattct atcttattaa
    19081 ttgttatttt attgccttta tttctcaagg taatttcaaa ttcttttgaa ctgtttattt
    19141 caacagaaag tgtttttgga tatgccaata attctatatt tagtggctct gatattttta
    19201 caacattaat tatataatct aattcttcag aaaagttgtt tgatgtccca tttattttaa
    19261 catttaatga agataagcct agttgtgtaa aatcacctgc tgaaacatat attgttacat
    19321 attcgttgtt gtgtttatca acggttattc ttgaatcttg tgttgttgct tctaaatcgt
    19381 tatttggatt aagttctatt gttatatttt cttttacatc tcctgtattt ttaagtaaaa
    19441 tcataatttg tatggtttta cctattgcaa gatttaaatt tgattcactt aaattctcta
    19501 ttttaaaatc aaattttgga tttactacta atttcacgtt ttttgaaatt gacttaagac
    19561 catcaaaaaa atttaatttt aggttgtaag taccagaaat aatcttatct gaaactgtaa
    19621 aggtaaaaac aatatttttg ctttgatttg ggtctaaatt aaattgacga ctagaaatat
    19681 ctactttgat ttctgaatta acattgtctt cagaaatata gaatgttcta tttgtattag
    19741 aaatattttt taaagtataa gacaacgttt tactttgccc tttacttatg ttttgagttg
    19801 ttgatgcaga accagatact gtaaaatcat tatttggctc tgggcaatcc tctgcataaa
    19861 gagaaaattc tttaattatt tttatctctt cgttttctat tcttaaggtt aaatcaaatt
    19921 cttctccatc tgttgcatta ttagaaacaa ttactgaata atctatttct ttttcgtctc
    19981 ttaaatatac tggattagaa tattctgaac ttattactaa ttttggactg cttaaataaa
    20041 taccgtataa tccatcacta tagtcgtttt ctatatctaa agttattgta tttgttctgc
    20101 caatacatac atttgtggtt gagagatcca ctgaaaactc tccatcttct tcgtcttctg
    20161 taacttctat atctgttcta aattcctctg tatcttgatc ttctgaattt agtaatttaa
    20221 tataaactgt tactttcgaa tccgattcat caagagcaga aacatagaaa gaatctgtta
    20281 attcggaatt tcctgggaga gtatatctct tagaataatt acattctaat tcatcttcat
    20341 cttcgcagtc tgcccagact cttatttttg caacaacggg gttgttattt actatagtaa
    20401 aaccaatctt tgtgctagtg tcttgcatta aagaatatat tcctttatca aatattaaat
    20461 caaggttacc aatttctggt tcatttgttt ctgcaaaaac tgcttttgca gtaaataaag
    20521 acagtaaaat aaatattcca ataacgagat atttaaattt attatgactt gattttttat
    20581 tatatttttg aaaatcattt aattttgtct gcatatactt tcctctttta tttcctatat
    20641 acatatttaa tatattttgt tgagaaagat aaaattacaa ttattaaaat aataattaag
    20701 attatttgaa aaattattga attaaaaata gttgttagat ttatactttt tgtaaaagaa
    20761 actaaccctg taaaaacaga aataacctgt actttggatt tatctgtttc tttttttgga
    20821 agctcgaggg ttacataacc ataaacccca ttacatctat attctatttt tgtttctttt
    20881 aagtctgttt taaaaactct aaccattttt gtttctacat cacttggaac aattacaacg
    20941 cctattggat attctagatt tatatctttt attcctatta catctagttc ttgatctttt
    21001 ttgcttgaat tattaaaaaa caaatctaat ctatagtttc ctgattcttt aacataaact
    21061 atagtgtgtt gtaaatcaat tggacaactt ctatttgctt tctgaataat ctcaaattct
    21121 attttcttag aattttgtaa atacattaac tctatacttg ctttataggt tgaatctaaa
    21181 acattttctt gaggagaaat aattaaagta acttctttag attcacttga tttaagtgtg
    21241 attttgtctt cagaaaaata agactcgtaa ggagaagtat atgacactag agaataggtt
    21301 tgttctgtag aatttggatt ataaacataa agagtaacat agatgggtgt atcaaccaca
    21361 gttacttttt ttggaaaaga aactgttggt gtgttctctg cataaattat ttgcaaagaa
    21421 aataataaag taatcattaa aactaaaaat acattttttt taatcatatt tttctctttt
    21481 tttgagttat taacttatta cagttatact atataatata tttatatgga gaaatgctta
    21541 taaacttgat taatttaaaa aaaacagaaa aaaagctcta gaaacagctt atcttgccat
    21601 atctaggcct ataactgaag aattaccttt tagagttacg ccaataacct ggtttgcacc
    21661 agaaacaatt gtactgttgt gggtaataca tattactgct actgattttg acaattcctt
    21721 gatataattt agaaggctct ctgagttttg atcatcaagc gcagcatcta cttcatcaag
    21781 aatatagaat ggagattttt tataggcatg tactgcaaaa ataacagcaa gagctgttac
    21841 ggttttttct ccaccagaga taatgtctat gtttttggtt tggccgtttt ttgttatgtt
    21901 aatcattagc tgagcggtta aaggatcatt acctgaaagc tctaaagacc cttttaagga
    21961 cttagacatc tttgatatga catttttaaa ttcagcatta actttattga aacaatccat
    22021 aaagattgtt gtttttttca tattgatttc atttagcatt gcttcaactt gtgctttttc
    22081 tgcaacaatg atatcaaatt tctcaataat ctctgcatat tcctttgcaa gcttttcata
    22141 ggaatcgatt gcattaaaat tgatattccc aagagcgttc ttgtctcttt ttattgccct
    22201 tagctttgag taaagctgct ctattgtaaa ctcaacggtc tcgtattttt ctttaacctc
    22261 aagcatttta agactttgat ctgcttgaag aactttgctt tttaaagtat taatatttaa
    22321 tcttgcatca ttaatttcaa gattgatttc tgaaatagaa acatcaatct cgtgtatctg
    22381 ctgattaatc aaagaagact cttgagagac ttttgatttc ttttgaaaca tgtcctgctt
    22441 tttctggtct tcggtttgga actttttgtt tatttctttt atcttgttgt caatctctaa
    22501 gatttggtca ctataattat tcatttgagt tttcagttct ttaagtttag aattatttat
    22561 ttctataaga ctagatttac tatctatttt ttcctttact gttttttctt ctgcattctt
    22621 agaaattagg ctttgttcta ggatgtttgt ttctgagact aaagaatcat attcgtctct
    22681 taaggttgtc tgagcaccaa gatttaaact ttttaactta tcttctatct cttgcttttc
    22741 ttttcttaaa gtaacaagta tattatcaag ggcggaggtt tcgtctttaa tctgtccaag
    22801 attttgtgta attgtcttaa tggttgttgt acttgtactt gctgatttta tattgtctgt
    22861 tttttcaata atctcatcaa gagcagcaat attagtatta agctttactt gtaaatctgt
    22921 tagttcttta tttttttcga aatgcccttt attacttgtt tcgtggcatt taataatctc
    22981 taaaagcttg gattttgctt tttctagctc tttttctgtt gctgaaattt tgctttcaag
    23041 ctcttttggg tctttgatca atatcttctt ataacagaag ccatcaaagt aaaagccatc
    23101 aatgctttgt gcctttgtct ttgtacctgc gtgttctgaa aagatctttg atagttcttt
    23161 ttcagatata tcaagtgttg taagaactac aaaattaata tttgtgtgtt ctccgatttc
    23221 tttaaggatt acagggatat ctttagagtc ctttaaaatt acataattag aataaataga
    23281 tttttgagat ttctcaagag taacaaattc atctaaaaaa ccaataaaac ttttgtgttt
    23341 ttttagattt gcttttaact tttctctttt gtcaagagct aaagaactaa aagatagatc
    23401 ttttctaaaa ccatttagtt ctatggtttt ttgagatatt actttttgaa gatcttcttt
    23461 ttctttttct tcgattttta atttttggaa taattttgag atttcgcttt ctagagttgc
    23521 tatttctttt ctttgttttg agataattcc ctctatttct ttttttcttt caagtagtgc
    23581 ctttgtctct gcttcttttt ccttgctttg cttttcaatt gtttcttttt gaaactcata
    23641 ggctgacatc tttgttttat ttgagaaaaa atcctcttgc ttttgggtta tgtctttgtt
    23701 tatacttgaa agtcttgtgt aaagctcttc ttgtatcttt ctaacaccct caaattcctt
    23761 agagtgctta tttatctgtt ctttaaggtt gtcttttgtt ttagatttaa gctttaattt
    23821 tccttcttgt tctttaatta ttgctttaac taattgctct tcttttttta gttctgaaag
    23881 ctcgccagaa agtctttggt tttcgttttc taagtattct atattttctt ttctaagatt
    23941 aagattattt tgaataatct ctttatttgc cttgttctct gaaagctctc taccaactgt
    24001 tgattgtact ttttctgaat gtgcaatcaa atcattatta atattctcta atttgttttc
    24061 atattctgat atcaatagca ttagttcttt tttcttctgg tttttaacat caatatcgct
    24121 aataagtttt gtaatttttg cttcttcaag aaatagctca ctattgtatt catctgtttg
    24181 cttattgtat agattataac tgtagatgtg ttctagacta tcaagctctt taaacttaag
    24241 cgctgcttct ctttctgcag acaattgttc aacatatggt tttctttcgt ttagtgctat
    24301 tgaaacttta tctagtcttt tatttacttt atcaagatta tcttgtgctt ctttttttcg
    24361 ttcatcaaat aaagagattc cagaaatgtc ttcaattatt tttcttctct cttctggatt
    24421 catgttaatt atttttgtaa cgtctccctg ctgaacagtg taatagccat cagcagtaat
    24481 tccggtttcc tctaaaaaag aagtgatctc gtttaaggct tttcttttgc catcaagtat
    24541 acaaacagac tttcctgatt cgtctatttc tctaataatg tctatgtctt taccttcgtg
    24601 gttaaaaaca actctaactc ttgctgttct gtctttagca gattggttaa caagattagc
    24661 ggtcttatca actcttagtc tttttagaga agtagaacca aaaccaaaga ttagagcatc
    24721 aataatatta ctttttcctg aaccgtttgg accaacaata acttgaaatc ctggtttaaa
    24781 aggtatagta gtatagtcaa aggatttgaa attctttaac acaagctctt ttatatgcat
    24841 ttttgattct ctttttattc tcttaggtag tttgatatat cattaatctt aatagtttta
    24901 gaacaaactt tatttaaatg atacgatttt gtttttggaa aactaacatt aattacttgc
    24961 tttccaagag cttttatttc ttctattgct ggaataaagt ctgcatcatt agaaacaagt
    25021 attgcaatat cgtagttatc cttgtaagca ttaacaacca tatcaactgc aagtttaaca
    25081 tcaacccctt tttctgattt cttaccatct ggtctttttt ctaatctacc aaaaaaaata
    25141 tccattacag aaatttctct taaactatca aaaaacagtt gttgcttttt atatgcttct
    25201 tcatcatcgt ctatatttaa taccgaatta taatatttaa ttactattaa atcatcattt
    25261 tctgagagaa aattacaaaa cttttcaaaa tcaattttgg ttttataaaa agaattcctt
    25321 aaagaataat agaaattgct tccatcaata aatatcatca ctcgctttat agaatcagac
    25381 atttaaatca cataaaagac cgcctgccat tgaaaatctt ttggattttc aacaacaggg
    25441 agctaatatt atatataggt aacgaaacgt ttttaaacat taaagttaga tatttgctca
    25501 cgatagaaat tactgccatc aataaatatc atcactcgtt ttatagaatc agacatttaa
    25561 atcacttaaa gaaatcctac ctttgtgaac ctaaaagatc cccaatggta gggagttaat
    25621 tataaatata ttatagcgat atttttttaa atattaaagt tgataaaatg gctattacaa
    25681 taaccgtttg atttttaaaa aataaggatt atttgcccat atttgatcta aaatcttata
    25741 ggaattagca aatttaaaat atcctaaata actagatatt gaggatttta ctttgaagaa
    25801 gttcttttcg taagttaaaa tctttagttt ctttataaat ttcttaatat ttcgttttct
    25861 taaaaacaca tattctgggt atattttata tcctacaaaa tcaactccgg tttttatttc
    25921 aaaaaggttg attttttgct ttggaatttc tagttttaac cgtttcttta aataatattt
    25981 tatgttcttt ttatagtaat ttaattgctt ttttgaatct tctaaaataa taaaatcatc
    26041 cacataccta taataatatt taatctttaa tttgtgtttt atatattgat ctaattcgtt
    26101 aagatatata tttgaaaata attgacttgt aaggttacca ataggaatcc ctttatcttc
    26161 tccaaaattc gaataatcac tatcaataat atcaaatatt aatttttttg tgttcttaca
    26221 tcttatcttt ttttcaatta cttgctttaa gataatttta tcaatacttg ggaaatattt
    26281 tttaacgtcg cattttaagc aataacctgt aaatctaaac tcgcgtgaaa ttgttttttt
    26341 aatgtctttt agagctttgt gtgtaccttt gttttttcta caagcataag ttgttgatat
    26401 cagtcgttta tcaaaaatcg gttcaatgac attaacaagt gcgtgatgaa ctactctatc
    26461 tttaaatggc aaatatttaa tttttctttg ttttggatca aaaactataa attcgcgata
    26521 ttttgattga gtgtatgtgt ttgctaatag ttcttcttga atcaataaca gatttttctc
    26581 taaattaagt gagaattcta atgtttcttt tgtttgtggt tttgcttttc ttgctttatt
    26641 gtaagctaaa actaaatttt taaaagatgt tatttttgac cataaattgt tatatgtttt
    26701 catgtttttt ctttttaaaa aaagattatt gtaatatccc tttttttaag tactaattac
    26761 aataactttt gcattttttc ggcatatttg gtatccattt tttaatacaa ggcaaaaata
    26821 cctgccagga atataccaaa tgtagttgat catttaaata caatctttgt ttgtttttaa
    26881 aaacaaataa tacaaacaaa ctctttgtaa acccacaagt aatgtgggta gacactacaa
    26941 cacaacgaaa cccgatgttg ttgttcgtgt tagacggatt gttgttcgcg ttcaagttca
    27001 gaactccggc gttcgtttcg ttgttccagt taccgccacg caaaaacggt tgtgttattt
    27061 agtatagcct aggtttaaaa cccaaaagaa gatagattga aatatttttt aaattaagtt
    27121 gtttttaata agccgccaag caatttacct atttcattaa tttttcttga tgcaatctca
    27181 tatttgttaa aatttataag atttagatct tttgaaagtc ttataaataa tcttagtttt
    27241 tctatttcta cacttgctat ttttaaagac tcttgtcttt caaaattata attataagaa
    27301 actatatttt caaagaaagc aataaaacat aatttgatct tttcgcctaa aacatacttt
    27361 tcagattttg ggaacttatt aattaaaggc caaagccaca aaaagaagtc atagctttcc
    27421 ttatatactt ttaaatcatc aagcatatat tcatcttttt tgaatttttc ttaaaactcc
    27481 agaaatcatt tttagttttt cttctaaagc atcataattt tcttttaaat cttcatcaga
    27541 tatcagttta ctattattta ataatattaa attagactgg gttatagaaa ttgcatttct
    27601 ggctttatta tactctattg ctttaagatt tgtatcataa ttattatagc cactggcaac
    27661 ataagacaat atctctattg cagttttatt tgtatcctca atcagaaaat aaaagtatac
    27721 atcattattt ttgttttcgc aatcatttaa aattgtcaat attttctcaa caacaggaaa
    27781 catatcttga tagattttaa aactagcaac tcctttatca gagatccaca ttatttttcc
    27841 caatttttaa attaattttt tttgcgcacg caaaaggctc gcggatactt ccgcgacctt
    27901 ttggaaacta ctgactaacg gacaaacgga cgatatgcca ggagccagtg gtccagaggt
    27961 cagtggtcag gcataacaac ggacctcggt cacgtcggct ctacaacaca acgaaacccg
    28021 aaggcgatgt tcgtggtaga cggctcgctg tacgcgtaca agtacagaac tccggcggtc
    28081 gtagcggcgc tccagtcgcc gccacgcaaa aacgcatggg gcgggccaga atccataaaa
    28141 aaactaacat aacctaatcc attatcttta ttataatctg aatttaataa aaacaattcc
    28201 ttatatccta tttttgtaaa atcaacataa tcaaaagcat cagaataatt ttcaaatgat
    28261 ccattatatt caaaaccacc tttccgttca tcacaatctc caaaatcact acctattaat
    28321 ttattacaat ccaaataaaa atcagaagtg gttctatcag tccaatctaa aacatttcct
    28381 gaaagatccc aaacttcact gccatttgtt agctttaaca ttgctagttg atttcttccg
    28441 gttcctgatt ctggatcagc tccatcatat gatacatttg ctacaacatc tcctgcattc
    28501 cctcttttta aagacccctg accaacagcc ccacttgacc aattgcttgg aacaacttca
    28561 atatttcgag caatagtcat ccattcttcg tttgttatta aatgaccccc aatggattca
    28621 cacgccaata ttgaatctgt ttgattaata tctgttagtg gataaccttc tgcactgctt
    28681 actattgttc tgctaggcac actatattta caacttgcat ttatgttatt ttgccaagtt
    28741 aaatataatt catttttaca actggcgtta gtatcgccga gtccatctcc attttcatct
    28801 actttcatct catatttggc aacacaaaaa ccttcggtgc cataaagata atttcctgga
    28861 actggaacaa agcctgtagg gcattgacta ggagctgggc ttctgtttgt gttttttaca
    28921 ttattaaagg ttaggtaact gccgtcttct agtaataaag caattgatat tttttcttcg
    28981 ttaataatcc ctaaaggaag catgaactcg cctgcgtggg tgatctcttt ttctggttct
    29041 agcgggatat agtcgttata gcctagaaga gaataaccta caactttaaa attaatatca
    29101 gtattgcttg gaggataata atcaaagata tacatgccgt tttgtgcgct tttaaagcgc
    29161 atgaaggatt gcttgtcgtt tagaacatta tagttgacaa gactatcagt ttttgaaacc
    29221 gaagaatttg taaagttttt tccccagttt aaaactatag ttacaagaat tactgcaact
    29281 actactaaaa gaattgttgc aactattgga gttatagctt ttgctttact atttttcatg
    29341 atctctgtgc ctctctattt atattattaa taacataaat aaataaaaga tatatatata
    29401 tatatatata tatatatata tatattgatt ttttagttat caaaagatat cgtttctttt
    29461 tatctcagtt ttaccataca tgtacttttg aaggactttt ggaatcttta ttgtgccttc
    29521 ctttgtctga tgattttcaa gtattgcaac aagcgccctt gaggttgcta ttgctgtgtc
    29581 gttaagtgta tgaacaaaat atttttcgcc ttgctttgtc tttgccttaa tgtttaatcg
    29641 gactgcttga gcacttgtaa gattactaca agacgcaact tcaatatact tgttgtttct
    29701 tggagaccac acttcaatgt ctgcgcttct atgttttaaa tccccaagat ctccagaaca
    29761 aatttccagc gttcttgttg gtagttccat tgaggtaaaa atcttaactg tatttttaat
    29821 taattcttca tatagttttt ctgattgctc tggtttacaa ataattatct gctcaacctt
    29881 atcaaactga tgtaatctaa ataaccctct ttcatcaagg ccatgactgc ctatttcttt
    29941 tctaaagcat gatgaaaaac caaaaatctt aattggtaat ctttcttcat caattgtttt
    30001 gtccaaaaac atagaaatga ttggatgttc agaggttgca atcatgtaaa ggtcttcacc
    30061 ttcaatcttg taagccattt ctttaaagaa agaaaagtcc acacttcctt cacaagcttt
    30121 tttgttaatc attaatggcg ttaaaacata agtaaagcct ttcttgttta ggaaatcctg
    30181 agcataattt attagtgctc tgtttaggag cgctaaatct cctttaagat aatagaaacc
    30241 ttttcctgaa acatttcttg aagcttcaaa gtctgcgaca tttagttttt ctgagagctc
    30301 tccgtgattg agaacttcaa agtctttttt ttctggttct ccaaatcttt taatttcttt
    30361 attttctgtt tcgtctttac caattggtgt ctttcttgac attatgtttg gtattttttt
    30421 ttgaatatca ataattcttt caaatagttc tttttgttta tcatctataa ctttaatctg
    30481 atcagggaat ttccttgctt ctgctaaaag gtcttctgtt ggtaatccct tttgcttgtt
    30541 ttctgcaatc ttgtctgtta cactatttct tttgtgtttt aagtcgtttg ccacttggat
    30601 atttgctctg tattcttcat caagtcttat aatttcatca actaaaggaa gaagaatatc
    30661 tttgcctttt ttttttatat tttctttaac tttcttagaa ttctctctaa tgaattttag
    30721 ttcaagcata attaatcaca cacaaataat aagaataaaa gaatataaaa agctttattt
    30781 tatcaaaaac agaaagaaaa gaagaactta ttccttcttc tcttctttac aatcgcaatc
    30841 cttaccttca caattggcaa tgttcttctt cacaatcgca gtcttcttct ccgcaatcac
    30901 aatgttcttc gtgttcttct ccacattctc cttgacaatg tgaacaatca ctatcgcaag
    30961 cctgtgataa cattttatcc atctcttctg cttcttcttt tgaaagaatc tctaatagtt
    31021 taattttaaa aattaaattt ttcccagcaa gtggatggtt cagtattgca tcaattgtag
    31081 tttcttcaat cttaataata tctattgcta gtggtcccat accagacatt acaataattt
    31141 cttttccttc ttgtaaatcg ctaattccgg ataaaagctc tttaggcaat tttgcaactt
    31201 ctgtgttttt ttcaccatag ccttcttttg gagaaacttt tatttctttt tcttcaccaa
    31261 cttccattcc aattattcca ttattaaacc caggaattac cattccaata cctactttaa
    31321 atttaagtgg ttctctacct ttactggaat caaaaacttg accatcactt aaagttcctt
    31381 cgtattcaac tgatacccaa tcattttctt taacactcat tttctttcag ctccttttct
    31441 aaaaatagaa tattatatat tatgagacaa atgtattata aaagaaatag ataaaaataa
    31501 aaaaaggacc tgcaaaggat tgcaggaatg tattatttat ttcttcattt tatacattat
    31561 gaaataaact attgctgtta aaatactaaa tattattgca gcgattgtcc aaataatttt
    31621 tatttcggtt tttaattttt tgttattagt aaatacatca tatattaccc atattgcaca
    31681 aattaatgca acaattccta ttatcgtatt taatacttcc atttttatta cccccgtttt
    31741 taaaaatact aatataattt actgaagaat aatgttttta aagggtttta ttcctttgtt
    31801 tttattaatt ttatatcttt aacaaataat aatttacaat tctgccatca tttatgctct
    31861 tgtctttctt tataaatttt gagccagatt ctaacatatt gtttatggaa tatttatttt
    31921 ctggatgtgc ggaatgtaaa aaatatttct tgcctttctt ttttaaatat tctattcttt
    31981 tttttattag aaacttctga ataccatagc ctctaaactt aggagaaacc gctgttgcga
    32041 ttaaaacact tgatttggat aattgttttt ctgtaaaatt atagattttt gcataaaacg
    32101 gctcggcttt tatttttatc cctgcccaag caaccaattg tttatcaaaa aacacaagca
    32161 ttatttttct gtcttttgaa atcatatttt taagtatttc ttttgtgtgc ctaaaataaa
    32221 aatcatggtt tacttgtcta tagatattaa tatcaaattt attgatgatg tcaatatctt
    32281 tctcagtagc aaaacgataa gtaaagattt tatcttttag ttttactttc atgtgatttc
    32341 aataatttct tttttagttt attataatta aattctttat ttaataattc ataataatat
    32401 gcatttctaa atttaccata gattacaact tcgtctctaa aataaccttg ttgtttaaac
    32461 ccaaactttg caatgtgctt ttggcttctt atgttgtcta ttaaaaccat agaattgatt
    32521 ttgtgtaatt tcaaatcttt aaacgcaagc ttaaagattt caagtcttgc ttctgccatt
    32581 aatccactac cccaatactt tcttgaaatc caacttccac tttcgcaatt cttgtttcta
    32641 aaatgaatat tatttaatga ttcaacgcca attatcttct tgtctttctt atgcaataat
    32701 ccaaagataa gtgttggtgt tgatttcttt aaattctttt tttgcatctt taataaattt
    32761 agtagcatca ctcattttgt aaggccaagg aattatccaa atttgtttag aaaatatttt
    32821 atcttgcatt gcttgataaa tatcattagc atcgcttagt tttaatggtc ttaagattag
    32881 gtgctttgtt tcaatcttat agtttttata ttttaaaaaa aattcttgat ttttcatatt
    32941 ttatataatt tacaaagcat ttaaaattat aagtgttatt actattaact atgattgcac
    33001 aaaacttgta tgataaacta gaaattgact ttatattaag acgtggatta actgatgatt
    33061 ggaaagagat tggaactgaa aagtatttat ctccaaactt taagaaaaga tctatgggaa
    33121 tagtctatga tttctcagaa gatataaaaa aagtatatac tgctgttttc cctgaagatt
    33181 ctgtaataaa gaaaatactt gcaaagaaag aaactgatgt actattgttt gtgcatcatc
    33241 caatgacgtg ggatattact aaaaaaccaa tatttacttc aatctcagaa aaggatttaa
    33301 aaatatttaa agaaagaaga atatctgtat ataatctcca tgttcctttg gataattttg
    33361 ggagatactc aacaacaaac acacttgcaa aagaacttgg ggttaaagtt gaaactcaat
    33421 ttgcgcctta ttttggggca agatgcggag ttattggaac aatagatact gataaagttt
    33481 cagatataat aaaaagattt gaaaaagcag ttggacataa aatcaaattt tacaaatacg
    33541 gtttagaaaa aatcaaagac aaaagaattg cacttgtagc aggtggtgga aataatcttg
    33601 atattctaaa gacaatacca aaaggcataa atctttatgt tacaggaatt actgtaaaga
    33661 acgaccattc ccaaaaagca cacgaatact taaaagaaaa gaaaatcaat cttcttggtg
    33721 gaacacacta ctcaacagaa aagtttgctt gtatagcaat ggttgaatac tttaagaaac
    33781 aagggttgcc atcagaattt attgaaggaa aaccagtgct ggaagatatt taattctctt
    33841 attaattata tctaattaaa tatcgaaaat atatataaat tatattgtaa tatatattta
    33901 tgatgattat gaataggtgt tttttattat tgtttttttt agtattgcta attggaaatg
    33961 tttatgcaat tacaattaat gaaataacaa taactcctga tctagatata tcttattttg
    34021 gaagatacca aattgatgca aatattacag agtattctgg aaacgacgtt aatcttttag
    34081 tttctgcaat caatggggat ggtggaacgt gttgggatta ttttgtggat ggtacttgct
    34141 caagcgaaac gccttcaact tactctatgg attataatga aacaactggt ttttggcact
    34201 atgatagagt atatcctgac accatctatc ctgaaattat atttattgat tctccaacaa
    34261 attggaataa cgctccctca tgggtaaatc tatggagaca aaattatcat atctttaaaa
    34321 tgtataatag ttttgaaatg gattcaaaca tgaatctatg gattgaattt aatactgaac
    34381 cagtaagcac aaataattca ggagatcttt ttgtttatgt tgtgggagct ggcgttccat
    34441 taagttattt tacaagcgat tggagagatg attcaaatac acaacttgtt ggaacgattt
    34501 caaggaatac ggcatttaac cacgatcaca caccttcaac acattatgta agacatcatc
    34561 tgattccaat aactgcaaac cctgatggta caattggaac aaatcatgtt aatatatctg
    34621 atggcaattt ctatattgtt ctttatcaag actccacagc aacgaataga ggttggaatc
    34681 ttgcatataa accaacaagt ctttgtgata acaccgacaa ttggtttaca gcaaacagaa
    34741 caggaggaaa ctggaaccta ccgattcata gatctggctg ttcagacgct cacattcatt
    34801 ttgcaagaag agaaacttac aatgatggag taaatttaga agtgactgct gttgatggag
    34861 aagacattac aactcttgat gtaaactatt attttgcaga actaccaaac cttgcaccaa
    34921 acgaaacttc ttttatcaca ccaattcctt tagcaacata cgacactaat tccatagaac
    34981 tttcttggaa tcctgcaact gatccaaata atgatgattt gacttatgat atcaattata
    35041 tggatagcga cggaaatata tttgtaattg ataacaattt tacagacaca aacatttctt
    35101 gggacgtttc tgcactagaa gatggaaatt atttaattct tggaagtgtt tgtgatactg
    35161 aaccactatg tacggacttt aatctaagtg attatttcta tattaatcgt tctgaaccaa
    35221 tctattctct ttcaacaatc acaattgcgt caaattattt ttatacagac tatgcaaaag
    35281 ttggaagcca aataaatttg tcttttattt ctacaggaac attatcagaa gacctaaatg
    35341 tgattttcta tgcaaatggt gtagaagtaa ctggagacat aaatgttaca aataatacaa
    35401 atgattacaa tgcaacttat gttatacagg aagaagacga tgatggcatt gttggcttta
    35461 caataagtgc aactaatctt gactatgatt attttgatac aacagatgat tcttatgtga
    35521 caatagataa cactattgca gatcaagacc aagacaacat ccctgatgca aatgatactt
    35581 ttgttgggag tttaggtatt gttgaaacag aaggaattga tgatttaaat atcacaattg
    35641 atgacgacac aaatattgta ggaatattta ctggtgtaca aaccataaaa atttatgacg
    35701 cccaaacacc aattttggaa tttgatcata acttttcaga aagcacactt gatttatctg
    35761 taataaaaat tattaaaaca aatgcaggcg tggttgttga cttaaacggg caacttcaag
    35821 aagaccagaa caaaacactt tatcttgaaa ataataattt tatttcgctt tgtgtagaaa
    35881 atgaagcaat aacttcaatt tcagacatta gttctaattg cttggatgct aacgaatatg
    35941 attttactga atgcttaagc gaaagctatt ctagtaacgg aatagattgt gttctaaatg
    36001 gaaacctaat aacagtaagt aatcttagat attcaggaat acttggaaca gtaagaacaa
    36061 caagtggtgg aaattctatt tcaaaagagg cagatgatac agaagagatt atttctgaaa
    36121 taaacaccga cacagaaaat acagacaata atccaattat tgttacgcca gaagataaag
    36181 acccagaata taatgtggtc tcctctgata gcccagatta taaaccaaat gatgaaatgc
    36241 caccaataga ttactctaga aaaagcaaaa acacaacaac catttggata attacctatt
    36301 ttatttgtgc cttaataatt ggtataacaa tatatctatt cactaaaata agatacagat
    36361 aacactagaa aagaccatat ttaaaaagct tatgttcatc tatattacta taaattatat
    36421 aggtgattaa ctataattgt tgcttgctca ggaacagaaa aaatcgcaaa agaaattgca
    36481 aaaaaactta atctttcttt ttctaaaatc caaactacaa agttcccaga tggagaaatt
    36541 gatgttactt ttggaaaaga cttaaagggc aaagacgttt ttattgtaca gaattttcaa
    36601 aacactaaaa gtctaacatt aaatgacaag attgttgaaa ccttgtttgc aatatacact
    36661 gcaagagacc taaaagcaaa aagaatatat ctgattgccc catactttcc atactttaga
    36721 agcgacaaga ggtttagaga tggagaatgt gtttcaataa aagttatgaa taaacttttt
    36781 gagggctgcg ataagatctt ctgtgttgat cctcatcttc atagaatcaa aaatataaaa
    36841 aacgcattat ctaatggcat agaaattaat cttgacgagc caattattga atatcttaaa
    36901 aagaaaaaaa tacaaaaacc aatctttgtt ggaccagaca tcgagagttt acagtgggtt
    36961 gttaatgtgg ctaaagcatt taaagaaaag ccagtagttg ctttaaagga aaggaaaagc
    37021 gcaagggaag ttgtgatttc tttacctaag ataaagagcg caaaaggcag agacataatt
    37081 atcattgatg atattattag tactggccac accataattg aaaatattaa acaacttaag
    37141 aaatacgaac cacgaaaatt ctatgttatt ggaatacatg gtgtctttgt tgaaggcgca
    37201 ttatctaaac ttaagaaaca ttgttttgtg gcatcaacta acactcttga taattctgca
    37261 gaaaagattg atgtatctag ccagattgca aaaattgtaa ggaagaatat atgactgaga
    37321 aatatttttt agctagcgat cacgcaggct ttgagcttaa agaaaagatt aaagaatatc
    37381 taaagacttc aaaacttgac tttcaagacc ttggcccaaa gacatacaac ccagaagacg
    37441 actataacga ctatgcaaaa aaagttgtta agaagattaa agaaaataaa aatcattttg
    37501 ggatacttgt ttgtggaaca gggcaaggca tgtcaattca agcaaacaga tattctggaa
    37561 taagatgtgc actttgctgg aataaagaaa ttgcaaggca agcaaaagag catcttaaca
    37621 ctaatattat ttctatgcct ggaaaataca taaatataaa gactgcaaaa gaaataattg
    37681 agatttggag aaaaacaaaa accttgagtt tagaaaaaca caaaagaaga ataaacaaat
    37741 tggagatatt atgaaactac aattagcact tgatgtcaca gataagaaaa aagccttaga
    37801 gctttgtaaa aaaattagta aatatataga tattatagag cttggaacac cttttatcaa
    37861 gatctatgga cttaatggtt tagttaaaga ctttaannnn nnnnnnnnnn nnnnnnnnnn
    37921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37981 nnnnnnnnnn nnnnnntata agccagacta tattggcata cactctggaa ttgacatgca
    38041 gcacatggga attaaacctc ttaaaaccct tattgattta tcaaagatta tggatagtaa
    38101 aaaaatcgca attgctggcg gaattaatct tgaaactata gaaagcatag caaaatacaa
    38161 gccaggaatc attattgttg gtggagcaat aacaaatgct aaagatccag tttctgttgc
    38221 caagaaaatg aaagaaataa tgaaaaaata tgagtaaaga cattgacgat attcttgaaa
    38281 gaatagtaaa tgaaataaaa atttctttaa aggacattag tgaaattaaa actagcaggt
    38341 ttgtaaaaga aatcctaact tcaaagaaag tgtttgttac tggtcaaggc agaagtgggc
    38401 taatcgcaga aagctttgca atgaggctta tgcaaatggg aataaaaaca taccatatcg
    38461 gagacagcac aactcctgcc atctctaaaa acgatttgct tgttgcaatc tctggctctg
    38521 gtaaaacaga aatcacaaat atgattatta aagaagcaga taaagcaaaa gcaaaagtaa
    38581 tacttataac aaataagaaa acaacaaaag aaaaaaaaag aatcagaaac acagacttga
    38641 taattgagat taatgcgaaa gcaaagactg acaagaaaaa aaaatcaatt gaaccacttg
    38701 gaacattatt tgaacaagca agcctagtat accttgatgc agttattgtc ttacttatgg
    38761 aaagacagaa tgttaaagaa aaagatcttg gcaaaagaca tgcaaactta gaataaaacc
    38821 atataattat aagggttttg atagttaata taaaatatat tttagatttg gagcagaaat
    38881 gaaaacttac gttaaaaaag gaaaagtatc tatagatgaa accacaccag aagagatact
    38941 taatccggct acttttacga acactattaa aaaaaccgct cttcttaaaa aaataaatta
    39001 cgatttagaa aagagagaaa ctaaacctat agacataaaa aagacaattg ttcctgaaaa
    39061 aaaagaaaca atagacatta aagaaaatat aactagagaa ataaaaaaga taattataga
    39121 taatacagaa aaacagatta ttaaaaaaac ttatccaaat acagaactta aacacatacc
    39181 tctagataaa tcctattctg aaaagaaaga aatagaagac tataatctaa aaaatgaaaa
    39241 aattaataaa aaattaaaca ctggtctttc tgatagatta aaaccaaaat taaagaaaat
    39301 acatagaaac ccagaactta ctttttataa gtctattgac aatccaataa tttgtccaaa
    39361 accagaaaat ggctgggagt cttggcaaac ttttaatcct gcagcgatct tgattgatga
    39421 taaggttcat tttttgtaca gagctattgg agacgacggc atgtccagat ttggttatgc
    39481 aatgtctgag gacggttttg taattagaga aagactttct tatccagctt atgttcatga
    39541 aattgttaaa gggccttatg tttttttatc ctcgccatct ggcggaagct ggggtggctg
    39601 tgaggaccca cggcttgtta gagttgaaaa tgaggataga atctatgtta cttatactgc
    39661 ttgtgatggg ggcttaagag ttggcttaac ctcaataaaa gtttctgatt ttataaacaa
    39721 aagatggaac tggaaaaaac caaagataat ttctccacca ggcgagtgta ataaaaactg
    39781 gttaatcttt cctgaaaaga taaacggtaa atatgcaatc ttacatagca ttaattcagg
    39841 gaaaatacta attgattacc ttgataatct tgattttaaa gacggaaaat tcataaaaag
    39901 caaatatctt ctaacagaag tcagagctga ccgctgggat aaaaaagtta gagctgctgg
    39961 ccctccacca ataagaacca agtatggctg gttattgttc tatcatgctg aagataaaga
    40021 cgatcctgga aaatacaaac ttggagcaat gttactagat attaataatc caacaaagat
    40081 attatatcgc tgtaaacaac cagtacttga accaaaggaa tattatgaat gtgaaggata
    40141 taaaggtgga attgtttatg cacttggctc tgtgattaaa gacggaaagc taattattta
    40201 ttatggcagt gcagacaatt ttgtttgtgt tgcctatact gaatttgagc cattccttga
    40261 agccctaatc agaggagaga aaaagcctct taagcttaag attcttaaaa agaaataaat
    40321 ctgatttatt atgattatta aaagatatga taaaaatcca attctaaaac caaataaaaa
    40381 tagctgctgg gagtctcatt ctactttcaa tggctgtgca attaaaaaaa aatatgagac
    40441 atttttattt tatagagcaa tgtcatgtaa tgatttccat ccggttaatg agcagaagat
    40501 ggagatgtct tgtattggtg tttctagaag ctttgatgga ttaaatttta aaaatagaaa
    40561 agtgtttatt tatcctgaag aaagctttga taaatttggc tgtgaggatc caagaatcac
    40621 aaagcttcaa gacaagtatt atattttcta tactgctctt tcagaatatc catttagagc
    40681 aagcggaata aaagttgctg ttgctataac taaagatctt gaaaagattg aagaacggca
    40741 tcttgttacg ccatttaatg ctaaagcaat gactatgttt cctgaaaaaa taaataacaa
    40801 gatttggtgc atgtttacat atcacagtga ctctccacca gcacaggttt gctttgcaat
    40861 atttgataaa gaagaagata tgtggtcaag ggatttctgg cagaaatggt ataataatca
    40921 taacaagttt gttttaccac tacagagaag agaaaaagac caggtagaga ttggtgcacc
    40981 gcctattaag acaaaacacg gctggctatt attctattct tatatctatg attatttttc
    41041 ttcaaacaaa gtatttagta ttgaagcagt tcttctggac cttaaagacc caacaaagat
    41101 tattgcaaga accaaagggc cattactaac tacagaagaa tattatgaaa aatatggaat
    41161 ggttaatgat attgtttttc catcaggagc aacaataaat ggaaattaca taaatcttta
    41221 ttatggggca gcagacacca cttgctgtgt tgcttttgtt gataccaagt gtttgtttga
    41281 acagatgctt ccaaacactc taaataatat ttcatttatt agatcagaga aaaacccaat
    41341 aattaaacct aacaaaaaga ataaatggga agcgcgctca acttttaatc ctgcagcaat
    41401 atatcttgat gacaaagtgc atattgttta tagagcagta tcagataata atgtctcaac
    41461 aatgggctat gcaacttcta aggacggaat aactattgac tataggtcac cagaaccaat
    41521 ctataagcca agagcagaat ttgaaagctc tggttgtgag gacccaaggc ttacgcagat
    41581 tggagataac gtctatatgc tttatactgc ttatgatgtt agaacagcgc cacgtgttgc
    41641 tttaacaaag atctctgtta aggattttat tgctaacaac tgggacaact ggtcaaagcc
    41701 aatcttaata tctccaccag gaataagcga taaagatgct tgctttttcc cagaaaagat
    41761 taatggcaaa tattatgttt tacatagact tgaaaacgaa atagatcttt gcgcaattga
    41821 tgatcttgag tttaaaggca agaataaata tctagaagaa aacaactgga tggcaccaag
    41881 aaagggctgg tgggattata gaaaagttgg agctgcaact ccgcctataa aaacaaaaga
    41941 aggctggata ttattatatc acggcattgc tgaagaccac tcttatagtg ttggagctgc
    42001 tttacttgat cttaatgatc ctacaaagat acttgcaaga actgatgagc cgatctttaa
    42061 gccagaaaaa gattatgaaa aatccgggga tgttccaaat gttgtgtttc cttgcggggc
    42121 cattgttaaa gacaacaaaa tatatattta ctatggtggt ggagactctg ttactggtgt
    42181 tgcaagcatg gaaattgagt tgctactaaa aaaattaaaa ctttgcaggt gctaatatat
    42241 gaaagaaaca aaaaggcctt ggggtaaatt tctaaccttt gcaaaaaacc aaaaatgcac
    42301 agttaagatt cttgaggtta atcctggaga atccctaagc ctacaaaaac acaagcttag
    42361 agaagagcat tggtattttc tagaccctgg tattgttcag attggtaatt ctaagaaaaa
    42421 gattgctgca ggaaaagaag taataattaa gaaaaacaaa gaacacaggc ttgttgcagg
    42481 aaagaacaaa acaagagttt tagaaatttc ttttggtaat ttttcagaaa aagacgaagt
    42541 aaggttagaa gataaatatg caagaagata aggcatttaa aacaaatgac ataagaggaa
    42601 tataccccaa agaaataaac gaaagccttg cttatttaat tggaaaagca atagtctata
    42661 agtttaagcc aaaagttgtt gttattggaa gagacgcaag agcttcttct cctttgcttt
    42721 tttctagtgt tgttaaaggc gttcttgaaa gtggctgtga tgttattgat attggtctaa
    42781 ctaatactcc tttgttttat tattctgttg gagaactaaa agcagatttt ggaataatga
    42841 ttactgcttc gcataaccct ggacaatata atggctttaa gcttgttaaa aagggtgttg
    42901 ttccaacaac cgcaaaagaa atacaagata taaaaaatat aattcttgaa aaaaaagcaa
    42961 tatcttcaaa gcctaaaaag cttggtaaaa taatcagaaa agagatttct gaaaaatatc
    43021 ttaaaaagct attgtcttac tcaaaaatta aaaagaaact aaaagttgtt gttgacactg
    43081 gtaacggcat ggctgcacca attgtttcta aggccttttc taatttgcca atttctgtga
    43141 tttatattaa taaaaaaatg gacatgatta aacctaaaca cgaggcaaac ccattaaaga
    43201 tagaaacctt aaaggaatta caaaaagagg ttaagaaaaa caaagcagat attggtattg
    43261 cctttgacgg cgatggcgac agggttggct ttgttgacga taagggtgaa ataatcccta
    43321 tggattttat tactgcttta cttgctaaat atttcttaga gaaaaaacca aaatctaaga
    43381 taatttatga cattagaagc agcaacattg ttaaagaaac ggttcttgca agtgtcggaa
    43441 ttcctgtaga atacaaggtt ggccattctc ttattaaaaa aaagatgaga gagattaatg
    43501 ctgtgtttgc tggagagatt agtgggcact attatcttcc tgagttttat tattgtgaag
    43561 ctccaatctt tatttccata atcttaatga actatttaga gagagaaaag ctatcaaaaa
    43621 tgattttgcc atttagaaaa tactataaaa gcccagagat caattacaaa ataaagaatc
    43681 cagaaaagaa gataaaagaa ctaagaaata aatattcttc aggaaagata aaaacccttg
    43741 atggactaaa gatcgaatat cctgactggt ggtttaatgt cagaggtagc cacacagaac
    43801 cacttcttcg ccttaacctt gaagccaaca ctaaagagct tatggtaaag aagctaaaag
    43861 aaatcgaaga tataataaaa aaataagctc cagacgcgct cttaccc
//
LOCUS       DAOOON010000005        38686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_1202_length_38686_cov_13.027491, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000005 DAOOON010000000
VERSION     DAOOON010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 38686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38686
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_38686_cov_13.027491"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..576
                     /locus_tag="PLK55_01385"
     CDS             109..576
                     /locus_tag="PLK55_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35620.1"
                     /translation="MVLYKYCSRCYSLNISRDPNSNNQKCMQCGNIGIMPQDSIDKIN
                     VMKKSKDGQRYAAASQSARPLRTGSPGNDCEVIRKKDDPYNDDIDEIEVPKSKDNFED
                     DEEPEDNLESRQSNIMKRSSQQPTPERHGLSPKSNLSAKERLKEKGGKDWDLV"
     gene            602..904
                     /locus_tag="PLK55_01390"
     CDS             602..904
                     /locus_tag="PLK55_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35621.1"
                     /translation="MKTEKTKWYFLIAVALIVGAIVGYLATTKFSTTGDAKIGISDSC
                     VNEVDLLRDDASARCYCSVGTCTCGGDCPYAPNGTEKCNWTKKQYYKYVTDELSKK"
     gene            complement(907..1779)
                     /locus_tag="PLK55_01395"
     CDS             complement(907..1779)
                     /locus_tag="PLK55_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOZ35622.1"
                     /translation="MNKLYFGTAGIPGVSNAPIPKIKDQTTTGIMQVSSFSLDALEIE
                     FVRGVYLKLNNKEQLQEIRKTAEEKNTLLSIHCPYYINLSSLDPEIIRNSTGYITNSL
                     LVGQEIGSKIALFHPGFFQKQERAVVLEKVSFGLEKIISELDKRGNKEKINIGLELTG
                     KETQVGSLEDIFYFFEKYKAENVLPVIDFSHQHARFDGYFKDQKNINAFFEKLNSYPD
                     YVKQMHCHMSGIEYSPKGERNHVMLTDKANDFPYKKILEKLVEIKASGVVISESPIPH
                     QDALLLKKEYEKIK"
     gene            complement(1776..1967)
                     /locus_tag="PLK55_01400"
     CDS             complement(1776..1967)
                     /locus_tag="PLK55_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOZ35623.1"
                     /translation="MGFKIIHDKNKCVGCGACVAVCPDNWAYQGEKPVPKKTKVAEIG
                     CNQVAADSCPVSCIKIEEE"
     gene            2075..4297
                     /locus_tag="PLK55_01405"
     CDS             2075..4297
                     /locus_tag="PLK55_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35624.1"
                     /translation="MPDKYKKFKVSTENPGEIYSSISVDSRDFSKPSDKLTKRTSDKY
                     VTLCKKIYQKFPSLGVNGKYNEEYQKAIDFLDWDLTAEEYTATTKLVLFGGLFITLFI
                     SGLSYAFLYTILSEAFGNSMMALFLIFGLPAVIFIFVFLSFQKYPLKKVADEKIKALT
                     FVPEILGYMIMSMKLVPNLEKAIEFASEHGHGKIAEDLKKLLWEVKVGIKNSVAEGLD
                     DLAYRWGDISIELKKGLMKIRASVIESSESKRYQLLDQTMSETLESVKGKLEDYARTL
                     NQPATIMFYLGILLPLLLIIVLPVGSAFSGSALAQPIYLILIYNVIIPLACFFYAQGV
                     IKKRPPTYNPPNIPYDFPGLPKKNHLRVKNAQINVFIIIAILLVSCAVIGFTLQNAYG
                     VTREKILEREQGTTTPNWDCTREERQQQLLDPAFICADERNFWQDPKNSITPYYLIFF
                     ILFAVAASISLWAYATSVYKRKIQLATENQEKEFKDALYIIASRLGENKPIEDAFEHT
                     RRFLPKSTVAKDIFGKTVDNIRVLGLTLQSAVFDRNYGALKNNPSRIITSAMQLVVDS
                     VQMGVNVAAKTLISYSMQLRNTDEINRLLSILIAEITGTLNSMAKFIGPIILGVTTTL
                     HKVVISTLASVASSGAIQDMTQSLEGINTAELGMDLTSVSSFGTAINVEVIGKLASAT
                     SFTIIVAIYLIEIVLIMTYFTTMIEQENMTLVKYRISQTLPVAVILFIITVILANMVL
                     "
     gene            4294..4836
                     /locus_tag="PLK55_01410"
     CDS             4294..4836
                     /locus_tag="PLK55_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35625.1"
                     /translation="MNFKLSKKGDDSASEAYRLLIGFILGAAILVIIMNMINSTQNQS
                     ILISNQKLKEGLVSAVKSTGTSAKTPFVIEDLMLKGSISKTTITNYTGLPEECMAFVA
                     GPRLFSENDFNIIRIKPRYLKMDVWAYCDFMPPGTTQLPDGLDTNTQGQLTPDPDDCP
                     TYCVFFFNVKPDNGLYTTTS"
     gene            4904..5398
                     /locus_tag="PLK55_01415"
     CDS             4904..5398
                     /locus_tag="PLK55_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35626.1"
                     /translation="MNIFTKILFILLAIVILILGFIVGTFILTFIFVLLILMTLLFVI
                     IWLFNNLVSLFKSSIAKLIFKSVFSMVATYGLITILWLFTGKFFLQMDKTIALSVIWV
                     ITVLIFGVLWYLLHIRKHSKKHQYLWDDYKSTPDKLDSWDVLLMLVLPSILVYIFNFA
                     HAII"
     gene            5600..6385
                     /locus_tag="PLK55_01420"
     CDS             5600..6385
                     /locus_tag="PLK55_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35627.1"
                     /translation="MKNTKKEDQEYLDSMLEKHNISTKYDNEYYRKLDNTILQWCPFV
                     LILFIILSILWSSIVGSNKQIISFFIFTVIFIFAVLYIIFSFLFWIVELLSKKTKNYK
                     FNLIVKLSISIILAILITYTVSTPICAGCGGSTPTIATTQLLKSLIDNPGAESCTYPV
                     TFTISKSTLSAESITRDTGLEPEQVYFANPEDIVNFDAIDSQLLKYTATSSKKVAICA
                     ICSQDGKTGLEQALQENGISNTTINSIIEGETICAVYPRKSAN"
     gene            6484..7008
                     /locus_tag="PLK55_01425"
     CDS             6484..7008
                     /locus_tag="PLK55_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35628.1"
                     /translation="MLKLTKKGQASSTFQLLIAAVVALAILGVLLNILGVLPGLNKDP
                     TKTTKTLLTDQINNPGAESCTDPVTFTRSKSTLSAEGITVNTGIEMTQVYFDNPETIS
                     NFDPKDSILRYGASGSKKVMMCIVCSSDKSTLDQAIEANQKTDPADNFKTQPTELSSG
                     ETVCVIYPRKTSAS"
     gene            7034..7273
                     /locus_tag="PLK55_01430"
     CDS             7034..7273
                     /locus_tag="PLK55_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35629.1"
                     /translation="MNLKQELKRLWLDKRAQGDGASTLYMLIIIAIVALVLIAVIKPM
                     FNSSMKTSMNVADLPNQGTTATPTVNSTAPTTGSS"
     gene            7326..7928
                     /locus_tag="PLK55_01435"
     CDS             7326..7928
                     /locus_tag="PLK55_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35630.1"
                     /translation="MNKNKNIFLIKSSKAQASAPFEVLVAVILMGFVIMAGTWALINL
                     NQSVCVGNKRQSMSEMKGIIEDIIMGSDLAVKNVKFDIQPCFNEKFEAITLKTYTNET
                     LCTSYCNAGETCTLMEYVYDDDKTTKRPIAPMCLNLPTAINYASSAEGCEENSEEWLA
                     LNFGGSNPNSILTSGSYKIFMRSQTGTDSIRELCFLKKID"
     gene            7931..8587
                     /locus_tag="PLK55_01440"
     CDS             7931..8587
                     /locus_tag="PLK55_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35631.1"
                     /translation="MIEFSLSKLNLLIFVTAIAAIVIFFIATFNSSMKTRQSYELVYK
                     VGKEIKTGIENESYCTVKFITIPKTIQTNSGNSDTFNMRYKLNVSAYSVGDDSKRLVL
                     TVMDRKEKPKIYAAYDIDYNGSVALYASESCTSFPNCTITKSENFSVDFDPSKRDSID
                     TQILFAKIISNGNPIIFLIPCLEKKGIYTCREFINTKDLKTLIPCLNIVSDLVNYTDN
                     "
     gene            8596..9108
                     /locus_tag="PLK55_01445"
     CDS             8596..9108
                     /locus_tag="PLK55_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35632.1"
                     /translation="MNNKKGYIGAIGDDLPSLIPIFVGLTIFFAVFLNTYNVYRNNTD
                     LYSLRNDAINISGIIKENPIISDQNQFIKSCNRVKTKYNWNAFIIPLDLNSENYNGLT
                     IDDLEVENIMKHWAETESQPRKLICDSEGLGALRTALQTKDVTVYKYPITVQQQFYVE
                     PAWIYIVVWK"
     gene            9115..9918
                     /locus_tag="PLK55_01450"
     CDS             9115..9918
                     /locus_tag="PLK55_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35633.1"
                     /translation="MQLIKIKTRKGQAAITDALFFLIIIVALSVLMFRYSSTYGNRIE
                     QASSDLYYKEYTNSVLKTIFYVSAPLDFDLNLEKTKQNDYLMALVKQDYYAQGKLGFS
                     DMNDLKTDNYLDIAKYNLFHTIKATMRPLESQDYLFYIFNNEDSNFLYFTIKNTHFEI
                     SEANPNSGVDAVRGVTRYILTENPYHYYLCDPESYEDVRAVVSRSPKIFSSSIPLSFT
                     YNYSTYAQLGIKEDIVTSTFAMWPATVDINSDLLIPLNCKEVDPLSSNP"
     gene            complement(9940..10701)
                     /locus_tag="PLK55_01455"
     CDS             complement(9940..10701)
                     /locus_tag="PLK55_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35634.1"
                     /translation="MFIEKRGIIMDINKIKRRERRFYRKEENGEMVYGDTPITKPKKD
                     TKKMVPEKKETELKSTREIPEKTLKSIKENKKLNSEEKQKIIDKILIDLKNRIDPDID
                     EIAEMIYSQLSVRDVNVTQESVKQVVQSNAHYRGSRNITGSTSTNTVKTKENYDFLND
                     REELEKVIDDVVEENKKEDIREKAKKEKIVEKAIQNAKEKKSKETTKKSEDNIKDKIV
                     KNKESSKPKKEELKLDFGDDDAEDADSDDELGLKF"
     gene            10774..11310
                     /locus_tag="PLK55_01460"
     CDS             10774..11310
                     /locus_tag="PLK55_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35635.1"
                     /translation="MNELLLSTLNYIWVFIKLLLFSALLALPFIGLTYLFQKKFNWLR
                     LDKKLSFAKSLFIIIFVISYIALIIVYFVPVLDKFSTFTFFEGVLFVLIQIVRLFLVN
                     LLFTGMFFIIAMFISMLYDSFMEKKEKKKKTKKQKSEKLVFIDLWKSFAMVLFVVMLF
                     LIIIFPSLIAMILYLIFM"
     gene            11323..>14313
                     /locus_tag="PLK55_01465"
     CDS             11323..>14313
                     /locus_tag="PLK55_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35636.1"
                     /translation="MNESIKRVWIFFFLILFCNSVFAYGYNPDNSYYFAEKEISSDGV
                     VFDRGNGCMTKELSEKEVNALWGEILTKGFSADLLDSDQEANTNRENLDKNNLIVADA
                     DAKSGDVGAKMQVPSQQINPLEVPMLIGKTCYGSYDFGLNLNETLRVGRCDLDSNTSD
                     CYLSMPGQYRQNNLSASNVGGFFKEVKVVGKDLISGIKETVIKNEEDEIPGKENMGPF
                     SDLSSRELSKIEQTMLANDANTINTETWDAVSDEVLVNSSLTQNFAATMQTTCSGENC
                     YINTYSLFDKMFNQYFSADMVYSSVSPFLFNGAARIFNVPGVKNAVLKPINTLKDKGL
                     IKDGGFLDTLTKDPALFVKDPFGRISQSIKASKYANADVDRFVEFNLAHNEIMGRDIA
                     HYNMKGFTDNMFDSVQDGIGATSKFNKEMILDGTKNLSAAQRRVATDVAQEYADKLYS
                     SSALLKSKLANPITSDAINIYKKGIANGANINEILSNLTEEQYKALDDYVYTATKLSK
                     SLEIAMDGKFKWKDSLFDTATINNKVAKVYDNGVEREIKIENYKSLDGGSNALYKKEI
                     LQDGSVKIDTKVYGDEFKLFEYDRVNQVSQIKGAGKSVVTENMKVPLAGGEVNRQRLK
                     AVYKDLDKSGAIKINDAAGLTPFQKNTTAFVSFTDSVSGPQTISIHDYFTNSYTPLPG
                     TIELYPKGYNTILIDDVEIFDTVLGKKVKIKDAINPNTGKPYGPGYFDLDPLEVAAPV
                     LIPPSSGSTVVKKIDDTANRFSDVLKTMSHKEWVSGKGRDAINQLMRNYNGGSYQRFL
                     TQNLLVFGLNFAYWEVKSGGATIFGDTIGMTRWSMYQLPETYTALKIKHKENGEIYKD
                     AYLDFFANTGSDQGDLFMKYLNSMLFWSVNVTSKVVEGVGADWSTSVVDFLNARLQGQ
                     IRRSETDDIVLITNNISSGCNNQFCNFTIGANYIDQNTMNASLSAIELEELEKENKDA
                     KENTEDADAKENTEDADAKENTEDA"
     assembly_gap    14314..14413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14414..16663
                     /locus_tag="PLK55_01470"
     CDS             <14414..16663
                     /locus_tag="PLK55_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35637.1"
                     /translation="DAKENTEDADAKENTEDKGLLDFITKNKNTPKLKSQVDYKSISV
                     DTVTSDGIKTNNYLLENTSEKNLKVGQTLISFSHHTDYDGTMAGQSTEKAIDLIESRE
                     KEETCGQKVTNLTFLGAPIGSVIPKSFRNHRFVVGSMIAQKFAYLVLPGSGYFNSFIG
                     PAIVSDIPMQTMIMPEIHGCIDDEEGLYAHFFVSAQEEERQNKDSKNKVGEAIIDGTK
                     KVEEVVSKVTSGTELEKGVKAGSDKVKEYVEENIIEDPIVQATFTTGGGTNTAVKGQL
                     FFIEIGPKTKCMASGYNDKGVEVLKDKDTNIDVTIDKEKGILTVTDENGTRTVIGEGS
                     KDFVRLIGDNLGIPAKVIPRSLTYIPLPNNTEPLFEIDAHGNLTVKNSEFFDCLRAGY
                     EAQTGLTLNSTSKNLTPYLGIVKLTNDINPIGAYTSSFLGNSIISQGAGLPRLIANGN
                     TSKLTVLGNRSTTIGPIDGKENRTGKNISVQFEFGQLVYSEEAKAYIMWVETTTETSG
                     NDIASMKAEVTKTKAINGCENEEIGINFSTSPVKDNDQAAANTEKLNKALEKVGPFQM
                     FDTKTKTFIFYVSDPPECEQRLKIIDKETGEIYDAKISSFEQTPNGISVKTDDGQTHN
                     LEFSAEDGVPRLTYNGEKDTLTSAQGKNGSFWYDPETGNWYTTNGNLIPLNDAFRDGV
                     SFGVDGNGKVTGTPGTNVFNIGSAGSGKGSGGFNIPLTPESTVMIILYITVILSGFVL
                     IYMDRKKRK"
     gene            16716..18209
                     /gene="lysS"
                     /locus_tag="PLK55_01475"
     CDS             16716..18209
                     /gene="lysS"
                     /locus_tag="PLK55_01475"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOZ35638.1"
                     /translation="MGRLEEYQENRKNNFSSIVNMGIEPYPYTYDRTHVSKDIKEKYK
                     DLEPDKYTTEIASVAGRVLSLRSLGKISFIDLHDTYGKMQIVVKNETTSPENLELLKK
                     LDDGDIIGVKGKIFKTKRGELSIDAQDITFLSKAFMPLPEKFHGIQDAELKYRQRYLD
                     LMTSKESRDIFFIRAKIIKIIREILDKKGFLEVETPLLQSLYGGANARPFITESFVWK
                     RQLYLSISPELYLKRLLVGGFDKVYTICKNFRNEGIDRSHNPEFTMLECYEAFKDYNS
                     AMDLIEEIYETIALELNGSTRVTYLEQTFDFKRPWKRLTMAGALKEYANLDVDKLSDS
                     DLANLLKENRLELETYKRGLAISELFEHFCENHLLGPVHIIDHPKETTPLCKLHRSNK
                     DLIERDEPYVNYSEAGNIYSELNDPMLQEKLMLDQADQGRGKGENHPVDTDFIESLKY
                     GMPPAYGLGLGIDRLVMIFTNSANIREVILFPMMKEEGDGLEDNEKK"
     gene            18280..18501
                     /locus_tag="PLK55_01480"
     CDS             18280..18501
                     /locus_tag="PLK55_01480"
                     /inference="COORDINATES: protein motif:HMM:NF021415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HOZ35639.1"
                     /translation="MSQKCIICGRAIGNYGIYCPKCQEDLEKKIQKREKFEEVYRSAD
                     FFERMKLKETKDALKARQNRLAQKPVSKK"
     gene            complement(18633..19253)
                     /locus_tag="PLK55_01485"
     CDS             complement(18633..19253)
                     /locus_tag="PLK55_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35640.1"
                     /translation="MKKKIQTKISKYEEKKKTNFFSKNNRVKIIIWVLAILCVLLIIT
                     FSLKYLNNNNAETNTQETMPELGINEETENPNLETTPVIISGEITKSSKLEDFYATSK
                     PSFIVFAGTYCGHCQKLLPELEKEIWNNYSNKANIWVEIIGDGEFPVAMPQGVNSNII
                     YEEIMGDCRYVPAYVVLDKEGNQILRSCGSEKTIAEIKAALDSQLN"
     gene            complement(19255..20475)
                     /locus_tag="PLK55_01490"
     CDS             complement(19255..20475)
                     /locus_tag="PLK55_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOZ35641.1"
                     /translation="MFSNRPLFDYITKKEFTKIKKFAKGKKTPFLIIDLQKIEEKYDE
                     LIKNMPFAKIYYAVKANPEDDIIKLLARKGSNFDVATNYELDQLLRLKISPKKISFGN
                     TIKKEEDIAYAYKKGIRLFATDSISDIKKLAHAAPGSKVFFRIIMEGGSADWPLSRKF
                     GAHPDMIYHLIFEAKKLGLHPYGVSFHVGSQQRDIGQWDQAIAQCKYLFDAVKQEGIE
                     LKLINMGGGFPANYISPTHDLETYSKEITRFLEEDFGEHFSKKLEIIIEPGRSIAADA
                     GVIVTEVVMISKKSLSNQFDWVYLDVGKFGGLIETLDESIKYPIFTENYRKPPKEKDC
                     REVILAGPTCDSTDTLYEKHKYLLPKSLKEGDRLYILSTGAYTRSYSSIYFNGFPPLE
                     IYIMKKGSKKKKEE"
     gene            complement(20562..20804)
                     /locus_tag="PLK55_01495"
     CDS             complement(20562..20804)
                     /locus_tag="PLK55_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35642.1"
                     /translation="MVKKKRQIKKVKTEKNIKYEIYWDYFSLVMFLVFSTLFISMLII
                     EIFFVLGKSALWLTIPVTAILWALSYLLARILFKKK"
     gene            complement(20818..21495)
                     /locus_tag="PLK55_01500"
     CDS             complement(20818..21495)
                     /locus_tag="PLK55_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOZ35643.1"
                     /translation="MADKNGNGVTGDIPIATVRSELLAIIRKKNQKATEDGFITREEL
                     KIARQEYIESILKKEKGELNKIDKQVVKSLVEHESIVKDIMQEKENLTVGQKLADGVA
                     KFGGSWGFIISFGVFLIIWIGINSYFLLQKPYDPYPFILLNLILSCLAALQAPIIMMS
                     QNRKDQKDRIRAENDYKINLKSELEIRHLHEKMDNLVQKQWQRLLEIQRLQLDIMEET
                     QKERKKR"
     gene            complement(21507..21749)
                     /locus_tag="PLK55_01505"
     CDS             complement(21507..21749)
                     /locus_tag="PLK55_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35644.1"
                     /translation="MTLNLAIIGLAIIFIAWLFQFFSMNKRREISTVFIIAYIIGTIL
                     LVIDGYKVGLIANTIINSIIAIAAICVLIRFSKPKK"
     gene            complement(21746..21970)
                     /locus_tag="PLK55_01510"
     CDS             complement(21746..21970)
                     /locus_tag="PLK55_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35645.1"
                     /translation="MFPGYWSEGAGDKTCSKCLRFRPNVTNKNKGTCVGRDVVATGGC
                     EKFAKKKTTKVATIKKKTTNIIKTKKKAKK"
     gene            complement(22054..22389)
                     /locus_tag="PLK55_01515"
     CDS             complement(22054..22389)
                     /locus_tag="PLK55_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HOZ35646.1"
                     /translation="MKKILKMTKKITKIKLTEEELKRMKNKLKRQQTQFKKEQEQMRK
                     EVNEKLITLITGAFSLVAALAWNETIKTIFQRVFGTAQTVWAMIIYAVLVTIIAVWIT
                     YWMTKRNKN"
     gene            22456..23199
                     /locus_tag="PLK55_01520"
     CDS             22456..23199
                     /locus_tag="PLK55_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35647.1"
                     /translation="MKTPIMPTINYKKFSWNELDKIDQNTITQISEIDNLFYRSSSDL
                     EEYLRKIKINADKTIIYLCYRGKELVGAVFGTLNRVSEFCNKDYKIKAKPKDYMFLLT
                     EFFVKPGFQNNGIGTALMARVFADQRAKYDSKFIYMKAFRKTQTINKKLTGITPFIVP
                     IKEVAEIKRIVNNLSIIEKRKKRNISHNKSMKTGALKRKIFRNFKNSKYKYDLIETSN
                     PSITDVKDHYIVINKNPKYKRKQIRSIAK"
     gene            complement(23207..23764)
                     /locus_tag="PLK55_01525"
     CDS             complement(23207..23764)
                     /locus_tag="PLK55_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35648.1"
                     /translation="MNKKVKSKKLENVVYNIIMFTLIFALVISFVFTIDKLFLERPKD
                     NCYLQDYNYKPEVITNIDANCSYNQEYINSCYKSEGNIVYKKDCQMECSYCYRDYNKI
                     LETYNNRINIGRMIFTFLLALLMAFILIKDKIIRYSILSASLVSLFVATISATKLLNK
                     MLPVVIIAEFLLVIFIYRKTSKEKK"
     gene            complement(23764..25113)
                     /locus_tag="PLK55_01530"
     CDS             complement(23764..25113)
                     /locus_tag="PLK55_01530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ35649.1"
                     /translation="MTNTNNNFEGLVKDKQILQAIHNIGIKTPTEIQEKVIPAILEGK
                     NIIAQSATGTGKTIAFLAGVLPSITKQKKVQLLIIVPTRELANQVYEETKKIAKVKEL
                     QACAIFGGASVGNQARQLKYADIVIGTPGRIIDQFERRNLKVNNIKYLILDEADRMCD
                     MGFSEDISRIIEQLPKERQTLLFSATITNDVSNIERKYISHAQRIVVQTYVDPKNLLQ
                     DYYLVKQNQKFSLLLHLVKQHSKKAIVFCNTRSEVDVIYNNLKEQRVECFKLHGGLEQ
                     NKRSGTIEKYHANADGLLISTDVSARGIHIDNLEYIYNYDLPKDNTQYIHRIGRTARA
                     GKKGRAISIITQKEEEDFLRMCKKFGFIVEAKVLPSIVPITLKRTKEEERALERINRY
                     QERREHFSKDRKDRKKENQKKMVIGELTEEDIKKKRMDFFKHKKKIRQKKYTNRGRR"
     gene            complement(25130..25420)
                     /locus_tag="PLK55_01535"
     CDS             complement(25130..25420)
                     /locus_tag="PLK55_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35650.1"
                     /translation="MRIIKKTTRKIKNKIKEKYKLKPNEKAILKNLEKIVDQRLKEYM
                     RKYSLYLSSNPRINPQPQLNMEAATKELNEAITRYNRYRKEYGLDPVNYKKK"
     gene            complement(25500..25700)
                     /locus_tag="PLK55_01540"
     CDS             complement(25500..25700)
                     /locus_tag="PLK55_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOZ35651.1"
                     /translation="MAGKRDTISLIALILIIVGGLNWLLVGLFSFNLVTTVFGAIPIL
                     VTIVYTLVGLAAIWAIIKFKSI"
     gene            complement(25787..26452)
                     /locus_tag="PLK55_01545"
     CDS             complement(25787..26452)
                     /locus_tag="PLK55_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35652.1"
                     /translation="MPPVSISFRRHAQKTKNGKNITPKGKEDIVKLARESKPAKIYSS
                     TENRTIETAEILEKLTNTEYKLRINKELRGHKYFSGERIKTTKRDNLLTFFKRVSEKL
                     GVKDSIVIKIWLNGNFPKRIVPKPEVIADQIIKDRFKLPNRVNKMTNRTIIFENITHD
                     IVIAALFQRLSGIKYTKAFKHDFPKELEALTIEISKEGKAIMQFRDYKKEITDRLNAI
                     LTN"
     gene            complement(26493..27104)
                     /locus_tag="PLK55_01550"
     CDS             complement(26493..27104)
                     /locus_tag="PLK55_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35653.1"
                     /translation="MPVTRPRPVVKPKIVRKGIIKRNISIGPNATLGGKPVFSTEKGA
                     KNYRAFKRAQILKQKGEYDFKLKRGLIKSNQSMGPDIFKSDGKPFGYTTETKANNNRT
                     LRRKLSGQDIKSYNATLNGEGVWSYKGAIRYPPKYSRTKVLEIRFLVSEALKKPKITT
                     KILTARGINPKTLRFAGFSYKEIQSIYGEQIADRVFNFNKSKN"
     gene            complement(27270..27497)
                     /locus_tag="PLK55_01555"
     CDS             complement(27270..27497)
                     /locus_tag="PLK55_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOZ35654.1"
                     /translation="MSELPIVSSLKLTKVIIKLGFKEIRQKGSHKIFSHSDGRLVVIP
                     MHSNKPVPEGLLNKIIKQDLRLSKDEFLDLL"
     gene            complement(27494..27718)
                     /locus_tag="PLK55_01560"
     CDS             complement(27494..27718)
                     /locus_tag="PLK55_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007306954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOZ35655.1"
                     /translation="MLNNSFDVLIEEDKETKQYYGSVPNLPGCYSTGDTVEELITNMK
                     EAISLYLEVKKEEKSKILNNNVLGMIRVTL"
     gene            complement(27969..29141)
                     /locus_tag="PLK55_01565"
     CDS             complement(27969..29141)
                     /locus_tag="PLK55_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35656.1"
                     /translation="MDAKEEIYPFIEKTIKEKTYCTKNDLSDLIISKFNSTNDISEER
                     ARDIIERWFKATDKDTFDKLKATKFKKAFDLDILVNGNDMIYYYKRAFDENQILYAKN
                     LIKKNRFDLAQNIILHRTLNLNEFYDIFDLLNQKYPIDFIKLVLDTLSDNIKEQDSKS
                     IFPAITEGKKEKLITKIDLFYTTNYAPLSIYLEECPDIKEYNKIDEQVNKLRGECSNT
                     FYYDTSKNTPSQNKLLKLEEERAKLSTKIYEYNKEIELKKSIRGKLIEIIALLKDKHK
                     LYDYFTSDIDQYKIYINKASRSNEELEDMCKLKTVHPPIALEFTSGDKDVDRLYRKKS
                     ELYYQSNQLYTLICACYSKGVVNLWFSNSSEFNKGKLICDYEKYADIQKIINVVCR"
     gene            29350..29592
                     /locus_tag="PLK55_01570"
     CDS             29350..29592
                     /locus_tag="PLK55_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35657.1"
                     /translation="MSERKSIEGTILSISNKVNPKGVPYSIYKISTLEGKELDLFQWN
                     NNFRLLNKLCRFEIESNTKDNKEYNSIIEIKEVKKE"
     gene            29589..29864
                     /locus_tag="PLK55_01575"
     CDS             29589..29864
                     /locus_tag="PLK55_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35658.1"
                     /translation="MKVYELKDTTPYPKLKHDNTVFELKTQSDFEQAILDNDGEIFIE
                     DTKCYLLTKNNRCYVLFYKDKGPIVRMLKDRMDGGNSNDNNRRNISK"
     gene            29833..31395
                     /locus_tag="PLK55_01580"
     CDS             29833..31395
                     /locus_tag="PLK55_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35659.1"
                     /translation="MTTIEEILASNPTQYSNLSMGIKDGLFYYGKMVEHKGQLLEMMV
                     LSNRQLLTNYSSLNTKTDKRPNEINNFGIFYQDLPINIANNDWSNDSITKFISSNNKL
                     MNKKEVYNKVKEQITKYMDLYDERISSMVACWCMGTYIYNLFSGYGYILLTSQKDSGK
                     SKLLKIIGQLSRNSIYLSSPSESVLFRMINGNRPTLLLDDFETLKNEERTHYILQILK
                     IGIDEDGAIPRNEKYKDTFVPKNYNCFCPKALTNTCSLDPITISRCITINLLPTNTEK
                     GKLKPNKRDLIWQEIRDDLHILIMENYQEIIDIYNNESFGDFNSRQLDTTKGLLSIAK
                     FIDDSVYQEVSEFLKESFKDRDLQDLSSDWEYLLFKQLYEKVPSPRWCSTKEMNEWLK
                     EVAIPTDYNKGFNQYIGFVLSKLSSLFPKQRHNDGIMYLISPKSVQEYMSLKKYPCTL
                     EGSQDTQPTLDFKDEPFRELKNEIMDTCVNCNQRTYVKYTSKVVKGRNICEDCVKSEA
                     KRKGKELLDSMY"
     gene            31425..31859
                     /locus_tag="PLK55_01585"
     CDS             31425..31859
                     /locus_tag="PLK55_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35660.1"
                     /translation="MASTKEGKEISKMNAVKNGLYLKFTDFFPCNLCINREKCSDFIP
                     GGSCSIDSELFNQLVTTEIDELKVLDQLINYNMVRLSRATEQSRNRPDHIELSRISAE
                     IRNLIQARFIIKRRIEGEKEYARTKVLQPMSDQSQLHLFQEK"
     gene            31879..32082
                     /locus_tag="PLK55_01590"
     CDS             31879..32082
                     /locus_tag="PLK55_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35661.1"
                     /translation="MISEDIYGFKKTSIDKWYSLVNLDRTEDKNKAKFYKMVNKEVKE
                     LIQDIQEQIAEEKENEDEKNARL"
     gene            32060..32419
                     /locus_tag="PLK55_01595"
     CDS             32060..32419
                     /locus_tag="PLK55_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOZ35662.1"
                     /translation="MKKMQDFKKLKIWQLGREFTKDIYDITEHFPKHEFHSMTNQLRR
                     ASYSIPSNIAEGCGKSSQKEFGRYLSIVSGSIKECECFLILSKDLEYITNKEFNELNG
                     KLEIIGKMCNKLLIKIN"
     gene            complement(32416..32763)
                     /locus_tag="PLK55_01600"
     CDS             complement(32416..32763)
                     /locus_tag="PLK55_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35663.1"
                     /translation="MNKYDFSDLAKHNEQIAKDSESNCKAWSSIILFYSAVHWVNFFV
                     LNKFPNLQKPGTHPEREEFICKNANQIYKAYRHLRTISESYRYCPPDWKNADKEFGKI
                     KSDYTYIKQYVNS"
     gene            complement(32772..33143)
                     /locus_tag="PLK55_01605"
     CDS             complement(32772..33143)
                     /locus_tag="PLK55_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35664.1"
                     /translation="MYINKQANTNTLGLIDECYIGINKQLYNPITIITKVYAIKFYFH
                     LVSSLPFVKKLYVMNDRNVIRFWTLIDPNDYDSRDKIYDLELELRERFKDEKIKFEFN
                     VFSDNSYFIHQISENAKALIK"
     gene            complement(33151..33516)
                     /locus_tag="PLK55_01610"
     CDS             complement(33151..33516)
                     /locus_tag="PLK55_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35665.1"
                     /translation="MDKEILIDFITAYSKLLKIISSFINEVYILESNDSEKLKKMSEV
                     LNDEELLISLIKNDSPELSEIYKDLIFNLKMATIDKNLLNLSLSEKKDYADKLKLASE
                     NLVKISEQIKTLDLKAQTK"
     gene            complement(33678..36146)
                     /locus_tag="PLK55_01615"
     CDS             complement(33678..36146)
                     /locus_tag="PLK55_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOZ35666.1"
                     /translation="MAEYIEDYISGIKVKATPEEIDAVQTFSKQLVEDYGYPKVNIQT
                     RPQFRVKIRPSDTKKEYPIDIAIFPNSKKQEDEIYIIVECKKKNRKDGKTQLQDYLRF
                     SKAYLGVWFNGDERLFLRKIEKSGKIEFEEIPNIPRYGQRIEDIGRFKRKDLKPTHNL
                     KATFKAIRNHLFANTIGATRDEVLAQQLINLIFCKIYDERFTEPNEIVTFRAGVDEDT
                     ETVKKRILDLFNKVKRKYKEVLDDNDTITLDSNSVAYVVGELQNYCLIEAERDIIADA
                     FETFIGHALKGGQGQFFTPRNVVKMMVDILDPNDEDLIIDPACGSGGFLIESLRHVWK
                     KLDAEGEKYHWNKNNLQEEKMEVALNRIRGIDKDYFLSKVAKAYMAIIGDGKSGIFCE
                     DSLENPNHWNTTTKTKVHLGDFSILLTNPPFGSKIPVRGEDKLKQYALGHKWKLNNKW
                     EIGKLKDKEAPQILFVERCLQLLKDGGQMAIVLPDGIFGNESLGYVRQYICEHTKINA
                     IIDVPLETFMPNTSTKTTILILEKQPLNKIPKDYPIFMSVIENCGHDRRGKENEIDDI
                     KNVSEEYKLWKKEPKKYKSKDSYFNITFSQIKDYDLWIPKYLFPRYIYPIQNLKSKGI
                     PTITLGELFDFKKGIEIGSDNYIEYIFKKETDIPFIRTSDLVNYTINRTADLYLSKDL
                     FNQINFEIKEKDILFTKDGKIGMFAIASTNTNFLISSGILKCRLKKSCISYIKEKYKL
                     TITPEYIFSVLITKEIGLYQSLRRTVIASTIPHLNLERLKDIEIPLFDQRIIDENTKK
                     INEILELDRRKIDLVESANKLFRY"
     gene            36273..36548
                     /locus_tag="PLK55_01620"
     CDS             36273..36548
                     /locus_tag="PLK55_01620"
                     /inference="COORDINATES: protein motif:HMM:NF026297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOZ35667.1"
                     /translation="MKKDIAELVSFIVRGSNRIIILNCLALEELTPSQIKKKTNLYLS
                     HISRVIQELLDRKLIICINPDSPMVKFYAITKKGKTIIELVRKYKKE"
     gene            complement(36553..37560)
                     /locus_tag="PLK55_01625"
     CDS             complement(36553..37560)
                     /locus_tag="PLK55_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35668.1"
                     /translation="MVNVNNLIVKGIFGYPCKDTSDQKFAELYNGIQLELKNYMEKVG
                     YLTDDITMVDVLEHSYSKIHSNPKLFSTVIHYSMKRVYTPDLKYKLKSLLKVKTISDE
                     QKLYYNWLKGIRAELPYNIDLLFKWDSKESRIIIEVISMPVIYFQITQLNRKSDISNF
                     EYSKILFENIKFLNELSTIISTTVISEPKSLEYYINVPISNKLSEYGYEKVSELLIEG
                     YRYLEMGDKEKTIHKLIGVIEHFFEEFSKKTTDESCGLHKPEDNMRKIQKKMIISQEI
                     ESTIEYLVYTRIYKKLKDKDHKQEEDIDYNVLKLYFSYVESTIEYLLEIQKLKMHTTS
                     K"
     gene            complement(37625..38614)
                     /locus_tag="PLK55_01630"
     CDS             complement(37625..38614)
                     /locus_tag="PLK55_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOZ35669.1"
                     /translation="MLEVCNVSGPDDIYRTEHRLSRAIFTLQNNLEVVEENRNAILDY
                     VISMKAQGIKSIRLSKSIYMLRDMSNVIQIPFAIATKQDIEYLMFTIEQRDYAFWTKS
                     DYKIILKRFYKWLLGDDETAPKIVSWIKVKDSKSNILPEELLTEEEVLKLVATAKFTR
                     DKAFIYLLYESGARIGEMLTLKIKHITFGEKISSILVNGKTGQRRIPIIKSVPYLKEW
                     IKEHPFGEDSESNVWCKMIRRNQQGRGPKEIPSYSVVRKTLNETFKKSNIEKKSNPHL
                     FRHSRATYLANHLTEAQLKQFFGWTQASDMASRYVHLSGRDLDSAILNLGGIV"
BASE COUNT        13261 a         5575 c         5688 g        14062 t
ORIGIN      
        1 ttttcactcc gttttgttat gctatattat agattaaata aaatatcctt ttaaatattt
       61 gtatttatca tattaattgt aaataaattt atttaagcag gtgaaaaaat ggtattatac
      121 aaatattgtt caagatgtta ttctttaaat atttcaagag acccaaattc taataatcag
      181 aaatgtatgc aatgcggtaa tattgggatt atgcctcaag atagtattga taagattaat
      241 gttatgaaaa aatcaaaaga tgggcaaaga tatgcagcag catctcaaag tgcaagacct
      301 ttaagaactg gtagtccagg taatgattgt gaggttatta gaaagaagga cgacccttat
      361 aatgacgata ttgacgaaat cgaagttcca aaatctaaag ataattttga agacgatgaa
      421 gaaccagaag acaatttaga atctaggcaa tcaaatatta tgaaaagatc atctcagcag
      481 ccaacgcctg agagacacgg gctttctcca aaaagcaatc tttcagcaaa agaaagatta
      541 aaggaaaagg gcgggaaaga ctgggattta gtttaaattt aatcaatttg gaggaaataa
      601 aatgaaaacc gaaaaaacaa aatggtattt tttaattgca gtagctttaa ttgttggtgc
      661 aattgttggt tatcttgcaa caactaaatt ttcaacaaca ggagatgcaa aaataggtat
      721 ctctgatagt tgtgtaaatg aagttgatct tttaagagat gatgcatccg cgagatgtta
      781 ttgttcggtg ggaacttgta cctgtggagg tgattgtcct tatgcgccaa atggcacaga
      841 aaagtgtaac tggacaaaaa aacaatatta caaatatgta acagatgaat taagtaaaaa
      901 ataaaattat tttatttttt catattcttt ttttagaagt agggcatcct ggtgtggtat
      961 tggactttct gagattacaa caccgcttgc ttttatttct actaattttt ctagtatctt
     1021 tttgtaaggg aagtcgtttg ctttgtctgt taacataaca tggtttctct cgccttttgg
     1081 agagtattca attcctgaca tgtgacaatg catttgctta acataatcag gatagctatt
     1141 gagcttttca aagaaggcat taatgttttt ctgatcttta aaataaccat caaaacgagc
     1201 gtgctggtgt gagaagtcaa ttactggtaa aacgttttct gctttatatt tttcaaaaaa
     1261 ataaaagata tcttcaagag aaccaacttg tgtttctttt cctgtcaatt ctaaaccaat
     1321 atttatcttc tctttgtttc cgcgcttatc aagttcagaa ataatcttct caagaccaaa
     1381 agaaactttt tcaagaacaa cagccctctc ttgtttttga aaaaaaccgg ggtggaacaa
     1441 tgcaattttt gagccaatct cttgtccaac taatagtgag tttgtaatat agcctgtgct
     1501 gtttctaata atctctggat caagagaact aagattaata tagtatgggc aatgaatact
     1561 aagaagagtg tttttttctt ctgctgtctt tcttatctct tgtaattgtt ctttattatt
     1621 aagcttaaga taaacccctc taacaaactc aatctctaaa gcatcaagag aaaaagaaga
     1681 gacttgcata attccagtag ttgtctgatc ttttattttt ggaatggggg catttgacac
     1741 accaggaatt ccggcagttc caaaatataa cttattcatt cttcttcaat ctttatacaa
     1801 gaaaccgggc aactgtctgc tgcaacttga ttacaaccaa tctctgcaac ttttgttttc
     1861 tttggtactg gtttttctcc ttggtatgcc cagttatctg gacaaacagc aacacaggct
     1921 ccacaaccaa cgcatttgtt tttatcatgt attattttaa atcccattta atcgctcctt
     1981 aagttttatt atattaataa gatataaaat aaacatgtat ttaaatgttt ctttccttta
     2041 taaatatatt ttaatattaa aaaacagatg tataatgcct gataagtata aaaaattcaa
     2101 ggtctcaaca gagaatcctg gcgaaattta tagttctata tcagtggatt ctagagattt
     2161 ttctaaacct tcagacaagc ttacaaaaag aacatctgat aaatatgtta ctttatgtaa
     2221 aaaaatatac caaaaatttc caagccttgg ggtaaatggg aaatataacg aagaatacca
     2281 aaaagcaatt gattttcttg actgggactt aactgcagaa gaatatactg caaccactaa
     2341 acttgttttg tttggagggc tatttattac attgtttatt tcaggattat cttatgcttt
     2401 tctatatact atcctgtcag aagcctttgg taattctatg atggctttat ttttgatatt
     2461 tggactacca gcagttattt ttatttttgt ttttctttca tttcagaaat atccattaaa
     2521 gaaagttgct gatgagaaga taaaagcact aacttttgtt cctgaaattt taggttatat
     2581 gataatgtct atgaagttag tgcctaatct agaaaaagca atagaatttg cttcagaaca
     2641 tggacatgga aagattgctg aggatcttaa aaaattactt tgggaagtaa aggtcggaat
     2701 aaaaaacagt gttgccgaag gattagatga tttagcttat cgctggggag atatttctat
     2761 agaattaaag aaaggattaa tgaagattag agcatcagtt attgaatctt cagaaagcaa
     2821 gagatatcaa cttcttgatc agacaatgtc tgaaactcta gagtctgtaa aaggcaaatt
     2881 agaagattat gcaagaactt taaatcagcc agcaacaatt atgttttatc ttggaatatt
     2941 attgccttta ttattgatta ttgttttacc tgttggctct gcattttctg gttctgctct
     3001 tgctcaacca atttatttaa ttttaattta taatgtaatt ataccattag cttgtttttt
     3061 ctatgctcaa ggggttatta aaaaaaggcc accaacctac aatccaccaa acatacctta
     3121 tgattttcca gggctgccaa aaaagaatca tttaagagtt aagaatgctc aaattaatgt
     3181 ttttataata attgcaatac ttcttgtaag ttgtgcagtt attggtttta cgctacaaaa
     3241 cgcctatggt gtgacaagag aaaagatttt agaaagagaa cagggcacga ctacaccaaa
     3301 ttgggattgt acaagagaag aaagacaaca acagcttcta gatcctgcct ttatatgtgc
     3361 tgatgaaaga aatttttggc aagatcctaa aaattctata actccttatt atctaatatt
     3421 ttttatattg tttgcagttg ctgcttccat ttctttgtgg gcatatgcca caagtgtcta
     3481 taaaagaaaa atacaactag caactgaaaa tcaagaaaaa gaatttaaag atgctcttta
     3541 tattattgca agtagacttg gagaaaacaa gccaatagaa gatgcttttg agcataccag
     3601 aagattttta cctaagtcta cagttgcaaa ggatattttt ggtaaaactg tagataatat
     3661 tagagtctta ggtctaactc ttcagtcagc agtttttgat agaaattatg gtgctttaaa
     3721 gaacaatcca tcaagaataa taacaagtgc catgcaattg gttgttgatt ctgtgcagat
     3781 gggagttaat gttgctgcaa aaactttaat ttcatattct atgcagctaa gaaatacaga
     3841 cgagatcaat cgtttgcttt ctattctgat tgccgaaatt acaggaacac taaatagtat
     3901 ggctaagttt attggcccaa ttatccttgg tgtaacaaca actcttcata aggtggttat
     3961 ttctactcta gcatcagttg caagttctgg ggcaatacag gacatgactc aatctctaga
     4021 aggaataaat actgcagaac ttggaatgga tttaacgtct gtatcttctt ttggtacagc
     4081 aatcaatgtt gaggttattg gtaaacttgc aagtgcaact tcttttacaa ttattgttgc
     4141 gatttatttg attgagattg ttctaataat gacttatttt acaacaatga ttgagcaaga
     4201 aaatatgaca ttagtaaaat ataggatttc acaaactctt cctgttgctg taattttgtt
     4261 tataattaca gtgatattag caaacatggt attatgaatt ttaaattaag taaaaaagga
     4321 gatgattcag cttctgaagc ctataggcta cttattggat ttattcttgg tgcagctatt
     4381 cttgttatta ttatgaatat gattaacagt acacaaaacc aatcaatatt aataagtaat
     4441 cagaaactaa aagaagggct agtctctgca gtaaagagta caggaacaag tgcaaaaact
     4501 ccttttgtta ttgaggattt aatgcttaaa ggaagtatct ctaaaactac aattacaaat
     4561 tatacaggtc ttccagaaga atgtatggct tttgttgctg gtccaaggct tttttccgaa
     4621 aatgacttta atattataag aataaaaccg agatatttga aaatggatgt ttgggcatat
     4681 tgtgatttta tgcccccagg aacgacacag ctaccagatg gattagatac aaacacgcag
     4741 gggcagctta caccagatcc tgacgattgt ccgacatact gtgtattttt ctttaatgtt
     4801 aaaccagata atgggcttta tactacaacc tcttaaaatt aatatttata aacaatacca
     4861 cactataata ttttaattaa atacagaaaa tgtgaaaaac catatgaaca tctttactaa
     4921 aattttattt attcttttag caattgtaat acttatactt ggctttattg ttggaacctt
     4981 tatcttaact tttatatttg tattattaat cttaatgact ttattgtttg tgattatttg
     5041 gttatttaat aatctcgtaa gcctatttaa aagtagcata gctaaactta tctttaagtc
     5101 tgtattctct atggtcgcta cttatggttt aattacaata ttgtggttat ttactggtaa
     5161 gttcttttta cagatggata aaacaatagc acttagtgtt atttgggtta ttacggtttt
     5221 aatctttggg gtattgtggt atttattaca cataagaaag cattcaaaga agcaccaata
     5281 tctttgggat gactataaga gcactccaga taaactagat agttgggatg ttcttttaat
     5341 gcttgtattg ccatcaatat tagtttatat atttaatttt gctcatgcga taatttgagt
     5401 ttaggttaaa aaacaaccta aaataaagat ttacaatcaa gctagttttt ggtttattat
     5461 taactttaat tttattatta atataattat aattcaataa accttgcaat atcaatagct
     5521 ttttaaaagc ttatatcttc tatttctttt atttaatctt agtactaaat actattttaa
     5581 ataaaattag tgaaaatata tgaaaaacac aaaaaaagaa gatcaagaat accttgattc
     5641 aatgttagaa aagcacaaca tatctacaaa atacgataat gaatattata gaaagttaga
     5701 taatacaatt ttacaatggt gtccatttgt attaatatta tttattattc tttctatttt
     5761 atggtcttct atagtcggca gtaataaaca aataattagt ttttttatat ttactgtaat
     5821 atttattttt gcagtacttt atataatttt ttctttttta ttttggattg tggagttgtt
     5881 atctaaaaaa actaaaaatt ataagtttaa tttaatagta aaattatcaa tttcaattat
     5941 tttagcaata ttaattacat atactgtatc taccccaatt tgtgctggtt gtggagggag
     6001 tactccaaca atagcaacga cacaattatt aaaatcttta attgataatc caggagcaga
     6061 aagttgtaca tatcctgtta catttactat atcaaaatcc acactttcag cagaaagcat
     6121 aacaagagat acggggttag agcctgagca agtttatttt gccaatcctg aagacatagt
     6181 aaattttgat gcaattgatt ctcaattact aaaatatact gccacatcaa gcaaaaaagt
     6241 agcaatttgt gctatttgtt cgcaagatgg caaaaccgga ttagagcaag cacttcaaga
     6301 aaacggcata agcaacacaa caataaattc aataatagaa ggagaaacca tttgtgcagt
     6361 ttatccaaga aaatcagcaa attaaatata taagaagctt taaaaagatt ttgttaatat
     6421 tatttcatta atatatttat attaaataga gtaaataaaa aataaaaaag gtgaaaacaa
     6481 attatgttaa aattaacaaa gaaaggacaa gcctcatcaa catttcaatt gttgattgcc
     6541 gcagttgttg ctcttgcaat tttaggtgtt ttattaaata tactaggggt tcttccagga
     6601 ctaaataaag accctacaaa gactacaaaa acacttttaa ctgatcaaat aaataatcca
     6661 ggagcagaaa gctgtacaga cccagttaca tttactagat caaaatcaac actttcagca
     6721 gaaggcatta ccgttaatac cgggatagaa atgacacaag tatattttga taatccagag
     6781 acaatatcca attttgatcc aaaggacagt atattaagat atggagcgag cggctcaaaa
     6841 aaagtaatga tgtgtattgt ttgttcttca gataaaagca cattagatca ggcaattgag
     6901 gcaaatcaaa agacagaccc agcagataat tttaaaactc aacccacaga attatcttct
     6961 ggggaaacag tatgtgtaat atatccaaga aagacatctg cttcataaga taaaatataa
     7021 gaaaaggtaa gatatgaact taaaacaaga gcttaagaga ttatggcttg ataaaagagc
     7081 gcaaggggat ggcgcttcca ccctctatat gcttattatc atagctatag ttgctcttgt
     7141 tctaattgca gtcattaaac ctatgtttaa tagcagtatg aaaacttcaa tgaatgtcgc
     7201 agacttgcct aaccaaggta caactgcgac cccaacagtc aattctacag caccaactac
     7261 tggctcaagc tgatttcttt aaaacaacaa gctttttaaa ttctttttct atatttattt
     7321 ctaagatgaa caaaaacaaa aatatatttt tgataaaaag tagtaaggct caagcaagcg
     7381 cgccttttga agttcttgta gctgttattc ttatgggctt tgttattatg gcaggtacct
     7441 gggctttaat caacctaaac caaagcgttt gtgtaggtaa taaacgacaa agcatgagcg
     7501 aaatgaaagg tataatcgaa gatataataa tgggttctga tttagcagtt aaaaatgtga
     7561 aatttgatat acagccttgt tttaatgaaa aatttgaagc aataacctta aagacctata
     7621 ctaacgaaac actctgtact tcttattgta atgcaggaga aacctgtacc ttaatggaat
     7681 atgtttatga tgatgataaa actacgaaac gacctattgc tcctatgtgt ttaaatctac
     7741 caactgcaat taattatgca agttctgctg aaggttgtga agaaaattct gaggaatggc
     7801 tagctttgaa ttttggtggc agtaatccaa attctatatt aacaagcggc agctataaga
     7861 ttttcatgag aagtcagaca ggaacagatt ctataagaga attgtgtttt ttaaaaaaaa
     7921 ttgattaaaa atgattgagt tttctttatc aaaattaaat ttattaattt ttgtaacagc
     7981 aattgctgca atagtaatat tctttatagc aacatttaat tctagtatga aaacaagaca
     8041 aagctatgaa ttggtttaca aagtgggaaa agaaataaag accgggatag aaaacgaaag
     8101 ctattgtact gtaaaattta taacaatacc aaaaaccata caaacaaatt ctggaaattc
     8161 tgataccttt aatatgagat ataaattaaa tgtttctgca tattctgtag gcgatgattc
     8221 aaaaaggtta gttcttactg taatggatag aaaagaaaaa ccaaaaatct atgctgctta
     8281 cgatattgac tataatggtt cagttgcgtt atatgcatca gaaagctgta caagttttcc
     8341 aaactgtaca attaccaagt cagaaaattt ttctgtagat tttgatccat ctaaaagaga
     8401 ttcgatagac acacaaatac tttttgcaaa gattatttca aatggaaacc ccataatttt
     8461 cttaattccg tgtcttgaaa aaaaaggaat atatacttgc agagaattta taaatacgaa
     8521 agatttaaag acgttgattc cttgtttaaa tatagttagt gatttagtaa attatactga
     8581 taattaggat taaacatgaa taacaaaaaa ggatatattg gcgcaattgg agacgatctc
     8641 ccaagcttaa taccaatatt tgtaggttta actatctttt ttgcagtttt tttaaatacc
     8701 tataatgttt atagaaataa cacagatctt tattccttaa gaaatgatgc aattaatatt
     8761 tcaggaataa taaaagaaaa tccaattatt tcagaccaaa atcaatttat aaaatcttgt
     8821 aacagagtta aaacaaaata caattggaat gcgtttatta taccgttaga tcttaatagt
     8881 gaaaattata acggcttaac tattgatgac ttagaagtag aaaacattat gaaacattgg
     8941 gcagaaaccg aatctcaacc tagaaaatta atatgtgatt ctgagggatt aggggcttta
     9001 agaactgcat tacaaacaaa agatgtaact gtctataaat atccaataac tgttcaacaa
     9061 caattttatg tagaacctgc atggatttac attgttgtgt ggaaatgata taaaatgcaa
     9121 ttaataaaaa taaaaacgag aaaaggtcaa gcagcaataa cagatgcttt atttttctta
     9181 attataattg tcgccctgtc tgtattaatg tttagatatt cttctactta tggcaacaga
     9241 atagaacaag caagttctga tttatattat aaagaatata caaattcggt tctaaaaacc
     9301 atattttatg tatctgcacc tttagatttt gatttaaatc ttgaaaaaac caaacaaaac
     9361 gattatttaa tggctcttgt aaaacaagat tattatgcac agggaaaact tggtttttct
     9421 gatatgaatg atctaaaaac agataattat ttagacattg caaaatacaa tttatttcat
     9481 acaattaaag ccactatgcg tccattagaa tcacaagatt atttgttcta tatttttaat
     9541 aatgaagatt ctaatttttt atattttaca attaagaaca cacactttga aatatcagaa
     9601 gctaatccaa atagtggagt agatgcggtt agaggagtta caagatacat actaacagaa
     9661 aatccatatc attattatct ttgtgatcct gaaagttatg aagatgtaag agctgttgtt
     9721 tctaggtctc caaagatttt ttcctcttct atacctttgt cgtttacata taattatagt
     9781 acttatgcac aacttgggat aaaagaagat attgttactt ccacttttgc aatgtggcca
     9841 gcaacagtcg atataaatag cgatttgctt attccattaa attgtaaaga agttgatccc
     9901 ctatctagca atccataagc catactacca gttaattaat taaaacttca aacctaattc
     9961 gtcatcagaa tctgcgtctt ctgcatcatc atctccaaaa tctaatttta attcttcttt
    10021 tttaggtttt gatgattctt tattctttac tattttatct ttaatattat cttctgattt
    10081 ttttgttgtt tctttacttt tcttttcttt tgcgttttga attgcttttt caactatttt
    10141 ttcttttttt gccttttctc ttatatcttc ttttttattt tcttctacaa catcatctat
    10201 tactttctct agctcttctc tgtcatttaa gaaatcataa ttttctttgg ttttaacagt
    10261 atttgttgaa gtactgccag ttatatttct agaaccccta taatgtgcgt tgctttgaac
    10321 aacttgctta acactttctt gagtaacatt aacatctctt actgataatt gagaatatat
    10381 catttctgcg atttcatcaa tatctggatc aattctgttt tttaggtcta tgagtatttt
    10441 atctataatt ttttgctttt cttcagaatt taatttttta ttttctttta tagattttaa
    10501 tgttttttct ggtatttctc ttgttgattt taattctgtt tctttttttt caggaaccat
    10561 tttttttgta tcttttttag gtttagttat tggtgtgtca ccataaacca tttctccatt
    10621 ttcttctttt ctataaaatc tgcgctctct tctcttaatt ttgttaatat ccataattat
    10681 tcctcttttt tctataaaca tataagtata taatatttaa aaactattat gctaatatat
    10741 atttaactaa aatattataa aaaggcataa ataatgaatg aattattatt aagcacacta
    10801 aattacatat gggtttttat taaattatta ctatttagtg ctttgttggc tctgccattt
    10861 attggtttaa cctatctttt ccagaaaaaa tttaattggt taagacttga taaaaagcta
    10921 agctttgcaa agtctttgtt tattattatt tttgttattt catatattgc tttaataata
    10981 gtttattttg tgccggtatt agataagttc tcaacattta ctttctttga aggcgtctta
    11041 tttgtattga tacaaattgt aaggttattt ttagtaaatt tattatttac tggaatgttt
    11101 tttattattg caatgtttat atcaatgtta tatgattcct ttatggaaaa aaaagaaaaa
    11161 aagaagaaaa caaaaaagca aaaatcagaa aaattagttt ttatagacct ttggaaaagt
    11221 tttgctatgg ttttgtttgt tgttatgtta ttcttaataa taatattccc atcattaatt
    11281 gcaatgattt tatatttgat atttatgtaa ggtgtttttt aaatgaacga atcaattaaa
    11341 agagtttgga tttttttctt tttaatactt ttctgcaata gtgtatttgc ttatggatat
    11401 aatccggata acagctatta ttttgcagaa aaagaaataa gttctgatgg cgttgtcttt
    11461 gatagaggca atggctgtat gactaaagag ttatcagaaa aagaagttaa tgctctttgg
    11521 ggagaaatat taacaaaggg attctctgca gatcttttag attcagatca agaagcaaac
    11581 actaacagag aaaatctaga taaaaataat ttgattgttg cagatgctga tgctaaatca
    11641 ggagatgttg gtgcaaagat gcaagttccc tctcaacaaa tcaaccctct tgaagttcca
    11701 atgttaatag gtaaaacctg ttatgggtct tatgattttg gacttaattt aaatgaaaca
    11761 ttaagagttg gcagatgtga tcttgatagt aatacaagcg attgttatct tagtatgcct
    11821 gggcaataca gacaaaacaa tttaagtgct tcaaatgttg gaggattctt taaagaagta
    11881 aaagttgtag gaaaagattt aatttctgga attaaagaaa cagtaataaa aaatgaagaa
    11941 gatgaaattc ctggtaaaga aaatatgggg ccattttctg atttatcttc acgggagtta
    12001 agtaaaatag aacagaccat gctagcaaac gatgcaaata caataaatac agaaacctgg
    12061 gatgctgtaa gtgatgaagt tctagtaaat agttctttaa ctcaaaattt tgcagcaaca
    12121 atgcaaacta cctgtagtgg agaaaattgt tatattaaca cttattcttt atttgacaaa
    12181 atgtttaatc aatatttttc tgcagatatg gtttacagtt ctgtatctcc atttttattt
    12241 aatggtgccg caaggatatt taatgttcca ggagttaaaa atgcagtctt aaaaccaata
    12301 aatactttaa aagataaagg gctaataaaa gacggcggtt ttttagatac tctaactaaa
    12361 gatccagcat tatttgttaa agaccctttt ggaagaatta gtcaatcaat aaaagcaagc
    12421 aaatatgcaa acgctgacgt agatagattc gtagaattta atctggcgca caatgaaata
    12481 atgggaagag acatagctca ctacaatatg aaaggattta cagataatat gtttgattcg
    12541 gtacaagatg gtattggagc aacaagtaaa tttaataaag aaatgatttt agatggtact
    12601 aaaaacttaa gtgcagcaca aagacgcgta gcaacagatg tagctcagga atatgcagat
    12661 aaactatatt cttctagcgc attattaaaa tcaaaacttg caaatcccat aactagtgat
    12721 gcaataaaca tctataaaaa aggtattgca aatggagcaa atataaatga aatattaagc
    12781 aatttaactg aagaacaata taaagcatta gatgattatg tatatacggc tacaaagctt
    12841 tctaaaagtt tagaaattgc aatggatgga aaatttaaat ggaaagattc tctatttgac
    12901 actgcaacaa taaataataa agttgcaaaa gtatatgata atggtgtaga aagggaaatc
    12961 aaaattgaaa attataaatc attagatggc ggatcaaatg ctttgtataa aaaagaaatt
    13021 ttacaagatg gttcagtaaa gatagatact aaggtttatg gtgatgaatt caaattattt
    13081 gaatatgata gagtaaatca agtctctcaa ataaagggtg ctggtaaatc tgttgtaaca
    13141 gaaaatatga aagttccact tgctggtggg gaagttaata gacaacgact aaaggcggtg
    13201 tataaagatt tagacaagtc tggagcaata aaaataaatg atgcggccgg attaacacca
    13261 tttcaaaaaa acactactgc ttttgtttct tttacagatt cggtttctgg acctcaaact
    13321 atatccattc atgattattt tactaattca tacacccctt tgcctggaac tatagaacta
    13381 tatccaaaag gctataatac aatattaata gatgatgtag aaatttttga tacagtgctg
    13441 ggaaaaaaag taaaaataaa agatgcaatt aatccaaaca ccggaaaacc atatggccca
    13501 gggtattttg atttagatcc tttagaagta gcggctccag ttcttattcc tccgtcttcc
    13561 ggaagtacgg ttgtaaaaaa aatagacgat accgcaaatc gttttagtga cgtcttaaaa
    13621 acaatgagtc ataaagaatg ggtttctggt aaaggaagag acgcaattaa tcagttaatg
    13681 agaaattata atggtggatc ctatcaaagg tttttaacac aaaatctatt agtttttgga
    13741 cttaattttg catactggga agttaaatct ggtggtgcaa caatatttgg agatacaatt
    13801 ggaatgacta gatggtcaat gtatcaatta cctgaaactt atactgcttt aaaaattaag
    13861 cacaaagaaa acggagagat ttataaagac gcatatttag atttctttgc taatactggt
    13921 tcagatcaag gagatctttt catgaaatat ttaaattcta tgttattttg gtctgttaat
    13981 gtaacctcaa aagtagttga aggtgtaggt gccgattggt ctacttctgt tgttgatttc
    14041 ctaaatgcac gattacaagg ccaaattaga agatcagaaa cagacgatat agtattaatt
    14101 acaaataata tttcttcagg ttgtaacaat caattttgta attttacaat cggcgcaaac
    14161 tatattgatc aaaacacaat gaacgcatct ttatctgcta tagagctaga agaattagaa
    14221 aaagaaaata aagatgccaa agaaaataca gaagatgcag atgccaaaga aaatacagaa
    14281 gatgcagatg ccaaagaaaa tacagaagat gcannnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnngatgcta aagaaaatac agaagatgca gatgctaaag aaaatacaga
    14461 agacaaagga ctattagact ttataacaaa aaacaaaaac acgccaaaac taaaaagcca
    14521 agttgattat aaatcaataa gtgttgatac tgttacttct gatggtataa aaacaaataa
    14581 ttatttatta gagaatactt ctgaaaaaaa tttaaaagtt ggacaaaccc ttatttcttt
    14641 ctctcatcat acagactatg atggtacaat ggctggtcaa tctacagaaa aagcaataga
    14701 tttaattgag tcaagagaga aagaagaaac ttgcgggcaa aaggttacaa atttaacttt
    14761 tttaggggcg ccaataggta gcgtcattcc caaatccttt agaaatcaca gatttgttgt
    14821 tggatctatg atagcacaaa aatttgcata tttggttttg ccaggttctg gttattttaa
    14881 ttcatttata ggccctgcaa ttgtaagcga tatccctatg caaactatga ttatgccaga
    14941 aatacatggt tgtattgatg atgaagaagg actttatgct catttctttg tttctgctca
    15001 agaagaagaa agacaaaata aagattctaa aaataaagta ggagaagcaa taattgatgg
    15061 aacaaaaaaa gtcgaagaag ttgtttcaaa agtaacttct ggaacagaac ttgaaaaagg
    15121 agtaaaagct ggttcagata aagtaaaaga atatgttgaa gaaaatatta tcgaagatcc
    15181 aattgttcaa gcaacattta ctactggtgg aggaacaaac acagcagtaa aaggacaact
    15241 tttctttata gagattggtc caaaaacaaa atgtatggca agtggctata atgataaagg
    15301 tgtggaagta ttaaaagata aagatacaaa tatagatgtt acaattgata aagaaaaagg
    15361 aatattaaca gttactgatg aaaatggcac tagaacggtt attggtgaag gtagcaaaga
    15421 ttttgtaaga ctaattggag ataacttagg tattcctgca aaagtcatac ctcgtagttt
    15481 aacttatata ccattaccaa acaatacaga accattattt gaaattgatg ctcatggaaa
    15541 tcttactgta aaaaattcag agttttttga ttgtctaagg gcaggttatg aagcacaaac
    15601 tggccttacc ctgaattcta caagtaagaa cttaactcca tatcttggaa ttgtaaaatt
    15661 aacaaatgat attaatccta ttggggcata tacttctagt tttttaggga attcaattat
    15721 ttctcagggc gctggtcttc caagattaat tgctaatggc aatacttcta aattaactgt
    15781 tttaggaaat agatctacaa caattggccc aatagatgga aaagaaaata gaactggtaa
    15841 aaacataagc gttcagtttg agtttggtca gcttgtttat agtgaagaag caaaagccta
    15901 tattatgtgg gtagaaacaa caacagaaac aagtggtaat gatattgcaa gcatgaaagc
    15961 agaagtcaca aagaccaaag caataaatgg ttgtgaaaat gaagagattg gtattaattt
    16021 ttctacatct ccagtaaaag ataatgatca agcagcagca aacacagaaa aactaaataa
    16081 ggctttagaa aaagttgggc ctttccaaat gtttgatact aagaccaaaa cttttatttt
    16141 ctatgtaagt gatccaccag aatgtgagca aagactaaag ataatagata aagaaactgg
    16201 agaaatctat gatgcaaaga tatcttcttt tgagcaaaca ccaaatggta tttctgtaaa
    16261 aactgatgac ggccaaacac ataatcttga gtttagtgca gaagacggtg ttccaaggct
    16321 tacctataat ggagaaaaag acactttaac ttctgctcaa ggaaagaacg gttctttctg
    16381 gtatgaccca gagacaggta attggtatac tacaaatgga aatctaatac cactaaatga
    16441 tgcatttaga gatggtgttt cctttggtgt tgatggtaat ggtaaagtta ctggaactcc
    16501 aggaactaat gtgtttaata ttggttctgc agggtcaggg aaaggaagcg gaggctttaa
    16561 tattcctttg actcctgaat caacagttat gataattctt tacataacag taatattaag
    16621 tggatttgtt ttaatctata tggacaggaa aaaaagaaaa taaatccatt tatatttatt
    16681 ttttttctta aatatatatt tagatgtgat taaaaatggg aagattagaa gaatatcagg
    16741 aaaacagaaa aaataatttt agttctatag taaatatggg aatagagcct tatccttata
    16801 cttatgatag aacgcatgtt tctaaggata ttaaggaaaa gtataaagat ctagagccag
    16861 ataaatatac aacagagatt gctagtgttg ctggaagagt tctttcttta agatctcttg
    16921 gtaagatttc gtttattgat ttacatgata cttatggtaa gatgcaaata gttgttaaga
    16981 acgagactac aagtcctgaa aatcttgagc ttttaaaaaa gcttgatgat ggtgatataa
    17041 ttggtgttaa aggaaagatc tttaagacaa aaagaggaga gctttcaatt gatgcacagg
    17101 atataacttt cttatcaaaa gcctttatgc ctcttcctga gaaattccat ggtattcagg
    17161 atgctgagct gaaatataga caaagatatc tagacttaat gacttctaaa gaatcaaggg
    17221 atatattttt tattagagca aagattatta agattattag agaaatactt gacaagaagg
    17281 gtttcttaga agtagagaca cctttgcttc agtcccttta tggtggagca aatgccagac
    17341 catttataac tgagagtttt gtatggaaga gacagcttta tctttcaatt tctcctgaac
    17401 tttacttgaa aaggctactt gtaggtggtt ttgacaaggt atatactatt tgtaagaact
    17461 ttagaaacga agggatagat agatctcata atccagagtt tacaatgctt gaatgttacg
    17521 aggcttttaa ggattataat tcagcaatgg atttgattga ggaaatttat gaaaccatag
    17581 ctttagaact taacggcagc acaagggtta cttaccttga gcaaaccttt gatttcaaga
    17641 gaccatggaa gagattaacc atggctggcg ctcttaaaga gtatgcaaat cttgatgttg
    17701 ataaattaag cgatagcgac ttagcaaatc tgcttaaaga aaacagattg gaattagaga
    17761 cctataagag aggcctagcc atctctgagt tgtttgagca tttctgtgag aaccatcttc
    17821 ttgggccagt ccatattatt gaccatccaa aagagactac tcctctttgt aagcttcata
    17881 gaagcaataa ggacttgata gaaagagacg agccatatgt taattactca gaggcaggaa
    17941 acatttacag cgagttaaac gacccaatgt tacaagaaaa gctaatgctt gaccaggcag
    18001 accagggaag aggaaaaggc gagaaccatc cagtagacac tgactttatt gaatccctta
    18061 agtacggaat gccaccagct tatggtcttg gccttggtat tgaccgcctt gtcatgatat
    18121 ttacaaactc cgcaaacata agagaagtaa tattatttcc aatgatgaaa gaagaagggg
    18181 atggcttaga agataacgaa aaaaagtaag gtatttaaat atattagata tacttattat
    18241 tacaatattt aattaaaatt taaaagggga tagaaaaaaa tgagtcaaaa atgtattatt
    18301 tgtggaagag caattggtaa ttatggcata tattgcccaa aatgtcaaga agatcttgaa
    18361 aagaaaatac aaaaaagaga aaagtttgaa gaagtctaca gaagtgctga tttctttgag
    18421 agaatgaaat taaaagaaac taaagatgca ctaaaggcta gacagaatag attagctcaa
    18481 aaacctgttt ctaaaaagta aaatggaaaa accgaggacg gttttttccc aagaaaaatt
    18541 atctagttct caaaaaattt taatttttct gggccataaa attttattaa tttttttggg
    18601 tttttgaact tttttgtcca ataatttgtg aattaattta attggctgtc aagtgcagct
    18661 tttatttctg caattgtttt ctcagaacca caggatctta atatttgatt tccttcttta
    18721 tctagaacaa cataagctgg aacataacgg caatctccca ttatttcttc atatatgata
    18781 ttagaattaa ctccttgtgg catagctact ggaaactctc catctccaat tatttctacc
    18841 catatatttg ctttgtttga ataattattc cagatttctt tttctagttc aggtaaaagc
    18901 ttctgacaat gtccacaata ggttcctgca aacacaataa agcttggttt acttgtagca
    18961 tagaagtctt caagctttga gctttttgtt atttctccag agattattac tggcgtagtt
    19021 tctaaatttg gattttctgt ttcttcatta attccaagtt ctggcattgt ttcttgagta
    19081 tttgtttctg cgttattatt attcaaatat tttaaagaaa aagttattat taaaagcacg
    19141 caaagtattg ctaataccca gatgattatc ttaactctat tatttttaga aaaaaaatta
    19201 gtttttttct tttcttcata tttactaatt tttgtttgta ttttcttttt catattattc
    19261 ttcttttttc ttttttgatc cttttttcat aatataaatt tcaagaggtg gaaaaccatt
    19321 aaagtatata gaactataac ttctagtgta tgcgcctgta gaaaggatgt ataatctatc
    19381 tccttctttt agactctttg gcaaaagata cttatgcttt tcataaagtg tgtctgtaga
    19441 atcacaggta ggtcctgcaa gtattacttc tctgcagtct ttttcctttg gtggctttct
    19501 atagttttct gtaaagatag gatatttgat tgattcatca agtgtttcaa ttagtcctcc
    19561 aaatttacca acatcaagat atacccagtc aaactgatta gataaagact ttttagaaat
    19621 cattacaact tctgtaacta taactcctgc atctgctgca attgaacgac caggctcaat
    19681 tatgatctct aatttcttag agaaatgttc tccaaagtct tcttctaaaa atctagttat
    19741 ttctttagaa tatgtttcta agtcatgtgt tgggcttata tagtttgctg gaaatccccc
    19801 gcccatattt attagcttta attctatccc ttcttgttta acggcatcaa acaaatattt
    19861 acattgtgca attgcttgat cccattggcc tatatctctt tgttgtgaac caacatggaa
    19921 ggatacgcca tagggatgta atcctaattt ctttgcttca aaaataagat gataaatcat
    19981 atctgggtgt gcaccaaatt ttctagatag tggccaatca gcagaaccac cctccataat
    20041 tattctaaag aaaactttag agccaggagc cgcatgtgca agttttttta tatcactaat
    20101 tgaatctgtt gcaaatagtc taatgccttt cttgtaggca taagcaatat cttcttcttt
    20161 tttaattgtg ttaccaaaag atattttttt tggcgatatc tttaatctta ataattgatc
    20221 aagctcgtaa tttgttgcaa catcaaagtt agaacctttt cttgcaagca atttaatgat
    20281 gtcatcttct ggatttgctt taacagcata gtatattttt gcaaacggca tattcttaat
    20341 taattcatcg tatttttctt caatcttctg aagatctata attaagaatg gtgttttttt
    20401 gccttttgca aattttttta tttttgtaaa ctctttcttt gttatataat caaagagtgg
    20461 cctatttgaa aacatatttg ttctttaatc tcctttttat ttagtcatat tttacaatat
    20521 aaaataaaaa aaataaaaaa tgaatttttt gttaatttca attatttctt tttaaataat
    20581 attcttgcta ataaatagct aagcgcccaa agtattgcag taactggaat agttagccat
    20641 aatgcagact tacctaaaac aaaaaatatt tcaataataa gcatagatat aaataatgtt
    20701 gaaaatacta aaaacataac taatgaaaaa taatcccagt atatttcata ttttatattc
    20761 ttttctgttt taaccttttt tatttgtctc ttttttttaa ccattttatt attcctccta
    20821 tctctttttt ctttcttttt gtgtttcttc cattatatct aattgtaatc tttgtatttc
    20881 tagtaatctt tgccattgtt tttgaacaag attatccatt ttttcgtgta aatgtctaat
    20941 ttctaattct gattttaagt ttatcttata atcgttttct gctcttattc tatccttttg
    21001 gtcttttcta ttttgactca tcataattat tggtgcttgt aatgcagcta aacaagacaa
    21061 tattaaattt aatagtataa atggatatgg gtcatatggt ttttgtaata agaaataact
    21121 atttattcct atccaaataa taagaaaaac accaaaggaa ataataaacc cccaagaacc
    21181 cccaaacttt gcaacaccgt ctgcaagttt ttgacccact gttagatttt ctttttcttg
    21241 cattatgtct ttaactatgg attcgtgttc tacaaggctt ttaacaacct gtttatcaat
    21301 tttatttagt tctccttttt ctttcttaag aatgctttca atgtattctt gtcttgcaat
    21361 ttttaattct tctctagtga taaatccgtc ttctgttgct ttctggtttt tttttctaat
    21421 aattgctaaa agttctgatc tgactgtggc gataggtatg tctccagtta caccatttcc
    21481 atttttatca gccatatata ttcaaattat ttttttggtt tagaaaatct tattaatacg
    21541 cagattgccg caatagcaat tatgctattt ataatagtgt tagctatgag ccctactttg
    21601 taaccgtcta ttactaataa gattgttcca ataatgtatg caataataaa cacagtagat
    21661 atttctcttc ttttattcat agaaaagaat tgaaataacc aagcaataaa tattattgct
    21721 aaaccaataa tcgcaagatt taatgtcatt tctttgcctt ctttttagtt ttaattatat
    21781 tagttgtttt ttttttaatt gtagctactt ttgtagtttt cttttttgca aatttctcac
    21841 agccacctgt tgcaacaaca tctctaccaa cgcaagtgcc tttatttttg tttgtaacat
    21901 ttggtctaaa tcttaaacat tttgaacagg ttttatctcc tgcgccctca ctccaatatc
    21961 ctggaaacat ttttttgctc ctttttaaaa ttgtattttt ttctatttat atatagatat
    22021 attatattta tatatctttt gttaggattt ctcctaattt ttatttcgtt ttgtcatcca
    22081 ataggttatc caaactgcaa ttatagtaac taaaaccgca taaataatca ttgcccagac
    22141 tgtctgagct gttccaaata ctctttggaa tatggtttta atggtttcat tccaggcaag
    22201 agctgcgaca agactaaatg ccccagtaat caatgttatc aatttttcat taacttcttt
    22261 tctcatttgt tcttgttctt tcttaaattg agtttgttgt ctttttagtt tgttcttcat
    22321 tctcttaagt tcttcttctg ttaatttaat tttagtaatc ttttttgtca tctttagtat
    22381 ttttttcata taatcaccaa tataaaaaat tatagtctat tgttttaaaa tactttctgc
    22441 ggtattattt attatatgaa aacaccaata atgcctacaa ttaattataa aaaattttca
    22501 tggaatgaat tagataaaat agatcaaaat actataacac aaatttcaga aattgacaat
    22561 ttattttatc gatcctcttc agatttagaa gaatatctac gtaaaataaa aataaatgca
    22621 gataaaacaa taatttattt gtgttatagg gggaaagaac tggtgggtgc agtttttggt
    22681 actttaaatc gtgtttctga attttgcaat aaagattata aaattaaagc aaaacctaaa
    22741 gactatatgt ttttattaac cgagtttttt gttaagcctg gatttcaaaa taatggcatc
    22801 ggtacagcac taatggctag agtatttgca gatcaaagag ccaaatatga ttctaaattt
    22861 atatatatga aagcatttcg aaaaacccaa actataaata aaaaactaac cggaataacg
    22921 ccatttattg tgcctataaa agaggtggca gaaattaaaa gaatagtaaa taatctttca
    22981 ataattgaaa aacgtaaaaa aagaaacata tcacataata aatcaatgaa aactggcgcc
    23041 ttaaaacgga agatatttcg taattttaaa aattcaaaat ataaatatga tttaatagag
    23101 acctctaatc catcaattac tgatgttaaa gatcattaca tcgtaataaa taaaaatcca
    23161 aaatataaaa gaaagcaaat aagaagtatt gcaaaataat tttattctat ttcttttctt
    23221 tgcttgtttt tctatatata aatattacaa gtaaaaattc tgcaataatt actactggta
    23281 gcatcttatt caataacttc gttgcagaga ttgttgcaac aaataaagat acaagcgatg
    23341 ctgaaagtat agagtatctt ataattttat ctttaattaa tataaaggcc attaacaaag
    23401 ccaaaagaaa agtaaaaatc attctgccaa tatttattct attattatat gtttctaata
    23461 tcttgttata atctctataa caatagctac attccatttg gcagtctttc ttataaacaa
    23521 tgttcccttc tgacttataa caagaattaa tgtattcttg attataggaa caattagcat
    23581 ctatatttgt aataacttct ggtttgtaat tataatcctg taaataacaa ttatcttttg
    23641 gtctttctaa aaataattta tctattgtaa aaacaaaact aataactaaa gcaaaaatca
    23701 aagtaaacat tataatatta tatactacat tttctagttt ttttgatttt acttttttat
    23761 tcattatctt ctacctctat tggtgtattt tttttgtctt atttttttct tatgtttaaa
    23821 aaaatccatt cttttctttt taatatcttc ttctgttagt tctccaatta ccatcttttt
    23881 ttggttttct ttttttctat cctttctatc cttactgaaa tgttctcttc tttcttgata
    23941 tctgtttatt ctttcaagtg ctctttcttc ttcttttgtt ctttttagag ttataggaac
    24001 aattgacggc aagacctttg cttcaactat aaaaccaaat ttcttacaca ttcttagaaa
    24061 gtcttcttct tctttttgag taatgattga tattgctctt cctttctttc cggctcttgc
    24121 tgttcttcca attctatgta tatattgtgt attgtctttt ggcaagtcgt aattgtagat
    24181 atattctaga ttatcaatgt gtatacctct tgcagaaaca tctgtagata ttaataaccc
    24241 gtctgcattt gcgtgatatt tttcaatggt tcctgatctt ttattttgtt ctaggccgcc
    24301 atgtaattta aagcattcta ctctttgttc ttttagatta ttataaataa cgtctacctc
    24361 gcttctagta ttacaaaaaa caatagcttt tttactgtgt tgcttaacaa gatgtaaaag
    24421 caaagaaaac ttctgatttt gtttgactag ataatagtct tgtaaaagat tttttgggtc
    24481 aacgtaagtc tgcacaacaa ttctttgtgc atgagagata tattttcttt cgatatttga
    24541 gacatcgttt gttatagttg cagaaaaaag aagcgtttgt ctttcttttg gtagttgctc
    24601 aataattctt gagatatctt ctgaaaaacc catgtcacac attctgtctg cttcgtctaa
    24661 tattaaatat ttaatattat taacttttag atttcttctt tcaaactggt caattattct
    24721 tccgggcgtt cctataacaa tgtctgcata ttttaattgt cttgcctgat taccaacaga
    24781 agcgccgcca aatatagcac aggcttgaag ttctttaact tttgctattt ttttggtttc
    24841 ttcataaact tgattagcaa gttctcttgt aggtactata attagtaatt gaactttttt
    24901 ttgttttgta attgatggta aaacacctgc aagaaaagca atagtttttc ctgtacctgt
    24961 tgctgattga gcaattatgt ttttgccttc aagtattgca ggaataacct tttcttgtat
    25021 ttctgttggc gtttttatgc caatattatg tattgcctgt agtatttgct tatctttgac
    25081 aaggccttca aagttattat ttgtatttgt catgttttat ttttcccatt tatttttttt
    25141 tataatttac ggggtcaagt ccatattctt ttctgtatct attatatctt gtaattgctt
    25201 cattaagttc ttttgtcgca gcctccatat ttagttgtgg ctgtggatta attcgtggat
    25261 tactagataa atataaagag tatttcctca tatattcttt taatctttga tcaactattt
    25321 tttccagatt ttttaatatc gctttttcgt ttggtttaag tttatatttt tcttttattt
    25381 tattttttat ttttctggta gtttttttta taatacgcat agttcctcac tttatttaaa
    25441 ataataagaa acaaatagtt tataaattaa gattaaaata aaaaaagaaa aagcataatt
    25501 tatatgcttt tgaactttat tatagcccaa attgctgcaa gtcctacaag tgtataaact
    25561 attgttacta atataggtat tgcaccgaat actgtagtta caagattaaa actaaaaagt
    25621 cctacaagta accagtttaa accgcctaca attattaaga tgagtgcaat taaacttatt
    25681 gtgtctctct ttcctgccat ttttataacc tccgtgttat atgttatatt aatatttata
    25741 ataagaaatg tttaaatacc ctaataaata gactgcttga ttcaaattaa ttagttaata
    25801 ttgcatttaa tctgtctgtt atttcttttt tataatctct aaattgcatt attgcttttc
    25861 cttctttaga tatttcaata gttaaagctt ctagttcttt tgggaaatcg tgtttaaatg
    25921 cctttgtata ctttatacct gaaagtcttt gaaacaatgc tgcaataact atgtcgtgtg
    25981 ttatgttttc aaaaataatt gttctgtttg tcattttgtt tactctgttt ggaagtttaa
    26041 atctgtcttt tataatttga tctgctatta cttctggttt agggacaatt ctctttggaa
    26101 agtttccatt taaccaaatt ttaataacaa ttgaatcttt tactcctaat ttttcagaaa
    26161 ctcttttaaa aaaagttaag agattatctc tttttgtggt ttttattctt tcgccagaga
    26221 aatatttatg tcctcttagt tctttattta ttcttaattt atattctgta tttgttagtt
    26281 tctctaagat ctctgcagtt tcaatagttc tattttctgt agaagaataa atttttgcag
    26341 gtttagattc tcttgctaat ttaacaatat cttcttttcc tttaggtgtt atgtttttgc
    26401 cattttttgt tttttgagca tgtcttctaa aagatataga tactggtggc ataaaagaaa
    26461 tagaaacaaa aagaatataa atttaattaa aatcaatttt tgcttttatt aaaattaaac
    26521 actctatctg caatctgttc tccatagata gattgtattt ctttataaga aaagccagca
    26581 aatcttagtg tttttgggtt tattcctctt gcagttagta tttttgtggt aatttttggc
    26641 ttttttaatg cctctgaaac taagaatcta atttctaaaa cttttgttct agaatatttt
    26701 ggaggatatc ttattgcgcc tttatatgac cacacaccct ctccatttag tgttgcatta
    26761 tatgatttga tgtcttgacc tgacaatttt cgtcttaaag ttctattgtt atttgcttta
    26821 gtttctgttg tatatccaaa tggcttacca tctgatttaa aaatatctgg tcccatactt
    26881 tggttacttt ttataagccc tcttttaagt ttaaagtcat attctccttt ttgctttaga
    26941 atttgggctc ttttaaatgc tctatagttc tttgcgcctt tttctgttga aaatacaggt
    27001 tttcccccca aggtagcatt agggccgata cttatatttc ttttaataat tccttttctt
    27061 actatttttg gttttactac aggtcttggt cttgttacag gcatttaaca tcatctcctt
    27121 taattattga atttaattta tataaaaggg tagaaggtaa tatttaaaaa gattgcctct
    27181 ttcctgtaat cttggatgta ttactcagaa atgttaaaaa atagcgagta atacataaaa
    27241 acacactaat attaaaaaaa ggaattatct tataataaat ctaagaattc atcttttgaa
    27301 agtcttaagt cctgtttaat tattttattc aaaagtcctt cagggacggg cttattagaa
    27361 tgcattggta taacaactaa tctgccatca gaatgagaaa atattttgtg agatcctttt
    27421 tgccttattt ctttaaaacc caactttatt atgactttag ttagctttaa agaagaaaca
    27481 ataggaagct cactcataat gtaaccctta tcatacctaa aacattatta ttcaatattt
    27541 ttgatttttc ttctttttta acttctaaat atagagaaat tgcttctttc atatttgtaa
    27601 ttaattcttc tacagtatct ccagtagaat aacatcctgg cagattagga acagaaccat
    27661 aatattgttt agtttcttta tcttcttcaa ttaaaacatc aaaagagtta tttaacatat
    27721 cttaccacct aatataatat agaaataatc aaaatttaaa gctttctttt tctaaaagaa
    27781 gcatagaaat aggagtcgga ttccctcctc ccagattcgg ttggggagga acgagttatt
    27841 ggttcattgt tagaagttgg tggatacttc tctttcattc ttaccaactc cttttagttt
    27901 atattaatat tctgattgaa agcttttata aaagtgtagc ttgtgtaggg agtgtataca
    27961 aactcaagtt atctgcacac tacatttatt attttttgaa tatcagcata tttttcataa
    28021 tcgcaaatta atttcccctt attaaattca gaagaattac taaaccataa attaaccaca
    28081 cctttagaat aacacgcaca aattaaagta tacagttgat tagattgata atataattct
    28141 gatttcttac gataaagtct atcaacatct ttatctccac tggtaaactc taaagcaata
    28201 gggggatgaa ctgtttttaa tttacacata tcttctaatt cttcattact tcttgatgct
    28261 ttatttatat atattttata ttggtctata tctgaagtaa aataatcata taatttgtgt
    28321 ttgtctttaa gtaaagcaat tatttcgatt aatttccccc ttatactttt tttaagttct
    28381 atttctttat tatattcata tattttagta gataattttg ctctttcttc ttctaatttg
    28441 agtagcttat tttgagaagg tgtgtttttg gaggtgtcat aataaaatgt attactacat
    28501 tctccacgca atttatttac ttgctcatct attttgttgt attctttaat atctgggcat
    28561 tcttctaagt atatagataa aggagcataa tttgtggtat agaataaatc tatttttgtt
    28621 attagttttt ctttttttcc ttctgtaatt gcaggaaata tacttttgga atcttgttct
    28681 ttaatattat cacttaaagt atccaaaacc aattttataa aatcaatagg atatttctga
    28741 tttaataaat caaatatatc ataaaattca tttaaattta aagttctatg caaaattata
    28801 ttttgcgcta aatcaaatct atttttttta attagatttt tagcataaag tatttgattt
    28861 tcatcaaatg ctcgtttata gtaataaatc atatcatttc catttaccag aatatctaaa
    28921 tcaaaagctt tcttgaattt agttgctttc aatttatcaa atgtgtcctt atctgttgcc
    28981 ttaaaccaac gttcaattat atctctcgca cgttcttctg aaatatcatt agtgctatta
    29041 aactttgaga ttatcaaatc agataaatca tttttcgtac aataagtctt ttcttttatt
    29101 gttttttcaa taaaagggta tatttcttct tttgcatcca tacattccac ctttatttgc
    29161 ttccaaacta ctataaaagt ctggagtatt tttattatta ttaaataatt acgaccctaa
    29221 atgcttataa atagtgcgtt ttattggagc gataaaccac tcctatgctg tttaaataag
    29281 cagttaaaaa tcgtttagtg caatattatg taataatacc agaaaacaac aaaaaggaga
    29341 ataagaaata tgtcagaaag aaaatcgata gaagggacga tattatcaat atcaaataaa
    29401 gtcaatccaa agggggtgcc atattcaata tataagattt ctactcttga aggaaaagag
    29461 ttagacttgt ttcaatggaa taacaatttt agattattga ataagttatg tagatttgaa
    29521 atagaatcca atacaaaaga taacaaagag tataactcaa taattgaaat caaagaggtg
    29581 aagaaagaat gaaagtatat gaacttaagg atacgacccc gtatcctaaa cttaaacacg
    29641 ataatactgt ttttgaatta aagacacaaa gcgattttga acaggcaatc ttggacaatg
    29701 atggggaaat ttttatagaa gatactaaat gttatcttct aactaaaaat aataggtgtt
    29761 atgtattgtt ttacaaagat aaaggaccaa tagtaagaat gttgaaagat agaatggatg
    29821 gggggaacag caatgacaac aatagaagaa atattagcaa gtaatcctac tcagtattct
    29881 aatttatcta tgggaatcaa agatggttta ttctattatg gtaaaatggt tgaacataaa
    29941 ggacaactat tagagatgat ggtcttgtct aatagacaac tcttaactaa ttacagtagt
    30001 ttaaacacaa aaacagataa aagacctaat gaaataaata attttggtat attctatcaa
    30061 gatttaccaa taaacattgc caataacgac tggtccaacg atagcattac caaatttatt
    30121 agttctaata ataaattaat gaacaaaaaa gaagtttaca ataaagtaaa agaacagata
    30181 actaaatata tggatttata tgatgaaaga ataagttcta tggttgcttg ttggtgtatg
    30241 ggcacatata tctataatct attttcagga tatgggtata tactgcttac aagccagaag
    30301 gattcaggaa aaagtaaatt actaaaaatc attggacaac tttcaagaaa tagtatctat
    30361 ctaagttctc caagcgagtc tgttttattt agaatgataa atggaaatag acctacttta
    30421 cttcttgatg attttgaaac attaaagaac gaagaaagaa cacactatat cttgcaaata
    30481 cttaagattg gtatagatga agatggggca attccaagaa atgaaaagta taaggatact
    30541 tttgttccaa aaaactataa ttgtttttgt cctaaagcat taacaaatac ttgctctctt
    30601 gaccctatta ccataagtcg ttgtataact attaatttac ttccaacgaa tacagaaaag
    30661 ggtaaactaa aaccaaataa aagagattta atctggcaag aaataagaga tgatttgcat
    30721 atattgataa tggagaatta tcaagaaata attgatatat acaataacga atcttttgga
    30781 gactttaatt caagacaatt agataccact aaaggattgt tatctattgc aaagtttatt
    30841 gatgattcag tttatcaaga agtttctgaa tttctaaaag aatcttttaa agatagagac
    30901 ctacaagacc tcagttcaga ttgggaatac ttattattta agcaacttta tgaaaaagtg
    30961 ccaagtccaa gatggtgttc tacaaaagag atgaatgaat ggttaaaaga ggtcgcaatt
    31021 cctacagatt ataataaagg atttaatcaa tatattggtt ttgttttgag taaattatct
    31081 tcattattcc caaaacaaag acataatgat ggaattatgt atttgatatc tccaaaaagt
    31141 gtccaagaat atatgagttt aaagaagtat ccttgcacct tagagggttc acaggataca
    31201 caacctacac ttgatttcaa agatgaacca tttagggaat taaaaaatga aattatggat
    31261 acttgtgtaa attgtaatca aagaacctac gttaaatata cttctaaagt agtaaagggt
    31321 agaaacattt gtgaagactg tgtaaagagt gaagccaaaa ggaagggtaa agaactatta
    31381 gatagtatgt attgattttt caaaaacgaa cttaggagat taatatggca agtacaaaag
    31441 aaggtaaaga gataagcaag atgaatgctg taaaaaatgg gttatactta aagtttacag
    31501 acttttttcc ctgtaattta tgtattaaca gagagaaatg ttctgatttt attcctggag
    31561 gttcttgctc tattgattct gaattgttca atcaattagt tactactgag atagatgaat
    31621 taaaagtatt agaccaattg attaactaca atatggttag actatcaaga gcaacagaac
    31681 aatcaagaaa cagaccagac catatcgaat taagtaggat atctgcagaa ataagaaatc
    31741 ttattcaagc aaggtttata ataaaaagaa ggatagaagg tgaaaaagaa tatgcacgaa
    31801 ctaaagtatt gcaaccaatg tccgaccaga gccaattgca tttatttcaa gaaaaataat
    31861 ttctgtgaaa gagaccaaat gattagtgaa gacatttatg gattcaaaaa aaccagcata
    31921 gacaaatggt attctttagt taatttagat agaactgaag ataaaaataa agccaaattc
    31981 tataagatgg tcaataaaga agtaaaagaa cttatacaag atatacaaga gcagatagca
    32041 gaagaaaaag aaaacgagga tgaaaaaaat gcaagacttt aaaaaattaa aaatttggca
    32101 gttaggaaga gagtttacta aagatattta tgatattaca gaacacttcc caaaacacga
    32161 gtttcattca atgaccaacc agctcagaag agcatcttac tcaatacctt cgaacattgc
    32221 tgaaggttgt gggaaaagta gtcaaaaaga atttggaaga tatctttcta ttgttagtgg
    32281 gtcgataaaa gaatgtgaat gctttcttat tctttcaaaa gacttagaat atataactaa
    32341 caaagaattt aatgaattaa atggaaaatt agaaataatt ggaaagatgt gtaataaact
    32401 attaataaaa ataaattaac tatttacata ttgttttata taagtataat ctgattttat
    32461 tttgccaaat tctttatctg catttttcca atctggtggg caatatcggt atgattcgct
    32521 tattgttctt aaatgtctat atgctttata gatttgatta gcatttttac aaataaattc
    32581 ttctctttct ggatgagttc ctggcttttg taaattagga aatttattaa gcacaaaaaa
    32641 atttacccaa tggactgcag aataaaacaa aattattgat gaccaagcct tacaattaga
    32701 ttcggaatct ttagcaattt gttcattgtg ttttgcaagg tctgaaaaat catatttatt
    32761 caatattatc actatttaat tagtgctttc gcattttctg aaatttggtg tataaaataa
    32821 gagttatccg aaaaaacatt aaattcaaat tttatttttt cgtctttaaa tctttccctt
    32881 aattctaatt ctaagtcata aatcttatct ctactatcat aatcgtttgg gtcaattaat
    32941 gtccaaaatc taattacatt tctatcattc ataacataca atttttttac aaatggtaat
    33001 gaactaacta aatgaaaata aaatttaatt gcatatacct ttgtaataat agtaattgga
    33061 ttatataatt gtttatttat cccaatataa cactcatcta ttaatcccaa agtatttgtg
    33121 tttgcttgtt tattaatata catataatcc ctattttgtt tgtgctttca aatctaatgt
    33181 ttttatttgt tcagatattt ttactaaatt ctctgatgct aatttaagtt tatctgcata
    33241 atcttttttc tcagataatg acaaattaag taagttctta tcaatagtag ccatctttaa
    33301 attgaaaatt aaatctttat atatctctga aagttctgga gaatcgtttt ttattagtga
    33361 aattaataat tcttcatcat ttaatacttc gctcattttc ttaagctttt cagaatcatt
    33421 agattctaat atataaactt cattaataaa gctactaatt atttttaata atttagaata
    33481 tgcagtaata aaatcaataa gtatttcttt gtccatatgt aatctatata gttattaaat
    33541 aagttaataa atataactat ttttaaaggc aatttacccc tactatatat atagtatata
    33601 tagaaaaata tataaataga aaattaacac attataattt actttcataa ctctgagtta
    33661 taatacccat tatataatca atatctaaat aatttatttg cactttcaac taaatcaatt
    33721 tttcttctat ccaattccaa tatttcattt atctttttag tattttcatc aataatcctt
    33781 tggtcaaata atgggatttc tatgtctttt aaacgctcta agtttaaatg aggaattgtt
    33841 gatgctatta ctgttcttct taaagattga tatagtccaa tttctttagt aattaacacc
    33901 gaaaagatat attctggagt tattgtaagt ttgtattttt ctttaatata agatatacaa
    33961 cttttcttta atctacattt taaaattcct gaagaaatta agaaattagt atttgtagaa
    34021 gcaattgcaa acatccctat tttcccatct ttagtaaaca atatatcttt ttcctttatt
    34081 tcgaaattta tttgattaaa taaatcttta gaaagatata aatcagcagt tctattaatt
    34141 gtataattta ctaaatctga ggttctaata aatggaatat ctgtttcttt tttaaagata
    34201 tattcaatat aattatcact gccaatttcg ataccttttt taaaatcaaa tagttctcca
    34261 agagtaattg ttggaatccc cttagatttc aaattttgaa ttggatatat atatcttggg
    34321 aataaatatt taggaatcca taaatcataa tcttttattt gagagaatgt aatattaaag
    34381 tagctatctt ttgatttata ttttttaggt tcttttttcc aaagtttata ttcctctgat
    34441 acattcttta tgtcgtcgat ttcattttct ttgcctcttc tatcgtgccc acaattttct
    34501 ataactgaca taaatatggg gtaatctttt ggaattttat ttaatggttg cttctctaat
    34561 attaaaattg ttgttttggt agaggtattt ggcataaatg tttccaatgg aacatcaatt
    34621 atagcattta tttttgtgtg ttcacaaata tattgcctca cataacctaa gctttcatta
    34681 ccgaatattc catcaggtaa tacaatcgcc atttgtcctc catccttaag taattgcaaa
    34741 catctctcca caaaaagtat ttgaggtgct tctttatctt ttaatttacc gatttcccac
    34801 ttattattta atttccattt gtgacctaat gcatattgtt ttaatttatc ttctccacga
    34861 acaggaatct tacttccaaa tggtggatta gttaaaagaa tactaaaatc ccccaaatga
    34921 acttttgttt ttgtggtggt attccaatga tttggatttt ctaaactatc ttcacagaat
    34981 attccacttt taccatctcc aattattgcc atatatgctt ttgcaacttt cgataaaaaa
    35041 tagtccttat ctattcctct tattctatta agtgcaactt ccattttttc ttcttgaaga
    35101 ttatttttat tccaatgata cttttctcct tcagcatcta atttcttcca aacgtgtctt
    35161 aaagattcta tcaaaaagcc accacttcca caagcggggt ctatgattaa atcttcatca
    35221 ttcgggtcta agatatctac catcattttt actacattac gtggagtgaa gaattgacct
    35281 tgccctcctt ttaatgcgtg cccaataaat gtttcaaaag catctgcaat tatatctctc
    35341 tctgcttcaa taagacaata attctgtaac tctcctacta cataagcaac agaattagaa
    35401 tctaatgtta ttgtatcgtt atcatccaat acttctttat attttctttt taccttatta
    35461 aataaatcta aaattctttt tttaacggtt tctgtatctt catccactcc tgctctaaat
    35521 gtgacaattt cattaggttc tgtaaatctt tcatcataaa ttttacagaa aatcaaatta
    35581 ataagttgct gagccaatac ttcatctctt gttgctccaa tggtattagc aaataaatga
    35641 tttcttattg ctttaaatgt tgcttttaag ttgtgagtgg gctttaaatc tttacgttta
    35701 aatctaccta tatcttcaat tctttgtcca tatcgaggaa tattgggtat ttcttcaaat
    35761 tcaattttac ctgatttttc aatttttctt aaaaataatc tttcgtctcc attaaaccaa
    35821 actccaagat atgctttaga gaatcttaaa taatcttgaa gctgagtttt cccatctttt
    35881 ctattttttt tcttacactc tacaataata taaatttcat cttcttgttt tttactattt
    35941 gggaaaattg caatatctat gggatattct ttttttgtat ctgatggtct aattttcact
    36001 ctaaattgtg gtctggtttg gatatttact ttgggatacc cataatcttc aaccaattgt
    36061 ttagaaaatg tttgtaccgc atctatttct tctggtgttg ctttaacttt tatccctgaa
    36121 atataatcct ctatatattc tgccattcat tcatccccat attaaaatta tactatatta
    36181 tagaacaaaa gagaataaaa aggtaaccat ttggtaacct aatttgaact tgatttctta
    36241 aaataaaaca atcttattct ataggtattg ctatgaaaaa agacattgca gaattagtat
    36301 cttttattgt aagagggtct aatagaataa ttatacttaa ttgtttagct ttagaagaac
    36361 taacgccttc tcaaataaaa aagaaaacaa acctatatct tagtcatatc tcaagagtta
    36421 tacaagagct tttggataga aaactaatta tttgtattaa tccagattct ccgatggtta
    36481 aattttatgc tataactaaa aaaggtaaaa ctattataga attagtgagg aaatataaaa
    36541 aagagtagga tattacttag aagtagtatg catttttagt ttttgtatct ctaataaata
    36601 ttctattgta gattccacat atgaaaaata taattttaac acattataat ctatgtcttc
    36661 ttcttgttta tggtctttat cctttagttt cttgtatatt ctggtatata ctaaatattc
    36721 aattgtgctt tctatctctt gagaaattat cattttcttt tgaatcttcc tcatattatc
    36781 ttcaggttta tgtaatccac aagactcatc tgttgttttc ttggaaaact cttcaaaaaa
    36841 atgttctatt actccaatta atttgtgtat ggtcttttct ttatccccca tttctaaata
    36901 tctatagcct tctattaaaa gttcagacac tttttcatat ccatattctg ataatttatt
    36961 agaaattgga acattaatat aatattctaa tgattttggt tcggaaataa ctgtagttga
    37021 aattatagtt gataattcat ttaaaaattt aatattctca aataatattt tagaatactc
    37081 gaaattagat atatctgatt tacgatttaa ttgagtaatt tgaaaataaa ttactggcat
    37141 tgatatgact tctattatta tcctggattc tttactatcc cacttaaaaa gtaaatcaat
    37201 attatatggc aattcagctc ttataccttt taaccaatta taatatagtt tttgttcatc
    37261 agatatggtt tttactttta ataaagattt aagtttatat ttcaaatctg gagtatatac
    37321 tcttttcata ctataatgta taactgtaga gaataacttg ggattagaat ggattttgct
    37381 ataactatgt tctaaaacat ctaccatagt aatatcatca gtaagataac ccactttttc
    37441 catataattc tttaattcta attgaattcc attatataat tctgcaaatt tttggtcaga
    37501 agtatcttta caaggatatc caaagattcc tttaactata aggttattta catttaccat
    37561 atttatcact aaatatatat taatatcgta aatttattaa atagattatt aaagacaaac
    37621 taaatcatac aatccctcca agatttaata tagcactatc taaatctctt ccacttaagt
    37681 gaacatatct acttgccata tcactggctt gtgtccatcc aaagaattgt tttaattgtg
    37741 cttctgttaa atggtttgca aggtatgttg ctctgctgtg cctgaaaaga tgaggatttg
    37801 attttttttc tatattgctt ttcttgaaag tttcattaag tgtctttctt actacagaat
    37861 aacttggtat ttcttttggt cctctgcctt gttgatttct ccttatcatc ttacaccata
    37921 cattgctttc actgtcttca ccaaatggat gttcttttat ccattcttta agatagggaa
    37981 ctgatttgat tattggaatt cttctttgtc ccgtttttcc attaactaaa atagaggata
    38041 tcttttctcc aaaggttatg tgcttaattt taagtgtaag catctctcca attcttgctc
    38101 ctgactcata caataagtaa ataaatgctt tgtctcttgt aaactttgca gtagcaacta
    38161 atttcaaaac ttcttcttcg gtcaataact cttcaggtaa aatattactt ttagaatcct
    38221 taactttaat ccaagaaaca atctttggag cagtttcatc atctccaagt agccacttat
    38281 agaatctctt tagaattatc ttatagtctg atttagtcca aaaagcatag tctctttgct
    38341 caatagtaaa catcaaatat tcaatatctt gctttgtggc tatagcaaaa ggtatttgta
    38401 ttacattaga catatctcta agcatataga tagacttaga tagtctaatt gacttaattc
    38461 cttgagcttt catagatata acatagtcaa gtattgcatt tctgttctct tcaacaacct
    38521 ctaaattgtt ttgtagagta aaaatagctc tgcttagtct gtgttcagtc ctgtatatgt
    38581 cgtcgggacc acttacatta caaacttcta acaaagaact ctttcgagtg cattccctcc
    38641 tcccagattc ggactgggga tctaccggtc actgcctttt tctgtc
//
LOCUS       DAOOON010000006        36745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_1329_length_36745_cov_16.054075, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000006 DAOOON010000000
VERSION     DAOOON010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 36745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36745
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_36745_cov_16.054075"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..337
                     /locus_tag="PLK55_01635"
     CDS             41..337
                     /locus_tag="PLK55_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35670.1"
                     /translation="MNKKKPTKITKEKFSPTLNTILMVEKTLQEMPDSVVKVSELKRL
                     LPKQINHNTLKNILEYLQESNKIFIGVKGIVWIHNDSPVLRKAIADAERNGRTL"
     gene            334..687
                     /locus_tag="PLK55_01640"
     CDS             334..687
                     /locus_tag="PLK55_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35671.1"
                     /translation="MTKKVTVLYSKPVEETLTFLEQTKENNKISKSIYNSFLRKIELI
                     KSNPGYGDNIPKPQIPKEYIEKYKINNLFRLELANYWRCLYSLTDKKENTVEIIAFVI
                     DLLDHNKYNKKFKYK"
     gene            795..1505
                     /locus_tag="PLK55_01645"
     CDS             795..1505
                     /locus_tag="PLK55_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35672.1"
                     /translation="MPGAPKPKVKYEKPTIKKRIATAAAVGLLGGSIALGLHAIEPLR
                     ASSQRSDVKTAIAQIDKKKADVGSRPVEYYSKNPNLIRETVNSYNQKAKLGQIFFYDG
                     KKIKSVYFDQTRLNQITSIIPKSEKAKIDSLISRKIEKVEATAKQNVVLGKKIIDRVG
                     ELDYTKYLLSLTPRELKNAFPEAEIAVRKALERSNPEIFKKINAEKKGGVLFSMIVNT
                     IWGSLWGGAFLAKGKKIK"
     gene            complement(1511..1654)
                     /locus_tag="PLK55_01650"
     CDS             complement(1511..1654)
                     /locus_tag="PLK55_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35673.1"
                     /translation="MAYLELEDIINSLDREAKKEKSLRNKCLYDSAIKALNELKVRKI
                     NNK"
     gene            complement(1731..2123)
                     /gene="hjc"
                     /locus_tag="PLK55_01655"
     CDS             complement(1731..2123)
                     /gene="hjc"
                     /locus_tag="PLK55_01655"
                     /inference="COORDINATES: protein motif:HMM:NF040854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase Hjc"
                     /protein_id="HOZ35674.1"
                     /translation="MKNKQKGSNAERELLFMLFNKGFAVSRVAGSGSSQLPTPDVLGF
                     KNGKALAIECKTKKGEYLNIRDEQVEELKLWEKLSGLKAYVAWRLGKDAWFFIRVDQL
                     NKTKKAYAIKKEEIQAKGKGFDDFISDF"
     gene            2287..2508
                     /locus_tag="PLK55_01660"
     CDS             2287..2508
                     /locus_tag="PLK55_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOZ35675.1"
                     /translation="MFEKNQVFSLQVICNSDRQEIQKIDDNTYKLKIKSIAQKGKANK
                     EIFEFFKDKGLKIEIIKGEKSNKKMLKIL"
     gene            2579..4369
                     /gene="dnaG"
                     /locus_tag="PLK55_01665"
     CDS             2579..4369
                     /gene="dnaG"
                     /locus_tag="PLK55_01665"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF003108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HOZ35676.1"
                     /translation="MGKTYIETIKYMIVADFEVNGLVERPDVIGAIFGQSEGLLGANL
                     DIKELQQNGKIGRIEIEYTQNQNKVRGVLKLPCSVSMVETAIIAASIETIEKVGPCDA
                     VFKVKQIEDTRGLKRQQIKERAIELLGRLMQDEIPDTKELMDEIMDTFKTKDIISYGS
                     EKLAAGPTIDSSKELIVVEGRADVLNLLRYGFTNVVATGGARIPRSLEEISRQKSVTI
                     FLDGDHGADIQQQQLLKSIKVDYIARAPDGKEVEELTQKEINQALRRRIKSNYVSSSN
                     NQFQSQQQFNPNLTKQAGIVKATKFSTTNDHKIANPKFFENKPNKYLQTQQPTNRPMH
                     DQNHAGDSFAQLLSEIPDIERVDVSRESRDRDNRGNGRDRDNRRGNDRGDRNNFNRRP
                     FQERNNNFERREKSVYDMVKEETEQLQSKINSNFIETKPSIKEELIQQVSEGLQVKLD
                     VPHTKGMEAPIKPIDNLETEITKKEEIPKKEKPKEELKKKAIVLPKITDEIKENLGKI
                     IDDIKDKKEARLLNEKFRRISSCSAADLEHKLREFSSDKLFAIAVDQELNDTIKDLAK
                     ERGASILVVRNSNGIKDSQIHVLTYQELNK"
     gene            complement(4389..4760)
                     /locus_tag="PLK55_01670"
     CDS             complement(4389..4760)
                     /locus_tag="PLK55_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35677.1"
                     /translation="MNKVNLTKTVGAFITTIAILLLVFSIVDLFNRVDYIVEYDACVN
                     YSYNDTTLTACKLNASTGLGFKIRENQLRLSTNQYLMVYIASLLKILFSVTIFIIGMT
                     FYTSSSRTYIETKKVVAKKKR"
     gene            4864..4936
                     /locus_tag="PLK55_01675"
     tRNA            4864..4936
                     /locus_tag="PLK55_01675"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4897..4899,aa:Val,seq:tac)
     gene            5083..5164
                     /locus_tag="PLK55_01680"
     tRNA            5083..5164
                     /locus_tag="PLK55_01680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5117..5119,aa:Leu,seq:aag)
     gene            complement(5165..>5446)
                     /locus_tag="PLK55_01685"
     CDS             complement(5165..>5446)
                     /locus_tag="PLK55_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOZ35678.1"
                     /translation="ATSALLITLGIILMKLKKRNLFFGIRTPWTIVDDDVWIKTHKLG
                     GWLFIIIGITTLFALLYIKYFTIIFFTEIILLLFVTFIYSYLLYKKNRN"
     assembly_gap    5447..5546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5754..7514
                     /locus_tag="PLK55_01690"
     CDS             5754..7514
                     /locus_tag="PLK55_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOZ35679.1"
                     /translation="MKKLAIIFLFVVLISAVIYSENQNNILESSAMKTKYISLDVNIV
                     LPINFTNYTSSDEFTYKTAIFQNLNNQKIDVSAYYYDNDGTKILGEILTEGENKYAFF
                     KIKPITKNQYVFYISGQIVSEDKFVLENLRAELIPITEEIKEYTKSTKFIQSDSSEIR
                     TVANYLKKSNDLENLVYVTNWIHTYLTYDMDYVSMVNDSLSVLSNAKGVCDEFAILEA
                     AILRAQGYPVKYVVGYANTSQEWGPHAWLDVYIPGYGWIAVDPTYNEVGSVDATHIVI
                     EKLKDPRDSKDSVTSTNDVAVKFGDKKQYFTHQEVKTYEQLGLGNDLDIDVIMPREAY
                     QSSPHIAKLQLKNKTNNHLMVLIASQLAPDFKQIYPKARKIVYYLKPLETKKFDYYFK
                     LPELDNSYIYEFSYISQYKDKTTDLNIYHNRGDYQELFFVYEPVIYYQNGWFFFENTI
                     LNYTKKEKNISYAFDYDGIDSSENKNISKYSEFTYQKIFKKIDNGNLSYVLSGDYNSS
                     NTTQILPTIETPYVFDVNIDQNAFHSEEDKNKSEIFEKIKEDSVFEKPKPNYLMIIFG
                     GAFIVLIIGLFVSKMIKKEQ"
     gene            7568..7639
                     /locus_tag="PLK55_01695"
     tRNA            7568..7639
                     /locus_tag="PLK55_01695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7601..7603,aa:Gly,seq:gcc)
     gene            7664..7975
                     /locus_tag="PLK55_01700"
     CDS             7664..7975
                     /locus_tag="PLK55_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P Rpr2/Rpp21/SNM1 subunit"
                     /protein_id="HOZ35680.1"
                     /translation="MKGKDEYKKRVYFLIDFAISENNLKTDNPYIKDYIGLAFDISKK
                     INFRFPKELHRKVCKFCYGIRTSKNTKVRTITENKNHKKQRYLKIHCLECDKIKKIAL
                     K"
     gene            8143..8562
                     /locus_tag="PLK55_01705"
                     /pseudo
     CDS             8143..8562
                     /locus_tag="PLK55_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
     gene            8697..9011
                     /locus_tag="PLK55_01710"
     CDS             8697..9011
                     /locus_tag="PLK55_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35681.1"
                     /translation="MAKQKSREKKEFLIAQRDKVGPGYLNTPVWIIQKAGKRIWNRRQ
                     KRNWKQTEFGDEFRKLQIKKADAVKKTARVSGKKEKSSRRKVRKTRNTVIVRNPIKKK
                     RK"
     gene            9019..9453
                     /locus_tag="PLK55_01715"
     CDS             9019..9453
                     /locus_tag="PLK55_01715"
                     /inference="COORDINATES: protein motif:HMM:NF002258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HOZ35682.1"
                     /translation="MPSENYIIPLNKVYDNKPRSRRANNAITEIFNFLKKHTRMEKKD
                     VIISKEVNEAIWSRSIQNPPRKIAISLKIDSGKAYVFLKDSKEFKDFGKAPEQKEKPK
                     LKEAVQKAVKDATSQKKMTAKTVKKMSGKTEKRAAEKTESIS"
     gene            9461..10132
                     /locus_tag="PLK55_01720"
     CDS             9461..10132
                     /locus_tag="PLK55_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HOZ35683.1"
                     /translation="MKLDRSTIYNSPFLNIYSYCTEKICLVPHSVLPKEERKIEEILD
                     VKVIKASINRSGLLGIYLSGINEKIVVEKNSIHSDEIEHLEKEGLKVKTIKDENNAFG
                     NLLAVNSNYGIASPLLLPETISELRKFLKVEIEQKNFAGLDLPGSSIFVSDSLFLINP
                     RIDIKEFNYFKKKFNVPGKAITANYGDVFVGNDITGNKNGILVGETTSNIEMTRIDEL
                     VVDLK"
     gene            10149..10631
                     /gene="pfdA"
                     /locus_tag="PLK55_01725"
     CDS             10149..10631
                     /gene="pfdA"
                     /locus_tag="PLK55_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HOZ35684.1"
                     /translation="MAKDIKENKTRKTIKLDKNQTVKLYQNKEKELQGISQRVEQIDG
                     ILQEMQKAEIALKQIKKLKSEEKILVNLGAGVLIECEVSNKSSIKVMLPGQIMTERGI
                     DEVIEDIDKRNKELHSAKEKLVKSYNQTSQMLEQISKVFREMQLREEQKKKSTDTSIE
                     "
     gene            10652..12247
                     /gene="ftsY"
                     /locus_tag="PLK55_01730"
     CDS             10652..12247
                     /gene="ftsY"
                     /locus_tag="PLK55_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOZ35685.1"
                     /translation="MFDLLKNKFKSFTDKIFGKTKEAIAREPMTIEGSQGTKKSQSDL
                     FSASETGSAKEKTEEETATIQESEPVVETKQEETISQEIQTIAKEVAESKSIKELTEE
                     AEELEKTLDEPLEEDALILGHPKVEVPKPIIEQPEIEQSILEEPKPKEHHSIFEIFKP
                     KPKPQVPIEEPRLPKTIDERPIPTPRAEIRPTPVEPIKPVIERPFPKVEQRPIPTEDS
                     RSVPQTTADLTVKKTTEIKKSFVTSIKSIFSSKVKLSEKEISEFLQEFELSLLEADVS
                     IVSAENICKDLKEKLVAASFSKENPLEDIKEHIRESLKTQLDIDCDINNYIDQTKKPD
                     SPFVIMFIGPNGAGKTTTISKFAYHYKQEGKSVVLASGDTFRAGSIDQLEKHASAIGV
                     KIVKQNYGSDPAAVAFDAVTSAKAGKIDYVFIDTAGRQENNINLMQELKKIKKVVNPN
                     LTIYIGEAQAGQAIVDQVSGFEKEIGVDGVILTKLDTDPKGGVAISILNELKKPIFYV
                     GTGQTYEDLEAFSPQFIIDRVVI"
     gene            12301..12516
                     /locus_tag="PLK55_01735"
     CDS             12301..12516
                     /locus_tag="PLK55_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35686.1"
                     /translation="MNKKLILILSIYLIVISAISLVIVSNNSSIDTTTEEKSEIVNTI
                     FLSPSRSKIQGTVFQERGGIIKAIMLK"
     gene            12591..14006
                     /locus_tag="PLK55_01740"
     CDS             12591..14006
                     /locus_tag="PLK55_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOZ35687.1"
                     /translation="MGFGDKLRDALDKIKNSMHVDKELIKEVIKEIQRALISSDVNIQ
                     LVLNISKEIEEKAFEEIPSGITRKEHIVRLCYETLLKYMGGEVKTPLPENPETILLCG
                     LFGSGKTTTAAKLSKFYKKRGLKVGLICADTYRPAAYEQLKQLSDMVEVAFYGNPKEK
                     SAQKVTKEGLDYHKKNGCNLIIVDSAGRSALDDELVKEIKEIDDVFNPKFSLLVISAD
                     IGQAVKDQATAFNNAIGVNGVIITKLDGSAKGGGALTACYITKSPVYFIGTGEKADDF
                     EIFDSNRYLSRVLGFGDLESLMEKIAEVTKEEEMEKLKDINPEDIMKGNVTFGVFKQQ
                     LEFSKKLGPFSKVMGFLGVGNKISNEQKELGEKKMAKYRAILNSLTKEELANGPDFLT
                     HSRMDRIAKGSGTKIEDIKELSSHIKKMKKMFKGMDAKKMEGMAKQFEGQDPESIDMS
                     KINMNQFANIKGMKKKKLRLK"
     gene            14014..14841
                     /locus_tag="PLK55_01745"
     CDS             14014..14841
                     /locus_tag="PLK55_01745"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOZ35688.1"
                     /translation="MFETIFVIRIILLLLAVALAAYFDYKTGYIYDWISIPLIAIGLI
                     INIFTYSFKSLIPIVLISAGIYLFGYFAYYFGKIGGGDIKLFIGIHMILPYLNNQLLI
                     FWVIILSSLLSVMFVSISYAFKLNKKIKINKVLLKKKKSKIFKSAILFFVFLAFVIFA
                     TSTGDMPKIIYITIPPMFFGSIIIIFEDEVKKYIYLKQKPVAKLEEGDVFASEFATKE
                     LITKLKLGKRTVLEKEDIKRAKLLRLKALPIFDNLPRFGVYILLGVLCVLIFGSLLL"
     gene            14883..15983
                     /gene="tgt"
                     /locus_tag="PLK55_01750"
     CDS             14883..15983
                     /gene="tgt"
                     /locus_tag="PLK55_01750"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOZ35689.1"
                     /translation="MYKITNESAGARCGEFKTSHGKIKTPFYMPVVTKGAGKFLTFNS
                     LENLNTQCFISNAYLLYLKPGLETIKKAGGYHNFINWQKPIFTDSGGFQLLDPQFLIS
                     LNDKGVKFRSPFSGEPHFVSPESLMNIQNTLSSDIAMVLDHVLGPDHTKKEFSEATTR
                     TIAWARRCLDSHKNKNQKVFGIVQGGTYKDLRKECALAINDMGFDGLAIGGLAIGESK
                     ERMFQAIDYSLEYLDKDKIKYLMGVGSPEDMIISIGKGVDCFDSIYPTKMARHGMIFT
                     RNGILKIGKSYNSHLFKPIEKDCDCYACKHHTISYLHHLYRLDEPAAKILLSEHNVRF
                     IHRLMEDARKAIEKGVFDKFQKEFLKNYLKNK"
     gene            16122..16415
                     /locus_tag="PLK55_01755"
     CDS             16122..16415
                     /locus_tag="PLK55_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35690.1"
                     /translation="MQSKTKIVRYPRLDTLMMIEKIIEDAKGDFTVRQIWKKLPKKVM
                     WQTYITALDYLESIHKIAIDKKHVIWIWGPEEIARVKRQKLEVKSLRNRNRIL"
     gene            16334..16723
                     /locus_tag="PLK55_01760"
     CDS             16334..16723
                     /locus_tag="PLK55_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35691.1"
                     /translation="MGPRRDCKSKKAKTRGKVLKEPEPYFIDPTIEKLYYELKESTRF
                     EDKELYSNLKKAITKIQENPYCGIRVPSKLWSKEYITKYDIKILYKYDLSNGWRLTYT
                     IRGQQIEVISIIIEWFCHKKYERRFKY"
     gene            16798..17328
                     /locus_tag="PLK55_01765"
     CDS             16798..17328
                     /locus_tag="PLK55_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35692.1"
                     /translation="MAKKEKTKIQKKKVKSTDNKETKKYFFRFVESNNAQIVLLILSI
                     ILVVSSFVNTIFCYLGKGGDFGSSFFLFVPSVILLLYCFKSKLVKVFSKMVNILTHIL
                     IVLYLLFGIYAFGNDIIIDVFLAIMFSFIVFYLILLIFKNQKIMKYSQLFNIALFFFI
                     LGLLAILLIQFMNINL"
     gene            complement(17323..>17727)
                     /locus_tag="PLK55_01770"
     CDS             complement(17323..>17727)
                     /locus_tag="PLK55_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOZ35693.1"
                     /translation="LREHGILKSPEKITSPRDAFEYLLPKFLDCKEEKLIAVFISPRN
                     EVISCKILTTGTSNQTLISSKDIVLWAIREEASGIIVAHNHPTGICEPSTDDKISTEQ
                     LGKAAKLMDVSLIDHLIITENGYFSFKESGLL"
     assembly_gap    17728..17827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17828..19201)
                     /locus_tag="PLK55_01775"
     CDS             complement(<17828..19201)
                     /locus_tag="PLK55_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOZ35694.1"
                     /translation="MKLKIKTIIATGEGYTVEFKEAFSDNIGKTICAFANGAGGRIIL
                     GVKDNGQVIGFRLTNKLKSQIIDIGRNIDPTIQLKVNEESKITIIEVPEGKEKPYSNC
                     GQYYLRIGANSQKMRREELRKFFQNEGLIRFDEKINSSFKLSEDLNQIVLIQFLQKAK
                     ISNILPIKQILQNLSIFDGKHLKNAGVLFFCKTTTNFFVSAKITCVLYKGNNKIEILD
                     KKEFEEDLYSNYHNAFTYICSKLNTNYILTGREREEKLEIPERAIAESLINAIAHRDY
                     FSNGHIQVDIYLNRIEIANPGGLISGITKKEFGKKSVPRNPLLFGLMAKIMLGEMAGT
                     GIERIKKAMKEYNLKCEFDISENWFTIILHKPISLILNKDLDKDLDKDLELNNNQKQI
                     IIEIQKNKNITQKELSEIINITEKNIRNNIKKLKSKAIIERVGPDNGGYWIIKKGEMN
                     NNENKRNV"
     gene            complement(19198..20013)
                     /locus_tag="PLK55_01780"
     CDS             complement(19198..20013)
                     /locus_tag="PLK55_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HOZ35695.1"
                     /translation="MNEITVLNKRVSYYSKNEGDYISLTDIAKYKNPEHPADVVKNWM
                     RSKENIVFLGLWERINNPDFKLVEFDQFLKMAGLNAFVLPPQKWIEKTGAIGIISKSG
                     HNGGTFAHKDIAFEFASWIFAEFRLYLIKEFQRLKIDESQRLKLDWDIKRNLAKINYH
                     IHTDAIKNNLIPSKISKTKENIIYATEADILNLALFGMTAKDWKEKNPKEKGNIRDYA
                     DVSQLVCLSNLENLNAVFIKEGLNKLERLEKLNNIAIEQMTILTKTNNPRKLL"
     gene            20250..21194
                     /locus_tag="PLK55_01785"
     CDS             20250..21194
                     /locus_tag="PLK55_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOZ35696.1"
                     /translation="MITNKKAITPIVASILLIVVAVILVTIVLNWGRSFSNSSLATAD
                     TEKLSKSDASEFVYTKAFSDGVIQFNYAPPDSLTSQNIIITGYKIFSDNNETAEIVLD
                     SNHTLRQGLNVIPLTDFSDQNITSRKMNIQLHTSDGKYITLNNVTNNNPYVILELPYI
                     MNGETKLYIHPTDNSTGNITWGCSGTLIGAGAQSATDGATNTAAIIAGCATSPIAASV
                     CENLEAYGYDNWYLPAKDQLAAIWNACTEVTKSNACMNAAINKGDYTEEWSNIVNDLY
                     WSSTETWAGGAYGIDMDGGHFGSPNKTDTSYVRCVRDQ"
     gene            21416..22630
                     /locus_tag="PLK55_01790"
     CDS             21416..22630
                     /locus_tag="PLK55_01790"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOZ35697.1"
                     /translation="MRYSHSRIETYKSCPKKFYFQYIEKPDIEEKKGIEAFLGSMVHL
                     ALEKLYKDLKFTKLNSLEEILEYYSEEWEKNFDLTIEIVRLDYTAEHYKEMGKRFLTD
                     YYEKYHPFDSGKTLGLEMEITLRFIDDYGTPFDLIGYIDRLTMINEEHFEIHDYKTNN
                     SLKTQEEVDKDKQLALYTIAIKKMYPTVKRVDLVWHFLAFNIELRSSRSDKDLEELEK
                     EVVKAIQEIERKQITNDFPTKESALCDWCAFKEICPVKSHSFKLSKLPVNEYLNDDGV
                     KLVEEYLKLTKEKKEVLDKLDPELEKLKEALVAYSKTNNSERVYGPEGNSILVKEYEN
                     YSLPEKDSRQRQDFEKLLKESGTWEMIAEVNSFNFSKAVTSGLFSKDFLKRLEPLISK
                     GKTTRLYPSSKK"
     gene            22691..23800
                     /locus_tag="PLK55_01795"
     CDS             22691..23800
                     /locus_tag="PLK55_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOZ35698.1"
                     /translation="MSEKDQIKIHSIKVDSSENKNIKEAPKKTKLIHLSVSGKSEKDI
                     TQVKKEVKKKLTFKDFVFNLSKLINSIPNFFANFFDTSFRTIAYTIVGTMLSFGAALV
                     LFHENAGLFSVFFLTIFLAPIAIKETNQNVLLAGRTQKVEEKGVSLTKLKVKESKKFY
                     LKDLFEENKKTLYIYLFFFLGIMLVVISLIAIMPVDFSAQLFSEQGWNTALMPTRSIG
                     FAGLEKTSIFFDILKNNFSVMLVCFIIALIFPLGALLMIVWNAMYWAVSFSQYALFYS
                     KVYSVKLLSILFPLLLSVSLHTIIEAICYFFAVISGTMLSMSIKSEKLESDRFFYLFK
                     YCIILLAFSVFFLILGSFVEVFLFDGIKNFFFSLF"
     gene            23884..24975
                     /locus_tag="PLK55_01800"
     CDS             23884..24975
                     /locus_tag="PLK55_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35699.1"
                     /translation="MTTNDNKEESIMSPHHSKKRILIWQLISAVLFIALIIAILLAVT
                     NKPETTNSPMTGNIGLVNPTTETKINQKVVDDTVSFLKEAFQLPEVTVKETKVVDGLY
                     EITITIEDQDMPIYTTATGEHIIVPGMGLVNKQEVLVQMEEAKALEEAQAEAEANIAK
                     SDKPKVEVFVMSHCPYGTQMEKGLIPVMEALKDKADIQIKFVDYAMHGETELKEQLNQ
                     YCINKEEPEKYLPYLKCFLEAGKTTECLASTNINTTKVNACISATDTEYKVTELFNDE
                     STWSGGAYPQFNIYKAENELYGVQGSPSTVINGVLVSNMPRDSKGILDKVCSVFNTAP
                     EECNTALSSETPSAGFGWGDSGAPTDATC"
     gene            25078..25953
                     /locus_tag="PLK55_01805"
     CDS             25078..25953
                     /locus_tag="PLK55_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ35700.1"
                     /translation="MVSVIEKKNGTKKAYYLHMTLRSKTGYKNLDKYLGIKLPADLRT
                     KKLDFFIDSQQGQLEKISEQISKSNSQKLKMPKTVQENQLFDFAVKFTYNTNRIEGNT
                     LSLKDSYLLLRDQISPQKSMRDIKETESHQKLFLELIKKSQNITYQNLVNWHYFLFKD
                     TKKDIAGKIRNYQVGISGCKFIPPSPAELSAELSGFFNWYNKNKEKYNPVVLAGLVHL
                     RLVTIHPFGDGNGRITRLLMNIILSNNNYPLFIIDYKHRKSYYSALEKSQTKDDDSYF
                     LSWFIKSYLIFIKSK"
     gene            26008..26664
                     /locus_tag="PLK55_01810"
     CDS             26008..26664
                     /locus_tag="PLK55_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOZ35701.1"
                     /translation="MKITLYNKLKKQQHRDIASLQDELVKIIYGIFPEAVFHGGTSIW
                     RCYDGNRFSEDIDLYFYEKQDIEKQLKEELQGKGLNLIKFKKTENVIFAKITDKNIEV
                     RLEIRLLEKSNSIFKTKSIKQYQNLDGSSITVFCLSPEELLLEKALAYKNRKLIRDIY
                     DVYFLQSLANLSNEQKKKLKEIVDSWEKPEDEKNLKAIVYSGAIPSFAQLLSEIKRRY
                     "
     gene            26664..27242
                     /locus_tag="PLK55_01815"
     CDS             26664..27242
                     /locus_tag="PLK55_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35702.1"
                     /translation="MTYENRLRDVYKEEYKFTKKDASKLFSKDKISNEYLDQLLHNLV
                     KQNKIIRITNGSYTFKKDLMVSGFAYSPFYYGLQEALSLRNLHEQETNPIIITTNKIK
                     PGIKTIQGSNVLLRRIDCKYFFGFDFIKYYDMEIPVSDYEKTLIDFVYYNEPLSKEVI
                     NELKKKIDKTKLQNYLLRYSKKIRAKANLLLK"
     gene            complement(27244..27807)
                     /locus_tag="PLK55_01820"
     CDS             complement(27244..27807)
                     /locus_tag="PLK55_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ35703.1"
                     /translation="MIPNRTQAMVLLKEHLKEKPNIDHSFLVAYGLLGISKLLNLSET
                     EQDYWFVIGLLHDLDLESFNEDIKEHTLVTEKILLEKNVDKELIDDIKSHNEIIGVER
                     NSNLRNALYSLDGLTGIIRAYVLMRPDKDITKAETKSIIKKLKDKYFAAAVNREQIYL
                     CEKTLGIEIEKFVSAALEEIKKNYILE"
     gene            complement(27814..32976)
                     /locus_tag="PLK55_01825"
     CDS             complement(27814..32976)
                     /locus_tag="PLK55_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ35704.1"
                     /translation="MNSKGANVIFTSIMIILLSVAAMIIIIGLVVPFFTSLREEINFN
                     HNKENLILINQELLDLKTNDINTFKNLNLDVVDEIVFDATTNTVYIKQKISNNSFKNF
                     KDANYGNLAIVKANGLMTFTLYLDGIFTLDKTIVINGNYTLRLTISDIINGIPVISIT
                     QYLGQVYLTISPKFNTFIGSLEIILTATPTDANIYYTTDSSEPTQESTLYTEPFTITD
                     STTIKARGYADGYIPSAIISKQFIGLELLDFNFFNILPDGNAFTKELIVTANIEPIDA
                     NIYYTLDGNDPTENSIPFVGPITITDDNTILKLRAYKYAYNPSEIITKIFTEIMGVLF
                     YNKYDTNTTTDLSDNNLSVIESKVLFDENGANFTNDSNNYIYIENDWNNSNPFFNLGV
                     DNFTLEAFVTDNYKGHIYSQRGFVGGINLFIDENSYLNFKLSGSDQLWGVYGVADDDE
                     YIYLSITGTQSIQKRRKDNWKLIATVGGPRLGNAIDSFSAPYALAVDDNFLYVSDYSN
                     HRIKKLNKSDLSYHSMLSNGPGSASNQLNGPLGIAVDENYIYVIDATYHRVKKLNKET
                     FQLVATLGGTSSGIGDLQFYNPKGIAVDENYIYVTEDYYHRIKKYNKTDLSFVSKACG
                     TSYGTGTDQMRAPKGLTVDDNYVYIAEYTNNRIMIRNKNDLTYVALISSTGGIGNDNY
                     HYNPTDIADDENYLYLLTATDNKIKKIKKSDYSVYEIFSDTDPLPENVFVPSGLTNDG
                     EHIYYTDKALHRVVKRRLSDLAVVGIIGGPNPGSTSTTFYYPEDLAVDDEFLYVTNRQ
                     YHQVKKYNKSDLSWVYTYSPGSGTLGYPYGIAVDGNYLYVTTGYGSTLHTLKKIDISS
                     TPWSVVANIGGATGSTDDTFSNPYSVTVDDTYVYVADYTNQRIKKHLKSDLSFVSQLG
                     LTGYSNYLPNTLYNPAYVIYDNNYLYISNNGYARILKYDTNFNYISNIGYSTGYAYSE
                     FLSTGGLTIDNGYLYVADPTTGIIIKRNLSEFDVNISFVGSGIDFNFFYSPYSVDSDE
                     NYYYVIDSIIHRLQKVDKTTNQIVSYRGGPYGVASGTTQAIRSNFFNYPNYILVDGDY
                     IYVSDRTSPRVIKFNKSDLSYISQFGTGTNGATTTTLNGPRQLAIYDTNLYVADYGNS
                     RIVILNKDTMEYITHFGTNGTGNDQFSGIFGVGVDINYIYTVEYTNNRIKKIDKNTFA
                     YVTRLGGTGSGSGNDQFSSPTYLSLDENNIYVVDYANNRIKKHLKSDLSYVTKFGSSG
                     TDNNYFYLPIDIKYKDENTLLIADTGNDRLKIHHPSDLSYITNINLSTYNNDGVYNPW
                     GVAVDENYLYVSDTGNHRIIKKNKSDLSTLYSVGGPNSGSGNDQFNSPYGIAIDQNYI
                     YVSDNANCRIVKRNKTDLSYVSKLSNGCGSSNEMVQYIFGIAVDDNYIYVVERSWHRI
                     KKFTKDTFTFVASVGGTVSGSGDDQFNNPTDITIDDNYIYVTDLSNYRIHKRNKNDLS
                     LVKMYGLGVNASSTTDSFYEPTGITIYNDTLYVSDSRNYRIKVYDTNLNFIYKFGRLN
                     WEKDGLYLPQGLALDDNFIYIADSSNSRIVKRAMSDLENIKNQEKSGYFGIQGYNGKT
                     LTSIGTVTGDSQIAIVRIDDNFCMYINGALDNCQSFYLHPTNLTFPEINFRFNSSATI
                     TTYLLPENQNLYPHIIGNAGLPMPGNGHIKYLRVFDTNLSDSDINALYQSYK"
     gene            complement(32973..33575)
                     /locus_tag="PLK55_01830"
     CDS             complement(32973..33575)
                     /locus_tag="PLK55_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35705.1"
                     /translation="MNARFLSSRKGNRRVFLSLGKEFNKEVIKMKIKTQKGMSAVIST
                     LLYILIAIIILGVVTYSAMELLDGVKEQSNYDTMLESMTTLKIAVEDVVDDKKDIELV
                     VKNPGEIIIDCTNNLITGEIDYFGKYKDEIVMINEIATYKNNNTIYFEYNLDNYKRLI
                     LDCNDFIISNKKTTLNIKYQEYDDENDQILINLECMECRT"
     gene            complement(33572..36571)
                     /locus_tag="PLK55_01835"
     CDS             complement(33572..36571)
                     /locus_tag="PLK55_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOZ35706.1"
                     /translation="MIKSKKAVNEIISTILIMLVMVIVVGIIITGVNSQISKGKEKTN
                     YDNSIKVRDMVFSEIMEIYNSPIEATKEINFSLSNLELTIDSDTETIEICSVGKNLSF
                     FADGKRLEQDNNKYVYRELQKICSGVAFENIDLIRSITLSNKEQTRLVFKKITENKIS
                     VSVDNETSTSWYTQKNEYVYDENAGEWLYRKKILINSGNLVGDLNNLSVQLDLEDEDL
                     MSYANIDASDIIFTLGDGITKIKREIVDYNSDTGALSVLIKGPQFNYGPDINNPVYDD
                     NTYRKKITIDHTKVDGDLNDFPMLISIVDENLANLGANADGNDIYFTASDGTTRLKRE
                     IESFSKIYRKKITIDHNLVDSDLNDFPMLISIVDENLVSYGANADGNDIYFTTNDEIT
                     RLRREIEDYNSETGALVAWVKIPELSSTTDTDIYMYFGDSEENNTNDTDVWDSNFVGV
                     WHKNDLTTTTIADSTSNSNNGTKKATLEPSEIIGKIGYAQNYDGSNDYINTGNKSAYN
                     SQIITIELWGKLNGAGSHTDGTYLINKGRASSVPYFSYIIRHIEDTNQFNLLLGFSDN
                     TYGSIYANEYPITDNDWHHFVGTYDGDDLNFYINGNLIQSRNYINKQIKFDFPNNELY
                     IGAWNTPVKRAFNGNIDEVRLSNTARTTSWIKTEYNNQSAPETFSAVGELEKTAVLFA
                     RIEIPELSSTTDTEIYLNFGDSEENNLNDINYEDLSLWFNAKNRNSYPGSGNTITDLA
                     GDTSWTFNQSLISFDNVDKSFNFPNNTTAKNIITQDNVTLESDTNSLTYYFWAKVDSP
                     NSASYNSLLNDRTQGTTPFIWIFKGGNSLYMQNTDGVSNFINITSSNYFLNYTNQYLN
                     IVATVKYDENASQVKYYRNGSLINTVNTNKLLLPNQNNTKKIGIYSTAGTHVWVGDIK
                     EFRIYNDILSGEFIQTEYNNLNSPETFYSVGTNEIVENIAGYDFTKSTTDTPIYIYFG
                     NPDANISDNSIEDSNTATISIGVLEKS"
     gene            36673..36744
                     /locus_tag="PLK55_01840"
     tRNA            36673..36744
                     /locus_tag="PLK55_01840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36706..36708,aa:His,seq:gtg)
BASE COUNT        13331 a         5404 c         5787 g        12023 t
ORIGIN      
        1 taatatgtat tatttattaa ttaataaata aatgatatat atgaataaga aaaaaccaac
       61 aaaaataaca aaagaaaagt tttccccaac tcttaatacc atcttgatgg ttgaaaagac
      121 attgcaagaa atgccagata gtgttgtcaa ggtttcagaa ttgaaaagat tgttaccaaa
      181 gcaaattaat cacaacaccc taaagaatat cttagagtat cttcaagaaa gtaacaagat
      241 atttattggg gttaaaggaa ttgtgtggat acacaacgat agcccagtat taaggaaagc
      301 aattgcagat gcagaaagaa acggtagaac actatgacaa aaaaagtaac tgtgctttat
      361 tctaagccag ttgaagaaac cttaactttt ctagaacaaa caaaagaaaa taataaaatt
      421 agtaaatcaa tatacaactc atttcttaga aaaatagaat taattaaaag caatccaggc
      481 tatggagata atatcccaaa gccacagata ccaaaagaat acattgagaa atataaaata
      541 aacaatctat ttagattaga gcttgcgaat tattggaggt gtctttactc tttgactgat
      601 aaaaaagaaa atacagtaga aatcatcgca tttgtaattg acttattaga tcacaacaaa
      661 tacaataaga aatttaaata taaataacac taattcttgg acaaccgcgt agccaaacct
      721 ccgaaggata tatatatata tcggtttttc atttatttat aaaattaata attaaaaatg
      781 gagatgatta agaaatgccc ggagcaccaa agccaaaagt aaaatatgaa aaaccaacaa
      841 ttaaaaaaag aatcgcaaca gccgcagctg tgggtttact agggggatca atagctttag
      901 gattgcatgc aatagaacca ttaagagcta gttcacaaag atctgatgtt aagactgcga
      961 ttgcgcaaat agataaaaaa aaggcagatg tcggatcaag accggtagaa tattattcca
     1021 agaatccaaa tttgataaga gaaacagtta attcttataa tcaaaaagca aagctcggac
     1081 aaattttctt ctatgatggg aaaaaaatta aatcagttta ttttgatcaa acaagattaa
     1141 atcaaatcac aagcattatt ccaaagagcg agaaagcaaa gattgatagt ttaatttcta
     1201 gaaaaataga aaaagtagaa gcaactgcaa aacaaaatgt tgttttagga aaaaaaataa
     1261 ttgatagagt tggagaatta gattatacaa aatatttatt atcattaact ccaagagaac
     1321 taaaaaatgc atttcctgaa gccgaaattg cagtaagaaa agcacttgaa agaagtaatc
     1381 cagaaatttt taaaaaaata aacgcagaaa aaaaaggagg ggttttgttt agtatgattg
     1441 taaatacaat ttggggatca ttatggggtg gtgctttttt agcaaaagga aaaaagataa
     1501 aataaataac ttatttatta tttatttttc taacctttaa ttcatttaat gcttttatgg
     1561 cactgtcgta aaggcattta tttcttaaac tcttttcttt ttttgcttct ctgtcaagag
     1621 aatttattat gtcttcaagt tcaagatatg ccattttttt tcacaattag aatatatatt
     1681 attaatatat atttatgata gaaagagtat ttaaacctgt ctagagggct ctaaaaatcg
     1741 ctaataaagt catcaaatcc ctttccttta gcctgtatct cttctttttt gatggcatag
     1801 gcctttttgg tcttgtttag ctggtctacc cttataaaaa accaagcatc tttaccaagt
     1861 ctccaggcaa cataggcttt taggccacta agcttctccc aaagctttag ttcctcgacc
     1921 tgctcgtctc taatgttaag atactcgcct ttcttggtct tacactctat ggcaagagcc
     1981 tttccgttct taaagccaag aacatcagga gttggaagct gagaggagcc agaaccagca
     2041 accctagaca ccgcaaagcc cttattaaat agcataaata ataattctct ttcagcattt
     2101 gagccttttt gcttgttttt cattattttg tcacatctat agatataata aaagtagcct
     2161 ttatatactt ttttgtctat taatatatta taagtttaag caataaattt ataattatat
     2221 tcaaaaatta aaagattagt aatttagaaa ttaagaagat taacttttat aaggtatttg
     2281 aaatagatgt ttgaaaaaaa ccaggtattt tcattgcaag ttatttgcaa tagcgacagg
     2341 caagaaatcc aaaagattga tgataatact tataaactaa aaataaaaag cattgcacag
     2401 aaaggcaaag caaacaaaga aatctttgag tttttcaaag ataaagggtt aaagattgag
     2461 ataataaaag gcgaaaaatc aaacaaaaaa atgcttaaaa tcctttaaaa gtgtcttctt
     2521 tttctaatgt ctaatctatt tttgaatgtc aaaacaataa aaggagtgac atttaacaat
     2581 gggaaaaaca tatattgaaa caatcaagta catgattgtt gctgattttg aagtaaacgg
     2641 tcttgttgaa aggccagatg tgattggtgc tatttttgga caatcagaag ggcttttagg
     2701 agcgaatcta gacattaaag aactacaaca gaatggtaag attggcagaa ttgaaataga
     2761 atatacacag aaccaaaata aagtaagagg agtacttaaa cttccatgta gtgtttctat
     2821 ggttgaaact gcaatcattg ctgcatccat tgagactata gagaaagttg gtccctgtga
     2881 tgctgttttt aaggttaaac aaatagaaga cacaagagga ctaaagagac aacaaataaa
     2941 agaaagggca attgagctat taggaagact aatgcaagat gagattccag acactaaaga
     3001 gttaatggac gagataatgg atacttttaa aacaaaagac ataatctctt atggttcaga
     3061 aaaactagca gctggtccaa caattgattc aagtaaagaa ttaatagttg ttgaaggcag
     3121 agcagatgtt ttaaatcttt taagatatgg ttttactaat gttgttgcaa ctggcggagc
     3181 aagaatacca agatctttag aagaaatttc tagacaaaag tcagtaacta ttttcttaga
     3241 tggagaccac ggcgcagata ttcaacaaca gcaattatta aaatcaataa aagtagatta
     3301 tattgcaaga gcgccagatg gtaaagaagt tgaagagcta acacaaaaag aaattaacca
     3361 agcattaaga agaagaataa aatcaaatta tgtttcttca agtaacaatc aattccaatc
     3421 acaacaacaa ttcaatccaa atcttacaaa gcaagcgggt attgttaaag caacaaagtt
     3481 ttcaacaaca aatgatcata agattgcaaa tccaaagttc tttgaaaata aaccaaataa
     3541 gtatttacag actcaacaac caactaatag accaatgcat gatcaaaatc acgcagggga
     3601 ttcatttgct cagctcttgt cagagatacc agatattgaa agagtagatg tttcaagaga
     3661 atcaagagac agagacaatc gcgggaatgg aagagacaga gataatagaa gaggaaatga
     3721 cagaggagat agaaacaact tcaatagaag accatttcaa gaaagaaaca ataactttga
     3781 gagaagagaa aaatcagttt atgatatggt aaaagaagag acagaacaat tacaatcaaa
     3841 gattaattct aattttatag aaacaaaacc atcaataaaa gaagaattga ttcaacaggt
     3901 ttctgaaggt ctacaagtca agttagatgt gcctcataca aaaggtatgg aagcgccaat
     3961 taagccaatt gataacctag aaaccgaaat aacaaaaaaa gaagaaatac caaagaaaga
     4021 aaaacctaaa gaagagctaa agaaaaaagc aattgttctc ccaaagatta cagatgagat
     4081 aaaagaaaat ctgggtaaaa taattgacga cattaaggac aagaaagaag ctagacttct
     4141 aaatgaaaag tttagaagaa taagtagctg tagtgcagca gatcttgagc acaagttaag
     4201 agaattttct tcagataagc tgtttgctat agcagtagat caagaactta atgatactat
     4261 caaagaccta gcaaaagaaa gaggcgcaag cattcttgtg gttagaaatt ctaatggtat
     4321 aaaagatagc caaatacatg ttctaactta tcaagaacta aataaataaa tccaaaaaga
     4381 aaagaaattt atcttttctt ttttgcaact acttttttag tttctatgta tgttcttgat
     4441 gaactagtat agaatgtcat tccaattata aagattgtaa ctgagaacaa tattttcaat
     4501 agtgatgcaa tataaaccat caagtattga tttgtggaca atcttaattg attttctcta
     4561 attttaaatc ctaggccagt agatgcattt aatttacatg ctgttaatgt tgtatcattg
     4621 taagaataat tcacacaggc atcatattct actatgtaat ctactctgtt gaataaatcc
     4681 acaattgaaa aaacaagtaa caatatagca atagttgtaa tgaatgcacc aactgttttt
     4741 gttaaattaa ctttattcat attttccccc ttttttaagt aaatataata ttatatttat
     4801 agccttatgt atttaaatat tttttccttt ttataatata tataaaaatc tagtttttta
     4861 tatggatctg tggtctagtg gttatgatat cacccttaca aggtgaggat cggcggtccg
     4921 agtccgccca gatccacttt cagttttgta ttaggctttt cgtcaattgt gcaccattgc
     4981 ggataactcg cgaaatccgt tgggtgtgaa cttcaaaaaa agtcaaagac tttttaggat
     5041 cctaaaaaat cagagatttt taatgagacc gaaaccagtt ccgcaggagt acccgagtgg
     5101 tcaaaggggc acggctaaga accgtgtgct tagtgcttcg caggttcgaa tcctgtctcc
     5161 tgcattaatt tctatttttt ttgtaaagca aataagaata aataaacgta acaaatagta
     5221 ataaaataat ttctgtaaaa aatattatgg taaaatattt gatataaagc aaagcaaata
     5281 atgttgtaat tccaataatt ataaacaacc aaccgccaag cttatgtgtt tttatccaaa
     5341 catcatcatc aactattgtc caaggcgttc ttataccaaa gaaaaggttt ctttttttta
     5401 gtttcattaa tattattcca agagtaatta acagtgcaga ggttgcnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnttcc agtgtgttgc tattgtttcg ggcatatggg
     5581 aatataagaa gaaagcaata atactatata ataatattat tattaaaata ataatttcga
     5641 ttttttttaa tttagtcata ttttcaccag tggcatatca caaataaaag ataattagta
     5701 ttataaattg ttgtttcttt tatttttatt gtaaaattaa aaaagaaata actatgaaaa
     5761 agcttgcgat tatatttttg ttcgtagtct taatatctgc ggttatatat tcagaaaatc
     5821 aaaataatat tttagaatct tctgccatga aaaccaaata tatttcttta gatgtaaata
     5881 tagttttacc aattaatttt acaaattata caagcagtga tgagtttact tataagactg
     5941 caatttttca aaatttaaat aatcaaaaaa tagacgtttc tgcgtattat tatgacaatg
     6001 atggcacaaa gatattagga gaaatattaa cagaaggcga aaacaaatat gcttttttca
     6061 aaattaagcc aattactaaa aatcaatacg ttttttatat ctctggccaa atagtttcag
     6121 aagataaatt tgttttagaa aatttaagag cagagctaat accaattact gaagaaatta
     6181 aagaatatac aaaatcaact aaatttatac aatcagatag tagcgagatt agaacagtag
     6241 caaactatct aaagaaaagc aatgatttag agaatttagt gtatgtaaca aattggattc
     6301 acacatattt aacttatgat atggattatg tttctatggt taatgattct ttatcagtct
     6361 tatccaatgc aaagggagtt tgcgacgagt ttgcgatcct tgaggcagca atattaagag
     6421 ctcaaggtta tccggtaaaa tatgttgtag gatatgcaaa caccagtcaa gaatggggcc
     6481 cacacgcctg gttagatgta tatataccgg gatatggctg gattgctgta gaccctacat
     6541 ataacgaagt aggaagtgtt gatgcaactc acatagttat tgaaaaatta aaagacccca
     6601 gagattcaaa agacagcgtg acttctacaa atgatgtagc tgtaaaattt ggagataaaa
     6661 aacaatattt tactcatcag gaagtaaaaa cctatgagca attaggatta ggtaatgact
     6721 tggacataga cgttataatg ccaagagaag catatcagag tagtccgcat attgctaaat
     6781 tacaattaaa aaacaaaaca aataatcact taatggtctt aattgcaagt caattagctc
     6841 cagattttaa acaaatttat cctaaagcac gaaaaatagt ttattatctt aaaccactag
     6901 agacaaagaa atttgattat tattttaaat taccagaatt agataattct tatatttatg
     6961 agtttagtta tatttcacaa tataaagata aaaccactga cttaaatatc tatcataacc
     7021 gtggtgatta tcaagaactt ttctttgtat atgaacccgt aatatattat caaaatggat
     7081 ggttcttttt tgaaaataca attttgaatt atacaaaaaa agaaaagaac atctcctatg
     7141 catttgatta tgacggaata gattcttctg agaataaaaa catttcaaag tattctgaat
     7201 ttacatatca gaaaattttt aaaaaaatag ataatggaaa tttaagttat gttttgtcag
     7261 gagattataa ttcttctaac acaactcaga tattaccaac catagaaaca ccatatgttt
     7321 ttgatgttaa tattgatcaa aatgcatttc attcagaaga agacaaaaac aaatcagaaa
     7381 tttttgaaaa aataaaagag gattcagttt ttgaaaagcc aaaaccaaat tatttaatga
     7441 taatttttgg gggtgccttt atagtgttga ttatcggatt atttgtttct aaaatgatta
     7501 agaaagaaca ataaaaacaa tagtttataa acatttttta tgttaattat attataaata
     7561 aaataaaacg cctgtagtct agtggttagg atgggagctt gccaagcttt cggcccgggt
     7621 ccgaaccccg gcaggcgtac ttctattgtt tttttgaaaa attatgaaag gtaaagacga
     7681 atacaagaaa agagtttatt ttcttattga ttttgctatt tctgaaaaca atttaaaaac
     7741 cgacaatcct tatattaaag attatattgg actggctttt gacatttcta aaaagattaa
     7801 ttttagattc ccaaaagagc ttcatagaaa agtgtgtaaa ttttgttatg gaataagaac
     7861 aagcaagaac acaaaggtac gcacaataac agaaaacaaa aatcacaaaa agcaaagata
     7921 tctgaagatt cattgtttag agtgcgataa aataaagaaa atagcactca agtgaaaaca
     7981 aaacctattt aaacaaaact attctataaa tatattataa tacttgtttt ttggcaaagc
     8041 attggttttg ccaagaaatt cagcagaaat gcgaaatatt atcttaagtt ttccaaaaag
     8101 gaaacaaaga aaaagaaatt taaaaaacta gaggtgaata atttggctaa tgtaaaagac
     8161 gtccctgctc aagttatgat aactgagata gcaaaggact taaagacaaa caaaaaagta
     8221 aagatgccag agtttgcaga atttgtaaag actggttgta gcagagagag agctccacaa
     8281 gatgctgatt ggtggtatgc tagaatggct tctattctaa gaaaagtagc aattaatgat
     8341 aagacaacag ttaattctct aagaacctat tatggtggaa aaaagaatag aggagtaaag
     8401 cctgaagagt ttagaaaagc aggtggtaaa ataattagag tttgtttaca agacttagaa
     8461 aaattaggac ttgtaagtaa atccgaagaa aagaaaggaa gaataataac tcctgctggt
     8521 caaaagtatt tagacaagat ggctgataca atattaaagg actataacaa atacaagatt
     8581 gttaagaaag aaaaagaaat agtaattaca aaagacacaa aagagaaatc tgtgtttgaa
     8641 ggttcagaaa agaaagggaa gaaagataat aagaagaaac aggaggttga agaataatgg
     8701 caaaacaaaa atcaagagaa aagaaagaat ttctaattgc tcagagagat aaagttggtc
     8761 caggttactt aaatactcct gtttggatta ttcaaaaagc aggaaaaaga atttggaaca
     8821 gaagacagaa aagaaactgg aagcaaacag aattcggaga cgagtttaga aaattacaaa
     8881 taaagaaagc agatgctgta aaaaagactg ctagagtgag cggaaagaaa gaaaaaagct
     8941 caagaagaaa agtaagaaag acaagaaaca cagtgattgt aagaaatcca ataaagaaaa
     9001 agaggaagtg attgatatat gccaagtgaa aattatataa tacccttaaa caaggtttat
     9061 gataacaaac caagaagcag aagagcaaat aatgcaatta ctgaaatctt taactttctt
     9121 aagaaacaca caagaatgga aaagaaggat gtaattattt caaaagaagt taacgaagca
     9181 atctggagta gaagtattca aaaccctcca agaaagatag caataagctt aaagatagat
     9241 tctggtaagg cttatgtctt cttgaaagat tcaaaagaat ttaaagattt tggaaaagct
     9301 ccagaacaga aggaaaagcc aaaactaaaa gaagcagtac aaaaagcagt taaagatgca
     9361 acatctcaaa agaaaatgac agcaaagact gtaaaaaaga tgagcggaaa aactgaaaag
     9421 agagccgcag agaaaacgga gtcaataagc tgaaagtaat atgaaactag atagatcaac
     9481 aatttacaat tcaccgtttt taaatatata ttcctactgt acagaaaaga tttgcttagt
     9541 accacacagt gttcttccaa aagaagaaag aaagattgag gagattcttg atgttaaagt
     9601 aatcaaggct tcgattaaca gatctggtct tttaggaata tatctttcag gaattaatga
     9661 aaagatagtt gttgagaaaa acagtattca ttcagacgag attgaacacc ttgaaaaaga
     9721 agggttaaag gttaagacaa tcaaagatga gaataatgcc tttggtaatc ttttagcagt
     9781 taattccaac tatggaattg caagccctct tcttcttcca gaaaccattt cagaactaag
     9841 aaaattcctt aaagttgaga ttgaacagaa gaactttgct gggcttgatc ttccaggaag
     9901 ttcaattttt gtatctgatt ctttgttttt gattaatcct agaattgata ttaaggaatt
     9961 taattatttc aagaaaaaat ttaatgttcc cggaaaagca ataactgcaa attatggaga
    10021 cgtgtttgtg ggaaacgaca tcacaggaaa taaaaatggg atattagttg gagaaacaac
    10081 aagtaatatt gaaatgacaa gaatagatga gcttgttgtg gatttaaaat aaaacgaggc
    10141 gataaattat ggcaaaggat ataaaagaaa ataaaaccag aaaaacaata aagcttgata
    10201 agaaccagac agtcaaatta tatcaaaaca aagaaaaaga attacaaggc atatctcaaa
    10261 gagttgaaca aattgacggc atattacaag aaatgcagaa agcagaaatt gcattaaagc
    10321 aaataaagaa attaaaaagc gaagaaaaga ttttggttaa tcttggtgct ggtgttttaa
    10381 ttgaatgtga ggtttctaac aaatcctcaa ttaaagttat gcttccgggt caaattatga
    10441 cagaaagagg cattgatgaa gtaattgaag atatagataa gagaaataaa gaattacatt
    10501 ccgcaaaaga aaagctagta aaatcataca atcaaacatc acaaatgctt gagcagatat
    10561 ctaaagtctt tagagagatg caattaagag aagagcaaaa gaagaaatca acagacacat
    10621 caattgaata aataaaaatg gtgtttttga gttgtttgat ttattaaaaa acaaatttaa
    10681 gagctttact gataaaatct ttggtaagac taaagaagcc attgcgcgcg agcccatgac
    10741 aatcgaaggt tctcagggca ccaagaaatc gcaaagcgat ttgtttagtg ctagcgaaac
    10801 aggctctgct aaggaaaaaa cagaagaaga aaccgcaaca attcaggaat cagagccggt
    10861 tgttgaaact aaacaagaag aaacaatttc tcaagaaata cagacaatcg ctaaagaagt
    10921 tgcagaatct aaatctatta aagaattaac cgaagaagca gaagagttag agaaaacttt
    10981 agatgagcct ctagaagaag acgcactaat tcttggacac ccaaaagtag aagtcccaaa
    11041 gcctattatc gaacaaccag aaatcgaaca gtcaattcta gaagaaccaa aaccaaaaga
    11101 acaccattct atttttgaaa tctttaaacc taagccaaag cctcaagtac caattgaaga
    11161 accgcgctta ccaaaaacaa ttgatgagcg tccaattcca actccaagag cagaaattcg
    11221 accaacacca gttgaaccga ttaaaccagt aattgaaagg ccatttccaa aagttgaaca
    11281 aagaccaata ccaacagaag attctagatc agtccctcaa acaacagcgg acttaaccgt
    11341 taagaaaacc acagaaataa aaaagtcttt tgtaacttct attaaaagta tattctcaag
    11401 caaagttaaa ttatcagaaa aagaaatttc tgaattctta caagaatttg agctctcact
    11461 tcttgaagca gatgtttcta ttgtttctgc agaaaacatt tgtaaagatc taaaagaaaa
    11521 actagttgct gcaagctttt caaaagagaa tccacttgag gacataaaag aacatataag
    11581 agagtctcta aaaacacagc ttgacattga ttgtgatatt aataattata ttgaccaaac
    11641 aaaaaaacca gatagtccgt ttgtaataat gtttattggc ccaaatggcg caggaaagac
    11701 aaccacaatt tctaagtttg cttatcacta caaacaagaa ggtaaatcag tagtgctggc
    11761 atcaggcgac acctttagag caggaagtat tgatcaactt gaaaagcacg caagcgcaat
    11821 tggcgtaaaa attgttaagc aaaactatgg ttcagatcct gcagctgttg cttttgatgc
    11881 agttacttct gcaaaagccg gaaagattga ttatgttttt attgacacag caggaagaca
    11941 agaaaacaat atcaacttga tgcaagagtt aaagaaaatc aaaaaagtag taaacccaaa
    12001 ccttacaatc tacattggag aagcacaggc aggccaagca attgttgatc aagtaagtgg
    12061 ctttgaaaaa gagattggag ttgatggcgt aattctaaca aagcttgata ccgatcctaa
    12121 aggcggcgta gcaatttcta ttctaaatga actaaaaaaa ccaatcttct atgttgggac
    12181 aggccagacc tatgaggatc tagaggcttt ctctccgcaa ttcataattg atagagttgt
    12241 gatttagata tccttaaata cagtaataac cataagatat ataataaaat acgagaaaat
    12301 atgaataaaa aattaatttt aatactttca atttatttga tagttatttc agctataagt
    12361 ttagttatag tttctaataa ttcttctatt gatacaacta cagaagaaaa atcagaaata
    12421 gtaaatacta tctttttatc tcccagtcgt tcaaaaattc aaggaacagt ttttcaagaa
    12481 cgcggcggaa taattaaagc aatcatgctt aagtaatttc tttttcctta atatttatat
    12541 tctatttgtt tctttaatta tatactaaaa tatattttaa ttgattaact atgggttttg
    12601 gagataaact aagagatgcg ctggataaaa ttaaaaattc aatgcatgtc gataaagaac
    12661 ttattaagga agtaattaaa gagattcaaa gagctcttat ttcttcagac gtaaacatac
    12721 aactagttct aaatatctca aaagaaatag aagaaaaagc ttttgaggaa attccatcag
    12781 gaattacaag aaaagaacat attgtaagat tgtgttatga aactcttcta aagtatatgg
    12841 gcggagaagt aaaaactcca cttccagaaa acccagaaac cattctattg tgtggtttgt
    12901 ttggttcagg aaagaccaca actgcagcaa agctttctaa gttctataag aaaagaggat
    12961 taaaggttgg acttatctgt gcagatactt atagaccggc agcttatgag caattaaagc
    13021 aattaagcga tatggtcgaa gttgctttct atggtaaccc taaagaaaag tctgcacaaa
    13081 aggttacaaa agaaggtctt gattatcaca agaaaaacgg ttgtaatcta ataattgtag
    13141 actcagcagg aagaagtgca ttagatgacg agttagtaaa agagattaaa gaaattgacg
    13201 acgtttttaa tcctaagttt tccttacttg taatttctgc agacattggc caagcagtta
    13261 aagaccaagc tactgctttt aataatgcta taggggttaa tggagtaata ataacaaagc
    13321 tagatggttc agcaaaaggt ggaggagcat taactgcctg ttatatcaca aagtctccag
    13381 tgtactttat aggaacagga gaaaaagctg atgattttga aatctttgat tctaatagat
    13441 atctttctag ggttttaggc tttggagacc ttgaaagctt aatggaaaag attgctgaag
    13501 ttacaaaaga agaagaaatg gaaaaattaa aggacataaa tccagaagat atcatgaaag
    13561 gaaatgttac ttttggtgtc tttaagcaac aacttgaatt ttctaaaaaa cttgggcctt
    13621 ttagcaaggt catgggtttc ttgggtgttg gaaataaaat ctcaaacgag caaaaagagc
    13681 ttggagaaaa gaagatggca aagtacaggg caatacttaa ttctttaact aaagaagaat
    13741 tagcaaatgg tcctgatttt ttaacacatt caagaatgga tagaattgct aaaggttcag
    13801 gaacaaagat tgaagacatt aaagaattat cttcacatat caagaaaatg aaaaaaatgt
    13861 ttaaaggaat ggatgctaag aagatggaag gcatggcaaa gcagtttgaa gggcaagacc
    13921 cagaaagtat tgatatgtca aagataaaca tgaaccagtt tgcaaatatc aaaggcatga
    13981 agaaaaagaa attaagatta aagtgaaaaa taaatgtttg agactatttt tgttattaga
    14041 ataattctgt tgttactggc agtggctctt gcagcatatt ttgattataa gacaggatat
    14101 atctatgatt ggataagtat tccgcttatt gcaattggtt taattattaa tatatttact
    14161 tattctttta aaagccttat accaattgtt ctaatttctg caggaatcta tctttttgga
    14221 tactttgcgt attatttcgg aaagattggt ggtggggaca taaagttgtt tattggaata
    14281 catatgattc ttccatatct taataatcag ttacttatct tttgggtaat tattctttct
    14341 tctttacttt cagtaatgtt tgtttcaatt tcttatgctt ttaagcttaa taaaaaaata
    14401 aagataaaca aagtgctttt gaaaaaaaag aaatctaaga tttttaagtc cgcaatttta
    14461 ttttttgtgt ttctagcatt tgttattttt gcaacaagca caggcgatat gccaaagatt
    14521 atttatataa caattccacc aatgtttttt ggtagtataa taattatttt tgaagatgag
    14581 gtaaaaaagt acatttactt aaaacaaaaa ccagtagcaa agctagaaga aggagatgtc
    14641 tttgctagcg agtttgcaac aaaagagcta attacaaaat taaaattagg caaaagaaca
    14701 gttcttgaaa aagaagacat aaaaagagca aaattattaa gattaaaagc tcttccaatc
    14761 tttgataacc ttccaaggtt tggtgtatat atccttcttg gagttttatg cgttttaatt
    14821 tttggttctt tgcttttata aacgcactaa atctgggaca aattttaaag gaaacctaaa
    14881 acatgtataa aataacaaac gaatctgctg gtgctcgttg tggcgagttt aagaccagtc
    14941 atggaaagat taagactcct ttctatatgc cagtagtaac taaaggcgca ggcaagtttc
    15001 taacttttaa ttctttagaa aatttaaata ctcagtgttt tatctctaat gcttatcttc
    15061 tatatcttaa gccaggtctt gaaactataa agaaagcagg cggctatcat aactttatca
    15121 actggcaaaa accaatcttt acagactctg gtggctttca attacttgat ccacagtttc
    15181 ttatttcttt aaatgacaaa ggcgtaaagt ttagatcacc tttctcagga gaaccacatt
    15241 ttgtttctcc agaaagttta atgaatattc aaaatacttt aagttcagat attgctatgg
    15301 ttcttgatca cgttcttggc cctgaccaca caaagaaaga attttctgaa gcaacaacgc
    15361 gaacaattgc ctgggcgaga cgttgccttg acagccacaa gaataaaaac caaaaagtgt
    15421 ttggtatagt tcaaggcgga acatacaagg acctaagaaa agaatgtgct ttagcaatca
    15481 atgacatggg ttttgatggt cttgcaattg gtggtttagc aattggcgaa agtaaagaaa
    15541 gaatgtttca agcaattgat tattctttag agtatcttga caaagacaaa atcaaatatc
    15601 taatgggtgt cggatctcca gaagacatga ttatttcaat tggtaaaggt gtagactgct
    15661 ttgattccat ttatccaaca aagatggcaa gacacggcat gatttttaca agaaacggga
    15721 tattaaagat tggcaaaagc tataattctc atctgtttaa accaatagaa aaggactgcg
    15781 attgttatgc atgtaagcac cacaccatct cttatcttca tcatctctac aggcttgatg
    15841 agccagcagc aaagattctt ctttcagagc acaatgtaag gtttattcat cgcttgatgg
    15901 aagatgcaag aaaggcaata gaaaaaggcg tctttgataa atttcaaaaa gagtttttaa
    15961 aaaattattt gaaaaacaag taaataatac cactatttct ttaaattcac aaaaaaatca
    16021 agcagcgcgt tttatatacc ttgaaaaaca caagataatt acaaatatat aaaaatattt
    16081 tgtatatcaa ttaatataca aatcaaaacg tgattattga tatgcaaagt aaaacaaaaa
    16141 ttgtaaggta tcctagatta gatacattaa tgatgattga aaaaataatt gaagatgcta
    16201 aaggagattt tacagtaaga cagatttgga aaaaacttcc aaagaaagta atgtggcaaa
    16261 catatattac tgcacttgat tatcttgaaa gcattcataa aatagcaata gataaaaaac
    16321 atgtgatttg gatatggggc ccagaagaga ttgcaagagt aaaaaggcaa aaactagagg
    16381 taaagtcctt aaggaaccgg aaccgtattt tatagatcca acaattgaaa aactatatta
    16441 tgaattaaaa gaaagtacaa gatttgaaga taaagaacta tattctaatt taaaaaaagc
    16501 aattacaaag attcaagaaa atccatattg cggtataaga gttccatcaa agctttggtc
    16561 taaggaatac attacaaaat atgatattaa aatattatat aaatatgatt tatcaaatgg
    16621 ctggagattg acttacacta ttcgcggcca acaaatagaa gtaatttcta taattattga
    16681 atggttctgt cacaagaaat acgaaagaag atttaagtat taattattta aaccaaaggt
    16741 ttattaatat ttataagcta tatattttat tttatttaca ctaaagaggc aaagaaaatg
    16801 gcaaaaaaag agaaaacaaa aatacaaaag aaaaaagtaa aatccacaga taataaagaa
    16861 actaaaaaat atttctttag atttgtagaa tcaaataacg cgcagatagt tcttttaata
    16921 ttgtctataa ttttggtagt ttcatctttt gtaaatacca ttttctgtta tttaggtaaa
    16981 ggtggagatt ttggtagtag ttttttttta tttgtcccaa gtgtaatttt attactttat
    17041 tgttttaaat ctaaattagt taaggtattt tcaaagatgg taaatatttt aactcacata
    17101 ttaatagtgt tatatttatt atttggtatt tatgcatttg gcaacgatat aataatagat
    17161 gtttttttgg caataatgtt tagttttatt gtgttctatt taattttact tatatttaaa
    17221 aatcagaaga ttatgaagta ttcacaatta tttaatattg cacttttctt ttttattcta
    17281 gggctattag caatcttatt gattcaattt atgaatatta atttataata gcccgctttc
    17341 cttaaaacta aagtatccgt tttctgtaat gattagatga tcaatcaaag atacatccat
    17401 taattttgca gcctttccca gctgttcagt tgatatcttg tcatcagttg atggttcgca
    17461 gattcctgtt gggtggttgt gtgcaacaat aattcctgat gcttcttctc taatggccca
    17521 aagcacaata tcttttgaag aaatcaatgt ttggttgctt gttccagtag ttaaaatctt
    17581 acaagagata acttcgtttc ttggagaaat aaaaacagca atcaactttt cttctttaca
    17641 atcaagaaac tttggtaaaa gatactcaaa tgcatctctt ggggaagtta tcttctctgg
    17701 agacttaagt attccatgtt ctctaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnngac atttctttta ttttcattgt tattcatttc cccttttttt attatccaat
    17881 atccaccatt atcagggcca actctttcaa taattgcttt tgattttagt tttttaatgt
    17941 tgtttctaat gtttttttcc gtaatattta taatttcaga taattctttt tgagtaatat
    18001 ttttattttt ttgtatttca ataattattt gtttttgatt attatttagc tctaggtctt
    18061 tatctaggtc tttatctagg tctttattta gaattagact tattggttta tgtaatataa
    18121 ttgtaaacca attttcagaa atatcaaatt cacattttaa attatattct ttcattgcct
    18181 tttttattct ttcaattcca gttcctgcca tttctcctag catgatcttt gccattaatc
    18241 caaataataa aggatttcta ggaacgcttt ttttcccaaa ttcttttttt gtaattccag
    18301 agatcaggcc ccctggattt gctatttcta ttctattcaa ataaatatca acttgaatgt
    18361 gcccattaga aaaataatct cgatgagcga ttgcattaat taaactttca gcaattgctc
    18421 tttccggtat ttctagtttt tcttctctct ctctgccggt aagtatataa ttagtattta
    18481 gttttgaaca aatgtaagta aaggcgttat gataattaga atataagtct tcttcaaatt
    18541 cttttttgtc tagtatctct attttattgt ttcctttata taatacacaa gttatttttg
    18601 cagacacaaa aaagttagtt gttgttttac aaaaaaataa aacccctgca ttttttagat
    18661 gttttccatc aaaaatagac aaattttgta gtatttgttt aattggcaat atgttactta
    18721 tttttgcttt ttgaagaaat tgaattaaaa caatctgatt taaatcctca gataatttaa
    18781 aactgctatt gattttttca tcaaatctta taagcccttc attttgaaag aattttctta
    18841 attcttctct tctcatcttt tgagagtttg caccgattcg taaataatat tgaccacaat
    18901 tagaataggg tttttctttt ccttcaggta cttctatgat tgtaattttt gattcttcat
    18961 ttactttcaa ttgaattgta gggtcaatat ttcttcctat atctattatt tgagatttaa
    19021 gtttatttgt taatctaaat ccaataacct gtccattatc tttaacacca agaattattc
    19081 gcccacccgc gccattagca aacgcacaga tagtcttacc aatattatct gaaaaagctt
    19141 ctttaaattc aacagtgtag ccttctccag ttgcaattat tgttttaatt ttcaatttca
    19201 caatagtttc ctcggattat ttgttttagt taatattgtc atttgttcaa ttgcaatatt
    19261 gtttaatttt tccagtcttt ctaatttatt taatccttct ttaataaaaa cagcattaag
    19321 attttcaaga ttagataaac aaaccagttg agacacatct gcatagtctc taatgtttcc
    19381 tttctctttt ggattttttt ctttccagtc tttagcagtc attccaaaaa gagccagatt
    19441 aagaatatct gcttctgttg cataaatgat gttttcttta gtttttgaga tctttgatgg
    19501 aatcagatta ttttttattg catcagtatg tatatgataa ttaatctttg ctaagttcct
    19561 tttaatatcc cagtcaagtt taagtctttg actttcatct atttttaatc tttgaaattc
    19621 tttaattaga tataatctaa attctgcgaa aatccaagat gcaaattcaa aagcaatatc
    19681 tttatgagca aatgttcccc cattgtgtcc agattttgaa ataatcccaa tagcccctgt
    19741 tttttctatc catttttgtg gaggcaatac aaatgcattt aatccagcca ttttaagaaa
    19801 ctggtcgaat tcgaccagtt tgaaatctgg gttatttatt ctttcccaaa gtcctagaaa
    19861 tacaatattt tcttttgatc tcatccaatt tttaactacg tctgcaggat gttctggatt
    19921 tttatatttt gcaatgtctg taagggatat ataatctcct tcgttttttg aataataaga
    19981 tactcttttg tttaaaacag ttatttcatt catttaagtc accaaaaata tatgtgcgtt
    20041 ctaaaggggg gtatttttgg aggggtagct gtccgaccct aagctgattt atttaattag
    20101 tgtctgggaa atatcaatta atctacatct tcaggaagtc atttgtaaat ttgaaatgcc
    20161 agtgcgagaa atttcaaatt cacaaaaatt cgttttttta aacagtttag taatctttat
    20221 atattattat tattattatt attattatta tgataacaaa caaaaaagca atcactccaa
    20281 ttgttgcaag cattttatta attgtagttg cggtgatttt agttactata gttttaaact
    20341 ggggcagaag ttttagcaat tcttctttag caactgcaga cacagaaaag ctatcaaaat
    20401 cagatgcttc agagtttgtt tacaccaagg ccttttcaga tggtgttatt caattcaact
    20461 atgctccgcc agatagcctt acaagtcaaa acataataat aacaggttat aaaatattct
    20521 cagacaataa cgaaactgca gaaattgttc tagattccaa tcacacactt aggcaaggcc
    20581 taaatgttat tcctttaaca gatttctcag atcaaaacat aacttcaaga aagatgaata
    20641 tacaactaca tacttcagat ggcaaatata taactttgaa taacgttact aataataatc
    20701 cttatgttat tcttgaactc ccatatatta tgaacggaga aaccaaacta tatattcatc
    20761 caacagataa ttcaacaggt aatattacat ggggttgttc tggtacatta ataggtgccg
    20821 gggcccaatc agcaacagat ggtgcaacaa atactgcagc aattatagcg ggttgtgcaa
    20881 caagtccaat agctgcaagc gtgtgcgaaa acctagaagc ttatggctat gataactggt
    20941 atcttcctgc aaaagatcaa ctagctgcaa tttggaatgc ctgtactgaa gtcactaaat
    21001 caaatgcttg tatgaatgct gcaataaata aaggggatta tacagaagaa tggagtaata
    21061 ttgtgaacga tttatattgg tcaagtacgg agacgtgggc gggcggcgct tacggtatcg
    21121 atatggatgg cgggcacttc ggcagcccca ataagaccga taccagctac gtgcgttgtg
    21181 ttcgtgacca ataaccatag tttttgacat gctctagcat gtcaaagaca acaagacatc
    21241 cagcggatgg cttgctttgt ggttttagtg aaagacacaa cccagcgggt tgtggctttc
    21301 tgtttgaaaa tttttaagtt ctttttcttt ggctgcgaaa tcacttttat aaaaggagtg
    21361 tcactggaca gccaccccca aaacatatat atagtgtttt gaacttaatt ttattatgag
    21421 atactcacat tcaagaatag agacttacaa aagctgccca aagaaattct atttccaata
    21481 tatagagaaa ccagatattg aggaaaagaa aggaattgag gctttcttag gttctatggt
    21541 tcacttagct ttagagaaac tctataagga tctaaagttt acaaagctta attctcttga
    21601 agagatacta gaatactatt cagaagaatg ggaaaagaac tttgatctaa caatagaaat
    21661 tgtaagactt gattacactg cagagcacta caaagagatg ggcaaaaggt ttctaacaga
    21721 ttattatgag aaatatcatc cgtttgattc aggaaagact ttgggcctag aaatggaaat
    21781 aactttaagg tttattgatg attatggtac gccttttgat ttgattggtt atatcgatag
    21841 gctaacaatg attaatgaag aacattttga aatacatgat tataaaacca acaatagtct
    21901 taagactcaa gaagaagtag ataaagacaa acagcttgct ctttacacca tagcaataaa
    21961 gaaaatgtac ccaacagtca agagagtaga cctagtctgg cattttctag catttaatat
    22021 tgaactgcgc tcttcaagaa gcgataaaga tctagaagaa ctagaaaaag aagttgttaa
    22081 agctattcaa gagattgaaa gaaaacaaat aactaatgac tttccaacaa aagaatctgc
    22141 tctttgtgat tggtgtgctt ttaaggagat ttgtccagtt aagtctcatt cttttaagtt
    22201 aagcaagctt ccagtcaacg aatacctaaa cgacgatggc gtaaagctag tagaggaata
    22261 cctaaaacta acaaaggaaa agaaagaagt gctggataag ctcgacccag aactagaaaa
    22321 actaaaagaa gcattagttg cttactccaa aacaaacaat tctgaaagag tgtatggccc
    22381 tgaaggcaat tcaattcttg taaaggaata tgaaaactat tctcttcctg agaaagattc
    22441 aaggcaaaga caagatttcg aaaaattact aaaggaaagc ggtacttggg aaatgatagc
    22501 agaagttaat tcctttaatt tctcaaaagc agtaactagt gggcttttct ctaaagattt
    22561 cctaaaaaga ctagaacctc tgatttccaa aggcaaaaca acaagacttt acccaagttc
    22621 taaaaagtaa tatttataaa ctattttctc tttatcttta gtaataatta taagtttata
    22681 aggaaacaaa atgtcagaaa aagaccaaat caagattcat tctataaaag ttgattcttc
    22741 agaaaataaa aacataaaag aagcaccaaa aaaaacaaaa cttatacatt tatcggtctc
    22801 tggaaaaagt gagaaggata taacacaggt aaaaaaagaa gttaaaaaga aattaacatt
    22861 taaggatttt gtttttaacc tttctaaatt aatcaatagt attcctaatt tttttgcaaa
    22921 tttttttgat acttctttta gaacaatcgc atatacaata gtaggaacaa tgctaagttt
    22981 tggagcagca cttgttttat tccacgagaa tgcgggttta ttttctgtat ttttcttaac
    23041 tatttttctt gcgccaatag caataaaaga aacaaatcaa aatgttttat tggcaggaag
    23101 aactcaaaaa gttgaagaaa aaggagtttc tcttacaaaa ctaaaagtaa aagaatctaa
    23161 gaaattttat ttaaaggatt tatttgaaga aaataaaaaa actctttata tttatttatt
    23221 ttttttctta ggaataatgc ttgtagttat ttctttaatt gcaattatgc ctgttgattt
    23281 ctctgcacaa ttgtttagtg agcaaggctg gaatacagca cttatgccaa ctagaagcat
    23341 tggttttgca ggtttagaaa aaacaagtat attctttgat attttaaaga ataatttctc
    23401 agttatgctt gtttgtttta ttattgcgtt aatctttcca ttaggcgcat tacttatgat
    23461 agtttggaat gctatgtact gggcggtttc ttttagtcag tatgctttat tctatagtaa
    23521 agtatatagt gtgaaattac tctctattct ctttccatta ttgcttagtg tttctttaca
    23581 cacaattata gaagcaatat gttatttttt tgcagtaatt tctggaacaa tgctttccat
    23641 gagtattaaa tctgagaagc tagaatctga tagatttttc tatcttttta aatattgtat
    23701 aatattatta gcattttctg tatttttctt aattttaggt tcttttgttg aagttttctt
    23761 attcgacggt attaaaaact tcttttttag tttattctaa gacctttcta aaaaaatacc
    23821 tttatatttc tttttttacc taaatatatt agttattcta tttatatcta ggaggaaaaa
    23881 aacatgacta caaatgacaa taaagaagag tcaataatga gtccacatca ctcaaaaaag
    23941 agaatactta tttggcaatt gattagtgct gttcttttta ttgctttaat aatagcaata
    24001 ttacttgcag taaccaataa gccagaaact accaattcgc caatgaccgg aaatattggt
    24061 ttagtaaatc caacaacaga aacaaaaatt aatcaaaagg tagttgacga tactgtttct
    24121 tttcttaaag aagcattcca gttaccagaa gtaacggtta aagaaacaaa agtcgttgat
    24181 ggtctttatg agataacaat tacaattgaa gatcaagaca tgccaatcta caccacagca
    24241 actggagaac acattattgt tccgggcatg ggtcttgtaa acaaacaaga agttttagta
    24301 caaatggaag aagcaaaggc tttagaagaa gcacaagcag aggctgaagc aaatattgca
    24361 aagtctgata agccaaaggt tgaagtcttt gtaatgtctc attgtccata tggtactcag
    24421 atggaaaaag gcttgattcc tgttatggag gccttaaagg ataaagcaga catacagatt
    24481 aagtttgttg actatgcaat gcatggagaa acagagctaa aagagcaact taatcaatac
    24541 tgtattaaca aagaagaacc agagaagtat ctaccatact taaagtgctt cttagaagca
    24601 ggaaagacta cagaatgtct agcaagtaca aacattaata caacaaaagt caatgcttgt
    24661 atttctgcaa ccgatacaga atacaaggtt acagagttat ttaatgatga gtcaacttgg
    24721 tcaggcgggg cttatcctca gtttaatata tacaaggcag aaaacgagct ttacggcgta
    24781 caaggctctc catcaacagt aattaacggt gttcttgtaa gcaatatgcc aagggattca
    24841 aaaggaatac tagacaaggt ttgttcagta tttaacacag caccagaaga atgtaacaca
    24901 gctttaagta gcgaaacacc aagcgcaggc tttggttggg gcgattcagg agcaccaaca
    24961 gatgctactt gttagtttct atttcttctt tttttcttat ttttatctca aaacccatac
    25021 caacacattt aacttaaaaa ccatactttt ttaaatttgt gttggcatat attatttatg
    25081 gtttcagtaa tagaaaagaa aaatggcaca aaaaaagcct attatttaca tatgacttta
    25141 agatctaaaa ctggctataa gaatcttgat aaatatctag gtataaagtt accagcagat
    25201 cttagaacaa aaaaattaga tttctttatt gattctcaac aagggcaatt agaaaaaatc
    25261 tcagaacaaa tatccaaatc taattctcaa aaattaaaaa tgccaaaaac agttcaagaa
    25321 aatcaattgt ttgattttgc agtaaagttt acatataata ccaacagaat agaaggcaac
    25381 accctaagtt taaaggattc ttatctttta cttagagatc agatatctcc tcaaaaatcc
    25441 atgcgagaca ttaaggaaac agaatctcat cagaaattat ttttagagct tattaaaaag
    25501 agccagaaca ttacttatca aaatctagta aattggcatt attttttatt taaagacaca
    25561 aaaaaagaca ttgcaggaaa gattagaaac tatcaagtag gcatatctgg ttgtaagttt
    25621 attccaccaa gcccagcaga gctttctgca gaattaagtg gtttctttaa ttggtataat
    25681 aaaaataaag aaaaatataa tcccgttgtt cttgcaggat tagtacattt aagattagta
    25741 acaatccatc catttggtga tggtaatggc agaatcacta gactattaat gaatattatt
    25801 ttaagtaaca acaactatcc tcttttcatt attgattata agcacagaaa aagttattat
    25861 tcagcactag agaaatccca aacaaaagac gacgattctt atttcttaag ttggtttatc
    25921 aaaagttatt tgatttttat aaaaagcaaa taactaatca atacccaaac ttatatattt
    25981 ttataagttt cggtattata tatatttatg aaaataaccc tctataataa attaaaaaaa
    26041 caacagcata gagatattgc tagtcttcaa gacgagctag ttaaaattat ttatggcata
    26101 tttccagagg cggtatttca tggaggcaca tcaatctgga gatgctatga tggaaacaga
    26161 ttttctgaag atatagatct ttatttttat gaaaaacaag atatagaaaa gcagcttaaa
    26221 gaagagctac aggggaaagg cttaaatctt ataaaattta aaaaaaccga aaatgttatt
    26281 tttgctaaga ttacagataa gaatatagaa gtaagattag agataaggtt attagaaaag
    26341 agcaattcca ttttcaaaac aaaatccata aaacaatatc aaaatctaga tggatcttca
    26401 ataacagttt tttgcctctc tcctgaggaa ttacttctag aaaaagctct tgcttacaag
    26461 aatagaaagc tcataagaga tatttatgat gtttatttcc ttcaatcttt agcaaatctt
    26521 tcaaatgaac aaaaaaagaa actaaaagag atagttgatt cttgggaaaa accagaagat
    26581 gagaaaaatc taaaagcaat agtatattct ggagcaatcc ctagttttgc tcaattgctt
    26641 tctgaaatca agaggagata ttaatgacct atgaaaacag attaagagat gtatataagg
    26701 aagaatataa atttacaaaa aaagatgctt ctaaattatt ttctaaagac aaaattagta
    26761 atgaatattt agatcaattg ttgcataatt tagtaaagca aaataaaatt attagaataa
    26821 caaatggttc ttatactttt aaaaaagatc tcatggtaag tggttttgct tattctccgt
    26881 tctactatgg tcttcaagaa gcattatctt taagaaacct tcacgagcaa gaaacaaacc
    26941 ctataattat aacaacaaac aagataaagc caggaataaa aaccatacaa ggaagtaatg
    27001 tattacttag aagaatagat tgtaagtatt tttttggatt tgatttcatt aaatactatg
    27061 atatggaaat cccggtttct gattatgaaa agacattaat tgattttgtt tattataatg
    27121 agccattatc taaagaagta attaatgagc ttaaaaaaaa aatagataaa actaagttac
    27181 agaattattt gttaagatat tcaaaaaaga taagagctaa agcaaattta cttctaaagt
    27241 agtttattca agaatatagt ttttcttaat ttcttctaaa gcagcagaaa caaatttttc
    27301 aatttctatt ccaagtgtct tttcacacaa gtatatctgt tctctattaa cagcagctgc
    27361 aaaatacttg tcttttagtt tcttgattat agatttagtt tctgctttag tgatgtcttt
    27421 gtcaggtctc atcaagacat atgctctaat gattcctgtc agcccgtcaa gagaatatag
    27481 cgcatttctc aagttgctat ttctctcaac accaataatc tcgttatggc ttttgatgtc
    27541 atcaatcagt tctttatcaa cgttcttctc aagaagaatc ttttcagtaa caagagtatg
    27601 ttctttgatg tcctcattaa agctttcaag gtcaaggtca tgaagtagcc caatcacaaa
    27661 ccaatagtct tgttctgttt ctgagagatt aagaagttta gagattccaa gaagtccata
    27721 agccacaaga aaggaatggt cgatgttagg tttttcttta agatgttctt taagcaaaac
    27781 cattgcttgt gttctgttag gtatcataag ccatcactta taagattgat ataaggcgtt
    27841 gatatcagaa tcagaaaggt tagtatcaaa cactctcaaa tactttatgt ggccgtttcc
    27901 tggcataggc aatccagcat taccaataat gtgtggataa agattctgat tttcaggtaa
    27961 taaataagtg gtaattgtag ctgaagaatt gaatctaaaa tttatttcag ggaatgtaag
    28021 atttgtggga tgtaagtaaa aagattggca attgtccagc gccccattaa tatacataca
    28081 aaagttatca tcaattctta ctattgcaat ttgagaatcg ccagttacgg ttccaataga
    28141 ggttaaggtt ttaccattat atccctgtat cccaaaataa ccgctttttt cttgattttt
    28201 aatattttca agatcagaca tcgctctttt tacaattcta ctattagaac tatcagcaat
    28261 ataaataaaa ttgtcgtcta atgccaatcc ctgtggtaaa taaaggccgt ctttttccca
    28321 gttaagtctt ccgaatttat aaataaaatt aagatttgta tcatatactt taattctgta
    28381 atttctagaa tccgaaacat ataacgtatc attatatatt gttattcctg tgggttcgta
    28441 aaaagaatct gttgttgaac tcgcattaac ccctaaccca tacattttta ctaatgagag
    28501 atcgtttttg tttcttttgt gtattctata atttgacagg tctgtaacat atatataatt
    28561 atcatctatt gttatatcag ttggattatt aaattgatca tctccacttc ccgaaacagt
    28621 accaccaacg gatgccacaa atgtaaatgt gtcttttgta aattttttta tgcgatgcca
    28681 agatctttct acaacatata tataattatc atctaccgct atgccaaaaa tatattgtac
    28741 catttcattt gaagagccac agccattaga taatttagat acataagaca aatctgtttt
    28801 gtttctttta actatgcgac aattggcatt atcgctaaca tatatataat tttgatcaat
    28861 cgctattcca taaggggaat taaattgatc attcccactt ccagaattag gtccccccac
    28921 agaatataat gtagacaaat cagatttatt ttttttaata attctgtgat ttcctgtatc
    28981 tgaaacatat aaataatttt catcaacagc aaccccccaa ggattgtata ccccgtcatt
    29041 attataagta gataaattta tattagttat atagcttaaa tcagacgggt ggtgtatttt
    29101 taatctatca ttgccagtat ctgcaattaa taaagtgttt tcatctttat attttatatc
    29161 tatcgggagg taaaagtaat tgttgtcggt tccactagaa ccaaatttag taacatatga
    29221 taaatctgat tttaaatgtt ttttaattct attatttgca taatctacaa cataaatatt
    29281 attttcatca agggataaat atgtaggact agaaaactga tcatttccag aacctgagcc
    29341 tgttcccccc agacgtgtca cataggcaaa agtatttttg tctatctttt taattctgtt
    29401 atttgtatat tctactgtat aaatataatt tatatcaact ccgactccaa atattccaga
    29461 aaattggtca tttccagttc cgttagttcc aaagtgtgtt atatattcca ttgtatcttt
    29521 atttagaatt acaatccggc tattcccata atcagcaaca tataaattag tatcgtatat
    29581 tgctaattgt cgtgggccat ttaatgtggt tgttgtggcc ccattagttc cggttccaaa
    29641 ttgtgaaata taacttaaat cagatttatt aaattttata actcttggag acgttctatc
    29701 agaaacataa atataatctc catctactaa aatataatta ggatagttaa aaaaattact
    29761 tcttattgct tgcgtggttc cactagcaac accatacgga ccaccacgat atgaaacaat
    29821 ctgattagtt gtcttgtcta ctttttgtaa tctgtgtata attgaatcaa taacataata
    29881 atagttttca tcactatcta ccgaatatgg agaataaaag aaattaaaat ctattccaga
    29941 acccacaaaa gaaatattca catcaaattc tgaaagattt cttttaatta taattccggt
    30001 tgttggatca gcaacataaa gatagccatt atctattgtt aatccgccag tagataaaaa
    30061 ttcagaatag gcatatcctg ttgaataccc tatatttgag atataattaa aattggtatc
    30121 atattttaat attctagcat atccgttatt tgaaatatat aagtaattat tgtcataaat
    30181 aacatatgca gggttataga gtgtgtttgg taaataattg ctataccctg tcaatccgag
    30241 ttgtgaaaca aatgataaat ctgattttaa atgtttctta attctttgat tagtataatc
    30301 tgcaacataa acatatgtat catcaacagt aactgaatag ggattactaa atgtgtcgtc
    30361 tgttgagcca gtggcccccc caatatttgc aacaacactc cacggagtcg aactaatatc
    30421 tatttttttt agtgtgtgca aagtactacc gtagccggta gtaacatata aataatttcc
    30481 atctaccgcg attccataag gataacctag tgttccagaa cctgggctat aggtatatac
    30541 ccaggataaa tcagatttgt tgtatttttt tacttgatga tattgtctgt ttgttacata
    30601 caaaaattca tcatcaacag ccaaatcctc tggataataa aaagttgtac ttgtagatcc
    30661 gggatttggg cccccaatta tgcctaccac tgccaaatca gataatcttc tttttacaac
    30721 ccggtgtaaa gctttgtctg tatagtaaat atgttcccca tcatttgtta ggccgcttgg
    30781 tacaaaaaca ttttctggta aggggtcagt atcagaaaat atttcgtaaa cagaataatc
    30841 tgattttttg atttttttaa ttttattatc tgtggcggtt aaaaggtaaa gataattttc
    30901 atcatcagca atatctgttg ggttataatg atagttatca tttcctatcc cccctgttga
    30961 agagataagt gcgacataag ttaaatcatt tttatttcta atcattattc tgttgtttgt
    31021 gtattctgca atataaacat aattatcatc tactgttaat cccttaggtg cacgcatttg
    31081 atcagttccg gtaccataac ttgttccaca ggcttttgaa acaaacgata agtccgtttt
    31141 gttatatttt tttattctat gatagtaatc ttctgtaaca taaatataat tttcatcaac
    31201 cgctatgcct ttaggattat aaaattgtaa atctccaatt cctgatgaag taccgccaag
    31261 tgtagcaact aattggaatg tttctttatt tagtttttta actctgtgat atgtcgcatc
    31321 aataacataa atataatttt catcaactgc aatgcccagt gggccattta gttgattact
    31381 tgcagatcct gggccattag ataacattga atgataagac aaatccgatt tgtttaattt
    31441 ttttattcta tgattactat aatctgaaac ataaagaaaa ttatcatcta cagcaagagc
    31501 ataaggcgca ctaaaagaat caattgcatt acctaatctt gggccaccaa cagtagcaat
    31561 cagcttccag ttatcttttc ttcgtttttg aatactttgc gtaccagtaa ttgaaagata
    31621 tatatattcg tcatcatcag cgaccccata tacaccccat agttggtcac tacctgagag
    31681 tttaaaattt aaataggaat tttcatctat aaacagatta attcctccaa caaaacctct
    31741 ttgactgtaa atgtgtccct tataattatc tgttacaaat gcctctaatg tgaaattatc
    31801 gacccctaaa ttaaaaaaag gattagaatt attccagtcg ttttcaatat agatataatt
    31861 attagaatcg tttgtaaaat ttgcaccatt ttcatcaaac aaaactttgc tttctataac
    31921 tgataggtta ttatctgaga gatctgttgt tgtgtttgta tcatatttat tataaaataa
    31981 tacccccatt atttcagtaa atatctttgt gataatttct gaaggattat atgcatattt
    32041 atatgccctt aattttagta ttgtgttgtc atctgtaatt gttatgggcc ccacaaaagg
    32101 aatagaattt tctgtaggat cgttgccatc taatgtataa tagatatttg catctattgg
    32161 ttctatattt gcagttacaa ttaattcttt agtaaatgca tttccatcag gcaatatatt
    32221 aaaaaaatta aagtcaagaa gttcaagacc aataaattgt tttgatataa ttgcgctcgg
    32281 gatatagcca tcagcatagc ctcttgcttt tatggttgtg ctgtctgtta tcgtaaatgg
    32341 ctcagtatat aatgtagatt cctgagttgg ctcgctactg tctgttgtgt aataaatatt
    32401 tgcgtctgtt ggtgttgcag ttaatattat ttctaaagaa ccaataaaag tattaaattt
    32461 tgggctaatt gtcaaataca cttgcccaag atattgcgta atagagataa ccggaatgcc
    32521 attaataata tcagaaatag tcaatcttag ggtatagttt ccatttatta ctatagtttt
    32581 atctaaagta aaaattccat caagatataa agtaaaagtc attagtccat ttgctttgac
    32641 aattgcaaga tttccataat tagcgtcttt gaagttttta aaggagttat tagatatttt
    32701 ttgcttaata taaactgtgt ttgttgtagc gtcaaaaaca atttcgtcta caacatcaag
    32761 atttagattt ttaaaagtat taatgtcatt tgtctttaaa tctaataatt cttggtttat
    32821 caaaatcaag ttttctttat tatgattaaa atttatttct tctcttagag aggtaaagaa
    32881 tggcacaact aagccaataa taattatcat tgcagcaaca gataataata ttatcataat
    32941 actagtaaaa attacatttg cgcctttgct attcatgttc tgcactccat gcactctaag
    33001 ttaattaata tctgatcgtt ttcatcatca tattcttgat attttatgtt tagtgttgtt
    33061 ttcttattag atataataaa atcattacaa tcaagtatta gtcttttata attatcaaga
    33121 ttatattcaa aatatattgt gttattgttt ttataggtag ctatttcgtt aatcataaca
    33181 atttcgtctt tatatttgcc aaaatagtct atttctccag taatcaagtt gtttgtacag
    33241 tcaattatta tttctcctgg gtttttaacc actagctcta tgtctttttt atcgtctacc
    33301 acgtcttcga ctgcgatttt tagagtagtc atgctttcaa gcattgtatc ataattgctt
    33361 tgttctttaa cgccatctaa tagttccatt gcagaatagg taactacacc aagtataatt
    33421 attgcaataa gtatgtaaag taatgtgcta ataactgcag acattccttt ttgtgttttt
    33481 attttcattt ttattacttc tttgttaaat tctttcccta aggaaagaaa aacgcgccta
    33541 tttcctttcc ttgaggaaag gaaacgcgca ttcatgattt ttcaagtact cctatactta
    33601 ttgttgcggt attgctatcc tcaatggagt tatcacttat attagcatca ggattaccaa
    33661 agtagatata gattggagta tctgttgtag attttgtaaa atcatatcct gcaatatttt
    33721 ctacaatttc attagttcca acagaataga atgtttctgg agaattaagg ttattatatt
    33781 ctgtttgtat aaattctcca gataaaatat cattatatat tctaaattct tttatatctc
    33841 ccacccaaac gtgtgttccc gcagtagaat atattccaat tttttttgta ttattttgat
    33901 taggtaacaa aagtttgttt gtatttacag tgttaattaa gcttccattt ctgtaatatt
    33961 taacttgaga agcattttca tcgtatttta ctgttgcaac aatattcaaa tattgattag
    34021 tataatttaa gaaataatta gaagaagtaa tatttataaa attgctaacc ccatcagtat
    34081 tttgcatata taaactattt ccacctttaa atatccaaat aaaaggggta gttccttgcg
    34141 tacgatcatt taataaagaa ttatatgatg cagagtttgg gctgtctact tttgcccaaa
    34201 aataataggt taatgagttt gtatcgcttt ctaaagtaac attatcttgt gtaatgatat
    34261 tttttgcagt agtattatta ggaaaattaa aagatttatc tacattatca aaactaatta
    34321 gactttgatt aaatgtccag gaagtatctc ctgctaaatc agtaatggta tttccactac
    34381 caggatatga attcctgttt tttgcattaa accaaagact caaatcttca taatttatat
    34441 cattaagatt attttcttct gaatctccaa aattcagata tatttctgta tctgtagttg
    34501 aagagagttc tggtatttcg attcttgcaa aaagtacagc tgttttttct aattctccta
    34561 cagcagaaaa tgtttcgggg gctgattgat tattatattc tgtttttatc catgaagttg
    34621 tacgagcagt atttgataat cttacttcat caatatttcc attaaatgct ctttttacgg
    34681 gagtattcca cgcaccaata tataattcat tatttgggaa atcaaatttt atttgtttat
    34741 taatgtaatt tctactttgt attaaatttc catttatata aaaatttaaa tcatcgccat
    34801 cgtaagttcc tacaaaatga tgccaatcat tatcagttat aggatattcg ttagcataaa
    34861 ttgaaccata agtattatct gaaaatccta ataataaatt aaattgatta gtgtcttcaa
    34921 tatggcgtat tatataagaa aaataaggga cagatgaagc tctgccttta tttattaaat
    34981 aggtgccatc agtgtgactt cccgctccgt ttagtttacc ccacagctca atagttatta
    35041 tttgagaatt atatgcagat ttatttcccg tatttatata atcattactt ccatcatagt
    35101 tttgagcata acctattttt ccaattattt ctgatggttc gagagttgct ttttttgtgc
    35161 cgttattact attacttgta ctatctgcta ttgtcgtggt agttaaatca ttcttatgcc
    35221 aaactccaac aaaattacta tcccaaacat ctgtatcatt agtattattt tcttcagaat
    35281 ctccaaagta catatagata tctgtgtctg ttgttgaaga gagttctggt atctttaccc
    35341 atgccacaag ggctcctgtt tcagaattat aatcttcaat ttctcttctt agtcttgtaa
    35401 tttcgtcatt tgttgtaaag tagatatcgt ttccgtctgc atttgctcca tagcttacta
    35461 aattttcatc tacaatacta ataagcattg ggaaatcatt taaatctgaa tctaccagat
    35521 tatgatcaat tgttattttc tttctataaa tttttgaaaa gctttcaatt tctcttttta
    35581 gtcttgttgt gccgtcactt gcagtaaagt aaatatcatt accatctgcg tttgctccaa
    35641 gatttgctaa gttttcatct acaatactaa taagcattgg aaaatcattt aaatcgccat
    35701 ctacttttgt gtgatcaatt gttatcttct ttctataggt attatcatca taaacaggat
    35761 tatttatatc tggaccataa ttaaactgtg ggcctttaat aagtactgac agtgcgccag
    35821 tatcagaatt gtaatctaca atctctcttt tgatttttgt tattccgtct cctaaagtaa
    35881 agataatatc tgaagcgtca atgtttgcat aagacataag atcttcatct tctaaatcca
    35941 attgcacaga cagattattg agatcaccaa caagatttcc actattaatt aatatcttct
    36001 ttctatataa ccattctcct gcattttcat cataaacata ttcgttcttt tgcgtatacc
    36061 aagaagtgct tgtttcatta tctacagaaa cagaaatctt attttctgta atcttcttaa
    36121 agacaagcct tgtctgttct ttgttactaa gagtaatgct tctaatcaaa tcaatatttt
    36181 caaaagcaac accagaacaa atcttttgca gttctctata aacatatttg ttattatctt
    36241 gttctagtct tttaccatca gcaaagaagc ttaggttttt accaacagag caaatctcta
    36301 ttgtttcagt atcgctatcg attgttagct caagattaga aagagagaag ttaatttctt
    36361 tagttgcttc aattggggaa ttatatattt ccataatttc agaaaagacc atgtctctaa
    36421 ctttaatgga attatcataa tttgtttttt ctttaccttt agaaatttga gagtttactc
    36481 cagttattat tattccaaca acaataacca tgacaagcat aataagtatt gtactaatta
    36541 tctcgtttac tgcttttttg ctttttatca ttattcatct ttattaaata tattttagtc
    36601 tataatatat tatatattta gtattttaaa tgttttgtat actaaatatt atagaaacgg
    36661 ctctattttg tggtccgaat agtgtagtgg ttagcataga ggactgtgga tcctctgact
    36721 cgggttcaaa tcccggttcg gaccc
//
LOCUS       DAOOON010000007        35734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_1382_length_35734_cov_23.696320, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000007 DAOOON010000000
VERSION     DAOOON010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 35734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35734
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_35734_cov_23.696320"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2055)
                     /locus_tag="PLK55_01845"
     CDS             complement(4..2055)
                     /locus_tag="PLK55_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOZ35707.1"
                     /translation="MVFVNTVIQKNGETEEFSKEKIAIMLFETMCNYGDPNLNLAEKL
                     AFLIFDKVNAKYSKEIPFEKLDEIIKEILHQENQADLLQAYIFKKATPDTIPSDSEIK
                     ELILKLILNIQETDSLPTKLLNIQAHLNKLQNNQHEKTNKKIFEYIREGVFYPSSTFL
                     RDITLNQQVLSGYSFQIDDTIESIFDTLSQSSIVQKYGSSISIDLSKIRSEKEKVKSI
                     NRNACGPIRIIDLFVSAKSMVGYNINSSNNLYYISIEHPDIVSFLNYSATQNEDNFAI
                     LIPDRFMTAVSENTDYTIDINSEKKQKISSLALLDLISSKIITGEKISLIFVDTINKK
                     NPFLEDYKKFTISPVGMQPVFENASFVSGVIDVSKFVNFLGNTKTFDWLKLKNVIWDT
                     IEFLDNTIDVSKTINEKYLQELKETRSIFLSITGFYTLLTKLDIPYNSDDAINFAYSL
                     SDYINYYSKLKSTELAKERGPFLKFIKSKYELSNFSFEKAPVQKTLFSTDLTKSKKLI
                     KNMPKIDWIALRNNIKKYGLRNSSTFSIIFSDFYSIANECSLGVDAIINYKPIIWING
                     HKFEKINKELLVNVSDLKATWTKQEIESTIPKDIKLNFPLAKDVSPQFFIRLQSIFEK
                     NTDGVVDIKIYFTDNTNIQEIKNTIIEAHKIGNSVFIPEKLNGADKDFNTENKKGLMS
                     L"
     gene            complement(2068..2733)
                     /locus_tag="PLK55_01850"
     CDS             complement(2068..2733)
                     /locus_tag="PLK55_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOZ35708.1"
                     /translation="MSLRIGAIEKFSTIDYPDKISCVIFLKGCNFFCGFCYNKQLVLP
                     ELLDTKPDLSEKEIQDFLDARKGLIDAVVITGGEPTIFGDDLIKFIKQIKDRGYLIKL
                     DSNGSNPEMLKKTIENNLVDYIAMDLKDVFENYNKYTNVPTENIKLSFELIKDSNIPH
                     EFRITTHPELSIESFNKIIDFSRGQKIFVQDFMNTNTIKEYKDYKTIYSQLLKDSKDY
                     ILR"
     gene            complement(2746..4916)
                     /locus_tag="PLK55_01855"
                     /pseudo
     CDS             complement(2746..4916)
                     /locus_tag="PLK55_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466003.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
     gene            5151..6002
                     /locus_tag="PLK55_01860"
     CDS             5151..6002
                     /locus_tag="PLK55_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOZ35709.1"
                     /translation="MDEKNIEILKKFENELNIIGYSQKTIETYLIYLNKFSEFIKKDL
                     KEVNSDDIVNFLSYLKTKKNSGSATMNLVLSAIKHFYNEFLKQNILIDIKLPKKAKKI
                     PIVLTPKEIKDLVENTSNNRNKLIIEFMFSTGVRVSECVNMKISDLNFEEFTGNVIAG
                     KGNKDRIIILSKNWVAEYKKYLEEREKKVKSEYLFCSDSGKPLSVDTIQKFLKISANK
                     AGITKKISPHKLRHSFATSLLENDVNIRYIQQLLGHANLNTTQIYTKVNTNKLKEIEN
                     PLDHMKK"
     gene            6177..6542
                     /locus_tag="PLK55_01865"
     CDS             6177..6542
                     /locus_tag="PLK55_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOZ35710.1"
                     /translation="MTMRISKLFGMDLYETNATYRGKVYDLVVNLESGIVETITTQPL
                     KVSSKKEAKKIMTEHSIPYKKVISVDDIILINSGNVPVAEREVVREPVAEEQDNSYDS
                     KIRKKYIGYKSKYSTAGRR"
     gene            6891..7487
                     /locus_tag="PLK55_01870"
     CDS             6891..7487
                     /locus_tag="PLK55_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35711.1"
                     /translation="MFRSIYGYTQNVTKKDKKTYWYHREGVLENIPYIRPGKNSIIVP
                     INTEHKIINFFNTGKSPTHNFKEKGEWSINYNIDKIEINQEDIIKTIDAFINSLIVIS
                     IDGKNTKIFDELNNIIIDKEYASKFKKANKDNLISKINNILEIDWIFKTKESSEKVKL
                     LISLFNKAKESFNYSSSSNYTNKDSLTSESLLKTTDES"
     gene            complement(7491..8027)
                     /locus_tag="PLK55_01875"
     CDS             complement(7491..8027)
                     /locus_tag="PLK55_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35712.1"
                     /translation="MGNIFQKLLTKLGLLKKNKNNKKDSNYQETFASNNNNYSNYSNN
                     QSNEKISNTNSMQTQQLELKNLNIKTVDNEKEINHTKIYDVSKISSKKINTIWDVIKA
                     RENTQAEHIIACLPDDEWIDMEDLKNRIKLQYNVEYQNEKSLYPYIKTLTDINLIKVN
                     NSGKKRTWKKNIIIIEKE"
     assembly_gap    8426..8525
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8543..8617)
                     /locus_tag="PLK55_01880"
     tRNA            complement(8543..8617)
                     /locus_tag="PLK55_01880"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8580..8582),aa:Met,seq:cat)
     gene            complement(8732..9310)
                     /locus_tag="PLK55_01885"
     CDS             complement(8732..9310)
                     /locus_tag="PLK55_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35713.1"
                     /translation="MTVNKLKKEILMDKTTTQFLEQIAGPLAPEILKMYNADKTFSPE
                     EISEKLKKKITTIRSTLNSLHYRGIACYKKVRNQQNLFDFYWEIKFKKIIELVMESEI
                     DNLKKIEETLNQQEGRDYFYCPKKCIEAPFEVAAAYNFKCPNCNKPLEMMDSKNLNSA
                     LKKKKNAISKNIEKLRGIVDKIDDKTTGYICD"
     gene            complement(9386..9931)
                     /locus_tag="PLK55_01890"
     CDS             complement(9386..9931)
                     /locus_tag="PLK55_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HOZ35714.1"
                     /translation="MTQNVYKLNILRLDHRPYRDKRITTHCALVGRAFGADEFYFSGT
                     EDSGIITVITKTNENWGGNYRIEFVEDALSYAKNFKENGGVIIHLTMYGLRVQDKIDE
                     IKKLKRTKDLLLIVGGAKVPTEYFQLANYNIAIGNQPHSEVSALALVLYLLDASCLSK
                     EFNNSKLKIMPDNNFKKIIRK"
     gene            9994..10067
                     /locus_tag="PLK55_01895"
     tRNA            9994..10067
                     /locus_tag="PLK55_01895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10028..10030,aa:Lys,seq:ttt)
     gene            10079..10152
                     /locus_tag="PLK55_01900"
     tRNA            10079..10152
                     /locus_tag="PLK55_01900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10113..10115,aa:Lys,seq:ctt)
     gene            10168..10241
                     /locus_tag="PLK55_01905"
     tRNA            10168..10241
                     /locus_tag="PLK55_01905"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10202..10204,aa:Phe,seq:gaa)
     gene            10292..12679
                     /gene="mutS"
                     /locus_tag="PLK55_01910"
     CDS             10292..12679
                     /gene="mutS"
                     /locus_tag="PLK55_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOZ35715.1"
                     /translation="MMKQYLETKKVFPDGILLFRAGDFYEMFYDDAKIASSILNITLT
                     KRGKGETEAPLAGIPYHALDPYVSKLIKAGKKVVICEQIENPKLAKGLVKRAVTRIIT
                     PSTIFSETYDNNYLCSIARLKDNYGFSLIDITTGEFKANNFESFNEILNELSLIKPKE
                     LIITKNILNEKEQINLLKKTIPNVIINYTDNLDYDLSKQKLQEHFGLLFSSFGINKEE
                     VLISCFNAIKYLKDTQFMELSYINNIKEYNLTKYIKIDRKTANNLEIVSNIKDGTTKN
                     TLFDIINNTKTALGYRKLYFDLLHPLTDKSEISKINEAVLVIKENQFLQEELSQNLSF
                     IGDLEKIISKIGFGNCNPRDLTGLKQSLQVIPSIRTTLSETASQKLHELREKLQSLDK
                     VVDLIDSAIADFPPISIREGGFIKPSYSKELYDIVNINKSTHTWLSEYEADLKQKTSL
                     KSLKIKFNKIFGYYIEIPKSQTNGIPEYFERKQTLVNCERYTTIELSEKESLVMHAKV
                     RREELEYDLFFEVCKAILKYQEPIREIAKQIAEIDEIYSFAYVSNKNNYNKPELNNSD
                     QLEIKDLRHPLIEQQTAFVPNDCILNSKNKTMIITGPNMAGKSSYMRSVCLALIIGHT
                     GCFVPASIIRLGKIDAIFTRVGASDDITHGQSTFLVEMAEVAYILNNVTKDSLVILDE
                     LGRGTSTYDGISIAWSVVEYLNEKIECKTLIATHYHILNKMSEIYSCINNYHVTAKEE
                     GDSIHFYHKLQKGGINKSYGIQVAKLAGINKEIIDNALKIQRDLENDSFLKNLEA"
     gene            12683..14407
                     /gene="mutL"
                     /locus_tag="PLK55_01915"
     CDS             12683..14407
                     /gene="mutL"
                     /locus_tag="PLK55_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOZ35716.1"
                     /translation="MIKKLSQETINLISAGEVIESPSDILKELLENAIDASATEIIIK
                     IKNSGIDLLEIKDNGLGISKEDLEICTQRHTTSKLDNINDLYNLDTFGFRGEALASIN
                     AISLLEIISADNLNGKGSVFKGGKTVSAACPKGTTIKVENIFYNVPVRKKFLKSKAQE
                     FSKVYNTFLEFVIQYPEIRFHFTSEKKEEVFTSTTQNKRYEQVFGKDILTKVKTIDTK
                     NEFLKIKGIITKPNNYFFYPNNYLYINKRAVYSTQISKLIQKCYKDYLMVQQKPFFVL
                     FIELDPKTLDVNVHPKKRLVKIQNEFIFNLKLKEIIENALFPKGAPEQKSADAPRNLH
                     YFLDTKEVQESADTLKSKIPEIIYDLHENKTQATISVPESQTKKAHLSNYEIREIIGQ
                     LFDTFIVCKTQEGLLLIDQHAAEERINLEKNRHLYESEFKVQSLMIPKSLDFISESQK
                     EFINKKKSLLVSLGFTIEPKGNGFVLTTVPEFLEHYFDHIFFLEMLNEIEETNNFDIS
                     RIKEKILKLKSCKESIKANEQLSISQIYQLIERLDKCKDKSICAHGRPTYLLLNRKEL
                     EKMFKRIT"
     gene            complement(14404..15576)
                     /locus_tag="PLK55_01920"
     CDS             complement(14404..15576)
                     /locus_tag="PLK55_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35717.1"
                     /translation="MLKKILILIILMIAIIAGVVRADVLEERSFASLNYDNFSVQRDQ
                     ELCNYYNFDKMEQTENSDLYLNLIIENYIPVKENISISVYFNGTFENKIENAKILKEN
                     RIKLAGKIQEKNSLKICITNNGLPIVIVSGNSTVGSYIIGEIKPEEFYIKVLASQTHS
                     NTLLPIEIYAENNSPKGVEVTINYADENFLRISNLETVSGETSYSGIIYPGETKILKY
                     YLKTNRNISFGTPQATLTYTNDFGEEKTILADQKVIQMTEKLDKIEVYVDIARNVNVN
                     EEQDGKIIIRNVSEDELKNLTIETSFDNKVILSERQISALKKYEVKEIPFKTTIDSTG
                     KHVFNATVYYNIGDVENGISAEKTNITASVKTDNIKEIIGVFLFITIIVYIWIVRF"
     gene            complement(15657..19178)
                     /locus_tag="PLK55_01925"
     CDS             complement(15657..19178)
                     /locus_tag="PLK55_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35718.1"
                     /translation="MRWIVLFSILLLVLLSGCLDVITTRQKQLCLSSTDVSKTSIPDC
                     NGFSACYKKINNSGFFVLNEVSFETKNKILTYKNNIASSVYYFNKAEKEIQKIYAYCS
                     GENDLKIIRSINDLMFYISKIFNYQDLAWQKSIEILKDYAIYLKSQGVEEITEEEIYV
                     SFALINQNINELRDESINDKIYVGVLKKEAFAARELASNFGFLKSYMTSVNYVDIYAY
                     YSEYIDNPEQELKMPVISKSSNYVFSKLSTFENFQRINTNLKRTDNYNLYILFDKHIG
                     TEDSLFKRFIILNNKINEDLNLALNRINGLEERLENNLFILDKSKLEEYKKYKQNYAS
                     GDMGFGYYLSYLKDLNLEIEKQIELAEEKTKTETEEIKDCEIVIDSIKDYNNLYFKEL
                     ILKFENETNNVLKIEICEKLKNTLVQKDCFLSLEKLALAGAIIDINITTQEECTDLIN
                     QFNYSAGRAEEIELFWAIYSDSSNILEELKRFNLNFEMQIEIAKLKNKLEKYKNKENY
                     NIFLEIKEYLKEINTLNAEIKALFLEATSEQIIKSKEIYYEGGVYFLKLVNSSNIVLT
                     EFCLDASLLELEGAFPLTKNLKLNQETACFDKVYSGTNLFEIGYENKKSITTRIIKLD
                     IDKTLFETIIKNTVSGLKDTLFLGQAAVVDSATYVLNEEGAIEYSTQKENKILYYLHI
                     FQKTDLDTNIGQVENGNLVLLERYDLKNTHSEKVCGNLSFTECKSGLAKLVINGTKIE
                     IDYYANAGQISAPTCFETLETKRVDVYCIMEYAELLEKIKDLLLKINALMNSEFEDIS
                     KESVSAFKAIYEKCNLKESFSYDDIISILKQEPKIIELEKSQNQKFEYLENTKRLLAD
                     ISDSNIEGVYSVEIMKIEKMFFTDPKTAYYLAKKLYEDLANSKNVLVELEEDNYENKL
                     NNLKNLAKSYDIMDLELQDKFLELESTPNNAAAITELENKINETLAKKTETNVEWFTY
                     FKSFEKSDFLEKLQELEWYYSDIDLKDLYSVKYYPAVTVDDAIRIKKKLAFLETVKYK
                     EEATNAVYEYESKRNEDALNIINITTIERLMDLNKEIELVNTGIIQIKKDSNSALNDV
                     LNSRVLSKNKEAIDNIKKDYENRNYLKVIFDSRVLLTTQPKNNNPKIGQILSFGLGGA
                     ILLVFLFNKKPKKLSKDEKKQKILRHY"
     gene            complement(19278..19628)
                     /locus_tag="PLK55_01930"
     CDS             complement(19278..19628)
                     /locus_tag="PLK55_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35719.1"
                     /translation="MKAFLGILSTGKGTWGQVAHIIDNVDYDKVYLISNEWAQDKFNC
                     TKPINWIIIDPRKGFSEIMADLEKQIPTDTKELDLNIHSGSGKEHSVVLALLLQKKIK
                     FQIITINAQGIVKY"
     gene            complement(19646..19966)
                     /locus_tag="PLK55_01935"
     CDS             complement(19646..19966)
                     /locus_tag="PLK55_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35720.1"
                     /translation="MSDNKDNLIKMFSEDPKLLDIVNSKEHMAILTILEQNCADFLNL
                     KKDVFKYIYIKKDIILYNILESLIQKDLIKKIGVNENKVYYITEKGKKFMSLYKETKK
                     EFDI"
     gene            complement(19981..21516)
                     /locus_tag="PLK55_01940"
     CDS             complement(19981..21516)
                     /locus_tag="PLK55_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOZ35721.1"
                     /translation="MDELYKKRISSYFKKCASSKFTKQQFEAKKVSFAKKYGLKKIPL
                     TSDVFLALGIDSPPEFLITKPGRTQSGVTIVAIMTKPGECPGKCTYCPTWKNAPKSYT
                     GFEPASLRAKRNNFDPRAQIKDRLKQLKSTGHPINKIELIVMGGTFPATSKKYQDGFV
                     KSIFQEITNSKDTEKDLNKLKKKAMGSKNRIIGITFETRPDFCDKKTIKRLLDYGGTR
                     VELGAQIIDDNVLKFVNRGHGVKEIIKATKELKDAGFKVLYHVMLGLPGSDYKKDLKS
                     VKEIFTNPDFKPDMIKIYPCLVIAGTKLEQMYYSGEYKALELEKTIKLIADIKELVPR
                     WVRIMRIQRDIPATKIIAGIKKSNLRQLVEEELKKRQKACNCIRCKEPKGQLEDTKGY
                     KIKKEEYIASDGKEIFLYAEKDKYLLGFIRLRYPNKPYIKQITKKTAIIRELHVYGQT
                     TAFEDKNIQHRGIGKKLLKESENLAKKEGYNKIAIISGVGVREYYKKQGYSLSEPYMI
                     KNI"
     gene            complement(21552..22406)
                     /locus_tag="PLK55_01945"
     CDS             complement(21552..22406)
                     /locus_tag="PLK55_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35722.1"
                     /translation="MFNKKIFFIISLLFILPSIHAIQMLEPIMTDINPNKAIELGTVA
                     PGEYFLVSFFLGVDEKYDLITTDNYGSNYLFFENTQKTKESIYTIVKIKENVSGEQQV
                     KIILKNTETGKTESAYIKMTVATNVISSFVLPYDKETKFGVRKDITIKVINKSITTKK
                     ITISSNLPITWFDSKDAEKIKKEVTVVLQPSDSIDVNYSFIPKSIGDKEIQLFVYTDA
                     VPQNSIIQTTTFFTKALFERNEETINLFIKKDLAALYAANLYNFPTFALNAIPAYFFN
                     NIIRIASE"
     gene            22512..23135
                     /locus_tag="PLK55_01950"
     CDS             22512..23135
                     /locus_tag="PLK55_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35723.1"
                     /translation="MNKTLVDSRFAMLKRHHTQTLKDAISKDQVDALVIPFLLKVTEI
                     PDIYTSSSCSGRLLLLGGDKDENKKVSGFVNRYHRQVTFEEIKRDVDNFNTGYLWLKV
                     EPFIFHFATKDYDKAKDILDFCRAQGLKKAGIISAKEGRFTCEVTHTVFMSTLIKIDK
                     KQLISDEYLKLIVDIANKKLAANFKKLEDFENAFLKAFPSKLKIKKK"
     gene            complement(23136..24089)
                     /locus_tag="PLK55_01955"
     CDS             complement(23136..24089)
                     /locus_tag="PLK55_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOZ35724.1"
                     /translation="MEEQKLKNIFFGTVIFGFLLLGSIFLIIDESIKQIILIVLIFIA
                     FIFLLFSANIILQLKDYERAVIFRFGKVNRVGGPGWTLVLPFIEKYSYVTLRTQVVDV
                     PIQQIITKDNIKVTIDAILFLSIENNKESIINSIVKVEDYKKAASYFVIAMLRDILGE
                     FVLTDVISNTENINKKITEEIKGVAKEWGIRVNSVEIRDLQIPEEIVESMHKQKAAVQ
                     KKLAIFEEAESEKAKINAIREATESLSDKTILYYYIKALEKVGEGQSSKIIFPMELTS
                     LLQNISEKINSGNPRINAKEKKELAEYLPLIQSYLEKKKRK"
     gene            24222..25505
                     /locus_tag="PLK55_01960"
     CDS             24222..25505
                     /locus_tag="PLK55_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HOZ35725.1"
                     /translation="MVKETTKKAKPEEVVIVEQKVQPAQEHILILTVDRDNDIGRKIG
                     VSGPIVGYESNLKMATDLLLKDPEESDANAVFAILKKYSELKKDYVVEVATITGHSKE
                     NLFFADKNIALQLKQVLEDFPASAFVLITDGAEDDQVVPIVQNFGPIISKEVVIVRQS
                     QAIESMYYTIKKAIKDPAFSRIIFGIPAIVLLLFFFFKSYAFQIIALVLGVFFLIKGF
                     HLESKISNFFNSIFSKFSITKISFPFYVASVFFLIYAGITGVNLFLANDSFILWQRII
                     YVLRAILLYLVFCIGSYVVGNIVDLFYTRALYKLGHAIFAIISTFVFLGLLDLSLQFV
                     LGETSMRMLITVILISSVFLFILYKITSVFDITKDITELLVGLPVISKYGVWLGEVIA
                     VDKTKQTISFKSRNGKVITVLSKKHFFIQNGQVII"
     gene            25645..25923
                     /locus_tag="PLK55_01965"
     CDS             25645..25923
                     /locus_tag="PLK55_01965"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOZ35726.1"
                     /translation="MYKYAIARQLATKKLITNRGDNIGRLVDLMVNEVSGEIEALLVE
                     IDPSSKVVKQLDVQGNLLEIPYRSVIAVSDVFVIDEKTLLTKDEGTFN"
     gene            25987..26553
                     /locus_tag="PLK55_01970"
     CDS             25987..26553
                     /locus_tag="PLK55_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35727.1"
                     /translation="MFADLKNKKAISPIITTILLVVVGVILITVVLNFGKNFTTKSLD
                     QTTNFGSLKVSDATNFVYPKYAKDGVLQINYAPPVKLENESITITQYQLLDVLDSEVV
                     TLQTPVTLNNNSLSTIQLDCLYEYALSKPDFTIQLITAQNTYIEVKLRDPNMVCSPGG
                     TGTIEDPIIVCNAEDLNAMRLDLMQITL"
     gene            26568..27524
                     /locus_tag="PLK55_01975"
     CDS             26568..27524
                     /locus_tag="PLK55_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOZ35728.1"
                     /translation="MRCFSRQRADGWLPIGDYEDLFSGSFNGNNHKISNLYISDSDGV
                     DLGLFGYFDAGEISNLGLEDLNISGKLYLGGLVGEMDSGEITNCYTTGNIYELSGGGR
                     IGGIVGGNWGGYITNSYSETNIYAGSGDFYYGGLIGNNNGDVENCYATGFVSGRSYVG
                     GLIGANFGGSITNSYATGDVNVLNTYADAGGGFIGDNEGTISNSYSFGDVTRVTGSNT
                     TFGGFTGYNGGTITKSYSTGRVIYAGTTNPTNRGFSFDSVSNNNFWDTQTSLQATSGG
                     GALGKTTAEMKTPSTYTDWDFITVWAIHPSKNNGYPYLRWQN"
     gene            complement(27537..28613)
                     /locus_tag="PLK55_01980"
     CDS             complement(27537..28613)
                     /locus_tag="PLK55_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35729.1"
                     /translation="MKKLEVTSTAFFYIVKELQAEVVGGYINNLQLVNHEDNNLIKIK
                     IHKTKTKELIVSPNILFLTELAYPVNPESGGLIKFLKKKLYNQKIQEIKQDKNNRVVY
                     FKLDDYYLIFEFFSNSNIILTDLELKIITSKQKEEWKDRIIKKNEKYSFPQGKEITEK
                     TQKELLEDIKDKNLKATISYFVKEYNIYSGYINQEKTQKEIVEKTKELYDLRRPSLAL
                     EKGKNEDESLVLVAEGKEKQNSFGFFKIISEYFAEKLKTEETKIEKKSKNKQTSILDS
                     QIKTRKEYEKIASNLQIEGEKIYEYFQIIEKVNEQIRLAREKKIAPEEIIKKLNDYFS
                     KNQKELSIKSIDTKERSYVLEIKE"
     gene            complement(28621..29679)
                     /locus_tag="PLK55_01985"
     CDS             complement(28621..29679)
                     /locus_tag="PLK55_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35730.1"
                     /translation="MDVELIQMIMITCIAIVFGVAFTFINFRKKITVPVTKEQNSWQR
                     IEALNTQKKELLKRKEELSYKYSAKSISDDVYSNNLKNITTELAKIDNEINGEVSKLT
                     ELQKTQDTGSDLRFQNIKLKGELNETKLERDNLKNRIKELEDFIKNLSGTKNLSTSIK
                     DNLQNKYYEQILDKYKELINEQERKTISQIKATVNPNDLTIKSVILKHKPIGYEYNKD
                     YIETLRKIYSFLKTEIEVVKTDIKILYWLDATCILKNKLADEQDISAFLCSIMQGLND
                     QEAMVYVILLEDDKTHAFVKTRYKNQFYIFDLTQKGPFEMFTNTDEKKLLEDYKFNGL
                     RIKKIIYRYNQNNYIDAE"
     gene            29938..31194
                     /gene="tuf"
                     /locus_tag="PLK55_01990"
     CDS             29938..31194
                     /gene="tuf"
                     /locus_tag="PLK55_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HOZ35731.1"
                     /translation="MASPKPKINVVFIGHIDHGKSTTVGRLMYDTKNLSEQDYRKLQA
                     MAEEKGKGTFAFAYMMDITKEERERGITIDVNYKKLESDKYSFTVIDAPGHQDYVKNM
                     ITGTAQSDAAVLVVASKDGVMSQTKEHAFLAQVMGIKQVIVAVNKMDDVNYGEEGFNK
                     VKADIQALFKGKFKDDQYVVIPISAWNGENITKASDKMPWFKGPSLFEAMNNFKAPEV
                     LTGKPLRLPIQDVYSIKGVGMVPVGRVETGILRPNDKVIIMPEGIATQIKSVEAHHEQ
                     LPEALPGDNIGFNIKNVDKGAIKRGSVVGPENSPPTVAEEFTAQIIVLNHPTAITAGY
                     TPVFHIHTAQMSCAIVELIKKVAPTVEDAPKFLKTGDAAVVRIKPLQPLACETYKDFP
                     QLGRFAIRDMGKTVAAGVIQEIKKRA"
     gene            31238..31546
                     /gene="rpsJ"
                     /locus_tag="PLK55_01995"
     CDS             31238..31546
                     /gene="rpsJ"
                     /locus_tag="PLK55_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOZ35732.1"
                     /translation="MQTARVIVTGTDYKKVTEFCKEIIDVASKSGVKHTGIIPLKTKK
                     LVVPVRRGVSAGGTETYEKYQLRIHKRIIDIKADDKILRRIMRVDVPADLQIGIELKN
                     "
     gene            31631..33001
                     /locus_tag="PLK55_02000"
     CDS             31631..33001
                     /locus_tag="PLK55_02000"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOZ35733.1"
                     /translation="MFKQTKKNSFVDESNKVKIIYSKSLFSKLDKSCIEQLYSISKLP
                     GICDYVYGFPDLHVGHGAPVGSCFVVNADNDPIISPKAIGTDINCGIRLIRTNLKSKD
                     LSEKQYKEIVKAFEKLPLGLSKDGVKITKQDLTDICYQGLEWAVKNSYADKFDKLYVY
                     RQGCFRDASPKYLSKAAIEKGLTQLGTLGQGNHFIDLLVVDKVFDKKFCKRHKIEKDQ
                     ILIVLHTGSRGLGYQVAEEFTKICKHKDPIEYLYLNSKDGQHYYKTMCAAANYAFVNR
                     AVLSKKVVDALEKTLNLGRGDINARLLYDCSHNFAKLETHNKKRYLVTRKGAVRGIIS
                     ERLSDNSEFLETGTPVILPGSMLDDTFVILPKEKIRTETFSTLPHGCGRVMSREKAKE
                     KISASDLKQKLLKKGIYLSGHSENVLREEQPEAYKSSKEVVDSMVDARLCKKAFSLRP
                     KVVITG"
     gene            33020..34033
                     /locus_tag="PLK55_02005"
     CDS             33020..34033
                     /locus_tag="PLK55_02005"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOZ35734.1"
                     /translation="MHDIKLLSCRSGEHFANNVLKYLNELLLENNEPEITLTPIQEIR
                     FANTEIKSNILESIRGSNVFIIQDVSNSIYAMSVDDNLRALTTLIDAARVASAKEITV
                     IVPVFPYARQDKIWGRECLTARLVSREMELAGARTILSLDIHNPAIAGFFKYAILENL
                     YARKAIKKYILDNVSLNNLLISSPDVGGMKRATVLASKLGVGAITIYKERDYSKINQV
                     ETMEVIGKVKDKDVFLIDDILDTGGTVYNAVKSLKEKGANKVYFACSLCLLNGSAVER
                     MDELYDNGLLEKIIATDAVYIEKDFIEKHPWLEVIPISDYFAKAIFNIHFKKSINQLF
                     DKD"
     gene            34110..34292
                     /locus_tag="PLK55_02010"
     CDS             34110..34292
                     /locus_tag="PLK55_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35735.1"
                     /translation="MENEIKCHFCGGTAIIKYRNVVLDDGKITIKYESYYEFKKCKEQ
                     FCTSEQMFTLDKRLHE"
     gene            34502..34819
                     /locus_tag="PLK55_02015"
     CDS             34502..34819
                     /locus_tag="PLK55_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35736.1"
                     /translation="MESTAILIVLIINSVLLILLFVFMIRFVVNKFKEQITDTNERLK
                     DIEAKYNKLDIQFSRISALHVSQQKNDISKDIEKVKLVVQGIDKKTEKIYKRTEKMYG
                     KKQ"
     gene            34881..35393
                     /locus_tag="PLK55_02020"
     CDS             34881..35393
                     /locus_tag="PLK55_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35737.1"
                     /translation="MGILRRTTTIKKPAVKRTVKPKATRIKGTIKISEPTRVLLQKKN
                     HVFILESKFKPLAKEYPESKKYVALISNVYLNAEKEILALRERAKGHHNISPLIDLLE
                     GRYKQVSKNLINFINEPKPNIFRFMEVEMQASNYLKEHKQIEEILSPKPTIRGSGHPG
                     KGDYNPRRRS"
     gene            complement(35390..>35734)
                     /locus_tag="PLK55_02025"
     CDS             complement(35390..>35734)
                     /locus_tag="PLK55_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOZ35738.1"
                     /translation="PEYKYLIPLAQIIQMKIGKGELYNKRVLEKYKKLITKYTEIEIM
                     LELPEEKLKEIDEDVAKYVIAFRKGLVVFRPGGGGHYGVPFICFSEEEKKAKEIEILK
                     EQEIPFVQKTLF"
BASE COUNT        12028 a         5283 c         5240 g        13083 t
ORIGIN      
        1 aaattataaa ctcattaatc cttttttgtt ttcagtatta aaatctttat ctgcgccatt
       61 tagcttttct ggaataaaaa ctgaatttcc aatcttgtgt gcttctatga ttgtgttttt
      121 aatttcttga atattagtat tatctgtaaa ataaatcttt atatcaacaa cgccgtctgt
      181 gtttttctca aaaatcgatt gtaatcttat aaagaactgt ggagagacgt cttttgcaag
      241 aggaaaattt aattttatgt cttttggaat agtggattct atctcttgtt ttgtccaggt
      301 agcctttaga tcagaaacat ttactaaaag ctccttgttt attttttcaa acttgtggcc
      361 attaatccaa attattggct tgtaattaat tattgcatca acacctaaac tgcactcgtt
      421 agcaatagaa tagaaatcag aaaaaattat tgaaaacgta gacgaatttc ttaatccgta
      481 ttttttaata ttatttctaa gagcaatcca atcgattttt ggcatatttt ttattagctt
      541 tttagatttt gttaaatctg tagaaaataa ggtcttttga actggcgctt tttcaaaaga
      601 aaaattagat aactcgtatt tgctttttat aaactttaga aatgggcctc tttcttttgc
      661 aagttctgtt gattttaatt tagaataata attaatatag tcagataaag aatatgcaaa
      721 attaattgca tcatcactgt tatatgggat atctaacttt gttaagaggg tataaaaacc
      781 agtaattgaa agaaaaatgg atctggtttc tttaagctct tgtaagtatt tttcatttat
      841 tgtttttgaa acatcaattg tattgtctaa aaattcaata gtatcccaaa taacgttttt
      901 taattttagc caatcaaagg tctttgtatt gcctaaaaaa ttaacaaatt tagaaacatc
      961 aataaccccg ctaacaaaag acgcattttc aaaaaccggt tgcataccaa ctggcgaaat
     1021 tgtgaatttt ttatagtctt ctaaaaatgg gtttttctta ttaattgtat caacaaaaat
     1081 aaggcttatt ttttctccag taattatctt agacgaaatc aaatcaagta gtgctaatga
     1141 agaaatcttt tgcttctttt cagaatttat atctattgtg taatctgtgt tttctgaaac
     1201 tgcagtcata aacctgtcag ggattaatat tgcaaaatta tcctcgtttt gcgttgctga
     1261 atagtttaag aacgaaacaa tgtcagggtg ttctatagat atatagtata aattattaga
     1321 agaattaata ttataaccaa ccattgactt tgcagaaaca aacaaatcaa taattcttat
     1381 tggtccacat gcgtttctat tgatagactt tactttctct ttttctgagc gtattttact
     1441 taagtcaata gaaatagaag agccgtattt ctgaacaatg cttgattgag aaagagtatc
     1501 aaaaatactc tcaattgtgt catcaatttg aaaagaataa ccagaaagca cttgctgatt
     1561 taaagtgata tctcgcaaaa atgtagaact tggatagaaa acgccttctc taatatattc
     1621 aaaaatcttt ttgtttgttt tttcgtgttg attgttttgt aacttattta ggtgggcttg
     1681 aatatttagt aattttgttg gcaggctatc tgtttcttga atatttaaaa ttagttttaa
     1741 aattagttct tttatttcgc tatcagaagg aattgtatct ggcgttgctt ttttaaaaat
     1801 ataagcctgc aaaagatctg cttgattctc ttgatgaaga atttctttta ttatttcgtc
     1861 aagcttttca aaagggattt cttttgaata ctttgcattt actttatcaa atattaaaaa
     1921 tgctaacttc tctgctaaat ttaaattagg atccccatag ttacacatgg tctcaaataa
     1981 cattattgca attttttctt tagaaaactc ctctgtttct ccattctttt gtatcactgt
     2041 gttaacaaaa accatatttt ttcactttta tcttaatata taatctttag agtcctttaa
     2101 gagttgagaa tatattgttt tgtaatcttt atattctttt attgtgttgg tattcatgaa
     2161 atcttggaca aatatttttt gccctctaga aaaatcaatt attttattaa aactttctat
     2221 acttagctct ggatgcgtgg ttattctaaa ttcgtgtgga atatttgaat ctttgattag
     2281 ctcaaatgaa agttttatat tttctgttgg aacgtttgta tatttattat aattttcaaa
     2341 aacgtctttt aggtccattg caatataatc tacaagatta ttctcaattg ttttttttaa
     2401 catctctgga ttagagccat tagagtctag ttttattaga tagcctctat cttttatttg
     2461 ctttataaat tttataagat catcaccaaa aattgttggc tcgccgcccg ttataactac
     2521 tgcatctatt aggccttttc gcgcgtccaa gaaatcctgt atttcttttt cagagaggtc
     2581 tggttttgtg tctaatagct caggcagcac cagttgttta ttataacaaa aaccacaaaa
     2641 aaaattacag ccttttagaa aaattacaca agatattttg tcaggatagt ctattgtact
     2701 aaatttttct atagccccta ttcttaaaga caatattttt cacatttatt ttttttcagg
     2761 aacagaaaac aattttctat cttgaaattc tgattgttta ccatagttcc atgcgtctac
     2821 tcttctaaca tatcctacga ctctagaata tatttcgcat ttctgtcttt tagactctaa
     2881 ttcactactc attttatttc ctccttatat cctatctctt catcacaaat tggacaaaac
     2941 tcgtgttctc caaaaatata tccatgtttt gggcaaactg aaaaagttgg tgtaaatgta
     3001 aaatatggca atttgaatct tgttgtaatc ttttgaatta acaattttat tgcttctctt
     3061 gatggtttct tttcgccaat aaaaatgtgg atcacagttc ctcctgtgta tttacattgt
     3121 aattcatctt gcaattctaa aacctcaaaa atatcgctag agtaatttac aggtaattga
     3181 gaggagtttg tataatatgg ttgtgcgcca ttattaatat aattttcttc gtttgcaaca
     3241 attattgatg ggtatttttc tttatcatag cttgcaaatc tatatgttgt gctttctcct
     3301 ggtgttgctt ctagattata aagatttcct gtttcttctt gatattttaa aattctatct
     3361 cgcataaagt ccattacttt aattgcaaat gcttgccctt tttctgtata tatcccttct
     3421 cctaaaaaat taacagcaga ttcattcaag ccaagtaatc caattgtaga aaagtgattt
     3481 ttccaaaaac tatcaaatct atcttttata gctttaagat taaatttaga ataaggatat
     3541 aatccgtcat cggtatattt ttccacaatt ttccttttta tatcaagtga tttttttgca
     3601 agatccatta aatgtgctaa tttttcaaaa tattcttctt ctgatttaga gagataacct
     3661 aattgtgcaa gattgatagt tacaacgcct attgaaccag tcaaaggatt agaaccaaat
     3721 aatccgccgc ctcttttttg taattctctg ttatcaagtc ttaatctaca gcacatgctt
     3781 cttgcgtctt ctggtttcat atcagaatta acgaaatttg caaagtatgg tgtgccgtat
     3841 tttattgtta agtcaattat cttatttgca agttctgttg accaatcaaa gtcttttgta
     3901 acattaattg ttggtattgg aaaagaaaac atatttccta tcgcatctcc ttcatacaac
     3961 acatcaagta aagccatatt aaaaatatcc atttcttttt gaaaatctcc atatgtttta
     4021 tcttgtgatt ttcctccaat aataacagcc tgatctttaa aggtggaggg gactgttaaa
     4081 tcaagagtta tattcgaaaa tggagtttga aaacctgttc ttgttggaac attcatatta
     4141 taaataaatt tttctagaga tgctcttgtt tgagcataat caagattgtc atatcttata
     4201 aatggtgcaa gaagagtatc aaaattacta aatgcctgtg caccagctga ttctccttga
     4261 agagtatata taaaattaac aatttgtcct aatgcagtat ctagatgttt tggcggagat
     4321 gccacaactt ttcctgggga ccctctaaat cctgtttgta atactgattg gaggtcccag
     4381 ccaacgcaat aggaccctaa agtattaaga tcatgaatat gaagttgcct tttttcatgt
     4441 gcttctttaa tttcaggagg gtatattttt ttaatccaat agtcagaaat tatttttgtg
     4501 gataaataat tatttagccc ttgaagcgag taatccatat tactgttttc tttaacttgc
     4561 caatctgaaa tattaatata gctttcaaca gcatttgtgc tatctattaa tttagaaagg
     4621 tttctaaaat cgtcgtgttg ccttctataa attacatagg ctttaactaa ctttgtaaat
     4681 ccttctttta ttaatatgtc ttctatagtg tcttgaatat catttatttt tggtaatgtt
     4741 tttttattaa agtattcttc tgttagtttt atatctactt tttttgttaa ataatctgct
     4801 aagtcaatat ctggattatc gattgcatca agagccttaa gaatagctaa tttaatttta
     4861 taaatattat atggtatctt ttcttctttt ctattgatta tttgtttggc agccattcat
     4921 atctcacatt tttatttaga attaaaaata gtatatttat agtagttcat caagtttata
     4981 aaggctatcg gtttgtctga gaaaccagta tttatatgtg tttttggttt atttgtgata
     5041 gctggcttta tataacaaac tattggcttt gaaatattta gttaaacaaa ttaagtgctt
     5101 cggcataatc tattttataa gcatttccgt tctaattgtt tagataaaat atggatgaaa
     5161 agaacataga aatcttaaaa aaattcgaaa atgaattaaa tattattggt tatagtcaaa
     5221 aaactataga gacttatttg atatatctaa ataaattctc tgagtttata aaaaaagatc
     5281 taaaagaagt aaactctgat gatattgtaa attttttatc atatcttaaa actaaaaaaa
     5341 attcaggttc tgcaactatg aatctggtgc tttcagcaat caaacatttt tataatgaat
     5401 ttttaaaaca aaacatttta attgatatta aattaccaaa gaaagcaaag aaaataccaa
     5461 ttgttttaac tccaaaagaa attaaagact tggttgaaaa cactagtaat aatcgtaata
     5521 aactgataat tgagtttatg ttttctactg gtgttagagt ttcagaatgt gtaaatatga
     5581 aaatatctga tcttaatttt gaagaattta ctggaaatgt tattgctgga aaagggaata
     5641 aagacagaat aataatttta tcaaaaaatt gggttgcgga atataaaaaa tatttagaag
     5701 aaagagaaaa aaaagtaaaa tcagaatatc ttttctgttc tgattctggt aagccacttt
     5761 ctgttgatac tattcaaaaa tttttaaaaa tatctgcaaa taaagcagga attacaaaaa
     5821 aaattagtcc ccataaatta agacattctt ttgctacaag tcttcttgaa aatgatgtta
     5881 atattagata tattcaacaa cttttagggc atgctaatct taacactact caaatataca
     5941 ctaaagtcaa tacaaataag ctaaaagaga tagaaaatcc actagatcat atgaaaaaat
     6001 agttttttac cccctcactt cctgtattaa gctgaaatta actttaaatt gcggtttaga
     6061 aagcaaactt taaaacattc tatttttact tttagaaata gtaacatata taaatccttt
     6121 gtataacctt ttagtatact aataaaatat atatttaata tttggaggaa taaaacatga
     6181 caatgagaat ttcaaagcta tttggtatgg atctttatga gactaatgct acatacagag
     6241 gaaaagttta tgatttagtt gtaaacttag aatcaggtat tgttgagaca atcacgactc
     6301 agccattaaa ggttagctct aaaaaagagg ctaaaaagat aatgacagag cacagtattc
     6361 catataagaa agttatatca gttgatgata ttattcttat taactcagga aatgttccag
     6421 tagctgaaag agaagttgtt agagagccag tagctgaaga acaagacaat agctatgatt
     6481 ctaaaataag aaagaagtat attggctaca agagcaaata ctcaacagct ggaagaagat
     6541 aaataattaa tttatctttt attttctatt ttttattgtt taatttaagt cttactataa
     6601 gactaactat ccctttagtt cttgtaatat ttatttaata ggaaagcttt atattatgta
     6661 taattaatca atatacttaa atgtaattaa taaaaaaata ttattgttat tactattatt
     6721 tattgtaata tatatatata tatatttata cataacataa tttataatat aataatactt
     6781 aatacaagaa gtatgggggg gctcacatgg ataaaataat tagtaattat ataaaatata
     6841 aatttatatt taacaatgat ataaaacact cttcttattt atatcaaaaa atgtttagat
     6901 ctatatatgg ttatactcaa aacgttacta aaaaagataa aaaaacatat tggtatcata
     6961 gagaaggagt tttagagaat attccttata ttaggccagg aaaaaatagc atcatagttc
     7021 caattaatac agaacacaaa ataattaatt tttttaatac aggaaaaagt ccaacacata
     7081 attttaaaga aaaaggagaa tggtctataa attataatat tgataaaatc gaaattaatc
     7141 aagaagatat aattaaaaca attgatgcat ttattaattc tctaattgta atttcaatag
     7201 atggtaaaaa tacaaaaata tttgatgaat taaataatat aataattgat aaagaatatg
     7261 cttcaaaatt taagaaagca aataaggata atctaatatc aaagattaat aatattttag
     7321 aaatagattg gatatttaaa acaaaagaat cttctgaaaa agtaaaatta ttaatttcgt
     7381 tatttaataa agcaaaggaa tcatttaatt attcatcaag tagtaattat acaaataaag
     7441 atagtttaac ttctgaatca ctattaaaaa ctactgatga aagctagtaa ttattctttt
     7501 tctattatta taatattctt cttccaggtt ctttttttac cagaattatt aacttttata
     7561 aggtttatgt ctgttaatgt ttttatataa ggataaagac ttttctcgtt ttggtattct
     7621 acattatatt gtaattttat tctgttcttt agatcttcca tatctatcca ttcatcatct
     7681 ggaagacacg caataatatg ttctgcttgt gtattttctc tagctttaat tacatcccaa
     7741 atagtgttaa tttttttact gctaattttt gaaacatcat agatttttgt gtgattaatt
     7801 tccttttcat tatctactgt ttttatattt aaatttttta attcaagttg ctgagtttgc
     7861 atggaatttg tatttgatat tttttcattt gattgattat ttgagtaatt actgtaatta
     7921 ttgttattac ttgcaaaagt ttcttggtaa ttactatctt ttttattatt tttatttttt
     7981 tttaaaagac ctaatttagt caatagtttt tgaaatatat tgcccatatc atattatagt
     8041 aattaccgta attattttaa agggattggt aattacagta attatataac ctcatttttg
     8101 gtcattaccg taattactat atttagcttt ttgttactaa aaaatcagca aattagttat
     8161 gtataattat ctaaaaaaac acctaaaaaa taaaggtttt tactgtaatt accaaaaaat
     8221 ctagcgtaat taccaaaaaa ataagcaatt ttagtgttat ttatataaaa ataaagatta
     8281 tttttagtta ttttttaaaa aataggttaa tttggcccat tattctaaat taaggccatt
     8341 aaatgttttt ttaaataata tttagagatt tatttaagtt gttaatcttg tccaagattt
     8401 agtgattttt agcttaaaaa ttagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnttaaa aattagaaaa tatagcggag gatggatttg aaccatcgac cttcgggtta
     8581 tgagcccgac gggcactcca ggctgcccca ccccgctatt aagcaaaata taaataaaga
     8641 gttttttttg aaggttataa tatatatatt atacaaaagg aatataaata aattctattt
     8701 tgtgaattaa ttaaaaagaa gcgcttttta tttaatcgca gatatagcca gttgttttat
     8761 cgtctatttt atctactatg cctcttaatt tttctatatt tttagatatt gcatttttct
     8821 tcttttttaa tgcactattt aaatttttag aatccatcat ctctagaggc ttattacaat
     8881 tagggcactt gaaattataa gctgctgcaa cttcaaatgg tgcctctata catttcttag
     8941 ggcagtaaaa ataatctctg ccttcctgct gattaagcgt ttcttctatt tttttaagat
     9001 tatctatctc actttccata actaattcga ttattttttt aaattttatt tcccaataga
     9061 aatcaaaaag attttgctga tttcttactt ttttataaca cgcaatgcct ctataatgca
     9121 agctgtttaa agtagaccta attgttgtaa tctttttctt taatttttca gaaatttctt
     9181 caggagaaaa ggttttatct gcattgtaca tttttaaaat ttcaggagct aatgggccag
     9241 caatttgctc tagaaattga gtagtggttt tatccattag aatttctttt ttgagcttgt
     9301 ttactgtcat atgcttttca ccttaaaaac agaataaaat agaattaagt aaatatatta
     9361 ataccaaact aattataaag taatattact ttcttataat ttttttaaaa ttattgtctg
     9421 gcattatctt taatttacta ttattaaatt ctttagataa acaagaagca tccaacaaat
     9481 aaagcactaa ggcaagagcc gaaacctcgc tatggggttg gttgccaatt gcaatattgt
     9541 aatttgctaa ttgaaaatat tcagtaggca cttttgctcc tcctacaatc aaaagcaaat
     9601 ctttagttct ctttagtttt ttaatttcgt ctattttatc ttgaacccta agcccataca
     9661 tcgtaagatg aataattacg cccccatttt ctttaaaatt tttagcataa gacaaggcgt
     9721 cttctacaaa ttctatacgg taattgcccc cccagttctc gttagtcttg gtaattactg
     9781 taattatacc agaatcttct gttccagaaa agtaaaattc atcagcccca aaagccctac
     9841 ccactaatgc gcaatgtgtt gtaatccttt tgtctctgta aggcctgtgg tctaaacgaa
     9901 gtatattaag tttataaaca ttttgagtca taaatatatt atacactaat tgattatata
     9961 tatttcgacc aataagaatt ttagtgaaaa tatggatctg tagctcagct tggtagagca
    10021 cgggactttt aatcccggtg tcgagggttc gaatccctcc aggttcaatt taccacttgg
    10081 gcccgtagct cagcttggta gagcacaaga ctcttaatct tggtgtcgtg ggttcaaatc
    10141 ccatcgggct cacattggta aactttcgag actgtagctc agcctgggag agcaataggc
    10201 tgaagaccta tgtgtcgtgg gtccaaatcc caccagtctc atatttacca attttcaaag
    10261 aaaagaaatc atggatagaa atttaactcc gatgatgaaa caatatctgg aaactaaaaa
    10321 agttttccca gacggaatat tgctatttag ggctggagat ttctatgaaa tgttctatga
    10381 tgatgctaaa attgcgtctt ctattcttaa tattacgcta acaaaaagag gtaaaggtga
    10441 aaccgaagcg ccattagcag gtattccata tcacgctctt gatccgtatg tttctaaact
    10501 aataaaagca ggtaagaagg tcgtaatatg cgaacagata gaaaatccaa aactagcaaa
    10561 aggattagtt aaaagggctg taacaagaat tattacacca agcactattt tttcagaaac
    10621 ttatgataat aattatctct gttccattgc gcgattaaaa gataattacg gcttttcttt
    10681 aatagatata acaacaggag aatttaaagc aaacaacttt gaaagcttta atgaaatttt
    10741 aaatgaattg tctttaatta aaccaaagga gttaattatt acaaaaaata tattaaatga
    10801 gaaagagcaa attaatcttt taaagaaaac aattccaaat gtaattataa attatacaga
    10861 taatctagat tatgatttat caaaacaaaa gctacaagaa cactttggat tattattttc
    10921 atcttttggt ataaacaaag aagaggtttt aatttcttgt tttaatgcaa taaaatatct
    10981 taaagataca cagtttatgg aattgtctta tattaataat ataaaagaat acaatttaac
    11041 aaaatacatt aagatagata gaaaaaccgc aaacaatcta gaaattgttt caaatattaa
    11101 agatgggact acaaagaata ctctttttga tattattaat aacacaaaaa ctgcacttgg
    11161 ttatagaaaa ttatattttg atcttttaca tcctttaaca gataaatcag aaatttcaaa
    11221 aatcaacgaa gcggttcttg taattaaaga aaatcagttt ttacaagaag agctatcaca
    11281 aaacctttct tttataggag acttagaaaa aataattagt aaaattggtt ttggtaattg
    11341 caatcctaga gatttaacag gcttaaaaca aagtttacaa gtaataccaa gcattagaac
    11401 tacactttca gaaactgctt ctcaaaaact tcatgagctt agagaaaaat tacaaagcct
    11461 tgacaaggtt gtagatttaa tagattctgc aatagccgat tttcctccta taagcattag
    11521 agaaggaggt tttatcaagc caagttattc taaagaactt tacgacatag taaacataaa
    11581 taaatcaacc cacacctggc tttcagaata cgaagcagac cttaaacaaa aaacaagcct
    11641 taaatcttta aaaataaaat tcaataaaat atttggatac tatatagaaa tacctaaatc
    11701 tcaaacaaac ggcattcctg aatactttga aagaaagcaa acgcttgtta attgcgagag
    11761 atatactact atagaattat ctgaaaaaga atctttagta atgcatgcaa aagtaagaag
    11821 agaagaatta gagtatgatc ttttctttga agtttgtaag gccatcttaa aatatcaaga
    11881 gccaattagg gaaatcgcaa agcaaatcgc agaaattgac gaaatttatt catttgcata
    11941 cgttagtaat aaaaacaatt ataataaacc agaattaaat aattcagatc agcttgaaat
    12001 aaaagatctg aggcatccat taattgagca acaaaccgca tttgttccaa atgattgtat
    12061 tcttaattcc aaaaataaaa caatgatcat aactggcccg aacatggctg gcaaaagcag
    12121 ttatatgaga agtgtttgtc ttgcactaat tattggacac acaggttgtt ttgttcctgc
    12181 ctctattatt agattaggta aaatagatgc aatctttacg cgtgttggcg catctgatga
    12241 tatcacccat ggccaaagca cttttcttgt agaaatggca gaagttgcgt acattctaaa
    12301 taacgttaca aaagacagcc ttgtaattct tgatgaactt ggtagaggca caagtaccta
    12361 tgatggcata agcattgcgt ggtctgttgt agaatattta aatgaaaaaa tagaatgtaa
    12421 aactcttata gcgacacatt accatatttt aaataagatg tcagagatat attcctgcat
    12481 aaacaattat catgttacag caaaagaaga gggtgatagt attcatttct atcacaaact
    12541 tcaaaaaggc ggaataaaca aaagctacgg catacaagtt gcaaaactgg caggcattaa
    12601 caaagaaata atagataatg ctttaaaaat ccaaagagat ctagaaaacg attcttttct
    12661 taaaaattta gaggcctaat aaatgattaa aaaattaagt caagaaacaa ttaacttaat
    12721 aagtgcagga gaagtgatag aatctccaag cgacatatta aaagaattat tagaaaatgc
    12781 aatcgatgct tcagcaaccg aaataatcat taaaataaaa aattccggaa tagatttact
    12841 tgagattaaa gataatggtt taggtatatc aaaagaagat ttagaaatat gcactcaaag
    12901 gcacactaca agcaagcttg ataatattaa tgatctttat aacctcgata cttttggttt
    12961 tagaggagag gcgttagcta gcataaatgc aatctctcta ttagaaataa tttctgcaga
    13021 caatttaaat ggtaagggca gcgtttttaa aggcggcaag acagtttccg cggcttgtcc
    13081 aaagggtaca accataaagg tagaaaatat tttctataat gtgcctgtaa gaaaaaaatt
    13141 tctaaaatca aaagcacagg aattttcaaa agtctacaat acgtttttag aatttgtaat
    13201 tcaatatcca gagattaggt ttcattttac ttctgaaaaa aaagaagagg tttttaccag
    13261 cactacacaa aacaagagat atgagcaagt ttttggtaaa gatattctaa ctaaagttaa
    13321 aacaatagat acaaaaaatg agtttttaaa aatcaaagga attattacaa agccaaacaa
    13381 ttattttttt tatcccaata attatctata cattaataaa agggcagttt atagtactca
    13441 gattagtaaa ttaattcaga agtgctataa ggattattta atggttcaac aaaaaccatt
    13501 ttttgtttta tttatagaat tagatcctaa aacccttgat gttaatgtgc accctaaaaa
    13561 aaggctagta aagattcaaa acgaatttat atttaatctt aaacttaagg aaattattga
    13621 aaatgcacta tttcccaaag gcgcccctga gcaaaagtcg gcagacgccc ctagaaattt
    13681 acattatttc ttggacacca aagaagtaca agaaagcgcc gatacactaa aatcaaaaat
    13741 tcctgaaata atatatgatt tacacgagaa taagacgcag gctacaatat ctgttccaga
    13801 aagccaaaca aaaaaggcgc atctttcaaa ttacgagatt agagaaataa tcgggcaact
    13861 atttgatact tttattgttt gtaaaactca agaaggcttg cttcttatcg atcagcacgc
    13921 tgcagaagag cgaattaatc ttgaaaaaaa tagacatctc tatgaatcag aatttaaagt
    13981 tcagtcttta atgattccaa agtctctaga ttttatttct gaatcccaaa aggagtttat
    14041 aaataaaaaa aaatctttac ttgtaagcct gggttttaca atagagccta aaggcaacgg
    14101 ctttgttctt acaaccgtcc ctgaatttct agagcattat tttgatcata ttttttttct
    14161 tgagatgcta aatgaaatcg aagaaacaaa caattttgat atatcaagaa ttaaagaaaa
    14221 aattctaaaa ttaaaatctt gtaaggaatc cataaaagca aacgagcaat tatctatctc
    14281 tcaaatatat caacttattg aaagactaga taaatgcaaa gacaaaagca tctgcgccca
    14341 cggcagacca acgtacctat tactaaatag aaaagaatta gaaaaaatgt ttaaacgtat
    14401 tacctaaaat cttacaatcc agatataaac gattatggtt ataaataaga atacgccaat
    14461 tatttctttt atattgtctg tctttacact tgctgtgata tttgtttttt ctgcagaaat
    14521 tccgttttct acatctccta tattgtaata tactgtggca ttaaatacgt gtttgcctgt
    14581 agaatctatg gttgttttaa atggtatttc tttaacttcg tatttcttaa gagcagatat
    14641 ttgtctttca gacaagatta ctttattatc aaagctagtt tctattgtta ggttttttaa
    14701 ctcatcttca gatacatttc tgatgattat tttaccatct tgttcttcgt taacattaac
    14761 atttctagca atatcaacat atacttcaat tttatctagt ttttctgtca tctgtatgac
    14821 tttttgatct gcaagaatag ttttttcttc tccaaaatca ttagtatatg ttaaagttgc
    14881 ttgtggtgtc ccaaaactaa tgtttctgtt ggtttttaaa taatatttta atatttttgt
    14941 ttctcccgga taaattatac cagaatagga agtttctcca gatacagttt ctaaattaga
    15001 tattctcaag aagttttcat cagcataatt aattgttact tctacccctt ttgggctgtt
    15061 attttctgca taaatttcaa taggcaacaa agtgttgcta tgtgtctgag aagctagaac
    15121 ttttatatag aattcttctg gttttatttc tcctattata taactaccaa ccgtgctatt
    15181 gcctgacaca attacaatcg gcaatccatt atttgttata caaattttca aactattttt
    15241 ttcttgtatc ttacctgcta attttatgcg attttctttt agtatttttg cattttctat
    15301 tttattttca aatgttccat tgaaatacac agaaatacta atgttttctt taaccggaat
    15361 atagttttca attattaagt taagatacaa atcgctattt tctgtttgct ccattttatc
    15421 gaaattataa taattacata gctcttgatc tctttgcaca ctaaaattat cataatttaa
    15481 agaagcaaaa cttctttctt ctagcacatc tgccctaaca acgcccgcaa ttatagcaat
    15541 cattaaaata attagaatca atattttttt taacatagta aatcacatta aactgattat
    15601 atataatata tatacatttt atttaaatat cttaccttta aagaaatagc aaagaatcag
    15661 taatgtctta atatcttttg ttttttctcg tctttactaa gcttttttgg ttttttatta
    15721 aataaaaaaa ccagtaatat cgcgccacca agcccaaaag ataagatttg gcctattttg
    15781 ggattattgt tttttggttg tgttgttaag agaaccctag agtcaaaaat tacttttaga
    15841 tagtttctat tttcataatc tttttttata ttgtcaattg cttctttatt ttttgaaagc
    15901 actcgagaat ttaagacatc atttagcgca gaatttgaat ctttctttat ttgaataatt
    15961 cctgtattta ccaattctat ttctttattt aagtccatca acctttcaat agttgtaatg
    16021 tttataatat ttaaagcatc ttcgtttctt ttagattcgt actcgtatac tgcatttgtt
    16081 gcttcttctt tgtattttac ggtttctaaa aatgcaagtt tcttctttat tcttattgcg
    16141 tcatcaacag taactgccgg atagtattta acagaataaa ggtcttttag atctatatct
    16201 gaataatacc actctaattc ttgtaatttt tctaaaaaat cagatttttc aaagctttta
    16261 aaataagtaa accattctac atttgtttct gtttttttag cgagtgtttc atttatttta
    16321 ttttctaatt ctgtaattgc tgcagcgtta ttcggcgtgc tttccaattc taaaaattta
    16381 tcttgcagtt ctaagtccat aatatcataa gattttgcaa gattctttag gttatttagt
    16441 ttattttcat aattgtcttc ttcaagttca actagcacgt tttttgaatt tgccaggtct
    16501 tcgtataatt tttttgcaag ataatatgcg gtttttgggt ccgtaaaaaa cattttttct
    16561 attttcataa tttctacact gtacaccccc tctatattcg aatcactaat atctgcaagt
    16621 agcctttttg tattttctaa atattcaaat ttttgatttt ggctcttttc aagttctata
    16681 attttgggtt cctgttttaa tattgaaata atgtcatcat aactaaaaga ctcctttaaa
    16741 ttacattttt cataaattgc cttaaaggca gagacgcttt ctttagatat gtcttcaaat
    16801 tcgctattca taagcgcatt tatttttaaa agcaaatctt ttattttttc taaaagttct
    16861 gcatattcca ttatacaata cacgtctacc ctttttgttt caagcgtttc aaaacacgtt
    16921 ggggcgctaa tttgccctgc attcgcatag tagtcaatct caattttggt tccgtttatt
    16981 actaattttg ctaacccgct tttacattca gtaaaggaaa gatttccaca aactttttca
    17041 gaatgcgtat tctttaaatc atatctttct aacaacacaa gatttccatt ttcgacttgc
    17101 ccaatatttg tatcaagatc tgttttttga aaaatatgca aataatataa aatcttattc
    17161 tctttttgag tgctatattc tatggcgcct tcttcattta atacatatgt cgcagaatcg
    17221 acaactgccg cctgtccaag aaacagtgtg tcctttaagc cgctgactgt attttttata
    17281 attgtttcaa atagggtttt gtctatgtct aatttaatta tacgcgtagt tatgcttttc
    17341 ttattttcat agcctatttc aaaaagatta gtgccgctat atactttatc aaagcaagcc
    17401 gtttcctgat ttaattttaa gtttttagtt aggggaaaag caccttctaa ttcgagcagg
    17461 cttgcgtcta aacaaaactc agtaagtacg atatttgatg aatttacaag ttttaagaaa
    17521 taaacgcccc cctcataata tatttctttg ctcttaataa tttgctcact agttgcttct
    17581 aagaataagg cctttatttc tgcatttagt gtgtttattt cttttagata ttctttaatt
    17641 tctaaaaata tattgtaatt ttctttattc ttatattttt ctaatttatt ctttaatttt
    17701 gcaatttcaa tttgcatctc aaaattcaaa ttaaatcttt taagttcttc taatatatta
    17761 gaactatctg aataaatggc ccaaaaaagt tcaatctctt cagcacgtcc tgcactgtaa
    17821 ttaaattgat taattaaatc agtgcattct tcttgcgtcg taatattaat atctataata
    17881 gcgccagcaa gcgcaagttt ttctaaactt aaaaaacaat ctttttggac aagcgtattt
    17941 tttagttttt cgcaaatctc tatttttaaa acattatttg tttcattttc aaattttaaa
    18001 attaattctt taaaatatag attattatag tctttaatag aatcaatgac tatttcgcaa
    18061 tcctttatct cctctgtttc ggtttttgtc ttttcttcag ccaattctat ttgtttttct
    18121 atttctaaat taaggtcttt tagatatgaa agataatacc caaaccccat atctccagaa
    18181 gcatagtttt gtttatattt tttgtattct tcaagtttag atttgtctaa aataaaaagg
    18241 ttgttttcta ggcgttcttc tagaccatta atcctattta atgccaaatt taaatcttca
    18301 ttaattttat tatttaaaat tataaatctc ttaaacaaag agtcttctgt tccaatgtgc
    18361 ttgtcaaata atatataaag gttatagtta tctgttcttt taagatttgt gtttattctt
    18421 tgaaagtttt caaaagtaga taatttagag aaaacataat tactagattt agaaattact
    18481 ggcattttta gttcttgttc aggattatct atatattcag aataatacgc ataaatatcc
    18541 acataattaa cgctcgtcat ataacttttt aaaaacccaa aattgcttgc tagttctctt
    18601 gcagcaaatg cttctttttt taatacgccg acatagattt tatcatttat gctttcgtct
    18661 cttagctcat taatattctg attaatcaaa gcaaaagaaa cgtatatttc ttcttcagtt
    18721 atttcttcaa cgccctgcga tttaagatat atagcatagt cctttaatat ttctatactt
    18781 ttctgccaag ctaaatcttg atagttaaat attttagaaa tataaaacat taaatcatta
    18841 atacttctaa taatctttaa atcattttct ccagagcaat acgcatatat tttctgtatt
    18901 tctttttctg ctttattaaa atagtaaacg ctgcttgcta tattgttttt ataggttaaa
    18961 atcttatttt tagtttcaaa cgaaacctca tttaaaacaa aaaatccaga gttatttatt
    19021 tttttataac atgcggaaaa tccattacaa tcaggaatac tggttttaga gacgtctgta
    19081 gaagacaagc aaagctgttt ttgtcttgta gtaattacat ccaagcaacc gctaagtaac
    19141 acaagcagca atattgaaaa gagaacaatc catcgcatag atgaattaag aaattaagtg
    19201 caatataaat tcagtagtct gatatttttg taaaatctat aaaaacaata tgaaaaaaag
    19261 aaaaaacaca aaaggaatta gtattttact atcccttgag cattaattgt tattatttga
    19321 aacttaattt ttttctgtaa gagtagtgct agaacaacgc tgtgctcttt tccgcttcca
    19381 gaatgtatat ttaaatcaag ctctttagtg tctgttggta tttgtttttc taagtccgcc
    19441 atgatttcac taaacccttt tcttggatca attattatcc aatttattgg tttagtacaa
    19501 ttaaatttgt cttgtgccca ttcgttagat attaaataaa ctttatcata atctacatta
    19561 tcaattatgt gtgcgacttg tccccaagtg ccttttcctg tgcttaatat tcctaaaaaa
    19621 gccttcattt aatcaccttt ttttgtcata tatcaaattc ttttttcgtt tctttgtaca
    19681 aactcatgaa ttttttgcct ttttctgtaa tgtaataaac tttgttttca tttaccccaa
    19741 ttttcttgat taaatctttt tgaataaggg attctaatat attatataat ataatatctt
    19801 ttttaatata tatgtatttg aagacatcct tttttagatt aagaaaatcc gcacaatttt
    19861 gttctaaaat cgtcaaaatt gccatgtgtt ctttagaatt aacaatatct aataattttg
    19921 ggtcctctga aaacatttta attaagttat ctttattgtc gctcatacat aattataatc
    19981 ttaaatgttt ttaatcatat aaggttcaga tagagaatat ccttgttttt tatagtattc
    20041 tcttacgcca actccgctaa taatggcgat tttgttatat ccttcttttt ttgccaaatt
    20101 ttctgattct tttaatagtt ttttgccaat tcccctgtgt tgtatatttt tgtcttcaaa
    20161 tgctgttgtt tgtccataaa catgaagttc tcttatgatt gctgtttttt tagttatttg
    20221 tttaatatat ggcttattag ggtatcttaa tctaataaaa ccaagcagat atttgtcttt
    20281 ttcagcatat aaaaatattt cttttccatc agaagcaata tattcttctt tttttatttt
    20341 atatcctttt gtatcttcaa gttgcccctt aggttcttta catctaatgc aattacaagc
    20401 tttctgacgt ttttttaatt cttcttctac aagctgtcta aggttgcttt tctttatgcc
    20461 agcaattatt tttgttgctg gtatatccct ttgtattctc ataattctaa cccatctcgg
    20521 caccaattct ttgatatctg caattagttt gatcgttttt tcaagttcta gtgctttgta
    20581 ttctccagag taatacatct gttctagttt tgttcccgca attacaagac aagggtagat
    20641 tttgatcatg tctggtttaa aatcggggtt tgtaaatatt tccttaacgg atttaaggtc
    20701 ttttttataa tcacttccag gaagcccaag cattacgtga tatagtactt taaaaccagc
    20761 atcttttagt tctttagtcg ctttaattat ttcttttact ccgtggcctc tatttacaaa
    20821 ctttaaaaca ttatcatcta ttatctgtgc gccaagttct actctagttc ctccataatc
    20881 cagtaatctt ttgattgttt ttttatcaca aaagtccggc cttgtctcaa aggtaattcc
    20941 aattattctg tttttagagc ccattgcttt ttttttaagt ttatttaaat ctttttctgt
    21001 atctttagaa tttgtaattt cttgaaaaat agattttaca aaaccgtctt gatatttttt
    21061 agaagttgca ggaaaagtcc cacccattac aattaactct attttgttaa ttgggtgccc
    21121 cgtagatttt aattgcttta atctatcttt tatctgcgct cttggatcaa aattatttct
    21181 ttttgctctt aggcttgcag gttcaaaacc agtatagctt tttggcgcat tcttccaagt
    21241 tgggcaatat gtgcatttac ctgggcattc tcctggctta gtcatgattg caacaattgt
    21301 tacccctgac tgcgttcttc caggctttgt tattaaaaat tctggcggac tatctatccc
    21361 cagtgccaaa aacacatcag aagttagtgg aatcttttta agtccgtatt tttttgcaaa
    21421 tgaaactttt tttgcttcaa attgttgttt agtaaattta gatgacgcgc attttttaaa
    21481 ataactagat attctttttt tatataattc gtccatattt ttaaatcccc tttttaaaag
    21541 aaaagagata gttattctga tgcaattcta ataatattat taaagaagta tgcgggtatt
    21601 gcatttaatg caaaggttgg gaaattatat aggtttgcag catatagcgc agccaagtct
    21661 tttttaataa ataaatttat ggtttcttca tttctttcaa acaacgcctt tgtaaaaaag
    21721 gtagttgttt gtattatgct attttgggga actgcgtcag tatatacgaa taattgtatt
    21781 tctttgtctc caatagattt tggaataaac gaataattaa catcaataga atcgcttggc
    21841 tgtaaaacaa ccgttacttc tttttttatt ttttctgcat ctttagaatc aaaccaagtt
    21901 attggaagat tagaagatat tgtgattttt tttgtagtta ttgatttgtt tattacttta
    21961 attgttatgt cttttcttac tccaaatttt gtttctttat catacggtaa aacaaaagaa
    22021 gatattacgt ttgtcgcaac agtcattttt atatatgcac tttcagtttt tcctgtttct
    22081 gtatttttta atataatttt aacttgttgt tctcctgaaa cattttcttt aatcttaaca
    22141 atagtgtata tgctttcttt tgttttttgt gtgttttcaa agaagagata atttgagcca
    22201 taattgtctg tagtaatcaa atcgtatttc tcatcaactc ctaaaaagaa actaaccaga
    22261 aaatattctc ctggcgcaac agttccaagc tctattgctt tatttggatt aatgtctgtc
    22321 attattggtt caagcatttg tatagcatga atgctgggta aaataaataa taaagagatt
    22381 ataaaaaata tttttttgtt aaacataatt tttcacgatt tttttgtgca acatatatat
    22441 tattattgct atatataata tatactaaat tatttataaa tctaattaaa atcaacgttg
    22501 tgtgaaaaaa catgaataaa acattagtgg attcaagatt tgcgatgcta aaaaggcatc
    22561 acactcaaac acttaaagat gcaatatcta aagaccaagt tgatgcgcta gtcattcctt
    22621 ttcttttaaa ggtcacagaa ataccagaca tttacacctc tagtagctgt tctggaagat
    22681 tgttgcttct tggtggagac aaagatgaga acaaaaaggt ttcaggtttt gttaatcgct
    22741 atcaccggca agttactttt gaagagataa aaagagacgt agataatttt aataccggtt
    22801 atttgtggct taaagttgaa ccctttattt ttcattttgc aacaaaggac tatgataagg
    22861 ctaaagatat tttagatttt tgtagagcgc aaggacttaa aaaagcagga ataattagtg
    22921 ctaaagaagg gcgctttact tgtgaagtaa cacacacagt gtttatgtct acacttatta
    22981 aaatagataa aaaacagctt atttcggatg aatatctaaa gcttatagta gatattgcaa
    23041 acaaaaaatt ggctgcaaat tttaaaaaat tagaagattt tgaaaacgct tttcttaagg
    23101 cgttcccctc aaaactaaaa ataaaaaaga aatagttatt ttcttttctt cttttctaaa
    23161 taactctgta ttaatggcaa atattcagca agttcttttt tttcttttgc gtttattcta
    23221 ggatttccag agttaatttt ttctgagatg ttttgtaaga gacttgtaag ctccattgga
    23281 aagattatct ttgaggattg cccttctcca accttttcaa gcgccttaat ataatagtat
    23341 aaaatagttt tatctgaaag gctttctgtt gcttctctta tagcattaat ttttgccttc
    23401 tcggattctg cttcttcaaa tattgcaagt ttcttctgaa ctgccgcctt ttgcttgtgc
    23461 attgattcta caatttcttc tggtatttgt aagtctctaa tttctacaga gtttacccgt
    23521 attccccatt cctttgcaac gccttttatt tcttctgtta tttttttatt tatgttttct
    23581 gtatttgata taacatcagt taaaacaaac tcacccagta tgtctcttag catcgcaatt
    23641 acaaaataag atgctgcttt tttatagtct tcgactttta caatagaatt gataatgctt
    23701 tctttattgt tttctataga aaggaaaaga atggcgtcaa ttgtaacttt aatattatct
    23761 tttgtaatta tctgctgaat aggcacatcc acaacttgcg ttcttaaggt aacataagaa
    23821 tatttttcaa taaatggcaa aactaaagtc cagcctggcc ctccaactct attaactttt
    23881 ccaaatctaa atattactgc tctttcatag tcttttaatt gtaatataat attggcacta
    23941 aataataaaa atataaaagc aataaagatt agcacaataa gaataatttg ttttatgctt
    24001 tcgtctatta ttaaaaagat agagccaagc aataaaaatc caaaaataac cgttccaaaa
    24061 aatatgtttt ttagtttttg ttcttccatc tttattccta cttgtatttt tgaatttaga
    24121 tattctttta aatgctttta tatattaaat cctatatata tattatagat agaacattta
    24181 ttaatctttt accttgttaa aattaataag aggcaatttc cttggtcaaa gaaacaacaa
    24241 aaaaagctaa acccgaagaa gtagtaatag ttgagcagaa agtgcagcca gcacaagagc
    24301 acatacttat acttacagtc gatagagata acgacattgg caggaaaatt ggtgtttctg
    24361 ggccaattgt tggttatgaa agtaatttaa agatggcaac tgacttgtta cttaaagatc
    24421 cagaagaatc agatgcaaat gcagtttttg caattttaaa aaaatattct gaattaaaaa
    24481 aagattatgt tgttgaagtt gcaacaatta ctggacactc aaaagagaat ttgttctttg
    24541 ctgataaaaa tatcgcttta caactaaagc aagttctaga agatttccca gcaagcgctt
    24601 ttgttctaat aactgacggt gcagaagacg atcaagtagt accaatagtt caaaactttg
    24661 ggccaataat ttctaaagaa gttgttattg ttagacaatc tcaagcaata gaaagtatgt
    24721 attatacaat taagaaagca ataaaagatc ccgcattttc aagaatcata tttggaattc
    24781 ctgcaattgt tttgttattg tttttctttt tcaagtctta tgcttttcaa attatcgccc
    24841 tggtactagg cgtatttttc ttaattaagg gtttccattt agaatcaaag atttctaatt
    24901 tctttaattc aatattttct aagttttcaa ttacaaaaat tagctttccg ttttatgtgg
    24961 ccagcgtttt tttcttgatt tatgcaggaa taactggcgt taatcttttt ttagcaaacg
    25021 attcctttat actttggcaa agaataattt atgttttaag agcgatactt ttgtatttgg
    25081 tattttgtat tgggtcttat gttgtcggta atattgtaga tttgttttat acgcgcgcgc
    25141 tatataagct aggtcacgca atttttgcaa ttatttctac atttgtgttt ttgggattat
    25201 tagatttgtc tttgcagttt gttcttggag aaacaagcat gcgtatgtta attactgtta
    25261 ttttgatttc atcggtattc ttatttatat tatacaaaat tactagcgtg tttgatataa
    25321 caaaagacat cactgaatta ttggttggcc ttccagtgat atccaaatac ggggtgtggc
    25381 ttggagaagt tatagcagta gataagacaa agcaaacaat aagttttaaa agcagaaacg
    25441 gcaaagtaat aactgtgctc tcaaagaagc actttttcat acaaaacggg caagtgataa
    25501 tctaaaccgg tttgttttcc aaactttttt aaaggttttt gcgcgccaat ataaatagaa
    25561 acatatatat attaatcgtc taacaatata aattgaaata tataatattt taaaaaaata
    25621 ataataaaag agaggttata aaacatgtac aaatacgcaa tagcaagaca gcttgcaaca
    25681 aagaaattaa taacaaacag aggagacaat attggaagac ttgtagattt aatggttaac
    25741 gaagtatctg gagagataga ggctttatta gttgaaatag atccttcatc aaaagtagtt
    25801 aagcaattag atgttcaagg aaatctttta gaaattccat acagaagtgt aattgccgtt
    25861 tctgatgttt tcgtaattga tgaaaagacg ctattaacaa aagacgaagg aacttttaat
    25921 taaagttctt tattttttct atttttttaa ttgttaaaaa agaaccttaa cttctgtggt
    25981 tttaatatgt ttgcggattt aaagaacaag aaagcaattt ctccaattat tacaaccatt
    26041 cttttagttg tagttggtgt tattcttatt actgttgttc ttaattttgg aaaaaacttt
    26101 acaactaaat cattagacca gacaactaac tttggcagcc ttaaggtttc agatgcaaca
    26161 aatttcgttt atccaaaata tgcaaaagac ggggtattac aaataaatta cgctccgcca
    26221 gtaaaattag aaaatgaaag tattacaatt acacaatatc aattattaga tgttctagat
    26281 tctgaagtcg tgactttaca aacaccagta actttaaaca acaacagtct tagtacaatt
    26341 caacttgatt gtctttacga atatgcatta agtaagccag attttactat tcagttaatt
    26401 actgctcaaa atacttatat tgaagtaaaa ttaagagatc caaatatggt ttgttctcct
    26461 gggggaacag gcacaattga agacccaata attgtttgta atgctgaaga ccttaatgcc
    26521 atgcgtcttg acttgatgca aattacgctt tagggaaaga tattgacttg cgctgctttt
    26581 caaggcagag agcagatggg tggcttccaa taggcgacta tgaagattta ttttctggct
    26641 catttaatgg caataaccac aaaattagta atttatatat ttctgattct gatggagttg
    26701 atttgggttt gtttggatat tttgatgccg gagaaataag taatctcggt ttagaagatt
    26761 taaatatttc tggcaaactt tatttaggag gtcttgttgg agaaatggat tctggagaaa
    26821 ttacaaattg ttatactaca gggaatattt atgaattatc tgggggagga cgtattggtg
    26881 gaatagttgg gggtaattgg ggaggataca tcactaatag ttattctgaa accaacatat
    26941 atgccggatc aggcgatttt tattatggtg gtttaattgg gaataataac ggagatgtag
    27001 aaaattgtta tgccaccggc tttgtctctg gcagatcata tgtaggaggc cttattggag
    27061 ctaattttgg gggaagcata actaatagct acgctacagg agacgtaaat gttttaaaca
    27121 catatgcgga tgctggaggg ggttttattg gagataatga aggcacgata agcaacagct
    27181 attcttttgg ggacgtcact cgtgttactg gttcaaatac cacatttgga ggttttacag
    27241 ggtataatgg gggaacaatt acaaaaagct attcaacagg cagagtcata tatgcaggaa
    27301 caacaaaccc aacaaataga ggcttttcat tcgactccgt ttcaaataat aatttctggg
    27361 atacacagac ttctttacaa gccacaagtg gtggcggcgc tcttggtaaa acaaccgcag
    27421 agatgaaaac tccaagcaca tatactgatt gggattttat aactgtttgg gcaattcatc
    27481 caagcaagaa taacggttat ccgtatcttc gctggcaaaa ctaaattgat tttattttat
    27541 tcttttatct ctaaaacata gcttctttcc ttagtgtcta tgcttttgat ggaaagttct
    27601 ttttgattct tagagaagta gtcatttaat ttctttataa tttcttctgg cgcgattttc
    27661 ttttctcgcg caagtctaat ctgttcgttt actttctcaa ttatctgaaa gtattcatag
    27721 atcttctcgc cttcaatttg aagattagaa gcaatctttt catattcttt tcttgtcttt
    27781 atctggctgt caagaattga tgtttgtttg tttttgcttt tcttttctat ctttgtttct
    27841 tcggttttaa gtttttctgc aaagtattca gagatgattt taaaaaaacc aaacgaattt
    27901 tgtttttctt ttccttctgc aactaaaacc agagactcgt cttcgttctt tcctttttca
    27961 agagcaagag atgggcgcct taaatcataa agctcttttg ttttttcaac aatctctttt
    28021 tgtgtttttt cttggtttat ataacctgaa taaatattat attcttttac aaaatagcta
    28081 atggttgctt ttaagttttt atcttttatg tcttcaagta gttctttttg tgttttttct
    28141 gtaatttctt ttccttgagg aaaagagtat ttctcgtttt tcttaataat tctgtctttc
    28201 cattcttctt tttgttttga agttataatc tttaattcta aatccgtaag tataatatta
    28261 gaattactaa agaactcaaa gataagataa tagtcatcaa gcttaaaata aacaactctg
    28321 ttatttttgt cttgcttaat ttcttgtatc ttttgattat agagtttctt ctttagaaat
    28381 ttgataagcc ctccgctttc tggattaacc gggtaagcaa gttctgttaa aaataaaatg
    28441 ttgggagata caatcagttc ttttgtttta gttttatgta tttttatctt aattaaatta
    28501 ttgtcttcat gattaacaag ttgaagatta ttaatgtagc caccaactac ctctgcttgc
    28561 aattctttta ctatataaaa aaaagcagtg cttgttactt ctagtttttt catatctttc
    28621 ttattctgca tctatgtagt tattttgatt atagcgatat atgatttttt ttatccttaa
    28681 tccattaaat ttataatcct ctaataattt cttttcatca gtatttgtaa acatctcaaa
    28741 tggccctttc tgtgttaaat caaaaatata aaattgattt ttatatctcg tcttaacaaa
    28801 cgcgtgcgtc ttgtcatctt ctaaaagtat gacgtaaacc atcgcttctt gatcatttag
    28861 tccttgcata attgaacaaa gaaatgcaga aatgtcttgt tcatcagcaa gcttgttttt
    28921 caaaatgcaa gttgcatcaa gccaataaag tatctttatg tcggttttaa caacttcaat
    28981 ttctgttttt aaaaacgaat agatttttct aagtgtttct atataatctt tattatattc
    29041 atagccaata ggcttgtgtt ttaatattac actttttatt gttaaatcat taggattaac
    29101 tgttgctttt atttgagaaa tggtttttcg ttcttgctca tttatcaatt ctttatattt
    29161 atctaaaatt tgttcataat atttattttg aagattgtcc ttaatagacg ttgaaagatt
    29221 ttttgtccct gaaaggtttt taataaagtc ctctagttct tttattctgt tttttagatt
    29281 gtctctttct agtttagttt cgttaagctc tcctttaagt tttatgtttt gaaatcttag
    29341 gtcgcttcca gtgtcttgcg ttttctgtaa ttctgtcaat ttagaaactt cgccatttat
    29401 ttcgttatca atttttgcga gttctgttgt tatatttttt aaattattag aatacacatc
    29461 gtcgctaatg ctttttgcgc tatatttata agacaactct tctttccttt ttagtaactc
    29521 ttttttttga gtatttaatg cttctattct ttgccaagaa ttttgttctt tagttacagg
    29581 aaccgtaatt ttttttctaa aattaataaa agtgaaagcc acaccaaaaa ctatagcaat
    29641 gcaggtgatc attatcattt gaattaattc aacatccatt tttatgctct tcttttattg
    29701 ctaatatata ttatattaga ggcgatttac tttatatata ttatgatttt tacgatattc
    29761 gaataaaaat ataaaataaa gtttggatta tcacagatta tccatttata aagttttacc
    29821 ttctatatta tatataatta gataaaaatc cattgcatac aaagaccttt aaggtgcaat
    29881 agattttgat tataattttt taagaaatct gtaaaaaaag ataaggagga ataatacatg
    29941 gcatcaccaa aaccaaaaat taatgttgtt tttattggtc acattgatca cggaaaatca
    30001 acaacagtcg gtcgtttgat gtacgacaca aaaaacttaa gtgaacagga ttatagaaag
    30061 ttacaggcaa tggcagaaga gaaaggaaaa ggaacatttg cttttgcata catgatggat
    30121 ataacaaaag aagaaagaga aagaggtatt acaattgatg taaactataa gaaattagaa
    30181 tcagataaat atagttttac agtaattgat gctcctggcc accaagacta tgttaaaaat
    30241 atgattacag gaactgctca atcagatgct gctgttttag ttgttgcttc aaaagacgga
    30301 gtaatgtctc aaactaaaga acacgcattt cttgcgcaag ttatgggtat taaacaagta
    30361 attgttgcag taaacaagat ggacgatgtt aactacggcg aagagggctt taataaagtt
    30421 aaagcagata tacaggcact tttcaaaggc aagtttaaag atgatcaata cgtagttata
    30481 ccaatttctg cgtggaacgg cgaaaacatt acaaaggctt cagacaagat gccttggttt
    30541 aaaggaccaa gcctatttga agcaatgaat aatttcaagg ctccagaagt attaacagga
    30601 aagccattaa gactaccaat acaagatgtt tattcaatta aaggtgttgg tatggttcca
    30661 gttggtagag tcgagacagg aatacttaga ccaaatgaca aagtcataat aatgccagaa
    30721 ggaatcgcaa cacaaataaa gagtgttgaa gcacaccacg aacaattacc tgaagcacta
    30781 cctggagaca atattggttt caatataaaa aatgttgata aaggcgcaat taagagaggt
    30841 tctgttgttg gaccagagaa tagcccacca acagtagcag aagaatttac tgctcaaata
    30901 attgtgttaa atcaccctac cgcaataact gcagggtata ctcctgtatt tcacatacac
    30961 accgcacaaa tgtcttgtgc aattgttgag ttaataaaga aagtagctcc aacagttgaa
    31021 gacgctccaa agttcttaaa gacgggagac gcggcagttg taagaatcaa gccattacaa
    31081 ccacttgcat gtgaaacata taaagacttc ccacaacttg gtagatttgc tatcagagac
    31141 atgggtaaga ccgtagcagc aggtgttata caggagataa agaagagagc ataattttgc
    31201 tttttttctt taattttttt ttaaaagtga taaaaacatg caaacagcaa gagtaattgt
    31261 tacaggaact gattacaaga aagttacaga attttgcaag gaaattatag atgtggcttc
    31321 aaagagtgga gttaagcaca caggaattat ccctttgaag acaaaaaaat tggttgtgcc
    31381 agtaagaaga ggagtttctg ctggtggaac tgagacttat gagaaatatc agttacgaat
    31441 ccataagaga ataattgata ttaaagcaga cgacaagatt cttagaagaa taatgagagt
    31501 cgatgttcct gcggatctac aaattggaat agaacttaaa aactaaatcc attttgttag
    31561 catcataatt tagtaaactt gcaaatctat ttatctttag tttttaaaaa aattaaaaat
    31621 ttgcaataaa atgtttaaac aaacaaaaaa aaatagtttt gttgatgaga gcaacaaggt
    31681 aaaaattatt tatagtaaat ctctattttc taaactggac aaatcttgta tagaacaact
    31741 ttattctatt tccaaattac caggaatctg cgattatgtt tatggctttc cggatcttca
    31801 tgtaggccac ggcgcaccag ttggttcttg ttttgtagta aatgctgata atgatccaat
    31861 aattagtcca aaagcaatag gcacagatat aaattgtggt attcgcctta ttagaactaa
    31921 tcttaaatca aaagaccttt ctgaaaagca atacaaagaa atagtaaagg cctttgaaaa
    31981 acttccgctt ggtctttcaa aagacggcgt aaagataaca aagcaagact taactgacat
    32041 ttgttatcaa ggcttagaat gggcagtaaa aaatagttat gctgataagt tcgataagtt
    32101 atatgtttat agacagggtt gctttcgcga cgcaagtcca aaatatctat caaaagcagc
    32161 aatagaaaaa ggcctaactc agcttggaac tcttggtcaa ggcaatcatt ttattgatct
    32221 tttggttgta gataaagttt ttgataaaaa gttttgtaaa aggcacaaga ttgaaaaaga
    32281 ccaaattcta attgtgcttc acactggttc tcgtggcctc gggtatcagg ttgctgaaga
    32341 gtttacaaag atatgtaaac acaaagaccc aattgagtat ttatatctaa attctaaaga
    32401 tggccagcac tattataaga caatgtgcgc cgcagctaat tatgcttttg taaatagggc
    32461 ggttctttct aaaaaagttg tagacgcttt agaaaaaaca ctaaatcttg gacgcggaga
    32521 catcaacgct cgccttctat acgactgctc acacaatttt gcaaaacttg aaacccacaa
    32581 taagaaaaga tatttggtta caagaaaagg ggcagttaga ggaataattt cagaaaggct
    32641 ttctgataat tcagagtttt tagaaactgg aacgccagta attttgccag ggtcaatgct
    32701 tgacgatacc tttgttattt tgccaaaaga aaaaatcaga acagaaacct tttccacgct
    32761 ccctcacggt tgtggcagag ttatgtctag agaaaaagca aaagagaaga tctcagcaag
    32821 cgatttaaag caaaagctat taaaaaaagg aatttatctt tctggtcatt ctgaaaatgt
    32881 cctaagagaa gagcaaccag aagcttacaa gtcctcaaaa gaggttgttg attcaatggt
    32941 ggacgctcgc ctttgtaaaa aagcatttag tcttagacca aaggttgtaa ttactggtta
    33001 aaaaaatctg tgaaaattta tgcacgacat aaagctactt tcttgtagat ctggtgagca
    33061 ttttgcaaac aacgttttaa aatatcttaa tgaattgctt ttagaaaaca acgagccaga
    33121 aataactttg actcctattc aagagattag atttgcaaat acagaaataa aatcaaatat
    33181 tctagagtct ataagaggaa gtaatgtctt tattattcag gatgtttcaa attcaattta
    33241 tgcaatgtcg gttgatgata atctcagagc tttaacaact ttaatcgatg cagcaagagt
    33301 tgcaagtgca aaagaaataa cagtaattgt tcctgtcttt ccttatgctc gccaagacaa
    33361 aatttggggc agagaatgct taacagcaag gcttgtttct agagagatgg aactagccgg
    33421 tgcaagaaca atcctttctt tagatattca caatcctgca attgctggtt ttttcaagta
    33481 tgcaattctt gaaaaccttt atgctcgtaa ggctattaaa aaatacatac ttgataatgt
    33541 ttctcttaat aatcttttaa tttcttctcc agatgttggc ggtatgaaaa gagcaacagt
    33601 tctagcttca aagcttggtg tgggtgcaat aacaatctac aaagaacgcg actattctaa
    33661 aatcaaccaa gtagaaacca tggaagttat tggtaaagtt aaagataaag acgttttttt
    33721 gattgacgac atcctagata ctggcgggac agtctataat gcagtaaaat ctcttaaaga
    33781 aaaaggcgca aataaagttt attttgcttg ttctctttgt ttattaaatg gaagcgctgt
    33841 tgaaagaatg gatgaacttt atgataacgg ccttcttgaa aaaatcatag caacagacgc
    33901 agtatatatt gaaaaagact ttatagagaa acacccttgg ctagaagtta ttccaataag
    33961 tgattacttt gcaaaagcaa tttttaatat tcattttaag aaatccataa atcagttgtt
    34021 tgacaaggac taatttttag gttataatct gattataatc taattcttat ttaaatattt
    34081 ctaggtttgg gcgaagaaga ggaaaagata tggaaaacga aataaagtgt catttttgtg
    34141 gcggaacagc aataattaaa tatagaaatg tggtccttga tgatgggaaa ataacaataa
    34201 aatacgagtc gtattatgaa tttaagaaat gtaaagaaca gttttgtaca agcgagcaaa
    34261 tgtttacttt agataaaaga cttcacgagt aatttgcttt taaaagaaac gttattgtag
    34321 ccggtaaaag tcttgctata actcttccgg cagacatagt aactcattac aaaatcaaga
    34381 aaggcaaaaa agtaagtatt cttgcagaac caaagggtct aaagatacgt tttgattaaa
    34441 aacaacaact atttataaat tatttgttgt ttcttttatt aaaacaaaaa agttataatt
    34501 tatggaatct acagcaatct taattgtttt aattattaat tctgttcttt tgatattatt
    34561 atttgtattt atgataaggt ttgttgttaa taaatttaaa gaacagataa cagacacaaa
    34621 tgaaagatta aaagacatag aggcaaaata caataaacta gacattcagt tctccagaat
    34681 ttctgctctt catgtttctc aacagaaaaa tgatatctca aaagatatag aaaaagtaaa
    34741 gcttgttgtt caaggcatag ataagaaaac agaaaagatc tataaaagaa cagaaaagat
    34801 gtacggcaag aagcaataac tttctatttt ttaaatgctt tgttttattt attatgttat
    34861 atgcttaaat gtgatgtgtt atgggtattt taagaagaac aacaactata aaaaaaccag
    34921 cagttaaaag aactgtcaaa ccaaaagcaa ctagaataaa agggacaata aagatatctg
    34981 agccaacaag agtactttta caaaagaaaa atcacgtctt tatattagaa tcaaaattta
    35041 aacccctagc aaaagaatat cctgaatcta agaagtatgt tgctttaatt tcaaatgttt
    35101 atctaaatgc agaaaaagaa attttagctc taagagaaag agccaaagga caccacaata
    35161 tttctccatt aatagatctt ttagaaggcc gctacaagca agtatctaaa aacctaatta
    35221 attttattaa tgagccaaag ccaaatattt ttagattcat ggaagttgag atgcaggctt
    35281 ctaattattt aaaagagcat aagcaaatag aagaaatatt aagccctaag ccaactatta
    35341 gaggaagcgg gcatcctgga aaaggcgatt ataatccaag aagacgcagt taaaataaag
    35401 ttttttgaac aaatggtatt tcttgttctt ttaatatttc tatttcttta gctttttttt
    35461 cttcttcaga aaaacaaatg aacggcacgc catagtgtcc accaccgcct ggtctaaaaa
    35521 ccactagccc ttttctaaaa gcaataacat atttagcaac gtcttcatct atttctttta
    35581 gcttttcttc aggcagttct agcatgattt ctatttctgt atattttgta attagttttt
    35641 tgtatttctc taaaactctt ttattataaa gttctccttt cccaattttc atttgaataa
    35701 tttgagcaag tggtattaga tatttatatt ctgg
//
LOCUS       DAOOON010000008        35519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_1398_length_35519_cov_14.247942, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000008 DAOOON010000000
VERSION     DAOOON010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 35519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35519
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1398_length_35519_cov_14.247942"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLK55_02030"
     CDS             <1..786
                     /locus_tag="PLK55_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ35739.1"
                     /translation="KKADIGIAMGIKGTEATKEVADVVLKDDNYSSIISAIKEGRRIY
                     DNILLFVKYMLAVNFDLLIIVALLTILAFPIPLLALQILWINLVTDSLPAIALGRKPA
                     TKDIMLRKPNKHTGNSFKSFTTFIIIALVVKIIGEIILFSYGLNIDTSMGINPFDISQ
                     PSYARTLLLTGIVVFELIFAFACNSEGSFSPKRLFSNKMLIGSVILVLALQLFLIYNP
                     FMQNAFNAVALSFKDWLMVLAFGLFSFIIIPITNFLEKIRSKS"
     gene            complement(783..1526)
                     /locus_tag="PLK55_02035"
     CDS             complement(783..1526)
                     /locus_tag="PLK55_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOZ35740.1"
                     /translation="MKNTKILITIPCYNEDKKLEKNITRLNSFLSKKLKEPYLIDIAD
                     NNSKDNTKDIAKKLEKKYSTVKYTFVKEQGKGIAIKYSWHNTGKNYDILSFMDADLAT
                     GIDAFPGLIKKITKENYDLVYGSRYHKESKTKRTIGRYIISKGYLFLQKMLLGTSFTD
                     TQCGFKAINKRAYYEIKELFLKKRLSGDRNNMFFDTELLVLAKYKGLKITDYPVTWKE
                     QKETSVKIFKYSILFLRYLFYFRKEIKRN"
     gene            complement(1545..3023)
                     /locus_tag="PLK55_02040"
     CDS             complement(1545..3023)
                     /locus_tag="PLK55_02040"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOZ35741.1"
                     /translation="MNSKTIMGLLKKYKFVLFIFLFTTLFFIIQHQFTISWDFISYVL
                     NAKNMFNSGLYFETFRPPLTSFIIGLFAQILGYAAAEIIYIIIVSALFCLSIILISKQ
                     LNFNTELFYLILFTPFVLYFGQINGTELLSVALLLLGIYFIIKNNWISGLFIGFSALT
                     RYTGIVFGVILLFHKGIDKKIKSIILFCLTFIPWFIYSKLKFGNFFTSIADQYHQNIT
                     SRLDIAQAPKLIHFISVQSILFVFTLIGLLYILYKLIKKNKNAKPLNNSFSIIMLLLT
                     IYTIHTYFSTPFKDMRYLFTLMLPIGYFTYVGITSIAEKIKKHKIKLHRIYLITIYIL
                     LIFSVLISSIKILEETYKLDYPYITRYKNAINTIYKNNLEDCVMYSDAWTFYNYLDSN
                     IIMLPIEIYIKDIPNYQDKVLFLYTPTRSKEQIIALENQLPTIIKTNNIMILGDLKNC
                     KLKENYVELFYIHNSLYTNPCNILFNKIKPIENICYFINSEK"
     gene            complement(3043..3786)
                     /locus_tag="PLK55_02045"
     CDS             complement(3043..3786)
                     /locus_tag="PLK55_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOZ35742.1"
                     /translation="MMSIYIYTLISIFVVSIISIIGITLFFTKDKHLPIVLLFLISFS
                     AGALLGDSFLHLLPEAMESGINFIPLAIISGILAFFILEKIIHWRHCHQPTSKEHPHP
                     LAAMNLLGDAFHNFLDGIIIATSYVVSIPLGIATTIAVLLHEIPQEVGDFGVLIYAGY
                     SKKKALLMNFLISLTAIFGGIVGLLLSKSITSFAGIMIAFTAGGFIYIATADLIPEMK
                     KDIGLKKTSIQLIGLLLGIGIMLLMILLE"
     gene            3856..4647
                     /locus_tag="PLK55_02050"
     CDS             3856..4647
                     /locus_tag="PLK55_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35743.1"
                     /translation="MPRKFGKIREPIISIKRIKRGVPEKEEWPMSFGKKERFISNPLY
                     LDTVVNINTKNKSPKILIMGPGLGEEIIEFDSFLKTHKHINPQIESLGITNQLTKDAL
                     KTVKKDHSLGIALEEIDPENPIHKQAIKKLIGRFDYIIAPVSVGIHTRYPAYNCFLCA
                     LALKPQGEARINIMNPESIKSHMDLYKHQLTETSEQYTPTEIRNITANFKLAKLNYNQ
                     VSKIEKIIYRMLNSYFKKDVKDQFFIKIRTDSSSKHQIIVIKRNY"
     gene            4659..5123
                     /locus_tag="PLK55_02055"
     CDS             4659..5123
                     /locus_tag="PLK55_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOZ35744.1"
                     /translation="MPETIKVNYKQVENFCLEIFKDISKTKFQPDCVIGVSVGGVFPA
                     IHFARLFNTKNLLTIAVKSYDGKKRKEIQVINLPKKESLLGKKVLLIDDISDSGSTLK
                     FIVNLLKKEYKVKEIKTTTIFVNEEHCKYYPDFYSKKVNKWLDFPWDRFEKK"
     gene            complement(5156..5227)
                     /locus_tag="PLK55_02060"
     tRNA            complement(5156..5227)
                     /locus_tag="PLK55_02060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5193..5195),aa:Thr,seq:tgt)
     gene            complement(5287..6501)
                     /locus_tag="PLK55_02065"
     CDS             complement(5287..6501)
                     /locus_tag="PLK55_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ35745.1"
                     /translation="MSSEKYKSLINLTIKNIFRKKLRSTLTILSVIIGVASVVALIVL
                     SEGMLAAVTDQFNKMGANSIFVMPAFNGNPNTSGRVSEENRLKMSEAYSISKIAEVEE
                     VYAMNFFTAKGEYKGEELFVYVVTIPSDKLDSMFKFFDVRIDKGKSFDGKDGYIAIIG
                     PYLAKEAFNREIKVGDTIKINDVDFKVGGILEAVGNESDDSSIYIGRKAGLAVDDYGE
                     TVNYMMIKSKEDADADLVVKKIERILKKTREEDTYTVISSESFLELIRRVMNMMRSIL
                     IAIAGISLIVASLGIANSVFTSVLERTKEIGVLKSIGARISDITFIFVFESVVLTVVG
                     GIIGFFAGIGIARLVILYAATHNFTMLTIHITPWLVIMTLLLSLIVGLVSGLFPARAA
                     AKMNIVDALRQQ"
     gene            complement(6502..7767)
                     /locus_tag="PLK55_02070"
     CDS             complement(6502..7767)
                     /locus_tag="PLK55_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35746.1"
                     /translation="MKTKYIMTLLFSIILLNGIYAATTDYDSINLVADTYNYSPVPVS
                     PGEEFELWIQLTNNSNVLAENVTYILDLEYPFSVADFNNTEKTIAMLAPYQTTIIKYN
                     LTTDIKAQDNIYEIEFKYKPGQSQVYNIENYSIDVSSGSTVLEITDTKLSEMSIGSDG
                     ELELTIKNLGAKTVRDVFVTLEDSTGDYISVLNLKTQYFENIGAGQEIKAKYKLNVAK
                     TITQNSYPLPLTLRFTGQSGEETITRDIGVKIDDNPKLVLNIVSIGTNNNNKIYIGNK
                     EKIDLEIYNVGNIDAESVYAEMTSTITTETPKYFVGSIEKDNYDSVALEFNTNADIKP
                     GSYPVEITIHYKDSALKEQEIKKGITVEILKNPKTKSVMSGIISIVIGILIGIIILAL
                     IILFLRWANKKILIPAFGGLYQNVFKKRK"
     gene            complement(7827..9023)
                     /locus_tag="PLK55_02075"
     CDS             complement(7827..9023)
                     /locus_tag="PLK55_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ35747.1"
                     /translation="MIGIFNFVIRNIRRRKLRNWLTILGIVVGVIAIVSLISLSEGLK
                     NTINEEFNSLGANKITITSKYAGFAGSSSGKGLTDEDIKTINKISDIEFSSGSLNGYI
                     ETKYAQETIMISYTSYAAEYLQEMLEQNNLEIEKGEYITDEKSKAVIIGYNYTDTEKA
                     KDLFGKTLDIGKKIKIATSDYTVIGILKKTGDSRKDNVMYLSNENLQEITESETYDTI
                     FAIVKPGKDIENAANRIEIKLEKARDAKDVTVTSPIQEAKNREETLGIVTIVVVGIAC
                     ISLLVGGVGILNSMYTSVFERKKEIGVLKAIGAKRRDILMIFLLESGIIGLLGGTIGV
                     ILGFSLAGLVKLVAYQVGITISISITASIIILALGFSFMIGVISGMIPAYLASNQEPV
                     DALREE"
     gene            complement(9025..9732)
                     /locus_tag="PLK55_02080"
     CDS             complement(9025..9732)
                     /locus_tag="PLK55_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOZ35748.1"
                     /translation="MAIKEKNVVELKGVYKIYCMGSEACVNALNGVDLKIKPGEFLAI
                     MGPSGSGKSTMMHIIGALDLVSKGQVFLDGTDVSNLSEDNLAKIRGQKIGFVFQDFNL
                     IPTLTALENVMLPMLFQENNKPLEIQEKHCKALLTLVGLGERITHRPTELSGGQQQRV
                     AIARALANDPEIILADEPTGALDSKASEEIMDLLKDLNKKGKTIILITHDRTVAHLAR
                     RQIHLKDGKIIEDKITH"
     assembly_gap    9937..10036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10038..10120
                     /locus_tag="PLK55_02085"
     tRNA            10038..10120
                     /locus_tag="PLK55_02085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10072..10074,aa:Ser,seq:gct)
     gene            10361..11017
                     /locus_tag="PLK55_02090"
     CDS             10361..11017
                     /locus_tag="PLK55_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35749.1"
                     /translation="MKHNQRKDKFNRFYKSNWFIIWVVIYGILYYLSTLLITALGISN
                     YYIKVILIGILISIIARAISTHIHNSNFKLNFNLFIWTLVYILLLFGFRFILSYFALN
                     QWLAIIVIALGFSIFTHLIKKIPFEILAIICIIISWMFAALLYVNENGFDLSKYIYTS
                     NSLSENSDLNRNSLRLASGSGSKDYTPHTNYIQTHQPAKLESKDYMGSNLIISYRPFI
                     "
     gene            complement(11054..11404)
                     /locus_tag="PLK55_02095"
     CDS             complement(11054..11404)
                     /locus_tag="PLK55_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35750.1"
                     /translation="MIPQYITNLLIALVFTIIIECIIAFFFGYKDKKSLVVVLLINLL
                     TNPLLNYILLICYNFQIVYNVIFAITIVLEITIVFVEWKILFYVLEKDKKKLLLLSIV
                     LNLASFLSGFIIPL"
     gene            complement(11620..11826)
                     /locus_tag="PLK55_02100"
     CDS             complement(11620..11826)
                     /locus_tag="PLK55_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35751.1"
                     /translation="MMNNKMLLVLIGLVSLLNIIYADVVLAPTPFEYIIPVAAIIIVG
                     AVVVGIICVAIFLIKKIKAQLTKK"
     gene            complement(11823..12887)
                     /locus_tag="PLK55_02105"
     CDS             complement(11823..12887)
                     /locus_tag="PLK55_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35752.1"
                     /translation="MFLINFMQVVKKNIFGNLMVYSLAHFLIDATCAAVIFNLYFTQK
                     VNSSTLFLLVILYNLLAFGFQPFIGLLTDKYKCPRESAVIGSILVGLSAIFVLFSPII
                     SIILAGIGNAFFHIGGGTITLNLDNRKATMPGIFVAPGALGLFVGTLIGKIGNFSALP
                     FVICLLVACFLMYIIKTPKIDYDTSKTNTNYLELILLLLLLTIALRSIVGLAIIFPWK
                     STLSLLVALTFGVVLGKALGGYLGDKFGWKRVAIISLLVSAPLLFFGVNYPLLGILGM
                     FLFNMTMPITLVAISNTLPGRPGFAFGLTCLALEIGAIFTFTSLKSTLSNNFVILAMV
                     LISTVVIYFGLNYYNKANKK"
     gene            12984..13178
                     /locus_tag="PLK55_02110"
     CDS             12984..13178
                     /locus_tag="PLK55_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35753.1"
                     /translation="MFECINKKIKKYTWRQIACIKLSVLFFTLMIAKLWPGILGLNWY
                     VYMILAIIFMIRPMIMLLKK"
     gene            13248..13508
                     /locus_tag="PLK55_02115"
     CDS             13248..13508
                     /locus_tag="PLK55_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35754.1"
                     /translation="MLKKKQDSDKILQETIYGTGGIPKEEPGAVTVGKSKRRRNEQNF
                     FQKIVSPRKIVREEHKEKKPGFFSNLFGPKFKRRKGKGLDGF"
     gene            13509..13880
                     /locus_tag="PLK55_02120"
     CDS             13509..13880
                     /locus_tag="PLK55_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35755.1"
                     /translation="MTKINKFIYWTPRILSILFILFLALFSLDVITPEASLSEIIIGL
                     FMHNIPVFILTAILIISWKKEIVGGVIFCLAGLLYLVNVVITIIRTPPFVWQYIIPIL
                     LISGPAFLIGILFLIGWFQKK"
     gene            complement(13884..15572)
                     /locus_tag="PLK55_02125"
     CDS             complement(13884..15572)
                     /locus_tag="PLK55_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOZ35756.1"
                     /translation="MNKKRNTIFAIIITIIIIGVFIFASVYSINKTPTRADLINEQLR
                     AQNASWVAKDYSDEILNIPLGYEFPEYNTNDKQIFNIDNVNEFFPENQNTDDINSQKK
                     EKTGNTGFFLLTDAVISPMVYEYNDEINWKEYITPGKEQISCGSCKAFSILGAMEGYI
                     NRKLNYTGSKRINLSEQYLISYSSFMGCGGEISMYYINLLTLAGYSPQNINNYFKEKI
                     NTELNNSNKLNSKRKLITKTQLSLAVGEVPLGVPLEEQLKYVSYDSCEIIKLEKSKTP
                     KSGMNISPISELFTSSEINSKLCPDGSDNDSLDSPVRYKYTVPKKQENLTKYFIENYI
                     EIENTDCKKKNSKTVKNIKEALKHTPVAISMAFYDSMMFTGHGVWEKLNSEKNTEPMG
                     HALFLVGWGTEKIRNKEYWIIKNSFGDHWGDGGYFRVWIGDQDTEFECREMFGFSGNV
                     ITKSGNEINELLPAVINKRKQDKLEQEKREKSLQKQQDKVDAYQEIQNNNPSYTPIIP
                     EIGTDLLNDYFQKNISYSDIESITNSDCLTITACLYNKLNNFFEITETPDNRLT"
     gene            15742..15823
                     /locus_tag="PLK55_02130"
     tRNA            15742..15823
                     /locus_tag="PLK55_02130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15776..15778,aa:Leu,seq:caa)
     gene            15846..16424
                     /locus_tag="PLK55_02135"
     CDS             15846..16424
                     /locus_tag="PLK55_02135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35757.1"
                     /translation="MKFESVILNNKEDVITFLKREELINQLYELEYTNNLIQYKKEKY
                     IKTLKENLYYGTQNSRENGVYINLLHENNKIIGFSIANKEDKERAYIGRIAIRKEYQA
                     KGLGKKLLINTVAKLRTRGFSEIELTAKPALHRLLKQLTAQEKINGKRRQYNSDFKYS
                     MSTRKLDPQKNEPVTIEIKKKKRPIIKPKLKI"
     gene            complement(16439..16825)
                     /locus_tag="PLK55_02140"
     CDS             complement(16439..16825)
                     /locus_tag="PLK55_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35758.1"
                     /translation="MDGFLTNSNITEPEIKDDSDIKNTKKIKEIPKKTETPIKAEPVI
                     IKKIKEPKAPRKPNFFRALLIGLLLFGLLAGSFVAYDKHQKGELHLENISFLKFLDMN
                     NSTPIDTNRNIMPQNNNVNLPDSNSD"
     gene            complement(16838..17707)
                     /locus_tag="PLK55_02145"
     CDS             complement(16838..17707)
                     /locus_tag="PLK55_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOZ35759.1"
                     /translation="MNFKKILPIFIFLIILNYNFAQTTDYQQTEINHYEFDTYKIKDN
                     LILSDVNQSQLVFDIVPCYDLIVKINNQKTDSISYNLNSLTINLNDNIKNLEKMNIEI
                     IYLTDYYTSKSNGLWTLIYNSKYKETIDELKITLPKSAKVNELPLISSAEVINGQMVV
                     SLFDIKNISINYELSSKTSENNYLIILVILLAIVFGIHLILKKKPKKKTKIKVSEKED
                     LLLGLNENEQKIIKVIMENEGQTQKTITAKSFLPKGTVSRNIKKLSDKGYIEIKKYGV
                     SNKIFLGEVFKKK"
     gene            complement(17782..19629)
                     /locus_tag="PLK55_02150"
     CDS             complement(17782..19629)
                     /locus_tag="PLK55_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35760.1"
                     /translation="MKIKKTINFIVAIFAITIFLIATSPRLFAEYYSIYEGQSREIKF
                     NNLNSPVTITGAYKELIDLSTGKVLAQSPIYDNSENPIYLNPNNEYRINIYFTASKDI
                     SNIADIGSSLVVNAINSQNTLMPLGAYYNHNEVIKDLKLSITESESGQEKYKYTYSYI
                     IDSEVFKEIERVSSTYNEKPKNYTLTIGEWAKINSRNIQTTVYKVGDAFDIDGYTLTI
                     KEIKANTTPPGLKHEVTISISGNNLNAIETYESGDNIFENYLNTNLKVNTVYSSRITL
                     DKETLSTTDNSDYISFSNYADIAVKITDKPLPQTDSKYTYKLEIPCAIDKTKIILPAK
                     IDPNYTGQYKTICNSSSYFNAETKTHHLKKNEGYYLDLTILTEKNNFSEIETATINFY
                     NEYNQNACFTRKLFKSSTKENLFVGKISYVKGNDSKEFALDNMDGFVNSCGIDTPLKF
                     EVTIDSKTGEKLTGINSGIIRYLIVDSDNSNLIAENARAQKQIQVKNKNLNSEVTDIN
                     YENIESINTNSTLETKSLNSKQLTVKNSDEIYVTKDNKVKKIMPVTQMLQENKIDISK
                     VVGDINLETVSEIPTYVFEVKEQRKLLWFIPIGEKITEKRISATKEIIE"
     gene            19986..>23424
                     /locus_tag="PLK55_02155"
     CDS             19986..>23424
                     /locus_tag="PLK55_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35761.1"
                     /translation="MKYSKIILICIIFLILPFIFAEEESASVVVPLAINGIEGELDIS
                     TTPLIFPETEQDESSDTINIFTSIDNNLGFSLNTQLNYKTLDIPYYFLKENQNLKKRI
                     YPFNQGRTYVINNSSQEITKDKTLELKLNLSDTTQTVYLVIDNLQSSQIPEMNRFVNV
                     TSSTIEINSVPRYLKINKSAIANNKLLLNLKANITNFSFQIIETDNNKNVNGMYSTNI
                     LQLDYDSFNKYALFQINRNIIEFYCPYCHLKDYGINENGFSACGKSVFEEEKKPITEY
                     LSAKENVCLLPDGSKISGFTNDSLEVFGLDRLYFEWNDKIPSDLFDYGEYYVDQDQFR
                     IAVSKKIDAITNNNYIEIGNIKYKKGKNNLISKAGVYIEQDLSKITDLSTNNSIIESL
                     EFSIAVLNSIPEEYRKLTILDLTNNEPDVSDSEFNRLLQEILGGNYYKFTDYHTHHQI
                     TLEKYLSSINNFKVSRVNNDIYSKLLYGLEYKTQGYSNIYYSEGLNYFIYNIKPFTEE
                     TTGILNNEHTQMFGNYWKLNLIDLNNTIEPKTVVVNVSEITPESKRAKIIFNNPSTNL
                     YVNYYFENQYFSSNPLFLKTINALSYNRGNIFENTSSFTSKLPEVIQNYNNLQDGYIV
                     SIDSVHSDFIINLTKPVIITKGAGEEILVKYYNGREYVNYTNNLTTINNSREDFATKE
                     TLVIYPPKSENTNYPIIIESQSPLIFNSEVFESTENGKHIYRLNKENQNLRFLELIND
                     VYNNEACFSISENQFSLWKNIDEELDIKKAESYLEFASALQYNVGESLENQISQNQNS
                     QPNNSFAVYTIDESQTLYKNAQAVNTFIKTETDNRNLNILKDYSEVYEKAIESEPTLY
                     KEFRDLLNCNETLICRISKTRYTPQGYFFERFKNVCVDNTPTDCVSFWSNPKVNYCSA
                     FVRNFNNNFFGYRFDSADAWDLAKQPNNKSIWKATTDSLQEKDFDLLIPGSVLGIKHN
                     NTSYKNKEYSHVVVYLGKIGSRHYIIHSWVNTLKIERLDVFLKTTARGKNAYGYYENG
                     QIKEVMISNNLNQKLIAKARENGISLGSVDSSIYQGIIPDYIFDSFNNNKMLLTNIEN
                     PSNNAVYQELEEVYNRILQKDFDIYYKTNYYTEESPEINEKIRLDSNEPMIVILGKYK
                     "
     assembly_gap    23425..23524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23525..24826
                     /locus_tag="PLK55_02160"
     CDS             <23525..24826
                     /locus_tag="PLK55_02160"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOZ35762.1"
                     /translation="KIRLDSNEPMIVILGKYKRLFLVKKENNRTNIISEYPLSTGVNG
                     FGCETDSLKTPMGLFKIVQKVGQNCSPFQIIGASGCQYSDGHPVIASKNTGTAYVVTR
                     KLVIDGLEKENIGLGCDNGNRNTIYRGIYIHGTNKENSMGQQRSHGCIRMLNNDVISL
                     FDQIENGTYVYVYNSEISYSNLLDLENQFASNGTSLNQFVFFSWFNLFFPPVIEPTRP
                     KPKTKEEIINSLAKGTGNCYTTNGNTLTTLRSGDLGLTTKVNTRRTKNSVVVPSTHSV
                     SLSDIYTIAKDRPNNYRADNKFLTAIPSLYNLCAIYGVDPIFILAFFKREKSMYNMLA
                     GNYNNGELTATNNPSGIKATNGCACGGSKARNTEFCVYPTLDAGFEATLINLKNNYSG
                     RVLSTGSNKNRGVAEKWNPGGTPSYGRDLVNFINQFYGYIN"
     gene            complement(24819..25487)
                     /locus_tag="PLK55_02165"
     CDS             complement(24819..25487)
                     /locus_tag="PLK55_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOZ35763.1"
                     /translation="MNEIKEIYNTLFINYGPQGWWPLSDIGYHPGKYFLIKTHKQIFE
                     VYVGAILTQANSWTNVEKAIFNIKKNDLLNPKAIIESDSRTIKHAINNLSYYNQKYER
                     LVDISKFYLTLKDRMPTRHELLSVKGIGKETADSILLYAHRDPIFVVDTYTRRILLNL
                     KLIKKNAKYEEIQKLFMDNLPRDFKVYQEYHALLVEHGKRYYSTKAKKGKTLKIRDPL
                     LKFS"
     gene            25598..35092
                     /locus_tag="PLK55_02170"
     CDS             25598..35092
                     /locus_tag="PLK55_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35764.1"
                     /translation="MNRKFLVFITIFAIVLSLNLIYASTLSVDKNETYLGDTVVITGT
                     GFSPSSTLVLQNNLEPNSAWWDGYNEQWGFPTFVTTDESGNFTYNWTVPFDFRISPGK
                     QYFSVGNNISATIYWNGGGDYPTSKNKTICDGNYCYVTSTYTNPNGGVEKFNMSTGAR
                     ECNASVAVTGTGIAIDGTYIYTTYAATSNNLRRTAMADCTGTSTFTTAGSNTYSVAIS
                     NGYAFFGYNVNIAPRKYENLSTTPSQVVAFAPASRNSIANYICVTPDATKVVVGYADG
                     NVLAFDYTTGATLWNSDPDDDVTQAISGMHCDNDKIYVAKAYNSTTNGPLFGGFAYAL
                     SASDGALVWRSERIDSHYVYSSYTNVACDSVNCYASVLSPPLGGAGINGAPIIQIDKT
                     TGEIGWVKWRSWLPASTGDYGTSTTHQSPMDLWVDSDENGFIYPVYSGGRIQKLSKND
                     VSVQSFPDTNLWIRSNTNLYNFVMNPTKNVFYSNERPSFNFKAIDNYGQYMDADDNRF
                     NTSHLKFSTINAYNEFTAAEAGSATVASQFMDPRIMSVYSATATLPNPTPYNYLKFMY
                     DEYDSGVFCETAVYNTKLFGWHINNRYCLYENLEVNNATLNHTQNGVKLASIANYFLV
                     NPNKWNNFPITKTFGTDNWTWSSSHNPTYDQILGYATRFGSDFNEAWFNQLGQGTSFN
                     TNTGTTSLIDYIDATQDFNNADPRLQKWHESYFLFKPLGSGADGNRHWQYIENDTNMI
                     ATPITVVQGTNQSGSYLIGADTFSNRIDFNATEAGILERTNEPFWFDVNFIDSINSDC
                     SDIVVTDITDTPIGLWQLDSETCTANNYYTLFVQDNFTLGQEKQYRLYYNSPTAAVTP
                     GGTTDLITSLVSGTCAAGTDIIDVNSTNWYRWVQPGVGAVAANQLYFNFGGSSNTWFN
                     HATTRYALRAGYLNSQSAGTGAGSVTNTLGTALATFTSRCYQRSYNKDKIFTRIDMNT
                     ANFTIAPHTPTEINPNATTTFTRKIMSRWYFFNGNGRIRIMFKLDEPLPVMTTGISQT
                     YHPYFYFGMYALVPQGTATVAANNFVNWSGRKELMDEYTPVQLSPDKKSAILTPSPLP
                     LTFTIDYNGEHFPTVSSASSKVGGGCTVHKVWPYFSWRPIDPVTTTNATTFASAYTTD
                     FISCDEDLNGTIPDIGNDGYVNVFPKESIYGTGLNRFVDPNYIAKFYLKPNLYIPDNR
                     ENRWLPPLALNSQYGTSAKVSTIILDVCPTEEYGYKGYDDFLADLDDLISSSSIHKVE
                     ENTTNIPCATSITIFSTNTGSIGKYFLFQRDVNVVVADVSRPDAPYPSYLYDPTTGAL
                     SINGSLDYTNAQKSTQYLGKWFFTSSSDPYVISGYATTGAEQLQGAKRYAWAPSFAFN
                     KKVYSENLFWNKSMSNLTYQQSPCAWNFSTNYWGDMDFGPGWKKTMSTTQQGTICPRN
                     ESNMEHYSSFRMALASLYPANALVVTAIDNVIDGRPNKVTTINDLGIDASMFSRHNSL
                     WDYNHYSDYTQVLSDFSELTEGTKHSNDEPWQCDFSRYDSKNFCIVPATTTTLIYTAT
                     PGVLTASTTSGMWSPYYQSNREYSVLRNYFNNVWFNTAAMFPTYDFLTENTIFPMLGE
                     TEFTYDTLYTSNNPNDYNDLMDKLFPRLHGALYQNQFLLRDWKNTAFMVLSGGDTREY
                     LRGTTINVSGFMMKDSDSLANAPIIVQVFKNSAPESPLTTYQTITDANGDFNYSYTIT
                     PTAEPGLYFFKAIYNNGEITGTTSFKVSALNIATVLDKSVYVAGDSVFVTTTISDAII
                     GDLISDATVNLVISGPAGNTLLTTPMTNAGFGVYTYSFQLSPETVNGTYVVTITATTA
                     DNTGSATTTFGVGVAVSSINIINNFQISDANIEYVTQVTTLQNVVATPLLEFTINYQL
                     SGGLDLENITLFTKDDIVLERVYDFDDLNRTTYYLSLDSSNNPIVYIMANFEASQIRT
                     MTFIAENKYYEHIEQDYNSVNNYSSELSSYCSILLTPAAQADCANANTYLIDINDYYT
                     QAQTASSIDQFYNIKIAIGEKYILFMDILDSLRILATNQASCQIFVPGTWSLDSNVSF
                     AVLMNGPSSIPINGLTFDVNLFNYNSVLVDSYSVDEYGEGWYYSSFSTPSTGGGYNLR
                     IDQNIGTNRFQCAASFDVNQASGSVIGGEIGGGLTADQNATLYEILGIVTDINATLND
                     LEEIIFDINAIVTDINSTVSDIDSRLSTLETSLSDINLLLTGIDSNINYIIGNMATTT
                     DITNLDSTLQTILGYNVDINQTVHDSATQLNNLYDLLDDIDDQIDAMNNTITDINALI
                     TTCENAPETSICADINGLSNQLNNINSTIDIISSTTQDINSTTSSMQGTINTLNNTSN
                     SILSTVTDINNTSTFILSKVNTIESNTQDIQSNITTIMSDLNCAGTPPEYSVCGNLLN
                     ISSLISDFNISTQLSLLDISNALSTIEYNQQDINQSISYILEDLNCEDENASPICSSI
                     QTIIDNTDTLETSISDLNNTMIIIGDNVLDINSNTNNVLLGISDLNSSNQEILDDLLN
                     ISEEITNTQGLISDTNLNLTNYLYDINDTVDDINDTVDDIGGTTGGTSDKVDEIKDIV
                     LNIDNNVSEINTTLTDINTQQQAIQATLASMQLTLSDINDTTTNIYTDQQSRFTVAIS
                     DFSEIGSGDVYRAKVWVFDYDGSPKDADSNDAKITLYDPERNIITENITMTRLSEGVY
                     TYDYNVGTGQIAGIWEAVATVLVNGENVSPSDFWELVGNPPEVRINSIVDTSIPTITA
                     DVIITNEGSASQEYQYEYCIVSDQSNMCGGGDDVDYSSGAKLILPGQTWNTELTLNVP
                     TVGTYWFKIKVYYGVEMSAASKQFVATTGGTNTGGTGGGSSDISDVTGLVSLPVAVGE
                     IAIIEYPTDMTIIQGDYGFYTLRIKNIGSIDLSDIKLTIEDLPESWWEIQQEKTILSP
                     NEEGSFVIKLKAPMDAEVKDTLVKFKISSKEDTRLRESILRVLEKSLAEIRFVDVKVS
                     PIKVNGYGEIEFIIQNMSLYDQNITATLLVPRDWQIEDSDQAIILKGGETKRIIFVVN
                     ASYISGVRDLVFVIKNNTGQRFKTGEDKLFKQLMVIVINDEKVKSELNFFNQFNGIGI
                     GILIIILLLVILIIIFLFGIKNNKKQRPSLGYNRNILDKLMGLRRK"
     gene            35089..35424
                     /locus_tag="PLK55_02175"
     CDS             35089..35424
                     /locus_tag="PLK55_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35765.1"
                     /translation="MISGSLMFVLFINFVIIMCVVIFFLRHMSLRFREEFDETNKRLE
                     MIEHNMQHNELRLKHLELRIDKHHDVSEHHYKKPEIHKDIEDMKIVVKKINQNTEKIY
                     KKTEELFKK"
BASE COUNT        12481 a         5090 c         5316 g        12432 t
ORIGIN      
        1 aagaaagcag atattggcat agctatggga attaaaggca cagaggccac aaaagaggtt
       61 gctgacgttg ttctaaaaga cgataattat tcaagtatta tttctgcaat taaagagggc
      121 agaagaatat atgataatat tttgttgttt gtcaagtaca tgctagcagt taattttgat
      181 ttattaatta ttgtagcttt acttacaatt ttggcttttc ctataccact acttgcgtta
      241 cagattcttt ggattaatct tgtaactgat tctttaccgg ctattgctct tggtagaaaa
      301 cctgcaacaa aagatataat gttaagaaaa ccaaacaagc acaccggtaa ttctttcaag
      361 tcttttacaa cttttataat tattgcttta gttgttaaaa ttataggtga aataattttg
      421 ttttcttatg gtcttaatat tgatacaagt atgggtatca atccttttga tattagtcag
      481 ccatcttatg caaggacttt gttattaacg ggcattgtag tttttgaact aatatttgca
      541 tttgcttgta attcagaagg gtcctttagc ccaaagagat tattttctaa caagatgctt
      601 ataggaagcg ttattttggt tttagcttta caattatttt taatatacaa tccatttatg
      661 caaaatgctt ttaatgcagt ggcattatct tttaaggatt ggttaatggt tttagcattt
      721 ggattgtttt cttttataat tattccaatt actaattttt tagaaaaaat aagatcaaaa
      781 agttaatttc tttttatttc ttttctaaaa tagaaaagat atcttaagaa aagaatagag
      841 tacttaaaga ttttaacgct tgtttctttt tgttctttcc aagtaacagg atagtcagta
      901 attttaaggc ctttatattt tgctaaaacc agaagctctg tgtcaaagaa catattgttt
      961 ctgtctcctg atagtctttt tttaagaaat agctctttta tctcataata tgctctctta
     1021 ttaattgcct taaagccaca ctgtgtatct gtaaagcttg tacccaaaag catcttttgc
     1081 agaaaaagat atcctttgct tataatatat ctccctattg ttcttttggt tttagattct
     1141 ttatggtatc tactgccata aaccaaatca tagttttctt tagtaatttt ttttatcaac
     1201 ccaggaaaag catctattcc ggttgcaagg tctgcatcca taaatgagag aatatcgtag
     1261 ttctttccag tattatgcca ggaatacttg attgcaatgc cctttccttg ttctttaaca
     1321 aaagtgtact tgacagtaga gtatttcttt tctagttttt ttgcaatatc ttttgtgttg
     1381 tctttagagt tattatctgc aatatcaatc aggtatggtt ctttaagttt tttagaaaga
     1441 aaactattaa gtctagttat gtttttttct agtttcttgt cttcgttata gcaaggtatt
     1501 gttattaata ttttcgtgtt tttcaatttt ttcacaattt atttttattt ttctgagtta
     1561 ataaaataac agatattttc tattggcttt attttattaa ataaaatatt acagggatta
     1621 gtatataatg aattatggat ataaaatagt tctacatagt tttcttttag tttacaattt
     1681 tttaaatctc ctagtatcat gatgttgttt gttttaatta ttgtgggcaa ttgattttct
     1741 aatgctatta tttgttcttt tgaacgagta ggagtatata gaaacaaaac tttatcctga
     1801 taatttggaa tatctttaat atatatttct attggtaaca taataatatt agaatccaaa
     1861 taattataga atgtccaggc atcagagtac attacacaat cttctaaatt atttttatag
     1921 attgtgttta tggcattttt atatctagta atatatggat aatctaattt ataggtttct
     1981 tctaatattt taatagaact aataagcaca gaaaaaatta gtagaatata tatcgttatt
     2041 aaataaatgc gatgtaattt aattttgtgt tttttaattt tttcagcaat tgatgttata
     2101 ccaacatatg taaaataacc aattggaagc attaatgtga aaagatatct catgtcttta
     2161 aagggggttg aaaaataggt gtgtattgta tatattgtta ataaaagcat tattatagaa
     2221 aaggaattat ttagaggttt agcgttttta ttttttttaa tcaatttata aagaatatat
     2281 aataggccaa ttaatgtaaa tacaaataaa attgattgga cactaataaa atgaataagt
     2341 tttggagctt gtgcaatatc taatctagaa gtaatgtttt gatgatattg gtctgctata
     2401 cttgtaaaga aattaccaaa ttttagtttg ctgtaaataa accaaggtat aaaagttaag
     2461 caaaataaga ttatagattt aatttttttg tctattcctt tatgaaacag taaaataacg
     2521 ccaaaaacaa ttccagtata tctggttaat gcagaaaaac caataaataa cccagagatc
     2581 caattatttt ttataataaa atatatccct aataataata gggctactga aagtaattct
     2641 gtgccgttta tttgtccaaa ataaagaacg aacggagtaa ataatattag atagaataat
     2701 tctgtattaa aatttaattg tttagatatt aatataatac ttaaacagaa tagtgcactt
     2761 acaattataa tatatattat ttctgcagca gcatatccta aaatttgtgc aaataatcca
     2821 ataataaaag aagttagtgg gggtctaaat gtttcaaaat aaagtccgga attaaacatg
     2881 ttttttgcat ttaaaacata tgatataaaa tcccaagaaa ttgtaaattg atgttgaatt
     2941 ataaagaaaa gtgttgtaaa taaaaatata aataatacaa atttatattt ttttaataag
     3001 cccattatcg tttttgaatt cattttattt taggcacccg ttttattcaa gtaatatcat
     3061 caacagcata atcccaattc caagaagtaa tccaataagc tgaatgcttg tttttttaag
     3121 accaatgtct tttttcattt ctggtattag atctgctgtt gcgatgtaaa taaatccacc
     3181 tgctgtaaaa gcaatcatta ttcctgcaaa agaagtaatg cttttgctta atagcaggcc
     3241 aacaatgcct ccaaatattg cagtaagaga aattaaaaaa ttcattaata atgctttctt
     3301 tttagaataa ccagcataaa ttaatactcc aaaatctcca acttcttgag gaatctcatg
     3361 caaaaggaca gcaatagttg ttgcaatacc aagaggaata ctaacaacat agcttgttgc
     3421 tatgattatt ccatctaaaa agttatgaaa agcatccccc agaaggttca ttgcagctaa
     3481 agggtgtgga tgttctttag aggttggctg atggcaatgt ctccaatgta ttattttttc
     3541 taaaataaag aaagctaaga ttcctgaaat tattgctaaa ggtataaagt taatgcctga
     3601 ttccatggcc tcaggtaaaa gatgtaaaaa tgaatctcct agtaaagcac cagcagaaaa
     3661 actaattaaa aaaagcaaaa caattggtaa atgcttgtct tttgtaaaga ataaagttat
     3721 tccaataatt gaaataatac ttacaacaaa tatgctaatt aatgtataaa tatatataga
     3781 catcatataa taattatcaa gaatacaatt tataaacgct tttgaaatat agaatatatg
     3841 tgaggtaata ataaaatgcc aagaaaattt ggaaaaataa gagaaccaat aattagtata
     3901 aaacgaataa aaagaggcgt tccagaaaaa gaagaatggc caatgtcttt tggtaaaaaa
     3961 gaaagattta ttagtaatcc tctatattta gacacggttg taaatattaa tacaaaaaat
     4021 aaatccccta aaattttgat aatgggtcca ggacttggcg aagaaataat agagtttgat
     4081 agctttttaa aaacacacaa acatattaat ccacaaattg aatcactagg aataaccaat
     4141 caactaacaa aagatgctct aaaaacagta aaaaaagatc attctcttgg tattgcttta
     4201 gaagagattg atccagaaaa ccccatacat aagcaagcta taaaaaaatt aattgggaga
     4261 tttgattata taattgcgcc agttagtgtt ggtattcaca caagatatcc tgcatataat
     4321 tgttttttgt gtgccttagc attaaaaccg caaggagaag ccagaatcaa tattatgaat
     4381 ccagaatcta taaagagcca tatggatctt tacaaacacc aactaacaga aacttcagaa
     4441 caatatactc ccacagaaat aagaaacatt actgcaaatt ttaagcttgc aaaattaaat
     4501 tataatcaag taagtaaaat agaaaaaata atatatagaa tgttaaactc ttattttaaa
     4561 aaagatgtaa aagaccagtt ttttattaaa ataagaacag attctagttc caaacaccag
     4621 ataatagtaa taaaaaggaa ttattaaagt gataaattat gcccgaaacc ataaaagtaa
     4681 attataaaca agtagagaat ttttgtttag aaatatttaa agacatttca aaaacaaaat
     4741 ttcaaccaga ttgtgtaatt ggtgtttctg ttgggggggt ctttccggca atccattttg
     4801 ccagattgtt taatactaaa aaccttctaa caattgcagt aaagtcctat gatggcaaaa
     4861 aaagaaaaga aatacaagtg attaatcttc caaaaaaaga atctcttcta ggaaagaaag
     4921 tgcttttgat tgatgatatc tccgatagtg ggagcactct aaaatttatt gtaaatcttt
     4981 taaagaaaga atataaggtt aaagaaataa aaaccacaac aatatttgta aacgaagaac
     5041 attgtaaata ctatccagac ttctattcaa aaaaagtaaa taaatggcta gactttccat
     5101 gggatagatt tgaaaaaaag taaccattgc gcgctaacca gagcgaaacc ggctaagctc
     5161 ctagtgggat ttgaacccac gacctccggt ttacaagacc agcgctctac cactgagcta
     5221 tagaagcaag aaataagtcc ttttgtctat ataatatttt atttaagaaa tatttataaa
     5281 tggattttat tgctgcctta aagcatcaac aatattcatc tttgctgcag ctctagcagg
     5341 gaaaagccca gataccaaac caacaatcaa cgataataac aatgtcataa taactaacca
     5401 aggagtaatg tgtattgtaa gcattgtaaa gttatgtgtt gctgcataaa gtattacaag
     5461 tcttgcaatt ccaattcctg caaaaaagcc aataattcct ccgacaacag ttaacaccac
     5521 agattcaaat acaaatataa aagtgatatc tgaaattctt gcaccaattg attttaaaac
     5581 cccaatctct tttgtcctct caagaacaga agtaaataca gagttagcaa taccaagaga
     5641 cgcgacaatt aatgaaattc ctgcaattgc aataagaatt gatctcatca tattcataac
     5701 tcttctgatt agttctaaaa aagattcaga acttataact gtatatgtgt cctcttctct
     5761 tgttttttta agtattcttt ctattttttt aactaccaag tcagcatctg catcttcttt
     5821 ggatttaatc atcatgtagt ttacagtttc tccataatca tcaacagcaa gtcctgcttt
     5881 tcttccaata tatatagaac tatcgtctga ttcatttcca actgcttcta atatgccgcc
     5941 aactttaaaa tcaacatcat taatcttaat tgtatctcca acttttattt ctctattaaa
     6001 tgcttctttt gcaaggtatg gtccaataat tgcaatatat ccgtcttttc catcaaagct
     6061 ttttccttta tctattctaa catcaaaaaa cttaaacata gagtccagtt tatcagaagg
     6121 gatagtaact acataaacaa aaagctcttc tcctttatat tctcctttag ctgtaaagaa
     6181 attcattgca tatacttctt caacttctgc aatcttagaa atactatatg cttcgctcat
     6241 ctttaaccta ttttcttctg atactcttcc agaagtattt ggattgccat taaatgcagg
     6301 catcacaaaa atagaatttg cacccatctt attaaattgg tcagtgactg ctgcaagcat
     6361 tccttcgctt aaaacaatta atgcaacaac tgaagcaaca cctatgatta cagaaagaat
     6421 agttaaggtt gacctcaact tctttctgaa tatattcttt atggtgagat taatcaacga
     6481 tttatatttt tctgaactca tttatttcct ttttttaaag acattttgat ataatcctcc
     6541 aaaagctgga atcaaaatct ttttgtttgc ccatctaagg aataatatga ttaaggcaag
     6601 aataattatc ccaattaaaa ttccaataac tatacttatt atccctgaca ttacagattt
     6661 tgttttagga ttttttagaa tttcaacagt tatgcctttt tttatttctt gttctttaag
     6721 agctgagtct ttatagtgta ttgtaatctc gacaggatag ctccctggct taatgtctgc
     6781 gtttgtatta aattctaaag ccacagaatc ataattgtct ttctcaatag aaccaacaaa
     6841 gtattttgga gtttcagttg ttattgtgct agtcatttct gcataaactg attctgcatc
     6901 aatgttacca acattataga tttctagatc aatcttttct ttgtttccaa tatagatttt
     6961 gttattgttg tttgttccta tacttactat atttaaaaca agcttagggt tatcatcaat
     7021 ttttacacca atatctcttg ttattgtttc ttctcctgat tgcccagtaa atctaagagt
     7081 taatggcaaa ggatagctat tttgtgtaat tgtttttgca acatttaatt tatattttgc
     7141 tttaatttct tgtcctgcac caatattttc aaaatattgt gttttaagat taagaacaga
     7201 aatatagtct cctgtagagt cttctaaagt tacaaaaaca tctctaacag tctttgctcc
     7261 tagatttttt atagttaatt caagttctcc gtctgagccg atagacattt cagataattt
     7321 tgtgtctgta atttcaagaa cagtagagcc tgaagaaaca tctattgaat aattttctat
     7381 gttatatact tggctttgtc caggtttata tttaaattct atttcatata tattgtcttg
     7441 tgctttaata tctgttgtta aattatattt gataattgtt gtttggtatg gtgccaacat
     7501 ggctattgtt ttttctgtgt tattaaagtc tgcaacagaa aacggatatt ctaagtctag
     7561 tatataggtt acattttctg caagaacgtt actattgttt gttaactgta tccatagttc
     7621 aaattcttct cctggagaaa caggaactgg actatagttg tatgtatctg caaccaaatt
     7681 tattgaatcg tagtctgttg ttgctgcata tatcccatta agaagtataa tggaaaacag
     7741 cagagtcatt atatattttg ttttcatttt ttatctcacc attttatttt ttataagtat
     7801 tgcgcgcgag ccgagcgaaa ccggctctat tcttctcgaa gagcatcaac tggttcttga
     7861 tttgaagcaa gatatgctgg tatcattcca gagattactc caatcataaa ggaaaagcca
     7921 agtgctaata tgattatgct tgcagttatt gaaatactta ttgttattcc tacttgataa
     7981 gcaactaatt ttactagtcc tgctaagcta aatcctaaaa taacaccaat ggtccctcca
     8041 agtaaaccaa tgattccgga ttctaaaaga aatatcatta agatgtctct tctttttgca
     8101 ccaatcgctt ttaaaactcc aatttctttt tttctttcaa atacagaagt atacattgag
     8161 ttaagtattc caactcctcc aactaaaagc gaaatacaag caataccaac aacaacaatt
     8221 gtaacaatac caagagtttc ttctctgttt tttgcttctt gtattgggga agtcacagta
     8281 acgtctttag catctcttgc tttttctagt tttatttcta ttctgtttgc tgcattctct
     8341 atgtcttttc ctggttttac aattgcaaag attgtgtcat aggtttctga ttctgtaatt
     8401 tcttgtaaat tctcatttga aagatacatt acgttgtctt ttctagagtc tccggttttt
     8461 ttaagtattc caatgactgt ataatcagag gttgcaattt ttattttttt tccaatatct
     8521 agggtttttc caaaaaggtc ttttgctttt tctgtatctg tatagttgta accaataatt
     8581 actgctttac ttttttcatc agtaatgtat tccccttttt caatttctag attattttgt
     8641 tctaacattt cctgtaaata ttctgcagca taagaagtat atgaaatcat tatagtttct
     8701 tgagcatatt ttgtttcaat atatccattt agagagccac tactaaattc aatatcacta
     8761 atcttattta tagtttttat atcttcatca gttagtcctt tcccactgct gcttcctgca
     8821 aaaccagcat atttagaagt tattgttatt ttgttagctc caagactatt aaattcttca
     8881 tttattgtat ttttaagtcc ttctgaaaga gatattaaag aaacaattgc aattactcca
     8941 acaacaattc caagtatagt tagccaattt cgaagttttc ttcgtctgat atttctaatt
     9001 acgaaattaa atattccaat cattttaatg cgtaatttta tcttctatga tttttccatc
     9061 ttttagatgt atttgtcttc ttgcaagatg tgcaacagtt ctatcgtgtg taattaatat
     9121 tatggttttt cctttcttgt ttaaatcttt taaaagatcc ataatctctt cgcttgcttt
     9181 agagtctaga gctccagttg gttcatctgc aagtataatt tcaggatcat tagcaagtgc
     9241 tctagctata gctactcttt gttgttgtcc tccagaaagt tcagttggtc tgtgtgtgat
     9301 tctttctcct aaacccacaa gtgttaataa agctttacaa tgtttttctt gaatttctaa
     9361 aggtttattg ttttcttgaa aaagcattgg tagcattaca ttttctaaag cagttaaagt
     9421 tggaataaga ttaaaatcct gaaaaacaaa accaatcttt tgtcctctaa tctttgcaag
     9481 attgtcttcc gaaagattag aaacatctgt tccatcaaga aatacttgcc ctttagaaac
     9541 taaatctagt gctccgataa tatgcatcat tgtggattta ccagaaccgc ttgggcccat
     9601 gattgctaaa aattctcctg gtttaatttt taggtcaaca ccatttaatg cattaacgca
     9661 agcttcagaa cccatacagt atatcttata tactcctttt agttctacaa catttttctc
     9721 ttttatagcc atagttattc aaccaatgta aaaaatcgca agtatactag taatatatat
     9781 tattgtacag aaatatatat aaatccataa aaacttccta aaatatagct ttatatatat
     9841 ttttaatcct aaatatatta atcgaccaca gatattaaaa aatagaaatt ttgcagtttc
     9901 ttttccaata ggttgattat tgggtgcgag ctgaccnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnntgca gaggtggcgg agtggtcaaa cgcgctagcc tgctaagcta
    10081 gagtcgaaag actacatggg ttcaaatccc atcctctgcg ctttttattc aagtaagcat
    10141 tttgttgtaa gtgagcttgt agagtggatt aaagggaagg actttgctca cgactacgag
    10201 acaggactaa tttctaatat ttctagatta ttgttgaata acgataaaag tatatatttt
    10261 tatttttcta agtgtatcta ttgtatgttg tgtatggtta aatgggatag caagatattt
    10321 aaagagtttt agtcagttaa taatatttcg tggtggtaat ttgaaacaca accaaagaaa
    10381 agacaaattt aatagattct ataaaagcaa ttggtttatt atctgggttg tgatttatgg
    10441 aatattatat tatttatcta cattattaat tactgctctt ggaattagta attattacat
    10501 taaagtaata ttaataggaa tattaattag tattattgca agagcaatat ccactcatat
    10561 acacaattct aattttaagc taaattttaa tttatttatt tggacattag tatatatttt
    10621 attgttattt gggtttagat ttatattatc ttattttgcc ttaaaccaat ggttggctat
    10681 tattgtaata gcattaggat tttcaatatt tactcattta attaaaaaaa taccatttga
    10741 aattttagcg ataatttgca ttataatttc ttggatgttt gcggcgttgt tatatgtaaa
    10801 tgaaaatgga tttgatttat caaaatatat ttataccagt aattctttat ctgaaaattc
    10861 agatttaaat cgcaattcat taagattagc gtcaggtagt ggttctaaag actacacacc
    10921 acacactaac tacatacaaa ctcatcagcc tgctaagcta gagtcgaaag actacatggg
    10981 ttcaaattta attataagtt ataggccttt tatttaggtt ttttaaatat aactaattgg
    11041 ttttatgttt tcactacaag gggattataa aaccagataa aaaagaagca agattcagaa
    11101 ctatggaaag taaaagtaac tttttcttat ctttttctaa aacataaaat aatattttcc
    11161 attctacaaa aacaattgtt atttcaagaa caatagttat tgcaaaaatt acattatata
    11221 ctatttggaa attatagcaa ataagcaaaa tgtaattcaa tagcggatta gtaagtaaat
    11281 ttattaaaag aacaacgact agtgatttct tgtctttata gccaaaaaag aaagcaataa
    11341 tgcattcaat tataattgta aaaactaatg ctattaatag atttgtaata tattgtggga
    11401 tcatggtttt ttagtattta ataaggtgtt tttcttaatt tttagtatag atataacaat
    11461 aaacacaatt attgttataa caaccccagg cagaagtatc cacaaaaaaa taatctcttc
    11521 gggggtgtgt acaacaaccc tatcggcata ggtaaaattt agtaatgcaa gaattgcaga
    11581 aatcatcaaa ataaatttat attttttaat gttatcgcct tatttttttg tgagttgtgc
    11641 cttgattttt tttattagaa atatagcaac acagataatc cctacaacaa ctgccccaac
    11701 aataattatc gcagccacag ggattatata ttcaaaaggc gttggagcca aaaccacatc
    11761 tgcataaatt atatttaata aagatactag tccaattaaa actaatagca ttttattatt
    11821 catcattttt tattcgcctt attataatag tttaaaccaa agtatattac aacagtagaa
    11881 attaaaacca ttgctaaaat tacaaaatta ttagataatg tgctttttaa gcttgtaaat
    11941 gtaaagatgg cgccgatttc taaagcaaga caagttaaac caaatgcaaa tcctggtctt
    12001 cctggtaatg tgtttgagat tgccacaaga gtgattggca tagtcatatt aaataagaac
    12061 atcccaagga ttccaagcaa aggataattt actccaaaaa acaataatgg tgcagaaaca
    12121 agtaaagaga ttattgcaac tcttttccat ccaaatttat ctcctaaata accacctagt
    12181 gcttttccta agaccacgcc aaaagttaat gcaacaagca aagataatgt ggatttccaa
    12241 ggaaagatta ttgctaaacc aacaattgat cttaaggcaa ttgttaatag taatagcaat
    12301 aaaattaatt ctaaataatt ggtgttggtt ttactagtat cataatcaat cttaggagtt
    12361 ttaataatat acattaaaaa acaagcaact aaaaggcaaa ttacaaaagg caatgctgag
    12421 aaatttccaa ttttacctat aagtgttcca acaaataagc ctaaggcccc aggagccaca
    12481 aatattccag gcattgttgc ttttctattg tccagattaa gcgttattgt tcctccgcca
    12541 atatggaaaa aagcattacc aatacctgca agaattattg agataattgg cgaaaataaa
    12601 acaaaaatag cagataatcc aacaagtatt gaaccaatta ctgctgactc tctagggcac
    12661 ttatatttat cagtaagtaa tcctataaat ggttgaaaac caaaagctaa tagattatag
    12721 agaattacta ataaaaaaag tgtagaggaa ttaacttttt gtgtgaaata tagattaaaa
    12781 attactgctg cacaagttgc atcaataagg aaatgtgcca aactataaac cattaaatta
    12841 ccaaaaatat ttttcttaac aacttgcata aaatttataa gaaacataac ctttttataa
    12901 agataataat tagttagttt attttatata gaaaaccata gttttttaag cataatctcg
    12961 tttcttattt ttaggtgatt aaaatgtttg aatgtattaa taagaaaata aaaaaatata
    13021 cctggagaca aattgcttgt attaagcttt ctgttctttt ctttacctta atgattgcta
    13081 agctttggcc aggaatctta gggcttaatt ggtatgtcta tatgatatta gcaataatct
    13141 ttatgattag accaatgatt atgcttttaa aaaagtagtt tttcaaataa aatatcttta
    13201 aatatataat gtataattat attgatacaa gaaaaaaagg agtaattatg ttaaaaaaaa
    13261 aacaagattc tgataaaata ttacaagaaa ccatctatgg aactggcggc ataccaaaag
    13321 aagaaccagg agcagtaact gttgggaaaa gtaaaagaag aagaaatgaa caaaactttt
    13381 ttcaaaagat cgtgagccca agaaagatag ttagagaaga acacaaagag aaaaagccag
    13441 gcttcttttc aaatctattt ggtccaaagt ttaagagaag aaaaggcaaa ggactagatg
    13501 gtttttagat gaccaaaata aacaaattta tttattggac accaaggata ttatctattc
    13561 tttttatact ttttttagcc ctgttttctt tagatgttat tacacctgaa gctagcctta
    13621 gtgaaataat tattggttta tttatgcaca atattccggt gtttattctt acagcaatac
    13681 ttataatttc ctggaaaaaa gaaatagttg gtggcgttat tttttgtctt gcagggttac
    13741 tttatcttgt taatgttgtg ataacaataa ttagaacccc cccttttgtg tggcagtata
    13801 tcattcccat tttattgatt tccgggcctg cttttcttat tggaatttta tttttaattg
    13861 gctggtttca aaagaaatag attttaagtt aatctgttat ctggtgtttc tgtaatttca
    13921 aagaaattat ttaacttatt atataagcaa gcagtaatgg ttaggcaatc gctattagtt
    13981 atactctcaa tatcagaata acttatattt ttttggaaat aatcatttag taaatctgtg
    14041 ccaatttcag gaattattgg agtatacgat gggttattat tttgtatttc ttggtaagcg
    14101 tctactttat cttgttgttt ttgaagtgat ttttctcgtt tttcttgttc taatttatct
    14161 tgttttcttt tatttataac agctggtaat aattcattta tttcattacc tgatttagta
    14221 atcacgtttc cagaaaatcc aaacatctct ctacattcaa attcagtatc ttgatctcca
    14281 atccaaactc taaaataacc tccgtctccc cagtgatcgc caaagctatt ttttataatc
    14341 caatattctt tgtttcttat tttttctgtg ccccagccaa ccagaaatag tgcgtggccc
    14401 attggttctg tatttttttc agaattcagt ttttcccaca ccccatgacc tgtaaacatc
    14461 attgaatcat aaaaggccat agaaatagca accggcgtat gttttaatgc ctcttttata
    14521 ttttttacag tttttgaatt cttttttttg cagtcagtat tttcaatttc aatataattt
    14581 tctataaaat attttgtcaa attttcttgt tttttaggaa cagtatattt atatcttact
    14641 gggctatcta aactgtcgtt atccgatcca tcaggacaga gtttactatt tatttctgat
    14701 gacgtaaata attcacttat tggagaaata ttcattccac tcttaggagt tttagatttt
    14761 tctaatttaa tgatttcaca agagtcgtaa cttacatatt ttaattgttc ttctaatgga
    14821 acacctaaag gcacttctcc tacagctaaa ctcaattgtg tctttgttat aagcttcctt
    14881 ttggaattta atttgttgct gttgttaagt tctgtattta ttttttcttt aaaatagttg
    14941 tttatatttt gtgggctata tccagccaag gttaataaat taatataata catagaaatt
    15001 tccccgccgc accccataaa actactatat gagataagat actgttctga taaattaatt
    15061 cttttagaac cagtataatt taattttcta ttaatgtatc cctccattgc acccaatatt
    15121 gaaaacgctt tacaagaacc gcaactaatc tgttcttttc ctggagtaat gtattctttc
    15181 caatttattt catcattata ttcgtaaacc atcggagaaa taactgcatc cgttaataaa
    15241 aaaaatcctg tgtttccggt tttttctttt ttttgagaat taatgtcatc agtattttga
    15301 ttttcaggaa aaaattcgtt tacattgtct atattaaata tttgtttgtc gtttgtattg
    15361 tattcaggga attcataacc taaagggata tttaatattt cgtccgaata atctttagca
    15421 acccaagatg cgttttgtgc tcttaattgt tcattaataa gatctgcacg cgttggggtt
    15481 ttatttatag aataaacaga ggcgaatata aatactccga taattattat tgtaataatt
    15541 attgcaaata ttgtgtttct ttttttgttc atatttattg tctctatata atatataaaa
    15601 atgaatgttt ataactcttt ttatcctaaa ttatatattg taggtttatt tctctcggtt
    15661 aaccattgcg cgtgagccga gcgaaacagt aaagtgccat acaatattgt agctgcgcgt
    15721 tgaccgagcg caaccggtca tgctagggtt tccgagcggt caaaggagcc agactcaaga
    15781 tctggtgctt agtgcttcgc aggttcgaat cctgccctta gcatttaact tttattaaga
    15841 tgtttatgaa atttgaaagc gtaatattaa ataacaaaga agacgttata acatttctta
    15901 aaagagaaga actgattaat caattgtatg aattagagta caccaataat ttaatacagt
    15961 ataaaaaaga aaaatatatt aaaacactaa aggaaaatct atattatggg acgcaaaact
    16021 caagagaaaa tggagtttat attaatttat tacatgaaaa taataaaata attggatttt
    16081 ctatagcaaa taaagaagat aaagaaagag catatattgg aaggattgca attagaaaag
    16141 aatatcaagc aaaggggctt ggtaagaaac tattgattaa tactgttgca aaattaagaa
    16201 ctaggggttt ttctgagata gaattaactg ctaagccagc gctgcataga cttcttaagc
    16261 aactaactgc tcaagaaaaa ataaatggta aaagaagaca atataattcc gattttaaat
    16321 attctatgag tactagaaaa ttagatccac aaaaaaatga accagtaaca atagaaatta
    16381 aaaagaaaaa aagaccaata attaaaccaa aattaaaaat ataaaatcac gtgctatttt
    16441 aatcactatt tgaatcaggt aaattgacat tattattttg gggcataata tttctattgg
    16501 tatcaattgg tgttgaatta ttcatatcta aaaattttaa aaaacttata ttttctaaat
    16561 gtagttcgcc tttttgatgt ttatcatagg ctacaaaaga gccagccagt aaaccaaaca
    16621 atagtaatcc aattaatagt gctctaaaaa aattaggttt tctgggcgct tttggttctt
    16681 taattttttt tataattaca ggttctgctt ttattggcgt ctctgtcttt tttggtattt
    16741 cttttatttt cttagtattt ttaatatcag agtcgtcttt tatttctggc tctgtaatat
    16801 tactatttgt taagaaacca tccatattaa ctccccctta ttttttctta aaaacttcgc
    16861 caagaaatat tttattgctt acgccatact tctttatttc aatatatcct ttgtccgaaa
    16921 gctttttaat gtttctggaa accgtgcctt ttggcaaaaa gcttttagca gttattgttt
    16981 tttgagtctg cccttcattt tccataatta ctttaattat cttttgctcg ttttcattaa
    17041 gtcctaaaag caaatcttct ttttcagaaa cttttatttt ggttttcttc tttggttttt
    17101 tctttaaaat taaatgtatt ccaaagacta ttgccaataa aataactaaa ataattaaat
    17161 agttgttttc tgaggttttt gatgataatt cataattaat actaatgttt tttatatcaa
    17221 aaagagaaac aaccatttgc ccattaatta cttctgctga agatatcaat ggtaattcat
    17281 ttacttttgc agattttggc aaggtgatct taagctcatc aattgtttct ttatatttgg
    17341 agttataaat taatgtccaa agtccatttg atttactagt ataataatca gttaaatata
    17401 tgatttctat attcattttt tctaaatttt taatattgtc attaagatta attgttaaag
    17461 aatttaagtt ataagaaata gaatctgttt tttgattatt aattttaaca ataagatcat
    17521 agcaagggac aatgtcaaag acaagttggc tttgattaac atctgacaaa attagattgt
    17581 cttttatttt ataagtatca aattcataat gatttatttc ggtttgttga taatctgtgg
    17641 tttgtgcgaa attataatta agtattatta aaaaaataaa aatcggcaat atttttttaa
    17701 aattcataat tattcaataa tattatattg caattagtta tttaaattta cttattgata
    17761 aaaaaataag aaaaaaggaa attattcaat aatttcctta gttgcagaga ttcttttttc
    17821 tgtaattttt tcgccaatag gtataaacca aagaagtttc ctttgttctt taacttcaaa
    17881 aacatatgtt ggaatctctg aaacagtttc tagatttatg tctccaacca cttttgaaat
    17941 atcaattttg ttttcttgaa gcatttgtgt aacaggcatt atctttttta ctttattgtc
    18001 tttagttaca tatatttcat cactgttttt tacagttagt tgtttagaat ttagtgattt
    18061 tgtttcaagt gttgaatttg tatttataga ttctatattt tcatagttta tatctgtaac
    18121 ttctgagttg aggttcttat ttttaacttg tatttgtttt tgggccctgg cattttcggc
    18181 tattagattt gagttgtctg aatcaacaat tagatatctt attatgccgg agtttatacc
    18241 cgtaagtttt tctccagttt ttgagtctat tgtaacttca aatttaaggg gagtatctat
    18301 tccacaagaa tttacaaagc catccatgtt atctaatgca aattctttag aatcgtttcc
    18361 tttaacataa ctaatttttc ctacaaacag attttctttt gttgagcttt taaataattt
    18421 tctagtaaaa caagcgtttt gattatattc gttatagaaa tttattgtgg cagtttcaat
    18481 ttctgaaaaa ttattttttt cggttaatat tgttaaatca aggtaatatc cttcattttt
    18541 ctttaaatga tgggtttttg tttctgcatt aaaataagaa ctagaattac aaatagtctt
    18601 gtattgtcca gtatagtttg ggtctatttt tgcaggcaat attatctttg ttttatcaat
    18661 tgcacaaggt atctctagtt tatatgtata tttagaatct gtttgaggga gtggtttatc
    18721 agtaatttta actgcaatat ctgcataatt actaaaactt atataatcag aattgtctgt
    18781 agttgaaaga gtttctttat ctaatgttat tcttgagcta taaacagtat taacttttag
    18841 atttgtattt aaataatttt caaagatatt gtctccgctt tcataggttt caattgcatt
    18901 taaattatta ccacttatac taatagttac ttcgtgtttt aatccaggag gtgttgtgtt
    18961 tgctttaatc tctttaatag ttaaggtata tccgtcaata tcaaaggcat ctccaacctt
    19021 atagactgtc gtttgtatat ttctggaatt tatttttgcc cattctccta tagttaaagt
    19081 atagtttttt ggtttttcat tataagtgct tgaaaccctt tctatttctt taaaaacctc
    19141 tgaatcaatt atataagaat aagtatattt atatttctct tgtccagatt ctgattctgt
    19201 aatagataat ttaagatctt taattacttc attatgattg taatacgcgc ctaaaggcat
    19261 tagtgtgttt tgagaattta ttgcatttac aactagagat gaaccgatgt ctgcgatatt
    19321 agaaatatct ttagaagcag taaaatatat atttatccta tattcattat ttggattaag
    19381 atatattgga ttttcggaat tatcatatat tgggctctgt gctagtacct ttcctgtgct
    19441 taaatcaatt aattctttat atgcgccggt tattgtaact ggagaattta aattattaaa
    19501 ttttatttct cgagattgtc cttcatatat agaataatat tctgcaaaca acctagggga
    19561 tgttgcgata aggaatatag ttattgcaaa tattgcaact ataaagttta ttgttttttt
    19621 tattttcatg tttttgcctc caatttattt tacaaatctt attttaaata atgtaaatat
    19681 caatgtacca agaatagcaa ggtaaacaaa aactgcaaat atatattgtc ttaatttctt
    19741 tgctggacaa cttaatttct tcatttttat ttcacctcgc tttttttgga ataggggatt
    19801 aaaatgtggc taagtaaata gcacattata agtaactctt gttttttagt atttaaatac
    19861 ttttagtgaa actaggcgaa acttatagaa aattactgaa atttttcagg atttaaaggt
    19921 aatttgtgaa aagtttatat atttttagat gtaatatatt atatgtataa aaaagtgaag
    19981 aatttatgaa atattcaaaa ataatattaa tttgtataat tttcttaatc ttgccattta
    20041 tatttgcaga agaagaaagt gcctctgtag ttgtgccttt ggcaataaat ggaatagaag
    20101 gagaattaga tatttcaaca acccccttaa tttttccaga aacagaacaa gatgaatctt
    20161 cagacaccat aaatattttt acatctattg acaacaattt agggttttct cttaatacac
    20221 aactaaatta taaaacactt gatatccctt attatttttt aaaagaaaat caaaatctta
    20281 aaaaaaggat atatcctttt aatcaaggca gaacatatgt aataaataat tcctctcagg
    20341 aaattacaaa agacaaaact ctagaactaa aattaaattt atcagatact actcaaaccg
    20401 tatatttagt tattgataat ttgcagtcta gccagatacc agaaatgaac cgatttgtaa
    20461 atgtcacatc ttcaacaatt gaaataaatt ctgttccaag atatcttaaa ataaacaaaa
    20521 gtgccattgc aaataataaa ttacttttaa atttaaaagc aaatattact aatttttctt
    20581 ttcagatcat agaaacagat aataacaaga acgtaaacgg aatgtatagt acaaatattt
    20641 tacaactaga ttacgattct tttaacaaat acgctttgtt tcaaattaat cgtaacatta
    20701 tagaatttta ttgcccgtat tgtcatctta aagactacgg aattaatgaa aatggctttt
    20761 ctgcatgtgg gaaatctgtc tttgaagaag aaaaaaagcc aataacagaa tatttgagcg
    20821 ccaaagaaaa tgtttgtctt ttgccagatg gttctaaaat ttctggcttt actaatgatt
    20881 ctttggaagt atttggttta gacagattgt attttgaatg gaatgacaaa atcccctctg
    20941 atctttttga ttatggagaa tattatgttg atcaagacca gtttagaatt gcggtttcaa
    21001 aaaagataga cgcaattact aataataact atattgaaat aggaaatata aaatataaga
    21061 aaggcaaaaa caacttaatt tcaaaagcag gagtctatat tgaacaagac ttatctaaaa
    21121 taactgattt atccactaat aattcaataa tagaatcttt ggaattttct atcgcggtat
    21181 taaactctat tccggaagaa tatagaaaat taaccatttt agacttaaca aataatgagc
    21241 cagacgttag tgattcagaa tttaatagat tgctgcaaga gattttaggt ggtaattatt
    21301 ataaatttac agattatcac acccaccatc aaataacact agaaaaatat ctctcttcta
    21361 ttaataattt taaagtttct agagtaaata acgatattta ttctaaacta ttatatggct
    21421 tagaatacaa aacacaaggt tattctaata tttattattc tgaagggctg aattatttta
    21481 tatataatat taaaccattt acagaagaaa caaccggaat actaaataac gaacacacac
    21541 aaatgtttgg taattattgg aaactaaatt taatagattt aaataataca atagaaccaa
    21601 aaacagtagt agtaaatgtt tcagaaataa cccctgaaag taaaagagca aaaataattt
    21661 tcaataatcc ttcaacaaat ttatatgtaa attattattt tgaaaatcaa tatttttcta
    21721 gtaatccatt atttttaaaa acaataaatg cattaagtta taatcgaggc aatatttttg
    21781 aaaatacctc ttcttttacg tctaagcttc cagaagttat tcagaactat aataatctac
    21841 aagatggtta tattgtttct attgattctg tacattctga ttttataatt aatttaacaa
    21901 aaccagtaat aatcactaaa ggtgcaggag aagaaattct ggttaaatat tataatggca
    21961 gagaatatgt taattataca aacaacctca caactataaa taactcaaga gaggatttcg
    22021 caacaaaaga aacgcttgta atataccctc caaaatcaga aaatacaaat tatccaataa
    22081 taatagaatc acaaagccct ttaatcttta atagtgaggt ttttgaaagc acagaaaatg
    22141 gaaaacacat atatagatta aataaggaaa atcaaaattt aagatttctt gaactaataa
    22201 atgacgtata caataacgaa gcgtgctttt caatttctga aaaccaattt tcgttatgga
    22261 aaaatataga cgaggaattg gatattaaaa aggcagagag ctatttggaa tttgcaagtg
    22321 ctttacaata taatgttggc gagtctttag agaatcaaat atctcaaaat caaaattcac
    22381 aaccaaacaa ttcttttgca gtatacacaa ttgatgagtc tcaaacacta tataaaaatg
    22441 cacaagcagt taatactttt attaagacag aaacagataa tagaaactta aacatattaa
    22501 aagactatag cgaggtttat gaaaaagcaa tagaaagcga accaacatta tataaagaat
    22561 ttagagatct attaaattgc aatgaaacat taatctgtag aatttctaaa acaagataca
    22621 ccccgcaagg atattttttt gagagattta aaaacgtatg tgttgataat actccaacag
    22681 attgtgttag tttttggtca aatccaaaag tgaattattg ttctgcattt gttagaaatt
    22741 ttaataataa tttctttgga tatagatttg attcagcaga tgcttgggat ttagctaaac
    22801 aaccaaataa taaaagtatc tggaaagcaa caaccgattc attacaagaa aaagattttg
    22861 atttattaat tcccgggtca gttcttggaa taaagcataa taatacaagc tataaaaata
    22921 aagaatatag ccatgtagtt gtttatcttg gaaaaattgg ttcaaggcac tatattattc
    22981 attcttgggt aaatacgtta aaaatagaaa gattagatgt tttcttaaaa actacagcaa
    23041 gagggaaaaa tgcatatggc tattatgaaa atggtcaaat aaaagaagta atgatttcaa
    23101 ataatcttaa tcaaaaacta attgcaaaag ccagagagaa cggcatctct ttagggtctg
    23161 tggattcttc tatttatcaa ggcattatcc cagattatat ctttgattca tttaataata
    23221 ataaaatgtt gttaacaaat attgaaaatc caagtaataa tgccgtatat caagagcttg
    23281 aagaagttta taatagaata ttacaaaaag actttgatat ttattataag accaattact
    23341 atacagaaga atctccagaa attaacgaga aaataagatt ggattctaat gagccaatga
    23401 ttgtaatact tggcaaatac aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnaaaata agattggatt ctaatgagcc aatgattgta atacttggca aatacaaaag
    23581 attattttta gtaaaaaaag aaaacaatag aacaaatata atttctgaat atcctttatc
    23641 tacaggagta aatggctttg gttgtgaaac cgatagttta aaaacgccaa tgggcttatt
    23701 taaaattgta caaaaagttg gacaaaattg ctcgcctttc caaataattg gagctagtgg
    23761 ttgccaatat agtgatggcc atcctgttat tgcaagtaaa aacacaggaa ctgcatacgt
    23821 cgttacaaga aagctagtaa ttgatggtct tgaaaaagaa aacataggat taggttgtga
    23881 taatggtaac agaaatacaa tctatagagg gatatatatt cacggaacaa ataaagagaa
    23941 ctcaatgggt cagcaaagat ctcatggttg tataagaatg ctaaataacg atgtgatttc
    24001 tctttttgac cagatagaaa atggtacata tgtttatgtt tataactccg agatcagtta
    24061 ttctaatctt ttagatttag aaaatcaatt tgcaagtaat ggaacaagct taaatcaatt
    24121 tgtatttttt agttggttta atctattttt cccaccagtt atagagccca caaggccaaa
    24181 acctaaaaca aaagaagaaa taattaattc tttagccaaa ggaacaggta attgttatac
    24241 cactaatggg aacacattaa ctacattaag atcaggagat ttaggtttaa caacaaaagt
    24301 aaataccaga agaacaaaaa atagtgtggt tgttccaagt acccactctg tttcgttatc
    24361 agatatttat acaattgcaa aagataggcc aaataattat agagcagaca ataaattttt
    24421 aactgcaatt ccgtcactat ataatctttg tgcgatatat ggcgtagatc caatttttat
    24481 tttagcattc tttaaaagag aaaaatcaat gtataatatg ttagcaggaa attataataa
    24541 tggagagcta actgcgacaa acaatccatc agggataaaa gcaacaaatg gctgtgcttg
    24601 tggtggtagc aaagcgcgca atactgaatt ttgtgtatac cctacgttag atgctggttt
    24661 tgaagcaacc ttaataaatc tcaaaaataa ttattctggt agagtattat ctactggttc
    24721 taacaagaac agaggcgttg cagaaaaatg gaatcctggt ggcacaccaa gttatggtag
    24781 agatttagtt aattttataa atcaatttta tggttatatt aactaaattt aagtaaaggg
    24841 tctctaatct ttagagtttt tcctttcttt gcttttgtag aatagtacct ttttccgtgt
    24901 tccaccaaaa gagcatgata ttcttgatac actttaaaat ctcttggaag gttatccata
    24961 aatagttttt gtatttcttc gtattttgca ttctttttaa ttagttttag atttagtaat
    25021 attcttctag tgtaagtatc aacaacaaat attgggtctc tatgagcata aagaagaata
    25081 gagtctgcag tttcttttcc aataccttta acagaaagaa gctcatgtct tgttggcatt
    25141 cgatctttta aagttagata aaatttagaa atatcaacaa gtctttcata tttttgatta
    25201 taatagctaa gattatttat tgcgtgctta attgttctag aatcgctttc aattattgct
    25261 tttggattta acagatcatt ttttttaata ttaaatattg ctttttctac atttgtccaa
    25321 gagtttgctt gtgttagtat tgctccaaca tatacttcaa atatctgttt atgtgtttta
    25381 attagaaaat actttcctgg atgatagcca atatctgaaa gcggccacca gccttgtggc
    25441 ccgtagttaa taaataaagt attatatatt tcttttattt cgttcatatt tatctgtagt
    25501 ttaatttgat tattataatc tatatataat aaaatatatt tataaatact tcagctcagt
    25561 atatttaagt tgaattttgg ttttttgggg atttaaaatg aatagaaaat ttcttgtttt
    25621 tataactata tttgctattg ttctttccct taatttaatt tacgcaagca ctctttctgt
    25681 tgataagaac gaaacctatc taggagacac tgttgtaata acaggaaccg gattttctcc
    25741 aagttcaaca cttgttttac aaaataacct tgagccaaac tctgcttggt gggatggata
    25801 taatgaacaa tggggttttc caacctttgt tacaacagac gaaagcggta attttacata
    25861 taattggaca gtgccttttg attttagaat ttctccaggt aaacaatatt tttccgtagg
    25921 caataatata tctgcaacaa tttattggaa tggtggagga gattatccga cttctaaaaa
    25981 taaaacaata tgtgatggga attattgtta tgttacttca acatatacaa atccaaatgg
    26041 cggtgttgaa aaatttaata tgtcaacagg agcaagagaa tgtaatgctt ctgttgcggt
    26101 tacaggaaca ggaatagcaa tagatgggac atatatatac acaacatatg ctgcaacatc
    26161 aaacaatctt agaagaacag caatggcaga ttgtacagga acatcaacat ttactactgc
    26221 cggctctaat acatattctg ttgctatttc caatggttat gctttttttg gttataatgt
    26281 taatattgcc ccaagaaaat atgaaaatct ttctacaact ccttcacaag ttgtagcatt
    26341 tgcgccagca tcaagaaatt ctattgcaaa ctacatttgt gttactcctg atgcaacaaa
    26401 agtcgttgtt ggctatgctg atggtaatgt gttggcattt gattacacaa caggagcaac
    26461 attatggaat tctgatccag atgatgatgt tactcaagca atttctggga tgcattgtga
    26521 taatgacaaa atatatgttg caaaagctta caactctaca acaaacgggc cactgtttgg
    26581 tggttttgca tatgccttaa gtgcttctga tggagctttg gtttggagaa gcgaaagaat
    26641 agatagtcat tatgtttata gttcatatac taacgttgct tgtgattctg taaattgtta
    26701 tgcgtcagtt ttatctcctc cactcggggg tgcaggaatt aatggtgctc caattattca
    26761 aatagacaag actactggcg aaattggttg ggtaaaatgg agatcttggc ttccagcaag
    26821 cacgggggat tatggtacta gcacaacaca tcaaagtcca atggatttgt gggtggattc
    26881 tgatgaaaat ggttttattt atccggttta ttctggaggg agaatacaaa aactttcaaa
    26941 aaatgatgtt agtgttcaat ctttcccaga tacaaatctt tggataagaa gcaatactaa
    27001 tctttataat tttgtaatga acccaacaaa gaatgttttc tatagcaatg aaagaccaag
    27061 ttttaatttt aaagcaatag ataactatgg tcagtatatg gacgctgatg ataatagatt
    27121 taatacctct catttaaaat ttagtacaat taatgcttat aatgaattta ctgcagcaga
    27181 ggccggttct gctacagttg cctcacaatt tatggatcca agaattatgt ctgtttattc
    27241 tgcaacagca acacttccaa atcctacacc atataattat ttaaaattca tgtatgacga
    27301 atatgacagt ggtgtatttt gtgaaactgc agtttataac acaaaattat ttggttggca
    27361 tatcaacaac agatattgtt tatatgaaaa tctagaagtt aataatgcaa cacttaatca
    27421 cacacaaaat ggtgtaaagc ttgcttctat tgcaaattac tttttagtaa atcctaataa
    27481 atggaacaat tttccaatca caaaaacatt tggaacagat aattggactt ggtcatcaag
    27541 tcacaaccct acttatgatc aaatattagg ttatgcaaca agatttggtt ctgattttaa
    27601 tgaagcttgg tttaatcaat taggtcaagg aacttctttt aacacaaaca caggaacaac
    27661 ttctttaata gattatattg atgcaactca agattttaat aatgctgatc caagattaca
    27721 aaaatggcat gaatcttatt tcttatttaa acctcttggt tcaggggcag acggaaacag
    27781 acattggcaa tatattgaaa atgacactaa tatgattgct actccaataa ccgttgttca
    27841 aggaacaaat caatctggaa gttatttgat tggtgcagat actttttcaa atagaataga
    27901 tttcaatgcg acagaagcag gaatactaga aagaacaaac gaaccattct ggtttgatgt
    27961 taatttcata gatagcataa attcagattg ttcagacatt gttgtaacag acattacaga
    28021 tactccaatt ggtttatggc aacttgattc tgaaacttgt acagcaaata attattacac
    28081 attgtttgtt caagacaatt ttactttagg acaagaaaaa caatatagat tatattataa
    28141 ttctccaaca gcagcagtta ctccaggagg aacaacagac ttaattacta gtttagtttc
    28201 tggaacctgt gctgcaggaa cagatattat tgatgtaaat agtactaatt ggtatagatg
    28261 ggtgcagcca ggagttggtg cagtagctgc taaccaatta tattttaatt ttggtggaag
    28321 ttcaaatact tggtttaatc atgcgacaac aagatatgcg ttaagagcag ggtatttaaa
    28381 tagtcaatct gcaggaacgg gtgcagggtc agtaacaaat acacttggaa ccgctctagc
    28441 tacttttact tcaaggtgtt atcaaagaag ctataataaa gacaagatat ttacacgaat
    28501 tgacatgaat actgcaaact ttacgattgc tccacacact ccaaccgaaa ttaatccaaa
    28561 tgcaacaaca acatttacaa ggaagataat gagtcgttgg tatttcttta atggaaatgg
    28621 aagaataaga ataatgttta aactggatga accattacca gtaatgacta ctggtatttc
    28681 acaaacatat cacccttatt tttattttgg aatgtatgct ttagttccac aaggaacagc
    28741 aacagttgct gcaaataatt ttgttaactg gtcaggaaga aaagaattaa tggatgaata
    28801 tactcctgta caattgtctc ctgataaaaa gtcagcgata ttaactccta gtcctttacc
    28861 attgactttt acaatagact ataatggtga acacttccca acggtttctt ctgcaagtag
    28921 caaggttggc ggtggttgta ccgtacataa ggtttggcca tatttttctt ggagaccaat
    28981 tgatcccgta acaacaacaa atgcaacaac ttttgcttca gcttatacta ctgactttat
    29041 atcttgtgat gaagatttaa atggcacaat tcctgatatt ggaaatgatg gttatgttaa
    29101 tgtatttcca aaagaatcaa tctatggaac aggattaaac agatttgttg acccaaacta
    29161 tattgcgaag ttttatttaa agccaaatct ctatattcct gacaatagag aaaacagatg
    29221 gcttccacca ctagcattaa attcacaata tggaacatct gcaaaagtaa gtacaattat
    29281 tttagatgtt tgtccaacag aagaatatgg ttataaaggt tatgatgatt ttttagcaga
    29341 tttagatgat ttaatctcaa gttcatcaat acacaaagtt gaagaaaaca caactaatat
    29401 tccttgtgca acatctatta ccatttttag tactaatacc ggttcgatag gtaaatattt
    29461 cttattccaa agagatgtta atgttgttgt agcagatgtt tcacgaccag atgcaccata
    29521 tccaagttat ctttatgatc caacaacagg tgctttatca ataaatggaa gtttagatta
    29581 tacaaatgct caaaaatcaa cacaatatct tggtaaatgg ttttttacgt caagttctga
    29641 tccttatgta ataagcggtt atgcaacaac aggtgctgaa caattacagg gtgctaaaag
    29701 atatgcttgg gcaccaagct ttgcttttaa taagaaagtt tattctgaga atttattttg
    29761 gaataaaagc atgtcaaatt taacttatca acaaagtcct tgtgcttgga atttttcaac
    29821 aaactattgg ggagacatgg attttggtcc aggctggaag aaaacaatgt caacgactca
    29881 gcaaggaaca atttgtccaa gaaatgaaag taatatggaa cactattctt cttttagaat
    29941 ggcgcttgct tctttgtatc cagcaaatgc tttagttgtt actgcgattg ataatgtaat
    30001 agatggtaga ccaaataaag ttacaaccat aaatgatctt ggtattgatg catcaatgtt
    30061 ttctagacat aactctttat gggattataa tcattatagc gattacacac aagttttaag
    30121 tgatttttct gaattaacag aaggcacaaa acattctaat gatgagcctt ggcaatgtga
    30181 tttctcacga tatgattcta aaaatttctg tatagttcct gcaacaacaa caacattaat
    30241 ttatactgca actcccggag tattaactgc ttctacaaca agcggcatgt ggtctccata
    30301 ttaccaatca aatagagaat attctgttct tagaaattat tttaacaatg tttggtttaa
    30361 tactgccgca atgtttccaa catatgattt tttaacagaa aatacaatct ttccaatgct
    30421 tggagaaaca gaatttactt atgatacact ttatacaagt aataatccaa acgattataa
    30481 tgatttaatg gataaattat ttccaagatt gcacggagca ctttatcaaa atcaattcct
    30541 tttaagagat tggaaaaata cagcatttat ggttctttca ggaggagaca caagagaata
    30601 tcttcgtgga acaacaatta atgtttcagg atttatgatg aaagatagtg acagtctagc
    30661 aaatgcgcca atcattgttc aggtctttaa aaactctgca ccagaatccc cactaacaac
    30721 ttatcaaaca ataactgatg ccaatggaga ctttaattat tcttatacaa ttactccaac
    30781 agcagaacca ggtctttatt tctttaaggc aatttacaat aatggagaaa taactgggac
    30841 aacgtcattt aaagtttcgg ctcttaatat tgcaacggtt ttagataaat cagtttatgt
    30901 tgcaggagat agtgtttttg ttacaacaac aataagtgat gcaataattg gagatttaat
    30961 aagtgatgca acagtcaatt tagtaataag tgggccagca ggaaatactt tattaacgac
    31021 ccccatgact aatgccggat ttggagtata tacttattct ttccagttgt ctcctgaaac
    31081 tgtaaatgga acatatgtag taacaattac cgcaacaacc gcagataaca caggcagtgc
    31141 aacaacaaca tttggagttg gtgtggcagt aagttcaatt aatataatta acaatttcca
    31201 gatttctgat gcaaatatag aatatgtaac tcaagtaaca actttacaaa atgttgttgc
    31261 aacaccacta ttagaattta ctatcaatta tcaattgtct ggtggattag atttagaaaa
    31321 cattacttta tttacaaaag atgatattgt tcttgaaaga gtttatgatt ttgatgatct
    31381 taatagaaca acatattatc tttcattaga ttcttcaaat aatcctatag tttatataat
    31441 ggcaaacttt gaagcaagtc aaataagaac aatgactttt attgcggaga ataaatatta
    31501 tgaacacata gaacaagatt ataatagtgt aaacaattat tcttctgaac tttcaagtta
    31561 ttgttcaatt cttttaaccc ctgctgccca agcagattgt gcaaatgcaa atacttattt
    31621 aatagatata aatgattatt acactcaagc acaaacagca tcaagcatag atcaatttta
    31681 caatataaaa atagcaattg gtgaaaaata tattttgttt atggacattt tagattcttt
    31741 aagaatctta gcaaccaatc aagcaagctg tcaaatattt gttccaggaa cttggtctct
    31801 tgattctaat gtttcttttg ctgtgttaat gaatgggcca tcaagcattc caattaatgg
    31861 tttgactttc gatgtcaatc ttttcaatta taattcagtt cttgttgatt catattctgt
    31921 agatgaatat ggcgagggat ggtattattc ttctttttca actccaagta cgggcggagg
    31981 gtataatcta agaattgatc aaaatattgg gacaaatagg ttccagtgtg ctgcatcttt
    32041 tgatgttaat caggcatctg gatcagtaat tggtggagag attggggggg gattaactgc
    32101 agatcaaaat gcaacgcttt atgagatatt aggaattgtt acagacatta atgcaacatt
    32161 aaatgatctt gaagaaataa tatttgatat taatgcaata gtaacagaca taaattcaac
    32221 agtttctgat atagattcaa gattaagtac tcttgagaca agcctatctg atattaattt
    32281 gttattgact ggaattgatt caaatattaa ttacataatt ggcaacatgg ctacaacaac
    32341 agacataaca aatttagatt ctactttaca aacaatttta ggttataatg tagatattaa
    32401 tcagacagtt catgattctg ctactcaact aaataatttg tacgatttgt tggacgacat
    32461 tgatgatcaa atagatgcaa tgaataatac cataactgat ataaacgcct taataacaac
    32521 ttgtgaaaat gctccagaaa caagtatttg tgcagatatt aatggtttat caaatcaatt
    32581 aaataatatt aattctacaa tagatattat ttcttcaaca acacaagata ttaattcaac
    32641 aacaagctct atgcaaggca caataaatac tttaaacaac acctcaaaca gcattctttc
    32701 aacagtaaca gacattaaca atacaagcac atttattctt tctaaagtaa acaccataga
    32761 atcaaataca caagatatac aatccaatat aacaacaata atgtctgatt taaattgtgc
    32821 aggaactccc ccagaatata gtgtttgtgg taatcttcta aatatcagtt ctttaatttc
    32881 tgattttaat attagtacac aattatcact attagatatt tctaatgcat tatcaacaat
    32941 agaatataat caacaagata taaatcaaag tatatcttat attttagaag atttaaattg
    33001 tgaagacgaa aatgctagtc caatctgtag cagtatacag accataattg ataacactga
    33061 taccttagaa acaagcattt cggatttaaa taatacaatg ataataattg gagacaatgt
    33121 tttagatatt aatagtaata ccaacaatgt acttttagga atatctgatt taaatagcag
    33181 taatcaagaa attctagatg atctcttaaa tatttcagaa gaaataacaa atactcaggg
    33241 attgatttct gataccaatt taaatcttac aaactatcta tatgatatta atgatactgt
    33301 agatgatata aatgacacag tagacgacat aggtggaaca acagggggaa caagcgacaa
    33361 ggttgatgag ataaaagaca tagtactaaa tatagataat aatgtttctg aaattaacac
    33421 cacactaaca gacattaata cacaacaaca agcaattcaa gcaacgctag caagcatgca
    33481 attgacttta agtgatatta atgacactac aacaaacatt tacacagacc aacaatctag
    33541 atttacggtt gctataagtg attttagtga aattggttct ggtgacgtct atagagcaaa
    33601 agtctgggtg tttgattatg atggttctcc aaaagatgca gatagcaatg atgctaaaat
    33661 tacactttat gatccagaaa gaaacataat tacagaaaat ataacaatga caagattatc
    33721 agaaggagta tacacttatg attataatgt aggaactggc cagattgcag gaatctggga
    33781 agcagtggca accgttttag taaatggaga aaatgttagc ccaagtgatt tctgggaatt
    33841 agttggtaat cctccagaag ttagaattaa ttctattgtt gatacaagta ttccaacaat
    33901 aacagcagac gttataatta caaacgaagg aagtgcaagt caagagtatc aatacgaata
    33961 ttgtattgtt tctgatcaat caaatatgtg tggtggagga gacgatgttg attattcaag
    34021 tggtgcaaag ttaatacttc ccggacaaac gtggaatacg gagttgactt taaatgttcc
    34081 aactgttgga acatattggt ttaagattaa agtttattat ggtgtagaaa tgagtgctgc
    34141 ttctaagcag tttgtagcaa cgactggtgg aacaaacact ggaggaacag gcggaggctc
    34201 ttctgatata agcgacgtta caggccttgt aagtttgcct gttgctgtag gagaaattgc
    34261 aattatagaa tacccaacag atatgacaat aattcaagga gattatggat tttataccct
    34321 tagaataaaa aatattggaa gtattgattt atcagacatt aaattaacca ttgaagattt
    34381 accagaaagc tggtgggaga tacaacaaga aaagacaata ttaagtccta atgaagaagg
    34441 cagctttgtt ataaagctaa aagcaccaat ggatgctgaa gtaaaagata ctcttgttaa
    34501 atttaaaata tcttcaaaag aagacactcg tttaagagaa agcattctta gagttcttga
    34561 aaaatctcta gcagaaatta gatttgtaga tgttaaagtt tctccaatta aagtaaatgg
    34621 ttatggagag attgagttta ttattcagaa tatgagccta tatgatcaaa atataactgc
    34681 gacacttctt gttccaagag attggcaaat agaagatagc gatcaagcta ttattttaaa
    34741 aggcggagaa acaaagcgca taatttttgt tgttaatgca tcatatatta gcggggtaag
    34801 agacttagtg tttgtgataa agaacaatac cggccagaga tttaaaacag gagaagataa
    34861 actatttaag caattgatgg taatagtaat aaatgacgag aaagtaaaaa gcgaattgaa
    34921 tttctttaat cagtttaatg gaataggtat tggtattctt ataattatct tgttattggt
    34981 tattttaata ataatctttt tatttggcat aaaaaataat aaaaaacaaa gaccatcttt
    35041 aggatataat agaaacattt tagataaatt aatgggtttg agaagaaaat gatatctgga
    35101 agtttaatgt ttgtgttgtt tataaatttt gttataatca tgtgcgtggt tatttttttc
    35161 ttaaggcaca tgtctttaag atttagagaa gaatttgacg aaacaaataa aagactagaa
    35221 atgattgaac ataatatgca acacaatgaa ttgagattaa aacatttaga attaagaata
    35281 gacaagcacc atgatgtttc tgagcatcac tataaaaaac cagagataca caaagatatc
    35341 gaagacatga aaatagttgt aaaaaagata aatcaaaata cagaaaagat ctataaaaaa
    35401 acagaagaac tattcaaaaa gtaattttat atgctaagaa aactaattat atttttgtgt
    35461 ttggttcttt tcttaggaac cattattttt ttattattaa actttaacac aacttatta
//
LOCUS       DAOOON010000009        34349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_1460_length_34349_cov_15.610602, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000009 DAOOON010000000
VERSION     DAOOON010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 34349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34349
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1460_length_34349_cov_15.610602"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1118)
                     /locus_tag="PLK55_02180"
     CDS             complement(81..1118)
                     /locus_tag="PLK55_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOZ35766.1"
                     /translation="MKNKEIIKDSSSNEFLFYTTPTGDVKIEIFLHNENVWLTQDKIA
                     LLFGVQRPAITKHLKNIFENQELVEESVSSILEHTANDGKKYLTKFYNLDAIISVGYR
                     VNSKKATQFRIWATKILKQFAIKGYVLDRKRLENGSFFNKNYFNELLDEIREIRLSER
                     NFYQKITDIYSTSVDYDKSAKTTRDFFSKVQNKLHFAIHQNTAPELIIKRADAKKENM
                     GLTSWKNAPTGKIIKTDVSVAKNYLKKEELESLSRIVSAYLDLAEDRAKRKIPMSMED
                     WSKRLDAFLKFDERELLKNAGSITQEIAKQHAESEFEKYRVVQDRLFKSDFDKLLEKT
                     KDITKNTQKKK"
     gene            1259..2623
                     /locus_tag="PLK55_02185"
     CDS             1259..2623
                     /locus_tag="PLK55_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOZ35767.1"
                     /translation="MNIILIIVIVYFVLMLGVAWYFSRKENIETYFLNNKKTGLWLMV
                     FSNVATLIGAGAVVAMVSEGYNSGISYGLASIVLSIVGMIILAIFAKRLRQYSENNNI
                     YSIADFYEKRFGPNNKKLLTILQPILMIIWVAVQVIAISAMASFLIGINSTLAIILAV
                     GITIIYTTLGGLKIDIISDCIQFWMILIILIAIAIFGYFQIGGLNQLVLSVPKGHLNP
                     FAFGGLSWFIGMVLFSGFMYLGNGAHWQRILSAKNSDVAKKSYYYSIPFMIIISVIII
                     FLGLLAAVILSNINQDQAIYLLMYKTLPVWMVGLGFAAILAVVTSSIDSLMIGGSTII
                     YKQFFSTKEKNNLLYARLITVLFGIISGVIAFIIPSIIKLSLFVSYMALMFVPSVIAG
                     FVSKKTSGKASFYSILLSVICLSGLYFFTSYSKSSFILGLLIAVITILFYDKVFKKDA
                     NKAF"
     gene            2647..3471
                     /locus_tag="PLK55_02190"
     CDS             2647..3471
                     /locus_tag="PLK55_02190"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOZ35768.1"
                     /translation="MTIQLKQFTPGKGAIYYFSGTGNTAFVAFEFQENYKKANVSVDL
                     FKIENTDIVSSQGKYDFLMLGFPVYAFYPPDLVVDFVKKLPEVKDKPVILFATAGITT
                     GISFNVLENILKNKNYNVISNFAYIMPDNAYFVFRKDTDKDEQIELSLNNTIGKINQD
                     FDILISGKSKFKKANVFWSLFAPLTKFFFFKILQPKKNRFVWDREACNYCGLCAKICP
                     TKNITIKKEKGKVIFGNNCMFCTRCYHFCPQKAIHNKSINRTKKYRRYTRLKNEII"
     gene            3482..3940
                     /locus_tag="PLK55_02195"
     CDS             3482..3940
                     /locus_tag="PLK55_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOZ35769.1"
                     /translation="MEIINYSYEEIEKLCKGISKKIENSKFKVDYILGVSVGGLVPAV
                     IFARLLKTKKIMSVSISSYEKKKQKELKLLIAPDKSLLKGKNVLIVDDICDSGNTLIF
                     LENLLKTYKVKCIRCATIFLNKEHHKITPEYYGKVVTKWIDFPWDKFEKE"
     gene            3944..6475
                     /locus_tag="PLK55_02200"
     CDS             3944..6475
                     /locus_tag="PLK55_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOZ35770.1"
                     /translation="MLKRNYVLLFLIFSILIFPTISAKSFAITNYDINYTLEQSGIVS
                     VSEKLDYKLSGCYRELFLQRPNLEILNPSGYCQGAACSFEYKQTNTESGDPELILKGN
                     FCDITVTANFNYNLVHQIRALEDGTQFYYQVYPGKTSVSTNTKISISFPGDLNQTTRF
                     IHSRNYQSSATESKLTISKSVAAYEIIEVNILMPKAWFIEDNLYRYKDVNYTQVQIIQ
                     QEQNWQEEYDKYNQNQSITSSRMKYAINNYDINYILNTSGIIEVSEKIDYTVNDCLTQ
                     LSIQRPNDITNPSGYCVGATCNFSYKTKNTLSGYPELILDGNFCNINVTAYFNYNLEK
                     EISGLLDGTQLRFDIYRNKMGVPTNINISMFFPGDANKVTTFFYPSDYLAKTDGNVLL
                     YERAVNEFDTVYLLAVMPKEWFDQNNIYYQSNYTVSQIIEAEEQKLKLQEEQRLKIEE
                     ARLKQIEEQRLILEEQRLNQYSKVNQNFMPGIKSQEPNTDWLLNFLLYSGILVVILLI
                     IWFIFGRDYSPEQVNYLGIYERDLPGDDDPLKAHYLITGEFSKDWFSSAILYLVWKKQ
                     YNLEKRKDNYFLIRNKSIKLGNLPSYIMKIDKFLNKHYPEGEIDLDLLKKRLKSQSLF
                     SEDYRQNYKKIIAQGYDFTVLYQKIYTEYDSWFNKKSKLFDQKGYIIATSIFSIFIVF
                     SFFFNSAIFVMSIIVTILGSVILLLIPRLFKFRLIFGKFSKEGRIKNLQWTNFKKYIT
                     DFSLMRAHPPQHVILWEEYMVYATAFGVAKKTSKVLEIVMPQEIRSNQMVATYSGFSS
                     ISSDFSGVGGFSGSGGSAGGGGGFGGGGGGGFGGGGGGGGGAGAR"
     gene            6549..8963
                     /gene="ppsA"
                     /locus_tag="PLK55_02205"
     CDS             6549..8963
                     /gene="ppsA"
                     /locus_tag="PLK55_02205"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOZ35771.1"
                     /translation="MVSKEKKFIMWFDEIGIEDIPYVGGKNASLGEMYQNLGKKGVKV
                     PFGFAITAYAYRYILEKSGLKSEIKKTLKDLDTHNMDSLAEKGHKVRHLIRHADIPKD
                     LEKEIFAAYDKLCKIEKINNLDVAVRSSATAEDLPDASFAGQQESYLNIRGKEDLLEA
                     VRKCFASLFTNRAISYREDKHFDHFSIALSVVVQRMVRSDLASSGIMFSIDTETGFRD
                     VVIINSTYGLGENIVQGAVNPDEFIIFKPTLKQNKKPIISKVLGEKAIRMVYDTQGNK
                     PTKNLSVEPNDRNKFSITDEQALKLAKWACDIEDYYSKKSGKFKPMDMEWALDGKTNE
                     LFIVQARPETVQSRKDLNSYTTYVINTTAKPVVVGKSVGQQISTGKVNIIKDAKEINK
                     FKKGEILVTEMTDPDWEPIMKIASGIITNKGGRTCHAAIISRELGIPCIVGTLDGTTK
                     LKAGKNITMDCSSGDAGFVYEGKVPFTKQTVDLKELQKTKTQLMMNVGNPEQAFDLSF
                     LPNEGVGLAREEFIINSWIQIHPLALINYSKIKDAETKEKIDALTVGYKDKKQFFVDK
                     LAQGIARIAAAFYPKKVILRLSDFKSNEYANLIGGKEYEPEESNPMIGWRGASRYYQG
                     NYVAGFALECQAIKKVREDFGLTNLELMVPFCRTLDEANKVLAEMKKNGLEKGKNGLK
                     VNCMCEIPSNVILADKFLEIFDGYSIGSNDLTQLILGLDRDSELLGNIYDERNEAVKK
                     MLTDVIKIANKKGKYIGICGQAPSDYPEMAEFLVKEGIKSMSLNPDTIIKTRMAIAKL
                     EKSLKK"
     gene            9001..9462
                     /locus_tag="PLK55_02210"
     CDS             9001..9462
                     /locus_tag="PLK55_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HOZ35772.1"
                     /translation="MKSILFVCTGNIFRSMSAELFLKKYLLERNKKIKVSSAGIQANP
                     EGIHPIVKEELDKREIDYSKHVQRKVTKEILDSSDLVVSMTYFHQDFLKEKFNQDSLL
                     FNELCFGKKEDFLDYPDILIETNSKDRAEEYIRTAIKEIEKGCKSLAKQLQ"
     gene            9535..10092
                     /locus_tag="PLK55_02215"
     CDS             9535..10092
                     /locus_tag="PLK55_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOZ35773.1"
                     /translation="MAIALIIILVILGVILLMALFMYNSLVVMRNKVKNGWSQIDVQL
                     KKRYDLVPNLVETVKGYAKHEAGTLSKITELRNQAMQTKDIKAAMKTNDQMSKAISGL
                     ISVVVENYPDLKASQNFLMLQEELSGIENKIAYARQYYNDTIMDYNNKLQVFPNSIFA
                     KMFNFKEETFLEATAEEKKNVKVSF"
     gene            10260..>17765
                     /locus_tag="PLK55_02220"
     CDS             10260..>17765
                     /locus_tag="PLK55_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35774.1"
                     /translation="MAKEKAKPKGSKAKKEETDFEDTEIDDYSEVDEEFSEENIGKQT
                     ENNIFGKAYKWLETKYYSFSDWLTKKGINLNKANDFLEEKGIPAFVFVASLTLIILAV
                     LIFLIINFATKTTVEFSIVDFSNNPLSDVDVKIYDLKDKTKFNGTISDGHKARLSLKS
                     GINYTINATKSGFADYEKDNLFTRGEPIKIKFSENVTLGNFKLSVTDLETKKAISNYR
                     AILSYDISGKPQEQTATPGSANQISFTDVPLGKSLSLVIEADGYQTVSKSITVSNNEE
                     IIPIELQFDLDSAQLEGLDARGTIVAVSDTGDVLDDCEVTIYNISDEKIATGITQLGR
                     FTFTEKVGSVIRFVVTKEGYKTYDSDEENKTFRLLRSEDTFTATLKKGSSDLKVMVVE
                     DMVGPVSDADVSLYNLVGNLLTEKTTLLDGEVIFSGLDKNKDYIVTACKEDYLCSQYF
                     VNIDNGNDLEAKLVKLGSAETYKLSIYVYDGANAPLSTANVLVYKLLNGKYVPIGFGP
                     LQVDLTGYTSVFTQKAETYKVVGIAGDSNASQEITIDQFKDNKVILIINDASKEVTLQ
                     LKDIYGNPVIDGYVIVKSKTGEILYEGYIDAENPAKFSTKGYKDLIVEYLDEEGNTTV
                     ASAVVGSGEDLVVSLKPMIEDFNPIISFLEIRDLQNNSTSILSKDKDYYLVFDLQLPV
                     DVKKCGVHFRAGKDSENDSENMDYGITGFKADTTSFKYSTTYNEGQQSIDFDNVGSPN
                     ELNKWLELYWPDVSLTNKQIMLKVKATEIGDLLLRYRAWCEDSEGKIYRDPEDTTLGT
                     ARNNSSKQSLYAETKEQVFKVFETPAECSDNLCVEYKFLDKGGFDYSNETFFGTVDEM
                     YLLELNYYSPKSGELTIDTETDASHPIIGLINYQDTDLFPSSEQDSQETKLSSMAVSL
                     QSGIYRKQYLLFNAKNIGVTYIDIKSTFNSNVTEKRLTFEIKNKRNLEVNVSELLPYN
                     TPITIEVKDADNKSPINNALVKLEDEFGDLISSTKKAKAGKYIINQNFSSSKPNLEVM
                     APGYAPYTQQLMIADTGLISGPDSISINFGNEISQETEIITLVNKGNAKITDLTYQIT
                     PIDEVAELTIETQLPVTLNPASPSEIEVTSVIDPKLKFKTAKYVLTIFGFVGNKQVAK
                     DIEIEIYKGQVQENCLDIKPLAIYSYVGISEGSENEITATLVNNCKKAITVTPELLKA
                     KGTVIKKDENLEIVLPSVTIEPNEEITDYTITIKNNKARKTTKSYIFEIAWRNAYYAL
                     PNTKLNVDLVDYSKTIKIIPPVSIVSMSQFNEEIVASNKTVFSITNTGKYPLKDIQIS
                     RLEEKYVSNIQDKIEPITFEEIKPGQTKQVSIIYEGKVSNATRATLFYKVTAISPGSK
                     DPIDAKFTVDFRISSASCLKIDQKKIAFYTRIGEEKIKLINITNQCAEPIAYIDYDIN
                     PNRAPNDFTLAFGEGTGVALVPATPIPFIGIGQTVPFYLKVKPTRYFTPRTENRFTII
                     GIPVNNTALSMVTSEIIWISTEVEAPDEDTVEDLERIEENISVKICDSDEKMDVAMPK
                     IVTDCKEDGYCDAEGAAELILEKIQELHNVVIDVSKQLNNELAQTNCSMATSQQNGCV
                     ISDLLKPDQLKLFKNIPIYLQNDSLSQRTLEIVLGNTKEATKYPTIKNYAIRENVGST
                     SGGLQMIGNIIHVDNKMQGCGRYKITIDGRIATKDQKTLAPEKAYFFVTIDYNKTGPC
                     AKSIENVRMYLPKDTTHFKKSNMGDTWLTMISGDENIGKTIAKQAFDSDERFQLRTDN
                     IKKFSQLDVSIGTITENENAIAKLYFNNTTTATSPQPEKVNLVVNDYYTINKEGEESK
                     YSEEFVKQVSENVKDIINGTPADICISQDKNYMLILSFEAAEYGALSLKAQNDKLQLT
                     PLEKCVVLTAQSAVAEKITVKYEDILGVRTKFNYSGQDYTDKLSLVLEKDKPTDFNVC
                     FLGDIGQVVSWSDQNVKIIAESGYRTGKINSERKADVNITLINSGMTPIQLIGIGDSV
                     SGKLSSENAGVNEQSFYTFVSWDKEYNKNDKEEYCEALKKYYDSLGGKATMFNKPEEC
                     DFEETKTEKNAKVSRALKRAGSYLLGCFSSCALLTYGADSLWNLIPVLGQVMFVHNVI
                     KDIAMNCGLGCGIPTVLMFGEETGITEKVMDFIYKIPIVGDFAKFLGAALNFLGGAFN
                     SIAGAIFGGGDGVAETEALASQLAEEGAGASDLSTAAGAGVATVGIRDARRLNGFPGD
                     IGLVVPESGVESIKWTDIGGISGLDIDDVGKIIEIPNSEVPRLVNFVPGTTTTTGGGL
                     GRTIHSTKLVNHIDYLAHNPQKGTKYWFNAGRGDQTTVNRILKKAGLHPDNTEFIKMT
                     PEQKRDLFYSMPTEDKQKIADILKKHPNSYIQNPNIIDERVGAHIPSGERFAGDIEKH
                     LKSPLTTTSKITGGNQVQFINSAESTKAELQKLLTKTQAEAAVDKLKNQINEIDDVIA
                     KAREGTTIELDEISKINNQLDD"
     assembly_gap    17766..17865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17866..19615
                     /locus_tag="PLK55_02225"
     CDS             <17866..19615
                     /locus_tag="PLK55_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35775.1"
                     /translation="GTTIELDEISKINNQLDDLDKLLKGTTSISTSTAAITTDDQLKI
                     VNSLKQTNQGIDDGIKQIDDAINITNPSDTAKLDTLKANRESLLKAKEGLSEISDDVS
                     KLKPNGAGSIEVSSELQNKINNVSLIDDQIKGVNSALSSIVTGSKSTMSSAQLANTIS
                     GLEKSSASAAKQLAMLDTQIANMEKSKNIVTIGARNKYLEKLKDQRAGLQQAIEKYKT
                     AANELASAQDDLAKTGKGAIEVSDDVINKIDDASGLVAAQKLPSKLRRISTGLLKMGV
                     DMVIVGISNAAGKEYLNRLSNENGQLANLVYQGIPVTEFEKNVWYKVTVIKNSDRYNI
                     MIEKEIPAISNDIVIYYTDNKEIKDIQTLEHQDELLQNNTPQVQSQDWSELKEEKDRI
                     DATQIATNIEDLQMRMYSEHETKLKDYASCTFSSSGTVCVDIKPLTPNMCAAFVTGAS
                     SKLYGANYVAGNACDSGTGANQNYGTRNRIVWQKGEDSTSSLDNVLVPGVVIGIEGTS
                     NVLKNCQPPHVVLYVGKNEYGQHMILQQNGSKGKLSPLLSADGGAYPMEKIFFIVVPK
                     SGYATYDNFAINNNLS"
     gene            19665..21455
                     /locus_tag="PLK55_02230"
     CDS             19665..21455
                     /locus_tag="PLK55_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35776.1"
                     /translation="MNKKGFTLFTALISLLLVSVALSLIFNMINTEDTYLSLIQDQSS
                     MSDLMTIADLARADAFNTFIITLRSNWEEHMSRNHMLVTRDQMDMNWDDFTQWFIKDN
                     FFERAFPAYFAQGILFNLKYKMEPPGYDIRTETNTGTKIVCTENCEYGAPNCNCEIQA
                     TDPDSDTNNLFIDVVVDIFKAGGEKADIIDCNQTDTNCNGSFFLTLDTTQLADENYEL
                     LPIVTVLRTKSNQVIQRPVLSRQIYKIYMPWRGLQALRTARNIVLDNDTEENENPATH
                     ENVGFFSPTIHNTLEQARLGFCDPGTCVPRNNFFITPNKIGVGNSFCHTIPKTLTASV
                     PSNLLSISQVKNLPTTYDSTENIKDGFLKLYGQSLLGNLSQKRNGQTITYNNGLIMDG
                     LITPIAPDVNIFEFVLDVSSKRSKKYVTTVASNFDDDYGAFNFDALSKMPSLVGGLGL
                     FLKDNKSSLIWQVNKPWYDNQASLNLPADNSQYSGIFNCSEINNATTVLQFIETDPRY
                     RIKESYKGVNTNIKIHLREDFSEFYFTPDNNWSNLKLIINDNGYLDPNSANSFGDTTI
                     DEIGSGIQPWVCYSQVDSDSSAPNCGPKKS"
     gene            21607..>28076
                     /locus_tag="PLK55_02235"
     CDS             21607..>28076
                     /locus_tag="PLK55_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35777.1"
                     /translation="MGLKEFYYKLEDKYFAMLDNLEEKGINLYKLVDPLEKNGIPTFP
                     IFIILILAIIGLIIFLLLPVFGITGGPHGTAVQFVNETNDPISDITLKVKLNGQSKTI
                     ETDSEGYFYFKDLSKDSTYVLKLADDKYLFSDREEYTIDPKKESLVVHLKEGAFSTTK
                     TILFKTQSGEAVTSELNVEFSCLGTDYEKTVIVKGGSHTLDDIPTDCGSLTVDVKNRS
                     DYETKIPADGTSGEIIFSSSVNYATLSVTIKDKDKGIILPGMSVSIYDLTGIKVDSGP
                     TNANGIFSTELVQVSKTYRILVSDDTKKIYSGINDQDYANNIQTPVTITTGINTKSLE
                     IKQDVVGFIKVKVKNDSGLYLEGVSVKLSKGSSTIDIKTTDSTGVVEFGVKENVTYVL
                     SFDKEGYFVYTNRNATVSESTTEVTLRSMTEETKTSLLISVTDTELNPLEYATVKIWD
                     ALNNTVVKIVTTDVTGRVIISNLSVETTYLAEAIVGPFTGRTNNFVVEEREETSVPIL
                     VSIGYGTYNLTILNSDGNPISNATIKAYDASTNKEMTEKQTTTNETGLAILPIRADKE
                     VYFAIVTADGTIITKRYSVDAENVTNDTIVIPSNVSSSASLEFVGYFSANGEQLPAVS
                     AGQSATARFLLNVNKKYSKVLAHIRTGEGETCGNRTYSITEDSIYIKQIKFAGNKIIG
                     SIDYTPCNGESKDTASIVRKDAKWFNVILDNPIEGSYIIDADIVVTDTATTDQSIFYR
                     AEYQQGSAILRNPKDDVIGTSLTSPNRQALYAYANKKIISVGDSSSCENGICYAFTIK
                     DKSTGAEKRIMDKFTAKEGTDYQLNFRLSFERPATDAILVVSPTGQTIVLNEYDITSG
                     GSLPITDTDFSNISLGNINANGTVNGSIDLSVINDVTDELTFNIYANSDSVFSKIIHF
                     DLKPSKKMIVDVVPKTMVPFVPNVISVGVTEESGTKISDATVFIRINGKTIANGKTDR
                     DGLYSVMLPSTDIKDEVEFTVKKQNYSGVTIKQIVSENLVVATPEKLDIQLDISKGYS
                     QDSGLILVNNSVLPLTIIGLETNIRSDYVSIKSGAQGTILEPSFQQQINISTKLTEKG
                     IDLMAQKVIEGDLLISVSEASLAKVWVIKVPIKVRITFGNSVDNVDCLEINPQETTIR
                     VENSNDIEYNLKIKNNCTVDDEPVDIGRIYAESDYKNTKKLGDYFVVVSGKEQKLSLD
                     EKVEVANKIVAEETATIKLIFRPNKSAKSGLSNPIITFSSNRPNVNGIDVIESQHLPS
                     IILNNYVSCIETPSSPITIPYCMGYGYNLYAGGTNTMNFNPFLMGQYNYQYAGQAPQS
                     MNQSWLTGSTYPTSMMGYQSVLGTGVPYTNYRENTPAGYQGAQGFSSSGMFGCEKAQI
                     QIRNNCSEELSLQFSTLGGVIITDPENSEMALKKGASGMLGVQGSQVLGQYKIAVNAK
                     PDDDAIEEYTYVKDIVVSVNRPVSILPENCISVVPTEFDFSGIDTTEKKLMVINTCYD
                     MGYALLGLSVLNEDQLIVGEVQYFSIGDVNANLKPKKTYRYVDGKTTEIMTIKIRRNP
                     DITEKTLRLDSEDNNSIAKKLTALRKDYYDVGKSVNLTALLGITYSNPRLGNDEMEAE
                     ITLTDKLQWLSYIDSFADTSSALEGQTPPTDSTDTTTGAQVPSFTEYFAGSEALNPGE
                     CYDPKGFKKQGFTGEDNFKKYGFNRLLFTYNDTDITHNICDYGRVYCDQDQLRIAISK
                     KADMQGANKTYLLDGTYFLLEGNKIAANPYFFDEDENPLDKAAIESIVSSQTFNNRDP
                     NSILQALKAVLNRVPADLRKFTILEAKIGPITTSEPTPVNLSADNIKAYMTIFGDNDF
                     TKNVKQDGDVYQITVDSFLRSEGKLEEKINSASTSLQEQAIIAEFLNQYLNTMQLYYG
                     DTISPFIINAKTYGALNSGNTKSIFGAVQFMSGAWNLTLDSTSPVKEVATPGTYSIAF
                     KNSSTNKQLTYTVQKITSNLLDYYNNNPDYKTNTFFTNPTNAYYFHYFGVPFKTANTG
                     QSKATVIINNYNNGWDKVQDGIILNVPKDNAAISFKDIRPLQVLSSGAYKIEYIANGK
                     YSSKDTSELKNTLFIFQKINETTKGADYQLKTSSNLDMKFNIVPTTLSATKNFTHVYG
                     VLKDITSSSNFTAEETSSVKIMVDG"
     assembly_gap    28077..28176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28177..28775
                     /locus_tag="PLK55_02240"
     CDS             <28177..28775
                     /locus_tag="PLK55_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35778.1"
                     /translation="SSNFTAEETSSVKIMVDGISSETMCFNFNNGFMLWYNPQKTTFS
                     VDANMATTENATAGTGQGSISPATPTPATGPAQLAITGGNFNVNTPIGSEPAKVTGGN
                     LTLDLENKKLSGTVKFESGNKTAEVSISSGKAIISGNTVQLNISGKALGFDVTASGNL
                     NISGNQGIDGTYTIVSGQKASLTGPVLTLSNLNFTKTA"
     gene            28827..>33819
                     /locus_tag="PLK55_02245"
     CDS             28827..>33819
                     /locus_tag="PLK55_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35779.1"
                     /translation="MGLKKFYYSIENKWYDFVEKTGLYKVTDKIDKFMPSLIFFILLI
                     IMIIAGIFLLLPMIKGSGDIELTFKVMDEFDEPVPLAPVLVTTSDDAYNLKTNENGLT
                     SKIRLPFNTKYSVDIDYFGSDYESYSEEFVAEYDDEIDIYLYSISEETTSTYSFTLVD
                     SQTRMQINKTGTIEFMCSNFGVLPPNSLTFSSGNITIIADSDCQLKAKSIMIEGYVTR
                     NDISITQNIGTIDFVSSDPIDFEAVYPVYVTVLGEDGSPLTGMRAELYTNGIAAYGKN
                     CTTTAGSCTILSVDAGSYVLKITDTRAVPEYGLVEESIIVSGITPKTINMTRTIAGYI
                     QVKVKRTNNQAIENAEVKLKYGDNIISSGHTTNSNGIVNVPVYDLSRTYRVVASKEGY
                     LVGSASNITASESIPTNPTVTITLQQITPQTTATVKVTVLDSVTVKGFSHAQVVLYDS
                     TTGFLTDYDSQITNSDGNCTFRVAPGTYYAKAFKGSSEGVSEEFEVIISSSTEEVNVI
                     IPIEVVKTSINITVVDHYGEPVPRANILLFDKYNSRRAIWGGDLANIDGTFLISDLVS
                     DSEVYAVISDIADNLGATQSEYIYLKADTINELKVTLYPKQTSLQKPEIKFLGFFNDV
                     GEKLNGYLKSGADYKARFLLLIPQDRDGRDEFEEVGAVVRTGSPSSVASPYMENDSIY
                     IRSINLPNAEVTRYTQYSSESGYDGIDDVDTLTEGDAKWAKILFNEIYGSNYANAYTI
                     MADVKVKDTAVYGEEVAINYLGYGYTENNRYETDPIDDTPNSNDLEYMTQKTENFNIG
                     EEQACSDDFCFNLNIINLDEDLREDVVDNYTASPNQHYKLRFGLINNDADTVYNVNSR
                     MQIKNEDKGLKFSSIKITQPNGQLLTTTANENTSEYDISITQFNPHTKILGEIDFIPV
                     LKGDRIFDLTFVSDQRVIFTKQVAIHVLSDKTFIVKITPNVIPSGKNFNLQVEVKDAI
                     TNIEVDKQVNVRVKDRFKRDLLANPVPVGALGIASVNNIPGQEPNNKVYVYVEAQEYE
                     TSISEIKITDEIFEITPTRLGVSLNINTKKTENAKFTIENRSESELVIDSITFMGNDS
                     EIDAIDVQRVNNSLLGFVGTEIPGVDSSEKPGADNYNNKQEITASFFTSPRASTIDST
                     RNISSKLVIKLKDKYNKEYIWATELPVTLTVGYEGLMDDSHCMTLSEESWETTVVDKY
                     VEKQFTFKNNCGVNSKPVPLNGGLSAKVEYEGSSPLGVFTVNVGNRLVELSHGYYKNI
                     YDSVDSEKQHNVVLKYTPVGRFTGDIKGKIIFRSLNDTGSGAQELISEYKFVLHVISL
                     KDCYAVSKKVLTAKDTGEPDAFVIENKGCGSEITYRLSCDDCTGLVLNPKENIVVPAT
                     GSSEEIKVMSQGAMPGIYLLNVYSKVSGARGSERNVGKVRVEVRPTTQCIDLDRYEYD
                     LYRYEFSDTGNPTKAKSYDIGNIINKCYGQDVNVLGKMKKDAIWKMALWNGLRDGLFT
                     WGVSSLIKNFEEVTKNIKSITSSVLATVAKDYTNSTPAQKTDALMNELNKIDKELDKY
                     LKYLDNPKIKSKEKTNIKNAVTDLESGLGKLKAKFPDQAGIAPANALVAEIENKITEI
                     KEKLRDNSKEPECSTTKLCTDTTKECKDGVCVAKSAEPECSTTKLCTDTTKECK"
     assembly_gap    33820..33919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33920..34191
                     /locus_tag="PLK55_02250"
     CDS             <33920..34191
                     /locus_tag="PLK55_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35780.1"
                     /translation="GVCVAKTVTTVYPTYYLASSNNGTRACSEANIQIGQKGCVCVYS
                     GNKKWVVTARCASNNKLYLYKVAASEAVCTSQQTNSSTICGPRKD"
BASE COUNT        13094 a         4750 c         5894 g        10311 t
ORIGIN      
        1 tttaacaaca tattttgtag tcataataat aatagtacca aaaacagtat ttaagtgttt
       61 cgggggtagc tactcttttt ttatttcttt ttctgagtgt ttttagtaat gtcttttgtt
      121 ttctcaagta gtttatcaaa atcagattta aaaagtctgt cttgaaccac tctatatttt
      181 tcaaattccg attctgcatg ttgtttagca atctcttgag taatacttcc agcatttttt
      241 aataattccc tttcatcaaa ttttaaaaaa gcatctagtc tcttagacca atcttccatg
      301 ctcataggta tttttcgttt agccctatct tctgctaaat caagatacgc agacacaatt
      361 ctacttaaag actctagttc ttctttttta agataatttt tagcaacact tacatctgtt
      421 tttattattt ttccagtagg tgcattcttc caagaagtta agcccatatt ttctttttta
      481 gcatctgctc tttttattat taactcagga gcagtatttt gatgtattgc aaaatgcaat
      541 ttattttgaa cttttgaaaa aaaatctcta gtagtttttg cacttttatc ataatctaca
      601 cttgtagaat agatgtctgt aattttctga taaaaatttc tttcactaag tctaatctct
      661 ctaatttcat ctagtaattc attaaaataa tttttattaa aaaaagagcc attttctaat
      721 ctttttctat ccaagacata tccttttatt gcaaattgtt ttagaatctt tgtagcccag
      781 attctaaact gtgttgcctt tttcgaatta accctgtagc caacagaaat aattgcatcc
      841 agattataaa atttagttaa atattttttt ccatcattag cagtatgttc caaaatggaa
      901 ctaactgatt cttcaacaag ttcctgattt tcaaatatat ttttaagatg tttagtaatt
      961 gctggccttt gtactccaaa caacaaagca attttatcct gagtaagcca cacattttca
     1021 ttatgcaaaa atatttcgat ttttacatct cctgtaggag ttgtataaaa taaaaactca
     1081 ttagaagaac tatctttaat aatttcttta tttttcatat ttatcacagg caattacatt
     1141 taactataaa tatattaaat ttagtacttt aaaaaacttt cgggggtagg tgtccaatga
     1201 agtaaaacct ttttaaattc ttttgttgtc tgtttattta taaaatggat ttgatataat
     1261 gaatataatt ttaataatcg taattgttta ttttgtgtta atgcttgggg ttgcatggta
     1321 tttttccaga aaagaaaaca ttgaaactta ttttttaaat aataaaaaaa caggtttgtg
     1381 gttgatggtt ttctcaaacg tagcaacatt aattggggcg ggcgccgtag ttgcaatggt
     1441 ttctgaaggt tacaattcag gtatttctta tggactagca tcaattgttc tttctattgt
     1501 aggaatgata attttagcaa tatttgcaaa aagattaaga caatatagtg agaataataa
     1561 tatttattcg attgcagatt tttatgaaaa aagatttgga ccaaataata aaaagcttct
     1621 aacaatccta caaccaattt taatgattat ttgggttgca gttcaagtta ttgcaatttc
     1681 agcaatggct agttttttaa ttggaataaa ttccactctt gcgattattc ttgcggttgg
     1741 gattacaata atttatacaa ccctaggtgg attgaaaata gatattatct ctgattgcat
     1801 acaattctgg atgattttaa taattttaat tgcaattgca atatttggat attttcaaat
     1861 tgggggatta aatcaattag tactatcagt tccaaaaggt catttgaatc catttgcttt
     1921 tggtggatta agttggttta ttggaatggt tttatttagt ggttttatgt atttgggaaa
     1981 tggagcgcat tggcaaagaa ttttgtctgc aaaaaattca gatgttgcaa agaaatctta
     2041 ttattattca ataccattta tgataataat aagcgtaatt attatttttt taggattatt
     2101 agccgcagtt attttatcta atataaacca agatcaggca atatatttat taatgtataa
     2161 aactttaccg gtatggatgg ttgggttagg atttgcagca atacttgcag tagtaacttc
     2221 atcaattgat agtttaatga ttggagggtc tacaataatt tataaacaat tttttagtac
     2281 aaaagaaaag aataatcttt tgtacgcaag attaataact gttctttttg gaataattag
     2341 tggagttatt gcttttatta ttccaagtat aatcaaatta tctctttttg tttcatatat
     2401 ggcattaatg ttcgtaccat cagttattgc aggtttcgtt tctaagaaaa catctgggaa
     2461 agcaagcttc tattctatat tgctttcggt aatttgttta tctggtttat atttctttac
     2521 aagttacagc aaatccagtt ttatacttgg gctactaatt gcagtaatta caattttatt
     2581 ttatgataaa gttttcaaaa aggacgcaaa taaagctttt taaattcttt tattctttgt
     2641 taatttatga ctatacagtt aaaacaattt actccaggaa aaggagcaat ctattatttt
     2701 tcaggaactg gtaataccgc ttttgtggct ttcgagtttc aagaaaacta taaaaaagca
     2761 aatgtttctg ttgatctctt caaaatagaa aatacagata ttgtttcaag tcaaggcaag
     2821 tatgattttc taatgcttgg ctttccagta tatgcttttt atccgccaga tctagttgtt
     2881 gattttgtaa aaaagttgcc agaagtaaaa gataagcctg ttattctttt tgcaacagca
     2941 ggaataacaa caggtataag ctttaatgtt cttgaaaata ttcttaagaa taaaaactac
     3001 aacgttattt ctaattttgc atatattatg ccagacaacg cctattttgt ttttagaaaa
     3061 gacacagaca aagatgagca gattgagtta tctctaaata acacaatagg gaaaatcaat
     3121 caagattttg atattctaat ttctggcaaa tctaagttta aaaaagcaaa tgtcttttgg
     3181 tctttgtttg ccccattaac taagtttttc ttttttaaaa ttcttcaacc aaagaaaaat
     3241 cgtttcgtat gggacagaga agcctgtaac tattgtgggc tttgtgcaaa gatttgccca
     3301 acaaagaaca taacaataaa gaaagagaaa ggcaaagtaa tctttggtaa caactgcatg
     3361 ttctgtacaa gatgttatca tttctgtcca caaaaggcaa tacacaacaa gtccataaac
     3421 agaacaaaaa aatacagaag atatacaaga ctaaagaatg aaataatttg atggtgaaaa
     3481 tatggaaata ataaactaca gctatgaaga aatagaaaag ctatgtaaag gcatttctaa
     3541 aaaaatagaa aactcaaaat ttaaagtaga ttacatcctt ggagtttctg ttggtggtct
     3601 agttcctgca gtaatctttg cgcgcctttt aaaaaccaag aaaataatga gtgtttctat
     3661 ttcttcttac gagaaaaaaa agcaaaaaga gctaaagctg ttgattgccc cagacaaatc
     3721 tcttcttaaa ggcaagaatg ttttgattgt cgatgatatc tgcgattctg gtaataccct
     3781 tatattttta gaaaatcttt taaagacata caaagtaaag tgcataaggt gtgcaacaat
     3841 ttttctaaac aaagagcatc acaaaattac tccagaatat tatggtaaag ttgtaaccaa
     3901 gtggattgat tttccctggg ataagtttga aaaggaataa ataatgttaa aaagaaatta
     3961 tgtcttgtta tttctaatat tttcaatatt aatctttcca acgatttctg caaagtcttt
     4021 tgcaataaca aattatgata tcaattatac tcttgagcaa tccgggatag tttctgtttc
     4081 tgaaaagtta gattataagt tgtctggctg ttatagagag ctatttcttc aaaggccaaa
     4141 tctagaaatt ctaaatccaa gcggctattg ccaaggcgca gcttgctcct ttgaatacaa
     4201 gcaaacaaac acagaatctg gagaccccga attaatacta aagggcaatt tttgtgatat
     4261 aacagttact gcaaatttta attataatct cgttcatcag attagggctt tagaagacgg
     4321 aacacaattc tattatcaag tttatccagg aaagacaagc gtttcgacaa acaccaaaat
     4381 ttcaatttct tttccaggag atttaaatca aacaacgcgg tttattcatt ctagaaacta
     4441 tcaatcatcg gcaacggaaa gcaagttaac aatttcaaaa tctgttgcag catatgagat
     4501 aattgaagta aacattctaa tgccaaaggc ctggtttata gaagataatc tttatcgtta
     4561 taaagatgta aattatacac aagtccagat aatacaacaa gaacaaaatt ggcaagagga
     4621 atatgataaa tataatcaaa atcaatcaat aacttcatca aggatgaaat acgcaattaa
     4681 taattacgac ataaattata ttttaaacac ctcaggaatt atcgaagttt cagaaaaaat
     4741 agattatact gtaaacgatt gtttaacaca attatctatt caaagaccaa atgatattac
     4801 gaatccatca ggttattgtg ttggtgcaac ttgtaatttt tcttataaaa ctaaaaatac
     4861 attgtcggga tatcccgagt taatattaga tggaaatttt tgtaatataa atgttactgc
     4921 ttattttaat tataatttag aaaaagagat atcaggattg ttagatggca cacaattacg
     4981 ttttgatata tatcgtaata aaatgggggt gcctacaaat attaacattt ctatgttttt
     5041 ccctggagat gcaaataagg ttacaacttt tttttaccct tcagattatc ttgccaaaac
     5101 agatggaaat gttttattgt atgagcgggc agtaaatgaa tttgatactg tatatcttct
     5161 ggcagtaatg ccaaaagaat ggtttgatca gaataacatt tattatcaat ctaattacac
     5221 tgtttctcaa ataatagagg cagaggaaca aaaattaaaa ctgcaggaag aacaaagatt
     5281 aaaaatcgaa gaagcaagat tgaaacaaat agaagaacaa aggttaattc ttgaagaaca
     5341 aagattaaac caatatagca aagtcaacca aaattttatg ccgggcataa aatcacaaga
     5401 accaaacact gattggttat taaatttctt attgtatagt gggattttag tagttatatt
     5461 actaattatc tggttcatat ttggaagaga ttattctcca gaacaagtaa attatcttgg
     5521 gatatatgaa agagatttac cgggcgatga tgatccatta aaggcccatt atttaattac
     5581 cggtgaattt tcaaaagatt ggttttcttc tgcgatcttg tatttagtat ggaaaaaaca
     5641 atataatcta gaaaaaagaa aagacaatta ttttttaata agaaataaat caataaaatt
     5701 gggaaattta ccaagttata taatgaagat agataaattt cttaataaac attatcctga
     5761 aggagaaatc gatttagatt tattaaaaaa aagattaaaa agtcaatctc tcttttcaga
     5821 agattataga caaaattata aaaaaataat tgctcaaggt tatgatttta ctgtactata
     5881 tcaaaagata tatacagaat atgacagttg gtttaataaa aaaagcaaat tatttgatca
     5941 aaaaggatat ataattgcaa catctatttt tagtatattt attgtttttt cattcttttt
     6001 taatagcgca atatttgtca tgtctattat agtaacaatt cttggttcag taatattatt
     6061 gttaattcca cgccttttta aatttagatt aatatttggg aaattttcaa aagagggcag
     6121 aataaaaaat cttcaatgga ctaatttcaa aaagtatatt actgattttt ctttaatgag
     6181 agcgcaccct ccacaacatg ttattctttg ggaagaatat atggtttatg caactgcttt
     6241 tggagtcgct aaaaaaacct ctaaagtttt agaaatagtt atgccacaag aaattagatc
     6301 caatcagatg gttgccactt attcggggtt ttcatctata tcctctgatt tctcaggagt
     6361 tggtggtttt tctggttcag ggggttctgc tggtggtggc ggaggctttg gcggcggtgg
     6421 cggcggaggc tttggcggtg gtggtggagg cggcggtggc gccggtgctc gctaaaccaa
     6481 attatttata aatcctttat tcttatttat ttctaaatat atttataaaa ttaggagaaa
     6541 gaaaaaacat ggtttctaaa gaaaaaaaat ttataatgtg gtttgatgag attggaatag
     6601 aggatattcc ttatgttgga ggtaagaatg catctcttgg tgagatgtac caaaatctag
     6661 gaaaaaaagg agttaaagtt ccttttggct ttgcaatcac agcttatgct tataggtata
     6721 ttcttgagaa atcaggtcta aaatcagaga ttaagaaaac tctaaaagat ttagataccc
     6781 acaacatgga ttctctagct gaaaaaggac acaaagttag acatttaata agacatgcag
     6841 atattccaaa agatctagag aaagaaatct ttgcagctta tgataagtta tgtaaaatag
     6901 aaaaaataaa taatctagac gttgctgttc gttcaagtgc aactgcagaa gatcttcctg
     6961 acgcatcatt tgctggacaa caagaaagct atcttaatat tagaggaaaa gaggatttat
     7021 tagaagcagt aagaaaatgc tttgcttctc tttttacaaa tagggcaata tcttacagag
     7081 aagacaagca ctttgatcat tttagtattg ctctttctgt tgttgttcaa agaatggtta
     7141 gaagtgattt agcgtcttca ggtattatgt tttcaattga cacagaaaca ggttttagag
     7201 atgttgtaat aatcaattca acttatggtc ttggagaaaa cattgttcaa ggtgcagtta
     7261 atcctgatga gtttataatc tttaaaccaa ctttaaagca aaataaaaaa ccaataatct
     7321 caaaagtgct tggagaaaaa gcaataagaa tggtttatga tacacaaggt aacaagccaa
     7381 ccaaaaacct ttcagttgaa ccaaacgaca gaaacaagtt ttcaataacc gatgaacagg
     7441 cattaaagct tgcaaaatgg gcttgcgata ttgaggatta ttattctaaa aaatcaggaa
     7501 agtttaaacc gatggatatg gaatgggctc ttgatggtaa aacaaacgag ttatttattg
     7561 ttcaagcaag gccagaaact gtgcagtcaa gaaaagattt aaacagctac acaacctacg
     7621 tcattaacac aacagctaag ccagtagttg ttggtaaaag tgtaggtcaa caaatctcaa
     7681 caggtaaagt aaatattatt aaagacgcaa aagagattaa taaatttaaa aaaggagaaa
     7741 ttctagtaac agaaatgaca gaccctgact gggagccaat catgaagatt gcttctggaa
     7801 taattacaaa caaaggtgga agaacctgtc atgccgcaat aatctcaaga gagctgggta
     7861 ttccttgtat tgtagggaca cttgatggga caacaaaact aaaagccgga aaaaacataa
     7921 caatggactg ttcaagcgga gatgctggtt ttgtttatga aggcaaagtt ccatttacaa
     7981 aacaaacagt agatctaaaa gaactacaaa aaacaaaaac acaattaatg atgaatgttg
     8041 gtaaccctga acaagctttc gatttaagtt tcttgccaaa cgaaggagtg ggtctagcaa
     8101 gagaggagtt tattatcaac tcttggatac agattcatcc tcttgctctt atcaattatt
     8161 caaaaataaa agacgcagaa acaaaagaaa agattgatgc tttaactgtt ggttataaag
     8221 acaagaaaca attttttgtt gataaactag cacaaggtat tgcaagaatt gcagcggcct
     8281 tctatccaaa gaaagtaatt ctaagattat cagactttaa atcaaacgag tatgctaacc
     8341 ttattggtgg caaagaatat gaaccagaag aaagcaatcc aatgataggt tggagaggtg
     8401 cttcaagata ttaccaagga aactatgttg ctggttttgc tttagagtgt caggcaatta
     8461 aaaaagttag agaagatttt ggtttaacca atctagaatt aatggttcct ttctgtagaa
     8521 cacttgacga agcaaacaaa gttcttgcag aaatgaaaaa gaacggtctt gagaaaggca
     8581 aaaatggttt aaaggtaaac tgtatgtgtg agattccatc aaacgttatt cttgcagaca
     8641 aatttttgga gatctttgac ggttacagca ttggaagtaa tgacttgaca caattgattt
     8701 taggccttga tagagattcc gagttattgg ggaatatcta cgacgaaaga aacgaagcag
     8761 taaaaaagat gttaacagac gtcataaaga tagcaaacaa gaaaggcaaa tatattggta
     8821 tctgcggaca agcgccatct gattatccag agatggcaga attcctagta aaagaaggaa
     8881 taaaatccat gagtctaaat ccagacacaa ttataaaaac aagaatggca atagcaaaac
     8941 ttgaaaagtc tttaaagaaa taatttaaat tatttctttt atttttttat ttagaaaaat
     9001 atgaaatcaa tactttttgt ttgcacagga aacatcttta gaagcatgtc tgctgagctt
     9061 tttctaaaaa aatatcttct ggaaagaaac aaaaaaataa aagtgtcttc agcaggcatt
     9121 caagcaaatc cagaaggcat tcatccaatt gttaaggaag aactagacaa gagagaaatt
     9181 gactattcaa aacacgtgca aagaaaagtt acaaaagaaa ttcttgattc ttctgatctt
     9241 gttgtttcaa tgacttattt tcatcaggat tttttaaaag aaaaattcaa tcaagattcc
     9301 cttcttttta atgagctttg ctttggtaaa aaagaagact ttctagatta ccctgacatt
     9361 ctaatagaaa caaactcaaa agatagagca gaagaatata taagaacagc aataaaagaa
     9421 atagaaaaag gctgcaaaag tcttgcaaag caactgcaat aatttcaaac aatttcttta
     9481 taaacattaa cactcaatat atatctagtt ataataacaa aaggtgatta aaaaatggca
     9541 atagcactaa taataattct agttattcta ggtgtaattt tgctaatggc tttattcatg
     9601 tacaatagcc tagttgttat gagaaacaag gtaaaaaacg gctggagtca gattgatgtt
     9661 cagttaaaga aaagatatga tcttgttcca aaccttgttg aaacagttaa aggctatgca
     9721 aagcatgaag caggcacatt atcaaagatt acagaattaa gaaatcaagc tatgcaaaca
     9781 aaggacataa aggcagcaat gaagactaac gaccaaatgt ctaaagcaat cagcggtcta
     9841 ataagcgttg ttgttgaaaa ctatcctgac cttaaagcaa gccaaaattt cttaatgctt
     9901 caagaggaac tttcaggtat tgagaacaaa atagcatatg caaggcaata ttacaacgac
     9961 acaataatgg attacaacaa caagttacag gtctttccaa acagcatctt tgcaaaaatg
    10021 tttaatttca aagaagaaac tttcctagaa gcgacagcag aagaaaaaaa gaatgtaaaa
    10081 gtttccttct agtttcttct tctatttttt ttattgtttt catttgtaac tttttctagt
    10141 aaatagatag tatttaaatt ctttttcatt ctttgtaatt aatgaaagat atataaatat
    10201 atattgcttt tatttatata ggataaaata tattttaaaa tattaaagaa agaaaataaa
    10261 tggcaaaaga aaaagcaaaa cctaaaggct ctaaagccaa aaaagaagaa acagattttg
    10321 aggatacaga aattgatgat tattctgaag ttgatgaaga gttctcagaa gaaaatatag
    10381 gcaagcaaac agagaataac atctttggca aagcctacaa gtggttagaa acaaaatatt
    10441 attctttctc tgattggcta acaaagaaag gaattaatct taataaggca aatgatttct
    10501 tagaagaaaa aggaattcct gcctttgtgt ttgttgcaag tcttacactg attattttag
    10561 cggtcctaat ttttttaata ataaattttg caacaaagac aacagttgaa ttttcaatag
    10621 ttgatttttc taataatcca ttatcagatg tagatgttaa aatctatgat ttaaaagaca
    10681 aaacaaagtt taatggaaca ataagtgacg gccacaaagc aagactttct cttaagtcag
    10741 gaataaatta cacaataaat gccacaaaat caggatttgc agattatgaa aaagataatt
    10801 tatttactag aggagagcca ataaaaataa aattctctga aaatgtaact cttggcaatt
    10861 ttaaattatc agtaacagat ttagaaacta aaaaagcaat ttctaattat agggcaattt
    10921 taagttatga tattagtgga aaaccgcaag aacaaacagc aactcccgga agtgcaaatc
    10981 aaataagttt tacagatgtt cccttaggca aatctttgtc attagttatt gaagcagatg
    11041 gttatcaaac agtttctaaa tctataacgg tgtcaaataa tgaagaaata ataccgattg
    11101 aattacaatt tgatcttgat tctgcgcaat tagaaggact tgatgcaagg ggcacaattg
    11161 tagcagtttc agacactgga gatgtcttag atgattgtga agttacaatt tataatatct
    11221 ctgacgaaaa aatagcaaca ggcataactc aactaggcag atttacattt actgaaaaag
    11281 taggttcagt aataagattt gttgtaacaa aagaaggtta taaaacatac gacagtgatg
    11341 aagaaaataa aactttcaga ttactaagaa gtgaagacac ttttactgca acattaaaaa
    11401 aaggcagttc tgatttaaaa gtaatggttg tagaagatat ggttggtcca gtaagtgatg
    11461 cagatgtttc tttatataat ctagtaggga acttattgac agaaaaaaca accttgcttg
    11521 atggagaagt tatattttct ggtcttgata aaaacaaaga ttatattgta acagcatgta
    11581 aagaagatta tctttgctct caatattttg taaatataga taatggtaat gatttagagg
    11641 caaagcttgt taagttgggt tctgcagaaa cttacaaatt atcaatatat gtttatgatg
    11701 gtgcaaacgc cccactttca acagcaaatg ttttagttta taaattatta aatggaaaat
    11761 atgtgccaat cggttttggg ccattacaag ttgatttaac aggctacaca tctgtcttta
    11821 cacaaaaggc agaaacatat aaagttgtag gcattgccgg ggattcaaat gcttctcaag
    11881 aaatcacaat tgatcagttt aaagataaca aagtaatatt gataataaat gatgcatcaa
    11941 aagaagtaac cttacaatta aaagacattt atggcaaccc agtaattgat ggttatgtaa
    12001 tagttaaatc aaaaaccgga gaaattcttt acgaaggtta tatcgatgca gaaaatcctg
    12061 ctaaattttc aacaaaaggc tacaaagatt tgattgtaga atatctggat gaagaaggca
    12121 ataccacggt tgcttctgca gtagtgggat ctggagaaga tttagttgta agcttaaaac
    12181 caatgattga ggattttaat ccaataattt ctttcttaga aataagagat ttacaaaaca
    12241 attctacaag tatattaagt aaagataaag attattattt agtgtttgat ttacaattgc
    12301 cagtagatgt gaaaaaatgt ggagttcatt ttagagcagg aaaagattca gaaaatgatt
    12361 ctgaaaatat ggattatggt ataactggat ttaaagcaga tacaactagc tttaaatatt
    12421 ccacaacata taatgaaggg cagcaatcaa ttgattttga taatgttggt tctccaaatg
    12481 aattaaataa atggttagaa ttgtattggc cagatgtgtc attaacaaat aaacaaataa
    12541 tgttaaaagt taaagctaca gaaattgggg atttgcttct aagatataga gcgtggtgtg
    12601 aagatagcga aggtaaaatc tatagagatc cagaagatac aacattagga actgccagaa
    12661 acaattcttc aaaacaaagc ctatatgcag aaacaaaaga acaagtattt aaagtttttg
    12721 aaactcctgc agaatgttca gacaatctgt gtgtagaata taaattttta gataaaggcg
    12781 gttttgatta ttctaatgaa actttttttg gaacagttga tgaaatgtat ttattagaat
    12841 taaattacta tagtccaaaa tccggagaat taacaattga tactgaaaca gatgcaagtc
    12901 acccaattat tggtttaata aattatcaag acacagattt gtttccaagt tcagaacagg
    12961 attctcaaga aacaaaatta agttctatgg ctgtttcttt acaatcgggg atttatagaa
    13021 aacaatattt attatttaat gcaaaaaata taggtgtaac ttatatagat ataaaatcaa
    13081 cctttaattc taatgttaca gaaaaaagat taacctttga aataaagaat aaaagaaatt
    13141 tagaagtaaa tgtttcagag ttattaccat ataacacacc aataacaata gaagtaaaag
    13201 atgcagataa caaaagccca atcaacaacg cacttgtaaa attagaagac gaatttggag
    13261 atcttatttc aagtacaaaa aaagcaaagg caggaaaata tataattaat caaaactttt
    13321 ctagttcaaa accaaattta gaagttatgg cgcctggtta tgcaccatac actcagcaat
    13381 taatgattgc agatactggt ttaatttcag gtcctgatag tatttctata aactttggta
    13441 atgaaattag tcaagaaaca gaaataataa ctttagtaaa taaaggaaat gcaaagatta
    13501 cggatttgac atatcaaatt acaccaattg atgaggttgc agaattaact atagagactc
    13561 aattgccagt aacacttaat ccagcaagcc cttcagaaat agaagtgact tcagtaattg
    13621 atccaaaact aaaatttaaa actgcaaaat atgttttaac aatatttggt tttgttggca
    13681 acaaacaagt tgcaaaagat attgagattg aaatttataa ggggcaagta caagaaaatt
    13741 gtttagatat aaaacctctt gcaatttata gttatgtagg cattagtgag ggttctgaaa
    13801 atgagataac tgcaacgtta gttaacaatt gtaaaaaagc aattacagtt actccagaac
    13861 tattaaaagc aaaaggcaca gtaattaaga aagatgaaaa tttagaaatt gttcttccat
    13921 cagtaacaat agaaccaaat gaagaaataa cagattacac aataactata aaaaacaaca
    13981 aggctcgaaa gactacaaaa agctatattt ttgagattgc ttggagaaat gcgtattatg
    14041 ctttaccaaa tacaaaatta aatgttgact tagttgatta ttctaagacc ataaagatta
    14101 ttccaccagt ttcaattgta tcaatgagtc aatttaatga agagatagtt gcttcaaata
    14161 aaacggtttt ttcaataaca aataccggca aatacccctt aaaggacata caaatatcaa
    14221 ggttggaaga aaaatatgtt tctaatattc aagataagat tgaaccaata acttttgaag
    14281 aaataaaacc tggccaaaca aaacaagtta gtattattta tgaaggcaaa gtgagcaatg
    14341 caacaagagc gacacttttc tataaagtga ctgcaatttc tcctggttca aaggatccaa
    14401 tagatgcaaa atttactgta gattttagaa tttcttcagc aagttgttta aaaatagatc
    14461 aaaaaaagat tgcgttctat actagaattg gagaagaaaa aatcaagtta attaatataa
    14521 caaatcaatg tgcagagcca atagcatata tagattatga tataaatcct aatagggcac
    14581 ctaatgattt tactttggca tttggcgaag gcacaggtgt tgctttagtt cctgcaaccc
    14641 caataccatt tataggtatt ggtcaaactg ttcctttcta tttgaaagta aaaccaacca
    14701 gatattttac tcctagaacg gagaatagat ttacaataat aggaattcca gtaaataaca
    14761 ccgcattaag tatggtgact tctgaaatta tttggatttc aacagaagta gaagcacctg
    14821 atgaagacac tgtagaagat ttagagagaa tagaagaaaa tatttcagta aaaatttgtg
    14881 attcagatga aaaaatggat gttgcaatgc ctaagattgt tacagattgt aaagaagatg
    14941 gttattgtga tgcagagggt gcagctgaat taatattaga aaaaattcaa gaactacata
    15001 atgttgtcat agatgtttca aaacaattaa ataatgaact tgcacaaaca aattgttcta
    15061 tggctacatc acagcaaaat ggctgtgtaa tttctgattt attaaagcca gaccagttaa
    15121 agttatttaa gaacattcca atctatctac aaaacgattc actatctcaa agaaccttag
    15181 aaattgtttt gggcaacact aaagaggcaa caaaatatcc aaccattaaa aattatgcaa
    15241 taagagaaaa cgtaggttca acttctggtg gtttacagat gattggaaac atcatacatg
    15301 tagacaataa aatgcagggt tgtggaagat ataaaataac aatagatggg agaattgcaa
    15361 caaaggatca aaaaaccctg gctccagaga aagcgtattt ctttgtaact atagattata
    15421 ataaaacagg cccttgtgca aaaagtatag aaaatgtaag aatgtattta ccaaaagata
    15481 caacgcattt taaaaaatca aatatgggcg acacctggct aacaatgatt agtggcgatg
    15541 aaaacattgg caaaacgatt gcaaaacaag cattcgatag cgatgaaaga ttccaattaa
    15601 ggacagacaa cattaaaaag tttagtcaat tagatgtttc aattggcaca attacagaaa
    15661 atgaaaatgc aattgcaaaa ttgtatttta ataatacaac aacggcaacc agtcctcagc
    15721 cagaaaaggt taatttagtt gtaaatgatt attatacaat aaataaagaa ggagaagaat
    15781 caaaatattc agaagaattt gtaaagcaag tttctgaaaa tgtaaaagac ataattaatg
    15841 gaactcctgc agacatatgt atttcacaag ataaaaacta tatgttaatt ttatcttttg
    15901 aagccgcaga atatggagct ttatctttaa aagcgcaaaa tgataaatta caattaacac
    15961 ctttagaaaa atgtgttgtt cttactgcac aatcagcagt agctgagaaa ataactgtta
    16021 agtatgaaga tattttaggc gtaagaacta aatttaatta ttcaggacaa gactatacag
    16081 ataaattgtc gctagttctt gaaaaagaca agccaacaga ttttaatgtt tgtttcttag
    16141 gagatattgg gcaagtagtt agctggtctg atcagaatgt aaaaataatt gctgagagcg
    16201 gttataggac tggaaagata aattctgaaa gaaaagcaga cgtaaatatt actttaataa
    16261 attcaggaat gactccaata caattaatag gaattggaga ttctgtatct ggaaagttaa
    16321 gttcagaaaa tgcaggagtt aacgaacagt cattttatac ttttgtttca tgggataaag
    16381 aatacaataa aaatgacaaa gaagaatatt gtgaagctct aaagaaatat tatgatagtt
    16441 taggtggcaa agccacaatg tttaacaaac cagaagagtg tgattttgaa gaaacaaaga
    16501 cagaaaagaa cgcaaaagtt agtagggctc taaaaagagc aggttcttat ttgctcggtt
    16561 gtttttctag ttgtgctctt ttaacttatg gggcagattc attgtggaat ttaattccag
    16621 ttttagggca agtaatgttt gtacacaatg taataaaaga tattgcaatg aattgtggtc
    16681 ttgggtgtgg tattccaaca gtactaatgt ttggtgaaga aaccggaatt acagaaaaag
    16741 tgatggattt tatttataaa attccaattg ttggagattt tgcaaaattt ttgggcgccg
    16801 ctttaaattt tttaggtggt gcatttaata gtatagccgg agcaatattt ggaggaggag
    16861 acggtgttgc agaaactgaa gctttagcca gtcaattagc agaagaaggc gcaggagcat
    16921 cagatttaag tacggctgct ggtgctggag tggcaacagt aggtattagg gatgcaagaa
    16981 gattaaatgg ctttcctgga gatataggtt tagttgttcc agagtcaggt gtcgaatcaa
    17041 taaagtggac agatataggt ggaatttctg gattagatat agatgatgta ggaaaaataa
    17101 tagaaattcc taattcggaa gttccaagac ttgtgaattt tgtcccaggt actacaacta
    17161 caactggcgg cggattaggt agaacaatac attcaactaa attagtaaac catatagact
    17221 atttagctca taatccacaa aagggtacta aatattggtt taatgcagga agaggggatc
    17281 aaacaacagt taataggatt ttaaaaaaag ctgggcttca tccagataat acagaattta
    17341 ttaaaatgac accagaacaa aaaagagatt tgttttatag tatgcctact gaggataaac
    17401 agaaaattgc agacattcta aaaaaacatc cgaactctta tatacaaaat ccaaatatta
    17461 tagacgaaag agtcggggct catataccaa gtggagagag atttgcaggt gatattgaaa
    17521 agcaccttaa atctccatta acaacaacca gcaaaattac tggtggtaat caagttcagt
    17581 ttataaatag tgctgaaagt acaaaagccg aattgcaaaa attattaaca aaaacacaag
    17641 cagaagcagc agtagataaa ttaaaaaacc agattaatga aatcgatgat gttattgcta
    17701 aggctcgtga aggcacaaca attgaattag acgaaattag caaaataaat aatcaattag
    17761 acgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggca caacaattga
    17881 attagacgaa attagcaaaa taaataatca attagacgat cttgataaac tattaaaagg
    17941 gacaacatca atatccacta gcacagccgc aataaccacg gatgatcagc tgaaaattgt
    18001 taattcttta aaacaaacaa atcaaggtat tgatgacggt ataaaacaaa tagatgacgc
    18061 aatcaatatt acaaacccat ctgatactgc aaaattagat acgttaaaag ccaatagaga
    18121 aagcttactt aaagcaaaag aagggctatc tgaaattagt gatgacgttt ctaaattaaa
    18181 accaaacgga gcagggagta tagaggtttc ttccgaatta caaaacaaaa taaataatgt
    18241 atctcttatt gatgatcaga ttaaaggtgt aaattcggcc ttatcttcaa tagtcactgg
    18301 atcaaaatca actatgagtt ccgcacaatt agcaaatacg atttccggtt tagaaaaatc
    18361 atcagcaagt gctgcaaagc aattggctat gttagatacc caaatagcga atatggaaaa
    18421 aagtaaaaac atagtaacaa taggcgcaag aaataaatat ttagaaaaat taaaagatca
    18481 aagagctggt cttcaacagg ctatagaaaa atacaaaact gctgctaatg aattggcaag
    18541 cgcacaagat gatttagcaa aaaccggaaa aggggccata gaagtatccg atgacgtaat
    18601 aaataaaata gatgatgctt caggactagt tgctgcccag aagttgcctt caaaattaag
    18661 aaggatatca acaggtctac taaaaatggg ggtagatatg gttatagttg gaatttcaaa
    18721 tgctgcaggg aaagaatatt taaatcgtct ttcaaacgag aacggacaat tagcaaattt
    18781 agtttatcaa ggaattccag ttactgaatt tgaaaagaat gtgtggtata aagtaacagt
    18841 aataaaaaat tcagaccgtt ataatataat gattgaaaaa gaaataccgg caataagtaa
    18901 tgatatagta atatattata cagataataa agaaattaaa gacatacaga ctttagagca
    18961 tcaagatgaa ttattacaaa ataatacacc gcaagtacaa tcacaagatt ggtcagaact
    19021 aaaagaagag aaagacagaa ttgatgcaac tcaaattgca acaaatattg aagatttaca
    19081 aatgagaatg tattctgaac acgaaacaaa attaaaagat tatgcaagct gtacatttag
    19141 tagtagtggc actgtgtgtg ttgatattaa gccattaact ccaaacatgt gtgctgcatt
    19201 tgttacagga gcatctagta agctatatgg cgcaaactat gttgcaggaa atgcttgtga
    19261 ttctggtact ggcgcaaatc aaaattatgg aacaagaaac agaattgttt ggcaaaaagg
    19321 agaagatagt acaagttcat tagataatgt attagtgcct ggtgtagtaa taggcataga
    19381 aggcacaagt aacgttctta aaaattgtca accaccacat gttgttttat atgttggtaa
    19441 aaacgaatat ggtcaacaca tgatattaca acaaaatgga agtaaaggaa aattaagtcc
    19501 tttattatct gcagatggtg gcgcctatcc tatggagaaa atatttttca tagttgttcc
    19561 aaaaagtggt tatgcaactt atgataattt tgcaataaat aataatttat cataatacta
    19621 acctctttta aatctttttc ttatattttt atttagattt aatcatgaat aaaaaaggct
    19681 ttacattatt tacagcactg atctctttgt tattagtatc tgtagccttg tctttaattt
    19741 ttaatatgat taatacagaa gacacgtatc tctctcttat tcaagatcaa tcgtcaatgt
    19801 ctgatcttat gactattgct gatcttgcaa gagctgatgc atttaatact ttcataataa
    19861 ctcttagatc caattgggaa gagcacatgt ctagaaatca tatgctagta actagagacc
    19921 aaatggatat gaattgggat gattttacac aatggtttat aaaagacaat ttttttgaaa
    19981 gagcatttcc tgcatacttt gctcaaggta tactttttaa tcttaaatat aaaatggagc
    20041 caccaggata tgatataaga acagaaacaa acaccggcac aaaaatagtt tgtaccgaaa
    20101 attgtgaata tggagcacca aattgtaatt gtgaaattca agcaactgac cctgatagcg
    20161 atacaaacaa tttatttatt gatgtagttg ttgatatttt taaagcagga ggagagaaag
    20221 cagatattat tgattgtaat caaaccgata ccaactgtaa tggctctttc tttttaacat
    20281 tagacactac tcagcttgca gatgaaaact atgaattgtt gccgatagta actgttttaa
    20341 gaactaaaag taatcaagta atacaaagac cagtattaag cagacagatt tataagatat
    20401 atatgccatg gcgtggtctt caagcattaa gaactgcaag aaatattgtt ttagataatg
    20461 atacagaaga aaatgaaaat cctgcaacac acgagaatgt tggttttttc tctccaacaa
    20521 ttcacaacac cctagagcag gcaaggcttg gtttttgtga tccaggaacc tgcgttccaa
    20581 gaaataattt ctttatcacg ccaaataaaa taggagtagg gaacagtttt tgccatacta
    20641 ttcccaaaac tttaacggca tcggtgcctt ctaatttact ttcaatatca caagtaaaaa
    20701 atcttcctac aacatatgat tctacagaaa acattaaaga tggctttcta aaactttatg
    20761 gtcaatcgct tctaggaaat ttgagccaaa aaagaaacgg ccaaacaatt acttacaata
    20821 atggattaat aatggatggc ctcataactc caatagctcc agatgttaat atttttgaat
    20881 ttgtattaga cgttagttca aaaagaagta aaaaatatgt tactactgtt gcaagtaatt
    20941 ttgacgacga ttatggtgca ttcaactttg atgcattaag caaaatgcct tctcttgtag
    21001 gcggcttagg tctattctta aaagataaca aatcctcgct aatctggcaa gttaataaac
    21061 cttggtacga taatcaagca agcttaaatt tgcccgcaga taactcacaa tattctggca
    21121 tttttaattg ctctgaaata aataatgcaa caacagttct gcaatttata gaaacagatc
    21181 caagatatag aataaaagaa tcttataaag gcgttaatac aaatattaaa atacatttac
    21241 gtgaagattt tagtgagttt tattttactc cggacaataa ttggtcaaat ctgaagctta
    21301 taataaatga taatggttat ttagatccaa attctgcaaa ttcttttggt gacaccacaa
    21361 ttgatgaaat cggctcagga atacaaccat gggtttgtta cagccaagtc gatagtgaca
    21421 gttcagcccc aaattgtggc ccaaagaaat cataaagtta taacttatac cagttaatta
    21481 atattatttt ataaaccttt ttgttattag atatattatc caaaaaagat aatatttata
    21541 aacaattgtt taatctatta taatatatat tatattaaaa tctgaaataa aagaaggttt
    21601 tcaaaaatgg gattaaaaga attctactat aaattagaag ataaatattt tgcaatgctt
    21661 gataatctgg aggaaaaggg aattaattta tacaaattag ttgatcctct agaaaaaaat
    21721 ggaataccaa cattccctat ttttataatt cttattcttg caataatcgg tttgataatt
    21781 ttcttattac ttcctgtttt tggaataaca ggtggaccgc acggaactgc tgttcaattt
    21841 gtaaatgaaa caaatgatcc aatttctgat ataaccttaa aagtaaaatt aaatggccag
    21901 tctaaaacaa tagaaacaga ttctgaaggt tatttttatt ttaaagattt aagtaaagat
    21961 tcaacttatg ttttaaaatt agcagacgat aaatatctat tctcagatag agaagaatat
    22021 acaattgatc caaaaaagga atcactagta gttcatttaa aagaaggcgc attttcaacc
    22081 acaaaaacaa ttttatttaa aacacaatca ggagaagcag taacttctga attaaatgta
    22141 gaatttagtt gtcttggaac agactatgaa aaaacagtaa tagttaaagg tggatctcac
    22201 acattagacg atataccaac agattgtggc agtttaacag tagatgtaaa aaacagaagt
    22261 gattatgaaa caaagattcc tgcggatgga acaagtgggg aaataatatt tagtagttct
    22321 gtaaactacg caacattatc ggtaacaata aaagataaag acaaaggaat aattctaccc
    22381 ggcatgtcag tatcaatata tgatttaaca gggataaagg ttgatagtgg accaacaaat
    22441 gcaaatggta ttttttcaac agagttagtt caagtttcaa agacctatag aatattagtt
    22501 tctgatgata caaaaaaaat ttattctgga ataaacgatc aagattatgc aaataatatt
    22561 caaacaccag taacaataac tacagggata aacactaaat cattagaaat aaaacaagat
    22621 gttgttgggt ttataaaagt aaaagtaaaa aacgattctg gattatatct agaaggcgtt
    22681 tctgtaaaat tatcaaaagg cagttctaca attgatatta aaactactga ttctacgggg
    22741 gttgtagagt ttggagtaaa agagaatgta acttatgttt tgtcctttga taaagaaggt
    22801 tattttgttt acacaaacag aaatgcaaca gtttctgaaa gtacaacaga ggtcacttta
    22861 aggagtatga ctgaagaaac aaaaaccagt ttattgatta gcgtaaccga tacagaatta
    22921 aatcctttag aatatgcgac tgtaaaaata tgggatgcat taaataatac agttgttaaa
    22981 attgtaacta cagatgtaac aggcagagta attatttcaa atttaagtgt ggaaacaact
    23041 tatttagcag aagcaatagt tggtccattt actggtagaa caaataattt tgtagtagag
    23101 gaaagagaag agacgtctgt tccaatttta gtttcaattg gttatggcac ttataattta
    23161 acaatattaa attcagatgg caatcctatt tcaaatgcaa caataaaagc atatgatgca
    23221 agcacaaata aagaaatgac agaaaagcaa acaaccacta acgaaacagg tcttgcaata
    23281 ttaccaatta gagcagataa agaagtatat tttgctatag tcacagcaga cggcacaata
    23341 attacaaaaa gatattctgt agatgcagaa aatgtaacaa acgatacaat agtcattcct
    23401 tctaatgttt caagctctgc aagtctggag tttgttggct atttttccgc aaatggagaa
    23461 cagttgccag cagttagtgc tggtcaatcc gcaacagcaa gatttttatt aaatgttaat
    23521 aaaaaatact caaaagttct agcacacata agaacaggag aaggagaaac ttgtggtaac
    23581 agaacatatt caataactga agattctata tatattaaac aaataaaatt tgcaggaaat
    23641 aaaatcattg gttcaattga ttatacacca tgtaatggag aatcaaaaga tactgcttca
    23701 atagtccgaa aagatgctaa atggtttaat gttattttag ataacccaat agaagggtct
    23761 tatattattg atgcagacat tgtagttaca gatacagcaa caaccgatca atccatattt
    23821 tatagagcag aatatcaaca aggttctgca atattaagaa atccaaaaga cgatgttatt
    23881 ggaacaagcc taacttctcc aaatagacag gcactttatg catatgcaaa taagaaaatc
    23941 atttcagtag gtgattcatc cagttgtgaa aacggaatat gctatgcttt tactatcaaa
    24001 gacaaatcta caggtgcaga aaaaagaata atggacaagt ttacagcaaa agaaggaaca
    24061 gattatcaac ttaattttag attaagtttt gaaagacctg cgacagatgc aattttagtg
    24121 gtttcaccaa caggccaaac aatagtatta aacgaatatg atataacttc tggtggcagt
    24181 ctaccaataa cagacactga tttttcaaat attagtttag gaaatataaa tgcgaacggc
    24241 acagtaaatg gttcaattga tttaagtgta attaatgacg taacagacga attgaccttt
    24301 aatatttatg caaatagcga cagcgtattc tcaaagataa tacactttga tcttaagcct
    24361 tcaaaaaaaa tgattgttga tgttgttcca aaaacaatgg ttccatttgt gcctaatgtt
    24421 atttctgtag gagtaacaga agaatctggc acaaaaattt ctgatgcaac agtttttata
    24481 agaataaacg gaaagactat agcaaatggt aaaaccgaca gagacggttt atattcagta
    24541 atgttaccat ccacagatat aaaagacgaa gtagaattta cagttaaaaa acaaaattat
    24601 agtggagtta caataaagca aatagtttct gaaaatctag ttgttgcaac accagagaaa
    24661 ttagatatac aattagatat ttctaaaggt tattctcaag attctggttt gatattagtg
    24721 aataattcag ttttaccatt aacaattatt ggtttagaaa caaacataag gtcagattat
    24781 gtttctataa aaagtggcgc acaaggcaca attttagaac cctcattcca gcaacaaata
    24841 aatatttcaa ctaaattaac agagaaagga atagatttaa tggctcaaaa agttatagaa
    24901 ggagatcttc taattagcgt tagtgaagca agtcttgcaa aagtttgggt tattaaagtt
    24961 ccaataaaag taagaattac ttttgggaat tctgtagaca acgtagactg tttagaaatt
    25021 aatcctcaag aaacaacaat aagagttgaa aattcaaacg atatagaata taatcttaaa
    25081 attaaaaata attgtacagt agatgatgag ccagtagata ttggcagaat ttatgcagaa
    25141 tcagactata agaacacaaa gaaattagga gattattttg tagtggtttc tggtaaagaa
    25201 caaaaattat cacttgatga aaaagtggaa gttgcaaata aaattgtagc agaagaaacc
    25261 gcaaccataa aattaatctt tagaccaaac aagtctgcaa aaagtggatt atcaaatcca
    25321 ataatcacat tctcatcaaa taggccaaat gtaaatggta tagatgtaat agagtcacag
    25381 catttaccaa gcataatatt aaacaattat gttagttgta tagaaacacc aagctctcca
    25441 attacaattc catattgtat gggttatggt tataatctat atgctggtgg aacaaacaca
    25501 atgaatttta atccattttt aatgggccaa tataattatc aatatgctgg ccaagcacca
    25561 caatcaatga atcaaagttg gttaactggt tcaacttatc caactagtat gatgggatat
    25621 caaagtgtat tgggaacagg agtcccatat acaaattata gagaaaatac ccctgcagga
    25681 tatcaaggtg ctcaaggctt ttcatcatct ggaatgtttg gttgtgagaa agcgcaaata
    25741 caaattagaa acaattgttc agaagaatta agtttacaat ttagtactct tggtggagta
    25801 ataattacag atcctgaaaa ttcagaaatg gcattaaaaa aaggcgcaag tggaatgtta
    25861 ggtgtacagg gctcacaagt tcttggacaa tacaagatag cagtaaatgc aaagccagac
    25921 gatgatgcta tagaagaata cacttatgta aaagacatag ttgtttcagt aaatagacca
    25981 gttagtattt tgcctgaaaa ttgtataagc gtagttccta cagaatttga tttctctgga
    26041 atagatacaa cagaaaagaa actaatggta atcaatacct gttatgacat gggctatgca
    26101 ttattaggtt taagtgtctt aaatgaagat caattaattg ttggagaggt tcaatatttc
    26161 tctattggag atgtaaatgc aaatcttaag ccaaagaaaa catatagata tgtagacggt
    26221 aaaaccacag aaataatgac catcaaaatt agaagaaatc ctgatattac agaaaaaaca
    26281 ttaagacttg atagcgaaga taataattct attgctaaga aactaaccgc actaagaaaa
    26341 gattattatg atgttggcaa gtccgtaaat ttaacagcat tacttggaat tacttattct
    26401 aatccaagat taggaaatga tgaaatggaa gcagagatta ctttaacaga taagcttcaa
    26461 tggttaagtt atatagatag ctttgcagac accagttctg ctcttgaagg tcaaacacca
    26521 ccaacagatt ccacagatac aacgaccggg gcacaagtac catcatttac agaatacttc
    26581 gcaggtagcg aggctctaaa tcctggcgag tgttatgatc caaaaggatt taagaaacaa
    26641 ggattcactg gagaagataa ttttaaaaaa tatggtttta acagattatt atttacatat
    26701 aatgatacag atataactca taatatatgt gattatggaa gagtctattg tgatcaagat
    26761 caattaagaa ttgccatatc taaaaaagca gatatgcaag gtgcaaataa aacctatcta
    26821 ttagatggaa catatttctt acttgaagga aataagattg cagcaaatcc ttatttcttt
    26881 gatgaagatg aaaatccatt agataaggcc gcaatagaaa gcatagtttc aagtcaaaca
    26941 tttaataatc gcgacccaaa ttcaatatta caggctttaa aagcagtatt aaatagagtg
    27001 cctgcagatt taagaaaatt tacaatactt gaagcaaaga ttggtccaat aactacaagc
    27061 gaaccaacac cagtaaatct ttcagcagat aacataaaag cgtatatgac catatttgga
    27121 gataatgact ttacaaagaa cgtaaagcaa gacggagacg tctatcaaat aactgttgat
    27181 tctttcttaa gaagtgaagg aaaattagaa gagaagatta atagcgcttc aacttcttta
    27241 caagagcaag caattattgc tgagttcttg aatcaatatc taaatacaat gcagttatat
    27301 tatggtgaca caataagtcc atttattatt aatgcaaaga catacggtgc attaaattca
    27361 ggaaatacga aatcaatatt tggtgctgtt cagttcatga gtggagcatg gaatttaact
    27421 ttagattcaa cgtctccagt aaaagaggtt gcaactccag gaacatacag cattgcattt
    27481 aagaattctt caactaataa acaattaact tatactgttc aaaaaataac ttctaattta
    27541 ttagattatt ataataataa tccagattat aagacaaaca ccttctttac aaatccaaca
    27601 aacgcttatt atttccatta tttcggggtg ccttttaaga ccgcaaatac cgggcaatca
    27661 aaagcaacag ttattattaa taactacaac aacggttggg ataaagtaca agatggtatt
    27721 atattaaatg ttccaaaaga caacgcagca atatctttta aagatataag accattacag
    27781 gttttatctt ctggggcata taagatagaa tatatagcaa atggcaaata ttcttcaaaa
    27841 gacacttcag aattaaaaaa tactttgttt attttccaaa aaataaatga aacaacaaaa
    27901 ggtgcagact atcaattaaa gacctcttca aacttagata tgaaatttaa tattgttccg
    27961 acaacactaa gtgctacaaa gaactttacg cacgtttatg gagtactaaa agacataact
    28021 agttcaagta attttacagc tgaagaaaca agttcagtaa agataatggt tgatggnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttc aagtaatttt acagctgaag
    28201 aaacaagttc agtaaagata atggttgatg gtatctcaag tgaaactatg tgtttcaatt
    28261 tcaataatgg ctttatgttg tggtacaatc ctcaaaaaac aacatttagt gttgatgcca
    28321 atatggcaac tactgaaaac gcaacagcgg gtacagggca aggttctata agtcctgcaa
    28381 caccaacgcc agccaccggc ccagcacaac tagcaataac aggcggaaac tttaatgtta
    28441 acacacctat agggtctgaa cctgcaaaag taaccggcgg taatttaact ttagatttag
    28501 aaaataaaaa attaagtgga actgttaaat ttgaatctgg aaataaaacc gcagaggttt
    28561 caatatctag tggtaaagca ataataagtg gcaacacagt gcaattaaat atctctggta
    28621 aagcgctggg atttgatgta actgcatctg gtaatttaaa tatttcaggt aatcaaggta
    28681 ttgatggaac ctatacaatt gtttctggac aaaaggcgtc attaacaggg ccagttttaa
    28741 cattaagtaa tttaaatttt acaaaaacag catagatata atataatata tgaaataata
    28801 ttcaaaaaat ttaaggtgta aaaaacatgg gattaaagaa attctattat tctatagaaa
    28861 ataaatggta tgattttgtt gaaaaaacag ggttgtataa agtaacagat aagattgaca
    28921 aatttatgcc ttctcttatt ttctttatac ttctaataat aatgataatt gcaggaatct
    28981 ttttattact gccaatgatt aaaggatcag gagatataga attaactttt aaagtaatgg
    29041 atgaatttga tgagccagtc ccattagcgc cagttttagt tactacttcc gatgacgctt
    29101 ataatcttaa aactaatgaa aacggcttaa cttcaaaaat tagattgcca ttcaatacta
    29161 aatattctgt agatattgat tattttggaa gtgattatga atcatatagc gaagaatttg
    29221 tcgcagaata tgacgacgaa atagatattt atttgtatag tatttctgaa gaaacaactt
    29281 ctacttattc ttttacacta gtcgatagtc aaacaagaat gcaaataaat aaaacaggaa
    29341 caattgaatt tatgtgtagt aattttggag tcttaccacc aaattcatta acatttagtt
    29401 ctggtaatat aacaattatt gcagatagtg attgtcaatt gaaagcaaaa tctataatga
    29461 ttgaaggtta tgttacaaga aacgacatat ccataaccca aaatataggc acaattgatt
    29521 ttgtttctag tgatccaatt gattttgaag cagtttatcc tgtttatgta acagttcttg
    29581 gagaagacgg aagtcctctt actggaatga gagcagaatt atatacaaat ggtattgcag
    29641 cgtatggaaa aaactgtaca acgacagctg gatcttgtac aatattaagt gtagatgctg
    29701 gcagctatgt tttgaaaata acagacacaa gagcagttcc agaatatgga ttagttgagg
    29761 aatcaataat tgtttcaggt attactccaa aaacaataaa tatgacaaga acaattgcag
    29821 gatatattca agtaaaagtt aagagaacaa ataatcaagc aatagaaaac gcagaagtaa
    29881 aattaaaata tggagacaat attatttcta gtgggcacac tacaaattca aatggtattg
    29941 ttaatgtacc agtttatgat ttaagtagaa catatagagt tgtggcttca aaggaaggat
    30001 atttggttgg ttcagcaagt aacattactg cttctgaaag cattccaaca aacccaacag
    30061 ttacaattac tttacaacaa ataactccac aaacaacagc gaccgtaaaa gtaactgtat
    30121 tagatagtgt aacagtaaaa ggttttagtc acgcgcaagt tgttctttat gattcaacta
    30181 ccggattttt aacagattat gattcgcaaa ttacaaattc tgatggcaac tgtacattta
    30241 gagtcgcacc aggcacatat tatgctaaag catttaaggg ttcttctgaa ggcgtaagtg
    30301 aagaatttga agtaattatc agtagctcaa ccgaagaagt aaatgtaatt attccaatag
    30361 aagtagttaa aacatcaata aatattactg ttgtggatca ttatggcgag ccagtaccaa
    30421 gagcaaacat tttattattt gataaatata attctagaag agcaatttgg ggtggggatt
    30481 tagcaaatat agacggtaca tttttaatat ctgatttagt ttcagattca gaagtttatg
    30541 ccgtaatttc agatattgca gataatcttg gtgctactca atcagagtac atatatctaa
    30601 aggcagacac aataaatgag ttaaaagtaa cgctatatcc aaaacaaaca agtcttcaaa
    30661 aaccagaaat taaattttta ggttttttta atgatgttgg agaaaaacta aatggttatt
    30721 taaaatcagg cgcagactac aaagcaagat ttttattatt aataccacaa gatagagacg
    30781 gcagagacga gtttgaagaa gttggggcgg ttgtaagaac tggctctcca agctcagttg
    30841 caagcccata tatggaaaac gatagtatct atataagatc aataaatcta ccaaacgcag
    30901 aagtcacaag atatactcag tatagctctg aaagtggtta tgacggtatt gatgatgttg
    30961 acactttaac tgaaggagac gcgaaatggg caaagatttt atttaatgaa atttatggtt
    31021 ccaattatgc aaatgcttat accattatgg cagacgtaaa agtaaaagac acagcagtct
    31081 atggcgaaga agttgcaatc aattatctag gttatggtta tacagaaaat aacagatatg
    31141 aaacagatcc aatagatgac acaccaaata gtaatgattt agaatatatg acacagaaaa
    31201 cagaaaactt taatattggc gaagaacaag catgttctga tgatttctgt tttaatttaa
    31261 atattattaa tcttgatgag gacttaagag aagatgttgt tgacaattat actgcttctc
    31321 caaatcaaca ttataaatta agatttggtt taatcaataa tgacgcagac acggtctata
    31381 atgtaaattc aagaatgcaa attaaaaacg aagacaaagg cctaaagttt agtagcatta
    31441 agataactca accaaatggt caattattaa caacaactgc aaatgagaat actagcgaat
    31501 atgatatttc aataactcaa tttaatccac acacaaagat attaggagag attgatttta
    31561 taccagttct aaaaggcgac agaatatttg atttaacttt tgtttctgat caaagagtaa
    31621 tttttacaaa gcaagtagca atacatgtgc tttctgataa aacctttata gttaagatta
    31681 cgccgaatgt aataccttca ggaaagaact tcaatcttca agttgaggtt aaagatgcaa
    31741 tcacaaatat cgaagttgat aagcaagtta atgttagagt aaaggacaga ttcaaaagag
    31801 atcttttggc aaacccagtt ccagtaggag ctttaggtat agcttcagta aataatattc
    31861 caggccaaga accaaacaac aaagtttatg tttatgttga ggcacaagag tatgagacaa
    31921 gtataagtga gataaagata actgacgaga tctttgagat cactccaaca agacttggag
    31981 tatccttaaa tattaatact aagaaaacag aaaatgcaaa gtttactatt gaaaatagaa
    32041 gcgaaagcga gttagtaatt gattctatta cctttatggg taacgacagt gaaatagatg
    32101 caatagatgt acaaagagta aataattcgt tattaggatt tgtaggcaca gagattcctg
    32161 gtgttgattc aagtgaaaag cctggcgcag ataattataa taataaacaa gagattactg
    32221 caagtttctt tacaagccct agagcaagca caatagattc aacaagaaac atcagttcta
    32281 aattagtaat taaactaaaa gataaatata ataaagagta tatctgggca acagaactac
    32341 cagtaacatt aactgttggt tatgaagggt taatggacga ttctcattgt atgactcttt
    32401 cagaagagtc ttgggagaca acagtagttg ataaatatgt agaaaagcaa tttactttta
    32461 agaacaactg cggagttaat tctaaaccag ttccattaaa tgggggtctt tctgctaaag
    32521 ttgagtatga aggcagtagt cctttagggg tatttacagt taatgttggt aacagattag
    32581 tggagttatc acatggctat tataagaaca tctatgactc tgtggattct gaaaagcaac
    32641 acaacgttgt tttaaaatat actcctgttg gtagatttac aggagacata aaaggaaaga
    32701 ttatttttag atcattaaat gacacaggct ctggtgctca agaattaata agcgaatata
    32761 agtttgtatt acatgtaata agcttaaaag actgttatgc ggtctcaaag aaagtcttaa
    32821 cagcaaaaga tactggcgag ccagacgcct ttgtaattga gaacaaaggt tgtggttcag
    32881 agataactta tcgtttatct tgtgatgact gtacgggctt agtattaaat ccaaaagaaa
    32941 acatagtagt tccagctacc ggatcaagtg aagagataaa agttatgtct cagggtgcaa
    33001 tgccaggaat ttatctatta aatgtatatt caaaggtaag tggagcaaga ggctcagaaa
    33061 gaaatgttgg aaaagtaaga gtagaagtaa ggccaacgac acaatgtatt gatcttgata
    33121 gatacgagta tgatttatac agatatgaat tttcagacac aggaaatcca acaaaagcaa
    33181 agagttacga tattggtaac attatcaata aatgttacgg tcaagacgta aacgttttag
    33241 gaaaaatgaa gaaagacgcc atctggaaga tggccctatg gaatggccta agagatggtc
    33301 tattcacctg gggtgtctct tctttaataa agaattttga agaagttact aaaaatataa
    33361 aatcaataac ctcatctgtt ttagctacag ttgctaaaga ttatacaaac tcaacacctg
    33421 ctcaaaaaac tgatgctcta atgaatgaat taaataaaat agacaaagaa ttagataaat
    33481 atttaaaata cttagataat ccaaaaatta agtccaaaga aaagacaaat attaagaatg
    33541 ctgtaactga ccttgaatcg ggtcttggta aattaaaagc aaaattccca gatcaagcag
    33601 gaattgcgcc tgcaaatgct ttggttgcag aaatagaaaa caaaattaca gaaattaaag
    33661 aaaagctaag ggataattct aaagagccag aatgttctac aacaaaacta tgtactgata
    33721 ctacaaaaga atgtaaagat ggtgtttgtg tagcaaagtc agctgagcca gaatgttcta
    33781 caacaaaact atgtactgat actacaaaag aatgtaaagn nnnnnnnnnn nnnnnnnnnn
    33841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33901 nnnnnnnnnn nnnnnnnnna tggtgtttgt gtagcaaaga cagtaactac ggtatatcca
    33961 acatattatc ttgcttcaag caataatgga acaagagcgt gtagtgaagc caatatccaa
    34021 ataggtcaaa aaggttgtgt gtgtgtgtat tccggtaata aaaaatgggt tgtaacagca
    34081 agatgtgcat caaataataa attatattta tataaggtag ctgcctctga agctgtttgt
    34141 actagtcaac agacaaatag ttcaacaatt tgcggtccga gaaaagatta aatttaatat
    34201 taataaggtg taaaaaatga aatcaaatct aaaatcaatt ttagtaacaa tgatagttgt
    34261 atcgttgttt tttaatattg gaacagtcta tgctgccgaa ggttctaact tcttaggaag
    34321 cgcaattagc caagttggcg gagtattaa
//
LOCUS       DAOOON010000010        24351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_2498_length_24351_cov_15.091332, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000010 DAOOON010000000
VERSION     DAOOON010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 24351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24351
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2498_length_24351_cov_15.091332"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="PLK55_02255"
     CDS             <1..1852
                     /locus_tag="PLK55_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35781.1"
                     /translation="INFYTNGNDLFLGGNFEFFSSNPNVISNLIINNSSRIIIGDNSE
                     IIELPNIVVNSEKQFHIMTINDYLTEKSSHDIIIESIFVNQNSTADIKIYSNPGKPTI
                     SDAIAGQNDLLTAGDSGDLKFQIIDLKNNGKTTIDLQTGIGGKGANGDGADNDDLIDN
                     DGPDMGDHAGNSGSINFNITNIENNNQLNINLKTGDGGTGGNSVIDLCTGAAIDEPIN
                     GGDGGNAGDITISKIEQIKNFGELTLEISGGNGGYAGSQRAERDWACDFGHNGSGGNG
                     GSISDLNIGILQNKLYNSVFNLKINSGTLGKVTLLPDSCADDPNNNGGKEDTSFGFPG
                     NIGNINIDFLENNSANDFEIISNFNFTPNDYDLAIAATNNGGIDGIDNGADMPSIALL
                     SPYLRLSDITINYLRNGSYLPKKIYAYTTIENYSFFNNDLRIDGCYISPTTVNYDIKD
                     DVIIVATNPESISVLNSNFDLTYRFCPHCEIMPLDDTQNRYTRDYSLYSNTNGIVNIG
                     DLNIHYSNPLDQSIYYTKRTDDKQLPVYTNKNPISGEWNPKINSYEYKIKPEDLNYYS
                     HTPEEDIDLLNQEDNLFCYGQEYIINGKINAGAIKPFNFPFVPLRELFTE"
     gene            1867..>2056
                     /locus_tag="PLK55_02260"
     CDS             1867..>2056
                     /locus_tag="PLK55_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35782.1"
                     /translation="MSEQEKLINEIKQTLEKNNPQTTSDKTEKPKKGFPKLIKKIKQI
                     FEKNNPQTTSDKTEKPKKG"
     assembly_gap    2057..2156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2157..8064
                     /locus_tag="PLK55_02265"
     CDS             <2157..8064
                     /locus_tag="PLK55_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35783.1"
                     /translation="NPQTTSDKTEKPKKGFPKIAFILIAAIVLIIILILLFSGGKKTL
                     GVYVVDEQNYSLGGVNVSLNNEITQSTDAFGYTEFKDLKFKDVEIKATKESYTTQTKQ
                     IKLNKKLQEETITLKVDTTSAEFRGITSERRREIIFKSYDVVVTDSLNVTFKCSNSEK
                     IPEPRATTVTNGRTNVLQPSNCGSLLVSVTSSNYELITDRNINENNIVELTRVNTNTG
                     MLEVTVKDISGQAVPYTNLRLNNIDDLQTSTNESDITFSTGITDIYGKYKFNVPQSNY
                     LLSIDKTGYISLPKLGPYNIINNNLTTASITLITASDLANLNCSNPIYAPFCKDGEID
                     CNSQLLTGLVTPNPNGTGCTIGRLTNLIVKLKDQNTSAPVTGNISVYRRDQNVLSLIT
                     TRRDVNQTTFVLNASNHYKILVSNTEDNGYLPPTPIDVNNLSADTNTTIEIPLEYLSS
                     LNSGSINVNVTKNGYKRANALVYLFYELDDEYVLYNPENPKSTNSNGDANFEFIRSNR
                     EYYAYAILRNESADGDSLPTQELDNNDILELEVSLSNQSKTLNLKITPSQDYNISFFE
                     VSGEQIPTESVVSTNVTGDTNKTFVFNCGEECSRIYLIIEKGDTLYQTDLISLFPGQV
                     AYKEIAFPTTLSEIQTTTSFLGLYDSTGTIEIDNLTFSGDNDLTKEYKIKFKTVFGSD
                     RTDVIKKAFIKAGRYLTENADFIYLTNTDLFVPEDTDIITGCRYHGDLESWDSNYFIT
                     NFESDYSFNNCTAGKYKWLEFDFSDINANIIEYSVNIKFKNEITTIDNYKIYYRSINS
                     NLETFSFSPNLSNWNDWSIRPEGYFYAPTQVEAMPFDNSNYSHVVKLYDQNGELTKFG
                     SDYILYIDNDYNYSLSFIQFSDTNRTGDIITDSQNTNENYVFTNSYFKDKTSTNPIIQ
                     TIDNNISFNISSKTTSIGYCFINYLIGKPIDFFSLTGQPALRTQLFKNNSSSLATALT
                     NVLSYSESAYVPVVTSKATDREIYVGSNDLNISLKNSTGNFENNVLVKYIISGQTIAT
                     DLGEITNNILNTEIFIPLEESGKTITFIFTILNSNLPNNELTLSKIIGTGIEIYDLNN
                     NLITNNNRLGYDILLTNISGEKKASESVKDYIIKNNINREVKLENVSISDSEAFLEEE
                     INQNLISVNELPKDLNQSNVISAGLFIDSNTNIQDINFNYNNLLTIKNTGDMPDITLY
                     KDTNSYARIKILNIQDFSKFTTTNVNGFYEDTNGSGIELIKDVISNISISYDLNLLFT
                     SPVNYDILDTQIVGEDISNYINFTQAHTNNLSQNRLLGNFNFTFVLKNINFTEIIEKD
                     ANINIKIGNQTENFSYEIPIKITLYPKNKSYNLASQGTHLAIACKEENDCNSTLVYTL
                     KNNTRSYDLNLTGIQLQDSNPLTYNVASEIQIPISITQNEIPIIIDGNYINLRTIPEL
                     HKIFTKQMNFSLNINNVPITETKPLTIIVTYIPEDPETELSNTGITSNFCMGVGGQAQ
                     GRDYVILGICDNGEQEMCSVGESALPKVLYQWRDHTQNWQTLCIDNQEEYDTNKIHCD
                     SVQALYSIFTLISSSDYDVDQNYFIYLMYDGIESEDLLEDFREHYNQFITHNISSIFS
                     ERYLDLYHDNEITITKRTPNDTPTKEVGKYKIVINNFDSLFTNNLNINLELVQNIPLN
                     KNNLFYYIPIDGDFGVTNNKAHRIGYGSTIVGDYDYSDLLVTEPSPTNIKLYEKQENC
                     TDDCSGVTNITASYPNNYLVNWQVEDVLKSNGNLLDLAVDNTTDFEKKNIVFNFTPSK
                     PVQVFARVNGINSKNFNYKLMINDQFSVSTANLIKWKEFSDSNYDNTIDDASVFGQHA
                     NYLRHELTKEKFKSKFGNNLAAKYLLESMIYLPVNIEYSNIKLTLLDLDNNLESKLYG
                     INSLEGTTELAITSKVSDIYSINQILNQVKDGNACISNTLSSSKIKWVTKKIEIPQGY
                     RYQIESNYNN"
     gene            8099..8635
                     /locus_tag="PLK55_02270"
     CDS             8099..8635
                     /locus_tag="PLK55_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35784.1"
                     /translation="MKKEKLTLFIWGFIIFFLGLIFALSFVHTTTSISRQNQEYLNYK
                     LYSARELNHFRNDFNQSAEELIFFRVSTYPKTTKSQIFAFKKEDFQKYTEKLSLQSVN
                     GDLFIVISKNSLEDKIKIKDQITDIEIPLIEYLKTGANYTGGNITTNFCPCFEFSSKA
                     IYHLYDCKDWGCRLSIIK"
     gene            8811..10190
                     /locus_tag="PLK55_02275"
     CDS             8811..10190
                     /locus_tag="PLK55_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35785.1"
                     /translation="MSITNCKNRGLLLIVVIVIAVFFVNNVFAINPEILGLKQSHLES
                     IICNYSSQTNNCVLEGGDYNITGEISPETSNINLVINQDTNFNVSCVSSNSVDNNCYI
                     MGDENRIDVVFSDFNSITIDSNVKININREHLIDTKLEFKNLDLIINGRLDITKDNNA
                     CLGEAYTNGYLYSYIPKIEVFFDKLEVGNNGILNTQIKKDYQGYDVGCGSYDSLTNLL
                     SSIEDTDWQAGEVTNLFVDKISFNDVINNGVINLNCQTKVDNTIYKYDNCNFYFNNLA
                     VNLPKKVINANNYGILFVSTCGPIYLPDNVDYNICKLNYIRPDTNVIDLNNANTGVAI
                     INKVNKFCVKGSISDQISEYLFTSDSNIESRPVIFGTINSAITSQPLDVSLASDNNSG
                     LYNTKNINQRISPISQTENIFFKLFLPNNYSTDTNLLDSYLFRFKLNSNSQVGYPEIG
                     LDYPFKIHK"
     gene            10205..12442
                     /locus_tag="PLK55_02280"
     CDS             10205..12442
                     /locus_tag="PLK55_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35786.1"
                     /translation="MLKIKAISKVLMVLIFIQLVIVSVYAENCFNPVTGISQDVSDDN
                     SSEYILKFNFTNESINQNFNFVRNSNNSLICNNKRFVLVIDDNESFKITGTNKEIKIS
                     GAIDEVRFDSNSQNPITLTFLVDNNESQLVGDNTPGVFNIDVNETRVLTNTKIVITEG
                     ERDFIESNGNASSPNRVGRGVRLNLRNLIVENNADLNISLQAITPNSGGTYGTYEYNN
                     NIFSTQHGMHGGTALFVAENILNNGKLNIDIFSAPGSKGRVIGDDDILNILGANGGDG
                     GRATFYVNNIYNYKDLNINVRSGNGADGSNGTDEGSLGGGLPMDGGVAGFGGQSDLYI
                     NDNLYNFINGVININVLSGSGGNGGNAGRNTFWSDNANGGNGGFGGFVFFNYGTGYNI
                     NIHNQGQLNYNVITGDSGFGGTKTKSGRKGSSGAGGDIFDFNVSRFVNTGDFNYYALG
                     GKVNINNDYIGTGRAGNIGSINIENLNNFSQNFNINIELNEKNIENISSITCACQENC
                     SIVGDPKIGNININYLRPGSFLPRSLKIIKQVPINDTTINISGCYVLSSGNSSISYAA
                     DNLILKLSNLADIQYDFDESENMTANNIFEKIVCPVCDSLELNNFALRTDIEYTIYTD
                     QGTENITDLNIYYVNPDGSLFKPPGYPSNKDYVIYSLKTGETITPKQNQFIGTNIKEY
                     KIAKEKLHYNSENFDLTNIPIKKGYDTQDVRLFCQAQRYLLRFRLQSQADIKEIYFTP
                     LFDIK"
     gene            12449..19024
                     /locus_tag="PLK55_02285"
     CDS             12449..19024
                     /locus_tag="PLK55_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOZ35787.1"
                     /translation="MNEKEEQVEKVYNMLKGKENRFSKDELERLIVFDGYDKDIAKRV
                     VKKLYDNKPKEEKPITTTPQEKEKILAEISKSLKGGSEEKPPLVAPKKDNKRIFSIIG
                     NFFKKIYFFFEDIYYSMIDGINKFIPISKLVDGIDKVFPSFILFLIFIIAIIWLIFFS
                     GVFGGLLGSPVLEITVTDSLDMPISGATAILSIGDLNKTSQTDVFGETIFQDFGKKKD
                     VKLIIQKESYNTQTKELKLKNGNNSVVVKLDIDTTTQLHLLAARTRNLVFKENDILLA
                     TRPILVNLRCANSGTIPEPNSTSINSGQLPVNIPTGCGDLRVDVSSEYYNSIQNTLVP
                     ENNVIMLTPIAQNYGTLEITVRDQNTQAAISDVFLQLYKPDDPTTQINESNLVVSSGT
                     TTVYGTYVFTNLTPGAYTVSANKSGYTSSLNRVGPYQISINNNTIGNIILSTGGKTLN
                     VVLVDINGNTEREIKGDITVYTKRTDTNVVTAIGTREDTNRATFSLPVNSSGVTYKLA
                     VQNTENSGYYAPDIMDLNYFDNNTTITIQLEYSSALNTGKIGVNVSRSTYKVVGASVY
                     IFRENHDDIPIAGPHITNNSGDCNFSLIRSGRKYFAYVIKSPEQGVSEVKKLDANDYM
                     ELNVELEDQATILNLKVMPTIDYNILFFKSNGDEVTEFVVTNTSQDSNKEYIFRDSAY
                     NMYAIISANGKATYQTSLITLIPGQRIYRSVSLSNTRTEAFSNIELLGIYDESGNIDK
                     NIINLATDYSKTFKLKFKLTTINQENRDKSYAYIRAGKRVALPSDYLRLENVIAFETE
                     NEYGCNFSGETTDWNQVHFLAYDDIDHSQANCSASPSGFKWVKIDFSDSDAEQIEFFV
                     DFKFQNGITNLSDYIIYYKSLTETEDEEYKLSPNLSNSWQECDMIPEGYFYSPYYNRA
                     LTFDNSDYAFLYDIYDYNSATNTIGQRLSLLGSKYILEIGNNYNYAQKMLYLKNSSAN
                     GSILATSYNTYNHLLYNAYKLKIRNQSSNTQTDINVTGINVVNYTIFDIDASLATQID
                     HNSVFTPIDFFINYSTGGSNQNSNSINQNNNSNQGNIAGMASATLNPQENGTNYDLNT
                     DSNLATNLLNISGLNPYSVPVLAYYPADANVIIEIITGDATGNNDIYAGDNNISFRVR
                     NISGSPISGVRISSTVYSPQAIIFGTTNSNGLLSNKNLSLPLSKLGSSINFSFLFPAN
                     FGFSNNTVVISKQIKSSYRLFLEDGTEISQGNAIQYNVGRYMNNGQLKFTKAEKNYYI
                     KGTNQVSGRLIAADPYPNPTQYLDFQDINNYIYTENNVPLYLSDLNKEIKTRISILNF
                     PNLDDNTNPLQDPKGQSQNNTIQNKLENAEYTSVTGGYHNIINAGNYNNLPIRLDLNA
                     PFNTNLFFNLNLETSFVGLPKPGVKESTEGAYDLIIELIKNRHSNINFNMAIANGSDN
                     RLLVDAIPVIDSSLNGYITVTTNDINIPSRSGNQLSIAIDLNNSKIITSDLRNKPLII
                     NLNYTINGVTFTEPISIKINVYDSNDAFVINQPTIKTMDCTANDCNTEIVVSATNKTR
                     TYDFNLSSINVLQPDNNLSITVSPPLPPIIINSQQTDNIKEITLKVSGNYNLLKGNTS
                     GSEYMRVIQEEITKTIKYNYKIIGSMEVGLTNQNFDINKNTNFKISIWLQNIQELLRE
                     NGLYGNICLGVGGININQPTGFYILGNCDTEGQNDCKNGADAKPKIIYDWSDQTDWKT
                     ECIENTSEDYNEQKTHCDSLQMLFSVFKLIENSTSNDISGNKFIYLMSDGVTKDLLSD
                     FVNNTEFMYSASTNWDNQDLLSDANIDANRFSISKTEIDSAGNEITTLKPGKYKITIE
                     NFIKGQPKPLHIKLELVREMPYTMRNLLYYLPIDGDLGIIGNNEQNKRSARDGYGSSL
                     TQSSKKGLPVEIGTGASQKEYYIYPIVNDENKSRAIINLQNDSNNFKNTLRSEGKLLD
                     LALTNIDSSDQSFNINMTYSPSYPIPLYAKVVNSDVSGLSYKLKKDEINDVAINFTNF
                     LVWQDYSNENRILSDGQYYSGVGVYYKSMIDGLKFKSVYGENNQIKLLKTMMYWPMNY
                     YAGDIPNINLVLSDNGSNCCGNNPESKIYSLVNLNGSSTNVGNIKNITRANSTLGTVD
                     LKDLLDYIDNNACIRSGLTVTTIKWIDENVGFTPEQANQIIENSGVSGINDNPTNEII
                     N"
     gene            19037..19420
                     /locus_tag="PLK55_02290"
     CDS             19037..19420
                     /locus_tag="PLK55_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35788.1"
                     /translation="MDLTILGFLNDMSFLLKLAFIVFIILFVKERITKNWLAITAIVI
                     VCIVLIFFYWPVFGSIYVVYILITIGIGSVLVDTFFVTMQEGAPKHKGEGPSPQQMLG
                     EQEQGYDMEQTFAARQQVMNRMGRR"
     gene            19420..19764
                     /locus_tag="PLK55_02295"
     CDS             19420..19764
                     /locus_tag="PLK55_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35789.1"
                     /translation="MNAKGFLNFIPFLKNASLIWKILIVILLVIFTVPLAVYAVPILK
                     YVIMIIIAFVVYGMVAPAFGNSLLTFIVLGIMLYFIFKYFYVATGAFLLYFFLVYGFM
                     SVLIWGTARFGR"
     gene            19803..19876
                     /locus_tag="PLK55_02300"
     tRNA            19803..19876
                     /locus_tag="PLK55_02300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19838..19840,aa:Arg,seq:gcg)
     gene            19898..20152
                     /locus_tag="PLK55_02305"
     CDS             19898..20152
                     /locus_tag="PLK55_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35790.1"
                     /translation="MHWKRTTFVLLILGILFFVIWNQIACQGICGVYQKNMSFIIFLV
                     ILVFTAIFLYYSLKLWAGAKKKYIEREEDKKEKTPEKGRK"
     gene            20242..20511
                     /locus_tag="PLK55_02310"
     CDS             20242..20511
                     /locus_tag="PLK55_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOZ35791.1"
                     /translation="MNKQTLIEEIGKKSDLSKSKIEETLNLTLDAVKAVVKKHKKLQL
                     VGFGTFAVKNRAARKGINPQTGKSIQIKASKSVGFKVSKEFKKSL"
     gene            20630..21202
                     /locus_tag="PLK55_02315"
     CDS             20630..21202
                     /locus_tag="PLK55_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35792.1"
                     /translation="MAKILKKIKEITNKKLADKRLEKQRQKKIEEQNRNAEENRKKLA
                     EFSRRAEEIQRVVKKVYENSSGLREISRTLRDPKITQRSCKNAFDVVSEGIRQKYGLR
                     FEFAEPGVVQALTNQPGNYATYNLRTKTFVMEKIPHPEDPRQINRYHMLKELRHEYGY
                     FLLLKEYGSRDNIPWVGNLAATHLLDQMHD"
     gene            21256..22548
                     /gene="hisS"
                     /locus_tag="PLK55_02320"
     CDS             21256..22548
                     /gene="hisS"
                     /locus_tag="PLK55_02320"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOZ35793.1"
                     /translation="MAKLILETARGTRDFPPNEKIIRDKVIYQIKEIFEIYGFSPLET
                     PTFEKLEILEAKFGAGENSDAISEIYRFKDQGNRDLGLRYEFTFALARFVGQNPQLKL
                     PFKRYQIGTVFRDGPIKAGRYREFYQCDIDIIGVSSVLADAESLAVVRDVFKKLKLDV
                     IIEVNDRKLLTEIIKYSGIEEKQATSVIISIDKLKKIGIKGVKEELLEKQIPKDKIEK
                     LLNLLTEIEKGTNAEKQKSLNKLLGETEGLKEIEELLGYLKDFSVEVEFSPSLARGLG
                     YYTGPIYEVFLKDQSIMGGSIAGGGRWDQMIGKYIGNENQNYSATGLSFGIEPIMDII
                     LKRDGTTRQTVTDLLVIPIKTQKEAIKVTQELRKRGLKVQVDLLERSVSKNIEYADKQ
                     GIPYVGFLGEDEIKQNKIKIRNLKSGKEELLDIDKVKL"
     gene            22603..23541
                     /locus_tag="PLK55_02325"
     CDS             22603..23541
                     /locus_tag="PLK55_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOZ35794.1"
                     /translation="MFVEERKTDSGKKYYLVKSYREGTKVKKFREYLGYNLSKTKIQV
                     LKEPAKERIQYKIANLRKIKDPLKNLLSKEEIERIRELQKEIQIFHLSKEQWIKFTEQ
                     FTYNTNAIEGSTLEEKEVREILEKNKWPIDKSKEDISEAQGVKEAIDYIRETKEHLSV
                     EIIREIHRIVFKNSKPFAGLFRPRGLEVVVSNGFGEIAHRGAPSTHVIELLEELTEWY
                     NKYKSKYPPILLAAVVHNQFENIHPFQDGNGRVGRILINNILLKHKIPPVDITLEKRI
                     EYYHTLQEYENNQNIRPTIELLLKEYKALRKKLNIK"
     gene            23603..23893
                     /locus_tag="PLK55_02330"
     CDS             23603..23893
                     /locus_tag="PLK55_02330"
                     /inference="COORDINATES: protein motif:HMM:NF022340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2683 family protein"
                     /protein_id="HOZ35795.1"
                     /translation="MCMKDTKGEFMINARIQLDPEANRILNMFKSKYDLKDKSEAINK
                     FVLVHSKVDEYDNLEVKDEYIYKMEKEVSKFLKSNPKFKSMTKKEFDDIFKK"
     gene            23874..24179
                     /locus_tag="PLK55_02335"
     CDS             23874..24179
                     /locus_tag="PLK55_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012193226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HOZ35796.1"
                     /translation="MTYSKSDILTFKWQFSEALISKITKLAKKDPVLHDILRKKMAEI
                     INQTPETIEHYKNLKYGLSDRMRVHIGKSHVLTFAYFRQENYIYFLDFAHHDEIYKK"
     gene            24240..>24351
                     /locus_tag="PLK55_02340"
     CDS             24240..>24351
                     /locus_tag="PLK55_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOZ35797.1"
                     /translation="MITTIELKQKYLDFFKSKDHKVIEQASLIPENDNTVL"
BASE COUNT         9858 a         3201 c         3832 g         7360 t
ORIGIN      
        1 aattaatttt tatacaaatg gcaatgattt atttttagga gggaactttg aatttttttc
       61 aagtaatcca aatgttataa gtaatttgat aattaataat tcctcgcgaa taattatagg
      121 cgataattca gaaataatag aattaccaaa tatagttgta aattcagaaa aacaatttca
      181 tataatgacc attaatgatt atttaacaga aaaaagttcg catgatatta taatagaatc
      241 tatttttgta aatcaaaatt caactgcaga cataaaaatt tatagtaatc caggaaaacc
      301 aactatctct gatgcaatag ctggccagaa tgatttgctt actgcaggag attcagggga
      361 tttaaaattt caaattattg atttaaaaaa taatggtaaa acaacaatcg atttacaaac
      421 aggtataggt gggaaaggtg caaacggaga tggtgccgat aatgatgatc ttatagataa
      481 tgatggtcca gatatgggag atcacgcagg aaattccgga agtattaatt ttaatataac
      541 aaatatagaa aataataatc agttaaatat taatttaaaa actggagatg gggggactgg
      601 aggaaatagc gttatagatc tttgtacagg ggcagccata gatgaaccaa ttaatggagg
      661 agatggagga aatgcaggtg atataacgat ctccaaaata gaacaaataa aaaattttgg
      721 ggaactaact ttagaaatat cagggggtaa tggaggatat gccggttcac aaagagcaga
      781 gcgagattgg gcttgtgatt ttgggcataa tggtagtggt ggtaatggag gcagtataag
      841 tgatttgaat ataggcatac tacaaaataa attatataat tctgtattta atttaaagat
      901 aaatagtgga acacttggta aagtaacttt gttaccagat tcttgtgcag acgatccaaa
      961 caataatgga ggaaaagaag atacctcttt tggttttcct ggaaatatag gtaacataaa
     1021 tattgatttt ttagaaaaca attcagcaaa tgattttgaa ataatatcta attttaattt
     1081 tacaccaaac gattatgatt tagcaatagc tgctacaaat aatggaggaa tagatggaat
     1141 agataacggt gcagatatgc catcaatcgc attactaagt ccatatttaa gattatcaga
     1201 cataaccata aattatttaa gaaatggatc ttatcttcca aaaaaaattt atgcttacac
     1261 gacaatagag aattatagtt tttttaataa tgatcttaga atagatgggt gttacataag
     1321 ccctacaaca gttaattatg acattaaaga cgacgtcata attgtggcaa caaacccaga
     1381 atcaatttca gtgttaaatt caaattttga tttaacatac agattctgcc cacactgcga
     1441 gattatgcct cttgatgata ctcaaaacag atatacaaga gattatagcc tttattctaa
     1501 tactaatgga atagttaata ttggagattt aaatattcat tattctaatc ccttagatca
     1561 gtcaatatat tatacaaaaa gaacagatga taaacaactc ccagtttata caaataaaaa
     1621 tccaatctct ggggaatgga atccaaaaat aaatagctat gaatataaaa taaaaccaga
     1681 agatcttaat tattatagcc acactcctga agaagatatt gatctattaa atcaagaaga
     1741 taatctcttc tgttatggtc aagaatatat aattaatgga aagattaatg ctggtgcgat
     1801 aaaacctttt aattttccat ttgtgccatt aagagaatta tttactgaat agaaaaaaga
     1861 ttaattatga gcgaacaaga aaaactaata aacgaaataa aacaaacatt agaaaagaat
     1921 aatcctcaga ccactagcga taaaacagaa aaaccaaaga aaggtttccc aaaattaata
     1981 aaaaaaataa aacaaatatt tgaaaagaat aatcctcaga ccactagcga taaaacagaa
     2041 aaaccaaaga aaggttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaat
     2161 cctcagacca ctagcgataa aacagaaaaa ccaaagaaag gtttcccaaa aattgcattt
     2221 attcttattg cagcaattgt tttgataata atcttaatat tattatttag tggtggcaaa
     2281 aaaacactag gagtatatgt agtagatgag caaaactatt cgcttggtgg cgtaaatgtg
     2341 tcattaaaca acgaaataac ccaaagcaca gatgcttttg gttacacaga gttcaaagac
     2401 ctaaaattta aagatgtaga aataaaagcc acaaaagaaa gctacactac tcaaacaaaa
     2461 caaataaaac taaataaaaa actacaagaa gaaactataa ctcttaaagt ggatacaaca
     2521 tctgcggaat ttagaggaat cacttctgag agacgcagag aaataatttt taaaagctac
     2581 gatgtagttg taacagacag tctaaatgtt acttttaaat gttctaattc tgaaaagatt
     2641 ccagaaccaa gagcaacaac agtaacaaat ggaagaacaa atgtattaca acctagtaat
     2701 tgtggttctt tattagtaag tgtaacgtct tcaaactatg aattaataac agatagaaat
     2761 attaacgaaa ataacatagt tgaactaact agagtaaata caaatactgg aatgttagaa
     2821 gttacagtta aagacattag cgggcaagca gtgccttata ctaatctaag actaaataat
     2881 atagatgatt tacaaacttc aacaaacgaa tcagacataa cgtttagcac aggaataaca
     2941 gatatttatg ggaagtataa gtttaatgtg ccacaatcaa attatctttt gtcaatagat
     3001 aaaacaggat atataagcct tccaaaacta ggaccatata atattattaa taataatcta
     3061 acaacagcaa gtattacttt aattactgct tctgatttag caaatcttaa ttgttcaaac
     3121 ccaatatatg cgccattttg taaagatgga gaaattgatt gtaatagcca attattaaca
     3181 ggtttagtta ctcctaatcc aaacggaacg ggctgtacaa tagggagact aactaattta
     3241 attgttaaac taaaggatca aaatacaagt gcgccagtta caggaaatat ttcagtttat
     3301 agaagagacc aaaacgtttt aagtttaatt acaacaagaa gagatgtaaa tcaaacaact
     3361 tttgttctta atgcaagtaa tcattacaaa atacttgtat ctaacacaga ggataatggt
     3421 tatttaccac caacaccaat agatgtcaat aatctttcag cagatacaaa cacaactata
     3481 gagattcctt tagaatatct ctcttcatta aatagcggaa gcataaatgt taatgtaaca
     3541 aaaaacggtt ataaaagagc aaatgcatta gtttatttgt tttatgagtt agatgacgaa
     3601 tatgtgttat acaatccaga aaatccaaaa agcacaaatt caaatggaga tgcaaatttt
     3661 gaatttataa gaagtaacag agaatattat gcttatgcaa tattaagaaa cgaaagtgca
     3721 gacggggact ctctaccaac acaagaactt gataataatg atattttaga attagaggtg
     3781 tctttaagta atcaatcaaa aactcttaat cttaaaataa ccccaagtca agactataat
     3841 atttcttttt ttgaagtttc aggagaacaa attccaacag aaagcgtagt ttctactaat
     3901 gttacaggag atacaaacaa aacctttgtt tttaattgtg gagaagagtg ttcaagaata
     3961 tatttgatta ttgaaaaagg agacacatta tatcaaacag acctgatatc gctatttcct
     4021 ggacaagttg catacaaaga aatagccttc cctacaacgt tatcagaaat tcaaacaacc
     4081 acttcctttt taggccttta tgattcaaca ggcacaatag agatagacaa tttaactttt
     4141 tcaggtgata atgatctaac aaaagaatac aaaattaaat ttaaaactgt atttggttca
     4201 gacagaacag atgtaataaa gaaagcattt attaaagcag ggagatatct aacagagaat
     4261 gcggatttta tttatttaac aaatactgac ctgtttgtac cagaagacac agatataatc
     4321 actggctgta gatatcatgg ggatttagaa tcttgggata gcaattattt tattacaaac
     4381 tttgaatcag attattcttt taacaactgt actgcaggaa aatataagtg gctagagttt
     4441 gattttagtg atattaatgc aaacattata gaatattcag taaatattaa attcaaaaac
     4501 gagattacca caattgacaa ttacaaaatc tattacagat caataaattc aaatttagaa
     4561 accttctcct tttctccaaa tctttcaaac tggaacgact ggtcaataag accagaagga
     4621 tatttctatg cgccaacaca agtagaagca atgccttttg ataattctaa ttattcgcac
     4681 gtagttaagt tatatgacca aaatggagaa ctaactaaat tcggttcaga ttatatactt
     4741 tatattgata acgactacaa ttatagttta agtttcatac agttttccga taccaataga
     4801 acaggagaca taattacaga tagtcaaaac acaaacgaaa actatgtttt tacaaatagt
     4861 tattttaaag acaaaacttc aacaaatcca attatacaaa caatagacaa taatatttct
     4921 tttaatattt cttcaaaaac cacaagcatc ggttattgct ttattaatta tttaataggc
     4981 aaaccaatag atttcttttc attaaccggt cagccagcac taagaacaca gttatttaaa
     5041 aacaatagct catctttagc aactgcttta acaaatgtgt tatcttattc agaaagcgca
     5101 tatgttccag ttgttacttc caaagcaaca gatagagaaa tctatgttgg ttctaatgat
     5161 cttaatataa gtttaaagaa cagtacaggc aactttgaga ataatgtctt agtgaaatat
     5221 ataatctctg gtcaaacaat tgctacagat ctaggagaaa ttactaataa catattaaat
     5281 acagaaatat ttattccttt agaagagtca ggaaaaacca taacttttat ttttacaatc
     5341 cttaattcta atcttccaaa caacgaatta accttatcaa aaattatagg aaccggcata
     5401 gaaatctatg atcttaataa taatttaata acaaacaata atagattagg atatgatatt
     5461 cttttaacaa atatttctgg ggagaaaaaa gcatcagaat cagttaaaga ctatattata
     5521 aagaataata taaatagaga agtaaaatta gaaaacgttt ctatttctga tagcgaggca
     5581 tttttagaag aagaaattaa tcaaaattta atatcagtta atgagttacc aaaagactta
     5641 aaccaaagta atgtgatttc tgcaggatta tttattgatt caaatacaaa tattcaagat
     5701 attaatttta attacaataa tttattaacc ataaaaaata caggagatat gccagacata
     5761 acactttaca aagacacaaa ctcttatgca agaattaaaa tattaaatat tcaagacttt
     5821 tctaagttta caacaactaa tgttaatggt ttttatgaag atacaaacgg ttcaggaata
     5881 gagctaataa aagatgttat tagtaatatt tcaataagtt atgatcttaa tctattattt
     5941 acaagccctg taaactatga tattttagac acacagattg ttggagaaga catctctaat
     6001 tatattaatt ttactcaagc acacacaaat aatttatctc aaaatagatt gcttggaaat
     6061 ttcaatttta catttgttct aaaaaatatt aattttacag aaattataga aaaagacgca
     6121 aatataaaca taaagattgg taatcaaaca gaaaatttta gttatgagat tcctataaaa
     6181 ataacactat atccaaaaaa taaaagttat aacttagcct ctcaaggtac acatctagct
     6241 attgcttgta aagaagaaaa cgattgtaat tctacgctag tctatactct taaaaacaac
     6301 acaagaagtt atgatttaaa tttaacagga atacaactac aagactctaa tccgcttact
     6361 tataatgtgg catcggaaat tcaaattcct ataagtataa ctcaaaatga gattccaata
     6421 ataattgatg gaaactatat caatctaaga acaatcccgg agttacataa aatctttact
     6481 aaacaaatga atttttctct aaatattaat aatgtgccaa tcacagaaac aaaaccacta
     6541 accataattg taacatatat tccagaagat ccagaaacag aattaagtaa cacaggaatt
     6601 acaagtaatt tctgtatggg cgtaggcgga caagcacaag gtagagatta tgtaatttta
     6661 ggtatttgtg ataacggaga gcaagagatg tgcagtgttg gagaatctgc tcttccaaaa
     6721 gtattatatc aatggagaga ccacacacag aattggcaaa ccctttgtat agataatcaa
     6781 gaagaatatg atacaaacaa aatacactgt gattctgtac aagcactgta ttcaatattt
     6841 acattaatta gttcttcaga ttatgatgta gaccagaatt attttattta tttaatgtat
     6901 gatggaatag aatcagaaga tttattagag gattttagag agcattacaa tcaattcatt
     6961 acgcacaata tttcatcaat attttcagaa agatatttag atttatatca cgataatgaa
     7021 attacaataa caaaaagaac gccgaacgat acgcctacaa aagaagttgg aaaatataag
     7081 atagtgatta ataatttcga ttctttattt acaaataatc ttaatatcaa cttagaatta
     7141 gttcagaata tcccattaaa taagaataat ctattctatt atataccaat agatggagac
     7201 tttggagtaa caaataataa ggcacataga attggttatg gttcaactat tgttggagat
     7261 tatgattatt cagatttatt agtaactgaa ccctctccaa caaacattaa attatatgag
     7321 aagcaagaaa attgtaccga tgattgttca ggagtaacaa acattactgc aagctatcca
     7381 aataactatc tagtaaattg gcaagttgaa gatgttttaa aatctaatgg caatttatta
     7441 gatttagcag tagacaacac aacagatttt gaaaagaaaa acatagtatt taatttcacg
     7501 ccttcaaaac cagtacaagt gtttgcaaga gtaaatggta taaatagtaa gaactttaac
     7561 tataaactaa tgattaatga tcaattctca gtaagcacag caaatctgat taagtggaaa
     7621 gagttttcag attccaatta cgataatact atagatgatg catcagtttt tgggcaacat
     7681 gcaaactatt taagacacga gcttacaaaa gagaaattca aaagcaaatt tggaaataat
     7741 ttagccgcga aatatttgtt agaatcaatg atttatttgc cagtaaatat agaatattct
     7801 aatattaaat taacattatt agatttagat aataatctag aatctaaact atatggaata
     7861 aatagtttag agggaactac agaattagcc ataacctcta aggtttcaga tatctattca
     7921 ataaatcaaa tattaaacca agtaaaagac ggaaatgctt gtatctcaaa taccttgtct
     7981 agttcaaaaa ttaaatgggt tacaaaaaag attgaaatcc cacaaggtta tagataccaa
     8041 atagaaagca attataataa ttaagattaa aaattaaatt aacaactgtg agaaggcaat
     8101 gaaaaaagaa aaacttacat tatttatctg gggatttatt attttctttt taggccttat
     8161 ttttgcttta tcttttgtgc atacaacaac cagcatatca agacaaaatc aagaatatct
     8221 aaattataag ctgtattcgg caagagaatt aaatcatttt agaaatgact ttaatcaatc
     8281 tgcagaagaa ttaatatttt ttagagtttc aacatatcca aaaacaacaa aaagccagat
     8341 ctttgctttt aaaaaagaag attttcaaaa atatactgaa aaattaagtt tgcagtcagt
     8401 aaatggagat ctttttattg tgatttcaaa aaacagttta gaagataaaa taaagattaa
     8461 agatcaaata accgatattg aaattccgct tatagaatat ttaaagactg gtgcaaatta
     8521 tactggtgga aatattacta ctaatttttg tccatgtttt gaatttagtt caaaagccat
     8581 ttatcatctt tatgactgta aagactgggg ctgtagatta agcataatta aatgatttta
     8641 tctaaaatcc tcctgtttta tccatgagaa gttatataaa tatatttaag taatatatta
     8701 tatagtaaat aggggaaaaa agagaatata tgaaattaac tctaaaggca attatagtta
     8761 tactcataat agaatttatt gttttattat tattttggtg attaataaaa atgtcaatta
     8821 caaattgcaa aaacagagga ttattattaa tagtagtaat agtaattgca gttttctttg
     8881 taaacaatgt ctttgcaata aatccagaaa tattaggatt aaaacaatcg catcttgaaa
     8941 gtataatctg caattacagt tctcaaacaa ataattgtgt tcttgagggc ggagactata
     9001 acataactgg agaaatatct cctgagactt cgaatataaa tttagtaatt aatcaagaca
     9061 ccaattttaa tgtttcttgt gtaagctcaa attcagtaga taataattgt tacataatgg
     9121 gagacgaaaa cagaatagac gttgtttttt ctgattttaa ttctattact atcgattcta
     9181 atgttaagat taatattaat agagagcatc taatagacac aaaacttgaa tttaaaaacc
     9241 ttgatttaat tattaatgga agattagata taaccaaaga taataatgct tgtttaggag
     9301 aggcatatac taacggatat ttatactcat atatccctaa aatagaggtt ttttttgata
     9361 aattagaggt aggtaataac ggaatattaa atacccaaat taaaaaagat tatcagggat
     9421 atgatgttgg ttgtggcagt tatgatagtt taactaatct gttatcatca atagaggata
     9481 ccgattggca agcgggagaa gtaactaatc tgtttgtaga taagattagt tttaatgatg
     9541 ttataaataa tggcgttatt aatttaaact gccaaacaaa agtggataat acaatatata
     9601 aatatgataa ttgtaatttt tattttaata atttggcagt taacttgcca aaaaaggtaa
     9661 ttaatgcaaa taattatggc atattatttg tctcgacttg tgggccaata tatttacctg
     9721 acaatgtaga ttataatatt tgtaaattaa attatataag accagataca aatgtgattg
     9781 atttaaataa tgcaaatact ggtgtagcaa taataaataa agtaaacaaa ttttgtgtta
     9841 aaggtagtat ctctgatcag atatctgaat atctttttac tagcgattca aacatcgaat
     9901 caagaccagt aatctttggg acaattaatt ctgcaattac ctctcaacca cttgatgttt
     9961 ctttagcctc tgataataat tcaggactat ataatacaaa aaatataaat caaagaattt
    10021 ctccaatatc ccaaacagaa aatattttct ttaagttgtt tttaccaaac aactattcaa
    10081 cagatacaaa tctattagat tcatatctat ttagatttaa attaaatagt aattcacaag
    10141 ttggctatcc agaaattgga ctagattatc catttaaaat acacaaataa tagaaagata
    10201 aaaaatgtta aaaataaaag caatatccaa agttttaatg gttttaatat ttatacaact
    10261 agtgattgtt tcagtttatg cagaaaactg ttttaatcca gtaactggaa ttagccaaga
    10321 tgtttctgac gataatagtt cagaatatat tttaaaattt aattttacta acgaaagtat
    10381 aaatcaaaac tttaattttg ttagaaattc aaataattct ttaatctgta ataataaaag
    10441 atttgtgtta gtaatagatg ataacgagag ctttaaaatt actggaacaa acaaagaaat
    10501 aaagataagc ggagcaatag acgaagtaag atttgattct aattctcaaa atccaataac
    10561 tttaactttc ttagtcgata ataatgaatc acaattagtt ggagataata ctcctggcgt
    10621 ttttaatatt gatgtaaatg aaacaagagt attaacaaac acaaaaatag taattaccga
    10681 aggagaacgc gatttcatag aaagtaatgg aaacgcaagt agcccaaata gagtgggtcg
    10741 tggggtaaga cttaatttaa gaaatttaat tgtagaaaat aacgcagact taaacatttc
    10801 tttgcaggcc ataactccaa attccggagg aacatacgga acatatgaat ataataataa
    10861 tatttttagt actcaacacg gaatgcacgg tggcacagca ttatttgtag cagaaaatat
    10921 attaaataat gggaaattaa atattgatat tttttcagca cccggttcaa aagggagagt
    10981 aattggagat gatgatattt taaatatatt aggcgcaaac ggcggagatg gcggtagagc
    11041 tacattttat gtaaataata tttataatta taaagattta aatataaatg tgagatccgg
    11101 taatggcgca gatgggtcaa atggaacaga tgagggtagt ttaggtggcg ggttgccaat
    11161 ggatggtggc gtggccggtt ttggcggcca gagtgattta tatattaatg ataatttata
    11221 taatttcata aatggagtaa tcaatattaa tgtactttca ggatctggcg gtaatggagg
    11281 aaatgctgga agaaatacat tctggtcaga taatgctaat ggcggtaatg gtggctttgg
    11341 cggatttgtt ttttttaatt atgggacggg atataatatt aatatacaca atcaaggaca
    11401 attaaattat aatgtgatta caggagatag cggatttggt ggaactaaaa ccaaatctgg
    11461 aagaaaagga tctagcggag ctggtggaga tatctttgat tttaatgttt ctagatttgt
    11521 taatactgga gattttaact attatgcttt aggcgggaaa gtaaatatta ataatgatta
    11581 cataggaaca ggaagagcag gaaatattgg ctcaataaat attgaaaact taaataattt
    11641 ttctcaaaac tttaacataa atattgaatt aaatgaaaaa aatatagaaa atataagtag
    11701 tattacttgc gcttgtcaag aaaattgttc aatagtagga gatcctaaaa ttggaaatat
    11761 caatattaat tatctaagac caggaagttt cttaccaaga tctttaaaaa taataaaaca
    11821 agtacctata aatgacacaa caataaatat atctggctgt tatgttctat catcagggaa
    11881 ttcaagtata tcatacgctg cagataattt aatattaaaa ttgtcaaatc ttgctgatat
    11941 acaatatgat tttgatgaat cagaaaatat gaccgcaaat aatatctttg aaaagatagt
    12001 ttgcccagtc tgtgacagcc ttgagcttaa taactttgca ttgagaacag acatagaata
    12061 tacaatttat actgatcaag gtactgaaaa tattacagac ttaaatattt attatgttaa
    12121 tccagacggt agtttgttta aaccaccagg atatccaagt aataaagatt atgttattta
    12181 tagtctaaag accggagaaa caataactcc aaaacaaaac caatttatag gaacaaatat
    12241 aaaagaatac aagatagcaa aagaaaaatt acattataat tcagaaaact ttgatttaac
    12301 aaatattcct ataaagaaag gatacgatac acaagatgta agactattct gccaagcaca
    12361 aagatattta ttaagattta gattacaaag tcaggcagat ataaaagaaa tatactttac
    12421 accattattt gatattaaat gatagattat gaatgaaaaa gaagaacaag tagagaaagt
    12481 ctacaatatg cttaaaggca aagaaaatcg attttcaaaa gatgaactag aaagattaat
    12541 agtctttgat ggctatgata aagatatcgc taaaagagta gttaaaaaat tatatgataa
    12601 taaaccaaaa gaagaaaagc caataacaac aacgccacaa gaaaaagaaa agattctagc
    12661 ggagatttct aaatctttaa agggcggttc agaagagaag ccaccacttg tggcgccaaa
    12721 aaaagacaac aaacgcatat tttcaataat aggaaatttc tttaagaaga tatatttctt
    12781 ttttgaagat atttattatt caatgattga tggcataaat aaatttatac cgataagtaa
    12841 actagtggat ggaatagaca aggtatttcc cagttttatt ttatttttaa tatttattat
    12901 tgctataatt tggttaatat ttttttctgg agtttttggc ggtctactgg gctctccagt
    12961 attagaaatt actgtaacag acagtttaga tatgccaatt tcgggagcta cagcaatatt
    13021 atcaattggg gaccttaata aaacctccca aacagatgtt tttggagaaa ctatctttca
    13081 agactttggt aagaaaaagg acgttaaatt aattatacaa aaagaatcat acaatacaca
    13141 aacaaaagaa ctaaaactta aaaatggaaa taattccgta gtagtaaaat tagatataga
    13201 tacaactaca caattacatc tactagcagc aagaaccaga aatttagttt ttaaagaaaa
    13261 tgatatacta ttggctacaa gacctatctt agtaaattta cgctgtgcaa attccggtac
    13321 aattcctgaa ccaaactcta caagcataaa ttctggccaa ttacctgtta atattccaac
    13381 cggttgcgga gatttaagag tagatgtttc ttctgaatat tataattcta ttcagaatac
    13441 attggttcca gaaaataatg taattatgtt aaccccaata gcacaaaact acggcacttt
    13501 agaaataaca gtaagagatc aaaatacaca agcagcaatt tcagatgtgt ttttacaatt
    13561 atataaaccg gacgacccaa caacacagat taatgaatca aatctagtgg tttcttcagg
    13621 aactacaaca gtttatggta cttatgtatt tactaattta acgccagggg catatacggt
    13681 ttctgcaaac aagagtgggt acacctcatc tttaaataga gttgggccat accaaatatc
    13741 gattaataat aacacgattg gaaacataat attatcaact ggtgggaaaa cattaaatgt
    13801 agttttagtt gatataaatg gaaatacgga aagagagata aaaggagata tcacagttta
    13861 tacaaaaaga acagatacta atgtagttac agcaataggc acaagagaag acacaaatag
    13921 agcaacattt tctttaccag taaactctag cggagtaact tataaattag cagtacaaaa
    13981 tactgaaaat tcaggttatt atgctccaga cataatggat ttaaattatt ttgataataa
    14041 tacaacaata acaattcaat tagaatattc ttcagcatta aatactggaa aaataggagt
    14101 taatgtttct agaagtacat acaaagtagt tggagcgagc gtatatattt ttagagaaaa
    14161 tcacgacgat atacctattg caggtccgca tatcacaaat aatagtggcg attgtaattt
    14221 ctcacttatt agatcgggaa ggaaatattt tgcttatgtt attaaaagtc cagaacaagg
    14281 tgtatctgag gttaaaaaat tagatgctaa tgattatatg gaactaaatg ttgaattaga
    14341 agatcaagca acgatattaa atcttaaagt gatgcccaca attgattata atattttatt
    14401 ttttaaatca aatggcgatg aggttactga atttgtagtt acaaacacct ctcaagattc
    14461 aaacaaagaa tatattttta gagattcggc atataatatg tatgcaataa ttagtgcaaa
    14521 cggtaaagca acatatcaaa ctagtttaat aacattaatt cctggacaaa gaatttatcg
    14581 ttcagtctct ctatcaaaca caagaaccga agcattttca aatattgaat tattaggcat
    14641 atatgatgaa agtggaaata ttgataaaaa tataattaat cttgcaacag attattcaaa
    14701 gacctttaaa ttaaaattta aattaacaac aataaatcaa gagaatagag ataaatctta
    14761 tgcttatatt agggcaggta aaagagtggc tttaccatca gattatttaa gattagaaaa
    14821 cgtaatagca tttgaaaccg aaaacgaata tggttgtaat ttctctggag aaacaacaga
    14881 ctggaatcaa gttcatttct tagcatatga cgatattgat cattctcaag caaattgttc
    14941 agcatcacct tctggcttta agtgggtaaa gattgacttt tctgattctg acgcagaaca
    15001 gatagaattc ttcgtagatt ttaaatttca aaatggcata actaatttat cagattatat
    15061 aatttattat aaaagcttaa ctgaaacaga agacgaagaa tataaattaa gtcctaattt
    15121 aagtaattct tggcaagaat gcgatatgat tcctgaagga tatttttata gtccatatta
    15181 caatagagca ctaacatttg ataattcaga ttatgcattc ttatatgata tttatgatta
    15241 caattcagca acaaacacaa ttggtcaaag attatctcta cttggtagca aatacatttt
    15301 agaaataggt aataactaca attatgctca aaaaatgcta tatctaaaga atagctcagc
    15361 aaatggatct atattggcta catcttataa tacttataat catttactat acaacgcata
    15421 taaattaaag ataagaaatc agtctagcaa cactcaaaca gatattaatg taacaggaat
    15481 taatgtagtg aattatacaa tattcgatat agatgcaagt cttgcaactc aaatagacca
    15541 caattctgta tttaccccaa tcgatttctt tattaattat tctactggcg gatctaatca
    15601 aaatagtaat tctattaatc aaaacaataa ttctaatcaa ggaaatattg ccggaatggc
    15661 aagtgccaca ttaaacccac aagaaaatgg cacaaattat gatttaaata ctgattctaa
    15721 tttagctaca aacttattaa atatctctgg attaaatcct tattctgttc cagttttagc
    15781 atattatcct gcagatgcca atgtaattat agaaattatt actggagatg caacaggaaa
    15841 taatgacata tatgcgggag ataataatat tagttttaga gttagaaata tttctggtag
    15901 tccaatatca ggagtaagaa tatcgtcaac agtttattct ccacaagcaa taatatttgg
    15961 tacaacaaac tctaatggcc tactatcaaa taaaaatttg tctttaccac tatcaaaatt
    16021 agggagcagc attaatttta gttttttgtt tcctgcaaat tttggatttt cgaataatac
    16081 tgtagtaata tctaaacaaa taaaatcaag ctatagatta ttcttagaag atggaactga
    16141 aatttctcaa ggtaacgcca tacaatacaa tgtcgggaga tatatgaata atgggcaatt
    16201 gaaatttaca aaagcagaaa aaaattatta tataaaaggg acaaaccaag tttccggtag
    16261 gcttattgca gcagatccat atcctaatcc aacgcaatat ttagatttcc aggatataaa
    16321 taattatatt tatacagaaa ataatgttcc cttatattta tcagatttaa ataaagaaat
    16381 aaaaacgaga atttcaatat tgaattttcc aaatttagat gacaacacaa atccactaca
    16441 agatccaaaa ggacaatccc aaaacaatac aatacaaaat aaattagaaa atgcagagta
    16501 tacttctgta actgggggct atcataacat aattaatgca ggaaattata ataatctgcc
    16561 aataagatta gatcttaatg ctccttttaa tactaatcta ttctttaatc taaatttaga
    16621 aacctcattt gtgggtttac caaaaccagg agttaaagaa tcaacagaag gcgcttatga
    16681 tttaattata gaactaataa aaaacagaca ttcaaatatt aacttcaata tggcaatagc
    16741 aaatggttca gataatagat tattggttga tgcaattcca gttattgatt catcattaaa
    16801 tggttatata actgtgacaa caaatgatat aaatattccg tcaagatcag gaaatcaatt
    16861 atctatagca attgatttaa ataatagtaa aataataact tctgatttaa gaaataagcc
    16921 attaattatt aatctaaact atacaataaa tggcgtaact tttacagagc caatttcaat
    16981 taagattaat gtttatgata gtaatgatgc gtttgttatt aatcaaccaa ccataaaaac
    17041 aatggattgt acagctaatg attgtaatac agagatagta gtttctgcaa caaacaaaac
    17101 tagaacttat gattttaatt taagtagtat taatgtattg caaccagata ataatctttc
    17161 aataactgtt tctccaccac tgccaccaat aattataaat tcacaacaga cagataatat
    17221 aaaagagata acattaaaag tatctggaaa ttataatcta ttaaaaggaa acactagtgg
    17281 ttcagaatat atgagagtaa tacaagaaga aataactaaa actataaaat ataattataa
    17341 gataattggt tcaatggaag ttggattaac aaaccaaaat tttgatataa ataaaaatac
    17401 aaattttaag atatctattt ggttacaaaa cattcaagaa ttattaagag aaaacggatt
    17461 atacggaaat atttgtctgg gtgttggcgg cataaatatt aatcaaccaa caggattcta
    17521 tatattaggt aattgtgata ccgaaggaca aaatgattgt aagaacggag cagacgctaa
    17581 accaaaaata atatatgatt ggtcagacca aacagattgg aaaacagaat gtatagaaaa
    17641 caccagcgag gattacaatg agcaaaagac acattgtgat tctttacaga tgttatttag
    17701 cgtatttaag ttaattgaaa atagtacgag caatgatata agtggcaata aattcattta
    17761 tctaatgagt gacggagtaa caaaagatct attatctgac tttgtaaata atactgaatt
    17821 tatgtattcc gcatctacaa attgggacaa tcaggattta ctatctgatg caaatattga
    17881 tgctaataga tttagtatat caaaaacaga aatagatagt gcaggtaatg aaataactac
    17941 tctcaaacca gggaaatata aaataacaat agaaaatttt ataaaaggtc aaccaaagcc
    18001 gctacacata aaactggaac tagtaagaga aatgccatat acaatgagaa atttattata
    18061 ctatttacca atagatggag atttaggaat tattggtaat aatgaacaaa ataaaagatc
    18121 agcaagagat ggttatggtt cttctttaac acaaagttca aaaaaaggtt tgcctgttga
    18181 gatagggacg ggtgccagcc aaaaagaata ttatatttat ccaatagtta atgatgaaaa
    18241 taaaagtaga gcaattataa atttacaaaa tgactcaaat aatttcaaaa atacattaag
    18301 atctgaaggt aaattattag acctggctct tacaaatata gattcgagtg atcaatcctt
    18361 taatattaat atgacctatt ctccaagcta tccaataccg ctttatgcga aagtagtaaa
    18421 tagtgatgtt tctggtttaa gttataaatt aaagaaagat gaaataaatg atgttgcaat
    18481 aaattttact aatttcctag tgtggcaaga ttatagtaat gaaaatagga ttttatcaga
    18541 tgggcaatat tatagtggtg taggagttta ttataaatcg atgattgatg gtttgaaatt
    18601 taaatcagtt tatggcgaaa ataatcagat taaattatta aaaacaatga tgtattggcc
    18661 aatgaattat tatgcggggg atattccaaa tataaattta gtattaagcg ataatggttc
    18721 aaattgttgt ggcaacaatc ctgaatcaaa aatatattct ttagtaaatt taaatggttc
    18781 ttcaactaat gtaggaaaca taaaaaacat aacaagagcg aattcaaccc ttgggacagt
    18841 agatttaaaa gatcttttag attatataga taataatgca tgtattagaa gtggacttac
    18901 agttacaaca ataaaatgga tagatgaaaa tgtgggcttt acaccagaac aagcaaacca
    18961 aataattgaa aattcagggg tgtctggtat taatgataat cccacaaacg aaataattaa
    19021 ttaaggcaga ataaaaatgg atttaacaat attaggtttt ttaaatgata tgtcgttttt
    19081 actaaagctg gcatttatag tatttataat tttatttgta aaagagagaa taactaaaaa
    19141 ctggcttgca attacagcaa ttgttattgt ttgtattgtc ttaatatttt tctactggcc
    19201 agtatttggg tcgatatatg ttgtatatat attaattaca ataggtattg gctcagttct
    19261 tgttgacact ttctttgtaa caatgcagga aggtgcccca aaacataaag gcgaagggcc
    19321 aagtcctcaa caaatgcttg gggagcagga acaaggatat gatatggaac aaacttttgc
    19381 tgctaggcaa caggtaatga ataggatggg tagaagataa tgaatgcaaa aggatttcta
    19441 aatttcattc cgtttttaaa aaacgcatca ctaatttgga agatattaat tgtaatttta
    19501 ttagttatat ttactgtgcc acttgcagtt tatgcagttc ctatacttaa atatgttata
    19561 atgataatta tcgcatttgt agtttacgga atggttgcgc cagcatttgg taatagccta
    19621 ttaaccttta tagttctagg gataatgcta tattttatat ttaaatattt ttatgttgct
    19681 accggggcat ttttattata tttcttttta gtttatggat ttatgtcagt acttatctgg
    19741 ggtactgcaa ggtttgggag ataatattat ttttaaacca tttagattca tattatttat
    19801 gtggatctat ggtgtagcct ggatagcatg taagcttgcg gagcttgcga cttgggttca
    19861 aatcccaata gatccatttt aaaatttgtg aaaaaatatg cactggaaaa gaacaacttt
    19921 tgtattgttg atacttggca tactattctt tgtaatatgg aaccaaatag cttgtcaagg
    19981 aatttgtgga gtatatcaaa agaatatgtc ttttattata tttcttgtaa ttctagtttt
    20041 tactgcaatc tttctatatt attctttaaa actgtgggcc ggagcaaaga aaaaatacat
    20101 agaaagagaa gaagataaaa aagaaaaaac tcctgaaaaa ggcagaaaat aagcgatttt
    20161 aaagcccaaa atgcagatat atttaaaaag agttagtatc aagataatat atcttttatt
    20221 atataaaggg ggtaaataaa aatgaataaa caaactttaa tagaagaaat aggaaagaaa
    20281 tcagaccttt caaaatcaaa gattgaggag acattaaacc ttacacttga tgcagtaaag
    20341 gcagttgtta agaaacacaa gaaattacaa cttgtaggtt ttggtacatt tgcagtaaag
    20401 aatagagcag caagaaaagg aattaatcca cagacaggta aatcaatcca gattaaagca
    20461 agtaaaagcg ttggattcaa agtaagtaaa gaattcaaaa agtctttata agtttagcaa
    20521 acacccagaa aaatctagtg atttttcttg ggtttctatt tttttaattt taaattaatt
    20581 ctatttttaa atattctgtt ttaagtattg tctataattg gagatagtta tggctaaaat
    20641 attaaagaaa ataaaagaaa tcacaaataa aaaattagca gataaaagat tagagaagca
    20701 aagacaaaag aaaatagaag aacaaaacag aaatgcagaa gaaaacagaa aaaaactagc
    20761 agaattttca agacgtgcag aagagataca aagagttgtt aagaaagttt atgaaaacag
    20821 ttctggttta agagagatat caagaacatt aagagaccca aaaattacac aaagaagttg
    20881 taagaacgca tttgatgttg tttctgaagg gataagacaa aaatatggtt taagatttga
    20941 atttgcggag cccggcgttg ttcaagcatt aacgaatcaa ccaggtaatt atgccacata
    21001 taacctaaga accaaaactt ttgttatgga aaagattcct catccagaag acccaaggca
    21061 aataaatcgg tatcatatgt taaaagaatt aagacacgaa tatggttatt ttctgttatt
    21121 aaaagaatat gggagtagag ataatatccc atgggttgga aatctcgcag caacacactt
    21181 gttagatcaa atgcacgatt aaagtttaga taggcatttt aaattgtttc gattcaaata
    21241 ttatatagaa aatacatggc taaactaata ttagaaacag caagaggaac tagagatttt
    21301 cctccaaatg aaaaaataat tagagacaag gttatttatc agattaaaga aatatttgaa
    21361 atatatggtt tttctccttt ggaaactcca acttttgaaa agctagagat acttgaagca
    21421 aagtttggag caggagaaaa ctcagatgcg atatcagaaa tatatagatt taaggaccaa
    21481 ggtaacagag accttggctt aagatatgaa tttacttttg cacttgcacg ttttgttgga
    21541 cagaaccccc agctaaaact tccatttaaa agataccaaa tcggcacagt ctttagagac
    21601 ggaccaataa aagcagggag atacagagag ttctatcaat gtgatattga cattataggc
    21661 gtgtcttcgg ttcttgcaga cgcagaaagc ttagctgttg ttagagatgt ttttaagaaa
    21721 ttaaaacttg atgtgataat tgaagttaat gatagaaaac ttttaactga aattatcaag
    21781 tattcaggaa ttgaagaaaa acaggcaaca tcagtaatca tttctataga caagctaaaa
    21841 aagattggta tcaaaggagt taaagaagaa cttcttgaaa agcaaatacc aaaagacaag
    21901 attgaaaagc ttctgaatct cttaactgaa atagagaaag gaacaaatgc agaaaaacaa
    21961 aaatccctaa ataaacttct tggtgaaaca gagggcttaa aagaaattga agaactttta
    22021 ggttatctta aggacttttc tgttgaggtt gagttctctc catcactagc aagagggctt
    22081 ggttattata ctggcccaat ctatgaagtg ttcttaaaag accaatcaat aatgggtggt
    22141 tcaattgctg gtggcggaag atgggaccag atgattggaa agtatatagg taatgaaaac
    22201 cagaattatt cagcaacagg actttccttt ggaatagagc caattatgga tattattctt
    22261 aaaagagacg gaacaacaag gcaaacagta acagacttgt tagttattcc aataaaaaca
    22321 caaaaagaag caataaaagt gactcaagag ctaaggaaaa gaggtctaaa ggtgcaagtt
    22381 gatcttttag agcggtcagt atctaagaat atagagtatg cagataagca aggtattcca
    22441 tacgtaggat tccttggcga agacgaaatc aagcaaaaca agataaagat aagaaaccta
    22501 aagtctggca aagaagaact gctggatatt gataaagtca aattataaaa tgtagccaaa
    22561 taaagctata tatttataaa ttgtagccac ctaattattt atatgtttgt agaagaaaga
    22621 aaaacagatt ctggtaaaaa atactacctt gtcaagtctt acagagaggg gacaaaagta
    22681 aagaagttta gagaatatct tggttacaat ctaagcaaaa ctaaaattca agtacttaaa
    22741 gaacccgcaa aagaaagaat acaatataag atagcaaact tgagaaaaat aaaagaccct
    22801 ttaaagaatc tattatctaa agaagaaata gagagaataa gagagctaca aaaagaaata
    22861 caaatttttc atttatcaaa agaacaatgg attaagttta cagaacaatt cacttataac
    22921 acaaatgcaa ttgaaggttc aactcttgag gaaaaagaag taagagaaat acttgaaaaa
    22981 aataaatggc caatagataa aagtaaagaa gatatctcag aagctcaagg agttaaagaa
    23041 gcaattgatt atataaggga aactaaagaa cacttgtcag ttgaaataat tagagaaata
    23101 catagaattg tttttaaaaa ctccaaaccc tttgcaggat tatttagacc aagaggttta
    23161 gaggttgttg taagtaatgg ttttggggaa attgctcata gaggcgctcc atcaactcac
    23221 gttattgagc ttttagagga actaactgaa tggtataata aatataaaag caaatatcct
    23281 ccaattcttt tagcagcagt tgttcacaat caatttgaaa acatccatcc ttttcaagat
    23341 ggaaatggaa gagttggaag aatactaata aacaacattc tgttaaagca caaaatacca
    23401 ccagttgaca ttactcttga aaaaagaatt gaatactacc acacacttca agaatatgaa
    23461 aataatcaaa acataagacc aacaatagaa ttactgctaa aagaatacaa agcactaaga
    23521 aaaaaactaa atatcaaata ggtgaccaca aaataagaaa atgtagtcaa acaacagatt
    23581 tataatcact ttgtatcact aaatgtgtat gaaagacaca aaaggtgagt ttatgataaa
    23641 tgcaagaata caattagatc cagaggcaaa tagaatactt aatatgttta agtcaaaata
    23701 cgatttaaaa gataaaagcg aagctatcaa caaatttgta ttagtacata gcaaagttga
    23761 tgaatacgat aatctggaag taaaagacga atatatctat aaaatggaaa aagaagtgtc
    23821 taaattttta aaaagcaatc caaaatttaa gagtatgaca aaaaaagaat tcgatgacat
    23881 attcaaaaag tgatattttg acttttaaat ggcaattctc agaagcccta atttctaaaa
    23941 taacgaaatt agcaaagaaa gatccagttc ttcacgatat attacgtaaa aagatggccg
    24001 aaataattaa ccaaactcca gaaacaatag aacattataa aaatctaaaa tatggattat
    24061 cggataggat gagagtacat atcggaaaga gccatgtact tacttttgct tattttagac
    24121 aagagaatta tatttacttc ttggattttg cgcatcacga tgaaatctac aaaaagtaac
    24181 cattgtcaag tattttaaat tatttccatt caccaaatat atattaagag agaaaaatta
    24241 tgataaccac aattgaactt aaacaaaaat acctagactt ttttaaaagt aaagaccaca
    24301 aagtaattga acaggcatcg ttgataccag aaaacgacaa taccgttctt t
//
LOCUS       DAOOON010000011        23233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_2682_length_23233_cov_18.357537, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000011 DAOOON010000000
VERSION     DAOOON010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 23233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23233
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2682_length_23233_cov_18.357537"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3486)
                     /locus_tag="PLK55_02345"
     CDS             complement(<1..3486)
                     /locus_tag="PLK55_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HOZ35798.1"
                     /translation="MSELNILANKETKEYYAKLTADIDAAVAIAKNARSQGKDLFKDI
                     ESVPAKGIAEKTELLVGPPGVAKVYNELWEKHKKRFPVMLEIFNMILDKELGNIEDPE
                     KRLDQALRTALIIETEGVVVSPLDGLPEIKLSKNQDDTNFIDIYYAGPIRAAGGNAQT
                     LPLLLGDLARKKLGIDRYKPSKKEIERVIEEAMLYQDEVVVRQQRVTEAEVKIMVENC
                     PIRMNSFADNEVEIVANRDVPGIDTNRVRGAMCLVLIDGLYVRALKIMKTSKRMGLDW
                     SWLEKLVKVKKTAEGFELKPSSKYLEGLAAGRPVFSYPFREGGFRLRYGKARNTGLMA
                     KAINPATMYVLDEFIAVGTHGRIERPGKSLQYFPCSTIDGPIVLLKNKDVILINNTED
                     AKKYVAQIDKILFVGDMLISVGDFRKAGHPLIKAGYVEEEWISEINYLYRNEKINKQT
                     LEICKKFNDKPNPYTAVALSLRFGFPLYPKYIFYYDRLNHEELKTLIRHVRDADKIFT
                     TDEEEQKSKTNLFENIKEIFEKTIDTIDVIGIGDILSKKDTELIGLKIEFNENIKSVL
                     EKIGLPHKYLESENKILIEKEQAYPFLKTVGAMNAINPLEEFKEEIEDENRKTIEILS
                     LISKLDIREKSGFWVGARMGRPEAAHERDMAGKPHVLFPIGRGFGNSRDLIKATKKRA
                     TGESTHNFGVNDVEIKAYMCQSCKKILFENYCFDCKINTQEIRECINCKTQTFDKICS
                     KCKGETKVKVEHKINIDRLIEQAANNLGVGVPESIKGVKGLISKEKNAEPLEKGILRA
                     KHDIYIFRDGTCRYEALNATISQVSAKELGLTLEKIKELGYKKDYLGNDLKDENQRVS
                     IFPQDIIVDEHCGEYFLRVAAFIDDELEKFYNLPKFYNSKVKEDLIGQLVIGLAPHTS
                     AGIVGRIIGFSKSKLAWGHPYYITAKRRNVDGDQDSMLLLMDGMLNFSNAYLSAGRGG
                     RMDAPIAFTTVLSPFEIDDESHEMETNTEYPFEFYKATKNFTEADKVPEIVNAGKLLG
                     TEQQYDCIKFTHDTACFDEGPKQSAYISIKTMNDKVQKQAKLQERIAAVDNKDSLERL
                     LHYHLFPDIIGNTRAFARQKLRCVKCNKKYRRMPLSGVCDCGGKLILTIAQGSIKKYL
                     EVAKNLVIDYELNPHL"
     gene            complement(3483..4961)
                     /locus_tag="PLK55_02350"
     CDS             complement(3483..4961)
                     /locus_tag="PLK55_02350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HOZ35799.1"
                     /translation="MDSNIICYAQNKDLIISEDCLRLLNNNNYKKILDRLFEEKKIFV
                     STEDIRKVINSFNETNQEITTDGKNYRILDSLDVTGKKLSQGSVEDFLALFLDKYNTL
                     SEIIKKRVDFSYQTIADAKREVKGKEVHVIGMVLDKRTTKNGNLLLTIDDPTGKVNIV
                     VMEDKENKSANKYEVNPKEILMDNVLGFKCSVLSKDIFIAREIMYADMPYQTKTQTLK
                     DDSHVVILGDTHVGSKLFREDEFTSFIEWLNAFNVSDEDKKIIEKIKYVIVAGDLVDG
                     IGIYPKQYDELAITDIFEQYRAFEEYILKIPEEIEVFLMPGNHDAVRVSDPQPSIPEK
                     FLPRLYSKKNIHIMGSPSWIELEGVMFLIYHGVSLHGIFGKIKDLKMDHPDLAQKELL
                     KRRDLMPQFGEKQTFIPIEKNFLTIKEIPDVFICGHIHHHAYSKYKNCHLISTSCWQS
                     ITSFQMEMGHTPTVSKAILFNLKNQKLCIKDFEKQENKIECK"
     gene            complement(4966..5244)
                     /locus_tag="PLK55_02355"
     CDS             complement(4966..5244)
                     /locus_tag="PLK55_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HOZ35800.1"
                     /translation="MPKKKTIDTSKMIEEIVSEIEFLAEDGSIPKNLKTLILGIEKRL
                     EKKCDDLEISSILYELEDITNNNNVPQFCRSAVWSLISKLENLKESIK"
     gene            5385..6758
                     /locus_tag="PLK55_02360"
     CDS             5385..6758
                     /locus_tag="PLK55_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOZ35801.1"
                     /translation="MKISIYKKRALAPIISAILLLVVGVILVTIVLTWGKNFTTKNLD
                     SATDFGALSASDASLFVFSKTFKEGVVMFTYSPPSYLMDEEITITHYRIVDIPEMTSV
                     QLTTPVTLSASTNIVPLECLYEYSKSSADITIQFITTENKYIEVKTRDPNIVCTSGGT
                     GTEEDPIIVCNAEDLNDIRTDLDANYALGKDIDLQCFSRQDINGWEPIGTDASPFTGS
                     LDGQNHKISNLYINRPTADYVGLFKSATVESTIIKNLGLEDVNITGNGQTGGIFGSLE
                     YGQITNSYVSGYISGGASVGGLIGSGFNPNISNSHSTGEIRSNESAGGIAGTTEGGAY
                     NNCYSLANVTRNSGVSETSFGGFIGLIEGTPTVNNSYSTGQIIYEGTTNPTNRGFSSD
                     AITGTSCYWDTETSGQLTSGSGATGKTTAEMKTQSTFTGWDFSSIWGMDSSTNSGYPY
                     LRSNPPD"
     gene            complement(6773..7705)
                     /locus_tag="PLK55_02365"
     CDS             complement(6773..7705)
                     /locus_tag="PLK55_02365"
                     /inference="COORDINATES: protein motif:HMM:NF037134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOZ35802.1"
                     /translation="MNFPKIVKSKELLDNVITKVRRIKDFQKNELAHKYISAYQIAIT
                     DRLKGINDKLPDYEIKNEYYQMMFKNICSKSTMEKYKNHIVSTITVVNKISEQYKRLL
                     LRMQSRRSSKFDKDKFVRIKKEYVGRISSVIKNLDSTFAKLQDLEKEFARIASPDFNL
                     RTIVLVGIPNAGKTTFLTKITEATPEINSYAFTTKALNIGFFKRRQDIYQVIDTPGLI
                     HTDFKEMNAIEKQAIAAIKAISDLIVFVYNPTLNKEQQTEMLKTLRENNPEKKIVVSS
                     TYTKDQYLEGQENIGMKELIETYPVEKKKKSRWS"
     gene            7830..8912
                     /locus_tag="PLK55_02370"
     CDS             7830..8912
                     /locus_tag="PLK55_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35803.1"
                     /translation="MKVITEKNYKKLMILPFLMAIICLILIFVSPKVERGIDLKGGNQ
                     LILRYDGAPVDQLKLESSLKEKYGFQELSIRETTGPTSTGVIIEYSNSPEIETAKEER
                     SKLDYDNTALVDLKTTSKSFLAPLLAKNYLSQKDLDEIDSFTSKEDLKVYINETIILA
                     NNNFNNSIVNFVKSELNLGDEARIQTREISATLGSDFIRSSIKVGIIAFVLLSFVIFL
                     FFREIIPSGLIIFAATFDILAGLAGMAILGLPLSLTTIPTLLMLVGYSVDTDILLSTK
                     LLKNRTDDVFAATNKSMKTGMTMTITTLVTVAVMALISYFMQMTVILEIASILLFGLI
                     GDIISTWFFNAPALITYVQRKNKNKN"
     gene            8930..10579
                     /locus_tag="PLK55_02375"
     CDS             8930..10579
                     /locus_tag="PLK55_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35804.1"
                     /translation="MKSLLKKPIVILWLLILALSVILLLTKGLQYGVDFSGGTSFSIV
                     LENQVAPEDMPRVVSVINSRLDWAGLKDAKVTSSGNQYITAQLAESDPDEIARLKNTL
                     LKQGKFESIIDGDVLFVGEDIRTIYKDPAKGYGIQTMDKSGASQWVLPFMLSPEASRR
                     FAEMTFHKCSPVGYENNSVVYDCEKTYFFIDRPTDAIFIIDNETYLEEKQVPVSPEVV
                     TSEMVSFDEIISQINTDYYLVSDNLSEEQVSKIQEDFSKYQKAIVSSNVPESIRKQLT
                     DIGYKVVIINKPENGYWIWEATGLKSIISLTEGVANMSAPSTTSASFEVFNNLTITGT
                     ASSKEEAKSRLDELLVILESGSLPIPIESISTESISPFLGKEFLQNSLWIGLFALLTV
                     ALVLFIRYKVFNLIFPILISDFSELIISLGFLSLIQFRIDLATIAGILAAIGTGVDHE
                     IIITDELLKGTKEEEASSNNLSLAAKVRNAFFIIFAAVATVFVTMLPIIFFNVGLSKL
                     VGFAITVMFGAVLGAFITRPAFAEIAKFVVAKHELKKSKKD"
     gene            10583..11032
                     /locus_tag="PLK55_02380"
     CDS             10583..11032
                     /locus_tag="PLK55_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35805.1"
                     /translation="MLEELVTIANQPFFGPLTSVITGIAVLSLIILFLLFLSFIALYI
                     YFSLAWYTIAKKLKYKNAWLSWIPAANIFLLPILAKQKWGWGFFLFVPIVNIVLAIIW
                     CWEIYKRRGYPGALSLFKAGYIIPPVIPLVFILEIVITGIVAFEKKY"
     gene            complement(11034..11456)
                     /locus_tag="PLK55_02385"
     CDS             complement(11034..11456)
                     /locus_tag="PLK55_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35806.1"
                     /translation="MNKKQKIKFILVFIVAYALLLLLGVFVFGSLLVGLEEKLIHLLL
                     GSSVNYGAFDFVVYCSGIVSVSAYLGVIVGFLAIKQKINHKVVLVGILLLFLINLLRI
                     MLVMLSEKIGWHNGVHVISWFLMLFMILWLIKISMEKK"
     gene            11521..11892
                     /locus_tag="PLK55_02390"
     CDS             11521..11892
                     /locus_tag="PLK55_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35807.1"
                     /translation="MKKKSNVFLFVVVGIILLAIINLIIIQSNSNKNTKETTYTNFPE
                     VITDIDDDSEISVYCIKSNNCCEIANDCEYIWFTGGCYVPEYVTRVQKEAQEKGVHIG
                     EAPEREGVTCSCESNKCVTHG"
     gene            complement(11895..12551)
                     /locus_tag="PLK55_02395"
     CDS             complement(11895..12551)
                     /locus_tag="PLK55_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOZ35808.1"
                     /translation="MNPIFYFLFFYALGIVLWITKKYDIFTISLIFGMTGLFLYFGKT
                     ALFIYLLMYFSVAEGLALILKNKGENRSYINLLGNCLVGTVLVVFGQIYSAASTICGA
                     FADTMSSEIGRLSKSKPRLITNLKRKVEPGTNGGITFLGLFAALMVSTITFVFFYFVF
                     KVNIKTSLIIASFGIIGSLIDSFIGAAIENKGYLDNSQTNFLTTFIVGALSIIALIYI
                     "
     gene            complement(12573..13142)
                     /locus_tag="PLK55_02400"
     CDS             complement(12573..13142)
                     /locus_tag="PLK55_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35809.1"
                     /translation="MADKKSTVDNWKKKKWYDVSADPTFDEKKIGETVTILPKNLIGR
                     NIKKGLNELTGNIRDSSFEVTFKVNKVTGAKADTVISKFEAKTGFLRRMIRRQKSKIE
                     PVFYVTTKDGSKLKIKVMAITGAKFTTPLRTQARKAIVDFFNEEVSAKTTKENWNDLI
                     FQHLSERCKAKLLKLGYINKVMIIKVTLV"
     gene            complement(13151..13549)
                     /gene="rps15p"
                     /locus_tag="PLK55_02405"
     CDS             complement(13151..13549)
                     /gene="rps15p"
                     /locus_tag="PLK55_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019680.2"
                     /note="primary rRNA binding protein; helps nucleate
                     assembly of 30S; binds directly to the 16S rRNA and an
                     intersubunit bridge to the 23S rRNA; autoregulates
                     translation through interactions with the mRNA leader
                     sequence; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOZ35810.1"
                     /translation="MSEEKKELTKKEIYEQIEDLTKKGKTTAEIGLILKQKYGVKSVL
                     QATGKKIGKVQAELDLKKDKLPDELLYLIKKSVKLIKHKENNKKDTSAKRGYQKTVSK
                     INRLRKYNIKKKRIDGAWRYSDDSAKLLVK"
     gene            complement(13679..14251)
                     /locus_tag="PLK55_02410"
     CDS             complement(13679..14251)
                     /locus_tag="PLK55_02410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HOZ35811.1"
                     /translation="MKEINKGAEAIIFLDGKKIIKKRVSKDYRDPVLDKRIIKTRNKQ
                     ESTLLKRAKVAGINTPTIFSLTEDTIVMEKLNNTNEHSKVLTEIGKEIAKLHDNGIIH
                     GDLNLINILTTKDKKIYFIDFGLGYLSNKIEDKATDLLVFKKTLKASKKTEQFWEKIL
                     AGYQKQTLNKQTTDKIAEIEKRARYCRKAK"
     gene            complement(14262..14762)
                     /locus_tag="PLK55_02415"
     CDS             complement(14262..14762)
                     /locus_tag="PLK55_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35812.1"
                     /translation="MSLAHTRKPISVIGHIVILIIFVVAVYLLITTIANYQVNKIAKI
                     YDKYMVTRSNLIPDETGNYIAELKALGTDKAQAIIDYVYLYQEAKNLDRAIRYAATES
                     GCITRTTIARIETQMQDRDKVLLGFNNTYSKKLDKIYWDTYIDILENKYDIESLKEKA
                     TALSFC"
     gene            complement(14765..17134)
                     /locus_tag="PLK55_02420"
     CDS             complement(14765..17134)
                     /locus_tag="PLK55_02420"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOZ35813.1"
                     /translation="MALDKWNKELEKEIIKKEMDNRVFEFRDNDKPVYSIDTPPPYIN
                     SPVHIGHASTYSIMDFISRYKRLKGFNVLFPLGLDRNGLPIEMAAEKKFKISILTTER
                     EAYIEYCKKILEETTTETSKSFSMLGISFNTYEETGKIGSVYKTDSEKYRTLTQETFI
                     ELYKKGLIYLDEKVTNYCPGCKTTLADSEIDYKVVDSKFYTILFTVKETGEKIPIATT
                     RPELVCSLGMVIFNPEDERWLKLNGKTAISPLFNREVPIKAHPMAKTDKGTGLVMMCS
                     AGDYSDIRFFMEQGIKPIISIDISGKMNKNAEFLEGLSVKEARLKMVELLKEKELVIE
                     EKIIQHKVPICERSKHEIEFISLKEYYLKQMDYIEKMKEIAEKVNFTNPATKKILLDW
                     LEVVSIDWPISRRRYYATEIPIWYCKKCGEAILGEKGKYHEPWKEKAPVESCPKCGSF
                     EIEGETRVFDTWFDSSISPLFVLDYGSEFYKKHPVCTLRPQGKEIVRTWLYYTLLKCY
                     LFTDKIIFDDVYIHQHILDDKGNKMAKSLGNAIDPQEILRDYGAEAFRLWVADEGNLN
                     EKDLKCSKDRISTEQKTINKLWNASKFISQFKRIDGINSEFSELDKTMLKEINNLIEL
                     CDNKYAGYDFHEPIVKLKYFLWTTLSSHYLELIKSRAYNQDGKFSKAEQNAAIDTLNY
                     CLERLLVLLYPVIPFTTHKILIEMFENDPNKESYPEIEKFESTLNLEDIENLNNTIWK
                     FKKDNNLSLKDEIKYLKINNKYKCMEKDIQKTHNVLNIQYLNNDSIEVK"
     gene            complement(17207..>17818)
                     /locus_tag="PLK55_02425"
     CDS             complement(17207..>17818)
                     /locus_tag="PLK55_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35814.1"
                     /translation="INNYETFIQELKLEKTEDKAYMDAYFKKKFIILENKKIIHPNIR
                     ELEKKVSELQETKDKLKGLKKFWEKEKDKAHNYYRDIRLKKYFKGKFWKHKIKQALNP
                     EYREDVINCKLTDEVIMDPEYGTLADTFLVDYDYRKKLSDTVNKSLIYKNNVIGDHNK
                     KRQQFKKENAITKIDHITEKLNATNLEILAYQTVIKYIKMVNK"
     assembly_gap    17819..17918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17935..18255
                     /locus_tag="PLK55_02430"
     CDS             17935..18255
                     /locus_tag="PLK55_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HOZ35815.1"
                     /translation="MFGGIDPRRMQSMMKQMGIENKEIEAIRVIIETNSKKIIIENPT
                     VTEVSMQGQKTYQIMGEIKEETGIPKEDISLVMEATKKSEKEAKEALEKTSGDIAKAI
                     ELLS"
     gene            complement(18259..19548)
                     /locus_tag="PLK55_02435"
     CDS             complement(18259..19548)
                     /locus_tag="PLK55_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35816.1"
                     /translation="MKMDKKAIFSIAIVLLFVGSMFAIMTTGTNRTKPGSDINSDLNV
                     NFTPVNYTTQFDANVLEVFPQIIVAGMPVDYDQTTIENKLLELQGLKSKNISYRQAED
                     GNINVVITLNITAEKKEEIVSKIKELEIIKEPIELYQYALLSVPADVNFTNDSNQTIE
                     YSFGATPIEGIVNIDTLKEDSIFVSCFATFTGQKLVSATGIEGYNNSSSPQMLVSSGD
                     FNVIDYGAQIYLEAQTMLIYPKDKTEIEAEIKIDYNNTEITYNFTQNLIYTLTEDKNK
                     EELKNALDNLKNEKLFSDYTLDEENLSLEVYLNEDINNTEYQNLKTQIKELSDATVIV
                     SEPKTNISIIFNYENIDVTKINNLLKSRGFSELKYKTIANVDTSNLIIEGKKYNYEQS
                     TTQAFVDYPEDLNKTTLTLQIQAYAQRDNILYLGLQK"
     gene            19625..20536
                     /locus_tag="PLK55_02440"
     CDS             19625..20536
                     /locus_tag="PLK55_02440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOZ35817.1"
                     /translation="MTAFKIGKVKIREKGVLAPMLEYTNLSFRRLCVENDCALPYTEM
                     VHINQILNCPLEKIQELKTEDEDYFVSLQLVGDFTDTKKTISAFKKIENLQFKIIDFN
                     LGCPSPKIEAGKSGAFLLDDIDKVSKTLKEIKSETDKSITAKVRLGTTKNNISVIAKS
                     LENAGIDAIAIHGRLKTQGYKTPSDYKEVEKIAKELSIPVIYNGDINLNNYKTFNDID
                     VFSGLMIGRTALNNPEIFSLISEKKPKTREENIKLFINLSKKYNLEIKYQKNNLLQLV
                     SGFSGSKRLREKLSIAKTQEDIEAVLY"
     gene            complement(20533..21258)
                     /locus_tag="PLK55_02445"
     CDS             complement(20533..21258)
                     /locus_tag="PLK55_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35818.1"
                     /translation="MELNKLSFPVKITLEEAILLFQKEFDKKGHKVKITKDKIHLNIT
                     PFWVCFYDIDCKTDGKYQHISAQTALNSLTNKVEDEFLKLLDVSTPKIIEDLDISFEK
                     VEIRIKKSLVKKEEAKETITKMLSCKFSVEKNNISLSGFEEIYMPIWRCDIENMEIYF
                     DGVTSKINNFNKIEKKQKNNREIFSEMLKDIKSPKKFFEYLFNLIVGVFKALFTTVKF
                     LIKNWYFTLIIILAIAIIIVVLF"
     gene            complement(21266..21607)
                     /locus_tag="PLK55_02450"
     CDS             complement(21266..21607)
                     /locus_tag="PLK55_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOZ35819.1"
                     /translation="MAFLNNVFSKKGDLDVDDYLNNIEIEEEPEEVDFWVKPISLQTN
                     AEVEEVIRELKERNIVLLDIENLSKRNAQRAKQFLGQIKMFVKDTGGDLAMISTSKIL
                     LTPSKVKIKKK"
     gene            21807..22976
                     /gene="thiI"
                     /locus_tag="PLK55_02455"
     CDS             21807..22976
                     /gene="thiI"
                     /locus_tag="PLK55_02455"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOZ35820.1"
                     /translation="MKTTHVIIHYSEIGIKKSNRFIFENALLRNINASCKEELSSSFK
                     RYGRLILVLKEDSNLEILKRNLENTPGVSSFSFSYSCQRDIKDIKKLAYDIAKSREKD
                     FSNFRITTTRSFKKFPLTSQEADAEIGADIFMLLKKPVKLKGSDLDIGVEITEKEVFV
                     YTNKDTFKSIGGLPVGSTGKLVCSLSGGIDSPVSGYMLNKRGCKVIYVHIQSNNLVTK
                     EVEDKIFKLCNVLKNFQGETKLIIVPFSDIQKQLLIAVPADYRMIIYRRFMFRIINKI
                     ADKENAQGIITGDSVGQVASQTLENLNCIYEAAKFPVFSPLIGLNKEEIIAIAQRIGT
                     YKTSIIPYPDCCSFMVAEHPKTKGSIADVKYIENNIKDIETLVDNAISNSKIYNF"
     gene            22986..>23233
                     /locus_tag="PLK55_02460"
     CDS             22986..>23233
                     /locus_tag="PLK55_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOZ35821.1"
                     /translation="MKDKRVVVGVSGGADSSMALVVLQQKGYTPIGVSLKLPVFSGAS
                     CKDNSCCTPQALAIAKDVCKKLGIEYHVVDERKNFEKEV"
BASE COUNT         6961 a         3818 c         3626 g         8728 t
ORIGIN      
        1 aagatgtgga tttagctcat agtctattac taggttcttt gctacttcaa gatatttttt
       61 tatagagccc tgtgcgattg taagaattaa tttccccccg cagtcgcata ctccagaaag
      121 tggcattctt ctatatttct tattacattt aacacatctt agtttttgtc ttgcaaatgc
      181 tctggtgtta ccaataatgt ctgggaaaag atgataatgt aaaagtcttt caagcgagtc
      241 tttgttatct actgctgcaa ttctttcttg aagtttagct tgcttttgta ctttgtcatt
      301 cattgtctta atagaaatat atgcgctttg ttttggccct tcgtcaaagc aagcagtatc
      361 atgtgtaaat tttatacaat catattgctg ctcagttcca agtagctttc cagcattaac
      421 aatttctggg accttgtctg cttctgtaaa gtttttagtt gctttataaa attcaaaagg
      481 atattctgtg ttagtctcca tctcgtgcga ctcatcgtca atctcaaatg gtgaaagaac
      541 agtagtaaaa gcaattggcg catccattct tccgcctctt cccgcactca aataggcatt
      601 agagaaatta agcatgccgt ccattaacaa aagcatagag tcctggtcgc catcaacatt
      661 tcttcttttt gcagtaatat agtaggggtg tccccaagcc aatttagatt tagaaaaacc
      721 aataattctt ccaacaattc ctgcagaagt gtgtggcgca agaccaataa caagttgtcc
      781 aatcaggtct tctttaactt tagagttata aaattttgga agattataaa atttctcaag
      841 ctcgtcatca ataaaagccg caactcttaa gaaatattct ccacaatgct catcaacaat
      901 tatgtcttgt gggaaaatag aaactctttg gttttcgtct tttagatcat tacctagata
      961 gtctttctta taacctagtt ctttaatttt ttcaagagtt aggcctagtt ctttagcaga
     1021 tacttgcgat attgtggcgt taagtgcttc gtatctgcag gttccgtctc taaatatata
     1081 gatatcatgc tttgctctca gtatcccttt ttcaagtggc tctgcgtttt tttctttact
     1141 aataagccct tttacgccct ttatactttc aggtactcca actccaagat tatttgctgc
     1201 ttgttcaata agcctatcaa tattaatttt atgttcgact ttaacttttg tttcgccttt
     1261 acatttgcta cagattttgt caaatgtttg cgttttacag tttatacatt ctcttatttc
     1321 ttgtgtattt attttacaat caaagcagta gttctcaaat agtatttttt tacagctttg
     1381 gcacatatat gcttttattt ctacatcatt tactccaaag ttatgtgtgc tttctccagt
     1441 tgctcttttc tttgttgctt ttataagatc tctagaatta ccaaagcctc tacctatagg
     1501 aaacaaaaca tgcggctttc cggccatgtc tctttcgtga gcagcttctg gtctacccat
     1561 tcttgcgcct acccaaaaac cggatttctc tctaatatca agcttagata tgaggcttag
     1621 tatctcgatt gtctttctat tctcgtcttc tatctcttct ttgaattctt caagaggatt
     1681 aatagcgttc attgccccca cagtttttag aaaaggatat gcttgttctt tttcaattag
     1741 tattttgttt tctgattcaa gatatttgtg cggcaaacca attttttcaa gtactgattt
     1801 aatattttca ttaaattcta tttttaatcc aattagctct gtgtctttct tgcttagaat
     1861 atctccaatt ccaataacat caatagtgtc gattgttttt tcaaatattt cttttatgtt
     1921 ttcaaaaaga tttgtcttac ttttctgttc ttcttcgtct gttgtaaata ttttgtctgc
     1981 gtctctaaca tgtcttataa gtgtttttag ttcttcgtgg ttaagtctat cataataaaa
     2041 tatatatttt ggatataatg gaaatccaaa tctcaagctt aatgccacag cagtatatgg
     2101 gtttggcttg tcattaaatt ttttacaaat ttctaatgtt tgtttattta ttttctcatt
     2161 tctataaaga taattaattt cagatatcca ttcttcttca acatatcctg ctttgattaa
     2221 cgggtgtcct gcttttctaa agtctccaac agagattagc atatctccaa caaacaaaat
     2281 cttatctatc tgcgcaacat atttttttgc gtcttctgtg ttattgatta gaataacgtc
     2341 tttattcttt aataaaacaa tcggtccatc aattgttgag caagggaaat attgtagaga
     2401 ttttcctggt ctttcaattc ttccgtgtgt tccaacagca ataaattcgt ctagaacata
     2461 cattgttgca ggattgattg cttttgccat tagcccggtg tttcgcgctt taccatatct
     2521 aagtctaaat ccgccttctc taaaaggata ggaaaatacc ggtctaccag cagctaatcc
     2581 ttcaagatat ttactagagg gctttagttc aaagccttca gcagtctttt taaccttaac
     2641 aagcttttca agccaagacc agtcaagtcc cattctttta ctagtcttca taatcttaag
     2701 tgctctaaca taaagcccat caattagaac aaggcacatt gcacctctta ctctgtttgt
     2761 gtcaatgcca gggacgtctc tattagcaac aatctcaact tcgttgtctg caaagctatt
     2821 cattctaata gggcagttct ctaccatgat tttaacttct gcttctgtta ctctttgctg
     2881 tcttaccaca acttcgtctt gataaagcat tgcttcttca attactcttt ctatttcttt
     2941 cttgcttggt ttatatctat ctattccaag cttctttctt gcaaggtctc caagtaatag
     3001 tggtaaggtt tgtgcgtttc ctccagcagc tctaataggc cctgcataat aaatatcaat
     3061 aaaatttgtg tcgtcttggt tttttgatag tttgatttct ggtaaaccat ctagaggaga
     3121 gacaacaaca ccttctgtct cgataatgag tgcagttctt agcgcttggt ctagtctttt
     3181 ttctgggtct tcaatattac caagttcttt atccagtatc atattaaata tttcaagcat
     3241 tactggaaat ctttttttat gtttttccca taattcgtta taaactttag caacgcctgg
     3301 cggtccaacc aaaagttctg tcttttctgc aattcctttt gcaggcacag actcaatgtc
     3361 tttaaaaaga tcttttcctt ggcttcttgc gttttttgca atagctaccg ctgcatcaat
     3421 gtctgcagtt aactttgcat aatattcttt tgtttcctta tttgcaagaa tatttagttc
     3481 actcatttac attcaatttt attttcttgt ttttcgaagt cttttatgca tagcttttga
     3541 ttcttcaaat taaacaatat tgcttttgag actgttggcg tgtgtcccat ctccatctga
     3601 aaagaggtta ttgattgcca gcaagaagta ctgataagat ggcaattttt gtattttgaa
     3661 taagcatgat gatgtatgtg cccgcagata aaaacgtctg gtatttcttt tattgttaaa
     3721 aagttcttct caattggaat aaatgtttgt ttttctccaa actgtggcat caagtctctt
     3781 cttttaagta gttctttttg tgcaaggtca ggatggtcca tctttaggtc ttttattttt
     3841 ccaaatattc catgtaatga tacgccgtgg tatataagaa acataacgcc ttcaagttcg
     3901 atccatgacg gtgagcccat aatatgtatg tttttcttag aataaagtcg cggtaaaaac
     3961 ttctcaggaa tacttggctg cgggtctgat actcttacag catcgtgatt tcctggcatt
     4021 aaaaatactt caatctcttc tggtatctta agtatatatt cttcaaacgc tctatactgt
     4081 tcaaatatat cagtgattgc aagttcatca tattgtttag ggtatattcc aatgccatca
     4141 actaggtctc cagcaacaat aacatatttt attttttcaa taatcttttt gtcttcatca
     4201 gagacattaa aagcatttag ccactcaata aaactagtaa actcgtcttc tctaaaaagc
     4261 ttactaccaa catgtgtgtc tcctaagata acaacatggc tgtcgtcttt tagtgtttgt
     4321 gtttttgttt gatacggcat gtctgcatac attatttctc tagcaataaa tatgtcttta
     4381 gatagcacag agcatttaaa tcctaagaca ttatccatta gtatttcttt aggattaact
     4441 tcgtatttat tggcgctttt gttttctttg tcttccataa ccacaatatt gacttttccc
     4501 gtcgggtcat caattgttaa aagtaggttt ccgttttttg ttgttcgttt gtcaagaacc
     4561 attccaataa catgcacttc ttttcctttg acttctcgtt ttgcgtcagc aattgtttga
     4621 tagctaaagt ctactctttt tttaataatt tcagaaagcg tattatattt atctaagaat
     4681 aatgctaaaa agtcctcaac agagccttgt gataatttct ttccagtaac atccaagcta
     4741 tcaagtattc tgtagttttt tccgtctgtt gttatttctt ggtttgtttc attaaatgag
     4801 tttattactt ttctaatatc ttcagttgac acaaatattt ttttttcttc gaaaagtcta
     4861 tcaagtattt tcttatagtt attattattt aaaagtctta agcaatcttc gctaattatc
     4921 agatccttat tctgtgcata acagatgata ttggaatcca tgttttcatt ttatactttc
     4981 ttttaggttt tctaatttac taataagcga ccaaactgcg cttctacaaa attgtggtac
     5041 gttgttgtta tttgttatgt cttctagttc gtaaagtata ctagatattt ctaggtcatc
     5101 gcatttcttc tctagtctct tctctatacc taatataaga gtcttaaggt ttttaggaat
     5161 agacccgtct tctgctagaa actcaatttc tgaaacaatc tcttcaatca ttttagaagt
     5221 gtcaatagtt ttcttttttg gcatttttat cacttccttt atttttaatt ataaaaatag
     5281 aaattaatat atattttagt agcaaaagct tttttaatag ctattggtta atttttttaa
     5341 ttaaaaccaa ggcattttaa tgttttggtt aatttatata tgtgatgaag atatctattt
     5401 acaagaaaag ggctcttgct cctattattt ctgcaatcct gttattggtt gtgggcgtta
     5461 tacttgtaac tatagtttta acttggggca aaaactttac cactaaaaat ttagattcag
     5521 caactgattt tggggcgtta tcagcgtctg atgctagtct ttttgttttt tctaaaacct
     5581 ttaaggaagg tgtagttatg tttacttatt ctccgccaag ttatttaatg gacgaagaaa
     5641 taacaataac tcactatcgg atagttgata ttccagaaat gacttctgtt caattaacaa
     5701 ctccagtaac tttatcagca agcacaaaca ttgttccttt agagtgttta tatgaatatt
     5761 caaagtccag tgcagacatt actattcagt ttattacaac agaaaacaaa tacatagaag
     5821 taaagacaag agatcctaat atagtttgta cttctggagg cacaggaaca gaagaggacc
     5881 caataattgt ttgtaatgct gaagatttaa acgacatcag aacagacctt gatgcaaact
     5941 atgctttagg gaaagacatt gatcttcaat gcttctcaag acaagatatc aacggctggg
     6001 aaccgattgg aacagacgcg tctccattta ctggttctct tgatggtcaa aatcacaaaa
     6061 taagtaattt atatattaat aggccaacag cagattatgt tggattattt aaatctgcaa
     6121 ctgtcgaatc tacaataatt aaaaatttag ggttagaaga tgtaaatata actggtaatg
     6181 gccaaaccgg gggaattttt ggttctttgg aatatgggca gattaccaat tcttatgttt
     6241 ctggttatat ttcaggaggc gccagtgttg gaggattaat tggttctggt tttaatccaa
     6301 atatttctaa ttcccattct actggagaaa tacgcagtaa tgaatcggca ggggggatag
     6361 caggaactac agaaggcggg gcttataata attgttattc tttagctaat gtaacgcgta
     6421 attctggtgt gtctgaaaca agctttggag ggtttattgg attaatagag gggactccaa
     6481 cagtaaataa tagttattca actggccaaa taatttatga aggaacaacc aatccaacaa
     6541 acagagggtt ttctagtgat gcaattacag gcacttcttg ttattgggac acagaaacgt
     6601 caggtcaatt aacaagtggt agtggagcaa caggaaaaac cacagcagag atgaaaactc
     6661 aaagcacatt tactggctgg gatttttcaa gcatctgggg aatggattca agtacaaata
     6721 gcggttatcc atatctaaga agtaatcctc cagattaaga tttaatttac aattagctcc
     6781 atctgctttt ctttttcttt tctacaggat aagtttctat aagttctttc atacctatat
     6841 tttcttggcc ttctagatac tggtcttttg tgtaggtact agatacgacg attttctttt
     6901 caggattgtt ttctcttaag gtttttaaca tctctgtctg ttgttctttg tttaatgtag
     6961 gattatatac aaaaactatt aggtctgata tggctttaat tgcagcaatt gcttgctttt
     7021 caattgcatt catttcttta aagtctgtat gtattaggcc aggcgtatca attacttgat
     7081 atatgtcttg tcttctttta aaaaaaccaa tgtttaatgc tttagtagta aaagcatagg
     7141 agtttatttc tggtgttgct tctgtaatct ttgtaagaaa agttgttttt cctgcatttg
     7201 gtattccaac aagaactata gttctaagat taaagtcagg acttgcaatc cttgcaaatt
     7261 ctttttctaa atcttgaagt tttgcaaaag tactatctag gtttttaata actgaagata
     7321 ttcttccaac atattctttt tttattctaa caaatttgtc tttgtcaaac ttactgcttc
     7381 ttctactttg cattcttaaa agtagtctct tatactgctc agaaattttg ttaacaacag
     7441 taatggtact aacaatatgg ttcttatatt tctccattgt gcttttagaa cagatatttt
     7501 taaacatcat ctggtaatat tcgtttttta tctcatagtc tggtagttta tcattaatgc
     7561 cttttagtct gtcagttatt gctatctggt aagcagaaat atacttatgt gcaagttcgt
     7621 ttttctgaaa gtcttttatt cttctaacct ttgtaataac attatcaagc agttcttttg
     7681 attttacgat ttttggaaag ttcatagcaa ttttctcttt atttctttaa ataaaaaagg
     7741 tatttaaagc atttggttaa taaaatatat aataatataa tattttatat atacttctta
     7801 ttttgaatag taaaacggtg taaagatcta tgaaagtgat tacggaaaag aactataaaa
     7861 aattaatgat acttccattt ctaatggcaa ttatttgttt aatactaatc tttgtctctc
     7921 caaaagtcga gagaggaata gacttaaagg gcggaaacca gttgattctt agatacgatg
     7981 gtgcgccagt tgatcagtta aagctagaaa gttctcttaa agagaaatac ggctttcaag
     8041 agctctcaat aagggagaca acaggcccaa caagcacagg ggtaatcata gaatactcta
     8101 atagcccaga gattgaaacc gcaaaagaag aaagatcaaa gcttgattat gataatactg
     8161 ctttagttga tttaaagaca acaagtaagt ctttcttagc accattacta gcaaaaaact
     8221 atctttctca aaaagaccta gacgagattg attcttttac aagtaaagag gatttaaaag
     8281 tatatattaa cgagacaata attcttgcaa acaacaattt taataattca attgttaatt
     8341 ttgtaaaatc agaattaaac ctaggagacg aagcaagaat tcagactaga gagatttcag
     8401 caactcttgg ttctgatttt attaggtctt caataaaggt cggcatcatt gcttttgtgt
     8461 tattatcttt tgttatattt ttattcttta gagagattat cccttcaggt ttaattatct
     8521 ttgctgcaac ctttgatatt ctagcaggtc ttgcaggtat ggcaatctta ggtctgcctt
     8581 taagtctaac aacaattcca acgctattaa tgcttgttgg ttattctgtt gacacagata
     8641 ttcttcttag cacaaagcta ttaaagaata gaacagatga tgtttttgca gcaactaaca
     8701 agtcaatgaa aacaggtatg accatgacta taacaacatt agtcactgtt gcagttatgg
     8761 ctcttatttc ttatttcatg caaatgacag taattttaga gattgcttca atattattat
     8821 ttggtttaat aggagatatt atttcaacat ggtttttcaa tgcaccagca ttaatcactt
     8881 atgttcaaag aaaaaataaa aacaaaaatt aaaaaaggtg tttttcaaaa tgaaaagttt
     8941 attaaaaaaa ccaattgtta ttctttggtt attgatttta gcgctttcag taattttatt
     9001 attaacaaaa gggctacaat acggtgttga tttctcagga ggcacttctt tttcaatagt
     9061 tcttgaaaat caagttgccc ctgaagatat gcctcgcgtt gtttctgtaa ttaatagtag
     9121 acttgactgg gctggcttaa aggatgcaaa ggttacttct tctggtaatc agtatatcac
     9181 agcacagctt gcagagtctg atcctgacga aatagcaaga ttgaaaaaca ctcttttaaa
     9241 gcaaggtaag tttgaatcaa taattgatgg tgatgtcttg tttgttggcg aagatatcag
     9301 aacaatctat aaggaccctg caaaaggtta cggcattcaa acaatggata agtctggtgc
     9361 aagccagtgg gttttaccat tcatgttgtc tccagaggct tcaagaagat ttgcagagat
     9421 gacttttcac aagtgttctc cagtaggcta cgagaataat tcagtagtat atgattgtga
     9481 gaagacttat ttttttattg acagaccaac agatgctata tttataattg ataatgaaac
     9541 atatcttgaa gaaaagcaag tgcctgtttc tccagaagta gtaacaagtg aaatggtttc
     9601 ttttgatgag attatctctc aaataaacac cgactattat cttgtaagcg ataatttatc
     9661 agaagagcaa gtatcaaaga ttcaagagga ttttagcaaa tatcaaaaag caattgtttc
     9721 ttctaatgtc ccagaaagca taagaaagca attaacagat attggctata aagttgtaat
     9781 aattaataag ccagagaacg gatactggat ctgggaagca acaggcctaa agtctataat
     9841 ttctttaact gaaggcgtag caaatatgtc tgctccttca acaacctcgg caagctttga
     9901 agtttttaat aatcttacaa taacaggcac tgctagttcc aaagaagaag caaaatcaag
     9961 acttgatgaa ctattagtaa ttctagaatc tggttctctt ccaataccaa tcgaaagcat
    10021 aagcacagag tctatctctc cattcttagg taaagagttc ttgcagaatt cgttatggat
    10081 tggtttgttt gcgttactta cagttgcttt agttttgttt ataagataca aagtatttaa
    10141 tttaatcttt ccaatattaa tttcggattt ttcagagtta ataatttctt taggattttt
    10201 gtctttaata caatttagaa ttgatctagc aacaattgct ggaattcttg cagcaatcgg
    10261 tactggtgtt gatcacgaaa taattatcac agacgaatta ctaaaaggca caaaagaaga
    10321 ggaagcatcc tcaaataatc taagcctagc agcaaaggta agaaatgctt tctttataat
    10381 ctttgcagca gttgcaacag tgtttgttac aatgctacca ataatcttct ttaatgtagg
    10441 tttaagtaag cttgttggtt ttgcaataac agtaatgttt ggtgcggttc ttggtgcatt
    10501 tatcacaaga ccagcatttg ctgagattgc taaattcgtt gttgcaaaac acgagttaaa
    10561 aaaaagtaag aaggactaaa gtatgcttga agaattagta acaattgcaa atcagccttt
    10621 ctttgggccg cttacttcag taattacggg aattgctgtt ctgtctttga ttattttatt
    10681 tttgcttttc ttgtctttta tagcactata tatttatttt tctttagcat ggtacaccat
    10741 agcaaagaaa ctaaaataca agaatgcgtg gctttcatgg attccagcag caaatatttt
    10801 cttgcttcca attctagcta agcaaaagtg gggttggggt ttctttttgt ttgtgccaat
    10861 agtcaatata gttcttgcaa ttatttggtg ctgggaaatc tacaagagaa gaggctatcc
    10921 aggggcatta agcctattta aggcaggcta tatcattccg ccagtaatcc ctttagtctt
    10981 tattttagaa attgttatta ctggtattgt tgcttttgaa aagaaatact aagttatttc
    11041 ttttccatac taatttttat aagccacaaa atcataaaaa gcattaaaaa ccaagatata
    11101 acgtgtacac cgttgtgcca gccaatcttt tctgatagca ttacaagcat tattcttaaa
    11161 aggtttatta aaaacaacaa gaggatacct actagtacca ctttatgatt gatcttttgt
    11221 tttatggcta aaaaccccac aatcaccccc aggtatgcag aaacagaaac tattcctgag
    11281 caatacacca caaagtcaaa agcaccatag ttaacagaag agccaagaag caaatgtata
    11341 agtttttctt caaggcccac aagtaggctt ccaaacacaa atactccaag caataatagc
    11401 aaagcatagg cgactataaa aaccaatata aattttattt tctgtttttt gttcattaac
    11461 aataatgatt agcatcttct tattaaatat atcgcaataa tattatttta gtgaattatt
    11521 atgaagaaaa agtcaaatgt ttttttgttt gtagtggttg gtataatttt attagcaata
    11581 attaatctta taatcattca aagtaattct aacaaaaata caaaagaaac aacctatact
    11641 aattttccag aagtcataac tgacatcgat gatgattcag aaatatcggt ttattgtata
    11701 aaatcaaaca attgttgcga aatagctaat gattgtgagt atatctggtt tactggtggc
    11761 tgttatgttc cagagtatgt aacaagagta caaaaagaag cacaagaaaa aggcgtgcat
    11821 atcggcgaag ccccagaaag agaaggcgta acttgttctt gtgaaagcaa caaatgtgtt
    11881 actcatggct aatttcagat atatatcaga gcaataatag aaagtgctcc aacaataaat
    11941 gttgttaaaa aattagtttg gctattgtca agataaccct tattctctat agcagctcca
    12001 ataaagctgt caattaagct tccaattatt ccaaagcttg caattattaa tgatgttttt
    12061 atattcactt taaaaacaaa ataaaagaac acaaaagtta ttgttgaaac cataagtgca
    12121 gcaaacaatc caagaaatgt tattcctccg tttgttcctg gttctacttt tcttttaagg
    12181 tttgttataa gtctaggttt agatttagat aatcttccaa tttctgaaga catagtgtct
    12241 gcaaatgccc cgcagattgt tgaagccgca gaataaattt gtccaaaaac aactaaaacc
    12301 gttccgacaa ggcaatttcc taaaagatta atatagcttc tgttctctcc cttgttttta
    12361 agtattagtg ccaggccttc tgctacacta aaatacatta aaagataaat aaacaatgcg
    12421 gttttaccaa aatacagaaa aagaccagtc attccaaata ttaaagaaat agtaaatata
    12481 tcgtatttct tagtaatcca aagaacaatt cctaaagcat aaaaaaaaag aaaataaaag
    12541 attgggttca tattttaatt gaactttctc ctttatacta aagttacttt aattatcata
    12601 actttgttta tataacctaa ttttaagagt ttagctttac atctctcaga aaggtgttga
    12661 aagataaggt cattccagtt ttcttttgta gtctttgctg atacttcttc attaaagaaa
    12721 tcaacaattg cctttcttgc ttgtgttcga agaggtgttg taaactttgc accagttatt
    12781 gccataacct ttattttaag tttacttccg tctttagttg taacatagaa aactggttca
    12841 atctttgact tttgtcttct aatcattctt cttaaaaaac cagtttttgc ttcaaacttt
    12901 gagatgacgg tgtctgcctt tgcaccagtt actttgttta ccttaaaagt tacttcaaag
    12961 cttgagtctc tgatgtttcc agttaactca tttaggcctt ttttgatatt tctaccaatt
    13021 aggttttttg gtaagattgt cactgtttct ccaatttttt tctcatcaaa agttgggtct
    13081 gcagagacat cataccattt cttctttttc caattatcta ctgtgctttt tttatctgcc
    13141 atcttttcac ctatttaaca agtagcttag cagaatcatc tgaatatctc caagccccat
    13201 ctattctttt ctttttaata ttatattttc ttagtctgtt tatcttagaa acagtctttt
    13261 gataccctct ttttgcgctt gtgtcttttt tgttattttc tttgtgtttg attagtttaa
    13321 cacttttctt aataagataa agtaattcat caggaagctt atctttttta agatcaagtt
    13381 ctgcttgtac tttcccgatc ttctttcctg ttgcttgtaa tacagactta actccgtatt
    13441 tctgttttaa aataagtcca atttcagcag tagtcttacc tttttttgta aggtcttcta
    13501 tttgctcata gatttctttt ttagttagtt cttttttttc ttcactcatt tatatcactt
    13561 tgatttttcc attaagtttg tgcctgtggc gattaattag gcgcgtttaa atagaaatat
    13621 agccgttaaa atcaggtcta taatatatat atctgctgcg atttatttat atatattatt
    13681 atttagcttt cctacagtat cttgctcttt tctctatttc tgcaatctta tcggttgttt
    13741 gcttgtttag ggtttgcttt tgatatcctg ctaagatctt ttcccagaat tgttcggtct
    13801 ttttgcttgc ctttaaggtc tttttaaaga caagaaggtc agttgccttg tcttctatct
    13861 tgtttgagag ataaccaagg ccaaagtcaa taaagtaaat tttcttgtcc tttgtggtca
    13921 agatattaat aagattaagg tctccatgga ttatgccgtt gtcatggagt ttagctattt
    13981 cttttccaat ctctgttaag acttttgaat gttcattagt gttgttcagt ttttccatga
    14041 caattgtatc ttctgttagg gaaaagattg tgggggtgtt gatgccagca acttttgctc
    14101 ttttcaataa tgtggattct tgcttgtttc tagttttaat tattcttttg tcaagcactg
    14161 ggtcacgata gtctttagaa acccttttct ttatgatttt cttaccatca agaaaaataa
    14221 ttgcttctgc gcccttattt atttctttca tgttttttca attaacagaa tgacaaagcc
    14281 gttgcctttt cttttaggct ttctatgtcg tatttgtttt ctaaaatatc aatatatgtg
    14341 tcccaatata ttttgtctag tttcttagaa taagtattat taaagccaag aagtactttg
    14401 tctctatctt gcatttgtgt ttcaattctt gctatcgttg ttcttgttat gcagccgctt
    14461 tctgttgcag catatctaat tgcccggtcc aggtttttag cttcttggta aagatacaca
    14521 tagtcaatga ttgcttgtgc cttgtctgtt cctagtgctt taagttcagc aatataattt
    14581 ccggtttcgt caggaataag gttgcttctt gtaaccatat atttatcata gatttttgct
    14641 atcttattta cttgatagtt tgctattgtt gttatcaaaa gataaactgc aactacaaat
    14701 ataattagaa taacaatatg cccaataaca cttattggct tgcgtgtgtg tgctaatgac
    14761 atttttattt cacttctatg ctatcattat ttagatactg aatatttaaa acattatgtg
    14821 ttttctgtat gtctttttcc atacacttat atttgttatt tatcttcaaa tatttgattt
    14881 cgtcctttaa tgaaaggtta ttgtcttttt taaatttcca gatggtgtta tttaggtttt
    14941 ctatatcttc taagtttaat gtgctttcaa atttttcaat ttctggataa ctttccttgt
    15001 ttggatcgtt ttcaaacatt tctattagta tcttatgcgt agtaaatggt attactggat
    15061 ataataatac taatagtctt tccaaacaat aattaagtgt atcaattgca gcgttttgtt
    15121 ctgctttact gaactttccg tcttggttgt aggctctgct ttttattagt tctaagtagt
    15181 gtgaggaaag tgttgtccat aagaagtatt taagtttaac gattggctcg tgaaagtcat
    15241 agcctgcata tttgttatcg caaagctcta ttagattatt tatttctttt aacattgttt
    15301 tatcaagttc agaaaactca gaatttattc catcgattct tttaaattga gaaataaact
    15361 tactagcatt ccatagcttg tttattgttt tttgctctgt tgagattcta tcctttgagc
    15421 atttcaagtc tttctcattt aggtttcctt cgtcagccac ccaaagtcta aaagcttctg
    15481 caccatagtc tcttaatatc tcttgtggat caatagcatt acctaaagac tttgccatct
    15541 tgtttccttt atcgtctaag atgtgctgat gaatataaac gtcgtcgaaa ataattttat
    15601 cagtaaaaag atagcacttc aaaagagtat aatatagcca tgttctaaca atttcttttc
    15661 cttgcggcct taaagtacag acagggtgtt tcttataaaa ctctgatcca taatccagaa
    15721 caaacaacgg agatatgctg gagtcaaacc aagtatcaaa aactctagtt tctccttcaa
    15781 tctcaaagga accacatttt gggcagcttt caactggcgc cttttctttc cagggctcat
    15841 ggtattttcc tttttctcct aagatagctt ctccgcattt cttacaatac catatcggaa
    15901 tttcagtagc ataatatctt cttctagata ttggccaatc aattgaaact acttctagcc
    15961 aatcaagtaa tatcttcttt gttgcaggat tagtaaaatt tactttctct gcaatttctt
    16021 tcatcttttc tatatagtcc atttgtttaa gataatattc ttttaatgaa ataaactcaa
    16081 tctcgtgttt tgatctctcg cagattggaa ccttgtgctg tataatcttt tcttctatta
    16141 ctagttcttt ttctttaagc agttctacca tttttagtct tgcttctttg acgcttaggc
    16201 cttctaaaaa ttctgcattt ttattcattt tcccagagat atcaatagat atgatgggct
    16261 taattccttg ttccataaaa aacctaatat ctgagtagtc tccagctgag cacatcatga
    16321 ctagaccagt tcctttgtct gtttttgcca ttgggtgtgc tttaattggt acctctctat
    16381 tgaacaatgg agaaattgca gtctttccat taagcttaag ccatctctcg tcttctggat
    16441 taaatataac catgccaaga gagcaaacaa gttctggtct agtggttgct attggtattt
    16501 tttcgcctgt ttctttaaca gtaaaaagga tggtgtaaaa cttagagtct acaactttat
    16561 agtcaatttc tgagtctgca agtgttgtct tacagccagg gcaatagttt gttactttct
    16621 catcaagata aataagtcct ttcttataaa gctcaataaa tgtttcttgt gttaaagttc
    16681 tgtatttttc tgagtctgtc ttatagactg agccaatctt tccggtttct tcataagtat
    16741 taaaagaaat tccaagcata gaaaagcttt tgcttgtttc agttgttgtt tcttctagta
    16801 tttttttgca atattcaatg taagcttctc tttctgttgt taatatcgag attttaaatt
    16861 tcttttctgc agccatttca attggtagtc catttctgtc aagtcctaaa gggaacaaga
    16921 cattaaagcc ttttagtctc ttgtatctag atataaaatc cattattgaa taagtagatg
    16981 catgcccgat atgcacgggg ctattgatgt atggtggtgg cgtgtctata ctatatactg
    17041 gtttatcatt atctctaaac tcaaaaacac gattgtccat ttctttttta ataatttctt
    17101 tttctagctc tttgttccat ttgtctagtg ccataagttg tttctcttta aaatataata
    17161 tataatatag gcaacttaat acttaaaaat agttgttttt tcgtgcttat ttattaacca
    17221 tcttaatata ttttataaca gtctgatagg ctagtatttc taggtttgtt gcatttagtt
    17281 tttctgttat atgatcaatc tttgtgattg cattttcttt cttaaactgt tgtcttttct
    17341 tgttatggtc tccgataaca ttgtttttat agattaatga tttattaaca gtatctgata
    17401 atttttttct ataatcatag tctactaaaa aagtatcagc aagtgttcca tattcaggat
    17461 ccataattac ttcgtctgtt agtttacagt ttataacgtc ttctctatat tccggattta
    17521 gcgcctgttt aatcttgtgt ttccaaaatt tgcctttaaa gtatttttta agtcttatgt
    17581 ctctataata attatgtgcc ttgtcttttt ctttttccca gaatttttta agccctttaa
    17641 gtttgtcttt tgtttcttgt agttctgaaa ctttcttttc tagttctcta atatttgggt
    17701 gtattatttt tttgttttca agaataataa atttcttttt aaaataagca tccatatatg
    17761 ccttatcttc tgttttttct aacttcaatt cttgaatgaa ggtttcgtag ttgtttatnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt aaatgtgata aattatgttt
    17941 ggtggaatag atcctagaag aatgcaaagt atgatgaagc agatgggtat tgaaaataaa
    18001 gagatagaag ctataagagt aatcatagaa acgaattcta aaaagataat tattgaaaac
    18061 ccaacagtaa cagaagtttc aatgcaaggc caaaagactt atcaaatcat gggcgagatt
    18121 aaagaagaaa caggcattcc aaaagaagac ataagcctgg taatggaagc tacaaaaaaa
    18181 tcagaaaaag aagcaaaaga ggctttagag aaaacctctg gagatattgc taaagccata
    18241 gagctattat cttaagttct atttttgtaa tccaagatat aatatattgt ctctttgcgc
    18301 atatgcttgt atctgtagtg ttaaagttgt tttattaaga tcttctggat aatcaacaaa
    18361 cgcttgtgtt gttgattgtt catagttgta tttcttaccc tcgattataa gattactagt
    18421 gtcaacatta gctattgtct tatattttaa ctctgaaaat ccgcgactct taagcaaatt
    18481 atttattttt gtcacatcta tattttcata attaaaaatg atgctaatgt ttgttttagg
    18541 ctcgctaaca attactgttg catcgcttag ttcttttatt tgtgttttaa ggttttggta
    18601 ttctgtgtta tttatatctt catttagata aacttctagg cttaaatttt cttcatctaa
    18661 agtgtaatct gaaaataact tttcattttt taagttatct aaagcatttt ttaattcttc
    18721 tttatttttg tcttctgtta gagtatatat taaattttgt gtaaaattat atgttatttc
    18781 tgtattatta tagtctattt ttatttctgc ttcaatttcg gttttgtctt taggatatat
    18841 taacattgtt tgcgcttcca gatagatctg tgcaccataa tcaataacat taaagtctcc
    18901 agagctaaca agcatctgtg gcgaactact attgttatag ccttcaatac ctgttgcgct
    18961 tactaatttt tggccagtaa atgttgcaaa gcaagaaaca aagatgctat cttcttttaa
    19021 tgtatcaata tttacaatac cttcaattgg tgttgctcca aatgaatact cgattgtttg
    19081 attagagtcg tttgtaaaat ttacgtctgc tggaacagaa agcagagcat attgatataa
    19141 ttctattggc tctttaataa tttctaattc ttttatttta gagactattt cttctttttt
    19201 ttctgcagta atattaagtg taataactac attgatattt ccatcttctg cttgtctata
    19261 agaaatattt ttgcttttaa ggccctgtaa ttctaatagc ttattttcaa ttgtggtttg
    19321 gtcataatca acaggcatgc ctgcaacaat tatttgtggg aatacttcta aaacgtttgc
    19381 atcaaattgc gtagtatagt taactggcgt aaaatttaca tttaaatctg aattaatatc
    19441 actgcctggt tttgtcctat tagttcctgt tgtcattatt gcaaacatag atcctacaaa
    19501 aagtaacact atggctatag agaaaattgc ttttttatcc attttcataa aaagttcacg
    19561 tatttttatt ctttttccta tataatatta tatataaaaa ctatttaaat aaatcatttt
    19621 tgttatgacc gcatttaaga ttggcaaagt caaaattaga gaaaaaggag tattagcccc
    19681 aatgttagaa tatactaatc tctcctttag gcgcctttgt gtagaaaatg attgcgcttt
    19741 accatataca gagatggtgc atattaatca gattcttaat tgtcctctag aaaaaattca
    19801 agagcttaaa acagaagacg aggattattt tgtttctctt caactggttg gagattttac
    19861 agacacaaaa aaaacaattt ctgcgtttaa gaaaatagaa aatctacaat ttaagattat
    19921 agactttaac cttggctgcc cttctccaaa gattgaagca ggcaaatctg gtgcttttct
    19981 tcttgatgac atagacaagg tttctaagac tcttaaagaa ataaaatcag aaacagataa
    20041 atctattact gctaaagtaa ggcttggtac tacaaaaaac aatatttccg taattgctaa
    20101 gtctttagag aatgctggaa ttgatgctat agcaattcat ggaagactaa agactcaagg
    20161 ttacaagacg ccctcagatt ataaagaggt tgaaaagatt gcaaaggaat tgtctattcc
    20221 tgtaatatac aatggcgaca tcaatctaaa taattacaag acgtttaatg acattgatgt
    20281 gttttctggg ttaatgatag gaagaaccgc tcttaacaat ccggagattt tttctttaat
    20341 ttctgagaaa aagccaaaga caagagaaga gaacattaag ctatttatta acctttcaaa
    20401 gaaatataat ctagaaatca aatatcaaaa gaataatctc ttacaattag tttctggctt
    20461 ttcgggttct aagaggctta gagaaaagct atctattgca aaaactcagg aagatattga
    20521 ggcggttcta tattagaata gtacaactat aataattgct atagccagta ttataattaa
    20581 agtaaaatac cagtttttta tcaaaaattt tactgttgta aataacgcct taaaaactcc
    20641 aacaatgaga ttaaatagat attcaaaaaa cttttttggg ctttttatgt cttttagcat
    20701 ttctgagaat atttcgcgat tgtttttttg ttttttttct atcttattaa aattatttat
    20761 ttttgaagtc acgccatcaa aatatatttc catattttca atatcgcatc tccagatagg
    20821 catatagatt tcttcaaaac cacttaaaga tatattgttt ttttcaacac taaacttgca
    20881 agatagcatc tttgttattg tttcttttgc ttcttctttt tttactaatg atttcttaat
    20941 tctaatttct accttttcaa aagaaatatc taaatcttct ataatctttg gcgtggaaac
    21001 atctaaaagc tttagaaatt cgtcctcaac tttgtttgtc aaagaattaa gtgctgtttg
    21061 tgcagagata tgctgatact taccatcagt cttacaatca atatcataga agcagaccca
    21121 aaaaggggtg atgttaaggt gaatcttatc ttttgtaatt tttaccttgt gacctttttt
    21181 gtcaaactct ttttggaata ataaaatagc ttcctcaaga gttattttga caggaaagga
    21241 taatttattt aattccatct ataatttact tcttctttat cttgacttta gatggtgtta
    21301 aaagtatctt tgatgtagag atcattgcta agtctccgcc tgtatcttta acaaacatct
    21361 ttatttgtcc aagaaactgc tttgctcttt gtgcgtttct cttgcttaag ttttctatgt
    21421 ctaaaagtac aatgtttctt tcttttaatt ctcttattac ttcttctact tcagcatttg
    21481 tttgtaagct gataggtttt acccaaaaat ctacttcttc tggttcctct tcaatctcta
    21541 tgttgtttaa gtaatcgtca acatctaaat ctcctttctt agaaaatacg ttgtttaaaa
    21601 atgccatttt tattcacctt ttttgtttat tccccttttt tttataaaaa tatagtaata
    21661 taatattgaa ccaaaatata taaaaacaaa ttgttatatg ggtagtctaa aattcttgta
    21721 tttttcgaaa cttcccttta taaaccttgt cttttaaaat tagtttcctt tttaatttgt
    21781 ttttgttctt aatatttata tcaactatga aaacaacgca cgtaataata cattattcag
    21841 agattggtat aaagaaatct aatcgcttta tttttgagaa tgcactttta agaaatatta
    21901 atgcatcttg taaagaggag ctttcctcgt cttttaaaag atatggcaga ttgattttag
    21961 ttttaaaaga ggattccaat ttagaaattt taaaaagaaa tttagaaaac acgccaggcg
    22021 tctctagctt ttctttttct tattcttgtc aaagggatat taaagatata aaaaaactag
    22081 catatgatat tgcaaaatct agagaaaagg atttttctaa ttttagaata acaacaacta
    22141 gatcttttaa gaagtttcca ttaacatctc aagaagctga tgcagagatt ggtgcagata
    22201 tctttatgct tcttaaaaaa ccagtaaaat taaaaggctc agatctcgac attggtgttg
    22261 agattacaga aaaagaggtt tttgtttaca ctaacaaaga tactttcaaa agcattggcg
    22321 gtctcccagt tggttcaaca ggaaagcttg tgtgttcttt gtcaggcggc atagatagtc
    22381 ctgtttctgg gtatatgtta aacaagcgcg gctgtaaagt tatctatgtg catattcaaa
    22441 gcaataactt ggtaacaaaa gaagtagaag ataagatctt taagctatgt aatgttctaa
    22501 agaactttca gggggaaacc aaacttataa tagtcccttt ctctgatatt caaaagcagt
    22561 tattgattgc agttcctgca gattatagaa tgataatcta cagaagattt atgtttagaa
    22621 taattaataa gattgcagac aaggaaaatg cgcaaggcat tatcacagga gactcggttg
    22681 ggcaagttgc ttctcagact cttgaaaact taaattgcat ttatgaagca gcaaagtttc
    22741 cggtcttttc accattaatc ggtcttaaca aagaagagat aatagcgatt gctcaaagga
    22801 ttgggaccta taaaacttca attatccctt atccagattg ctgttcattt atggttgcag
    22861 aacacccaaa gaccaaaggc agcattgcag atgtcaaata cattgaaaac aacatcaagg
    22921 atatcgagac gctggtagat aacgcaatct ccaattctaa aatttataat ttttaaaaga
    22981 ttattatgaa agacaaaaga gtagttgttg gtgtttctgg aggcgcggat tcttctatgg
    23041 ccttagtggt cttacaacag aaaggctata caccaattgg tgtttcatta aaacttccag
    23101 ttttttcagg cgcaagttgc aaagacaact cttgttgtac tcctcaggca cttgcgattg
    23161 caaaagatgt ttgtaaaaag ctcggaattg aatatcacgt ggttgatgaa agaaaaaact
    23221 ttgaaaaaga agt
//
LOCUS       DAOOON010000012        21071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_3071_length_21071_cov_17.625190, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000012 DAOOON010000000
VERSION     DAOOON010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 21071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21071
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3071_length_21071_cov_17.625190"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2036)
                     /locus_tag="PLK55_02465"
     CDS             complement(<1..>2036)
                     /locus_tag="PLK55_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35822.1"
                     /translation="FSANKKDILDLIYNYKTPGTMSNSNSKIEWLTVSGNNSLIKADK
                     FNVQKNGLNRVFCLSKVFAGKKLNIFVWQSKDSGNKINLGLGADNKKWTIPETDFYNT
                     GYLDKINYEFGYVTELQIPTNPFSSGTPPAEATPNEGVVNNGESSDSSEGDSAQPENQ
                     PEPVASQQIKLDFTVFSTIPDVKYSTTNTWYNARKINDPEAYLIDYRNDKKSISVNLS
                     SFNYTKPLYAIIATPDHSDKIYGYLANVKSTGDFKDYTLQSNFRISSNTLTTNPAAME
                     YTLGPDKTFKFSIDPKINDKFVLIFMQLSDAGSLINLAYTNLYHLDYNAQSIYQLCEI
                     NRNIINFYCKGYDCKVPGSCIDRWDFNASKLEGIQGIPVDAIPGAPTPPVTPTPPVET
                     PSVTPTPPVETSPPVNTAETLTFTGTCSITKQETLEKYIECLRRNIETVNLSRKYNFI
                     SDGKIIRKNGPFLGNFLIDEKYNTNNKCYEEAFNNVVFKIAKQEGLSEEETAQLWSRI
                     AAESGCNVKVGCGEVCGSTGVAQIHIPGWNDRGSYNSIKSELQKLKNWSSKYSTFSKY
                     QSSLLGIANDPETALEISIAINRHNTNLIINKSKTANRPFLADMYLTYDQDDSIDMEF
                     ATSYVYAGSARASHFTDGVKIGATRWQGLVAPALNKMGYYLTYKRMIYLCHNDA"
     assembly_gap    2037..2136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2137..3163)
                     /locus_tag="PLK55_02470"
     CDS             complement(<2137..3163)
                     /locus_tag="PLK55_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35823.1"
                     /translation="MNSKKIILSIFVIFAIFLMAANVFAENPGQKGEKPTGCDNDYGV
                     SDLKITGINLIKYDKSDQYGNCGEIIINTNIESGTGLTSMGIVVSVPLEYRKNYASYM
                     NGIYVVGSQEGKNAWQMCSGSSKASCVSPGDNLKTHKYYISNSEQCNSKSLDVYVFKP
                     KSAGTTEQDNVFVCQDVTEKGSTRTEIKREDGYSDLGMDNELIYKNGLSKSKWWLEYD
                     YKIKYLYNVDYAYGVPFSSSNPEGAQEASPNATAPATAITLSYSKLNPIYSGKIEGYR
                     DLSINDPIELVNDGLCTNSDYPYHLRISGGTTGLDVSETYIGFVGPNTFSANKKDILD
                     LIYNYKTP"
     gene            3357..4319
                     /locus_tag="PLK55_02475"
     CDS             3357..4319
                     /locus_tag="PLK55_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOZ35824.1"
                     /translation="MIEILKFSEHGIQRHERPTKDAWINVVNPDAYEIKYLAEICKIK
                     EVDYQEFKDDINSLQDMEEIPYMEKYRQEYFIITRTPHKLKSTDPESKYKSAVEYITI
                     PLGIMINKDYIITISFRENENVDILKTKKFKHEKAMVLLKLLLVSSKTYIKYLKEIDA
                     KIKNIEEKFESVQRNKEISDLLDLKKSLVYFNTSLKSDQILLEKIARNKDFVNSEDDK
                     DVIDDVIDENKQAIVVTAIYKNIIADATDLFNSVISNNLNKTLKLLTSITVVIAIPTL
                     VASIYGMNVTLPFDHSKYAFIIVMGIIAILSTLGIAILWKHDMF"
     gene            complement(4380..4868)
                     /locus_tag="PLK55_02480"
     CDS             complement(4380..4868)
                     /locus_tag="PLK55_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35825.1"
                     /translation="MKKLGELSLSQYIAKLVAISHWGAGAIFVIFGILIFISPTMLNS
                     LTGANPMFSEIGEINSFASKATGVVGVIMIIIAIYSFLVAYGFGTYKNYARIMLLIPL
                     YIFAGILALTGIIPSIILLFTMPVLGLISLTLIAFYGFIIFLSIYLFQKQKDMVALFK
                     GQ"
     gene            complement(4919..5689)
                     /locus_tag="PLK55_02485"
     CDS             complement(4919..5689)
                     /locus_tag="PLK55_02485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ35826.1"
                     /translation="MQSVKEFYNKYQYPKANQYTSNQKRTNKKIILGILETAGLKEKD
                     LKDKRILDAGCGTGEKSIFFAKAGAKEVVAIDFSKGQLEESRKKANKDKVKNLVFKEK
                     DILKYPLEELGKFDIIVCLGVLHHTENPKKSFSRIVGQLKRKGIIIIGLYHKYSRTKY
                     RLQRFFLRNLVSKDPDKIIAFVYKINKNQKAPKSTLYDRYAVPHESYHTLREVKGWFK
                     ENHISLIGHRNIRNEKLEITNIFEKKTIFFVSGKKKDI"
     gene            complement(5721..6674)
                     /locus_tag="PLK55_02490"
     CDS             complement(5721..6674)
                     /locus_tag="PLK55_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35827.1"
                     /translation="MSPKPKPKSTIGSRIGITAKKVGKKVAMGALIGTLAAAPSYYVG
                     KKVYSATKPSIVNQNIVLAKGNYLSEIKSVGQRITELNTNAKKEYSQKLLRSKNKEIK
                     RKLYFYDEKQNKIVALEMDVKKATEIEKKQNANFERLDKIIEKKVTELNKNAKPEEVY
                     FWNGDKISKLDLGVAKLRYIYDKLYSSEKAQLDNIYADIVKELKTSDKFSEEVALKMK
                     ELNDTARINYLSSLSVEEFAARFGNNPKVIKGLNEVIKSLEIYNEDSKNPRQSREVLN
                     FVQKESKLFGNIAGVGAGAVSGIVLLSLWSKIKAYSRKKKK"
     gene            6759..8246
                     /locus_tag="PLK55_02495"
     CDS             6759..8246
                     /locus_tag="PLK55_02495"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOZ35828.1"
                     /translation="MALLKEQEKLSFLLEKSIANLTKDKSKSYGLLFSGGVDSCLLAL
                     LLKKQKAKFTCFFAYVKDNTEAKDLAFAKKAAKELDLKLEIISADRKEISTILPKIID
                     IIDSTNPVSVGIAIPIYLSSRKAKTKKIDTIFSGLGSDELFAGYSRFTTYNQKTETKK
                     CLDNIQKDNLDRDEAIVKHFKLAIKCPYLDKAVIDYALTLPPRLFISSKQNKIIIRKI
                     AKNLGLSTGLAERKKLACQYGSNSDRTLKKLAKAKKFKTKSEYLSSFKKEKVGVLFSG
                     GKDSNLSLWLAQKDRKEISCLISLIPENVDSYMFQTPEVNFLKMQAFALDIPLLVTKT
                     KGQKEKELVDLRKAIVFAKAKFGLNGIVAGALYSNYQKDRIEKICKDLNLKLIAPLWH
                     MKEEDEIKLLLDNNFKFIICKIAAFGFSKEMLGKVIDYKDLEELKKLNRKYQTNIAGE
                     GGEYETLVLDTPIFKKRIIILESQKTMQNEFTGNLIIKKIKLEKK"
     gene            8282..9091
                     /locus_tag="PLK55_02500"
     CDS             8282..9091
                     /locus_tag="PLK55_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOZ35829.1"
                     /translation="MAAILAHMQFVRDAVKENPKLFSKSDLKYLLQGATYPDIYYITG
                     LRSITKKPNFSKFVHETMDEDYSFAKLLLERAKNQQEKLFALGFISHFVLDKHIHNYI
                     NSTELAHSVKHLVSEYYLDTKFKNQKIPIPRYPLKLIKTCFKEYYPMDYEKFKKRIKL
                     SINSIIFYDFANRFLIKKIVNARYRKGKYKTRLSLLNVPFKLARLSKYQKFGYDYRAL
                     LNPDISIKIEHTEKMYKLFLKAKEEFIDLVIDYELHISDYTSKQKRIIDFG"
     gene            complement(9362..9712)
                     /locus_tag="PLK55_02505"
     CDS             complement(9362..9712)
                     /locus_tag="PLK55_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35830.1"
                     /translation="MIGINTRTYPYRLDSKSKEPLELYIKLTNSQIKKMLSIDLVIDE
                     DLSFNSVKPINTDYKRIGEFNPGQVKEIRYSIYPAVGIYPGTKKIKIRVGEHNNDYNV
                     IDNKTETTVTITVI"
     gene            complement(9912..10997)
                     /locus_tag="PLK55_02510"
     CDS             complement(9912..10997)
                     /locus_tag="PLK55_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ35831.1"
                     /translation="MIIGITGFLASGKGAVSEILKQKGFIVYSCSDEIREECKKDNIE
                     ITRDNLQRVGNELRQKFGANILAKRLAERITLQGFDKNYVVESIRTEGEIEELKKLPN
                     FNLVFIDAEAMTRYQRAKERLKEKEHISSFEDFKNSEKKELNSKDPNSQNLESCKKLA
                     EFTINNNGSLKDLEKQVDDLLLKIQIKNRNKPSWDQYFLKIAEVVSLRGNCLSARFGS
                     VIVKDNVIKSTGYVGAPRGTTDCFEKGYCLRRKLGVPSGQQYELCVSVHAEQNAIINA
                     ARDGVSIFDGVLYLYGKKIYQGLEKPIDAFPCFICKKMIINAGIKRVVCSTADGKYKS
                     FEVNDWVEDWKKNDIIDDKEKYGTKYK"
     gene            complement(11010..11495)
                     /locus_tag="PLK55_02515"
     CDS             complement(11010..11495)
                     /locus_tag="PLK55_02515"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOZ35832.1"
                     /translation="MEKETIIIVAIAKNYAIGKDNKIPWHISEDFKRFKRLTTGHPVI
                     MGKNTYLSLPIKPLPGRTNIVLTFPDDSWKEEGITIKNSLNEAIDYAHTIDNQVYIIG
                     GASIYKQAMEQNLADKLEITQLDKEYEGDTFFPEINTNTWTIVAEEKKDGYSFITYLR
                     K"
     gene            complement(11517..12383)
                     /gene="thyA"
                     /locus_tag="PLK55_02520"
     CDS             complement(11517..12383)
                     /gene="thyA"
                     /locus_tag="PLK55_02520"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOZ35833.1"
                     /translation="MRAYLDIIRNIVEKGELKHNRTGIDTIAVAGCMFEHYMSEGFPL
                     LTTKKVPFRLIASELEFFIKGITDKNWLRNENNHIWDEWCSPDVVPYGNDEETKKKML
                     EERELGPIYGYQWRNFGKKYQSFDKEPTGEGVDQLKKLVDTLKTNPTDRRMIVSAWNP
                     LDIKHMALPPCHYGFQVTVINGKLNLLWNQRSVDSALGLPFNIASYGLLLHLLAKEAN
                     LKEGKLIGFLGDTHIYVNHVDGLKEQISRTPGVLPKIETENFTSIFDWKYTDTKIIDY
                     KPQPTIKFEIAV"
     gene            complement(12399..12830)
                     /locus_tag="PLK55_02525"
     CDS             complement(12399..12830)
                     /locus_tag="PLK55_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35834.1"
                     /translation="MAKIIDFQKAKAEVNKNKSKTLTVYIKEQERMIDFIGKSILDTL
                     SEVYVSTTIDEKKSKARRIESLNKALNDIIEKIKNSGIGRKNKILLNNRIQDHLNKIE
                     MVREAYQRLMDYYIKEEIPKERIREKGDKKRRATRRKSFAK"
     gene            complement(12833..13222)
                     /locus_tag="PLK55_02530"
     CDS             complement(12833..13222)
                     /locus_tag="PLK55_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35835.1"
                     /translation="MAKTLAEYIREREKDIETISKSMQRGLKDLLSAQTEKEIKKQLD
                     YLVFLGKSLDALTKKIQGAGIGDRNKRKLLEKISFELKKVNATRQGYEEALTYHLKEE
                     IPKERLKEKGDKKRKVRRIIKRKANLR"
     gene            complement(13236..13886)
                     /locus_tag="PLK55_02535"
     CDS             complement(13236..13886)
                     /locus_tag="PLK55_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35836.1"
                     /translation="MTQKYTEIIQLVHPLFDTFFARDYGIKFRTHQDLLNHINNNKDE
                     LIAINTTLKAYKRAINVIKEKRPTALFVMVLPAEEDKFSGKEPVAAYNKVIKQLIEHS
                     KETLGERAVVTDFHPEYAGHTEYIKPALYSKLNKDLKISSFGEYGNACVSAWLGYANE
                     KLIKKGFNVKKKNILFNYSINNTFADREAKPTYLDRKIIRKQELYKKRTVRKSIAK"
     assembly_gap    14041..14140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14141..15255)
                     /gene="glyA"
                     /locus_tag="PLK55_02540"
     CDS             complement(<14141..15255)
                     /gene="glyA"
                     /locus_tag="PLK55_02540"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOZ35837.1"
                     /translation="MNKITELIENEKIRQQETVEMIASENICSKEIREACGSVLTNKY
                     AEGYPGKRYYSGCEVVDEIENLAREKAKELFKCKYANVQPHSGSSANLIAYFSLLEKG
                     DKILGMSLDSGGHLTHGFKLNFSGRLLESYSYGTDENGFLDYDEIERQAKEIKPKMIV
                     CGASAYPRKIDFKRFKEIADSVDAYLMADMAHIAGLVATDLHESPLPYADIVTSTTHK
                     TLRGPRGGLILTDNVEIGEKINKNNFPGFQGGPLEHIIAGKAICFEEALKPEFKVYQE
                     QVLRNCKTLAEELQKYGFKLVTNGTDNHLILVDLRNKNLTGKKAQEILEEINISVNKN
                     GIPKDPQKPFVTSGIRIGTPAITTRGFKEQQIKVIAD"
     gene            complement(15264..16223)
                     /locus_tag="PLK55_02545"
     CDS             complement(15264..16223)
                     /locus_tag="PLK55_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35838.1"
                     /translation="MNNTKFLDIVICGPDMSGTSTQISDFINYFKDKGKIVRDIRGTE
                     MDLLFHAEVFEKYNKYFMSLKDFLNSDNFSKEEKSNLLLEIYSILSGYQKQADMFVAS
                     FKKNKITTYINPNSADIWIMEEPTKRGAGQVNRVIEQNRSQFDFKETIDGYSAALVHQ
                     AYRTDEFYRIRKILRENNKIIIRSRSEESACYQVYDKKNNTKGIKLKEYLSLPGHKIA
                     FGFPPTHLFVVCGPRSWKKEKYLKLKEERSNGRILDDHELNSDYQLFVNKRYASVWLD
                     KLYQEGCKINGSEYLPVIYRFNIYDSKEKVKKKMYEKLDEMLK"
     gene            16310..>17076
                     /locus_tag="PLK55_02550"
     CDS             16310..>17076
                     /locus_tag="PLK55_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOZ35839.1"
                     /translation="MNILLICSKTDLAAVNFFETLTTESFGFKKLLENLWFLNKKNKI
                     YLTLTEKTHLYMSDKDISKIEKEADTEFEKIIFLSRHSTLGENKPRSITAHSVGNWGK
                     AELGGIDETLVKTDAILIRALLLNLYLKKPRALEYEVKQEATHHGPFLEKPTIFVEIG
                     SNEMAWKDKVVCDFVSKELIKVISDYDYKETKDKNNWQEVVGVGGSHYCTKFNRFTFN
                     LENKYCFGHIIPDYALKQITDTPKLKELLECAKEKSNS"
     assembly_gap    17077..17176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17177..18309)
                     /locus_tag="PLK55_02555"
     CDS             complement(<17177..18309)
                     /locus_tag="PLK55_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35840.1"
                     /translation="MIKRILVLASQRIKSQEAIAFFSKYPSKYKISALMCTDEKQLEL
                     FKKQIKEIKPDVVFFPSKEGTDQIIEELNTRCFYDYTQYAQFLKASNCDLVVSDLTGI
                     DSIKLILATVGEYKDICFLNLEPILYSGKIIINEARNKGITLNFITHQFYSIDLFQKQ
                     KNNLDNINKIILTSFDNEYDKEELINYTHPSKPLSEFKKILYYKNKMWLPRHLMAFSN
                     FYDLDLDKFEYYKSNTSQVSVILQLNNGSNCMINTENNKELIFEYYYPRNTKNSNRDI
                     EKTIEIKLQKIKSTDIQSLDLATQAMAKGGTYPILYQMVYDLCAEAIYNNRTKDKNLF
                     FKVFQEILKDKTFYSKNINIQTIFALYEKLKIKIETEYLKKEK"
     gene            complement(18900..21056)
                     /locus_tag="PLK55_02560"
     CDS             complement(18900..21056)
                     /locus_tag="PLK55_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048060960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HOZ35841.1"
                     /translation="MQNTKNIRNIAIIAHIDHGKSTLTDNLVATAGLISKELAGQQVF
                     MDSYILEQERGITINASNVSVPCEINGEKFLVNIIDTPGHVDFGGDVIRAMRAVDGAV
                     LVVDAVEGVMPQTETVLRQALKERVKPVLFINKVDRLITEMNVAPEDMQKRFVKIVMD
                     INNLVSKNAPEEFAQKWQVNIKDGSVCFGSGYHNWALSVPAMTKYNIKFSDIVDYCKQ
                     EKQKELAQKIPVSEVFLEMVSQHLPNPMQSQKYRIPIIWGGDMETVEGKAMAECNHSE
                     DNIAMMVTDVVLDKHAGDVASGRIYMGTIKKGMDLNVLGADKTAKVQQVGIYLGPERV
                     ILDEVVAGNIVCVIGMKEVYAGQTLSNKEIKPFESFMSDVEPVMTVSIEAKNSKDLPK
                     LIEVVHNITKEDPNIRAELNQETGEHLISGMGELHLEITQYKIEKDNNIPITVSPPIV
                     LYYETIQKESETIEGKSPNKHNKFKISVEPIAPEMLAKIKAMKFDGRIRINKDVEIQN
                     KLVDAGFTKDESKNIWAVHNNCILINRTKGIQNLFETRELVIDAFMDATKEGPVAKEK
                     VSGIIVNLEDATLHEDAIHRGPAQVLPAITRAIWACILSADYTLFEPKQKLSVSVPQD
                     FMGAVNRVLSGRRTQIERIDMEGDSCVVVGNIPVKSLIGLSQDMRGETQGRAIWTAEY
                     AGYEKIPKSILMSTITEIRTRKGMEPEPKSAEFFMD"
BASE COUNT         6477 a         3368 c         2798 g         8128 t
ORIGIN      
        1 cttgcatcat tatgacacaa ataaatcatt cttttataag ttagataata acccatctta
       61 tttagtgctg gcgcgacaag cccctgccat cttgtagctc ctatctttac accatctgta
      121 aaatgacttg cacgagccga acccgcataa acataacttg ttgcaaattc catatctatg
      181 gaatcgtctt gatcatatgt tagatacatg tctgctaaga atggtctgtt cgcagttttt
      241 gatttattaa ttattaaatt tgtattgtgt ctatttattg ctatagaaat ttctaaagct
      301 gtttctggat catttgcaat acccaatagt gatgattggt atttactaaa tgttgaatat
      361 ttagaactcc aattttttaa tttttgtagt tctgatttta tagaattata gcttccgcgg
      421 tcattccaac caggtatatg aatttgtgca acacctgtgc tgccacaaac ttcgccacaa
      481 cccactttta cattacagcc ggattccgca gcaattcttg accacaattg agctgtttct
      541 tcctctgata atccttcttg ttttgcaatc ttaaagacta cgttattaaa tgcttcttca
      601 taacatttat tgttagtgtt atatttttca tctattaaaa aatttccaag gaatgggcca
      661 tttttacgta ttattttacc gtcagaaata aaattatatt tcctagataa atttactgtt
      721 tctatatttc ttcttaaaca ttctatatat ttttctaagg tttcttgctt tgttatacta
      781 caagttcctg taaatgttaa agtttcagca gtatttactg gtggcgaggt ttctactgga
      841 ggtgtgggcg taactgaggg cgtttctact ggaggcgttg gagtaactgg tggtgttggt
      901 gctccaggta ttgcatctac tggtattcct tgaatgcctt ctaatttact tgcattaaaa
      961 tcccatctat ctatacaact tcctgggact ttacagtcat agcctttaca ataaaaatta
     1021 ataatgtttc tatttatctc acataattga tatattgatt gagcattata atctagatga
     1081 tataaatttg tatatgctaa atttattaat gaccctgcat cagacaattg cataaatatt
     1141 aaaacaaatt tatcattaat tttaggatct attgaaaact taaatgtttt atctggacct
     1201 aatgtgtatt ccatagctgc tgggtttgtc gttagtgtgt tagagctaat tctaaaattt
     1261 gattgtaaag tgtaatcttt aaagtctcct gttgatttaa catttgctaa atacccgtat
     1321 atcttatctg aatgatctgg tgttgctata attgcatata gtggtttagt ataattaaag
     1381 cttgatagat ttacgcttat gcttttttta tcgtttctat aatctattag atatgcttct
     1441 gggtcattta tttttcttgc attgtaccag gtatttgtag tactgtattt tacatctgga
     1501 attgtagaaa atactgtaaa atctaattta atttgttgtg aagcaactgg ttctggttga
     1561 ttttctggtt gagcagaatc cccctcactt gaatcacttg attctccatt attaacaaca
     1621 ccttcatttg gtgtggcttc ggcaggagga gttccactag aaaatggatt agttggtatt
     1681 tgtaattcag taacatatcc aaattcatag tttattttat ctaaatatcc tgtattataa
     1741 aaatcagttt ctggaattgt ccatttttta ttatctgctc ctaaaccaag attaatttta
     1801 tttccagaat ctttagattg ccaaacaaaa atatttaatt tctttcctgc aaaaacttta
     1861 gataaacaaa atacccgatt taatccattc ttttgaacat taaatttatc tgctttaatt
     1921 aaagaattat tgccagaaac ggttaaccac tctatttttg aattagaatt agacatagtt
     1981 cctggagttt tataattata aattaaatct aaaatgtctt ttttatttgc actaaannnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgg agttttataa ttataaatta
     2161 aatctaaaat gtctttttta tttgcactaa atgtatttgg gcctacaaaa ccaatatatg
     2221 tttcgctaac atctaatcct gttgtgccac cagatattct taagtgatat ggataatcag
     2281 aattagtaca taacccatca ttcactaatt caattgggtc atttattgac aaatctctat
     2341 aaccctctat ttttcctgaa tatataggat ttaatttact ataactcaat gtaattgctg
     2401 ttgctggagc ggtagcattt ggagacgcct cttgagcacc ttccggatta gatgaagaaa
     2461 aaggcacgcc atatgcataa tctacattat ataaatattt tattttatag tcatactcaa
     2521 gccaccattt gctcttagaa agtccatttt tataaatcaa ttcgttatcc attcctaaat
     2581 cagaatatcc gtcctctcgt ttaatttcgg tccgagtcga tcctttttct gtaacatctt
     2641 ggcacacaaa aacattatct tgttccgtag taccagcgct ttttggttta aatacataca
     2701 catctaagct ttttgaatta cattgttctg aattagatat ataatatttg tgtgttttta
     2761 aattatcacc gggagaaaca caagatgctt ttgaagaacc cgaacacatc tgccacgcat
     2821 tttttccttc ttgtgaacca acaacatata ttccattcat atagcttgca taattctttc
     2881 tatattctaa tggtacagaa acaacaatcc ccatagacgt taatcctgta ccggattcta
     2941 tatttgtatt tattataatt tccccacaat taccatattg gtctgactta tcatacttaa
     3001 ttaaattaat tcctgttatt tttaaatctg aaacaccata atcattatca caccctgttg
     3061 gtttttcacc tttttgtcca ggattctcag caaatacatt tgccgccatt aaaaaaattg
     3121 caaatattac aaaaattgat aaaattattt tttttgaatt catgggttct tccttctttt
     3181 cctttttaaa tttaaatata taacttatac tagttaatta tttgttattt tctataaaat
     3241 atatattctt tagtatttat aaatatattc tttactatat tatttatata taaacatttg
     3301 tatttatttg cttttgttta tttatttgac tgcttgttaa aaggggcgaa actacaatga
     3361 ttgaaattct taaattttct gaacatggca ttcaaagaca tgaaaggcct acaaaggatg
     3421 cttggataaa tgtggttaac cctgacgcct atgaaataaa gtacttggca gaaatctgca
     3481 aaattaaaga agtagactac caagagttta aagatgatat taattctcta caagatatgg
     3541 aagaaatacc atatatggag aaatatagac aagaatattt tataattact agaacaccac
     3601 ataaacttaa aagcacagac ccagaaagca aatataagtc tgctgttgag tatattacta
     3661 tccctttagg aataatgatt aataaagact acattattac tataagcttt agagaaaacg
     3721 aaaatgtaga tattctaaag acaaagaaat ttaaacatga aaaagcaatg gttcttctta
     3781 aattgctttt ggtttcttca aaaacttata tcaaatatct aaaagaaata gatgctaaga
     3841 taaaaaatat tgaagaaaag tttgaaagcg ttcagagaaa caaagaaatt tctgatcttt
     3901 tagatcttaa gaagtctctt gtttatttta atacatcttt aaagagtgat caaatacttc
     3961 ttgaaaagat tgcaagaaat aaagattttg ttaattctga agatgacaaa gatgttattg
     4021 acgatgtaat tgacgagaat aaacaggcaa ttgttgttac tgcaatctat aaaaatatta
     4081 ttgcagatgc aacagatctc tttaattctg ttatttctaa caaccttaac aaaaccttaa
     4141 aattattgac atctattact gttgttattg caataccaac attagttgca agcatctatg
     4201 gtatgaatgt gactttgcca tttgatcaca gcaaatatgc gtttattatt gttatgggaa
     4261 taattgctat tctttcaaca cttggcatag cgatactttg gaaacatgac atgttttaga
     4321 gctaattctt aaaaaaataa aaaaagattt gcccaatatt gggcaaatat actattattc
     4381 tattgccctt taaaaagagc aaccatatct ttttgcttct ggaataaata tattgataaa
     4441 aaaataataa acccatagaa cgcaatcaga gttaaagaaa ttaatcctaa aactggcata
     4501 gtaaataaaa gaataattga aggaataatc cctgttagtg ctaatatccc tgcaaaaatg
     4561 tataacggaa ttaataacat aattcttgcg tagtttttat aggtcccaaa accataagcc
     4621 actaaaaatg agtaaatcgc aataattatc attataacgc ccacaacacc tgttgcttta
     4681 cttgcaaaac tattaatttc gccaatttca gaaaacattg gatttgcgcc agttaaagaa
     4741 ttcaacattg ttggcgaaat aaaaattaat atcccaaaaa ttacaaagat tgctcccgcc
     4801 ccccaatgcg aaattgcaac taattttgca atatattgac ttaaagataa ctctcctagt
     4861 tttttcataa atttcccccg tttataattt atattactta attgatatat cttttagttt
     4921 aaatatcttt ctttttccca gatacaaaga aaatcgtctt cttctcaaaa atatttgtaa
     4981 tctctaattt ttcatttctt atatttctgt gtccaattaa agagatatga ttctctttaa
     5041 accaaccttt tacttctctt aatgtgtgat aactttcatg tggcacagca taacgatcat
     5101 aaagagttga ttttggtgct ttctggtttt tgttaatttt gtaaacaaaa gctattattt
     5161 tatctggatc ttttgaaaca agattacgaa gaaaaaatcg ctgtaaacga tatttggttc
     5221 tagagtattt gtgatatagc ccaataataa ttatgccttt cctttttagt tgcccaacaa
     5281 ttcgtgaaaa agattttttt ggattttctg tgtgatggag gacaccaaga caaactatta
     5341 tatcaaattt gccaagctct tcgagcgggt attttaaaat gtctttttct ttaaaaacta
     5401 ggtttttaac tttatcttta tttgccttct ttcttgactc ttcaagttga ccttttgaaa
     5461 agtcgattgc tacaacctct ttagctcctg cttttgcaaa aaaaatagat ttttcgccag
     5521 ttccacaacc tgcatcaagt attcttttgt cttttaggtc tttttcttta aggccagcag
     5581 tctcaaggat gcctaatatt atctttttat ttgttctttt ctgattagaa gtatattggt
     5641 tagctttagg atattgatat ttattataaa actctttaac tgattgcata attatgagat
     5701 ttagaaatat tttattgagc ttatttcttc ttttttctag aatatgcttt tatctttgac
     5761 catagagaca ataaaacaat gccagaaaca gcacctgccc caacacctgc aatattacca
     5821 aatagtttag attctttctg cacaaaattt agaacttctc ttgattgtct tggatttttt
     5881 gaatcttcgt tatagatttc taaacttttt attacttcgt ttaatccttt aattacttta
     5941 gggttattac caaatcttgc agcaaattct tcaacagata aactagacaa ataattaatt
     6001 cttgctgtat cgtttagttc tttcatttta agcgcaactt cttcagagaa tttgtcacta
     6061 gtctttaatt cttttacaat atctgcatat atattgtcta gctgtgcctt ctcagaagaa
     6121 tataatttat catatatgta tcttaatttt gcaacgccta aatcaagttt agaaatttta
     6181 tctccattcc aaaaatatac ttcttctggc tttgcgtttt tgtttaattc agttactttc
     6241 ttctcaataa ttttatctaa tctttcaaag tttgcatttt gttttttttc tatctctgtg
     6301 gcttttttta catccatttc taaagctaca attttatttt gtttttcatc ataaaaatat
     6361 agttttcttt taatttcttt gtttttactt cttaacaact tctgtgaata ttcttttttt
     6421 gcattagtat ttaattcagt aattctttga ccaacagatt taatttcaga aagataattg
     6481 ccttttgcca aaactatatt ttggtttaca attgatggtt tagttgcaga atatactttc
     6541 tttcccacat aataagatgg ggcagctgct agcgttccaa taagtgcacc catcgcaact
     6601 ttctttccta ccttttttgc agttattcct attctactac caattgttga ttttggttta
     6661 ggttttggtg acataattct tccctcttaa atatttattt tctgcttaaa gacaaaagaa
     6721 caaaacactt tatatttgtt ttctttttta atgagtatat ggctttatta aaagagcaag
     6781 aaaaactatc ttttcttctt gaaaaatcaa ttgcaaatct tacaaaagat aaatctaaaa
     6841 gctatggttt acttttcagt ggcggtgttg attcttgtct tctggctctt cttttaaaaa
     6901 aacaaaaagc taagtttact tgtttttttg catatgttaa agataatacc gaggccaaag
     6961 acctggcttt tgcaaaaaaa gcagcaaaag aacttgattt aaaacttgaa ataatctctg
     7021 cggacagaaa agagatttca actattcttc ctaagattat tgatataatt gattctacta
     7081 atcctgtttc tgtgggaatt gcaattccga tttatctctc ttctagaaaa gcaaaaacca
     7141 aaaagattga tacaattttt tcaggtcttg gttctgatga gctgtttgct ggctatagca
     7201 gatttactac ttataatcaa aagaccgaaa caaaaaaatg ccttgataat attcaaaagg
     7261 acaatctcga tagagatgag gcaattgtaa aacattttaa attagcaatt aaatgcccat
     7321 atcttgataa agcagttatt gattatgctt taactttgcc tccaaggctt tttatttctt
     7381 caaaacaaaa taaaataatt attcgtaaaa ttgcaaaaaa cttagggctc tctacagggc
     7441 ttgcagaaag aaaaaaactt gcttgtcaat acggtagtaa ttctgataga acacttaaaa
     7501 aacttgcaaa agctaaaaaa tttaaaacaa aaagcgaata tctttcttct tttaaaaaag
     7561 aaaaagttgg cgtgcttttc tccggaggta aagactctaa tctttcatta tggcttgcac
     7621 aaaaggacag aaaagagatc tcttgtctta tctctttaat tccagagaat gttgactcct
     7681 atatgtttca aacaccagaa gttaatttcc taaaaatgca ggcttttgct ttagatatac
     7741 ctcttcttgt tactaaaaca aaaggacaaa aagaaaaaga acttgttgat ctcagaaaag
     7801 caatagtttt tgctaaagca aaatttggat taaatggaat tgttgctgga gctctttata
     7861 gcaattatca aaaggacaga atagaaaaga tctgtaaaga tctgaattta aaattgattg
     7921 cgcctctttg gcatatgaaa gaagaagatg aaataaaatt gcttttagat aataatttta
     7981 aatttattat atgtaagatt gctgcttttg gtttttctaa agaaatgctt ggtaaagtaa
     8041 ttgattacaa agatttagag gaattaaaaa aactaaatag aaaataccaa acaaatattg
     8101 caggagaagg gggagaatat gaaactcttg ttcttgatac tccgatattt aaaaaaagaa
     8161 ttatcatttt agaaagccag aaaaccatgc aaaatgaatt tacaggaaac ctaattatta
     8221 aaaagataaa actagaaaaa aaataaattt ataaactatt tgagcttaat tttgatagaa
     8281 tatggcagca attttagcac acatgcaatt tgtaagagat gcagttaaag aaaaccctaa
     8341 acttttttca aaatctgatt taaaatatct actacagggt gctacatatc ctgatattta
     8401 ttatattact ggccttagaa gcataactaa aaaacctaat tttagtaaat ttgttcacga
     8461 aaccatggat gaagattata gctttgcaaa attattactt gaaagagcaa aaaatcaaca
     8521 agaaaaactt tttgcattag gctttatatc tcattttgtt ctggataaac acattcataa
     8581 ttatattaat agtacagaac tcgcgcatag tgtaaaacat ctggtttcag aatattattt
     8641 agatacaaaa tttaaaaatc aaaaaatacc tatccctaga tatcctttaa aattaattaa
     8701 aacttgtttt aaagaatatt atcctatgga ttatgagaag tttaaaaaaa gaataaagct
     8761 atcaataaac agcataattt tctatgattt tgcaaataga tttctaatta agaaaatagt
     8821 aaatgcaaga tatagaaaag gaaaatataa aacaagatta tctcttctaa atgtcccttt
     8881 taaactcgca agactatcta aatatcaaaa attcggatat gattataggg ctttattaaa
     8941 cccagatatt tctattaaaa tagaacacac agaaaaaatg tataaattat ttttaaaagc
     9001 aaaagaagag tttatagatt tggttattga ttatgaactt cacatctcgg attatacttc
     9061 taaacaaaag agaattatag atttcggata aatcagaaat cagaaaacgc atttttagaa
     9121 ttctgtttat catcaagttg ataatatttt tttgtaattc tactatttct ttttttagtt
     9181 cagaaatctt tttttatcta attttgattc tgaaattaag cgcttaatat ctatctgtaa
     9241 aattaaagtc tgtctttaaa tcttttttat tattttttaa ctatctttta agtttggtgg
     9301 agctaatata tctagaagta ctagatagat acctgactgg ctttttagga tttataggca
     9361 tttagatcac agttattgta accgtagttt cggtcttatt gtctataaca ttatagtcgt
     9421 tattgtgttc tccgactctt atctttattt tttttgttcc gggatatata cctaccgctg
     9481 gataaataga atatcttatt tcctttactt gcccaggatt aaattcgcca attcttttgt
     9541 aatctgtgtt aattggctta acagaattaa aacttaaatc ttcgtcaata actaaatcaa
     9601 tacttaacat cttcttgatc tggctatttg ttaatttaat ataaagctct aaaggctctt
     9661 tacttttaga atctaatcta taagggtatg ttctagtatt tattccaatc atcttattac
     9721 caccatttta attataataa tacaatagat aaaagaaaat ataaatgttt tctagatggt
     9781 tattttttaa aaagcttttt gagttttgct ttagacatct ttttaagacc gtattttttt
     9841 aaatgatcta attcaagggt ttctaatttt tcaaggtata atttatttat atacatattc
     9901 cccactcttt ttcatttata ttttgttccg tatttttctt tgtcatcaat aatgtcgttc
     9961 tttttccaat cttctaccca atcatttact tcaaatgatt tatatttgcc atctgctgtt
    10021 gaacacacta ctcttttgat tccagcattt attatcatct tcttacagat aaagcaggga
    10081 aatgcgtcaa ttggtttttc taatccctga tatatctttt ttccataaag atataacacc
    10141 ccatcaaata ttgaaactcc atctcttgct gcatttatta ttgcgttctg ttcagcatga
    10201 acactaacac aaagctcata ttgctgacct gaaggcactc ctaactttct tctaagacaa
    10261 tagcctttct caaaacaatc tgttgtgcct cttggtgctc ccacatatcc tgttgatttt
    10321 attacattgt cttttacaat aacagaacca aaccttgcag aaaggcaatt acctcttaaa
    10381 gaaacaactt ctgcaatctt taaaaaatat tgatcccaag atggtttgtt tctatttttt
    10441 atttgtattt ttaataataa atcatctact tgtttttcta agtcttttag acttccatta
    10501 ttatttattg taaattctgc aagtttctta cagctttcta gattttgaga atttggatct
    10561 ttactgttta gttctttctt ttctgaattt ttaaagtctt caaaacttga gatgtgttct
    10621 ttctctttta gtctttcttt tgctctttgg tatctagtca ttgcttcagc atctataaaa
    10681 actagattaa aattgggaag tttttttaat tcttctattt ctccttctgt tcttattgat
    10741 tctactacat aattcttgtc aaaaccttga agagtaattc tttcagcaag tctttttgca
    10801 agtatatttg ccccaaactt ctgtcttagt tcatttccta ctctttgaag attgtctctt
    10861 gttatctcta tattgtcttt cttacattct tctctaatct catcagaaca agaatagaca
    10921 ataaaacctt tttgttttaa aatctctgaa actgctcctt ttcctgaagc taaaaaaccg
    10981 gttataccaa ttatcatgtt tttcaccatt tattttctta aatatgttat aaaagaatag
    11041 ccatctttct tttcttcagc aacaatcgtc caagtatttg tgtttatttc tggaaaaaat
    11101 gtatctcctt cgtattcttt atccagttgt gtaatttcta gtttatctgc taagttttgt
    11161 tccattgctt gtttgtaaat gcttgctcct ccaataatat aaacttgatt atctatagtg
    11221 tgtgcataat ctattgcttc atttagacta ttttttatag ttattccttc ttctttccaa
    11281 gaatcatcag gaaaagttaa aactatattg gttcttccag gaagtggttt aattggaaga
    11341 gaaagatatg tgttttttcc cataatcacc ggatgcccgg ttgttagtct tttaaatctt
    11401 ttaaagtctt ctgatatatg ccaaggaatc ttgttgtctt tacctattgc ataattcttt
    11461 gcaattgcta caataattat tgtttctttt tccattattt ctcttttcct ttatatttaa
    11521 actgcgattt caaattttat ggttggctgt ggtttatagt caataatctt agtatctgta
    11581 tatttccaat caaaaataga agtaaaattt tctgtttcaa tttttggaag aacccctggc
    11641 gttcttgaaa tttgttcttt taagccgtca acatgattta cataaatgtg tgtgtctcct
    11701 aaaaaaccaa ttagctttcc ttctttaagg ttagcttctt ttgctaaaag atgtaatagc
    11761 aatccgtagc ttgctatatt aaatggaaga cctaaagcag aatcaactga tctttggttc
    11821 cataaaagat ttaattttcc atttattact gttacttgaa agccataatg acatggtggc
    11881 aaagccatgt gtttaatatc taaagggttc caggcagaaa caatcattcg tctgtctgtc
    11941 gggttggttt ttagagtatc aactagtttt tttagttgat caaccccctc accagttggc
    12001 tctttatcaa atgattgata tttttttcca aagtttcgcc attgatagcc atagattgga
    12061 cctagttctc tttcttcaag cattttcttt tttgtttctt cgtcattacc ataaggcaca
    12121 acatctggag aacaccattc gtcccagata tgattatttt catttcttag ccaattttta
    12181 tcagttattc cttttataaa aaattcaagt tcgcttgcaa ttaatctaaa aggaactttt
    12241 ttagttgtta aaagcggaaa cccttctgac atatagtgtt caaacataca accagcaaca
    12301 gcaattgtgt caattcctgt tctattgtgt tttagttctc ctttttccac aatgtttctt
    12361 ataatatcaa gatatgctct catatttttt cactcctttt attttgcaaa actctttctt
    12421 cttgttgctc ttcttttctt atctcctttc tctcttattc tttctttcgg tatttcttct
    12481 ttaatataat aatccattaa tctttgatat gcttctctaa ccatttctat tttattcaaa
    12541 tgatcttgta ttctgttatt taatagtatt ttgttttttc ttcctattcc tgaatttttt
    12601 attttttcaa taatatcatt tagtgcttta tttaatgatt ctattcttct ggcttttgat
    12661 tttttttcat ctatagttgt gctaacatat acttcactta atgtatccaa tatacttttt
    12721 cctataaaat caatcattct ttcttgttct ttaatataaa ctgttaatgt ttttgatttg
    12781 tttttattaa cttctgcttt tgctttctga aaatcaatta tctttgccat atttatctta
    12841 aatttgcttt tcttttaatt attcttctaa cttttctttt tttatcccct ttttctttaa
    12901 gcctttcttt tggtatttct tctttaagat gataagttaa agcttcttca tagccttgtc
    12961 ttgttgcatt aacttttttt aactcaaaac taattttttc aagtaatttt cttttatttc
    13021 tatcaccaat acctgcgcct tgtatttttt ttgtaagggc atctaaagat ttacctaaaa
    13081 aaactaaata atctagttgc ttttttattt ctttttctgt ttgagcactc aataaatctt
    13141 ttagccctct ttgcatagat tttgagatag tttcaatatc tttttcgcgt tcgcggatat
    13201 attctgctaa tgtttttgcc aaatctttca cctttttatt ttgcaataga ttttcttact
    13261 gttctttttt tgtataattc ttgctttctt attatctttc tatccaaata tgttggtttt
    13321 gcttctctgt ctgcaaaggt attattaata ctataattaa aaagaatatt ttttttctta
    13381 acattaaatc cttttttaat taatttctca tttgcatatc ctaaccaagc cgaaacacaa
    13441 gcatttccat attctccaaa ggaagaaatt tttaaatcct tatttagttt tgaatataat
    13501 gctggtttta tatattctgt gtggcctgca tattctggat gaaaatcagt tacaactgcc
    13561 ctttctccaa gtgtttcctt tgagtgctca attagctgtt ttatgacttt attataagct
    13621 gcaacaggtt ctttaccact aaatttatcc tcttctgccg ggagaaccat aacaaataat
    13681 gcagtaggtc ttttttcttt aataacatta attgctcttt tatatgcttt taatgtagtg
    13741 ttaatagcaa tcaattcatc tttgttattg tttatgtgat ttaaaagatc ttggtgtgtt
    13801 ctaaatttta taccataatc tcttgcgaaa aatgtatcaa acaatggatg aactaactgt
    13861 attatttcag tatatttttg agtcatattt tatctattgg atatgctttt aacatttcaa
    13921 ttacttttac ttttagattt acttttaact cttggttttc tggtttcgaa attacttgat
    13981 taatataatc tgcaatgact tttatttgct gttctttaaa gccacgggtg gttattgcgg
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taatctgcaa tgacttttat
    14161 ttgctgttct ttaaagccac gggtggttat tgcgggtgtt ccaatcctta ttcctgaagt
    14221 aacaaatggc ttttgtggat cttttggtat tccgttcttg tttacagaaa tatttatttc
    14281 ttctaagatc tcttgggctt tctttcctgt aaggtttttg tttcgtaaat ctacaagtat
    14341 taaatggttg tctgttccat tcgttacaag cttaaatcca tatttttgaa gttcttctgc
    14401 taaggtttta cagtttctta gaacctgctc ttgataaact ttaaattctg gttttaaggc
    14461 ctcttcaaaa caaattgctt ttcctgcaat tatgtgctca agtggcccgc cttgaaagcc
    14521 aggaaaatta ttcttgttaa tcttttctcc aatctcaaca ttatctgtta aaattaggcc
    14581 acctcttggg cctcttaaag tcttgtgagt agttgaagta acaatatctg cataaggcaa
    14641 aggcgactcg tgaagatctg ttgccacaag gccagcaata tgtgccatgt ctgccataag
    14701 ataagcatca acagaatctg ctatttcttt aaatctttta aaatcaattt ttcgagggta
    14761 agcagaagca ccacaaacta tcatttttgg tttaatttct tttgcctgcc tttcaatttc
    14821 atcatagtca agaaaaccat tctcgtcagt tccatagcta tagctttcaa gaagtcttcc
    14881 tgagaaattt aatttaaaac cgtgtgtcaa atgccctcca ctatcaagag acattcctaa
    14941 aattttatcc cctttctcaa gaagagaaaa ataagctata aggttggctg aagaaccaga
    15001 atgcggctga acattagcat atttacattt gaaaagttct tttgcttttt ctcttgccag
    15061 attttctatt tcatcaacaa cctcacagcc agaataatat cttttaccag gatagccttc
    15121 agcatacttg tttgtaagaa cagaaccaca tgcttctctt atctctttag aacaaatatt
    15181 ctcagaagca atcatctcta ctgtttcttg ctgtctaatc ttttcgtttt ctattagttc
    15241 tgtaattttg ttcataaata atcttattta agcatttcat ctaacttttc atacattttt
    15301 ttcttaactt tttccttaga atcatagata ttaaatctat agattactgg gagatactct
    15361 gacccattta ttttacagcc ctcttgataa agtttgtcaa gccaaacact agcatatctt
    15421 ttgtttacaa acaattgata atctgagttt agttcgtgat catcaagtat ccttccatta
    15481 cttctttctt cttttaattt aagatatttt tcttttttcc aggatcttgg tccgcaaacc
    15541 acaaaaagat gtgttggtgg aaacccaaaa gcaattttat gacctggaag agaaagatat
    15601 tcttttagtt ttattccttt tgtgttgttt ttcttatcat atacttgata acatgctgat
    15661 tcttcgcttc tgcttctaat aataatctta ttattttctc ttagtatctt tctaattcta
    15721 taaaattcat cggttctgta tgcctgatga acaagagctg cagaataacc atcaattgtt
    15781 tctttaaaat caaattggct tctgttctgt tcaattactc tatttacttg gcccgctcct
    15841 ctttttgttg gttcttccat tatccagata tctgcagagt ttggattgat ataagttgta
    15901 attttgtttt tcttaaagga agcaacaaac atatctgctt gtttttgata acctgaaaga
    15961 atagagtaga tttctagaag aagattgctt ttttcttctt ttgaaaaatt atctgaattc
    16021 aaaaaatcct ttagagacat aaaatattta ttatatttct caaaaacctc tgcatgaaac
    16081 aaaagatcca tttctgtgcc tcttatgtct ctaacgattt tgcctttatc tttgaaataa
    16141 ttaataaaat cagaaatctg tgtgcttgtt ccagacatgt ccggcccgca aataacaata
    16201 tctaagaatt tggtgttgtt cattttagac cccaaaatat aataactagt aaactgttta
    16261 taaattgttt ctacaaatat ttatgtatag acaaaaaatg ctgatttata tgaatatctt
    16321 gcttatttgt tcaaaaaccg accttgcggc tgttaatttc tttgaaacat taaccactga
    16381 aagctttgga tttaaaaaat tattagaaaa tctttggttt ttgaataaaa aaaataaaat
    16441 ttatttaaca ttaacagaaa aaactcatct ttatatgagt gataaagata tttctaaaat
    16501 tgaaaaagaa gcagatacag aatttgaaaa aattattttt ctttcaaggc actctactct
    16561 tggagaaaac aagcctcgct caataactgc tcactctgtt ggcaattggg gcaaagctga
    16621 acttggagga atagatgaaa cgcttgttaa aactgatgct attttgatta gggcgttgct
    16681 tttgaatttg tatttaaaaa aaccaagagc tctagaatat gaggtaaaac aagaagcaac
    16741 acatcatggg ccttttctag aaaaaccaac aatatttgta gaaattggtt caaatgaaat
    16801 ggcctggaag gataaagtcg tttgtgattt tgtttctaaa gaattgataa aagtaatttc
    16861 agattatgat tacaaagaaa caaaagataa aaataactgg caagaagtag ttggtgttgg
    16921 tgggtcccat tactgtacaa aatttaatcg ctttactttt aatcttgaaa acaaatattg
    16981 ctttggacac attattccgg attatgcgct taaacaaata acagacacac ccaaattaaa
    17041 agaactgcta gaatgtgcaa aagaaaaaag taattcnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnttct tttctttctt tagatattcg gtttcgattt ttatttttag
    17221 tttttcatat aaagcaaaga tagtttgaat gtttatgttt tttgaataaa aggttttatc
    17281 tttaagtatt tcttgaaata ctttaaaaaa taaattcttg tcttttgttc tattattgta
    17341 tatagcttct gcacaaagat catagaccat ttgataaagt ataggataag taccgccttt
    17401 agccattgct tgtgttgcta aatctaaaga ttgaatatct gtacttttta ttttttgtaa
    17461 tttaatttct attgtttttt ctatatctct attactattt tttgtatttc ttggataata
    17521 atattcaaat attaactctt tattattttc agtgttaatc atacagttac taccattatt
    17581 cagttgtaat atcacactta cttgtgaagt gtttgattta tagtattcaa atttgtctaa
    17641 atccaaatca taaaaattac taaatgccat tagatgcctt ggaagccaca tcttgttttt
    17701 atagtatagt atttttttaa attctgataa cggtttagaa ggatgtgtgt aattaattag
    17761 ttcttcttta tcatattcat tatcaaaact agttaatatt attttattaa tattatctag
    17821 attgtttttc tgtttttgga ataaatctat tgaataaaat tgatgagtaa taaaatttaa
    17881 agttatacct ttgtttcgtg cttcattaat tataatttta ccagaataaa gtattggctc
    17941 aagatttaga aagcaaatat ctttgtattc tccaactgtt gcaagaatta gttttattga
    18001 atcaattcct gtaagatcag aaacaaccaa atcacaatta cttgctttta gaaattgagc
    18061 atattgcgta taatcataaa aacatcttgt gtttaattct tcaattattt ggtctgtgcc
    18121 ttctttactt ggaaaaaaaa caacatctgg ctttatttcc tttatttgtt ttttaaaaag
    18181 ctctaattgt ttttcatcag tacacataag cgcagaaatc ttatatttgc taggatattt
    18241 agaaaaaaaa gctatggctt cttgtgattt aattctttgg cttgcaagta ctaaaattcg
    18301 ttttatcata attatatata agatgaaaag tctttaaaaa agtagttttt ttgatttttt
    18361 gaaaataaaa aaaagaaagt aaaaaaagtg ttaattaaaa aaatactttt ttactttcta
    18421 ggttattaaa tttatttctt ctttgctgct tttttctttt ttgctgccat ttttcttccc
    18481 cccttttaat attaagatta aaattatcta tgtatatgta agatagatat ataaagctta
    18541 ttattggata tttgtccaat atatcttaca tgtttttagg gtctattttg agggcctttc
    18601 tggagaaatt gatttttggc ttgtccccag acaaataaaa taattttgtc caattgattg
    18661 tgtgttgtcc aagaaatcgt gtaccacgga aagacattta aataaaataa aaatagaaaa
    18721 atataaaaaa aagaaaagaa caaactgttc ttttggggat gcacaataaa atttgttttt
    18781 attggcacag agaaatcata aagtgatttg tcttgtgaag gcacttttct ttaaagctca
    18841 agaaaacaaa agttttctga gcccagaaaa atcctttgat ttttcttgga aaagggtctc
    18901 taatccatga agaattctgc agactttggt tctggttcca ttccttttct tgttctaatt
    18961 tctgttatgg ttgacattaa aattgatttt ggaatctttt cataacctgc atattctgca
    19021 gtccagattg ctctgccttg tgtttctcct ctcatatcct ggcttaaacc aattaaagat
    19081 ttaacaggaa tatttcccac aactacacaa gaatctcctt ccatatctat tctttcaatc
    19141 tgcgttcttc ttccagataa aactctattg accgccccca taaagtcttg aggaacagaa
    19201 acagataatt tctgttttgg ttcaaataat gtataatctg ctgaaagaat acatgcccag
    19261 attgctctag tgattgctgg aagaacttga gcaggtcctc tgtgtattgc atcttcgtga
    19321 agagttgcat cttctaggtt aacaataatt cctgatacct tttctttagc aaccggtcct
    19381 tcttttgttg catccataaa cgcatcgatt acaagttctc tagtttcgaa aagattttga
    19441 ataccttttg ttctgttgat tagaatacaa ttattatgta ctgcccaaat attctttgat
    19501 tcatctttag taaatccggc atctacaagt ttattttgta tctcaacatc tttatttatt
    19561 ctgattctac catcaaactt catagcttta atttttgcaa gcatctctgg agcaattggt
    19621 tcaacagaaa tcttaaattt gttatgttta tttggggatt tgccttctat tgtttctgat
    19681 tctttttgta ttgtttcata atatagaaca attggaggac ttacagttat tggaatatta
    19741 ttatctttct caatcttata ttgagtaatt tccaagtgaa gctctcccat acctgaaatc
    19801 aagtgctctc ctgtttcttg gtttagttct gctctaatgt tagggtcttc ctttgtgatg
    19861 ttatgaacaa cttcaattaa ttttggaaga tctttactgt tctttgcttc aatagaaaca
    19921 gtcattactg gttcaacatc tgacatgaag ctttcaaatg gttttatttc tttattagat
    19981 aatgtttgac cagcataaac ttccttcata ccaataacac aaacaatatt acctgcaaca
    20041 acttcatcta agattactct ttcaggacct aagtaaatac caacttgctg aactttagca
    20101 gttttgtctg cgccaagaac attaagatcc attcctttct ttattgttcc catatatatt
    20161 cttccagaag caacatctcc tgcgtgttta tcaagaacaa catctgtaac catcattgca
    20221 atattatctt ctgagtgatt acattcagcc attgcttttc cttcaacagt ttccatgtct
    20281 ccaccccata ttattggaat tctgtatttc tgtgactgca tagggtttgg taaatgctgt
    20341 gaaaccattt ctaagaatac ttcacttact ggaatctttt gagcaagttc tttttgtttt
    20401 tcttgtttac agtaatcaac aatatcgcta aattttatgt tgtatttagt cattgctgga
    20461 actgataatg cccagttgtg atatcctgaa ccaaaacaaa cagaaccatc tttaatatta
    20521 acttgccatt tttgagcaaa ttcttctggt gcgtttttag atactaagtt attaatatcc
    20581 attacaatct ttacaaatct tttttgcata tcttctggtg caacattcat ttctgttatt
    20641 agtctatcta ctttgttaat aaatagaact ggtttaactc tttcttttaa tgcttgtctt
    20701 agaacagttt ctgtttgagg cataacccct tcaacagcat caacaactaa gactgctcca
    20761 tcaactgctc tcatagctct aataacatct ccaccaaagt caacgtgacc tggagtgtca
    20821 ataatattaa ctaagaattt ttctccgttg atttcacaag gaacagaaac gtttgaagcg
    20881 ttaattgtga ttcctctttc ttgttctaag atataagaat ccataaatac ttgttgacca
    20941 gctagttctt ttgaaattag tccagctgtt gcaactaagt tatctgttaa agtggatttc
    21001 ccgtgatcaa tatgtgcaat aattgcaata tttctaatat tttttgtatt ttgcattaat
    21061 ttttgtgcaa g
//
LOCUS       DAOOON010000013        18861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_3565_length_18861_cov_20.598851, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000013 DAOOON010000000
VERSION     DAOOON010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 18861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18861
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_18861_cov_20.598851"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLK55_02565"
     CDS             complement(<1..1168)
                     /locus_tag="PLK55_02565"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOZ35842.1"
                     /translation="MDLIISEKAIAGKRIASILSNNNFTTIVENGVNCFSFNFNGKDT
                     LVIPLRGHINTVDFVGDNNYWNIYTLDLLADKQLTYLQVEKGIISSLKKKRDKLENII
                     VATDADREGEAIGVEAYNYVIENNKNTKLHRAYFSALTEKEIIDSFKNLRKVDFNYAD
                     SVFARQEVDLLWGAILTRYLSVVSNRKGKLFISAGRVQTPLLNFIVERELERQKFVPE
                     KYIEIIANFEKTKIKFSGSHKDGKFFDIEKGKKIFENIKNEKTGIVKAVSKKEKTLNR
                     PEPFNTTSFLRAASSVFGISTNQAMNIAESLYQQGLISYPRTDNTVYPASINLKEIVE
                     IVLQDKDYAAVAKEILSKPIHPSRGEKETTDHPPIYPVGYSNKLAGGEKKIYDMI"
     gene            complement(1175..1645)
                     /locus_tag="PLK55_02570"
     CDS             complement(1175..1645)
                     /locus_tag="PLK55_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ35843.1"
                     /translation="MQVIDKIDKYIIEQLAANGRVSFANIAKDINLTDVAIKKRLDRL
                     MQRGIIKSITAEIDYEILGFNEKVEILITIDPSKQNDIIKKLKEMEDIKEVVEVTGEY
                     NVLARVMALDKGDLKTLLDSIFKIDGITKASILTILKEHKNSKNLPRKILQTTF"
     gene            complement(1663..2376)
                     /locus_tag="PLK55_02575"
     CDS             complement(1663..2376)
                     /locus_tag="PLK55_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35844.1"
                     /translation="MSMDIAVAFKKYVSNIKLVFATSLLLIFTLFLVNPLFSLSGGSL
                     NLTYELLNQDPLIIILTLVAFIAIVFAYTLLQTIVVYKMGREYDFENKNSREIKKPFI
                     ELLKFNISFYLLLYVALCILFDFGLVNNVFVNIALFIITLLFWFIPQIIIMEKQNAEH
                     SVFLSLNYIKKKPVYFVYLFVTTFILTLFTYIIDIVFGVFAGPIIAMVFFVMFVIPFI
                     EILKTEVYLDKYDLLKPFK"
     gene            complement(2470..3693)
                     /locus_tag="PLK55_02580"
     CDS             complement(2470..3693)
                     /locus_tag="PLK55_02580"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HOZ35845.1"
                     /translation="MKTIFIKTFGCTLNQRDSQDIVLGIKESKDEKSADIIIINTCGV
                     KEQTETKIYKYIVDHQKEFSNKELILTGCLIDIDFSKLKELLPKAKMFGIKDKAKLIK
                     YLDKFRNLKKKTKAEITKTLIIANGCLGNCAYCAVKFARGKLKSKSSKDIKTEIEEAV
                     NNGAKEILLTAQDTGCYGLDIKTNIASLLKEIITIPGDFRIRLGMGNPQHFKNYKTEI
                     SQIMKSEKMYKFLHVPMQSGNNAVLKKMNRHHTKKEYLDLINYFKKNIKGLTLATDVI
                     VGFPTETEKDFLETVEVLKKCNFDIVNISRFGQRKGIEANNYKDLHGRIKKERSRLVT
                     KICAKIALEKNKKLIGKTKEILLIELGKNNTLIGRTNNYKQVVVPKDKLILGKTSKVK
                     ITAAKPGYLEARPVF"
     gene            complement(3701..4087)
                     /locus_tag="PLK55_02585"
     CDS             complement(3701..4087)
                     /locus_tag="PLK55_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35846.1"
                     /translation="MNKTLKFILFFLLSYVLAQGLFYFLAKENFFTSPMYILLPVVAF
                     FGLYFVMPMVEEYTKWNKWTLFILFIVISVLCYVLIVYIYVYQIYIILNNMKIPKNLE
                     ILKQLMSSSFLGFIVSGAIGIIAHKK"
     gene            4230..5192
                     /locus_tag="PLK55_02590"
     CDS             4230..5192
                     /locus_tag="PLK55_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HOZ35847.1"
                     /translation="MITASAPWTEKYRPQRLDDISGQKHVVSKLKKYVENKSIPNMLF
                     AGPPGVGKTCCAIALAKELFKDGYSQDFLELNASDERGIGVVRGAIKDFARTIALTSS
                     FKIIFLDEADALTPEAQQALRRTMEKYTNTVRFILNANYSSKLIPPIQSRCALFKFKP
                     LTKEDIKDRIEYIVKEEKIKIDKEAIAAINEVCEGDLRTAINLLQTAASTSEHITEKD
                     IYIVSNSAQPQDIINLIDACLTKDFLKARTTLETLLFDHGLSGEDIINHIYKKIIAYP
                     ENQISIDKKLKIIDKIAEFNYRLVQGANDRIQLEALLSQIILIE"
     gene            5203..6972
                     /locus_tag="PLK55_02595"
     CDS             5203..6972
                     /locus_tag="PLK55_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOZ35848.1"
                     /translation="MVSYLDELKAQMAKDIDKSNKSNDKKIEAPPKEIKKEELKTASA
                     TTTSNPETKPKEQPKLDNLKDAFSSVSQELNKVAPKQSPESLKSLMGDIKDEREADSL
                     ISKWERTGTVITKYGDTEVYRVPDEPLFYYKTNIPKPSPSQKLIINTLKEATTRLVST
                     DPYKIRDPKQRQNIYYQKILEILENAPEINVPKHLFDFYAKAVVTEMVGYGVIDTLIH
                     DSKLEEIMIIGPKKPVYIYHRDYGMMYTNIIFYSDDEIQNIIDKIARILGRRVDISNP
                     LLDARLPDGSRVNATIPPASIDGSTITIRKFREDPYTIVDLVNFGTINLEVAAFMWVV
                     VEGLGAKPANILISGGTGSGKTTLLNVLANFIPDSSRVLTIEDTAELKLPIKHIIRFE
                     GKPPGLEGTGELTLEILVKNTLRMRPDRIIVGEIRHLEASTLFTAMNTGHDGCMGTIH
                     ANSARETLVRITSPPMDVPMLMLAGLDFIIVEKRMNTSRGLVRRIVEIAEITGVFENN
                     PQANIIFRWNPQTDNLERTNEPIKFFDIIKMYTKYGDSDIKRILEERISLINQLVKNN
                     IRSINEVSKTVQEVYTNNSKKNE"
     gene            6981..8129
                     /locus_tag="PLK55_02600"
     CDS             6981..8129
                     /locus_tag="PLK55_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ35849.1"
                     /translation="MPEEDKRLKILRKELDKDDSSASKVDLDAIQNIVSKIKDKDRAK
                     GVSGNVSESHLKELRDIISEGKLAKLNVDTEAKLLENSASELDKGAVKIYKAVPGFIN
                     YIVKSIIKRSIGRKIAFYLYSADIKKTLVQHLVLSVIYSLLVTLFIGILTLGIIAIIN
                     PMFLIIWPFILIFIFLLAVVSFAYLSPMQKARLRGIMIDTELPYVLRHISTELQAGIG
                     LYKTLQSVAKNDYGVLSQEMSRTIMEIENGTDTKTALRHMALRSQSKSFNIAIFHIVR
                     TLNTGGNLSLSIDSVADSVSFDLLEASKQFGEKMNFFGIIFIFIAIVLPVFLAILSSI
                     ANAPIGQGGELFLPGILTPTLLAIIYVIVMPAIFVFITYYIKMIEPKM"
     gene            8139..9044
                     /locus_tag="PLK55_02605"
     CDS             8139..9044
                     /locus_tag="PLK55_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ35850.1"
                     /translation="MAKLKEMMDRYQIYLYSQKLKIKASYWFAIVLIVSVLIGALAFL
                     INAKIGILLFAILLDLGFGIPYYLYFKNLDEIEKYWPEALRLIADTMKSGSSLDYALR
                     EASSADFGPLSKEFDEVIRRLEMGDTMAQSLDHMALRIESKIVRRTITLIQECLKTGA
                     QLADVLEEIANDTKQMFRIKKERVTKTMLQVIFIFAAGAVIAPFIFGLTNVITQFLTT
                     ISSTAGVATAGALATATATQESLAFLLDIYLLIEVAAAAVIVSLMRQGKFTNAVVFFP
                     IMVIISFIIYMVSQAVISGILIEAV"
     gene            9048..9227
                     /locus_tag="PLK55_02610"
     CDS             9048..9227
                     /locus_tag="PLK55_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HOZ35851.1"
                     /translation="MKIKELLKDRRGQVSLEVLLIVGIVVLAALLVGYYLKQTSMNNS
                     TTIFGYIRKIVSGTN"
     gene            9416..9853
                     /locus_tag="PLK55_02615"
     CDS             9416..9853
                     /locus_tag="PLK55_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HOZ35852.1"
                     /translation="MKFNKKGQGALEYLLLIGGAVLIAVIVIALLVGMGSSSNKDTAE
                     GGERISSAIAGTAIGSVLQSADCNSENYLNISYVHNGGDVNLMVNGDINTSRPVLDAG
                     KSTQAFNGEDVCSSANATYQISLFTTASSGATATSNTIIAKGE"
     gene            9934..10371
                     /locus_tag="PLK55_02620"
     CDS             9934..10371
                     /locus_tag="PLK55_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HOZ35853.1"
                     /translation="MKLKQKGQGALEYLILIGAAILVAVIVVSVLLGTSNSSKQTSED
                     TTGSYQELVTNTIIPPTIISVSCTDANATYIINESVTNGVTKYCVVIDGDVNISNCGA
                     PTNNKVIITGLSLPPDKYDMSLVAQKGNAYSAPTLPAPECTVE"
     gene            10397..10579
                     /locus_tag="PLK55_02625"
     CDS             10397..10579
                     /locus_tag="PLK55_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35854.1"
                     /translation="MFKKLLDDNKAQVSLEYLVIIVFGVMLVIITGIMIINLNILVSL
                     AKTKILNYRDNILTTI"
     gene            10599..11699
                     /locus_tag="PLK55_02630"
     CDS             10599..11699
                     /locus_tag="PLK55_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ35855.1"
                     /translation="MFYVTELILFFVFLFLVLASFSDIKTRTISNKLILFFLVFGISY
                     NITFALVSKNYSLLLTLLYSIIISLVFFLVLWELGVIAGGDTKLFIAISAIIPTMQGT
                     LGLFDIGIGKIEVLPIFSLILFVTSAFMVLPWILIYSKYLLFKKKHYKALINETFSRK
                     NLILLLDSIMVVVLVNLLISLLNITNIIFIFAISFVLTFLIFRIKIKLRFYYVILGLY
                     LIIFAILFSTHKLTIDLLITLLDVSVFVIFISILLIYLRYIKEKVLLETKAIAELKEG
                     DLPVYNYYYINKKIILKKPKFLNKIKEMALGTYYKGLKIDSSKACGLSREDITFLKDM
                     YNNRLVENTIYLKKTLSFTPAVLLAYMLLIIF"
     gene            11711..12172
                     /locus_tag="PLK55_02635"
     CDS             11711..12172
                     /locus_tag="PLK55_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35856.1"
                     /translation="MNRKGQLSIEVIVLLAIFLLFFQAMILPSIEFSEHVLQDTQAII
                     VSKKSVEDLAINIEQLASQDGYGKKQYYFYLPKNTILDCNVQAKILDYNITISKQQPI
                     PLGCNPEGFCDQFYRKLELQNFVICNKIGPGFTGVLILEKGADGNITIISE"
     gene            12177..12527
                     /locus_tag="PLK55_02640"
     CDS             12177..12527
                     /locus_tag="PLK55_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HOZ35857.1"
                     /translation="MNKKAQISLEFIIIIGFVLIITLFFANSVFGTTDINKALAKVKL
                     RTLDIFSMKGSIAQLEKIDYFVNDTNLQLNLYVNKNFDDFNLTLEDYSSTLENLENST
                     SFTDVNLAFIYINE"
     gene            12538..13131
                     /locus_tag="PLK55_02645"
     CDS             12538..13131
                     /locus_tag="PLK55_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35858.1"
                     /translation="MNSKYLLFQLFFIASIFVSLFIQKNLFLKVLLVCLLFLYGLLIL
                     PEFVYNRNKFLFIGSGFIILAILFLVVGYITNINYMIILSCVLVAFFYSSRVLFSTTY
                     GEVIITSPRNIQVRILDVFHRPGKIYTIETTKKYPLKSIVSIRLATSISRKPTEIIEL
                     LCDNSKTLLETSKIQKKPKKVIKTRKDTNISRKKKKK"
     gene            13143..13532
                     /locus_tag="PLK55_02650"
     CDS             13143..13532
                     /locus_tag="PLK55_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35859.1"
                     /translation="MFEKDLRAIIIFACVFGLLGILATMYFFEASAVSISSLLSKDAK
                     EGVYIVSGNLKSLDTKSNVLFFKLCDSFACISGVYFNPAKQIIDKLIKAKQTNTEITI
                     KARFEYYYDSPEIIAYKFYFNKLGDYS"
     gene            13529..14692
                     /locus_tag="PLK55_02655"
     CDS             13529..14692
                     /locus_tag="PLK55_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOZ35860.1"
                     /translation="MIFLEIVFGLLLGFVLGLIPSFHLNVLSYLLLLLGIFSLMYSSF
                     YFFFSLATSMTVAGIVPLLLFNAPTADNFTLNLGQQEDQSILPYIFGFVFGSLFSVLL
                     LPLFYLLFSFFSNFYIFIFLVLLVTLFYLVFSDSNRPLALMVVIFSGILGIITLKYNF
                     FFSEPLLPCIFGLFALPMLVVSFLDNTVDKSDSAPVQEIVQFKPAIFSAFLGSLFSSV
                     IAIVPSLSPGMAQIFPAVLFEKLSKHNRKVLLSSTLLSVILIYFFMAALFRKSRLGFV
                     SILLEKGAFPALSFPDILLLCFVFLFVVSLSCFILLVSFSNISKAIKSLPRKATIFCV
                     IVFSIWLIVLVTNIYSFFLIILSFCIGLLPIVYNKNKLLLMSYLILPTILFYI"
     gene            14702..15073
                     /locus_tag="PLK55_02660"
     CDS             14702..15073
                     /locus_tag="PLK55_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOZ35861.1"
                     /translation="MEKWKIKTKVLLSIMELAKASFPNEFSGMLVGDKDKKLIDDIYI
                     IPATHSSRNSSTIRLDLVPMSMSVIGSVHSHPSSNANPSRADLRLFSTKDINIIAHHP
                     YAANCFSAFNSKGQEISLEII"
     gene            15227..15748
                     /gene="grpE"
                     /locus_tag="PLK55_02665"
     CDS             15227..15748
                     /gene="grpE"
                     /locus_tag="PLK55_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOZ35862.1"
                     /translation="MNGDEKDLTIQEEIDLIKQKLIEQDKLSKEYLELLQRSQADFIN
                     YKNRIERETRDILFSETSKIFFEFLGYRETLIKAIDTEKDQGFKDSLNHLLISYENIL
                     KRFSLEKLEILKKPFDHNTSDCVLQQEVTDEKQNNLVISVIEDGYIQNKRLVKPAKVI
                     VGIFKNNKGDKNE"
     gene            15741..17555
                     /gene="dnaK"
                     /locus_tag="PLK55_02670"
     CDS             15741..17555
                     /gene="dnaK"
                     /locus_tag="PLK55_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOZ35863.1"
                     /translation="MSKVIGIDLGTTYSAVSVVEAGQAKILINSEGSRTTPSVVGLSN
                     GEIIVGALAKRQAVVNPDNTIYSIKRKMGTSEKVKIGGKDHSPEQISAYILQKLKKDA
                     EEQLGQKITQAVITVPAYFSDSQRKATKAAGEIAGLEVLRIINEPTAAALAYGIDKKH
                     AHTVLVFDFGGGTFDVSILELSDGVFEVKSTAGDNRLGGDDIDQKIIDYLSTEFKKKE
                     GIDLTKDKMAMQRLKDEAEKVKIELSTTLKATINIPYITATADGPKHLNLELTRAKLE
                     DMCSDIFKRLEIPTKQAIADSKLDISKIDEVILVGGSTRIPKVVDLVEKITGKKANKS
                     INPDEVVAMGAAIQGAVLQGEIKDVLLLDVTPLSLGIETLGGVFTKLLERNTTIPTKK
                     SQIFSTASDSQTAVDIHVLQGERPLAKDNLTLGRFQLVGIPPAPRGVPQIEVTFDIDA
                     NGIINVSAKDLGTGKVQTIKITSTTSLSKEEIERLKKEAEEHAEEDKKTAERIEVKHK
                     AESLIYSVDSVLEEAKEKLSPEDIEEIKKLKTDLQSAIDSDATDLEEKTTALQTKLYD
                     VSKKLYQQAPPQQEEPKSDGDKNDDGKTVDADYTTDDK"
     gene            17575..18717
                     /locus_tag="PLK55_02675"
     CDS             17575..18717
                     /locus_tag="PLK55_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOZ35864.1"
                     /translation="MAKDLYEILGVKKSSSQEELKLAYKQLVKKYHPDISKEPNAEEK
                     LKEITHAYSILSDENKRKQYDMVGESSYNGTGGFNYSNFSEDDFEFDLSDIFSRFGFS
                     REDIFGDFSGFRNMRGQGKRQEWAELDLKTRITIDFVTAVKGGKQEIKLDKDIVCVAC
                     DGTGSKSKARKTCENCGGRGRVMLRRQTPFGLFAIEQLCSKCQGHGTILSDPCKVCVG
                     KGYVKKTTDITLDIPAGVNNGDVVRLRGAGNVHEGKAGDLFIQVFVKEHDFFKRLGYD
                     LYCEIPIIYSDLVLGTKIKIKGIQDTINLTIPIGTRPETTFKVSGKGVPDPNRRGVHG
                     SLFVKVILAEPIDVNREYKQLLEKLASIDNKTKKKIQDKFKDYISF"
BASE COUNT         6548 a         2845 c         3098 g         6370 t
ORIGIN      
        1 caatcatatc atatattttt ttttctccgc cagcaagttt attggaataa ccaacaggat
       61 agattggcgg atggtccgtt gtttcttttt ctccgcgcga tgggtgtatt ggtttagata
      121 agatttcttt agcaaccgca gcatagtctt tgtcttgaag cacaatctcc actatttctt
      181 taagattaat gcttgcagga taaacggtgt tatctgttct cggatagctt attaaacctt
      241 gttgatataa gctttctgca atattcattg cttggttagt gcttattccg aataccgaag
      301 aagctgctct aagaaagcta gtagtgttaa aaggttctgg tctgttaagt gttttttctt
      361 ttttagagac ggctttaaca attccagtct tttcattttt tatattttca aatatttttt
      421 tgcctttctc aatatcaaag aactttccgt ctttgtggct tcctgaaaac ttaattttag
      481 ttttctcaaa atttgcaatt atttcaatat atttttctgg tacaaacttc tgtctttcaa
      541 gttctctttc tacaataaag tttagtaaag gcgtttgaac tcttccagca gatatgaaaa
      601 gttttccttt tctattagat actacagaaa gatatctagt aagtattgcg ccccataaaa
      661 gatcaacttc ttgacgcgca aacacagagt ctgcataatt aaaatctact tttcttaaat
      721 ttttaaaaga atcaattatc tctttttctg ttaaagcact aaaatacgct ctatgaagtt
      781 ttgtgttttt attattttca ataacatagt tgtaagcttc aacaccaatt gcttctcctt
      841 ctctgtccgc atctgttgca acaataatgt tttccaattt gtctcttttc tttttaaggc
      901 tagaaataat tcctttttca acttgaagat atgtaagttg tttatctgct aaaagatcaa
      961 gagtataaat attccagtag ttattgtctc caacaaagtc aacagtgttg atgtgccctc
     1021 taagcgggat aactagggta tccttgccat taaaattaaa agaaaagcag tttactccgt
     1081 tttccactat tgttgtgaaa ttgttgtttg agagaatgct tgcaatcctt tttcctgcaa
     1141 tcgctttctc tgaaattatt aagtccattt tattctagaa agtagtttgt aaaatctttc
     1201 ttggaagatt cttgctattt ttatgttctt ttaaaattgt cagaatgctt gcttttgtta
     1261 ttccgtcaat cttgaagatg ctgtcaagca aagtctttaa gtcgccttta tcaagagcca
     1321 tgactcttgc aagaacgttg tattctccag tgacttctac aacttcttta atgtcttcca
     1381 tttcttttag ttttttaata atgtcatttt gttttgaagg gtctattgta attaatattt
     1441 ctactttttc attaaagcca agtatctcat agtcaatctc tgcagtaatg gatttaatta
     1501 ttcctctttg cattaatcta tcaagtcttt tctttattgc gacatctgtt agattaatgt
     1561 cttttgcaat attagcaaag gatactcgcc catttgctgc taattgctct ataatgtatt
     1621 tgtcgatctt atctattact tgcatatttt atctcttttt aattatttaa atggctttaa
     1681 taaatcatat ttatcaagat atacttctgt ctttaatatt tcaataaaag gtataacaaa
     1741 cattacaaaa aaaaccattg caatgattgg tccagcgaat actccaaaga ctatatcaat
     1801 aatgtaagta aaaagtgtta atataaacgt tgttacaaac agataaacga agtatactgg
     1861 ctttttcttg atataattta agcttaaaaa aacagagtgt tctgcatttt gtttttccat
     1921 aataattatt tgtggtataa accaaaacaa tagagttatt ataaataacg cgatgttaac
     1981 aaacacatta ttaactaacc caaaatcaaa aagaatacaa agagcaacat ataataataa
     2041 ataaaaagaa atattgaatt ttaaaagctc aataaatggt ttttttattt ctctagagtt
     2101 tttattttca aaatcatatt ctcttcccat tttatatact acgatggttt gaagaagagt
     2161 atatgcaaag acaattgcaa taaaagccac aagcgttaaa ataataatta atggatcctg
     2221 gtttagcagt tcataagtta gatttagact tccccctgaa aggctaaaca aaggattaac
     2281 caaaaataat gtaaatatta ataaaagact agttgcaaaa acaagcttaa tattactaac
     2341 atatttttta aatgcaactg caatatccat actcataata attccccatt tatttttcta
     2401 attttttaat atatattact tagtttatat aatatattaa tagaattctt tttaaataat
     2461 taacctttac taaaaaacag gtctagcctc tagatatcct ggttttgcag cagttatttt
     2521 tacttttgaa gttttgccta agattaattt gtcttttggt acaactactt gcttatagtt
     2581 atttgttctg ccaattagtg tgttgttttt gccaagctct attaaaagta tttcttttgt
     2641 ttttccaata agtttcttat ttttttcaag agctattttt gcacaaattt tggtaacaag
     2701 tcttgatctt tcttttttta ttctgccatg taaatctttg taattgtttg cctctattcc
     2761 ttttctctgc ccaaacctag aaatatttac aatatcaaag ttacattttt tgagcacttc
     2821 aacagtttct agaaagtcct tttcagtttc tgttggaaag cctacaataa catctgttgc
     2881 gagtgttaag ccttttatgt tttttttaaa gtaattaatc aaatcaagat attctttctt
     2941 tgtatgatgt ctattcattt ttttaagaac cgcattgttt ccgctctgca ttggcacatg
     3001 taaaaattta tacatttttt ctgatttcat aatctgagag atttccgttt tataattttt
     3061 aaaatgttga gggtttccca tgcctaggcg tatcctgaag tctccaggaa ttgtaattat
     3121 ttctttaaga agagaagcta tgtttgtttt gatgtcaagg ccataacatc cagtatcttg
     3181 ggctgttaga agtatttctt ttgctccgtt gtttactgct tcttcaattt cggttttaat
     3241 atctttgctg cttttacttt tcaattttcc tcttgcaaac ttaactgcgc aataagcgca
     3301 attgccaaga caaccattag caataattag ggtctttgta atctcggctt ttgttttttt
     3361 tttaagattt ctaaatttat caagatattt tattaatttc gctttatctt ttattccaaa
     3421 cattttggct tttggcagaa gttcttttaa tttactaaaa tcaatatcta taagacaacc
     3481 agttagtatt agttctttat tagaaaattc tttttgatga tctacaatgt atttgtagat
     3541 tttggtttcg gtctgttctt tgactccgca agtatttatt attataatgt ctgcgctctt
     3601 ttcatctttg ctttctttaa ttcccaaaac aatgtcttgg ctgtctcttt ggtttaaagt
     3661 acagccaaag gttttaataa aaatagtttt catagtgcct ttattttttg tgtgcaatga
     3721 ttcctatagc cccagaaaca ataaaaccta agaatgaaga agacattagc tgttttaata
     3781 tctcgagatt tttaggtatt ttcatattat ttaaaattat ataaatttga taaacataaa
     3841 tataaacaat taaaacatag cacagaacac ttataacaat aaaaagaata aaaagcgtcc
     3901 acttattcca ttttgtgtat tcttcgacca tcggcattac aaaatacaat ccaaagaacg
     3961 cgacaactgg taaaaggata tacatcgggc tagtaaagaa gttttctttt gctaaaaaat
     4021 aaaataatcc ttgtgctaaa acataggaca ataagaagaa taaaataaat ttaagagttt
     4081 tgttcataat atcactatct ttaatataat atattaagta caaaagagtt ttaaatggtt
     4141 tagaatatta aaagaatata tttaaaaaga agttgataga ctattagaat agctaaataa
     4201 tatataatat agaatataat ggtataatta tgattacagc atctgcgcct tggacagaaa
     4261 agtacaggcc acaaaggctt gacgatattt ccgggcaaaa acacgtggta tctaagctta
     4321 agaaatatgt agaaaacaag tcaattccaa atatgctttt tgcaggtcca ccaggagttg
     4381 ggaaaacctg ttgtgctata gctctagcta aagaattatt taaagatggc tattctcaag
     4441 attttttaga gctaaatgca agcgatgaaa gagggattgg ggttgttaga ggggcgataa
     4501 aggattttgc caggaccatc gctctaactt caagttttaa aattatattt ttagatgaag
     4561 cagatgcttt gactcccgaa gcccagcagg ctctaagaag aaccatggag aaatacacaa
     4621 acaccgttag gtttattcta aatgcaaatt attcttctaa actaataccg ccaatacaaa
     4681 gcagatgtgc gttatttaag tttaaacctt taacaaaaga agatataaaa gacagaatag
     4741 aatatattgt taaagaagaa aaaataaaaa tagacaaaga agcaattgcc gcaattaacg
     4801 aagtttgcga aggagattta agaaccgcaa ttaatctttt acagactgcc gcttcaacta
     4861 gtgaacacat aactgaaaaa gatatttata ttgtttctaa ctctgctcag ccacaagaca
     4921 ttatcaattt aattgatgct tgtctaacaa aagatttttt aaaggcccga acaacactag
     4981 aaactttgtt gtttgatcac gggttgtcgg gagaagacat aatcaatcac atctataaaa
     5041 aaattattgc atatcctgaa aatcaaattt caatagataa aaaattaaaa ataatagaca
     5101 agattgcaga gtttaattat aggttagtgc aaggcgcaaa tgacagaata caactagaag
     5161 cattattgtc tcaaataata ttaatcgaat aggtgaaata aattggtatc atatctagat
     5221 gaactaaagg cccagatggc aaaagatatt gataaatcaa ataaaagtaa tgataaaaaa
     5281 atagaggcgc caccaaaaga aataaaaaaa gaagaactaa aaaccgcatc agcaaccact
     5341 acctcgaatc cagaaacaaa gccaaaagaa caaccaaagc tcgataatct taaagatgca
     5401 tttagttctg tctctcaaga attaaacaaa gtcgcaccaa agcaatctcc agaatcttta
     5461 aagtctttaa tgggagatat aaaagatgaa agagaagcag attctttaat ttctaaatgg
     5521 gaaagaacag gaaccgttat aacaaagtat ggcgacacag aagtctacag agttccggat
     5581 gaaccattat tttattataa aacaaatatt ccaaagccct cgcctagtca aaaactaatt
     5641 ataaatactt taaaagaagc aaccacaagg cttgtttcta cagaccctta taaaatcaga
     5701 gatccaaaac aaagacaaaa tatctattat caaaagattt tagaaattct agagaacgcc
     5761 ccagaaatta atgttccaaa gcatttgttt gacttctatg ctaaggcagt agttacagaa
     5821 atggtgggtt atggagtaat tgatacatta atacacgaca gtaagcttga agaaatcatg
     5881 attatcggcc caaaaaagcc agtatatatc tatcacaggg actatggaat gatgtataca
     5941 aatattattt tttattctga tgatgaaata caaaatatca ttgataaaat cgcaagaatt
     6001 cttggtagaa gagtagatat atctaatcct ttgcttgatg ctagacttcc ggatggctct
     6061 cgtgttaatg ccaccattcc tcctgcatca attgatggtt ctacaataac cattagaaag
     6121 tttagagagg atccatatac tatagtagac ctagttaact ttggaacaat caatttagaa
     6181 gttgctgcgt ttatgtgggt tgttgttgaa ggacttggcg caaagccagc aaacattcta
     6241 atatctggag gcacaggttc tggtaaaacc acgttactta atgttcttgc aaattttatt
     6301 ccagactcta gtagagttct aaccatcgaa gacacagctg aattaaaatt accaattaaa
     6361 cacattattc gttttgaagg aaaacctcct ggtttagaag gcacaggaga attgactctt
     6421 gaaattcttg taaaaaacac tttaagaatg agaccagaca gaataatagt tggtgaaatt
     6481 agacatttag aagcaagcac gctatttact gcaatgaata caggtcacga tggttgtatg
     6541 ggcacaatcc acgcaaattc tgcaagagaa actcttgtta gaattacgtc tccaccaatg
     6601 gatgttccta tgctaatgct tgcaggcctt gattttatta ttgttgaaaa aagaatgaat
     6661 acatcaagag ggctagtcag aagaatagtc gagattgcag agattacggg tgtttttgaa
     6721 aataatcctc aagcaaacat aatatttaga tggaatccac aaacagataa tcttgaaaga
     6781 acaaatgagc caataaagtt ctttgatatt attaaaatgt atacaaaata cggagattct
     6841 gatataaagc gaattttaga agaaaggata agccttataa atcaattagt taaaaataat
     6901 ataagaagca taaatgaagt ttcaaagact gtgcaagaag tatatactaa taatagtaaa
     6961 aaaaatgagt gatctaaaaa atgccagaag aagataaaag acttaaaatt ttaagaaaag
     7021 aattagataa agatgactct agtgcttcaa aagtagatct tgacgctata caaaatattg
     7081 tttcaaagat taaagacaaa gacagagcaa aaggcgtttc tggaaatgtt tctgaaagtc
     7141 acttaaaaga acttagagac ataatatctg aaggaaagct ggctaaactt aatgttgata
     7201 cagaagccaa acttttagaa aactctgcat cagaacttga taaaggcgca gtaaagatat
     7261 acaaggcagt tccgggtttt attaattata ttgtcaaatc tataataaaa agaagtatag
     7321 gaagaaaaat tgcattttat ttgtattctg cagatattaa aaaaactctt gtgcagcatc
     7381 tagttttatc agtaatttat tctttacttg taacattatt tataggaatt ttaactctag
     7441 gaataattgc aataattaat ccaatgtttt tgataatttg gccatttatt ctgatattta
     7501 tatttttact ggctgttgtt tcatttgctt atctttcgcc tatgcaaaaa gcaaggctta
     7561 gaggcataat gattgataca gaattgccct atgttctaag acacatctca acagaattac
     7621 aagcaggtat tggtttatat aaaactttac aatctgttgc aaagaacgat tatggtgttc
     7681 tttctcaaga gatgtcaaga acaatcatgg aaattgaaaa tggaacagac acaaaaacag
     7741 cattaaggca catggcttta agaagccaat ctaaaagttt taatattgca atttttcaca
     7801 tagttagaac attaaatact ggtggtaatc tttcattaag tattgatagt gttgcagatt
     7861 ctgtatcttt tgatttactt gaagcatcaa agcaatttgg agaaaaaatg aatttctttg
     7921 gtattatttt tatttttatt gcaattgttt tacctgtatt tttagcaata ttaagttcaa
     7981 ttgctaatgc tccaattgga caaggcggag agcttttcct tccaggaatc ctaacgccga
     8041 cattgcttgc aattatttat gtaattgtaa tgcctgcaat atttgtattt attacatatt
     8101 acataaagat gatagaacca aaaatgtgat ttaagaaaat ggcaaaatta aaagaaatga
     8161 tggacagata tcaaatttat ctatattcac aaaaactaaa gataaaagca tcatattggt
     8221 ttgcaatagt tttaattgtt tctgtattaa ttggcgctct tgcgttttta attaacgcaa
     8281 agattggaat tttattattt gctattcttc ttgatcttgg ttttggaatt ccatattatt
     8341 tgtattttaa aaatcttgat gagatagaaa aatattggcc cgaggcgcta agattaattg
     8401 cagataccat gaaatctggt tcttctcttg attatgcttt aagagaagct agttctgcag
     8461 actttggccc cctttcaaaa gagtttgatg aagttattag aagacttgaa atgggagata
     8521 ccatggcgca gtctttagat cacatggctc ttagaatcga gtctaaaatt gttagaagaa
     8581 caattacttt aattcaagaa tgcctaaaga ctggtgcaca gcttgcagat gttcttgaag
     8641 agattgcaaa tgacacaaaa cagatgttta gaattaaaaa agaaagagta acaaaaacca
     8701 tgctgcaagt aatttttata tttgctgcgg gcgcagtcat tgctcctttt atttttggtt
     8761 tgacaaacgt tattactcag tttttaacaa ctatatcttc aacagctggc gttgcaacag
     8821 caggcgcctt agcaaccgcg actgccactc aagaatcttt agcgttttta ctggatattt
     8881 atttattgat tgaagttgcc gcagcagcag ttattgttag tttgatgagg caaggcaagt
     8941 ttacaaatgc cgttgttttc tttccaatta tggttattat ttcttttata atatatatgg
     9001 tatcacaagc ggtgatatct ggaatactaa tagaggcagt ataaactatg aaaattaaag
     9061 aactattaaa agacagaaga gggcaagtaa gtctagaggt gttattgatt gtgggcattg
     9121 tagttttagc tgcgcttctt gttggttatt atcttaagca gacctctatg aataacagta
     9181 caacgatatt tgggtatatt agaaagatag tttcaggtac aaactagcct cttttaaagc
     9241 caataaataa gccacaaagc atagtgtttt ggtttggcta actaaacaga aaggtatata
     9301 aacataatct ataacaataa taaataatta ttttattaaa taaaataaat aaaatatatt
     9361 taagaaatta aatatatcca aaaaataaaa atgaaaatta aacggaggaa taaaaatgaa
     9421 gtttaataaa aaaggacaag gtgcacttga ataccttttg ttaatcggtg gtgctgtatt
     9481 aattgcagtt attgtcatcg cattacttgt aggtatgggt tcatccagta ataaagatac
     9541 tgcagaaggc ggagaaagga taagcagtgc aattgcagga acagcaattg gttcggttct
     9601 tcaatccgca gactgtaatt ctgaaaatta tctaaatata agttatgtgc ataatggggg
     9661 agacgtaaac cttatggtaa atggagacat aaacacatca cggccagttc tagatgccgg
     9721 gaagtctaca caagcattta atggagaaga tgtctgtagt tccgcaaatg caacctatca
     9781 gatttcgtta tttacaactg catcatccgg agcaacagca acatccaaca ccattattgc
     9841 taaaggagaa taaattttta tcctgtttta aacaggattt tttttatttt taaagggata
     9901 caactaaaaa aataaactta tttaatgata aaaatgaaac taaaacaaaa aggccaaggg
     9961 gcattggaat atttaattct tattggtgca gctatacttg tggctgttat tgttgttagt
    10021 gtgcttcttg gtacaagcaa tagtagcaaa caaactagtg aagacactac aggatcttat
    10081 caagaactag taacaaatac cataataccg ccaacaatta ttagcgtttc ttgtactgat
    10141 gctaatgcaa catatattat taacgaatca gtaacaaatg gcgtaacaaa atattgtgta
    10201 gtaatagatg gagacgtaaa tataagtaac tgtggggcgc caaccaacaa taaagtaatt
    10261 atcacaggct tatcgctacc accagataaa tacgatatgt ctttagtggc tcagaagggt
    10321 aacgcatatt ctgcaccaac acttcccgcg ccagagtgta ctgtcgaatg atgcgacaaa
    10381 taaaagaaga aaaaatatgt ttaaaaaatt actagatgat aacaaagcac aagtttcttt
    10441 agagtatcta gtaataattg tctttggagt aatgctagta attattacag gaattatgat
    10501 aataaatcta aacattctag tatctcttgc taaaacaaaa atactaaact acagagacaa
    10561 tatcttaacc actatataaa tctataaaat aagataacat gttttatgta acagaattaa
    10621 tacttttttt tgtgttctta tttttagtgc ttgcttcttt ttctgatatt aaaactagaa
    10681 ctatttcaaa taaacttatt ttgttttttc tagtttttgg aatatcttat aatattactt
    10741 ttgctttagt ttctaaaaac tatagtttat tattaacatt attatattca ataattatct
    10801 ctttagtgtt ttttctagtt ctctgggaac ttggtgtgat tgcaggagga gacacaaagc
    10861 tatttattgc tatatctgca ataatcccta caatgcaagg cactcttggt ttgtttgata
    10921 ttggcatagg aaaaatagaa gtcttaccta tattttcttt aatcctattt gtaacaagtg
    10981 cctttatggt tctaccatgg attcttatat attctaaata tctgttattt aagaaaaaac
    11041 actataaagc tcttataaat gagacttttt caagaaaaaa cttaatatta cttttggatt
    11101 caataatggt ggtggtttta gttaatctgt taatatcatt attaaatatc acaaatatta
    11161 tttttatttt tgctatttct tttgttctta cctttttaat atttagaata aagataaaac
    11221 ttagatttta ttatgtgatt ttaggactat atctaattat ttttgcgata ttgttttcaa
    11281 cacacaaatt aacaatcgat ttgcttatca ctttactaga tgtttctgtc tttgttattt
    11341 ttatttcaat attattgatt tacctaagat acataaaaga aaaagtgctg cttgagacaa
    11401 aggcaatagc agaactaaaa gaaggggatc tcccggtata taactactat tatattaata
    11461 aaaagataat actaaaaaag ccaaaattct taaacaaaat aaaagaaatg gctttaggga
    11521 cttattataa gggcttaaag atcgattcta gtaaagcttg cggattatct agagaagaca
    11581 taacattttt aaaagatatg tacaacaata gattagtgga gaatacaata tatttaaaga
    11641 agacgctatc atttactcca gcagtccttc ttgcatatat gttgttaata atattttaaa
    11701 agtgatattt atgaatagaa aaggacagct ctcgatagaa gtaatagtac tactagcaat
    11761 atttttattg ttctttcaag caatgattct gccatctatc gagttttcag aacacgtctt
    11821 acaagacacg caggcgatta tagtttcaaa aaaaagcgtt gaagacttgg caattaatat
    11881 tgagcaactg gcttctcaag acggatatgg caaaaagcaa tattactttt atttgccaaa
    11941 aaacacaatt ttagattgta atgttcaagc aaagattcta gattataata taacaatatc
    12001 aaaacagcag ccaattccgc ttggttgtaa tccagaagga ttttgcgacc aattctatag
    12061 aaaactagaa ctgcaaaatt ttgtgatttg taataaaatt ggtcctggat ttacaggcgt
    12121 attaatttta gaaaaaggcg cagatggtaa catcacaatt ataagtgaat aaaaaaatga
    12181 ataaaaaagc acagattagt ttggagttta taataattat aggttttgta cttataatta
    12241 cattgttttt tgcaaattcg gtttttggca caacagacat taacaaagca cttgcaaagg
    12301 ttaaactcag aaccctagat atcttttcta tgaaaggttc aattgcacag cttgagaaga
    12361 tagattattt tgttaatgat acaaatctac aactgaacct ttatgtcaat aaaaactttg
    12421 atgactttaa tcttacttta gaagattata gctctacatt agaaaattta gaaaactcga
    12481 caagttttac agacgtaaat cttgcattta tttatattaa cgaataaaga gataaacatg
    12541 aatagtaaat atctattatt tcaactattt ttcatagcca gcatattcgt aagcttattc
    12601 attcaaaaga atcttttcct taaggttctt ttagtgtgtt tgcttttctt atatggctta
    12661 ttgattcttc cagagtttgt atataacaga aataaatttt tatttattgg ttctggtttt
    12721 attatacttg caattctttt tttggtagtc gggtatatca caaatattaa ttacatgatt
    12781 atcctatctt gcgtattagt tgcgttcttc tattcttcaa gagtgttgtt tagtacaact
    12841 tatggagaag taataataac ctctcctaga aacatacaag tcagaatact agatgtgttt
    12901 cacaggccag gaaagattta tactatcgag actacaaaaa aatatccttt aaaatcaata
    12961 gtttcgataa ggcttgccac ctctatttct agaaagccaa cagaaattat agagcttctt
    13021 tgtgataact ctaaaacact tttagaaact tcaaaaatac aaaaaaaacc aaagaaagtt
    13081 ataaaaacca gaaaagatac taacatttct agaaagaaaa agaaaaaata agacttgtag
    13141 aaatgtttga aaaagattta cgcgccataa ttatctttgc gtgtgttttt gggctgcttg
    13201 gaattctggc aactatgtat ttttttgaag cttcggcggt ttcaatttct tctcttctat
    13261 ctaaagacgc aaaagaaggt gtttatatag tttctgggaa tctaaaatct ttagacacaa
    13321 aatcaaatgt tcttttcttt aagctatgtg attcttttgc ttgtatttct ggggtttatt
    13381 ttaatcctgc aaagcaaata atagataaat taattaaagc aaagcaaaca aacacagaaa
    13441 ttacaataaa agcaagattt gaatattatt atgattctcc agagatcatc gcctacaagt
    13501 tttattttaa taagcttggc gattattcat gattttcttg gagattgttt ttgggttgct
    13561 tcttggcttt gtcttgggat tgattccaag ctttcatctt aatgttcttt cttatctttt
    13621 gcttttactt ggaatttttt ctttaatgta ttcaagtttt tattttttct tctctcttgc
    13681 cacctctatg acggttgcag gcattgtacc cttacttcta tttaacgcac ccacggcaga
    13741 caactttaca ctaaatctcg gacagcaaga agaccaaagc atccttcctt atatatttgg
    13801 atttgttttt ggttcgttgt tttcggttct tcttttgcca cttttttatc ttctcttttc
    13861 ttttttctct aatttttata tttttatttt tttggttttg ttagtaactc ttttctatct
    13921 tgttttttcg gactccaata ggccattagc gcttatggtt gttatctttt ctggtattct
    13981 gggcatcatc actttaaaat acaatttttt cttttccgag ccgcttttgc catgcatctt
    14041 tggcttgttt gcgttaccga tgttagtcgt ttcttttctt gataatacag tagacaaaag
    14101 cgactctgcg cctgtccaag aaatagtgca atttaaaccg gcgatttttt ctgcatttct
    14161 gggttctctc ttttcttcag taatagcaat agtgccctct ctaagtccag gcatggcaca
    14221 gatttttccg gcagtactct ttgagaagct ctctaaacat aatagaaaag ttcttctctc
    14281 aagcacacta ctctctgtta ttttaatcta tttctttatg gctgcgcttt ttagaaaatc
    14341 aaggcttggc tttgtttcta ttcttctaga gaagggggca tttccagcac tttcttttcc
    14401 agacatcctt cttctttgtt ttgtttttct ttttgtagtt tcgctttctt gttttatttt
    14461 gcttgtttct ttttctaaca tttcaaaggc aattaaaagt cttccaagaa aagcaacaat
    14521 cttttgcgtt attgtttttt ctatttggtt aatagttctt gtaacaaata tatattcttt
    14581 ttttttaata attttgtctt tttgtatagg tctgttgcca atagtttata ataaaaataa
    14641 attgcttctt atgtcttatt taatattacc cacaatttta ttttatattt aggtgaaaaa
    14701 tatggaaaaa tggaaaataa aaaccaaagt gcttttatca atcatggaac tagcaaaagc
    14761 gtcttttcca aatgagttta gtggaatgct tgttggcgat aaggacaaaa aactaattga
    14821 tgacatctat attattccag caactcacag tagcagaaat tcatcaacta tacgtcttga
    14881 tcttgtgcca atgtctatgt ctgtgatagg ctcagttcat tcccaccctt caagtaatgc
    14941 caatccttca agagcggatc taagattatt ttccacaaaa gacataaata tcattgccca
    15001 ccatccttat gcggcaaatt gcttttctgc ctttaatagt aaaggtcaag aaatctctct
    15061 tgagattatc taattttccc tcaaaatagg cccaaaaaaa caaaaattta taaagcaatt
    15121 ggcaaaaagc atttattact tttgtagcat taatctattt agagcaaagg gaatagtaat
    15181 tttctcttgc gcgaattact aaaaattaaa aaattaaggt atatctatga atggagatga
    15241 aaaagattta acaatccaag aagaaattga cttgattaag caaaaactta tagagcaaga
    15301 taagctttca aaggagtatc tggaacttct tcaaagatct caagcggatt ttattaatta
    15361 taagaacaga attgaaagag agactagaga tattcttttc tctgaaacca gcaaaatatt
    15421 ttttgagttt ttaggctaca gagaaacact aatcaaagca atagatacag aaaaagacca
    15481 aggtttcaaa gattctctta atcatctatt aataagttat gaaaacattc taaaaaggtt
    15541 tagtcttgaa aagcttgaaa ttctaaaaaa gccttttgat cataacacaa gcgattgtgt
    15601 tcttcaacaa gaagtaacag atgagaagca aaacaatcta gtaatttctg taatagaaga
    15661 tggctacatt caaaacaaaa gattagtaaa accagcaaaa gtaattgttg gaatatttaa
    15721 aaataataaa ggtgataaaa atgagtaaag taataggtat tgatttaggc acaacatatt
    15781 ctgcagtttc agtagttgaa gcaggacaag caaagattct aattaatagc gagggctcaa
    15841 gaacaacgcc ttcagttgtt ggtttaagta atggtgaaat aattgttggg gcgttagcaa
    15901 aaaggcaagc agtagttaat ccagataaca ccatatattc cataaaaaga aaaatgggaa
    15961 caagcgaaaa agttaagatt gggggtaaag atcattctcc agaacagatt tctgcttaca
    16021 ttctacagaa actaaaaaag gacgctgagg agcaacttgg tcaaaagata acacaagcag
    16081 taattacagt tcctgcatat ttttcagact ctcaaagaaa agcaacaaag gctgcaggag
    16141 agattgctgg tctagaggtt ttaagaataa ttaatgaacc aacagctgcg gctttagctt
    16201 atggtataga taaaaagcac gctcacacag ttcttgtttt tgattttggt ggcggaactt
    16261 ttgatgtttc aattctagag ttatctgatg gagtctttga agttaagtct actgctggcg
    16321 ataaccgtct aggtggtgac gacattgacc aaaagattat tgattatctt tcaacagagt
    16381 tcaagaaaaa agaaggaatt gatctaacaa aagacaagat ggcaatgcag agattaaagg
    16441 acgaagcaga gaaagttaaa attgagttgt ctacaactct taaagcaaca ataaatattc
    16501 cttatattac agcaacagcc gatggtccaa agcatttaaa tctagaatta acaagagcaa
    16561 agctagagga tatgtgttct gatattttta aaagattaga gattccaaca aagcaagcaa
    16621 ttgctgattc aaagctagac atctctaaaa ttgatgaagt aattctagtc ggcggttcaa
    16681 caagaattcc aaaggttgtt gatttggttg aaaaaattac agggaagaaa gcaaataagt
    16741 ccattaaccc tgacgaagtt gtagctatgg gtgctgcaat tcaaggtgct gttcttcaag
    16801 gagagattaa ggatgtttta ttacttgatg ttacgccatt gtctcttggt atagaaaccc
    16861 tgggcggagt ttttacaaag ctattagaaa gaaacacaac aatcccaaca aagaagtctc
    16921 agattttttc aacagcttct gattcccaaa cagcagttga tattcatgtg cttcaaggtg
    16981 aaaggccttt agctaaggac aatctaacac ttggaagatt tcaattagtt ggtattcccc
    17041 cagcaccaag aggtgttcct caaattgaag taacttttga tattgatgct aacggcataa
    17101 taaatgtttc tgcaaaagac ttaggtacag gaaaagttca aacaattaaa attacttcca
    17161 caacatctct aagtaaagaa gaaatcgaga gattaaaaaa agaagctgaa gagcacgccg
    17221 aggaagataa aaagacagca gaaagaatag aagtaaagca caaagcagag tctttaatct
    17281 attctgtgga ttctgttctt gaggaagcaa aggaaaagtt atctcctgag gatatcgagg
    17341 aaatcaaaaa actaaaaaca gatcttcagt cagcaattga tagtgatgca acagatcttg
    17401 aagagaaaac aactgctctt caaacaaagc tttatgatgt ttctaaaaag ctttatcaac
    17461 aagcaccacc acaacaagag gaaccaaaat ctgatggcga taaaaacgat gatggtaaaa
    17521 cagtagatgc agattataca actgatgaca agtaaaccaa tgagttgatg aattttggca
    17581 aaagaccttt atgaaatatt aggagttaag aaaagttctt ctcaagagga actaaagctt
    17641 gcatataagc agcttgtaaa aaaatatcat cctgatattt ctaaagagcc aaatgctgag
    17701 gagaaattaa aagaaataac tcatgcttat tctattctaa gtgatgaaaa taaaagaaag
    17761 caatatgata tggttggtga gagctcatac aacggcacag gcggttttaa ttattctaat
    17821 ttttcagaag atgattttga gtttgatctt tctgatattt tttctaggtt tggtttttca
    17881 agagaagata tctttggaga tttctctggc tttagaaaca tgagagggca aggcaaaaga
    17941 caagaatggg cagaactaga tttaaagaca agaattacta ttgattttgt tacagcagtt
    18001 aaaggcggta agcaggaaat aaagttagat aaggatattg tgtgtgttgc ttgtgatggc
    18061 actggttcaa agtcaaaagc aagaaaaact tgtgaaaact gtggcggcag aggccgggtt
    18121 atgctaagac gccagacgcc ttttggtctt tttgcaattg agcagctttg ttctaagtgt
    18181 caaggtcacg gaacaatcct ttctgatcct tgtaaggttt gtgttggcaa aggctacgta
    18241 aaaaaaacaa cagacattac tttggatatt cctgcaggcg ttaacaatgg cgatgttgta
    18301 agactaagag gtgcaggaaa tgttcatgag ggaaaagcag gcgatttatt tattcaagtg
    18361 tttgttaagg agcatgattt ttttaaaaga cttggttatg atttatattg cgagattcca
    18421 ataatctata gtgatttagt tcttggaaca aagattaaga ttaaaggaat tcaagataca
    18481 attaatttaa caattccaat aggcacaaga ccagaaacta cttttaaagt ttcaggaaaa
    18541 ggcgttcctg atccaaacag aagaggtgtt catggttctt tgtttgttaa agtaatcctt
    18601 gcagaaccaa ttgacgttaa cagggaatac aagcagcttc tagagaagct tgcaagtatt
    18661 gacaacaaaa caaaaaagaa aatccaagat aaatttaaag attatatttc tttctagaaa
    18721 tattcagata acgatttctg cattcttgct tctttgtctg agaaaagaga gttaatttcg
    18781 tcttcactaa tatttagtct ttgaagaaga tgtggattta gctcatagtc tattactagg
    18841 ttctttgcta cttcaagata t
//
LOCUS       DAOOON010000014        18192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_3738_length_18192_cov_20.976733, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000014 DAOOON010000000
VERSION     DAOOON010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 18192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18192
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_18192_cov_20.976733"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..813
                     /locus_tag="PLK55_02680"
     CDS             88..813
                     /locus_tag="PLK55_02680"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:NF003075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HOZ35865.1"
                     /translation="MIPNSLGAAAAGYDRSTTMFSPEGHLYQVDYASKIIEKGSPAVA
                     IIYKQGILIIADKKIHSSLVIPDSVEKVFKIDNHIWTTCAGLIGDGRRLVDFARQMSQ
                     ENRTYYDQAIEVETLVKKIASVIQYYTQYGGGRPFGVSLIIAGYDSLGKHIFEAEPSG
                     AITAYKALAIGQNRQKLMDYLEKSYKENLSFEDALTIGIKAISLNLEPKQKLNVQGIE
                     VINVTDNGNTDAVVPLEKYSKLL"
     gene            824..>1260
                     /locus_tag="PLK55_02685"
     CDS             824..>1260
                     /locus_tag="PLK55_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HOZ35866.1"
                     /translation="MVKLDDAVLAKYSRDGHHFEILVDPYLAWDFKHGKADVNFDNIF
                     AMEEVYADSAKGKLASEEIVKQVFQTSDFLTIAKKIILDGDVQLTTAQRNEMLKRREN
                     EIIDFIAKNAHDPKEMTPIPPQRIINALEAGKFKFNLSRKKED"
     assembly_gap    1261..1360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1407..2015
                     /locus_tag="PLK55_02690"
     CDS             1407..2015
                     /locus_tag="PLK55_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOZ35867.1"
                     /translation="MKRIVIPGELIADQRKKVGENVFIKENKIYSSVLGLLNETDDFV
                     SIVPLNGPYIPQENDPVVCVVRNVTANGYIIDVNSHSDCFFPKSMITRELKIGQMLFA
                     RVKGVDGVEVSELDNINILPDGLIYSVSSVKIPRIIGKNDSMLNILKKYTSSNILVGR
                     NGWIWYVSKNGNLLEKALSLIVTNSQKSNLTNTIEEYLKNNQ"
     gene            2032..2739
                     /locus_tag="PLK55_02695"
     CDS             2032..2739
                     /locus_tag="PLK55_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964829.1"
                     /note="involved in the 3' to 5' degradation of a variety
                     of RNA species; forms a trimer of heterodimers (hexamer)
                     with Rrp42; Rrp41 is the catalytically active subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HOZ35868.1"
                     /translation="MSNVKRPDGRSYDELRPIKVELGTAKNAEGSCYLEWGKNIVIAT
                     VNGPKPVLPKHLANPSKAIINYKYRMATFSVSERKSPVPGKRDKEISLVSGHALEAAV
                     MVDQFPNTMIDVNVLILSADAGTRVAALTAASLAAADAGLPMRGIISAVAAGRANKQI
                     VLDLDKEDEDTEDAVDMACAFLMPQKELALLQMDGFVDKKDCSKILETALKGAEKVYE
                     IQKKALLEKYPVEGEIQ"
     gene            2736..3545
                     /locus_tag="PLK55_02700"
     CDS             2736..3545
                     /locus_tag="PLK55_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35869.1"
                     /translation="MSDIDILKIIKADKAKKLLQEEKRFDNRGLLDSRNIEIKRNVMK
                     SADGSAWVKLGNTEIIAGVKFAVGTPYVDSPEEGSLVLNLEMSGIASADIDTGPPSID
                     AMEYGRIVDRVIRSSECVDFKDLNIVTGEKSFTVYVDCYALNADGNLIDAAQYAAMAA
                     LLDCKIPKLDENNNIVNKEYSDKKLNIDTKKIAASFTFWKTENKIFADATEVEDLASD
                     TRFTLGVVGSNVVAYHKGGGNGTLKIEDINVMMDIASKKYLEIKNKICEEQ"
     gene            3542..3766
                     /locus_tag="PLK55_02705"
     CDS             3542..3766
                     /locus_tag="PLK55_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ35870.1"
                     /translation="MSTKKVGKTGKYGVRGGVGIRKKYLLAIDDKSKKVCPNCGTKDK
                     LKRVATGIYQCQKCKTKVSGGAYKFKTTGQ"
     gene            3775..3903
                     /locus_tag="PLK55_02710"
     CDS             3775..3903
                     /locus_tag="PLK55_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35871.1"
                     /translation="MYYCGKCNEVYNEMPESKKCKSCSGRIFYKKRAPIVKKVKAD"
     gene            3908..>4099
                     /locus_tag="PLK55_02715"
     CDS             3908..>4099
                     /locus_tag="PLK55_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35872.1"
                     /translation="MLITTSRNADISQKRFCKYLSVFFPEVKHIPRGQSQLGKLFNKA
                     NYLGDDFLLIVGKKKENLEL"
     assembly_gap    4100..4199
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4200..4500
                     /locus_tag="PLK55_02720"
     CDS             <4200..4500
                     /locus_tag="PLK55_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35873.1"
                     /translation="LGDDFLLIVGKKKENLELMVYKRKQKEFFPDKSFLLTDVFYKGP
                     KDKIQSTNAKGKFFYFLEKTDKDSEIKASQKESEVVFKIKKEILFSFKILKEEKA"
     gene            4497..4748
                     /locus_tag="PLK55_02725"
     CDS             4497..4748
                     /locus_tag="PLK55_02725"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOZ35874.1"
                     /translation="MIDYIIKEELIINTRNPKKLKELLDIEMSRKDFKRSESKITIDN
                     KTKTLKIIILAKDITAYKATINNYINLLELIKKTYEVDL"
     gene            4745..5080
                     /locus_tag="PLK55_02730"
     CDS             4745..5080
                     /locus_tag="PLK55_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016272 - prefoldin complex [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit"
                     /protein_id="HOZ35875.1"
                     /translation="MSENVNMYRQQLMFLTQQKQQLQFQMNVLENTLKELEKTKEKKV
                     YKGVGNVFILSDKEDVLKDTKDLKETMDLRIKNIQKQEDVILKKINEKTKAESKNKSE
                     DKEQMNGVQ"
     gene            5170..5745
                     /locus_tag="PLK55_02735"
     CDS             5170..5745
                     /locus_tag="PLK55_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35876.1"
                     /translation="MAKKENKSLIIWAVICLIVGLVMGLFLTTVVTTGKAMQALSEKS
                     TANLEEARTNLVLNLLKVNTIEKNGNGQLNILSQGQNGQILMEGNTGVTLGTNGTSFI
                     MGGTPQENGISMASQNVNINTNAFRVSAHGETENALAIYPLGDSEGSKRITISVPLKY
                     NVAMDVNYGADNYYACLTDEGTLFKSYSPCN"
     gene            complement(5772..6188)
                     /locus_tag="PLK55_02740"
     CDS             complement(5772..6188)
                     /locus_tag="PLK55_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1699 family protein"
                     /protein_id="HOZ35877.1"
                     /translation="MYTIKDTLSDFLKISQLNSNCEELHVTRAFSKKNLTKILAKCKR
                     LKTITISNSTNERISKALKEFIKKKNITIILRKEQGRPIDIKVEKLQKILREYKDYSY
                     RDLEKKLGIPKSKIHYLIKYSKRRKIKDGNKIIYLK"
     gene            6350..7243
                     /locus_tag="PLK55_02745"
     CDS             6350..7243
                     /locus_tag="PLK55_02745"
                     /inference="COORDINATES: protein motif:HMM:NF033579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOZ35878.1"
                     /translation="MLNILQPISFNTFFKQVERVFGEVRLPFHFSKYSNKLYNNFVHV
                     YLLILKERHRCSYRRLTELLNDINVLRMLGIQKLPHFTTLQKFVSRISKDVFQKLVMA
                     CHKLINSKDLIVAVDSTGFVNVNPTYYYLNRCGIRPPYKNFTQTSIVVDTNTKLVLNI
                     CTNSHRKNDTQSFIPLLDKLKHNIVRALADKGYDSRELREYCWNNNITVHIPFRKLPC
                     YQTPSDNRIKAMKRFRTKSYNKRSVVESVISAIKRTTGGFVTATKQDNQQKQVILKVL
                     TYNLEKLSRNQKIISFTILIC"
     gene            7302..9041
                     /locus_tag="PLK55_02750"
     CDS             7302..9041
                     /locus_tag="PLK55_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ35879.1"
                     /translation="MIQSFLLSFGIIILFSTLMLYIFTKLKQPPLLAFILTGILIGPM
                     VLGLVTNTEEIALLVEIGIAFLLFSVGLGTDLDELKKINLGLIILPIANIIITLLVFL
                     IFKNLLAIDFIQALYFSFIVSFSSTMLIAKILLDNFKMDSLDGRLTIGVLLVEDLIAI
                     LAIPLLKNISNLTVGLVGIIFVKAALLVVIAIILNKTIYPYIIKNAYKSDQSFFMLSI
                     ASCFLFILISVILDFPMAIGAFFGGLAISIYPYNYEISSKISGIRNLLTTIVFVSLGM
                     KLSFNFSDNLLLLIILLILVLVIKPIIHFLFVLFSGYGSKIALGVSTYKAQISEFSLI
                     LAIQGFSLLQLTKGQYSAVIIATSLSMLLTPYIINSQQKLYLKFIKSFSNLDKNKKFT
                     KEIDSLNSVSKDIENHLVIVGADVTSDAIIKLLGKETNNPIVLVDLNPDKISGINHNR
                     ISGICGDINYPDVFNAANIKSAKLAIITVSEFDTTARFIKNVKKVNADLPIFARAHTR
                     KEAYKLYELGAELVIIPKVLESNYLLEKTYDFMLNQNTKSTALRSKYLEYLKKEAAKN
                     KETTRKKESKFGF"
     gene            complement(9024..10097)
                     /locus_tag="PLK55_02755"
     CDS             complement(9024..10097)
                     /locus_tag="PLK55_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOZ35880.1"
                     /translation="MTKSYKIVYAINADGFGHVTRSIPIIDHLLHKKHNIEIITDKKV
                     ADFLQHKYKQLKIHIVPQVYFEYTNDSVKAFKSFFKCFNKHNFNNLKKATLLIKKIKP
                     DIVISDLERYSLWGGKFLELPTIEIDNHATIFRGDVEFKQKDAADFLQAKSVSKFVYP
                     FANFYFGLCFYRTQITKKNTEVFDPIFLDDIKKYKAKDGDYILVYNRFMDKAKMVPTL
                     KKIKNQKFVIFGYNVNKIDENLEFRKAKPNLFKEIAESKAIITNGGHTLITEAIYFKK
                     PIYSIPIKHQFEQLTNAYYLEKLEYGLSTKKIDAINLRRFIKNIPYYKTKLEKHKYQD
                     KKYFLKRLDEKINELITESKTKL"
     gene            10151..10738
                     /locus_tag="PLK55_02760"
     CDS             10151..10738
                     /locus_tag="PLK55_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOZ35881.1"
                     /translation="MIVLICGFSASGKSYLVNSVAKKLKYKHIHTSDILNQFASGIPE
                     NKIQIENTKMNNGWYEFSGLDKKRDKSKFLDKKLDKFLIKLVNTKDNLVLDSWTLPYL
                     VKKNPKIIKIWLDATEIERAKRLSLRDKISFKEAHTVLKEKDSFSKTHFQKLYGFTLG
                     KDKKVFDLVINTNKLSIKQVEEKTMSFLKKKIKIK"
     gene            complement(10731..11558)
                     /locus_tag="PLK55_02765"
     CDS             complement(10731..11558)
                     /locus_tag="PLK55_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOZ35882.1"
                     /translation="MKCDLHTHTYFSDGFHSPEYLIEKAISKKLKALAITDHNSIAAL
                     PIARRIAERENIELITGVEINCESTEILGYFFDTTYEPLLKIMEENAKSINDVTMQKF
                     YWLKDNGYSIDLNDMMQFVGPNKLLQATHLALELKKKGYVPTLQDAFSIIIKKIKIDY
                     KPKRYSTKKVISEIINANGVAVLPHPWWLPGATKNDIEAYLSKLKEQGLAGIETTGPH
                     PKEDEKLITKIKEIVKAKGLIETGGSDFHSLSHFPENKLGEYTVNYSVIEKLRKRII"
     gene            complement(11575..11784)
                     /locus_tag="PLK55_02770"
     CDS             complement(11575..11784)
                     /locus_tag="PLK55_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HOZ35883.1"
                     /translation="MTDLPVAAIDRLIRKAGAERVSEDAAEELSKILSEVAMDISKQA
                     IELAKHAKRKTITGEDIKLATKTMQ"
     gene            11895..12767
                     /locus_tag="PLK55_02775"
     CDS             11895..12767
                     /locus_tag="PLK55_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35884.1"
                     /translation="MTLMDSILLQINQKKTSFNELLTRIIPNYSSTESARAALSRVIK
                     NLVSFGYIQKTEDFFEITEKGKKIVESKLQNKMLNNINSINDLLQKSQPAKSLQYLDD
                     IVRNLQIFLERSKEDTTLLKIGKTSATFYISDLEDIKKNVETRLINFEQSLDRQIMAL
                     KDLNFEDQFIINLDENAFSLIKELSENNNISELVIDCDKTDINSGMLFETHSEFTKKP
                     DNVFVLKLSDIAILKKLLLSRLDIIMQTRFKVYIKEIVIYLKIGKLYFTGPYNQILAI
                     KENYQKKTKSQEKK"
     gene            12768..13430
                     /locus_tag="PLK55_02780"
     CDS             12768..13430
                     /locus_tag="PLK55_02780"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOZ35885.1"
                     /translation="MKIEYIGHSCFVISYQNHNILIDPFISKNPMLKANLENFTPDLI
                     LVSHDHFDHVGDTESIAKANNSTVVCENALGEKLKEKGLWVLSGTIGDTINYKGIEVT
                     FVKAIHRSDNGIATGLIIKTEEHTIYFAGDTDYFIEIKEIAENFKPDIAILPIGGVYT
                     IDPKGAIEFLKELKPKFVIPMHYKTFMNLYGTPEQLQELIEKENLDIKQITLDFGQSI
                     EI"
     gene            13440..13510
                     /locus_tag="PLK55_02785"
     tRNA            13440..13510
                     /locus_tag="PLK55_02785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13472..13474,aa:Gly,seq:ccc)
     gene            13545..14882
                     /locus_tag="PLK55_02790"
     CDS             13545..14882
                     /locus_tag="PLK55_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOZ35886.1"
                     /translation="MKIRYYEKIGLCAPRVLLPKDIDYKKWAVVACDQYTSEPEYWNS
                     VKEIVGSNPSTLNIIFPEIYLEDEDKSERIKSIVKNMKEYVSNKLFEDYNGFVYVERK
                     IKDKVRKGLVVALDLEKYDYRIGSTTLVRATEGTILDRLPPRIEIRKDAPLELPHIMV
                     LIDDSKGKVINIMSENKQDFEKLYSFDLMKNGGHISGYLISKKFEKKAARALEKLIVP
                     EKFKKRYKLEKAYSPLLYAVGDGNHSLATAKAVWEIIKKEAESKNEIMNHPARYALIE
                     LVNLHDESLEFEAIHRVLFNLKKDIIKEMKSHFEESFKFSKKSKEEVMEIIKTKKSKD
                     KHFIGLITDNPKKTGIIKIINPKSNIPVGTIQEFLDNFGRNGGFEKIDYVHGDDVLCT
                     LGQKPNNAGIYLPTIQKKQFFKSVIVDGALPRKTFSMGEAHEKRYYLEARKIR"
     gene            14949..15875
                     /locus_tag="PLK55_02795"
     CDS             14949..15875
                     /locus_tag="PLK55_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOZ35887.1"
                     /translation="MSFKEQLEKIKDNWFLLVLAIIIVMIMLFTMSGLGSISRGYSST
                     NYAMDVSSDSMSAYVGSPSYYGYNQPAGPSTFSPEQIYRQVIKNSNMGLEIKVGKFQE
                     TDAQLKSYISSKGAFILKENINTNTANNKYIYTGSYTIKVKVSDYDSVISQLKTLGKV
                     TSLNEGEDDVTGKYTNNKLELEVEKERLLRYKEMYAQATKMEDKLNLNDRIFNQERTI
                     AYLEDSLKSIEERVDYSTINLTIREKSDYIGIAFIKLATLVKAFVSSTNALLQFIAGI
                     LPWALVAGVVYFIVVAIKKRNKSKESLSQKKK"
     gene            15907..16362
                     /locus_tag="PLK55_02800"
     CDS             15907..16362
                     /locus_tag="PLK55_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35888.1"
                     /translation="MPPIKRPAGYMKYRQRRKQNIDRLRNIFKEHGANYKLFREKGFS
                     ASEVAGIEYNYLPKKEIIELFRNKNPKATAKQKESQLTYEQLEQIRRFRIPAKKLLEV
                     GYTPLELLAAKYSYADLRVAGVDVITLKKKVDAFKSQVKEISEIKKAKR"
     gene            16567..17751
                     /locus_tag="PLK55_02805"
     CDS             16567..17751
                     /locus_tag="PLK55_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ35889.1"
                     /translation="METLKRELLNHKLILSIEGPRRVGKSVLIKQLINYLIEKKVPST
                     NIFYFSFDKLDKKILDTLKDYEELSGISLREEKTYLFFDEIQKIKDWQSDIKIIYDNY
                     PNIKLVVSGSTLRVSKKESLAGRLVDFFVNPLSFEEYLIFSGKEKLLDSGMDYIFEKE
                     YNNYLFRQYPDIVLNKDIDSKQYLLLILQKVIFEDSEKYIKNVDKDILYKICMVILRD
                     PGEIINYSDLSKDLGVKRGEVSKYIDFLVNSSIIRKIYNFSNNSRKLEIKSKKFYPYC
                     TSLTRAITDNPDMSKIIETDVCFQLDAKYFLNNKGKEEIDFLLIDPITSKKIGVEVKY
                     RNLITNKDTNAFKSVIVKKLNLSKKIIIVKAESKLDFNTKEIIPIKYFSIFRHKSEFD
                     LK"
     gene            18006..>18192
                     /locus_tag="PLK55_02810"
     CDS             18006..>18192
                     /locus_tag="PLK55_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35890.1"
                     /translation="MTKKSNSALIIWAVVALIVGIFLGLLITNLTTTGDAKSATEVAP
                     RITLNHNANLILTQKMYN"
BASE COUNT         6829 a         2513 c         2940 g         5710 t
ORIGIN      
        1 cttcttgtct ataatatata ttagataaac tattgtttta tatatttaaa tatgatattt
       61 ataaaattct aaaaaggtga atataatttg ataccaaatt ctctaggcgc ggcagcagct
      121 ggctatgata gatcaacaac aatgtttagt ccagaaggac atctctacca agtagactat
      181 gcttcaaaaa ttatagaaaa aggaagcccc gcagtagcta tcatctataa acaaggaata
      241 ttaataattg cagacaaaaa aatacactca agtcttgtaa ttccagactc agtagaaaaa
      301 gtatttaaaa ttgataacca catctggaca acctgtgcag gcctaatagg cgatggcaga
      361 aggcttgttg attttgcaag acaaatgtct caagagaata ggacttatta tgaccaagca
      421 attgaggttg agactcttgt taaaaagata gcttcagtga tacaatatta tacacaatat
      481 ggtgggggaa gaccttttgg agtgtctctt attattgcag gttacgattc tttaggaaag
      541 cacatctttg aagcagagcc aagcggtgca ataaccgctt acaaggcttt agcaattggt
      601 cagaatagac aaaaattaat ggattactta gaaaaaagct ataaagaaaa tctaagcttt
      661 gaagatgcat tgacaatagg cataaaagca atatctttaa atctagaacc aaaacaaaag
      721 ctaaatgttc aaggcataga agtaattaac gtaacagaca atggtaacac tgatgccgtt
      781 gttccattag aaaaatattc aaaattatta taggtgataa attatggtaa agcttgacga
      841 cgctgttctt gcaaagtata gtagagatgg gcatcacttt gagattttag tggatccata
      901 tcttgcttgg gattttaagc acggaaaagc agatgttaat ttcgataaca tttttgcaat
      961 ggaagaagtt tatgcagatt cagcaaaagg gaaattagct tcagaagaaa ttgttaaaca
     1021 agtttttcaa actagcgatt ttctaacgat tgcaaaaaaa ataatacttg acggagatgt
     1081 tcaattaaca accgctcaaa gaaatgaaat gcttaaaaga agagaaaatg agattattga
     1141 ttttatagca aaaaatgctc atgacccaaa agagatgact ccaataccac cacaaagaat
     1201 aatcaatgct ctagaggcag gaaaatttaa atttaatctt tcaagaaaga aagaagacga
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggttcagtta tagttaatgt
     1381 tgaaaaacag tagagagagt gattttatga aaagaatagt aattcctgga gaactaatcg
     1441 cagaccagag aaagaaagtc ggcgagaatg ttttcattaa ggaaaataaa atatatagtt
     1501 cagtattggg attgttaaac gaaactgatg attttgtttc tattgtgcct ttaaacggcc
     1561 catacattcc acaagagaat gatcccgtag tttgtgttgt aagaaatgtt acagcaaacg
     1621 gttatatcat agatgtcaat tcccatagcg attgtttctt tccaaagtca atgataacta
     1681 gagaattaaa gattggacaa atgctttttg caagagttaa aggcgtggat ggtgttgagg
     1741 tttctgagtt agataatatt aatattcttc cagatggatt aatctattcg gtttcttcag
     1801 ttaagattcc aaggataata ggaaagaacg attcaatgtt aaatatttta aagaaatata
     1861 cttcttcaaa tatcttagtt ggaagaaacg gttggatatg gtatgtttca aaaaatggaa
     1921 atcttctaga gaaagcgctt tctttaattg taactaattc ccaaaaatca aatctaacaa
     1981 acactataga agaatactta aaaaataatc aataaaagag gagaataaaa tatgagtaat
     2041 gtaaaaagac cagatggaag gtcttatgat gaattgcgtc ccataaaagt tgaattaggg
     2101 acagcaaaga atgccgaggg ttcttgttat ttagaatggg gtaaaaatat agtgatagca
     2161 acagttaatg gcccaaagcc agttttacca aaacatttag caaatccaag taaagcaata
     2221 ataaactaca aatacagaat ggcaacattt tctgtatctg aaaggaaatc gccagttcca
     2281 ggaaaaagag acaaagagat ttctctagtt tctggtcatg ctctggaagc agcagtaatg
     2341 gttgatcaat tccctaacac catgattgat gttaatgttt tgattctttc agctgatgca
     2401 ggaacaagag ttgcagcttt aactgcagct agccttgccg cagcagatgc tggtctacca
     2461 atgagaggaa taataagtgc agttgcagct gggcgagcaa acaagcaaat tgttcttgat
     2521 cttgataaag aagatgaaga cacagaagat gcagtagata tggcttgtgc atttttaatg
     2581 ccacaaaaag aactagcgct tcttcagatg gacggttttg tagataagaa agactgttca
     2641 aagattttag aaactgcttt aaaaggtgca gagaaagtct atgagattca gaaaaaagca
     2701 cttctagaga aatatccagt ggaaggtgaa atacaatgag tgacattgat attttaaaaa
     2761 taataaaagc agacaaagca aagaaactat tgcaagaaga aaaaagattt gataatagag
     2821 gccttttaga ttctagaaat atagaaatta aaagaaatgt tatgaaatct gctgatggtt
     2881 cagcttgggt aaaactagga aacaccgaaa taattgcagg agtaaaattt gcagttggaa
     2941 cgccttatgt tgattcacca gaagaagggt ctttagtatt aaacctagag atgtctggaa
     3001 ttgcttcagc tgacatcgat acgggcccac caagcattga tgcaatggag tacggaagaa
     3061 tcgtagacag agtaataagg tcttcagaat gtgtagattt taaagaccta aacattgtta
     3121 caggcgagaa gtcttttaca gtctatgttg attgttatgc cttaaatgca gatggaaatc
     3181 tgattgatgc agcacaatat gcggcaatgg cagccttact tgattgtaag attccaaagc
     3241 ttgatgagaa taacaacatt gtcaataaag agtattctga caagaaacta aatattgaca
     3301 caaaaaagat agcagcatcc tttactttct ggaaaacaga aaacaagatt tttgcagatg
     3361 caacagaagt tgaagatctt gcaagcgaca caagatttac tcttggcgtt gtgggtagca
     3421 atgttgttgc ttatcataag gggggaggaa acggaaccct taagattgaa gacataaacg
     3481 taatgatgga tatagcttca aaaaaatatt tagaaataaa aaacaaaata tgtgaggaac
     3541 aatgagtaca aaaaaagttg gtaagactgg aaaatacgga gttagaggcg gagtaggtat
     3601 tagaaagaaa tatctacttg caatagacga taaaagcaaa aaagtttgtc caaactgcgg
     3661 aacaaaagac aagcttaaaa gagtagcaac cgggatctat caatgtcaaa aatgtaagac
     3721 aaaggtttct ggcggcgcat ataaatttaa aacaacaggg caataggtga aattatgtac
     3781 tattgtggta aatgtaatga agtttataac gagatgccag agagcaagaa atgtaaaagt
     3841 tgttctggta gaatatttta caagaaaaga gcaccaattg ttaagaaggt aaaagctgac
     3901 tgaaaagatg ttaatcacaa cttcaagaaa tgcagatatc tcccagaaaa gattttgtaa
     3961 atacttgtct gtgttttttc cagaagttaa gcacatacca agagggcaat ctcagctagg
     4021 aaagctcttt aataaggcaa actatcttgg cgatgatttt cttcttatag ttgggaaaaa
     4081 aaaagaaaat cttgagctan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     4201 cttggcgatg attttcttct tatagttggg aaaaaaaaag aaaatcttga gctaatggtt
     4261 tacaaaagaa agcagaagga gttttttcct gataagtctt ttcttttaac agatgtcttt
     4321 tacaagggcc caaaagacaa gatacaaagc acaaacgcca aaggcaagtt tttttacttt
     4381 ctagaaaaaa cagacaagga ctcagaaata aaggcaagcc agaaagaaag cgaggttgtc
     4441 tttaaaatca aaaaagaaat tttattttct tttaaaatcc taaaagagga aaaggcatga
     4501 ttgattacat tattaaagag gaattaataa tcaatacaag aaatccaaaa aagcttaaag
     4561 agcttttaga tattgagatg tctagaaaag actttaaaag atcagagtca aagattacaa
     4621 tagacaacaa aacaaaaact ctaaaaataa taattcttgc aaaagatata acagcctaca
     4681 aagcaacaat aaacaattac attaatctat tagaattgat taaaaaaact tatgaggtgg
     4741 atttatgagt gaaaatgtaa atatgtacag acagcaattg atgtttttga cacaacaaaa
     4801 gcagcaatta caattccaaa tgaatgtttt agaaaacaca ttaaaggaat tagaaaagac
     4861 aaaagagaag aaggtctata aaggcgtagg gaatgttttt atcttatctg ataaagaaga
     4921 tgttttaaaa gacacaaagg accttaaaga aacaatggac ttaagaataa aaaacattca
     4981 aaagcaagaa gacgtaattc taaaaaagat aaacgagaag accaaagcag aaagcaaaaa
     5041 caaatcagaa gataaagaac aaatgaacgg agtacagtaa gtacttcttt ttttctattt
     5101 ttatattaca atatataaat ttttcgtttt attgtatcta tattaaatta ataataggag
     5161 gaaattaaaa tggcaaaaaa agaaaacaaa tcactaatta tttgggcagt tatctgtcta
     5221 atagtaggtt tagtaatggg cctattttta acaacagtgg ttacaaccgg taaagcaatg
     5281 caggctttaa gtgaaaaatc aacagcaaat ttagaagaag caagaacaaa tttagtatta
     5341 aacttattaa aagtaaatac aattgaaaaa aatggcaatg gccaattaaa tattttaagt
     5401 caaggacaaa atggacaaat attaatggaa ggaaatacag gggtaactct tggaaccaat
     5461 ggtacatcat ttattatggg ggggactcct caagagaacg gaatttcgat ggcatctcag
     5521 aatgttaata ttaacacaaa tgcgtttaga gtttcagcac acggcgaaac agaaaatgcg
     5581 ttagctattt atcctttagg agattcagaa ggttccaaaa gaataactat tagtgtccca
     5641 ctaaaatata atgttgcaat ggatgtaaat tatggtgccg acaattatta cgcttgttta
     5701 acagacgaag gcaccctatt taaaagttat tcgccatgta actaaaacag aaagcaaaaa
     5761 aacaaaatca tttatttaag atagatgatt ttgtttccat cttttatttt tcttcttttt
     5821 gagtatttga ttaaatagtg tatcttgctc tttggaatgc ctagcttttt ttcaagatcg
     5881 cggtagctgt agtccttgta ttcgcgaagt attttttgca gtttttctac cttaatatca
     5941 ataggtctgc cttgttcttt tctcaagatt atagttatgt tctttttctt aataaattcc
     6001 tttaaagcct tactaattct ctcgtttgtt gagttagaaa tagtaatagt ctttagtctc
     6061 ttacattttg ctaatatctt agtaaggttc ttcttagaaa atgccctagt aacatgcaat
     6121 tcttcacaat tagaattaag ttgcgatatt tttaaaaaat ccgataacgt gtctttaatt
     6181 gtatacattt tttaacctct gtccaagatt taatataata tatatttata gaatagattt
     6241 ataaataaat ctttagtata atagggtttg tagaaaccct ctattactcc taatttttag
     6301 gtaacataaa aaatatatat gtctttcgac atatatattt tgagaattta tgttaaatat
     6361 attacaaccg ataagtttta ataccttttt caagcaagtt gaacgagttt ttggcgaagt
     6421 tagactgcct tttcattttt caaagtactc taacaagctt tacaataact ttgttcacgt
     6481 gtatttactg attttaaaag aaagacacag atgctcatat cgtcgtttaa cagaattgtt
     6541 aaacgacatt aatgttttac gaatgcttgg aatccagaaa ctaccgcact ttacaacttt
     6601 gcaaaaattt gtaagcagaa taagtaaaga cgttttccaa aaactagtaa tggcttgtca
     6661 caaacttatc aacagcaaag atctaattgt tgctgttgat tcaactggtt ttgtgaatgt
     6721 taatcccaca tattattacc taaacagatg tgggattaga ccaccttata aaaattttac
     6781 acaaacaagt attgttgtag atactaatac aaaacttgta cttaatatat gcacgaactc
     6841 gcatcgcaag aacgatacac aatcgtttat tcccctgctc gataaattaa agcacaacat
     6901 agttcgtgct ttagcagaca aaggatatga ttctagagag ttacgcgaat actgctggaa
     6961 taataatatt acagttcata ttccatttcg taagttgcct tgttatcaga caccgtctga
     7021 taaccgaata aaagcaatga agcgatttag aaccaaatcg tataataaaa gatcagttgt
     7081 agaatcagta atatctgcaa tcaagagaac aacaggtggt tttgtaaccg caacaaaaca
     7141 ggataaccag caaaagcagg ttatcctaaa agtattaaca tataatctag aaaaattaag
     7201 tagaaatcaa aaaattattt catttacaat tttgatctgc taaaaaggat ttctacagtg
     7261 ccagtataat aaaatataag tctaaaaaag gaaaaactca aatgatacaa tcttttctat
     7321 taagttttgg aataattatt cttttttcaa ctttaatgct ttatatcttt acaaaactca
     7381 agcagccacc tcttcttgca tttattctta caggtatctt aattggacca atggtcttag
     7441 gcctagttac caatacagaa gaaattgctt tactagttga aataggaatt gcatttttgc
     7501 tatttagtgt tggactaggt acagatttag atgaactaaa aaaaatcaat ctcggtttaa
     7561 tcattcttcc aattgcaaat ataataatta cattattagt ttttcttatt tttaaaaacc
     7621 ttttagcaat tgattttatt caagctttgt atttctcatt tattgtttct tttagttcta
     7681 caatgcttat agcaaagata ttattagata acttcaagat ggactctcta gatggtagac
     7741 taacaatagg ggtattatta gtcgaagact taatcgcaat tctagcaata cctttattaa
     7801 aaaatataag caacctaaca gttggtttag tcggcattat ttttgttaaa gcagctttac
     7861 tagtagtaat agcaataatc ttaaacaaaa caatataccc ttatattata aaaaacgcgt
     7921 ataaatcaga tcaaagtttt ttcatgctat caatagccag ttgttttcta tttattttaa
     7981 tttctgtaat tctggatttt ccaatggcaa tcggcgcatt ctttggaggt cttgcaattt
     8041 ctatataccc ttataattat gagatttcca gcaaaatttc cggcattaga aacctattaa
     8101 caacaattgt ttttgtgtct cttggcatga aattatcttt taatttctct gataatcttt
     8161 tgttattaat aatattattg attttagttt tggtaattaa accaatcatc cattttttat
     8221 ttgttttatt ttcggggtat ggttcaaaga tagctcttgg agtttctact tataaggcgc
     8281 agatttcgga attttcttta attcttgcaa ttcaggggtt ttcgttatta caacttacaa
     8341 aagggcaata cagtgcagta attattgcaa catctttgtc tatgctttta acgccataca
     8401 ttatcaattc tcaacaaaag ttatatttaa aatttataaa gtccttttct aatctagaca
     8461 aaaacaaaaa attcactaaa gagattgatt ctttaaatag cgtttctaaa gatatagaaa
     8521 atcatttagt aattgttggt gcagacgtta caagcgacgc cataataaaa ttattaggta
     8581 aagaaacaaa caacccaata gttttagttg atcttaatcc tgacaaaatt tctggtatca
     8641 accacaatag aattagtggc atttgtggag atattaatta tcctgatgta tttaatgcag
     8701 caaacataaa aagcgcaaag cttgcaataa ttacagtttc tgagtttgat acaacagcaa
     8761 gatttataaa aaatgtaaag aaagttaatg cggacttacc aatctttgca agagcacaca
     8821 cccgtaaaga ggcctataag ctctatgagc ttggtgcaga gttagtgatt atcccaaaag
     8881 tattagagtc taactatctt ctagaaaaga cctatgattt tatgttaaat caaaatacca
     8941 aaagcacagc acttagatca aaatatctag aatatctaaa aaaagaagct gcaaaaaaca
     9001 aagaaacaac cagaaagaaa gaatcaaagt ttggtttttg attctgtaat taactcattt
     9061 attttttcat caagtctttt taaaaaatat tttttgtctt gatatttgtg tttttctaat
     9121 tttgttttat agtaaggaat gttttttata aatctcctta ggtttattgc gtctattttc
     9181 tttgtagaaa gaccatattc tagtttttct aaataatatg catttgttaa ctgctcaaat
     9241 tgatgtttta ttggtatgga gtatattggt tttttgaaat agatcgcttc agtgattaaa
     9301 gtgtgcccac cattagttat aattgcctta ctttctgcaa tttctttaaa taaatttggt
     9361 tttgcttttc taaattctaa attttcatca atcttattta cattataccc aaatataaca
     9421 aatttttgat ttttaatttt ttttaaagtt ggaaccatct ttgctttgtc cataaatcta
     9481 ttatatacaa gtatatagtc tccgtctttt gctttgtatt tctttatatc gtcaagaaat
     9541 attggatcaa atacttctgt gttcttttta gttatttgtg ttctataaaa gcacaatcca
     9601 aaataaaaat ttgcaaaagg ataaacaaac ttagaaacgc tttttgcctg taagaaatcg
     9661 gccgcatctt tctgtttgaa ttcaacgtct cctctaaaga tagttgcgtg attatcaatt
     9721 tcaatggttg gtagttctag aaacttaccc ccccacaaag aatacctctc cagatcagaa
     9781 ataacaatat ctggttttat ttttttaata agtagcgtgg ctttctttag attattaaaa
     9841 ttgtgtttat taaagcattt aaaaaaactt ttaaatgcct tgacactatc gtttgtgtat
     9901 tcgaaatata cttgtggaac aatgtgtatc tttaactgtt tatatttgtg ttgtaaaaaa
     9961 tctgcaactt ttttgtctgt gataatttct atattgtgtt ttttgtgtaa taaatgatct
    10021 ataataggga tacttctagt aacgtgtcca aagccgtctg catttattgc atatacaatc
    10081 ttataacttt tagtcatatt aatattaaga aataaagcag tttttaaatc tttttaaata
    10141 tctttttttt atgatagtat taatttgtgg tttttcggct tcaggtaaaa gttatcttgt
    10201 taattctgtg gctaaaaaac ttaaatataa gcacatacac acctccgata tccttaatca
    10261 gttcgctagc ggtattccag aaaataaaat acaaattgaa aacacaaaaa tgaataacgg
    10321 ctggtatgag ttttccggtc ttgataaaaa aagagataag agtaagtttt tagataaaaa
    10381 gcttgataag tttttaataa aactagttaa taccaaagat aatttagtac ttgactcttg
    10441 gaccttacca tatctggtca agaaaaatcc aaagataatt aagatttggc ttgatgcaac
    10501 agaaatagaa agagcaaaga ggctttcttt aagagacaaa atttctttta aagaagcaca
    10561 cacagtacta aaagaaaaag atagtttctc aaaaacacat tttcaaaaac tctatggttt
    10621 tactttagga aaagacaaga aagtttttga tttagttatt aatacaaata agctatcaat
    10681 taagcaagta gaagaaaaaa caatgtcttt tcttaaaaag aaaataaaaa ttaaataatt
    10741 ctttttctaa gtttttctat aacagaataa ttaacagtat attctcctaa tttattttca
    10801 gggaaatgac ttagagaatg gaaatcagac ccgccagttt ctataagccc ctttgccttt
    10861 acaatttcct ttatttttgt aattaatttt tcatcttctt ttggatgcgg tcctgttgtt
    10921 tcaattccag caagcccttg ttctttaagt ttagataaat atgcttctat atcatttttt
    10981 gtggcccctg gcaaccacca aggatgtggc aataccgcaa caccattagc gttaataatc
    11041 tcagaaatta ctttttttgt agagtatctt tttggtttat agtcaatttt gattttttta
    11101 attattatcg agaatgcatc ttgaagtgtt gggacatatc ctttcttttt aagttctaga
    11161 gccagatgcg ttgcttgtaa cagcttgttt ggcccaacaa attgcatcat atcatttaaa
    11221 tctatactat atccgttgtc ttttaaccaa tagaattttt gcatagttac atcgtttata
    11281 gatttggcat tttcttccat aatcttaagc aatggttcat aagtagtatc aaagaaatat
    11341 cccaaaattt ctgtgctctc acaattaatc tcaactcccg ttattagctc aatattttct
    11401 ctttctgcaa ttcttcgcgc aattggtaaa gcagctatag aattgtggtc tgtaattgct
    11461 aaagctttta atttcttaga aattgctttt tcaatcaaat actcaggaga atgaaaacca
    11521 tctgaaaagt aggtgtgagt gtgtaaatca catttcatat ttagtttttc gcctctactg
    11581 catagtcttt gttgcaagct taatgtcttc gccggttatt gtttttcttt tggcgtgttt
    11641 tgcaagttca attgcctgct tagagatgtc cattgcaacc tcagaaagta ttttactaag
    11701 ttcttctgcc gcatcttctg aaactctctc tgctcctgcc tttcttataa gcctatcaat
    11761 tgctgccact ggtaaatctg tcataaaata acccccatat ttaatttaaa tgaaatttaa
    11821 cgctttttat aataaaatga tacttcaaaa gctttttaaa caaaacccct aaaatatata
    11881 taagggggca atttatgaca ttgatggatt ctattctttt gcagattaat caaaagaaga
    11941 caagttttaa tgagctactt acacgaatca tacctaatta ttcaagtaca gaatctgcta
    12001 gagccgctct ttcaagagtt ataaaaaatc tagtatcttt tggttatatt caaaagactg
    12061 aagatttttt tgaaataaca gaaaaaggca aaaaaatagt agagtcaaag cttcagaaca
    12121 aaatgttaaa taatattaat agcattaacg atcttttaca gaaatctcaa cctgcaaagt
    12181 ctttacaata tcttgatgac attgttagaa atcttcagat atttctagaa agatcaaaag
    12241 aagatacaac tcttcttaag attggtaaga ccagtgcaac attttatatc tctgatctag
    12301 aggatattaa aaaaaatgta gaaacaagac taattaattt tgaacagtct ttggataggc
    12361 agattatggc tcttaaggat cttaattttg aggatcagtt tattattaat cttgatgaaa
    12421 atgccttttc tttgattaag gaactatcag aaaacaataa tatttcagaa ctagtaattg
    12481 attgtgataa aacagacatt aactctggaa tgctttttga aacccattct gaatttacaa
    12541 aaaaaccaga taatgttttc gtgcttaagc tttcagatat tgctattttg aaaaaactac
    12601 ttctctcaag attagatatc attatgcaga ccagatttaa agtatatatt aaagaaatag
    12661 taatttatct taaaataggc aaactttact ttactggccc atataatcag atattagcaa
    12721 ttaaagaaaa ctatcaaaag aaaacaaaaa gtcaggagaa aaaataaatg aaaattgaat
    12781 atataggtca ttcttgtttt gttataagtt atcaaaatca caatattttg attgatccct
    12841 ttatttctaa aaacccaatg cttaaagcaa atcttgaaaa tttcactcct gatttaattc
    12901 ttgtaagtca cgatcatttc gatcatgttg gggatacaga aagtattgca aaagcgaata
    12961 acagtacagt tgtctgtgaa aacgctcttg gcgaaaaact aaaagaaaaa gggttatggg
    13021 ttctgtcagg aacaattgga gataccataa actacaaagg tatagaagta acttttgtta
    13081 aagcgattca tcgttcagat aatggaatag ccactgggtt aattataaaa acagaagagc
    13141 acacaatata ttttgcagga gatacagatt attttataga aataaaagaa attgcagaaa
    13201 attttaagcc agatattgca attcttccaa taggcggagt ttatacaatt gatccaaaag
    13261 gcgcaattga gtttttaaaa gagctaaaac caaagtttgt aattccaatg cattataaga
    13321 cctttatgaa tctttatggt acgccagaac aattacaaga actaatagaa aaagaaaatc
    13381 tagatataaa acaaataaca ttagattttg gtcaatcaat agaaatttaa aaaagctctg
    13441 caactatagt ctagtggtag gatgcaagct tcccaagctt gcggctcggg ttcgaatccc
    13501 ggtagttgca tatgtttaat tctgaaaaaa aaaggagata taaaatgaaa ataagatatt
    13561 atgaaaaaat cgggctttgt gctccaagag tgttattgcc aaaagatatt gattataaaa
    13621 aatgggctgt tgttgcttgc gatcaataca cctcagaacc agaatattgg aattctgtta
    13681 aagagattgt aggaagtaat ccttcaacat taaatataat ttttccagag atttatctag
    13741 aagatgaaga caaatccgaa agaataaaat ctattgtaaa gaatatgaaa gaatatgttt
    13801 ctaataaatt atttgaagac tataatggtt ttgtttatgt tgaacgaaaa ataaaagaca
    13861 aagtgcgaaa aggattggtc gttgctttag atctagaaaa atacgattat cgcataggtt
    13921 caacaacact tgtcagagca acagaaggca caattctaga taggcttcca ccaagaatag
    13981 agattagaaa agatgcgccg ttagagttgc cacacatcat ggtattaatt gatgattcga
    14041 aaggcaaagt aataaatatt atgtcagaaa acaaacagga ctttgagaaa ctttattctt
    14101 ttgatcttat gaaaaatggg gggcatattt ctggatattt gatttctaaa aaattcgaga
    14161 aaaaagcagc aagggcttta gagaaattaa tagttcctga aaagtttaaa aaaagatata
    14221 agctagagaa agcttattct cctcttcttt acgcagtagg cgatggaaac cattctcttg
    14281 caacagctaa agcagtctgg gagattatta aaaaagaagc ggaaagtaaa aacgagatta
    14341 tgaatcatcc tgcaagatac gctttaatag agctagttaa tcttcatgac gagtctttag
    14401 agtttgaagc aattcatagg gttttgttta atcttaaaaa agatataatt aaagagatga
    14461 aaagccattt tgaagagtct tttaaatttt ctaaaaaatc aaaagaagaa gtaatggaaa
    14521 ttattaaaac caagaaaagc aaagacaaac attttattgg tttaattact gataacccta
    14581 agaaaacagg cataataaaa ataataaatc ctaaatctaa tatccccgta ggaacaatac
    14641 aggaattcct ggacaacttt ggaagaaacg gcggctttga gaaaatagat tatgtacacg
    14701 gtgacgatgt cctttgtacg cttggccaaa aaccaaacaa tgcaggcatt tatctaccaa
    14761 ccattcaaaa gaagcaattc ttcaaaagcg ttattgttga cggagccctt ccccgtaaaa
    14821 ccttttctat gggagaagca cacgaaaaac gatattatct agaggcgcga aaaattaggt
    14881 aatctcctaa gtattacacc cagtatttaa ataatatggt ttaagaaata ttatttggag
    14941 gaaaaaaaat gagttttaaa gaacaattgg aaaaaataaa agataattgg tttttgctgg
    15001 ttttagcaat aataattgta atgattatgt tatttacaat gtcaggctta ggatcaatct
    15061 ctagaggcta ttcttctaca aattatgcta tggatgtttc ttcagacagt atgtctgctt
    15121 atgttggatc tccatcatat tatggttata atcagcccgc ggggccatct acattttcac
    15181 cagaacagat atacagacag gttataaaaa actcaaacat gggtttagaa attaaagttg
    15241 gtaagtttca agagactgat gcacaattaa aatcctatat ttcaagtaaa ggtgccttta
    15301 ttcttaaaga gaacataaac acaaatactg caaacaataa atacatatat actggaagct
    15361 acacaataaa agtaaaagta agcgattatg attcagttat ttctcaactt aaaacattag
    15421 ggaaagtaac aagtttaaat gaaggagaag acgacgttac tggtaaatac acaaataata
    15481 aattagagtt agaagtagaa aaagaaagac ttctaagata taaagaaatg tatgcccaag
    15541 ccacaaagat ggaggacaaa ttaaatctaa acgatagaat ttttaatcaa gaaagaacaa
    15601 ttgcatatct agaggattct ttaaaaagta tagaggaaag agttgactat tcaacaatta
    15661 atttaactat aagagaaaaa tcagactaca taggaattgc ctttataaaa ttggcaacat
    15721 tagttaaagc ctttgtgagc agtacaaatg cgttattaca atttattgca ggaatacttc
    15781 cttgggcgct tgttgcaggc gttgtttatt ttattgtagt agcaataaaa aaacgaaaca
    15841 aatcaaaaga gtctttaagc caaaagaaga aataattttt cttcttttct tttatatttg
    15901 agtattatgc cgccaataaa aagaccagca gggtatatga aatatcgtca aagaagaaaa
    15961 caaaatatcg ataggcttag gaatattttt aaagaacatg gcgcaaacta caagttgttt
    16021 agagaaaaag gctttagtgc ttctgaagtt gcaggaatag aatacaatta tttgccaaaa
    16081 aaagaaataa ttgagctatt cagaaacaaa aatccaaaag ccacagcaaa gcaaaaagaa
    16141 tcgcaattaa cttatgagca actagagcag attagaagat ttagaattcc tgcaaaaaaa
    16201 cttcttgagg taggctatac gcctttagag ctactagccg ctaaatattc ttatgcagat
    16261 ttaagggttg ctggcgttga cgttattact ctaaaaaaga aagtagatgc ctttaaatcc
    16321 caagtaaaag aaatctcaga aataaagaaa gcaaaaagat aatttgctat ttttacaatt
    16381 tgcacttcat ttttaacaaa aagtcagact ttttgcttaa attttgatgc aaaaagcaca
    16441 attttaacga tttctttaaa aacatttctg gcaataacat atttgataaa tatgtttgaa
    16501 atatacaaaa ttcaaaatca acattgggaa aataagttat atgattttcc aattaagaga
    16561 agaattttgg aaactttaaa aagagaatta ttgaatcata aattaattct ttcaatagaa
    16621 ggcccaagaa gggttggcaa atcagtttta ataaagcaat taataaatta tcttatagag
    16681 aaaaaagtgc cttcaacaaa catattttat ttttcttttg ataaacttga taaaaagata
    16741 ttagatacat taaaggatta tgaagaatta tccggtatat cattaagaga agaaaaaacc
    16801 tatttatttt ttgatgaaat tcaaaaaatt aaagattggc aatctgatat taagataatt
    16861 tatgataatt accctaatat aaagttagta gtaagtgggt ctacattaag agttagtaaa
    16921 aaagaaagtc ttgcagggcg attggtggat ttttttgtta atcctttatc ttttgaagaa
    16981 tatcttatat tttcgggcaa agaaaaacta ttagattcag gaatggatta tattttcgaa
    17041 aaagaatata ataattattt atttagacaa tatccagata ttgttttaaa taaagatatt
    17101 gattctaagc aatacctttt attaatttta caaaaagtaa tttttgaaga ttctgaaaag
    17161 tatattaaaa atgtagacaa agatattttg tataaaattt gtatggttat attaagagat
    17221 ccaggagaaa taattaatta ttctgattta tcaaaagatc tgggcgtgaa aagaggcgaa
    17281 gtttcaaaat atattgattt tttagttaat tctagcatta taagaaaaat ttataatttt
    17341 tcaaataact ctcgaaagct agaaattaaa tctaaaaagt tctatccata ttgtacatca
    17401 ctaacaagag caataaccga taatccagat atgagtaaga ttatagaaac ggatgtttgt
    17461 tttcaattag atgcaaaata ttttttaaat aacaaaggta aagaagaaat tgatttttta
    17521 ttaatagatc caatcacttc taaaaaaata ggcgtagaag taaaatatag aaatttgatt
    17581 acaaacaaag acacaaatgc atttaagtct gtaattgtaa aaaaactaaa tttaagcaaa
    17641 aaaataataa ttgtaaaagc agaatcaaaa ttagacttta atacaaaaga aataatccct
    17701 ataaagtatt tttcaatctt taggcacaaa tcagaatttg atttgaaata attaattttg
    17761 ctgatgaata tgtctaaaca agaaaaaaat aaaaaagtta aaatttttta gaaaaactaa
    17821 acaaaagaag tacagacgca aagaataaat caaatgttct atcatttaag agtatataat
    17881 ttctattaag cagttctaat tatatccaaa tattaaaaaa tcgatatatt tataaaatcg
    17941 cccccccccc catagatata ataacattaa ataattaaaa acaaaaatta atggaggaaa
    18001 ttaaaatgac aaagaaaagc aatagtgcac taattatttg ggcagtagta gctctaatag
    18061 ttggtatatt tttaggatta ttaataacaa atctaacaac aacaggggat gcaaaatctg
    18121 ctacagaagt ggctcccaga attacattaa atcataatgc aaatttaata ttaactcaaa
    18181 agatgtacaa tt
//
LOCUS       DAOOON010000015        17833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_3847_length_17833_cov_19.021037, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000015 DAOOON010000000
VERSION     DAOOON010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 17833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17833
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3847_length_17833_cov_19.021037"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1515
                     /gene="gatD"
                     /locus_tag="PLK55_02815"
     CDS             178..1515
                     /gene="gatD"
                     /locus_tag="PLK55_02815"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOZ35891.1"
                     /translation="MNISNNDRGKLIKITKKNITHLGHIVNIANNELTIKLKENGYNI
                     SIILDKDTKLEILEEKKEIGKTHKLDIKQNSSLPSICLVSTGGTIGTHVDYKTGGVNM
                     SRTPEEILSTVPELLEIVNIKKIVPVIQKASEDICYKDWQEIAKAVFEQLNDKEIDGI
                     IISHGTDTLSWTGTALSFMLSNINKPVLMVGAQRSPDRASFDGAMNLVCAAHFIKEKI
                     PGVYTSMHGSMSDDFCNIINTTKCRKMHSSRRDAFKPINDTPLARVYFSGKIEFLKDR
                     KKYELLPKEKPKLDISFSDSVAILKTYPNSNPEVIDWYISKGYKGLIIEGTGLGHVPT
                     NDETNKLADKTKDWLPYLEKATKKGIIVIMTTQCLFGRVNGNVYSNLRNITKSGVEYL
                     EQHDMLPEVVCIKLGIALARFKTKEDILKYMSKNISGEITKKEVPFDFDALDT"
     gene            1532..2872
                     /locus_tag="PLK55_02820"
     CDS             1532..2872
                     /locus_tag="PLK55_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ35892.1"
                     /translation="MVLKESEKVELKKSTSELKDAIISICAILNKNQSGKLYFGIKDN
                     GEIIGQNIGKDTLRDIASEISRAIEPRIHPDIKKIILNKKECVVVEFTGSSNLYFAYG
                     RAYIRVSDQNQQLDIENLKNRIISQSDIIWDKQPSRNNVKEINTTQLKTFIKKANQNG
                     KINFKFTNTKDTLIKLNLLNKQKLSNAGNVLFSKTANIELQYAVFKTKEKSDFIDIGL
                     KKGNIFELIDFSKKYLFEHLNWSADLTKTNRIEKLEIPERALFEAIINSFCHKDYTII
                     KSNEIAIYPDRVEIYNPGKFPKEYTIKDYLSGRGKSILRNPSIAQILYYFDYIENWGS
                     GLRRINEYCKGQKIKVEYRLEPNGLSVIFYRPKEGVNVPVNVPVNVPVQENNRANLIL
                     NDIFKTPKIRIKELSIKFNVAEKTIKRAIQKLKKEKKIKRIGSDKNGYWLVLKK"
     gene            2883..4814
                     /gene="gatE"
                     /locus_tag="PLK55_02825"
     CDS             2883..4814
                     /gene="gatE"
                     /locus_tag="PLK55_02825"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOZ35893.1"
                     /translation="MDLEDIKKIDYNKIGLACGLELHQQLDTGKLFCKCKSKLIKDNT
                     KPDRIIKRRLRAVYSEAGEFDKTALNEQKKAKTYVYYFYNSCNCLVEADCQPPIRTNP
                     DALKIAVIISKLTNSQLVDKSIIMRKQVIDGSNVSGFQRTAMISTDGHLDFDFGKIRI
                     DKILLEEDAARAIERKDTEVIYSLDRLGIPLIEMVVWHDMHTPKDTQKVALFLGQLFR
                     TTGFVKRGLGSIRQDINVSIADGARIEIKGCQDLQMIPEIIDREILRQINLLELKEEL
                     KKRKIKEIRLSEINLTNIFKNTSSKAVKASLENKKEMYGFLISGFSGLLGKVVQPNRR
                     VGTELSSILKAKTTLKGLFHADELPNYGITEAEINKVKAELKVTDKDSFILVMCDKKE
                     ISRVKEILEERLNQFIEGVPKETRIVNIEGNTEYQRPLSVGGARMYPETDLLPIVFTE
                     ELLTEAKEDMPLSVEERKKLYLEKFKISNQLAEKMVLDNYAVTFEKIIKANPELNPTQ
                     VAVFLLEDLTNLSRDGKIDLENVTNEGLLEFFSYKNFNKIPKSKLVEIYLKFNNSDYS
                     LEDIIENLGVLKELDNIDVDKILSEIIEENKEKIIEQKERAKGLLMGRLMSKTRGLLN
                     GKEASELIDKKLAAFLKKH"
     gene            4863..5546
                     /locus_tag="PLK55_02830"
     CDS             4863..5546
                     /locus_tag="PLK55_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35894.1"
                     /translation="MDNRRRNKYPNNRYTKTNVQRSKKITPHQRKEVTEKANRNGFVL
                     ETIPANNYQDNSTYQERKESRNFFKDMYDKNGYYSYLWTPFIVLIVTIFFDFILQAIA
                     FSLDSTMMLIIKESLFFLVFSVLVFCINVASIIFMGLSTSKHNVPFNVAWYRIFRIIA
                     IIFLLESALTIIAFFVFPSQYIQITLFTQSMKLTYLIYLIAWNMIKAVVYMIMMSLAY
                     LLFYKLKFV"
     gene            5603..7198
                     /gene="metG"
                     /locus_tag="PLK55_02835"
     CDS             5603..7198
                     /gene="metG"
                     /locus_tag="PLK55_02835"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOZ35895.1"
                     /translation="MKTRIVTAALPFVNNVPHLGHLAGSHLPADIFSRYSRLKGHNVL
                     FIGGTDENGTATEFAAQQKGITPKELCDHFYEVHKKLYSDFLISYDNFSRTSREIHHK
                     IVQEFFLDLYNNKCITEKIIKIPYCNSCKKGLADRYITGTCPKCGYDKANGDQCESCG
                     SVLDPIELVTPFCSVCKTKDIVFKDTKHLFLDLRNLTKDIEYWIDNNPHLRPQVKALA
                     KGWITQGIKERCITRDLTWGIKIPISGYEDKVFYVWFDNVIGYVSGTAELIGEDNAKK
                     LWQNKETETYYFVGKDNIPFHTIFWPGQIIGQKKLVLPQNVVGLQYLNFEKQKFSKSK
                     GVGIFLDKAINSGIPIEYWRFYLTYILPETKDSEFMIDEFFDRINKDLIGNYGNFVNR
                     VLNFIYTKRQGKVPEIKEKDANFEKQILESIDKIEKHYDACDFRYALYELLKLSDLGN
                     KYLNEKEPWKTEDTNSLAYGFEIIRLLSIYFSPILPESSKKISSFLNTNNQNIEIDTK
                     EKKINKPEILFRKLEDTEKEKLM"
     gene            7212..7742
                     /locus_tag="PLK55_02840"
     CDS             7212..7742
                     /locus_tag="PLK55_02840"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOZ35896.1"
                     /translation="MIVKEKCKSLYAPWRQEYVSKVTDEKESTKDKEVCPFCSKNLDK
                     KYLVLETKKSFIQTNKYPYAAGHLLVVPKRHINQVIDLISIERKELFNLIDLSVFMLD
                     KYMRPEGYNVGCSIGRIAGESIQHLHFHILPRYKGDVGWNRLCDFNVISISPIDLAVD
                     LRKIIQKEKLAKKFGL"
     gene            7893..8393
                     /locus_tag="PLK55_02845"
     CDS             7893..8393
                     /locus_tag="PLK55_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35897.1"
                     /translation="MMTVLKRLFRKKTSAGEVEIDETIVKETNQLSSTAVIKRCDFRS
                     KDINWLEGHIQAVDDVVNKNSLGAKTKNHFEIDGRRYFKDDTTRVLFNRLYENYTAYY
                     YTLTEYLSLRKELLKYNGLGSESFFVAGQIKDTEEQMVAIEKKLNIYLDNITKIKQRI
                     RQYVGL"
     gene            8478..9479
                     /locus_tag="PLK55_02850"
     CDS             8478..9479
                     /locus_tag="PLK55_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOZ35898.1"
                     /translation="METFFQVVILFILVVLSAFFSAAETALMSIHFVRVKSLFKKGKA
                     GSKTLYNLKKDPHKLIVTILIGNNIVNISASAIATSLFLSLFGSEGVAIAAGVMTLII
                     LTFGEILPKTFAIQNAEKISLLVAVPIHYLSILFYPLIIFYSSITKLVSRWVRPKVEK
                     KISEEELKIILTLSREEGILSRGAAKIMHKVLDFEDTPVIKVMTPKEQMKTIDGDLKI
                     DEAIDVISRYGHSRYPVYFEDEDNIIGIVDVDDVLRAIKEKKSKELIKDIIRPIEFVS
                     PEKEIGDIFYDFEDKSVLMAIVVDKSGEVIGLLTIEDILEEIVGNIFDKSNRKKRRN"
     gene            complement(9476..9946)
                     /locus_tag="PLK55_02855"
     CDS             complement(9476..9946)
                     /locus_tag="PLK55_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOZ35899.1"
                     /translation="MQIFVIDKNPNKAARMLCDKHVVKMIVESAQILSTVHHLYDSPH
                     KHLVYKKTHEKHPCVIWAGKSKENYLWLLEHLSELIKEYEYRYCKHHKTTEIKKILET
                     PPKSLLSIGLTEFAQAIPEEYKQKDAVKAYREFYKNEKAHFAKWTKRKKPAWFN"
     gene            complement(9950..10189)
                     /locus_tag="PLK55_02860"
     CDS             complement(9950..10189)
                     /locus_tag="PLK55_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35900.1"
                     /translation="MGIFDAFKKKEKTEEEKAKEAELQKLKAKPAAQIDIANSKYPDQ
                     TCAVCSQKGCDKKWGGQYWHKKCLRSVKGMARDMV"
     gene            complement(10215..10287)
                     /locus_tag="PLK55_02865"
     tRNA            complement(10215..10287)
                     /locus_tag="PLK55_02865"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10251..10253),aa:Gln,seq:ctg)
     gene            complement(10317..10913)
                     /locus_tag="PLK55_02870"
     CDS             complement(10317..10913)
                     /locus_tag="PLK55_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HOZ35901.1"
                     /translation="MKKIKIAIDLDDTLVPTVDLLIKEMLKEKSFPAFRGYHSIKDFS
                     FEDEMALYRYIRKTINKQDISEFKPMPGSVLAIKKLAKEFELYLVTARTKNVKKHTRD
                     WISHHFPKMFKKIIFGQYRKEGDFKKTKGEICKELGISLIVDDRIDYALDCYNHGVKA
                     IILDYKDNYAWSKGKFPKSIKKARDWKEVYEIIKKTKF"
     gene            complement(10923..12059)
                     /locus_tag="PLK55_02875"
     CDS             complement(10923..12059)
                     /locus_tag="PLK55_02875"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOZ35902.1"
                     /translation="MSEKEFKVTPWEIEGAVDYQKLIKQFGTNELNKQMLEKLRLKTK
                     SKKLHPFFRRGFIYSHRDFDKILEDIDKQNFFIYTGRGPSGKMHIGHLIPFIISKWFQ
                     DEFDCNVYIAMADDEKFVFKDKLTIEEMEKNKKDNFLDISAVGFNTDKTFFFSDMEYI
                     QKLYPVALQFAKRTTFSTAKAVFGFNNDNNLGQFFYPAIQVVPTTFEKKHCLIPAGID
                     QDPYWRIQRDNAEKLGYKKVVAVHNKLLPPLQGIDGKMSSSVEETAIFLNDDLKTIEK
                     KINKYAFSGGQPTLEEQRKKGGNTDVCVVYSWLEILFEDCDEKLEQRKKDCKSGKILC
                     GECKKYLAEKISSTLQKHQDAKIKNSPLLDKYKYSGKLAKIMWQ"
     gene            complement(12131..>12724)
                     /locus_tag="PLK55_02880"
     CDS             complement(12131..>12724)
                     /locus_tag="PLK55_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35903.1"
                     /translation="SLLCALEKKEELKERLKETPKIKDKTTFEQIISFLFKTEKKYLE
                     FYIDIVHFLDGFFTKEEIIYYLNRLHPETKKDNIEKIFYNFISLLEKEMLIDYIDSKK
                     NHNSHYISALPKINNLFLMYSGSQKGYILGKNNKLEEVPKDVFFFFVFSKGIFMLREI
                     ANKLHLLFKDTKEFSKENYLKSTNKIYNTLKHYGLCK"
     assembly_gap    12725..12824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12825..13290)
                     /locus_tag="PLK55_02885"
     CDS             complement(<12825..13290)
                     /locus_tag="PLK55_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35904.1"
                     /translation="MKQKPQKIIFDYLEGKYFENYNQNFQNIIEKIKTVYPNIKIEAL
                     KKKTCRDEKFKEKNLQKITDIQKYTQRKEQKFIVVQDLMDLPEIKNILTQEIYDFWFY
                     NYRSSALKEILKLKKDYFDLNYYLTCDFEALSFEDAKLLAENGLEKLYLQLKI"
     gene            13621..14628
                     /locus_tag="PLK55_02890"
     CDS             13621..14628
                     /locus_tag="PLK55_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ35905.1"
                     /translation="MQEIDKFDMRFLVELEKDSRQPISNIAKLLKTSQQVLSYRLINL
                     EKRHIILGFYTVINFVKLGYTSYRTMIRLTNISPEIQARLISFIKEDNNVSYLAECTG
                     HWDLIVDYLAKNIVQYYSFLKDIREKFPENIGSIDVLTTLENIYFGRDYFAKKYREIN
                     KKSFIDTDYLTTEKLDNLDLKVLYEISKNARVGAMELANKVKTSPNTIIYRIKSMQDR
                     NIIEGFKPYLNIEKTGRFAYKSLIKFNNINEQAEELLITFLQNNLCVFDITKVIGYWD
                     FEIDFEVKNSDEMIDFTRTVRENFKDLIKEFETTPIYKEHIFNYFPGDLLSPEKKSKK
                     K"
     gene            complement(14629..15156)
                     /locus_tag="PLK55_02895"
     CDS             complement(14629..15156)
                     /locus_tag="PLK55_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35906.1"
                     /translation="MVIYSIFIFKFYRFIAKRDIVSISLEKYKDAKNSFFKKLFYNIL
                     YVIKYLFFTPIIIFFSFVVLTIILSFLAEGLTIETILLTAIALIGAIRITAYHDEDLS
                     RDLAKMLPFTLLGIFLVNVSYFSMPTVIELLKDIPSKLSLLVYYFVFIVIIEFVARMI
                     FSITIKIFPKKEKTE"
     gene            complement(15246..17156)
                     /locus_tag="PLK55_02900"
     CDS             complement(15246..17156)
                     /locus_tag="PLK55_02900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOZ35907.1"
                     /translation="MKAQLYFRHDSFRKGQKDIIRDIYESISLGKNILVHAPTGTGKT
                     DSALSAAITLAIEKDLKVLFLTPKISQHKIALEVIDDLNDKYDLKIKAIDFVGKKNMC
                     IEPVISRTDSNFYEICLMACKKKQCPFFENIRPSNKGQRDLLAYLLEKKLSEKEVLTH
                     LEIKKIAEELKSATGERQPVCAYELAKLFAKSCRVIIADYYHVFSSKVMESTLSEIGV
                     NLEECIIVIDEAHNLEDRLLKLISKSLNTNTLHRAIKEAKEIKNNKLKNLIEKQLDHI
                     ERIAEKKLEKIKEDRIEKEEILIKEIREDVISVIEELDESGLKYIEDLKESSSALISV
                     GLFLEEWIKEKDAHIRFIRKEKNGISVKYNALDVSPLTKKIFENCYSSILMSATLTPL
                     SMYTDILGLKKEETTTKEYSSPFEKEKRLNLLITDVSTKYTQRNNLEYKKIASHVEKC
                     VNAIPGNCVVFFPSFEVLGDIIPLINCNKPTIVQEERMDSKDFEEMILGFKKHSKKFG
                     SVLMAVMGGKASEGIDLPGNLLIGAVIVGIPLAKMEIETKAKIDYYEIKFRNGWNYAY
                     IHPAIQKVIQSAGRVIRTDTDKGVIVFIDARFHWENYRKCFPKDMQFVISKDLKIEIE
                     EFFSKKTTTKQI"
     gene            complement(17157..17498)
                     /locus_tag="PLK55_02905"
     CDS             complement(17157..17498)
                     /locus_tag="PLK55_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOZ35908.1"
                     /translation="MVEIRFSETAKKTISEINKDNSKLGKMISKSIAEKIELLKQDPH
                     FGEPIKSKQIPKWFLKKYNIKFLFKVRLADYWRLLYTITGTGEIEIVVVIVEFVDHEK
                     YNQIFGFKKKQ"
     gene            complement(17485..>17833)
                     /locus_tag="PLK55_02910"
     CDS             complement(17485..>17833)
                     /locus_tag="PLK55_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35909.1"
                     /translation="LNTVFGTIIIMTTKYVVKEKKTKKLKEPMIDYYPTGSEVFFIRD
                     ILEKYPEAIMKEKELEKKVKDKVSKEHLEQILKYFQHTNQIIYSKKGITYIHPATKKL
                     KDIIKKGIVYGRN"
BASE COUNT         6325 a         2519 c         2794 g         6095 t
ORIGIN      
        1 ttgtgtttgt tgaaaatcta aaaatgaact atataatagc aaacataatt tctggatttt
       61 taatgtttct tacaagatat ttctgccata ggtttttgtt taagagatat gggcactagc
      121 cattatcttt atatattctt tttcctatat atattttagt aaaacgggtg tttaattatg
      181 aatatctcaa ataacgatag aggaaagcta attaagatta caaaaaagaa tattactcat
      241 cttgggcata ttgtgaatat agcgaataac gaattaacaa taaagctaaa agaaaacggc
      301 tataatattt cgataattct tgacaaagac acaaaacttg aaatacttga agaaaaaaaa
      361 gagattggaa aaacccataa gctagatatt aaacaaaact caagccttcc ttccatttgc
      421 ctggtttcaa ccggcgggac aataggaacg catgtcgact ataagaccgg cggtgtaaat
      481 atgtcaagga ctcctgaaga gatcttgtca acagttcctg agctactaga aattgtcaat
      541 attaaaaaaa tagttcctgt tattcaaaaa gctagtgaag acatctgtta caaagattgg
      601 caagaaatag ctaaagctgt ctttgagcaa ctaaatgata aagagattga tggaataata
      661 atttctcatg gaactgacac gctttcctgg acaggcacag ctctttcctt tatgcttagt
      721 aacataaaca aaccagtgtt aatggttggt gcccagagat ccccagacag agcaagcttt
      781 gatggtgcaa tgaatcttgt ttgtgctgca cattttatta aagagaaaat tccaggagtc
      841 tacacttcaa tgcacggttc gatgagtgat gatttttgta atattataaa taccacaaaa
      901 tgcagaaaga tgcacagctc tagaagagat gcctttaagc caattaacga tacgccacta
      961 gcaagagttt atttctctgg caaaattgaa tttttaaaag acagaaaaaa atatgaattg
     1021 cttccaaaag aaaagccaaa gctagatatt tcttttagcg attcagtagc aatattaaag
     1081 acctatccaa actctaatcc agaagttatt gattggtata tctctaaagg ctataaaggt
     1141 ctaataatcg aaggcacagg tcttgggcat gttccaacaa acgacgaaac aaataaatta
     1201 gcagataaaa caaaagactg gctaccatat ctagaaaaag caacaaagaa aggaataata
     1261 gtaattatga caacgcagtg tttgtttgga agagtaaacg gaaatgttta ttctaatctt
     1321 aggaatataa caaaaagcgg tgttgaatat cttgagcagc acgatatgct accagaggtt
     1381 gtctgtataa agctaggcat agcgttagca agatttaaaa caaaagaaga tattttaaag
     1441 tatatgtcaa aaaacatttc tggggaaatt acaaaaaaag aagttccttt cgactttgat
     1501 gcattagata cttaaaataa gggaaaaaag aatggtactt aaagaatcag aaaaagttga
     1561 gttaaaaaaa tcaacttctg aattaaaaga tgcaataatt tctatttgtg caattctaaa
     1621 taaaaaccaa tccggaaaat tatattttgg cataaaagac aatggagaga ttattggcca
     1681 aaatattggg aaagatactt taagagatat tgctagtgaa atctcaagag caatagaacc
     1741 aagaatacat ccagatatta aaaaaataat tttaaataaa aaagagtgtg ttgtggtaga
     1801 atttaccgga agttcaaatt tatattttgc atacggtaga gcctatatta gagtaagtga
     1861 ccaaaatcag caattagata ttgaaaattt aaagaacaga attattagtc aaagcgatat
     1921 tatatgggat aagcagcctt caagaaataa tgttaaagaa ataaacacaa cacaacttaa
     1981 aacatttata aaaaaagcaa atcaaaatgg aaagattaat tttaaattta caaatacaaa
     2041 agacacacta ataaaattaa atttattaaa taaacagaaa ttatcaaatg caggcaatgt
     2101 attattttca aaaaccgcaa acatagaact acaatatgct gtatttaaaa ctaaagaaaa
     2161 atcagacttt atagatattg gcttaaaaaa aggcaatatt tttgaattaa tagacttttc
     2221 taaaaaatat ctttttgagc acttaaattg gagtgcggat ttaacaaaaa ctaatagaat
     2281 agaaaaatta gaaataccag aaagggcatt atttgaagca ataattaatt ccttttgtca
     2341 taaagattat acaataatta aaagtaatga aattgcgatt tacccagaca gagtagaaat
     2401 atataaccct ggcaaatttc caaaagaata caccataaaa gattatcttt cagggagagg
     2461 aaaatcaatt ttaagaaatc caagtattgc acaaatactc tattattttg attatattga
     2521 aaattgggga tctgggctta gaagaataaa cgaatattgt aaagggcaga aaataaaagt
     2581 agaatatagg ttagaaccaa acggcttatc tgtaatattt tatagaccaa aagaaggtgt
     2641 aaatgtccct gtaaatgtcc ctgtaaatgt ccctgtacaa gaaaataata gggccaattt
     2701 aatattaaat gatattttta aaactccaaa aataagaata aaagaattat caattaaatt
     2761 taatgttgct gaaaaaacaa taaaaagggc aattcaaaaa ctaaagaaag aaaaaaaaat
     2821 aaaacgcatt ggctcagata aaaatggata ttggttggta ttaaaaaaat aagtgacagt
     2881 ttatggattt agaagatata aaaaaaatag attataataa gattggttta gcctgcggtt
     2941 tagaattgca tcagcaacta gatactggta agcttttctg taaatgtaaa tctaaattaa
     3001 ttaaagacaa cacaaaacca gatagaatta taaaaagaag actaagagca gtctattcag
     3061 aagcaggaga atttgataaa actgctctta atgagcaaaa gaaagcaaag acttatgttt
     3121 attatttcta caattcctgt aattgcctag ttgaagcaga ctgccaacca ccaatcagaa
     3181 caaatcctga tgcgcttaag attgcggtga taatctcaaa attaacaaac tctcaattag
     3241 ttgataaatc aataattatg cgaaagcaag taattgatgg ttcaaatgtc tctggctttc
     3301 aaagaacagc aatgatttca acagatgggc atctagattt tgattttgga aaaattagaa
     3361 ttgacaaaat tcttctagaa gaagatgcag caagagcaat tgaaagaaaa gacacagaag
     3421 taatatattc tcttgatcgt cttggtatcc cattaataga gatggttgtt tggcacgaca
     3481 tgcacacacc aaaagacacg caaaaagtcg cattatttct tggacagcta tttagaacca
     3541 caggctttgt taaaagagga ctaggctcca ttcgtcagga cattaatgtt tcaattgctg
     3601 atggcgcaag aatagagata aaaggttgtc aagacttaca aatgattcct gagattatag
     3661 atagagagat attaagacaa ataaatcttt tagaacttaa agaagaactt aaaaaaagaa
     3721 aaataaaaga aataaggctt tctgaaatta atctaacaaa cattttcaaa aacacatctt
     3781 caaaagcagt aaaggcttct ttagaaaata aaaaagaaat gtatggcttt ttgatttctg
     3841 gcttttctgg gcttcttggt aaagttgttc aaccgaacag aagagtagga acagagctgt
     3901 caagtattct aaaagcaaag actactctta aaggcttatt ccacgcagat gagcttccta
     3961 actacgggat aacagaagca gaaattaaca aagtaaaagc agaactaaaa gtaacagaca
     4021 aggattcttt tattcttgtt atgtgtgata agaaagaaat ttctcgtgtt aaagaaattt
     4081 tagaagaaag gcttaatcaa tttatagaag gcgttccaaa agagactaga attgtaaata
     4141 tcgaaggcaa tacagaatat caaaggccac tttcagttgg tggtgcaaga atgtatccag
     4201 agactgatct tttgccaata gtctttacag aagagctatt aacagaagca aaagaggaca
     4261 tgccattgtc tgtagaagaa agaaaaaaat tatatttaga gaaatttaag ataagtaatc
     4321 agctagcaga aaagatggtt ttagataact atgcagtgac ttttgaaaaa ataattaaag
     4381 caaaccctga attaaatcca acacaagtgg cagtttttct tcttgaggac ttgacaaatc
     4441 tttcaagaga tggaaaaatc gatttagaga atgttactaa tgagggatta ttagagtttt
     4501 tctcttacaa gaattttaat aagattccaa aatcaaagtt agtggaaatt tatttaaaat
     4561 ttaataattc tgattattct ttagaagaca taatagaaaa tttaggcgtc cttaaagaat
     4621 tagataatat tgatgtagat aaaattcttt cagagattat agaagaaaac aaagaaaaga
     4681 taatagaaca aaaagaaaga gcaaaaggcc tattgatggg aagattaatg tcaaaaacta
     4741 ggggcctatt aaatggaaaa gaagcaagtg agcttattga taagaaatta gccgcctttc
     4801 ttaaaaagca ctaaaaccaa aagcttatta atataattaa tcataaatag atgtgatatt
     4861 aaatggataa tagaagacga aataaatacc ctaataacag gtatactaaa acaaatgttc
     4921 agagatcaaa aaagattact ccacatcaaa gaaaagaagt tacagaaaaa gcaaatagaa
     4981 atggttttgt tttagaaaca attcctgcaa ataattatca ggacaattct acttatcaag
     5041 aaagaaaaga aagcagaaat ttctttaagg acatgtatga caaaaacgga tattatagtt
     5101 atttatggac tccttttatt gttcttatag tcacgatatt ctttgatttt attttacaag
     5161 caattgcttt tagtttagac tcaacaatga tgttaataat taaagagagt cttttctttt
     5221 tagtattttc agtactagtc ttttgtataa atgttgcatc tattatcttt atggggttat
     5281 ctacttcaaa gcataatgtt ccatttaatg ttgcatggta taggattttt agaattattg
     5341 caataatttt cttattagaa tcggcattaa ctattattgc tttctttgta ttcccatctc
     5401 agtatattca gattacatta tttactcaat caatgaaact aacctatctg atttatctga
     5461 ttgcatggaa tatgataaaa gccgtagttt atatgattat gatgtctctt gcatacttgc
     5521 ttttctataa attaaagttt gtttaaatgc aagctttttt taaatatttt atttctaata
     5581 tattatattt ggagtatata ttatgaaaac aagaatagtt acagcagcat taccttttgt
     5641 taataatgtg ccgcatctgg gtcatcttgc aggaagtcac cttccagcag atatcttttc
     5701 tagatattca agattaaaag ggcacaatgt tttgtttatt ggtggaactg atgaaaatgg
     5761 cacagctaca gagtttgcag cccagcaaaa aggcataacc cctaaagagt tgtgtgatca
     5821 tttttatgag gttcataaaa aactttattc tgattttcta ataagctatg ataatttctc
     5881 aagaacttca agagaaattc atcataagat tgttcaggag tttttcttag atctttacaa
     5941 taataaatgt attactgaga aaataataaa gattccatat tgtaacagct gtaagaaagg
     6001 cctagcagat agatatatta caggcacttg ccctaagtgc ggttatgaca aagcaaatgg
     6061 agaccaatgt gagtcctgcg gttctgttct tgacccaata gagcttgtta ctcctttctg
     6121 ttctgtgtgt aagacaaaag acattgtttt taaagacacc aagcacttgt ttcttgatct
     6181 tagaaatcta acaaaagaca tagaatattg gatagataac aaccctcatc taagaccaca
     6241 agtaaaagct ttagcaaaag gttggataac tcaaggcata aaggaaagat gtataacaag
     6301 agatttaacc tggggcataa agattccaat atcgggttat gaagacaagg ttttctatgt
     6361 ttggtttgat aatgttatag gttatgtttc aggaactgca gaattaattg gcgaagacaa
     6421 tgctaagaag ttatggcaaa ataaagaaac agaaacatat tattttgtag ggaaagacaa
     6481 cattcctttt cacacaattt tctggccagg tcagataata ggtcaaaaaa aattggtttt
     6541 accacaaaat gtcgtaggct tacaatatct aaattttgaa aaacagaagt tttcaaaaag
     6601 taaaggtgtt ggtattttct tagataaagc aataaacagt ggcattccaa tagaatattg
     6661 gaggttctat ttaacatata ttttaccaga aacaaaagat tcagagttta tgattgatga
     6721 gttttttgat agaataaaca aagaccttat aggtaattat ggtaattttg taaatagagt
     6781 tttaaatttc atttatacaa agagacaagg aaaagttcca gaaataaaag aaaaagatgc
     6841 aaattttgaa aagcagattt tagagtctat agacaaaatt gaaaaacact atgacgcttg
     6901 tgattttaga tatgctcttt atgagttatt aaaactttca gatcttggaa acaaatatct
     6961 taatgagaaa gaaccgtgga agacagaaga tacaaactct ctagcttatg gttttgagat
     7021 aataagatta ctttcaattt atttttctcc aattcttcca gagtcttcaa agaagataag
     7081 tagtttttta aacacaaaca atcaaaatat agaaatagat actaaagaaa aaaaaataaa
     7141 taaaccagaa atattgttta gaaagctcga agatacagag aaggaaaaat taatgtagat
     7201 attgtgataa tatgatagta aaagaaaaat gcaaatctct ttatgcacct tggagacaag
     7261 aatatgtttc taaagtaact gatgaaaaag aaagcacaaa agataaagag gtctgtcctt
     7321 tctgttctaa aaaccttgat aagaaatatt tagttcttga aacaaagaaa tcttttattc
     7381 aaacaaataa atacccttat gctgctgggc atttacttgt tgttccaaaa aggcacataa
     7441 atcaagtcat agatttaata agtattgaaa gaaaagaact atttaatctt atcgatcttt
     7501 cggtttttat gcttgataaa tatatgcgcc cagaaggcta taatgtaggt tgtagtattg
     7561 ggcgaattgc aggcgaaagt atacagcatc ttcatttcca tattcttcca agatataagg
     7621 gagatgtagg gtggaatagg ctttgtgatt ttaatgttat atctatttct ccaatagatt
     7681 tagcagttga tttgagaaaa ataattcaga aagaaaaatt agcgaaaaag tttggtttat
     7741 agttgagttt ttagctataa agtcaatctc tgccggtttc ttcagctaat ttcagatttt
     7801 gctaaattac agataataat ttaaatacta taggcacaat atatatactt tttaataaat
     7861 ttaagaatat aacgggaatt tggtgaaaaa atatgatgac agtcttaaaa aggcttttta
     7921 gaaagaagac aagcgcaggc gaagttgaaa ttgatgaaac aatagtaaaa gaaacaaatc
     7981 agttatcaag tactgcagta ataaaaaggt gcgatttcag atcaaaagat attaattggt
     8041 tagaagggca cattcaagca gtagacgatg ttgttaataa aaacagcctt ggtgcaaaaa
     8101 caaagaatca tttcgaaata gatggtagaa gatatttcaa agacgatacc actagagtat
     8161 tgtttaatag attatatgaa aattatacag catactatta tactttaaca gaataccttt
     8221 cgttaagaaa agaattatta aagtataatg gtttaggctc tgaaagtttt tttgttgcag
     8281 gccagataaa agacacagaa gagcaaatgg tggcaataga aaaaaagcta aatatctatc
     8341 ttgataatat aacaaaaata aagcaaagaa taagacaata tgttggatta taaattcaac
     8401 attttccttt ctattttttt aataataata tttaaaaact cttttattat taaatatatt
     8461 agaaggttgt aaaagaaatg gaaacgtttt ttcaagttgt aatcctattt attttagtgg
     8521 ttctttctgc atttttttct gcagcagaaa ctgcattaat gtctatacat tttgttagag
     8581 ttaagtctct ttttaagaaa ggtaaagctg gttcaaaaac tctttataat cttaaaaaag
     8641 atcctcacaa gcttatagtt acaatactta ttggaaataa catagttaat atttctgcat
     8701 cagccattgc aaccagttta tttctaagtc ttttcggttc agaaggcgta gcaattgctg
     8761 ctggagttat gactcttata attttaactt ttggggagat tcttccaaag acttttgcaa
     8821 tacagaatgc agagaagata tctttacttg ttgctgttcc aattcattat ctgtctattc
     8881 tattctatcc attgattatt ttttattcat caattactaa attagtaagt agatgggtaa
     8941 gaccaaaagt tgaaaagaaa atctcagaag aagaattaaa gataatttta acattaagta
     9001 gagaagaagg gatacttagt agaggcgcag caaagattat gcataaggtt ctagactttg
     9061 aagacacgcc agtaataaaa gtaatgactc caaaagagca gatgaaaacc attgatggag
     9121 atttaaagat tgatgaagca atagatgtaa tttcaagata tgggcattca agatatccag
     9181 tttatttcga agatgaagat aacataattg gtattgtaga tgttgatgat gttttaagag
     9241 caataaagga aaagaaaagc aaagaattga taaaagatat tattagacca atagagtttg
     9301 tttctcctga aaaagaaatc ggagatattt tctatgattt tgaagacaaa tctgttttaa
     9361 tggcaattgt tgttgataag tctggcgaag taataggttt attaacaatt gaagatattc
     9421 ttgaagaaat tgttggtaat atctttgaca aaagcaatag aaaaaaaaga agaaactaat
     9481 taaaccatgc tggtttcttt ctttttgtcc atttagcaaa atgtgctttt tcgtttttat
     9541 agaattctct gtatgctttg actgcatctt tttgtttata ttcttcagga attgcttgtg
     9601 caaattcagt taatccaata gacaacaaac tttttggtgg tgtttcaagt atctttttta
     9661 tttctgtggt tttgtggtgt ttacaatatc tatattcata ttctttaatc agttcagaaa
     9721 gatgctctaa aagccaaaga tagttttctt tagattttcc tgcccagata acacaaggat
     9781 gtttctcgtg tgtcttctta taaactagat gtttatgtgg ggaatcataa agatgatgaa
     9841 ctgttgaaag aatttgtgcg gattcaacaa tcatcttaac aacatgttta tcgcaaagca
     9901 ttcttgctgc tttgttagga tttttatcaa taacaaatat ttgcataatt tagaccatgt
     9961 ctcttgccat gcctttaacg gatcttaaac actttttgtg ccagtattgc ccgccccatt
    10021 tcttgtcgca gcccttctgg ctacacacag cacaagtttg atcagggtat ttagaattag
    10081 cgatatcaat ttgtgcagca ggttttgctt ttaacttttg cagttctgct tcttttgctt
    10141 tttcttcttc tgttttttct tttttcttaa atgcgtcaaa tattcccata gtattttctc
    10201 ccaaatcaca ataatagccc ggaggagatt tgaactcctg tcacaagatc cagagtcttg
    10261 tatgcttggc cactacacta ccaggcttta ataatataat tagatataaa acaagtttaa
    10321 aactttgtct tttttataat ttcataaact tctttccaat ctcttgcttt ttttatagat
    10381 ttaggaaact ttccttttga ccaagcatag ttgtctttat aatcaagaat aattgctttt
    10441 actccgtgat tataacaatc caaagcataa tcaattctat catcaacaat taaagagatt
    10501 ccaagttctt tacaaatctc tccttttgtt ttcttgaagt ctccttcttt tctatattgc
    10561 ccaaatatta tttttttaaa catttttggg aaatgatgag aaatccaatc tcttgtgtgc
    10621 tttttaacat ttttggttct tgcagtcaca agatataatt caaattcttt tgcaagtttt
    10681 ttaattgcta aaacagaacc aggcattggc ttaaattcag aaatatcttg cttgtttatt
    10741 gtttttctta tatatctata aagtgccatt tcgtcttcaa aagaaaaatc cttaatagaa
    10801 tgataccctc taaaagccgg aaagctcttt tctttaagca tttcttttat caaaagatcc
    10861 acagtaggaa caagagtgtc atctaagtct atagcaattt ttattttttt catgtatgga
    10921 tttcactgcc acataatttt tgcaagtttt ccagagtatt tatatttatc aagaagtgga
    10981 gagttcttaa tctttgcatc ttgatgtttc tgtaaagtgc ttgagatttt ctctgccaag
    11041 tatttcttgc attctccgca aagaatcttt ccagacttac agtctttttt tctttgttcg
    11101 agcttttcgt cgcagtcttc aaataagatt tctaaccaag aataaacaac acaaacatca
    11161 gtgttgccgc ctttttttct ttgctcttca agggttggtt gtccacctga gaaagcatat
    11221 ttgtttattt ttttttcaat agtctttaaa tcatcattta aaaagattgc agtctcttca
    11281 acagaacttg acatctttcc gtctattcct tgtaaaggtg gcaaaagctt attgtgcaca
    11341 gccactactt tcttgtaacc gagcttttct gcattatctc tttgtattct ccagtaagga
    11401 tcttgatcta ttcctgcagg aatcaagcaa tgtttctttt caaaggttgt tggaacaact
    11461 tgaatagcag gatagaagaa ttgtccaagg ttattgtcat tattaaatcc aaacactgct
    11521 ttagctgttg aaaaggttgt tctttttgca aactgcaaag ccacaggata aagtttttgg
    11581 atatactcca tatcagagaa gaaaaaagtc ttgtcggtat taaagccaac tgctgagata
    11641 tctaaaaaat tgtctttctt gtttttttcc atttcttcaa ttgttaactt atccttaaat
    11701 acgaactttt catcatctgc catagcaata taaacattac aatcaaattc gtcttgaaac
    11761 catttagaga taataaatgg aataaggtgt ccaatatgca tctttcctga tggccctcta
    11821 ccagtataga taaaaaagtt ttgcttgtct atgtcttcta atatcttatc aaagtctcta
    11881 tgtgaataga taaatcctct tctaaagaaa ggatgtagtt tcttggattt ggtttttaac
    11941 cttaattttt caagcatttg tttatttagc tcgtttgttc caaactgttt aatcaatttc
    12001 tgataatcaa ctgctccttc aatttcccaa ggcgttactt tgaattcttt ttctgacatg
    12061 atttattcac tttaagtata aatattatat tataactaat aatagaaaca tattaaaaac
    12121 tatatctggg ctacttgcaa agcccataat gcttaagagt gttatatatc ttatttgtgc
    12181 tcttaagata gttttcttta ctaaattctt ttgtgtcttt aaaaagaaga tgtagcttgt
    12241 ttgctatttc tctaagcata aagattcctt tagaaaacac aaagaaaaag aaaacatctt
    12301 ttgggacttc ttcaagttta ttgtttttac ctaagatata tcctttttga gaaccagagt
    12361 acatcaaaaa aagattatta attttgggta gcgcacttat ataatgtgaa ttatgatttt
    12421 ttttagaatc tatataatca ataagcattt ctttttctaa aagtgatata aaattataaa
    12481 atattttttc tatattatct ttctttgttt ctgggtgtag tctattaaga taataaataa
    12541 tttcttcttt tgtaaaaaat ccgtcaagga aatgtacaat atcaatataa aactcaagat
    12601 attttttttc agttttaaaa agaaaagaaa ttatttgctc aaatgttgtt ttgtctttta
    12661 ttttaggtgt ttcttttagt ctttctttaa gttcttcttt cttttcaaga gcacaaagta
    12721 aagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttatct ttaattgtaa
    12841 atagagtttt tcaagtccat tttctgctaa aagctttgcg tcttcaaaag aaagggcttc
    12901 aaaatcacaa gtcaaataat aattcaaatc aaaatagtct ttttttagtt ttagtatttc
    12961 ctttagtgca gaagaacggt aattataaaa ccagaaatca tagatttctt gggttaaaat
    13021 attttttatt tctggtaaat ccattaaatc ttggacaaca ataaactttt gctcctttct
    13081 ctgcgtatat ttttgaatat ctgttatttt ctgcaagttt ttttctttaa atttttcgtc
    13141 tctgcaagtt tttttcttta aggcttcaat cttgatattt gggtaaactg tttttatttt
    13201 ttcaatgatg ttttgaaaat tttgattata gttctcaaag tatttcccct ctaagtaatc
    13261 aaaaattatt ttttgcggtt tttgtttcat tatcaattcc agacaagatg ctagagtttt
    13321 tggtttttta gaagaaaaaa gctcaaagtc aagggacaag aaagaaaaag aattaagaga
    13381 aaacggaaaa tatggcataa gcagaggggg ttttggcgaa ttgttgttag gttctttgtc
    13441 aatattttcg tcttccgcag caattagcgg aaagtagggg ggtattacaa ggattgtttt
    13501 catagtagaa aacttttggc ttgaattaaa tattttgtga atttgaagct ttatatatgt
    13561 ttctcacaaa atttttgtga aaacgcaata tttatatgca tttttctcat aaatacaaat
    13621 atgcaggaaa tagacaaatt tgacatgcgt tttttagtag agttagagaa agattccagg
    13681 cagccaatct ctaatatcgc aaagcttctt aaaacctcac agcaagtctt aagttatcgc
    13741 ctaattaatt tagaaaaacg tcatataatt cttggttttt acacagttat taattttgtt
    13801 aaattaggtt ataccagcta caggacaatg ataagattaa caaatatttc tccagaaatt
    13861 caagcaaggc taatttcttt tataaaagaa gacaacaatg tatcttatct tgcagaatgt
    13921 acaggacact gggatttgat tgttgattat cttgcaaaga acattgttca atattattct
    13981 ttcttaaaag acataagaga aaagtttccg gagaatattg gaagcataga tgttctaaca
    14041 actcttgaaa acatatattt tggcagagat tattttgcaa agaaatacag agaaataaac
    14101 aagaaatcat ttattgacac agattatcta accacagaaa aattagataa tttagattta
    14161 aaagtacttt atgaaatatc taaaaatgca agagtcggtg ctatggagct tgcaaataaa
    14221 gttaagactt ctccaaacac cataatctac agaataaaga gcatgcagga tagaaacata
    14281 atcgagggct ttaaaccata tctaaatatt gagaagaccg gaagatttgc ttacaagtct
    14341 ttaataaagt tcaataacat taatgagcaa gcagaagagc tgttgattac ttttcttcag
    14401 aataatctgt gtgtctttga tatcacaaag gttattggtt attgggattt tgagattgac
    14461 tttgaagtta aaaactcaga tgagatgatt gattttacaa gaacagtaag agagaatttt
    14521 aaagatttaa taaaagagtt tgaaacaacc ccaatctata aagaacacat tttcaattat
    14581 ttccctggag atcttctgtc tcctgagaag aaaagtaaga agaaataatt attctgtttt
    14641 ttctttcttt gggaagattt ttattgtaat gctaaatatc attcttgcta caaattctat
    14701 aattacaatg aacacaaaat aatatactaa caatgataat ttagaaggta tgtcttttaa
    14761 taattctata actgtaggca tagagaaata agaaacattt actaaaaata ttccaagaag
    14821 tgtaaatgga agcatctttg ctaaatctct agataagtct tcgtcgtgat atgctgtaat
    14881 tcttattgct ccaattaatg caatagcagt taacaatatt gtttcaatgg ttagtccttc
    14941 tgctaagaaa gaaaggatta ttgttaatac tacaaaacta aagaaaatta taattggtgt
    15001 aaaaaagaga tactttataa catataaaat attatagaat agttttttaa aaaaagaatt
    15061 tttcgcatct ttgtattttt ctaaacttat agaaacaatg tctcttttgg caataaatct
    15121 atagaattta aaaataaata tagagtaaat aaccattcct ataacaaaga aagcaagtgg
    15181 ttttaatatt tctatagcgt ccgcaaaatt tattgaaatc agagatatat aaaacgcaat
    15241 tatatttata tttgttttgt tgtagttttt ttactaaaaa attcttctat ctctatttta
    15301 aggtctttac taataacaaa ctgcatatcc tttgggaaac attttctata gttctcccaa
    15361 tggaatctcg catcaataaa aacaattact cctttgtctg tgtctgttct tatgactctt
    15421 ccagcgctct gaattacttt ttgtattgca ggatgaatat aagcatagtt ccagccattt
    15481 ctaaacttaa tctcataata gtcaatcttt gcttttgtct cgatctccat ctttgcaaga
    15541 ggtattccaa caatcactgc gccgataaga agattaccag gaagatcaat tccttcagaa
    15601 gcttttcccc ccattacagc cattagtacg cttccaaact ttttagagtg tttcttaaac
    15661 ccaagaatca tttcttcaaa gtccttgcta tccattcttt cttcttgaac aatagttggt
    15721 ttattacaat taattaatgg aataatatct cctaaaactt caaagcttgg aaagaaaaca
    15781 acacagttac caggaattgc atttacacac ttctcaacgt ggcttgcaat ctttttatac
    15841 tctaaattgt ttctttgcgt gtattttgta gaaacatcag taatcaaaag atttagtctt
    15901 ttttcttttt caaatggcga agaatattct tttgttgttg tttcttcttt tttcaatcct
    15961 aagatatctg tatacattga aagcggagta agtgttgctg acatcaagat tgaagaatag
    16021 cagttttcaa atattttctt agttagtgga gagacatcta aagcattata cttaacacta
    16081 atcccgttct tttcttttct tataaaccgg atgtgcgcat ctttttcttt tatccattct
    16141 tcaaggaata accctacaga gataagagca gagctgcttt cttttaggtc ttcaatatac
    16201 ttaagtcctg attcatcaag ttcttcaata actgaaataa cgtcttctct aatttctttt
    16261 atcaagattt cttctttttc gattctatct tcttttattt tttctagttt tttttctgca
    16321 attctttcga tgtggtctag ttgtttttca ataaggtttt taagtttgtt attcttgatt
    16381 tcttttgctt cttttattgc tcgatgaagc gtatttgtgt ttaaggattt agaaatcagt
    16441 ttcaaaagtc tgtcctcaag gttatgcgct tcgtcaataa caattataca ttcttctaag
    16501 ttgactccaa tctccgaaag tgttgattcc ataaccttgc ttgagaaaac atgatagtag
    16561 tctgcaataa taactctgca gcttttagca aaaagttttg caagctcata tgcacaaaca
    16621 ggctgtcttt ctcctgttgc gctctttagt tcttctgcta tctttttaat ttcaagatgt
    16681 gttaagactt ctttttctga tagcttcttt tctaaaagat aagctaataa gtctctttgt
    16741 cctttgttac ttggtctaat attttcaaaa aaagggcatt gttttttttt gcacgccata
    16801 agacagattt cataaaagtt agaatcggtt cttgagatga ctggctcgat gcacatgttt
    16861 ttctttccaa caaagtcaat ggctttaatc tttaagtcat atttatcgtt aagatcatca
    16921 ataacttcta aagcaatctt atgttgagat atctttggag tcaagaaaag aactttcaag
    16981 tccttctcaa tagccaaggt gattgctgca gaaagagcag agtctgtttt tccagtccct
    17041 gttggtgcat gcacaagtat gtttttccct aaggaaatac tctcatagat atccctaatg
    17101 atatcctttt gtccttttct aaaagaatca tgtctaaaat aaagttgcgc tttcatttat
    17161 tgtttcttct taaatccaaa gatttggttg tatttttcgt ggtcaacaaa ttctacaatc
    17221 acaacaacaa tttcaatttc gccagttcct gtaattgtat ataaaagtct ccaatagtct
    17281 gctagtctta ctttaaataa aaatttaata ttgtattttt ttaaaaacca ttttggtatt
    17341 tgtttagatt ttattggttc tccaaaatga gggtcttgtt taagcaattc tattttttca
    17401 gcaatagatt tactaatcat ttttccaagt ttactattat ctttatttat ctctgaaatt
    17461 gtctttttgg cagtttcaga aaatctaatt tctaccatac acaattcctt ttttgataat
    17521 gtcttttagt ttttttgttg cgggatgtat ataagttatt ccttttttag aatatattat
    17581 ttggtttgtg tgttgaaaat attttaatat ttgttctaaa tgttccttgc tcactttatc
    17641 cttaaccttt ttttcaagtt ctttttcttt catgattgct tcaggatatt tttcaagtat
    17701 gtctcttata aagaacactt cgctaccagt agggtaataa tcaatcattg gttctttaag
    17761 tttttttgtt ttcttttctt taacaacata ttttgtagtc ataataataa tagtaccaaa
    17821 aacagtattt aag
//
LOCUS       DAOOON010000016        16569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_4254_length_16569_cov_22.229199, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000016 DAOOON010000000
VERSION     DAOOON010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_16569_cov_22.229199"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLK55_02915"
     CDS             <1..783
                     /locus_tag="PLK55_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35910.1"
                     /translation="RITLNHNANLILTQKMYNSSEDLKFYTLYRNTKVDSTIPVTITS
                     MDKYTFSYNDSENKNITGGNTMKGIVGTRENPIRSYDGYSQIPYLTNNPTVYSYSDAQ
                     WYINQNSGNKFSEKTINPSQFPYPEANYFDFDCQGYNLNFISSEVEKIIYEIDLDYYS
                     GTQYVIVNNIENPYTTTNDSGNFSAEIINQLNDGHDHAVKIDAYLITTENSGQLITNV
                     SKIRHLNCQTGTSENVKQSNEITEEELNSKIRILEEKGIFNK"
     gene            complement(795..>1184)
                     /locus_tag="PLK55_02920"
     CDS             complement(795..>1184)
                     /locus_tag="PLK55_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOZ35911.1"
                     /translation="EIDKDCKEPIITILPGQENKRIGGSMRLGSCVAELQENSQVAKI
                     YGKNKITERHRHRFEVNPKYHSILKEKGLGLSGLDPTGELLEFIEIKNHPFFIGTQAH
                     PELKSKLLTPHPLFVEFVKKAKEKKEK"
     assembly_gap    1185..1284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1285..2313)
                     /locus_tag="PLK55_02925"
     CDS             complement(<1285..2313)
                     /locus_tag="PLK55_02925"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOZ35912.1"
                     /translation="MDYKDISGIYDLAKEKKLKLVVVTGGVLSGLGKGVSASSVALLL
                     DKNLKKIPIKCDGYLNFDPGTMNPIEHGEVFVLDDGTEVDMDFGHYERYLDISCNGTQ
                     NLTMGKIYKEIIDLERSGMFLGRTVKLIPEVTDYIEYRILKTALDNKAELVLLEIGGT
                     VGDLENELYIAAAKGIKNKLGKENICFIHLAYVPEIAPSSEQKTKPAQNSVSELFKRG
                     ITPDALLCRSKRILDKKSIDKLCDNCGLLPEQIISANDVKNIYEIPINFKNQNLDTLI
                     SKKLNIETNKEFDKFIEAVTKIENNNSEKIKIGICGKYTGIDDSYASVIEALHHAKTT
                     VPESLEIRL"
     gene            2635..2717
                     /locus_tag="PLK55_02930"
     tRNA            2635..2717
                     /locus_tag="PLK55_02930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2669..2671,aa:Ser,seq:tga)
     gene            2668..3150
                     /gene="metG"
                     /locus_tag="PLK55_02935"
     CDS             2668..3150
                     /gene="metG"
                     /locus_tag="PLK55_02935"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOZ35913.1"
                     /translation="MRAGVERLREFKSRPLRIPFFVKKAISFGKKHKIYSKKKQLKTN
                     DLIIMSEEKPIITFDEFSKLDIRVGTIIEAEKVPNSIKLIRLVIDFGDFKRQILAGIA
                     TKYSPEDLQGKQIPVVVNLAPRKMAGQVSQGMILAIGDGEVEALLQPTEKVKEGGSVH
                     "
     gene            3160..3429
                     /locus_tag="PLK55_02940"
     CDS             3160..3429
                     /locus_tag="PLK55_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOZ35914.1"
                     /translation="MDKVKVNYFIDVPLALSFIIVAITSLILFFFLPSGVRQAGYQMF
                     WGLTKHTWSQIHTISGFIMIILSLVHLILHFKWLTTMSKTIFKKK"
     gene            3504..3586
                     /locus_tag="PLK55_02945"
     tRNA            3504..3586
                     /locus_tag="PLK55_02945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Ser,seq:gga)
     gene            complement(3592..4650)
                     /locus_tag="PLK55_02950"
     CDS             complement(3592..4650)
                     /locus_tag="PLK55_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOZ35915.1"
                     /translation="MLEQVIFGNTIKNYLWFLFFIALGFVVGWVMVFIVKNIFIKLTK
                     YTRNKIDDIIVKILSKPMPIKLIILIIFFNIGFKYLKSTTGLQNFINKATFIFLVFAI
                     TLFLIKFFIGLIEVYFEPRAKKSESKYDDQIIPVIKSLIKIILFTFAFLLVLSNLGYN
                     VTALLAGLGIGGIAIAFAAKDILENLISGVTIFIEKPFKVGDALKTSEGLGTVEEIGI
                     RSTKVRTFDNTIVVIPNRLLSTNSVENISARRARRENYLLTLVYGTAEKKIQKAQEII
                     KNTLTKNKDIEHDSIVIGFESFGDFSLNIRVVYWIKAAEYDQYMAIKNKINSTIKKEF
                     EKENIEFAYPTQTIEIKK"
     gene            4798..5103
                     /locus_tag="PLK55_02955"
     CDS             4798..5103
                     /locus_tag="PLK55_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HOZ35916.1"
                     /translation="MGILKEFKEFLQEYKVIGLAIAFVIGAAITALVKSLVDNIIMPI
                     IAPLIPGGSWRTAILTLGPFKIGWGAFLGEFLNFVIIAFVIFMIAKMILKEDKVAKK"
     gene            5119..5349
                     /locus_tag="PLK55_02960"
     CDS             5119..5349
                     /locus_tag="PLK55_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOZ35917.1"
                     /translation="MERQPVDDKDSRMIGNLVSIGYDSDNQVLEVEYKLGYIYQYHKV
                     PKDVYERLISERNKIEFVQQRIAKVYRNQRLK"
     gene            5466..6197
                     /locus_tag="PLK55_02965"
     CDS             5466..6197
                     /locus_tag="PLK55_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOZ35918.1"
                     /translation="MPQDIIFKLQEIGLSKNESTIYLELLSNGEASANELSKKINFDR
                     TLCYQLLNKLIEKGLVHCYIKKSKKIFNITDTKDLLLSIRKKEQIAEELIDKLNLLKT
                     KSSHEEQNLLIYQGDKGIDAVFTELLKAKEVYIFGGAAKTFNKKKMNEKLISKLNKLK
                     IKGKAIISDPKSYSELGFNIKELMVRELKGMNNTVTTIIYGNKIGIHILADNPIVIFI
                     DNKTVAKSYLEHFKNLWNQAKPLTI"
     gene            6211..7389
                     /locus_tag="PLK55_02970"
     CDS             6211..7389
                     /locus_tag="PLK55_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOZ35919.1"
                     /translation="MITLQNLIIKIAELQKKHNYYILIAVVLITVFMVIGATRLEMQG
                     DLSKEMPQELPIFKLNERISDKFGGQDIILVVLEVENNSNSKSAVNDLRDPRVITYLK
                     DLEASLKQTTTIENAYSVGTFLQGYNFEDTTQVSNFLSRSPQTLDFYSKDYKLTAMYV
                     TTDVGSGQDKIIATTNLITEKINSLSMPPGIKVTVTGNAPMSNTLLDILKKDAIFTLL
                     LAALIILIILMITEKSIPKGLVVFTPLFFGIIWTIGTMGWLNIKLSVATVGIGAMILG
                     LGVEYGVFMLSRYKEERDKGKTQLETLKIAVPSVGISIMGSATTTIVGFLALTSSVMP
                     MLQHLGISLALGIFYCFLAALFIEPVIIIKMEEWEYHLANKLHERFAKMKASAKGDLD
                     "
     gene            7386..8525
                     /locus_tag="PLK55_02975"
     CDS             7386..8525
                     /locus_tag="PLK55_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOZ35920.1"
                     /translation="MSLINKIVEAYSKFVSRNLWLMVIIVLIITGISAYFSSTITTKN
                     MDYRDMLPEDRDTIHALFAIEEGLGGTETAMIAIELDSQYANSNESRDIRELEILEYI
                     DKLSKLSDEIEDVESVSSITTIMRASNNRELPKSKTEINNFISNNPQSNQYVSSDYQM
                     ALIRLRLRDDFDDKQILKEIEDTISLIPKEEGVVVSPAGQALTSAAVNNEIGGDIAKT
                     GQLSMIGIIIILLLLFRSIIFGLTPLSTILIGVVWAFGYLGLTGIGISSITSGAISMI
                     MGVGIDFGIQIVNRFKEELQKTKEEAKAMEITLNNVIIPMGTTTISALIGFTAMSMGE
                     LTVMKDLGSILSYGIAACFLASISIVPVLLILILRFYKKIKERKK"
     gene            8522..9778
                     /locus_tag="PLK55_02980"
     CDS             8522..9778
                     /locus_tag="PLK55_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35921.1"
                     /translation="MNKKYSFIVYASLLLLIGLLIKPIYAADDLDTASLKVTLLNQDP
                     DPAEPGEYVDIRFKVEKFGNNPLTNISFVLEPEYPFLFDDSDVPERNIGTIKYASGDD
                     WYYNLKYKLKVDSEALEGTYDLKLKYEVNNSNVKNIVEFNIAVGDATKPDFVAGNIIS
                     SPTKLVPDTDSAQLNIEISNIGDEDAQNVVAKLNLPTELIPTYSYSDRSNLATIASGT
                     SKVAIFYLDVDKIAESKIYSVPLIITYKEKNDNTNEYKTKTINIEIPIKEKPVFKVTQ
                     IITEPVNAAPGDKVKIIINLQNTGKDAEAVSLKLYKESSQPITFDEKTDYIGTLDKGE
                     TGTGIISLEIDKDAELKDYLLDLEIRAIDGEQVIVQNETISLKVSKSNKQLSIIWMVF
                     GIVILVSAAAGFYFYNKSKEDKKKKK"
     assembly_gap    9921..10020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10021..10664
                     /locus_tag="PLK55_02985"
     CDS             <10021..10664
                     /locus_tag="PLK55_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOZ35922.1"
                     /translation="KSFSSKESLGKERGVFMNYLYLDIETCPIDKELYDSLEDEEAKL
                     KLLNPIDSRVVAIGLKYSDSKDTIILQDDNEKKILEDMWQTISKLRGNGTSLRIVGFN
                     ILNFDMPFIIARCFINKVSVLPISGNEIIDLRDLVSFFRTGKTRGGLKDFGKAMGIEL
                     DENDGGDIARLCFQEDKEDGKKQIAKYLRKDIELTESMHKQIMSLGIDKLRRY"
     gene            10735..13254
                     /locus_tag="PLK55_02990"
     CDS             10735..13254
                     /locus_tag="PLK55_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGF-pre-PGF domain-containing protein"
                     /protein_id="HOZ35923.1"
                     /translation="MKLTKILIFVFILLFMQNLFAFNPTFYVGDGVSGTDPNCQNPNY
                     SSIQAAANSIKNDAIQNAIIIVCQGTYTENINLTDFENLSIIGENDEVIIKSPATEVS
                     PLQVFFSTNIGLKNLKIIEPNLKGVVLNNVIDVILEDISVSEIKGIAQGEYSLAGIYI
                     SGSDNVTLKGDIKIEDVDASIKYTNGIEIRNSEGIVFDTDLLVVYNIKSSNNATDSLL
                     TGILINNSDIEIKSNVTISELNGRKDVIGFLAENDSVVTNENEINMGVTQSRSLSVIK
                     GFAIENSRINLNDNTRTNFFSIGGFQADTYAFYLDNAQVSWLGELEFIRVIGEDKSYG
                     LYINNSDNFIVSKIDTDNTQIEGEVRLIYIKDSDSGQIKNITNGNEEIMITTNDFRVL
                     NIINSSVKVNKMRITSNRDLPNCIVAENNSNIELEDIDLTNCSGNYIKNNNSEISIKN
                     SPTINLAKIQYDQSQNSIVEVYKPVKLLPYKDGRDLKLDELYIYHDSKYFSNIFNPET
                     EIEYDLLYFTKGYKNGVLDSSFYNDYYFTGIKSRVNEKKEKITINDFSFQEIYIEFFI
                     VPNEFLEQTAKAGVTTKNSDHFSVTDKTCSKTLDKSYGCFDIKLSLALEDTENESYTL
                     KFKLPVSWLTQKNLKKDEIVLYHLENNKYVEQKTTPLTIVDNYQIFISEVDSFSEFQI
                     AEKKDVSDVDVPKNDTPPETTPPVTEPPADSNQTNPNCNIKCTENQILDYINCKCIDK
                     QTGVCDLVCDSNEYLDTELCQCFDLANAGCRGACNVGETMNLTTCTCVPVVLDGMPIS
                     QANNFWSFVLIVLVIVIAIIVFLIIRKNRKNKLKKQSKKKK"
     gene            13342..15963
                     /locus_tag="PLK55_02995"
     CDS             13342..15963
                     /locus_tag="PLK55_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOZ35924.1"
                     /translation="MAIEKTKSDGTDPFEKIEKGKNDKSIYESVPEEEKADEKKTKTT
                     AREPERNRLKEITPKLEFLTDPEDETIVYIGRKKSVLKEHGSKSCAFLGKVYEDDCRD
                     KKVYLDGLNPHVVFIDGSRGSGKSYGLGVIAEELTTNNPYIGQIVVDPVGVFWSMKYP
                     NQEKKELEELKKWDLQAKGLNNIKVFVPEGVVGKVSKETYDETFSMYPSMLLADDWCL
                     TFGIDRFNPTGLLLEKTLSFVKHGYKSGDPSEQEKTTEQIPAKGNLYNLDDIVFCLQN
                     AYEFKSSSSGYKQDSIRALVSRFEAAKSWGIFSDHGTPLSKLSKEKQLTILDTSFLDD
                     NITALVIGILARRILSARKLVTRQDAIKKFDSNSESPDELLEISIPPTWVYIDEAHTL
                     IPSGNTITPATNAIIEYVKQGRRPGCSMVLATQQPSAINSKVLSQVDIFLSHKLVFND
                     DIHAVQKRMPAIIPEAYKDPNFIKTLPVGVALTGDRGEETKRAFILKIKPRLSQHEGR
                     DAETIREVPTLSKKEIINFCSDMIYKKLLSSKTLKTSDAKDILDVVNSKYNSDVVFDE
                     ILVSLSKMGISEKDNILYFENKNTKQKTEIPKDEIQDINRAIEKSNELSEISEDMPLT
                     PEEIVPDSLPVIPSRISRDIAINIANSTNKKKFLFLREEGIISQVTMIYRKVYKINFN
                     IFDEDENYKTSTCFIDSVSGEFIHFDGRNFKASNGFKLVEELSVDDIVLLDSIFAWKT
                     PEELSEILGFDAREIKQDLISLVDRNLADVDIINKKESFRIKRRIDLPFHPGYDILTS
                     INHLPLKKQLISKSDIQEEISEEIIVSRLEKLWPDIEITEISEIFWPLWEIISKDKRA
                     NQRAIIVDAVIGQIIDA"
     gene            15977..16312
                     /locus_tag="PLK55_03000"
     CDS             15977..16312
                     /locus_tag="PLK55_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35925.1"
                     /translation="MLDLLKKKEEKSLSIDSFILDSCKATPGFEFFPKKPIKIDLEKL
                     LMKLRTKNYYIEKDNSPFLIILKTKKGTVTIFSSLKIIIRDTTQEKVAKVILTDLLKT
                     INEFLYKQG"
     gene            16303..16391
                     /locus_tag="PLK55_03005"
     tRNA            16303..16391
                     /locus_tag="PLK55_03005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16339..16341,aa:Ser,seq:cga)
BASE COUNT         6108 a         2401 c         2668 g         5192 t
ORIGIN      
        1 agaattacat taaatcataa tgcaaattta atattaactc aaaagatgta caattcttct
       61 gaagatctaa aattttatac attgtataga aatacaaagg tagattcaac aatacctgta
      121 acaataacct ctatggacaa atatactttt tcatataatg attcagaaaa taaaaatatt
      181 actggcggaa acacaatgaa agggatagtt ggtacaagag agaatccaat aagatcttat
      241 gatggttatt ctcaaattcc ttatttaaca aacaatccaa cagtgtattc ttatagtgat
      301 gcacaatggt atataaatca aaatagtgga aataaattct ctgaaaagac aataaatcct
      361 tcacaatttc catacccaga agcaaattat tttgattttg attgccaagg atataatcta
      421 aattttatat catcagaagt agaaaaaata atttatgaaa tagatttaga ttattatagt
      481 ggaacccaat atgtaattgt aaacaatatt gaaaacccct atacaacaac aaacgactca
      541 ggaaattttt ctgcagaaat aattaatcaa ttaaatgatg gtcacgatca tgctgtaaaa
      601 attgatgcat atttgataac tactgaaaat tcagggcaat taatcactaa tgtttctaaa
      661 ataagacatt taaattgtca aaccggaaca tctgagaatg ttaaacaatc aaatgaaata
      721 acagaagaag aattgaatag caaaataaga atattagaag aaaaaggcat ttttaataaa
      781 taatctataa aagattattt ctcttttttt tctttagctt tttttacaaa ttctacaaac
      841 aacggatgtg gagtaagtag ctttgattta agttcaggat gtgcttgtgt tccaataaag
      901 aaagggtggt tcttaatttc aataaactca agaagttctc cagtaggatc aaggcccgaa
      961 agaccaagtc ctttttcttt aagaatagaa tgatacttag gattaacttc aaacctgtgt
     1021 ctatgtcttt cagttatttt attttttcca tagatttttg caacttgtga attttcttgt
     1081 aattcagcaa cacaagaccc aagcctcatg cttccaccaa ttcttttgtt ttcttggcca
     1141 ggcagtattg ttattattgg ttctttacaa tctttatcaa tttcnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnaagtct tatttctaga gattcaggaa cagtagtttt
     1321 agcatgatgc agcgcttcaa ttactgaagc ataagagtca tcaattcctg tgtattttcc
     1381 gcaaataccg atctttatct tttctgaatt attattttct attttagtta cagcttcaat
     1441 aaacttatca aattctttat ttgtttctat atttagtttt ttagaaatta aagtatcaag
     1501 gttttggttc ttaaaattaa ttggaatctc atagatattc ttaacatcgt ttgctgaaat
     1561 tatttgttct ggaagtaacc cgcaattatc acatagttta tcaattgatt tcttatcaag
     1621 tattcttttt gatctacaaa gcaaagcatc tggagttatt cctctcttga aaagttcaga
     1681 aacgctgttt tgcgcaggct ttgtcttttg ttcgctactt ggtgcaatct ctggaacata
     1741 tgctagatga ataaaacaaa tattttcttt tccaagtttg tttttaatgc cttttgctgc
     1801 agcaatataa agttcgtttt caagatctcc aacagttcct ccaatctcta aaagcacaag
     1861 ttctgcttta ttgtcaagag cagtcttaag tattctgtat tctatatagt ctgtgacttc
     1921 tggaattaat ttaactgttc ttcctaagaa cattccgctt ctttcaaggt cgataatctc
     1981 tttatagatt ttccccattg ttaggttttg agtgccgtta caagagatgt ccagatacct
     2041 ttcataatgc ccaaagtcca tatctacttc tgtgccatca tcaagcacaa atacttctcc
     2101 gtgctctata gggttcatgg ttccaggatc aaaattaaga tagccgtcgc acttaatagg
     2161 tattttttta agattcttat ctaagagaag agcaacacta gaggcagata ctcctttccc
     2221 aagtccagaa agcacaccgc cagtaacaac tacaagcttt agtttctttt cttttgctaa
     2281 atcataaata ccactgatat ctttatagtc cataataatc acattacaat tagtttagaa
     2341 aattataata taaacagttt atatttgttt ttgtcaatcg ccagatttat atacattttt
     2401 gctctttaaa tatatatttg cggccaattt gttaggttgt tgtgtgaaaa tagttaaata
     2461 ttgcgctagc ctagcgaaac aggtagttaa ccagaagaag aactcctacg aactgggcga
     2521 gtagttgtcg tgcataggca cgaaagatat tgcgcacgaa ttcacagaaa caaagtttct
     2581 gggatgccaa gaaatgcctt tggcatttgt tcagcacgcg cgaaaccgat tcatgcagag
     2641 gtggcggagt ggccaaacgc gccagccttg agagctggag tcgaaagact acgcgagttc
     2701 aaatctcgtc ctctgcgaat ccctttcttt gtaaagaaag cgatttcatt cggaaagaaa
     2761 cacaaaatat actcaaagaa aaaacaacta aaaactaacg atttgattat tatgtcagaa
     2821 gaaaagccaa tcattacttt tgatgagttt tcaaagctcg atataagggt tggaacaatt
     2881 atagaagcag agaaggttcc aaatagtatt aagcttataa gattagttat tgattttgga
     2941 gatttcaaaa ggcaaatact tgcaggcatt gcaaccaagt attctcctga agatttacaa
     3001 ggaaagcaga taccagtagt agttaacctt gctcctcgaa agatggcagg gcaagttagc
     3061 caaggcatga tattagcaat aggcgacgga gaagtagaag cgctacttca accaacagag
     3121 aaagtaaaag aaggcggttc agttcattag gtgaaaaata tggataaagt caaagtaaat
     3181 tattttattg atgtgccttt ggcgctatca tttataattg tagcaattac aagtttaata
     3241 ttattttttt tcttgccaag cggagtaagg caagcaggat atcagatgtt ctggggatta
     3301 acaaaacaca catggagtca gatacacaca atatctggtt ttataatgat aattttatcc
     3361 ttagttcatt taatattaca tttcaaatgg ctaacaacga tgtcaaaaac aatctttaag
     3421 aaaaaataat atttttcttt cccaaacaat aagttttctt tgggactgaa agaactttct
     3481 tttctaaaaa gaaagggcct catgcagagg tggcggagtg gtcaaacgcg ctagcctgga
     3541 aagctagagt ctcacgacta cgcgagttcg aatctcgtcc tctgcgcttt tctatttttt
     3601 tatttcaatt gtttgtgttg gataagcaaa ctcaatattt tctttttcaa actctttttt
     3661 aattgttgag ttaatcttat tttttatagc catatattga tcatattctg cagcttttat
     3721 ccaatatacg actcttatat ttaaagaaaa atccccgaaa gactcaaaac caattacaat
     3781 gctgtcgtgt tctatgtctt tatttttagt taatgtgttt tttataattt cttgggcttt
     3841 ctgtatcttt ttttctgctg ttccgtaaac taaagtaagt aggtagtttt ctcttcttgc
     3901 gcgtcttgca gaaatatttt ctacagagtt cgtagaaagt aatctattag gtataacaac
     3961 aatagtatta tcaaaagttc taacctttgt agacctaatg ccaatctctt caacagttcc
     4021 aaggccttct gaagttttta acgcatcccc taccttaaat ggtttttcta taaatattgt
     4081 aactccagaa attaagtttt ctaatatgtc ttttgcagca aaggcaatcg caataccgcc
     4141 aataccaagg cctgcaagta gtgcagtcac attataaccc agattagata gcacaagcaa
     4201 aaatgcaaaa gtaaacaata taattttaat taatgatttt ataaccggga taatctgatc
     4261 atcatattta gattctgatt tttttgcacg aggttcaaaa taaacttcaa ttaaaccaat
     4321 aaagaactta attaaaaata aagttatagc aaagactaga aaaataaaag ttgccttatt
     4381 tataaaattt tgtaatccgg ttgttgattt taaatattta aatccaatat taaaaaatat
     4441 aatgagtatt attaatttta ttggcattgg tttagacagg attttaacaa taatatcgtc
     4501 aattttattt cttgtatatt ttgttaattt tataaaaatg ttttttacaa taaaaaccat
     4561 cacccaacca actacaaaac ctagggcaat aaaaaataaa aaccacaaat aatttttaat
     4621 tgtatttcca aatataactt gttctaacat gttttccctc aatatatata tatattacta
     4681 caaaagtatt aaaagagtta tttaataatc tttctgtttt ttaaaaaata ctatttataa
     4741 accatatccc ccaataatat atattataat tatataccat aaacgggggt taaaaacatg
     4801 ggaattttaa aagaatttaa agagtttcta caagaataca aagtaattgg cctagctatt
     4861 gcttttgtta taggtgctgc aattacggct cttgttaagt ctttagttga caacatcatc
     4921 atgccaataa tcgcaccatt gataccaggt ggttcatgga gaacagcaat attgactctt
     4981 ggtccattca agattggttg gggcgcattc ttaggcgaat ttctaaactt tgtaatcatt
     5041 gcatttgtaa tctttatgat tgctaagatg attctaaaag aagacaaagt tgcaaagaag
     5101 taaatggaga cgactagtat ggaaagacaa ccagtagatg ataaagactc cagaatgatt
     5161 ggaaatctag tatctatagg ttacgattca gataatcagg tattagaggt cgaatacaag
     5221 ttaggttata tctatcaata ccacaaggtt ccaaaggacg tctacgaaag gctaatatct
     5281 gaaagaaaca aaatagagtt cgttcagcaa agaatagcca aagtttatag aaaccaaaga
     5341 ctaaagtaaa ttactttggt ttttcttttt ttatttttta atctattttt ggcagtattg
     5401 tatttgtttt ttctgttgtt ataaaaacta acaacatttt taaatagttt gtttcaatta
     5461 tatttatgcc acaagatatt atttttaagt tgcaagaaat tgggctatca aaaaacgagt
     5521 ccaccatata tcttgaactt ttgtcaaatg gcgaagcttc tgcaaacgaa ctttctaaaa
     5581 agataaactt tgatagaact ctttgctatc agctattaaa caaacttata gaaaaagggc
     5641 tagttcattg ctatataaaa aaaagcaaaa agatatttaa tattacagac accaaagact
     5701 tgttactgtc aattaggaaa aaagaacaaa tcgcagaaga gctgattgat aaattaaatc
     5761 ttttaaaaac aaaatcaagt cacgaagaac agaatctatt aatttaccaa ggagataagg
     5821 gaatagatgc tgtatttaca gaattactta aggcaaaaga agtatatatc tttggagggg
     5881 ccgcaaaaac ctttaataag aaaaagatga acgaaaaatt aatttcaaaa ttaaacaaat
     5941 taaaaataaa aggaaaagca ataatctctg atcctaaatc ttattctgaa ttgggtttta
     6001 atattaaaga attaatggtt agagaattaa aaggtatgaa taacactgtt acaacaatta
     6061 tttatggaaa caaaataggc atacacattt tagcagacaa tccaattgta atatttattg
     6121 ataataaaac tgttgccaaa tcctacttag agcattttaa aaatctctgg aatcaagcta
     6181 aacccctaac aatataaact cagataaccg atgattacat tacagaatct gataataaag
     6241 attgcagagc ttcaaaaaaa gcacaattac tatattctta tagcagtagt gttaattact
     6301 gtatttatgg ttattggtgc aaccagatta gaaatgcaag gcgacttaag taaagaaatg
     6361 cctcaagaat taccaatctt taaacttaat gaaagaattt ctgataaatt tggagggcaa
     6421 gatattattc ttgttgttct agaagtagaa aacaatagca attctaagtc tgcagtaaat
     6481 gatttaagag atccaagagt tataacctat ttgaaagatt tagaagcaag tctaaagcaa
     6541 acaacaacaa ttgaaaatgc atattctgtt ggaacttttt tacaaggcta taattttgaa
     6601 gacacaacac aagtttcgaa ttttttgtca aggtcgccac agactttgga tttctatagc
     6661 aaggattata agcttacagc aatgtatgtt acaaccgatg tagggtctgg gcaggataaa
     6721 ataattgcta ccactaactt aattactgaa aaaataaatt ctttgtctat gcctccggga
     6781 ataaaagtca cagtaactgg taatgctcca atgagtaata ctcttttaga tattttaaag
     6841 aaagatgcaa tttttacttt gcttctagca gcactaataa tactaataat tttaatgatt
     6901 acagaaaaat ccataccaaa aggattagtt gtatttacac ctttattttt tgggataatt
     6961 tggacaattg gaactatggg ttggcttaat attaaattat ctgttgcaac cgttggaata
     7021 ggtgcaatga ttttaggact tggcgtagaa tatggcgtat tcatgctttc aagatacaaa
     7081 gaagaaagag acaaaggcaa aacacagctt gagacactaa aaattgctgt gccttctgta
     7141 gggatatcaa taatgggttc tgcaacaacc acgattgttg gttttttagc attaacttct
     7201 tcagtcatgc caatgcttca acatctaggc atttctttag ctttaggtat tttctattgt
     7261 tttttagcag cactatttat tgaaccagta attattatta aaatggaaga atgggaatat
     7321 catttagcca acaaactaca cgaaaggttt gctaaaatga aagcttctgc aaaaggtgat
     7381 ttagattgag tctaataaat aagattgtag aagcctattc taaatttgtt tcaagaaatt
     7441 tgtggttaat ggtaattatt gttttaataa tcacaggaat ttctgcgtat ttttcttcaa
     7501 caataaccac taagaatatg gattatagag atatgctccc tgaagacaga gacaccatac
     7561 atgcgctttt tgcaattgaa gaaggccttg gtggaacaga aactgcaatg attgcaatag
     7621 aattggattc gcaatatgct aattcaaatg aaagtagaga tataagagaa ctggagattt
     7681 tagaatatat agataaatta agtaaattgt ctgacgaaat agaagatgta gagtcggtgt
     7741 caagtattac aacaataatg agggcatcaa ataacaggga attacctaaa tcaaaaacag
     7801 aaattaataa ctttatttct aataacccgc aatctaatca atatgtaagt agcgattatc
     7861 agatggctct tataagactt agattaagag atgattttga tgataagcaa atattaaaag
     7921 aaatagaaga caccatttct ttaattccaa aagaagaagg tgtagttgta agtcctgctg
     7981 gtcaggcatt aacaagtgca gcagtaaata atgagatcgg aggagatatc gcaaagactg
     8041 ggcaactttc aatgattgga ataattataa ttttactttt attatttaga tcaataattt
     8101 ttggtttaac tcctctgtct acaattttaa taggagtcgt ttgggcattt ggatatttgg
     8161 gactaacagg aatcggcata agctcaataa cctctggtgc aatttctatg attatgggag
     8221 taggaattga ttttggtata cagatagtca acagatttaa agaagaactt caaaaaacaa
     8281 aagaggaagc aaaagcaatg gagattactt taaacaatgt aattattcca atgggtacaa
     8341 caactatatc tgcacttatc ggttttacag ctatgtctat gggtgaatta acagttatga
     8401 aagatctggg cagcatatta agttatggaa tagccgcttg ttttttagcg tccatttcta
     8461 ttgttccagt attgctaata ttaattttaa gattttataa aaaaataaag gagagaaaaa
     8521 aatgaataag aaatatagtt ttatagttta tgcaagttta ttattgctta taggtttgtt
     8581 aataaaacca atttatgcag cagatgatct agacaccgct tcattaaaag taacgcttct
     8641 taatcaagac cctgaccctg cagagccagg agaatatgta gatattagat ttaaagttga
     8701 aaagtttggt aacaacccat taacaaatat ttcttttgta cttgagccag aatatccttt
     8761 cttgtttgat gattctgatg ttcctgaaag aaatattggc accatcaaat atgcaagcgg
     8821 agatgattgg tactataatc taaaatacaa attaaaagta gactctgaag cactagaagg
     8881 aacttatgat ttaaagctaa aatatgaagt aaacaactca aatgtaaaaa acatagttga
     8941 atttaatatt gcagtaggag atgccacaaa acctgatttt gttgcaggaa acataatctc
     9001 aagcccaaca aaattagttc ctgatactga ctctgcacaa ttaaatattg agataagtaa
     9061 tataggagac gaagatgcac aaaatgtcgt agcaaaactt aatttaccaa cagaattaat
     9121 cccaacatat agctactcag acagatcaaa tcttgcaaca atagcaagcg gcacaagcaa
     9181 agttgcaatt ttctatttag atgtagataa gattgcagaa tcaaaaatat attctgttcc
     9241 gttaattata acctataaag aaaagaacga taacacaaac gagtataaaa caaaaacaat
     9301 aaatatcgaa attccaataa aagaaaagcc agtatttaag gttacacaaa taattacaga
     9361 gccagtaaat gctgctccag gagacaaagt aaaaataata attaatttac aaaatacagg
     9421 taaagatgca gaagcagttt ctttaaagtt atataaggaa tcaagccagc caataacatt
     9481 cgatgaaaaa accgactata ttggaaccct agataaaggg gaaaccggaa ccggaataat
     9541 aagtttagaa atagacaaag atgcagaact taaagactat cttctagatt tagagattag
     9601 agccatagat ggcgaacaag taattgttca aaatgagacc atatctttaa aggtttctaa
     9661 atctaataag cagttgtcta ttatctggat ggtttttgga attgtaattt tagtttctgc
     9721 agctgcaggt ttttatttct ataacaaatc caaagaagat aagaagaaaa agaaataatt
     9781 tctttttctt ttaaacaata tctttatact atttttcttt cataatttta ttatgaatta
     9841 cttatattta gatatcgaaa cttgtccaag agcttttctt caaaagaaag cctcggaaaa
     9901 gaacgaggtg tttttatgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 ccaagagctt ttcttcaaaa gaaagcctcg gaaaagaacg aggtgttttt atgaactact
    10081 tatatttaga tatcgaaact tgtcctatag acaaagaact ttatgattct ttagaagatg
    10141 aagaagcaaa gctaaaatta cttaacccta ttgattcaag agttgttgct ataggcctaa
    10201 aatactcaga cagcaaagat acaattatcc ttcaagacga taacgagaaa aagattcttg
    10261 aagacatgtg gcagaccatt tcaaaattaa gaggaaacgg tacaagctta agaattgttg
    10321 gctttaatat tttaaatttt gatatgcctt ttattatagc aagatgcttt attaataaag
    10381 tttcagttct tccaatttct ggtaacgaaa ttattgattt aagagatctt gtttcttttt
    10441 ttagaacagg aaagactaga ggcgggctaa aagactttgg aaaagccatg ggaatagagc
    10501 tagacgaaaa tgatggcgga gatatagcaa ggctttgctt tcaagaggat aaggaagatg
    10561 gcaaaaagca aatagcaaaa tatcttcgta aagacataga gctaactgaa agtatgcata
    10621 agcaaataat gtctcttggt atagataagc tcagaagata ctaatattaa ctgtttttaa
    10681 atgtttggta atttcttttt attgttgata ttaatttttg aggtgtaaaa taaaatgaaa
    10741 ttaactaaaa tactaatatt tgtttttata ttgcttttta tgcaaaacct ttttgcattt
    10801 aatcctacgt tttatgtagg agatggagtt tctggaacag acccaaattg tcaaaatcca
    10861 aattattctt ctattcaggc agccgctaat agcataaaaa acgacgcaat acaaaatgca
    10921 ataatcattg tttgccaagg cacatataca gaaaacataa atctaacaga ttttgaaaat
    10981 ttatcaatta ttggagagaa tgacgaagta attataaaat ctccagcaac agaagttagt
    11041 ccattacaag tatttttttc aacaaatata ggtcttaaga atttaaaaat aatagagccg
    11101 aatttaaaag gcgttgttct caataatgtt atagatgtaa ttttagaaga cataagcgta
    11161 tcagaaatta aagggatagc acaaggagaa tatagtttag ctgggatata tatttctggt
    11221 tctgataacg tgactcttaa aggagacata aaaattgaag acgtagatgc cagtataaaa
    11281 tatacaaacg gtattgaaat ccggaattct gaaggcattg tttttgatac agatttatta
    11341 gttgtttata atataaaatc cagtaacaat gcaacagatt cattattaac tggaatttta
    11401 ataaataact cagatataga aataaaatca aacgttacaa ttagtgaact taacggaaga
    11461 aaagacgtta ttggtttttt agcagaaaat gactcagtag taactaacga aaacgaaata
    11521 aatatggggg ttactcaatc aaggtctttg tctgtaatta agggatttgc aatagagaat
    11581 tctagaatta atcttaatga caatactcgc acaaattttt tcagtattgg cggatttcaa
    11641 gcagacactt atgcatttta cttagataat gcgcaagttt catggttagg ggaattagaa
    11701 tttataaggg ttattggaga agataagagt tatggactat atataaataa ttcagataat
    11761 tttatagttt caaaaataga tacagacaat actcaaattg aaggagaggt gagattaata
    11821 tatataaaag actctgattc tggtcaaatt aaaaacatta caaacggcaa tgaagaaatt
    11881 atgattacta caaatgattt tagagtttta aatataatta attcttctgt taaggtaaat
    11941 aaaatgagaa taacctctaa tagagatttg ccaaactgta tagttgcaga aaataattca
    12001 aatatcgagt tagaagatat tgatttaaca aattgttctg gaaattatat taaaaataat
    12061 aattcagaga tatcaattaa aaattctcct acaatcaatc ttgcaaaaat acagtatgat
    12121 caaagtcaaa attcaattgt agaagtttat aagccagtaa aattattacc ctataaagat
    12181 ggaagagatt taaaattaga cgaattatac atttatcacg attcaaaata tttctcaaat
    12241 atatttaatc cagaaacaga aatagaatat gatttattat attttacaaa aggctataaa
    12301 aatggagtat tagattctag tttctataat gattattatt ttactggaat aaagtctaga
    12361 gttaatgaaa aaaaagaaaa aatcacaatt aatgattttt cttttcaaga aatatatatt
    12421 gaatttttta ttgtgcctaa cgagttttta gaacaaaccg caaaagcagg ggttaccacc
    12481 aaaaattcag accatttttc tgtcacagat aaaacttgtt caaaaacact tgataaaagt
    12541 tatggttgtt ttgatataaa attgtcttta gctttagaag acacagaaaa tgagagttat
    12601 actcttaaat ttaaattgcc tgtttcttgg ttaacacaaa agaatctaaa gaaagacgaa
    12661 attgtacttt atcatttaga aaataacaaa tatgttgaac aaaaaaccac tcctttaaca
    12721 atagttgata attatcagat attcatttct gaagtagatt ctttctcaga atttcaaatt
    12781 gcagaaaaga aagacgtttc tgatgttgat gtcccaaaga atgatacacc cccagagaca
    12841 acacccccag taacagaacc gccagcagat tcaaatcaaa caaatccaaa ttgtaatatt
    12901 aaatgtacag agaaccaaat tttagattat atcaactgta aatgtataga caaacagaca
    12961 ggagtctgtg atttagtttg cgattcaaac gaatatttag atacagagtt atgtcaatgt
    13021 tttgatttag caaatgccgg ttgtagaggt gcttgtaatg ttggagaaac aatgaattta
    13081 acaacctgta cttgcgttcc tgttgttctt gacggcatgc caatttctca agcaaacaat
    13141 ttctggagtt ttgttcttat agttttagta atagtaattg caattatagt tttcttaata
    13201 attaggaaaa acagaaaaaa taagttaaaa aagcaatcaa agaaaaagaa ataattttct
    13261 ttctttttct taatccaaat ctttaaaaac cctaatatat tatatttctt tagtttctta
    13321 tattattaga tattgataaa tatggctata gaaaaaacca aaagcgatgg gacagatcct
    13381 tttgaaaaga tagagaaagg taaaaacgac aaaagtatct atgagtccgt tccagaagaa
    13441 gaaaaagcag atgaaaagaa aaccaaaacc actgcgcgcg agcccgagcg aaaccggctc
    13501 aaagagatta caccaaaact tgaattttta acagatccag aagatgaaac aatagtctat
    13561 atcggaagaa aaaaatcagt tctaaaagaa catggctcaa agtcttgtgc gtttttaggt
    13621 aaagtctatg aagatgattg cagagacaag aaagtttatt tagatggtct taacccacac
    13681 gtagtattta ttgatggttc aagaggttca ggaaaaagct atggccttgg agttattgct
    13741 gaagagctaa ctacaaacaa cccatatatc ggccagatag ttgttgatcc agttggagtc
    13801 ttttggtcaa tgaaataccc aaatcaagaa aaaaaagaac ttgaggaatt aaaaaaatgg
    13861 gatttacaag caaaaggcct aaataatatt aaagtctttg tgcctgaagg agtggttggc
    13921 aaagtctcta aagaaacata tgacgaaacc ttttctatgt atccttcaat gcttcttgca
    13981 gacgactggt gcctaacttt tggaattgat agatttaatc caacagggtt attattagaa
    14041 aaaactttat cttttgttaa gcacggttat aaatcaggag acccaagcga gcaagaaaaa
    14101 acaacagaac agattcctgc aaaaggcaat ctctataatc ttgacgatat agttttttgt
    14161 ttacaaaatg cttatgaatt taagtcttct tcttctggtt acaagcaaga ctctattaga
    14221 gcattagttt ctagatttga agccgcaaag tcttggggca tctttagtga tcatggaact
    14281 cccctttcaa aactttcaaa agaaaagcaa ctaacaattc ttgatactag ttttttagat
    14341 gataatatta ctgctcttgt tattggtata ctagcacgaa gaatactttc tgcaagaaag
    14401 cttgtgacta gacaagacgc aattaaaaag tttgattcaa attcagaaag cccagacgag
    14461 ctacttgaaa tttcaattcc cccaacctgg gtatatattg atgaagccca cactcttatt
    14521 ccttcaggaa acaccataac tcctgcaact aatgccataa ttgaatatgt taagcaagga
    14581 agaagacctg gctgttcaat ggttctagct actcaacaac cttcagcaat aaattctaaa
    14641 gtattatctc aagttgatat tttcttaagc cacaaattag tatttaacga cgatattcat
    14701 gctgtccaga aaagaatgcc agcaattatt cctgaagcct acaaggaccc aaattttatt
    14761 aaaactcttc cagtcggtgt ggctttaaca ggagacagag gcgaagaaac caaaagagct
    14821 tttattctta agattaaacc aagattaagt cagcatgaag gcagagacgc agaaaccatt
    14881 agagaggttc caactctttc caagaaggaa ataatcaatt tctgctcaga catgatctac
    14941 aaaaaattat tgtcttcaaa aactcttaag actagtgatg ctaaagacat tcttgatgtt
    15001 gttaattcta aatataatag cgatgttgtt tttgatgaga ttttagtatc tctaagcaag
    15061 atgggaattt ctgaaaaaga caacattttg tattttgaaa ataaaaacac taaacaaaaa
    15121 acagaaatcc ctaaagatga aattcaagat atcaatcgcg caattgaaaa atcaaatgag
    15181 ctttcagaaa tatcagaaga tatgccatta actccagaag aaattgtgcc agacagcctg
    15241 ccagtaatcc cttcaagaat ctcaagagac atagcaataa atattgcaaa cagcacaaat
    15301 aagaaaaagt ttttattttt aagagaagaa ggaattattt ctcaagttac tatgatttat
    15361 agaaaagttt acaagattaa ttttaatatc tttgatgaag acgaaaacta taaaacttca
    15421 acttgcttta ttgattctgt gtctggagag tttatacatt ttgatggcag aaactttaaa
    15481 gcttcaaacg gctttaagtt agttgaagag ctaagcgttg acgatattgt tcttttagat
    15541 agtatctttg cttggaaaac ccctgaagaa ttatctgaga ttttaggatt tgatgcaaga
    15601 gaaataaaac aagacttgat ttctttagtt gatagaaacc ttgctgacgt tgatattatt
    15661 aataaaaaag aaagctttag aataaagaga agaatagatt tgccattcca cccaggatat
    15721 gatattctta cttcaataaa ccatttacca ttaaagaaac aattaatttc taaatctgat
    15781 attcaagaag aaatatccga agaaatcatt gttagcagat tagaaaaatt atggccagac
    15841 atcgagataa cggaaatctc tgagatcttc tggcctcttt gggaaattat ctcaaaagac
    15901 aagagagcaa atcaaagagc aattattgtt gatgcagtta ttggtcaaat cattgatgca
    15961 taaaagtgaa ataatcatgt tagatctact taagaaaaaa gaagaaaaat ctttaagcat
    16021 tgattctttt attttagata gctgtaaagc aacgccaggt tttgagttct ttccaaaaaa
    16081 gccaataaag attgatttag aaaaattatt aatgaaacta agaactaaaa attactatat
    16141 agaaaaagat aattctccgt ttttaataat tttaaaaaca aaaaaaggca cagtaactat
    16201 cttttcatct ctaaagataa ttatcagaga caccactcag gaaaaagttg ctaaagtcat
    16261 tttaacagat cttttaaaaa ccataaacga atttttatat aagcaagggt gactgagtct
    16321 ggttgaaggt gctagtctcg aaaactagat gcgtgtaaca gcgcacatgg gttcaaatcc
    16381 catcccttgc ggagcctttt tcaaaagccg cgggaaaaac gtggttttac tagacttgcg
    16441 tgcaatggtt aattactttg tgtttaaaga aaaaaaaatt ctaaggcaaa aacctaatta
    16501 aaagaaagta gttatataaa gctattgtcc taattattta tataaattta aaggtgatta
    16561 aatgaataa
//
LOCUS       DAOOON010000017        13634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_5532_length_13634_cov_17.088151, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000017 DAOOON010000000
VERSION     DAOOON010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5532_length_13634_cov_17.088151"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLK55_03010"
     CDS             complement(<1..385)
                     /locus_tag="PLK55_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HOZ35926.1"
                     /translation="MEHILEYFVGGGYNLVNTTVYAAIVFTGYYLFYKAIKKYASFVK
                     IDKHFLWGVLVFVFFGSLLRILEQNYTGVWLINSSNSPLEIGFYFHTPGWLILLSFVF
                     LVSFLLSIILFKEKYYRLLIPIGCCL"
     gene            451..1476
                     /locus_tag="PLK55_03015"
     CDS             451..1476
                     /locus_tag="PLK55_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HOZ35927.1"
                     /translation="MNTIKIAKKQAQEVIAKLREENNLDNSYKIKRLEEHIIIPVLKI
                     TSELKEFEVDEELEKQKNIKKSFKDLLLEDGIAPEMVSQINSAYDIVGDIIIIGIDKE
                     LKAYYPAIGKALLKLHKNARTVLNKIGEHKGIYRTQDMELVSGVDTRETIIKENGCRI
                     KIDVEKVFCSTRLSEERRRIVSLVENEETIGVFFAGAGPFAIAIAKNKEVKEIVAIEL
                     NDLAVKYLIENIKLNHVEGLVIPVLGDVKIEAEKYPDYFDRTIMPLPKSAELFLDSAI
                     FSAKDGGIIHIYKFVPKDDPYTETEKEIEGIAKKNKVILEIMSKRIIRSYSPSIVQIV
                     MDLKLKK"
     gene            complement(1479..3656)
                     /locus_tag="PLK55_03020"
     CDS             complement(1479..3656)
                     /locus_tag="PLK55_03020"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35928.1"
                     /translation="MKKKIIFIFLFLIMLSANIFAYIPKAQMKIFAVNNSNAGMDANL
                     IIEIEPGTGKIYSNVNSQVGSLTQESERNAVNAAERVVKDTKGKYDYLFEIQSAASSI
                     DGPSAGAAMSLLLVSMLSDKDLSGKVSITGTITEDGYVGEVGGIGAKAKKAAETGIKL
                     FMIPIGTRKQAITTDSGNSQIVDLPEYAFDKWGMKIIEVETIEDIQKYVSIDIDDIDI
                     NLTKEATEQEYTPTPIEYSKALEPMRSLVDKYLVDANKVLEKTESNINISKIKDSSTV
                     QSLLSLVDYSKESISNAHKYSAGNYLYTAANEAFLAKIYLIAIDEVVSNPSILTADST
                     IYNLRLKEIEDRIELTENRSKSCSLDKIEWCISARQRIVWAKNKIKDIKENSKDGAPL
                     DRIMDYSYALGWIEIANDFLDIGVSTDKEDIKFVESSEFKELAQQYIVNLENEIVLLD
                     TTISQDDDIQRRLKAAKTDYEMGWYVTSIYDAASAKAVINSRKETNTEMFDENLFISK
                     YNSMTFLSSEENLKTEKNIWSKLYYDHATHYYKSYNYYKNKNTTKAQSNIKIAMSIIG
                     LSVELYDVENKILDYYNKGEMVIEVIDSDKKETTENNTITNVNSSQEKVYVYKNNNDT
                     TIFWIIAIGLFLMIIAIVIDNEIRRNSRHQKIQSSLLELEKKLSEGRISKFTYEDMKK
                     SYLKELKEIEQRNENPRLKRYQQPQEVEKPKVKSRKKITKKKR"
     gene            complement(3681..4160)
                     /locus_tag="PLK55_03025"
     CDS             complement(3681..4160)
                     /locus_tag="PLK55_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOZ35929.1"
                     /translation="MTKEKQSLEDIKCAIEAILFMSPRSIPIKKIEKSFPEIDKEVIR
                     IILSDLKEEYEKKNTAIKIIIDGDSAEMLLKPDYAKFSVFATGKQLTKSELKTLALIA
                     LNSPVDQCKITKRRPNDHLPTLKELGLIQVYKKGRRNVLETTKKFDILYNKKAKPVY"
     gene            complement(4171..4842)
                     /locus_tag="PLK55_03030"
     CDS             complement(4171..4842)
                     /locus_tag="PLK55_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOZ35930.1"
                     /translation="MEQLINIIDQPNWKIILYDLVKSNEIDIWNVDLINLTDLYLEKI
                     RNLKEENLIIPANALLAAAILLKLKAYSLKLTAADNNEEELKLLTDDNYILSNSVDLN
                     TPMRLKEGQVSLDELIDVIDIMMNAPTKKNVDRKLKEKKEIEFVLPKRTIDFSERSDS
                     LLAEIKDNADKYNMVLFSSFTKSEKDPYLIVENYFIPLLFLVQDKKIDAWQEDFFSEI
                     FIKIL"
     gene            complement(4860..5285)
                     /locus_tag="PLK55_03035"
     CDS             complement(4860..5285)
                     /locus_tag="PLK55_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35931.1"
                     /translation="MFDKKGQVSIEILIILAMLVVGGVIFGVYYVNHLNSSISNQNDN
                     DVDDATDTFIDSINRFSVEIMSPISDGTYSAGTQIQFTAGFTNNNGSVDCNWSAGSTQ
                     ILTNTCSDTNELSPGNYTIVVTASDGYQIATDSVRIIVK"
     gene            5395..6105
                     /locus_tag="PLK55_03040"
     CDS             5395..6105
                     /locus_tag="PLK55_03040"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOZ35932.1"
                     /translation="MKRVPTGVTGFDELIQGGMPEGATILVSGGCGSGKTIFATTYIY
                     EGARRFGEPGLFVTVEDNLKNIVWNMENFNWDIKSLEQKNLMKIYRMHIDPRKDVESQ
                     VDKEMDIISSMVKDIGATRLTVDSTTAFGGWINDAGRLRNLLFRFGDYLKDLNCTTMM
                     TTETNGGARDFSAYGVEEFVVDGVFALYFIPPHRTIFVRKLRGTKHSPAVHPCEITTS
                     GLVVNPRDEVLWSAIKQY"
     gene            complement(6186..7928)
                     /locus_tag="PLK55_03045"
     CDS             complement(6186..7928)
                     /locus_tag="PLK55_03045"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOZ35933.1"
                     /translation="MNFDVISEYFEKISKTNSRLEMTEILKKLFLETSKEEIQDLIYF
                     CQGTLGPKHKTKEINIGQATLISLVSEYLSIPQEEIKKEFYTLGDIGLVIEKHHSKRK
                     QSTLFYKPLLFREVFETFQKIAETTGTGSTEKKQQLFKFVLFNSSPASAKYLVRFPIS
                     FRLGFGDSTIIDALASLSTIEAEEQKIAKEAITEKYQLVSDLGLIGKIIKEKGIKGID
                     SLKIMPFIPIKSQLCERAESLKDIHERLKVIAVDTKIDGFRQQIHKKGNEIKIFSRQE
                     EDITKMFPEIVEAVKRIQHDFIIDCESIAYDPIKKKYHTFQITITRKRKYDIGEKSKE
                     LPLHLKVFDCLYLDGKDIYYLPFRERRKFVEKYFNHAPFVAPTKILITSKLEDLENFF
                     NTAIKDGFEGIIAKDLEASYKAGSRGYSWIKFKKSYKGSESLDTIDAVIVGAFFGQGK
                     RTDKGIGALLVALYDKEEDRYFTLAKIGTGLTDATLLDLAEKLESIKLETRPKNLVTN
                     ISPDFYVYPEIVVEINYDEITKSPIHTASFDPETKQGLALRFPRLVKVRTDKSSHDTT
                     SQRELEKLFRMQEK"
     gene            complement(7950..9413)
                     /gene="proS"
                     /locus_tag="PLK55_03050"
     CDS             complement(7950..9413)
                     /gene="proS"
                     /locus_tag="PLK55_03050"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOZ35934.1"
                     /translation="MASNEELKIKDFNEWFNDINQRAELADLRYNIKGVVVYRPWSTF
                     TLNKIFRLFEDIFDETAHQQVMMPVLIPEENFFKEAEHVEGFTPEVFWVTEHGDEKLE
                     QRYALRPTSETAFYYMFAYWLESYRDLPIKTYQRANIFRYDTKQTKPFFRGREFYWFE
                     THNIFETYDDSYAQVLQDMQITEDIIHLELGIPFIFFKRPQWDKFAGAEDTFAADTMM
                     PNGKACQLATTHLLGQKFTKTFNVKFRDKDEETKIPYGTCYGPGITRMYGGLIGVHGD
                     KNGLRLPFELAPIQIIIVPLYFSGQDIEQLEKICLDFASDLDKLGYRVKIDDNKEKKP
                     KERFSFYEMKGVPLRIEIGPKDIEESQVVLFRRDLNTKEKVLFADLFDSLKKISMEFT
                     DNLRSQADDDFEESLAIATTKEELVSEINKGKLVRIPFCSIDPDGKKCFDELKAATDG
                     GEVRGIRMDITEVPEHDEQCVICGNKAKHYVYVARSY"
     gene            complement(9475..10110)
                     /locus_tag="PLK55_03055"
     CDS             complement(9475..10110)
                     /locus_tag="PLK55_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35935.1"
                     /translation="MVKTIAKIRTSKQLEIWSLVKKKQKSTSQREIWGKTINKPKKAK
                     VPDALLKNFKKIKTAKNTADRKISELSNDLAAKLRHVDPRYPNDYLNRQIVKLKTKLR
                     IWGLSKRMLSRLDSPTIDLNKLAEPLNSNIQRLFKFEKTNSFQKEILSSLNSLAEKCS
                     SNSTSTLEKREAYLEIIQNIRNLHVSLYNTHVSRGKLIKEFNELMDNLFGK"
     gene            complement(10123..10196)
                     /locus_tag="PLK55_03060"
     tRNA            complement(10123..10196)
                     /locus_tag="PLK55_03060"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10159..10161),aa:Pro,seq:tgg)
     gene            complement(10248..10928)
                     /locus_tag="PLK55_03065"
     CDS             complement(10248..10928)
                     /locus_tag="PLK55_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35936.1"
                     /translation="MIMVNEELRKWAENKSAEGYSPDQIKEALIKEGYTTEEINEAMN
                     IENNNSIIAPVSVTMPKRNKILPIIIIGIIVLILISAGLYFFVFNNERSVSENVNSAT
                     QKDINLVDENGDNFGQMHIDVNVDMEVVTEEGYEDFKQKFASCQSASVESKLFETITY
                     YYEIIGPKEGLCEVKSKFTANPNPEWVNKEMICLYDNSLDFETAVQDMSRCSGELYDL
                     MTNPGSYQ"
     gene            complement(10971..>11624)
                     /locus_tag="PLK55_03070"
     CDS             complement(10971..>11624)
                     /locus_tag="PLK55_03070"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOZ35937.1"
                     /translation="SYMHNKFCVFDNNISLIGSTNFTENSFSQSYNNILVFENTELSK
                     TLKEKIDSFYLGNFSKSSSVLGKTFNENFSIYFCPNENCKTQVLTQISNAEKSIVCMM
                     YSFTLDDFGDALVEERKTGTNIKVILETQQTSQYSEYQKLKAEGIEVIEDNQPYLMHN
                     KFCVFDGKVLTTGSMNFSNNGTNNNDETLLIIKDQNISKQYLDNFQMHWQAWNNQVN"
     assembly_gap    11625..11724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11725..11922)
                     /locus_tag="PLK55_03075"
     CDS             complement(<11725..11922)
                     /locus_tag="PLK55_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35938.1"
                     /translation="MKNKNPYLFFFLLLIILLILIFLILLYFENKPADNGLPNQIQNP
                     LNLGHGGGVSPSLVLFCPEDSC"
     gene            complement(11919..>13634)
                     /locus_tag="PLK55_03080"
     CDS             complement(11919..>13634)
                     /locus_tag="PLK55_03080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOZ35939.1"
                     /translation="HKVIEQASLIPENDNTVLFTTAGMHPLVPFLLGQPHPLGKRLVD
                     YQLCIRTTDIDQVGDATHFSMFEMLGNWSLGDYFKKESISYSYEFLTSKDWLGLDKNR
                     LAVTVFSGNNDVPIDKETIIHWLEQGIPKERIVAIEDNWWGPAGGVSGPCGPDTEIFY
                     WKDNSKPAPEVFDPNDETWVEIWNNVFMEYNKTEEGKFEELAQKNIDTGLGTERVAMV
                     LNDLDDAFKVGKIKEIYDFVLDLAKDKKNTKAIKIITDHLRAATFILADSRGAIPSNT
                     DQGYILRRFIRRAIRYGRLIGIENNFCSKVSKKIMELYDQEYFHLRLKFEFVLQELEK
                     EENKFTTTLENGLKEFNRMTETQKELSGKDAFLLYQSFGFPIEMIEELGKEKNISVDV
                     SGFIEEEKNHKDISRAGAEQKFKGGLAEYTETTTRYHTTTHILNQSLRNVLDKDIKQK
                     GSNITNERLRFDFNFERKLTNEEIKAIEDDVNKIIAKELEVIKESKLLSEALESGAQA
                     EFGTKYPETVFVYSIGDHSKEICMGPHVDNTKELGKFKIIKEEGIAAGIRRIKAVLEP
                     NTDKT"
BASE COUNT         4148 a         2257 c         1982 g         5147 t
ORIGIN      
        1 ctagacaaca gccaattgga attaataatc tgtaatattt ttctttaaaa agaattattg
       61 aaagcaaaaa tgaaaccaga aaaacaaacg agagaagaat aagccaacca ggcgtgtgaa
      121 aataaaaacc aatttctaaa ggggaattgg aagagtttat taaccacacg cctgtatagt
      181 tctgctcaag tattctaaga agagaaccaa agaatacaaa gacaagcact ccccaaagaa
      241 agtgcttgtc aatctttaca aatgaagcat attttttgat tgccttgtaa aatagataat
      301 aacccgtaaa cactattgcc gcatagactg tggtatttac tagattatat cctcccccta
      361 caaaatactc taaaatatgc tccatttaaa ttattttaga ttttaaatta tatataaaac
      421 ccagttattg atttttttgt gaaaatgaaa atgaacacca taaaaattgc aaaaaaacaa
      481 gcacaagaag taattgctaa actaagagaa gaaaataatc ttgataatag ctataagatt
      541 aaaagacttg aagaacatat aattatacct gtacttaaga ttacgtcgga acttaaggaa
      601 tttgaagttg acgaagagct agagaaacaa aagaacatta aaaaaagctt taaagatcta
      661 cttcttgaag atggcatcgc tcctgaaatg gtatcccaaa taaacagcgc ttatgatatt
      721 gttggagaca tcattattat tggaattgac aaggaattaa aagcatatta tcctgcaatt
      781 ggaaaagcat tactaaaatt acataaaaat gcaagaactg ttcttaataa gattggagaa
      841 cataaaggca tctacagaac acaagatatg gaattggtgt ctggagtaga cacaagagag
      901 acaataatta aagaaaatgg ctgtagaatt aagattgatg ttgaaaaagt tttttgctct
      961 acaaggcttt cagaagaaag aagaagaatt gtttctttgg ttgagaatga agaaactatc
     1021 ggagttttct ttgctggtgc cgggcctttt gcgattgcga ttgcaaaaaa caaagaagtt
     1081 aaagaaattg tggctattga acttaatgat ttagcagtaa agtatttgat agagaatatt
     1141 aaactaaacc acgttgaagg cttagtaata cctgtgcttg gagacgtaaa gattgaggcg
     1201 gagaaatatc ctgattattt tgatagaacg attatgccct taccaaaatc tgcagaactg
     1261 tttttggatt ctgcaatctt ttctgcaaaa gacgggggca taattcacat atataagttt
     1321 gtgcctaaag acgaccccta cacagagact gaaaaagaga ttgaaggaat tgcaaagaaa
     1381 aataaggtta tcctagaaat aatgagtaaa agaattataa gaagttactc cccttcaatt
     1441 gttcagattg ttatggattt aaaattaaag aaataacttt atcttttctt ctttgtaatt
     1501 ttctttcgag attttacttt tggtttttca acttcctgtg gctgttgata tcttttaagt
     1561 cttggatttt catttctttg ctctatttct tttagctctt ttaaatagct ttttttcata
     1621 tcctcataag tgaatttact tatgcgtcct tcacttaact tcttttctaa ttctaaaaga
     1681 gaagattgta ttttctgatg tctgctattc ctacgaattt cgttatctat gacaattgct
     1741 attatcatta aaaacaaacc aatagcaatt atccaaaaaa tggttgtgtc gttgttattc
     1801 ttataaacat aaaccttttc ttgggaagaa tttacatttg tgatggtatt attttctgtt
     1861 gtttcttttt tatctgaatc tataacctct attaccattt ctcctttatt ataataatct
     1921 aaaatcttgt tttcaacatc atataattca acagaaagac caataattga cattgcgatt
     1981 ttaatgttac tttgagcttt tgtagtgttt ttattcttat aataattata gcttttatag
     2041 tagtgtgttg catgatcata atacaatttt gaccaaatat ttttctctgt ttttaaattt
     2101 tcttcggaac ttaaaaaagt cattgagtta tattttgaga taaataaatt ttcatcaaac
     2161 atttctgtat ttgtttcttt tctagaattg attactgctt tagcagatgc tgcatcataa
     2221 attgaagtaa cgtaccagcc catttcataa tcggtttttg cggcctttaa tcttctttga
     2281 atatcatcat cttgtgaaat ggttgtatct aacaaaacaa tttcgttttc taaattcacg
     2341 atgtattgct gtgctaattc tttaaattct gaactctcca caaactttat atcctcttta
     2401 tctgtcgaaa ccccaatatc taagaaatca tttgctattt ctatccagcc aagagcataa
     2461 ctataatcca taattctatc aagtggtgct ccatctttac tattttcctt aatatctttg
     2521 attttatttt ttgcccaaac aattctttgc cttgcactta tacaccattc aattttgtct
     2581 aaagaacaag attttgatcg attttctgtt aactctattc tgtcttctat ttctttaagt
     2641 cttaaattat atattgtaga atcggcggtt agaatagaag gattacttac cacttcatca
     2701 attgcaatta gataaatctt tgcaaggaat gcttcgtttg ctgcagtgta cagataattc
     2761 cctgcggaat atttatgtgc attagatatc gactccttgg aataatcaac taaagacaat
     2821 aggctttgaa cagttgaact gtcttttatt ttactaatat taatgttgct ttctgttttt
     2881 tctaaaactt tgtttgcatc aactaaatat ttatctacta atgatctcat tggctctagt
     2941 gcttttgaat attcaattgg tgttggtgta tattcttgtt ctgttgcttc ttttgttaag
     3001 ttaatatcaa tatcatcaat atctatggaa acatattttt gaatatcctc tatggtttca
     3061 acctctatta ttttcatgcc ccacttatca aatgcatatt ctggaagatc aactatttga
     3121 gagtttccag aatctgtagt tattgcttgc tttcttgtac ctattggtat cataaataat
     3181 ttaattccgg tttctgcagc tttctttgct tttgctccaa tccccccaac ttctccaaca
     3241 taaccatctt ctgttatggt tccagtaata cttacctttc ctgaaagatc tttatcgcta
     3301 agcatagaaa ctaataataa agacattgca gcacctgctg atggtccatc tatactgctt
     3361 gctgcagatt gtatttcaaa taaataatca tattttcctt ttgtgtcttt tactaccctc
     3421 tctgcagcat ttactgcatt tctttcagat tcttgagtta atgaaccaac ttgagaatta
     3481 acatttgaat aaattttacc agttcctggt tctatttcaa taattagatt agcatccatg
     3541 cctgcattag aattatttac tgcaaaaatt ttcatttgtg cttttgggat atatgcaaag
     3601 atgtttgcag atagcattat taaaaacaga aatataaata ttattttttt cttcatttat
     3661 tctcactttt ttattacttt ctaataaact ggctttgctt tcttattata taaaatatca
     3721 aactttttag tggtttctaa aacatttctt cttcctttct tgtaaacttg tatcaatcct
     3781 aattctttaa gggtagggag atgatcatta ggtcttcttt tagtaatctt acattgatca
     3841 acaggagaat ttaaggcaat taatgctaat gtttttaatt ctgattttgt tagttgttta
     3901 cctgttgcaa atacactaaa tttagcataa tctggcttaa gtagcatttc tgcagaatct
     3961 ccatcaataa taattttaat tgctgtattc tttttttcgt attcttcttt taaatctgaa
     4021 agtattattc ttatgacctc tttatctatt tctggaaagc tcttttcaat ttttttaatt
     4081 gggatgcttc tcgggctcat aaatagtatt gcttcaattg cgcatttaat atcttcaagg
     4141 ctctgttttt cttttgtcat attttctcag ttataaaatt ttaataaata tttctgaaaa
     4201 aaagtcttct tgccaagcat ctatcttctt gtcttgtaca aggaacagga gaggtataaa
     4261 gtaattttca actattaagt atgggtcttt ttcagatttt gtaaaactag agaaaagaac
     4321 catattatat ttgtctgcgt tatcctttat ttcagctaat agactatcgc ttctttcaga
     4381 aaaatcaatt gttcttttag gaagaacaaa ctctatttct tttttttctt ttaattttct
     4441 atcaacattt ttctttgttg gggcattcat catgatatca ataacatcaa ttaactcatc
     4501 aagactaact tgcccttcct ttaatctcat tggtgtattt aaatcaacag aattacttaa
     4561 tatatagttg tcatcagtta ataattttaa ttcttcttcg ttattatctg cggctgttaa
     4621 ttttagagaa tatgctttaa gtttaagaag aattgctgca gcaagcaaag cattagccgg
     4681 aataattagg ttttcttctt ttaaatttct aatcttttct aaatataaat ctgttaaatt
     4741 aatcaaatcg acattccata tatcaatttc attagactta acaagatcat agagaatgat
     4801 tttccagttt ggttgatcta taatattaat taattgttcc atgtttctct tatattgctt
     4861 tatttaacga ttattctcac agaatctgtt gcaatctgat atccatcact tgcagttact
     4921 actattgtat aatttccggg agataattcg tttgtgtcag aacaggtatt tgttaaaatc
     4981 tgtgttgatc cggcactcca attacaatct actgaaccgt tattgtttgt aaaaccggca
     5041 gtaaactgta tttgggttcc agcactatat gttccatcag atattggaga cataatttct
     5101 actgaaaatc tattaattga atcaataaat gtgtctgtgg catcatctac atcattatcg
     5161 ttttgatttg aaattgaaga attaagatga ttaacatagt atactccaaa gataactcca
     5221 ccaactacta acattgctaa aattattaaa atttctatag atacttgccc ttttttatca
     5281 aacatattta ccccttcaaa tttaattgac tactatagaa taatattaac aataggtata
     5341 taaatatatt ataccaaata taatatacaa aaataattaa aaaaggtgta aaaaatgaaa
     5401 agagtaccaa caggagtaac aggatttgat gaattaatac agggcggaat gccagaaggc
     5461 gcaacaattt tagtttctgg tggttgtggt tctggaaaaa ctatttttgc tacaacttat
     5521 atctatgaag gagcaagaag atttggagaa cctggattat ttgttactgt tgaagacaat
     5581 cttaaaaata tagtttggaa catggaaaac tttaattggg atattaaatc tttagaacag
     5641 aaaaatctta tgaagattta cagaatgcat atcgatccaa gaaaagacgt tgaatcacaa
     5701 gtagacaaag aaatggatat aatttcttct atggttaaag acataggggc aactagactt
     5761 acagttgact ccactactgc ttttggtggt tggataaacg atgccggtag acttagaaat
     5821 cttttgttta gatttggaga ttatttaaaa gacttaaact gtacaacaat gatgactaca
     5881 gaaacaaacg gtggggcaag agacttctct gcttatggtg ttgaagaatt cgtagttgat
     5941 ggtgtttttg cgttatactt tattccccca cacagaacta tatttgttag aaagcttaga
     6001 ggaacaaagc actctccagc agtacatcct tgtgagataa caacatcagg cttagttgta
     6061 aatccaagag atgaagtttt atggagtgct attaagcaat actagaaccc ctttaaaaaa
     6121 gtggttttca gtcccaaaac gactggttgc ttatttcttt ttcttatttt ttaaaaacag
     6181 aaaaactatt tttcttgcat tctaaatagc ttttctagtt ctctctgact ggttgtatca
     6241 tgagagcttt tatctgttct aactttaaca agccttggaa agcgaagagc taggccttgc
     6301 tttgtctcag gatcaaaact tgcagtgtgt attgggcttt ttgttatttc atcatagttt
     6361 atctcaacca caatctctgg ataaacataa aaatccggtg aaatatttgt tactaaattt
     6421 tttggtcttg tttcaagctt tatggactct agcttttctg ctaagtctag aagtgttgca
     6481 tcagttaggc cagttccaat ctttgctaac gtgaaatatc tgtcttcttc tttatcataa
     6541 agcgcgacaa gaagtgcacc aatgcctttg tctgttcttt tgccttgacc aaagaaggca
     6601 cctacaatta ctgcatcgat ggtatcaagg ctttctgagc ctttgtaaga cttcttaaac
     6661 tttatccaag aatagccacg agagcctgcc ttatagcttg cttctaagtc ttttgcaata
     6721 attccttcaa aaccatcttt tatagcagta ttaaaaaaat tttctaagtc ttcaagcttg
     6781 cttgtaataa gaatttttgt aggtgcaaca aatggcgcat gattaaaata cttctcaaca
     6841 aattttcttc tttctctaaa tggaagataa tagatgtctt taccatcaag atataaacaa
     6901 tcaaaaacct ttagatgtaa aggaagctct tttgatttct cgcctatgtc atatttcctt
     6961 tttcgcgtta ttgttatttg aaaggtgtga tatttttttt ttatggggtc atatgcaatt
     7021 gactcgcaat caattataaa atcatgctgt attcttttaa ctgcttctac aatttcagga
     7081 aacatctttg ttatgtcttc ttcttgtctt gaaaagatct ttatttcgtt tcctttctta
     7141 tgtatctgtt gcctaaagcc atctatcttt gtatcaactg ctataacttt tagtctctcg
     7201 tgaatatctt taagactttc ggctctttca caaagctgtg acttgattgg aataaatggc
     7261 ataatcttta aagaatcaat tccttttatc cctttttctt taattatctt tccaatcaaa
     7321 ccaagatcag aaactagttg atatttttca gtaatagctt cttttgcaat cttttgctct
     7381 tctgcttcta ttgttgaaag gctagctaat gcatcaatta ttgtggaatc tccaaagcca
     7441 agcctaaaag atattggaaa tcttactaga tattttgctg aagcaggact tgagttaaaa
     7501 agaacaaact taaacagctg ttgtttcttt tctgtggaac cagtacctgt tgtttctgca
     7561 atcttttgaa aggtctcaaa gacttctcta aataataagg gcttataaaa aagagttgat
     7621 tgttttcttt tagaatggtg cttttcaata acaagaccaa tgtctcctaa agtataaaat
     7681 tcttttttta tttcttcttg aggaattgaa agatattctg aaactagaga gattaaagtt
     7741 gcttggccaa tatttatttc ttttgtttta tgttttggac caagagtgcc ttgacaaaaa
     7801 taaattaaat cttgaatttc ttcttttgaa gtttctaaaa aaagcttttt taaaatttca
     7861 gtcatctcta gacgactatt agttttactg attttttcaa agtattcaga tataacatca
     7921 aaattcaatt aagaatcact tcttttattt tagtagcttc tagcaacata gacatagtgc
     7981 tttgctttgt tgccacagat aacgcactgc tcatcatgct caggaacttc tgtaatatcc
     8041 attcttattc ctctgacttc tccaccatct gttgctgctt ttaactcatc aaaacatttt
     8101 ttcccatctg ggtctataga acagaaagga attcttacaa gctttccttt gtttatttca
     8161 gaaactaatt cttcttttgt tgttgcgatt gctaatgatt cttcaaaatc atcatctgct
     8221 tgacttctta aattgtctgt aaactccata gatatttttt ttaggctatc gaaaagatca
     8281 gcgaataata ctttctcttt agtatttaga tctcttctaa ataatacaac ttgagactct
     8341 tcaatatctt tagggccaat ctctattctt agtggtacgc ctttcatctc ataaaatgaa
     8401 aatctttcct ttggtttttt ttctttattg tcatcaatct ttactctata acctaatttg
     8461 tcaagatcgc ttgcaaaatc taaacatatt ttttctaatt gctcaatatc ttgaccagag
     8521 aaatataggg gaactataat tatctgtatt ggcgccaatt caaaaggcaa tcttaaacca
     8581 ttcttgtctc catgtactcc aattaaaccg ccatacattc ttgttatgcc aggaccataa
     8641 caagtaccat aaggaatctt agtttcttcg tctttatctc taaacttaac attaaatgtt
     8701 tttgtaaatt tctggcctag aaggtgtgtg gttgctagtt gacaggcttt gccgtttggc
     8761 atcatggtgt ctgctgcaaa ggtgtcttct gctcctgcaa atttatccca ttgtgggcgt
     8821 ttaaagaaaa taaatggtat gcctagttct aaatgtatta tgtcttcagt tatctgcatg
     8881 tcttgaagaa cctgtgcata tgagtcgtca taggtttcaa agatattgtg cgtttcaaac
     8941 caatagaatt ctctgcctct aaagaaaggt tttgtttgtt ttgtgtcgta tctaaagatg
     9001 tttgcacgct gataggtttt tattggaaga tctctatagc tttcaagcca atatgcaaac
     9061 atgtaataga aagctgtttc tgaagttggg cgtaatgcat atctttgctc aagcttttca
     9121 tcaccgtgct ctgttaccca aaaaacctcg ggagtaaagc cttcaacatg ctctgcttct
     9181 ttaaagaagt tctcttctgg gattaaaact ggcatcataa cttgttggtg tgctgtttca
     9241 tcaaatatgt cttcaaacaa tctaaatatc ttgtttagag taaatgttga ccatggtcta
     9301 tagactacta cgcctttgat attatacctt aaatcagcaa gctctgctct ttgattaata
     9361 tcattaaacc attcattaaa atcttttatt tttagttctt catttgatgc cataatattc
     9421 acatttaact ataatattta ttagaacaat aatttaaaaa taatgatgct ttagctattt
     9481 gccaaacaag ttatccatta actcgttaaa ttcttttatt aacttgcctc tagaaacatg
     9541 tgtgttatat aaagaaacat gcaaatttct aatattttga ataatctcta aatatgcttc
     9601 tcttttctct aatgttgaag tactattaga actgcacttt tctgctaaac tgtttaaact
     9661 ggacaatatt tctttttgaa aagaatttgt tttttcgaat ttaaataatc tttgtatatt
     9721 tgaattaagg ggttctgcta atttattaag gtctattgtt ggactatcta atctgctaag
     9781 cattctttta gaaagacccc agattcttaa ttttgttttt aattttacta tctgtctatt
     9841 aagatagtca tttggatatc ttggatcgac atgtcttaat tttgctgcaa gatcattact
     9901 tagttctgag atttttctat ctgcggtatt ttttgcagtt tttatttttt taaaattttt
     9961 taataaagca tcaggtactt ttgctttctt tggtttatta atggttttac cccagatttc
    10021 tctttgagaa gtagattttt gttttttttt aactaaactc caaatttcta attgttttga
    10081 agttctaatc tttgctatag ttttcaccat tttattaaaa agtgaggctg ccccgatttg
    10141 aacgggggtc aaaagctccc aaagctctta tgataaccaa gctacactac agccccataa
    10201 tatataatat attatagata atatttataa agaataactt ttaagaatta ttgataacta
    10261 ccaggatttg tcattagatc ataaagctct ccagaacatc tgctcatatc ttgaactgca
    10321 gtttcaaaat ccaaactatt atcatataaa cagatcattt ctttatttac ccattctgga
    10381 tttggatttg cagtaaattt ggattttact tcacataggc cctcttttgg gccaattatt
    10441 tcataataat aagtaattgt ttcaaatagc tttgattcta cacttgctga ttgacaactt
    10501 gcaaattttt gtttaaaatc ttcgtagcct tcctcagtta ctacttccat atcaacattg
    10561 acatcaatat gcatttggcc aaaattatcc ccattttcat caactagatt tatatctttt
    10621 tgtgttgcgg aatttacatt ttcggaaacc gatctttcat tattaaaaac aaaaaaatac
    10681 aaacctgcac taattaaaat taatacaata attccaataa ttattattgg caaaatttta
    10741 tttctttttg gcatagtaac agatactggt gcaattatag aattattatt ttcaatattc
    10801 atagcttcat taatttcttc agtagtatag ccttctttaa ttaatgcttc tttaatctga
    10861 tctggactat atccttctgc tgatttattt tctgcccatt tcctaagttc ttcatttacc
    10921 ataatcatca ttattattta gagaaaaaac atttataaac aaagctattt ttaatttact
    10981 tggttattcc aagcttgcca gtgcatttga aagttatcta gatactgctt tgaaatattt
    11041 tggtctttta ttatcaataa ggtttcatca ttattgtttg taccgttgtt tgagaaattc
    11101 atggaaccag ttgtcaggac tttgccatca aaaacacaaa acttgttatg catcaaatat
    11161 ggctggttat cttcaataac ttctatgccc tctgctttta gtttttgata ttctgaatat
    11221 tggcttgtct gctgagtctc tagaattact tttatgtttg ttccggtctt tctttcttca
    11281 actaaggcat caccaaaatc gtcaagcgtg aaactataca tcatacatac tattgatttt
    11341 tctgcatttg aaatttgagt tagcacttgg gttttacaat tttcgtttgg gcagaaataa
    11401 attgagaagt tttcattaaa ggtttttcct aaaacacttg atgactttga gaagtttcca
    11461 agatagaaag aatcaatctt ttcttttaga gtttttgaaa gttctgtatt ttcaaaaact
    11521 aaaatattgt tataactttg agagaagctg ttttctgtaa agtttgttga gcctattaga
    11581 gagatgttat tatcaaaaac acaaaacttg ttgtgcatgt agctnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnacaaga atcttctgga cagaataata caagagacgg
    11761 cgagaccccg ccgccatgtc caagatttag tgggttttgg atttggtttg gcaaaccatt
    11821 atctgcgggc ttgttttcaa agtaaagcaa aataagaaaa ataagaatca aaagaataat
    11881 caaaagaaga aagaagaaaa gataaggatt tttatttttc atgttttgtc tgtgtttggc
    11941 tcaagaacag cttttattct tctaatacct gcagctatgc cttcttcttt aattatctta
    12001 aatttaccaa gctctttagt gttatctacg tgcgggccca tacagatttc ttttgagtgg
    12061 tcgccaatgg aataaacaaa aacagtttct ggatacttgg ttccaaactc tgcttgtgct
    12121 ccgctttcta aagcttcgga aagtagcttt gattctttta taacttctag ctctttggca
    12181 attattttat ttacatcatc ttctattgcc tttatttctt catttgttaa ttttctctca
    12241 aaattaaaat caaatcttag tctttcgttt gtaatattgc tgcctttttg ctttatatct
    12301 ttatctaaaa catttcttaa agactgattt aatatgtgtg ttgtagtgtg ataccttgtt
    12361 gtagtttctg tgtactcagc aaggccgccc ttaaacttct gttctgcgcc tgctctggag
    12421 atatctttgt gatttttctc ttcttcaata aaccctgaaa cgtctacaga tatatttttc
    12481 tctttaccca gttcttcaat catctctatt ggaaagccaa aagactgata aagaagaaac
    12541 gcatcttttc ctgaaagttc tttttgagtt tcagtcattc tattaaattc ttttaatccg
    12601 ttttctaaag ttgtagtaaa tttattttct tctttttcta attcctgtaa aacaaattca
    12661 aattttaatc gcaaatggaa atactcttga tcatataact ccattatttt ttttgaaact
    12721 tttgaacaaa aattattctc tattcctatt aatctgccat atcttattgc tcttcttata
    12781 aatcttctaa gaatatatcc ttgatcagtg ttacttggaa ttgcgcctct agaatctgca
    12841 agaataaaag ttgctgctcg taagtgatct gttattatct ttattgcttt tgtgtttttt
    12901 ttgtcttttg ctagatctaa aacaaaatca taaatttctt ttatcttccc aactttaaaa
    12961 gcatcgtcta aatcatttaa taccattgct actctttctg ttccaaggcc agtatctata
    13021 tttttttgtg ctaattcttc aaattttcct tcctctgtct tattatattc cataaaaaca
    13081 ttattccaaa tctctaccca ggtttcatca tttgggtcaa agacttctgg ggctggttta
    13141 gagttgtcct tccaatagaa tatctctgta tctggcccac aaggtcccga gacacctccc
    13201 gctggccccc accaattgtc ttcaattgct acaattcttt ctttaggtat tccttgttct
    13261 agccaatgaa taattgtttc tttatctatt ggcacatcgt tatttccaga aaagaccgtt
    13321 actgctaatc tgttcttatc aagacctaac cagtctttgc tagtaagaaa ctcataagaa
    13381 taagaaattg attctttttt gaaatagtcg ccaagagacc agtttccaag catttcaaac
    13441 attgaaaaat gtgttgcatc tcctacttgg tcaatgtctg tggttctaat acataattga
    13501 taatcaacta gtcttttgcc tagagggtga ggctgaccta gaaggaaagg aacaagcgga
    13561 tgcatgcctg ctgttgtgaa aagaacggta ttgtcgtttt ctggtatcaa cgatgcctgt
    13621 tcaattactt tgtg
//
LOCUS       DAOOON010000018        12463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_6273_length_12463_cov_11.727031, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000018 DAOOON010000000
VERSION     DAOOON010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6273_length_12463_cov_11.727031"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..546)
                     /locus_tag="PLK55_03085"
     CDS             complement(157..546)
                     /locus_tag="PLK55_03085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOZ35940.1"
                     /translation="MCYFCDIKEKKEMVLFKGKYFFVVYDGFPVTKGHCTVVSNEHKA
                     SFFDLSKEEVDDLYSLILKTKELLEEKHKPDGFNIGVNEGRAAGRTVDHLHIHVIPRY
                     FGDVSDPLGGIRWVIPDKANYRKQPSK"
     gene            complement(594..1520)
                     /locus_tag="PLK55_03090"
     CDS             complement(594..1520)
                     /locus_tag="PLK55_03090"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HOZ35941.1"
                     /translation="MIKMDIKKLISEIKGVGKGKITPMYVYGTENISGYYSKLDLKDK
                     TVLTIAGSGDQAVNAIYYGAKEVTCFDINQLTGYFIDLKINAIKYLNYKEFLEFFGSP
                     SKVGNLNYKLFTKFSKYLNKDAFKFFNDVYVYFKKDGNKIIRSSLFNERSMLLGTLFE
                     MNVYLKTEANYNHCKEILKHKKITFVCEDIFCLDKRLKSRFDILNLSNIPNYFCSRFE
                     YDSKEYKKVFDNLLKLVKKNGILFFYVFSLSTYPNEIAKKTPPFAINKNLIKFCKQFG
                     LSYSFISFNGNGKYVSNIKVKNVRDKIAILKK"
     gene            complement(1617..1757)
                     /locus_tag="PLK55_03095"
     CDS             complement(1617..1757)
                     /locus_tag="PLK55_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35942.1"
                     /translation="MKSKLKSGSKIKYLCSKQAKPRLTILETELAKSWLTKEEDKAWK
                     NL"
     gene            1893..2762
                     /locus_tag="PLK55_03100"
     CDS             1893..2762
                     /locus_tag="PLK55_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOZ35943.1"
                     /translation="MEENKSIAIFENKKIRRLWHNEEWYFSVVNVVEALTDSDNPRNY
                     WNMIKSREQENGVELYTNCVQLKLIAEDGKLRETDCANIEGLFRIIQSIPSPKAEPFK
                     LWLAKLGYQRVQEIKNPELIQKRMVETFKQKGYSDPWIEKRIRGIAIRDELTNEWSKR
                     GITENKEFSILTAEISKATFDMTPSEYKDYKGLKNENLRDHMNDLELIFTMLGESVTK
                     ELTVKEDPKEFPKTKNIAKRGGGVAGVARKVTEKELGRKVSTKENYLTIPEKQKRLML
                     KSKKKKIKYKTKK"
     gene            2943..5780
                     /gene="uvrA"
                     /locus_tag="PLK55_03105"
     CDS             2943..5780
                     /gene="uvrA"
                     /locus_tag="PLK55_03105"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOZ35944.1"
                     /translation="MQKNIIIKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSTLAFD
                     TIYADGQRRYVESLSAYARQFLGMMKKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTV
                     TEVYDYLRLLYARVGVPYCPEHNIPIQPLSAQKIAENILKEYSEEIYLLSPIVQKKKG
                     TYEKLFSDLNKEGFLRVRINGEIARTDEEIKIDRYRMHDIDIVIDRITPSDDRTRLYQ
                     DIEQGLSKSDGLIKVISVKDFEKNKEKAKEKLFSSKMSCPVCGLSFEELQPRMFSFNS
                     PFGACETCSGLGFKMDFDPDLIIPDKTKSMADGAIMIYRNAVDGWRGQHLATLAKHYG
                     FSAMTPIKDLTKEQLKILMYGSEDNIQFNLEGRNGTSSFNYTGTWEGLIPQSQRLYKQ
                     TDSDYRRTEMEKFMRTSPCPDCQGKRLKRKILAVKIADKSIIDITNFSIDFAIKFFKT
                     IKLDNKQKEISNHILKEINSRLEFLEKVGLGYLTLSRSAGTLSGGEAQRIRLATNLGT
                     NLMGVLYVLDEPSIGLHQKDNQKLIDTLCKLRDLGNTVVVVEHDEDTIRQADYVIDMG
                     PGAGIYGGEVVAYGTPKDIEKCKKSLTGQYLAGTEFIHTPKTRRKPNDFVKVIGAKEN
                     NLKNINLEIPTGILTCITGVSGSGKSTLVYETIYPFLMNKFYDSKMEVGKVKDIIIPK
                     SIDKVISIDQSPIGRTPRSNPSTYTKLFDEIRWVFSQSPEAKLRGYDIGRFSFNVKGG
                     RCEACQGDGQIKIEMNFLPDVYVDCEECKGKRYNAETLEILYKKKNIADVLNMSVDEA
                     YSFFENIPGIRRKLQTLVDVGLGYIKLGQSSVTLSGGEAQRIKLTRELSKRDTGNTIY
                     LLDEPTTGLHFHDVKKLIDVLNRLVEKGNTVVVIEHNLDVIKSADYIIDLGPDGGENG
                     GKIVAKGTPEEITKEKKSYTADFVKKILERK"
     gene            5845..6093
                     /locus_tag="PLK55_03110"
     CDS             5845..6093
                     /locus_tag="PLK55_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2683 family protein"
                     /protein_id="HOZ35945.1"
                     /translation="MKALIDLPVEINQTLNIIKAQHNLKNKAEAITLVVEKYSKENLE
                     PELRPEFVKKILAGENKKTYKFKNMDEFDKIIKKHGKK"
     gene            6100..6378
                     /locus_tag="PLK55_03115"
     CDS             6100..6378
                     /locus_tag="PLK55_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HOZ35946.1"
                     /translation="MDYELHEEFIKITNKISAKDPALYTAIRNKVEEIKNCEFLDHYK
                     NLRAPLQEFKRAHITKRFILLFKVDKQNNRIVFRYFDHRDKIYGRTYD"
     gene            6371..7942
                     /gene="uvrC"
                     /locus_tag="PLK55_03120"
     CDS             6371..7942
                     /gene="uvrC"
                     /locus_tag="PLK55_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOZ35947.1"
                     /translation="MINLENIPSSPGCYIYKDVLGKVIYVGKAKNLKKRVSSYFLKKD
                     HDTKTQALVSNISSVDFIVTSNELEALVLENNLIKKYMPKYNIDLKTGQRYAYIQLTK
                     ETFPRLLTLREKPSGKDYNVFGPFVSGQNRLETIRFLNKQFKLRTCKRLPKKPCVRYH
                     IGYCSAPCINNITKEEYSKTIDTVKKILSGKSQEHLPEMKKEMEIYSKSQDFEKALTL
                     RDQISAIEYLAEKQNMERKVNYDQDIINYEIRENTAYVLLFNIDKGLLINKKEFTFDS
                     SLVDISVLDDFLIKYYSNNQKTLPQEIILPEEISLTTKEYLEGLAKKKIAITIPKIGE
                     KKQLLDLCFKNIEITFFRNISKVQALKEKLKLKTEPHVIECFDISHLSGTLTVGSMVT
                     FRGGVPDKSNYRRFRIKTLEKNKIDDFKAIAEVVRRRYLNQTMPLPDLIVIDGGKGQL
                     SSAVEVLKDLRVQNKVQIISLAKRDEEIFRPDNLFPLKLNKKDIALLLLEEIRDEAHR
                     FAINYNKLLRGKEIK"
     gene            complement(7949..8470)
                     /locus_tag="PLK55_03125"
     CDS             complement(7949..8470)
                     /locus_tag="PLK55_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ35948.1"
                     /translation="MQSADIINKIINNKPFIDMDVGIHGRAHANKTLLFSNCLANLVS
                     QGKDIDIDAINISALLHDCGRKNNGKDPYHSINSAELALKFIDDYKIKCNKELVYTCI
                     VRHHPPPNYNDEFKPIESKIVGDADKLDRFRFINQFAPCNPYFFELKESRSLMDIASR
                     INGHKWRSFNNKN"
     gene            complement(8471..9334)
                     /locus_tag="PLK55_03130"
     CDS             complement(8471..9334)
                     /locus_tag="PLK55_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOZ35949.1"
                     /translation="MCEYVGNTFFKLILLFIFIFLFFGLYDKGYLQNLIIVSLVLIAI
                     ILYLIFKLLYRRARLGKIIVLIVVIISAVIVSHYFSSSVFEKTGIQHYITGVETKSAD
                     KQTLSGTTNMIKTIVNSGENILESTGDSIAHSEDDSKVAFDYINELRKENGVNEISWD
                     DKIYDLAKYKVDDMSKRNYFDHVDPDGKCVGSYAKNYGLSYSSDSFAENLFGYSSPYF
                     FDQKESVDSWMDSRGHRYNLLYSGHKRGAIACNSQYCSFIGQGGSSWSCNTGEAGLQF
                     WETVGKQPGEK"
     gene            complement(9505..9738)
                     /locus_tag="PLK55_03135"
     CDS             complement(9505..9738)
                     /locus_tag="PLK55_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOZ35950.1"
                     /translation="MKQLPLLTGKELGKIIVKIGYKYDHTSGSHMTFTNFEEHKKITI
                     PNHGSEPIRPGLLLKIIKKDLGITKEEFFKLLK"
     gene            complement(9747..9956)
                     /locus_tag="PLK55_03140"
     CDS             complement(9747..9956)
                     /locus_tag="PLK55_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOZ35951.1"
                     /translation="MLTTYNIIIEKDKDGWYIAEVLELPGCYSQAKSMDELVKRTKEA
                     IKCHLLESKIEVPVTNFIGIQQLKV"
     gene            10162..12096
                     /gene="uvrB"
                     /locus_tag="PLK55_03145"
     CDS             10162..12096
                     /gene="uvrB"
                     /locus_tag="PLK55_03145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOZ35952.1"
                     /translation="MNKFKLVAPFSPDGDQPSAIKQLSAGLKKKFKKVQTLMGVTGSG
                     KSFTISNVIANYNRPTLVLAHNKTLAAQLYNELKDFFPENKVEYFVSYYDYYQPESYI
                     PQKDLYIEKNTKINDKIEQMRLSATTSLVSRNDVIVVASVSCIYGLGNPETFKEKGFT
                     IEKGQKLKRTELLRQLIDIQFERNDIELEPGRFRVKGDVIDLSPGYTQNFIRIELFGE
                     TVDRITEMDKITNLKISDFDKFYVHPAKQFVITKESLQPAIASIREELEARLPELQEL
                     ERHRLKQRTNYDIEMIEETGYCNGIENYSRHFDGRKAGERPFCLLDYFPDDFLLVIDE
                     SHQTIPQLHGMYAGDYSRKKTLVDYGFRLPSAYDNRPLKFKEFEKFMDAVIFVSATPA
                     EYETKQSFQVVEQIIRPTGLVDPPVFIKPSKNQIEDLTENIRKTIKKGNRCLVTALTK
                     KLAEELADFLSRQGIKTRYLHSEINTLERTEIIRELRLGKFDVLVGINLLREGIDIPE
                     VSFIGIIDADKEGFLRDQRSLIQIIGRAARNSESYVHLYADKMTESIKGAVKETERRR
                     KLQLDYNQSHGIIPKTIIKPIREKEIDEIKDILHIPKPEIPNLIIELEKQMVEASDKL
                     DFETAIRLRDKVKELKKREI"
     gene            12135..>12463
                     /locus_tag="PLK55_03150"
     CDS             12135..>12463
                     /locus_tag="PLK55_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35953.1"
                     /translation="MKLGLPRLEKVQKYITYGIILSLIVAVVFEIINKNHEFLFITVL
                     SIILCLVPFFIQRRYKIQLPSEIQLATVLFIYSGIFLGEVNNFYVKYWWWDSLLHTFS
                     GFALGLVV"
BASE COUNT         4612 a         2026 c         1928 g         3897 t
ORIGIN      
        1 cattttctat catttgcaaa tttcattgca gaattttcta aatcttttat tgtttttgac
       61 ataatttcac caattactat acaaagatta attatagttt aaaactcttt ttatatcttc
      121 cttttactac agaagaatta ctactgactt ctacgactac ttacttggtt gctttctgta
      181 gtttgctttg tctggaataa cccacctgat tcctccaagg ggatcagaaa catctccaaa
      241 gtatcttgga attacatgta tgtgaagatg gtctactgtt cttcctgcag ctctaccttc
      301 atttacgcct atattaaatc cgtctggctt gtgcttttct tctaataatt cctttgtctt
      361 aagtataaga gaatataaat catccacctc ttctttactt aaatcaaaaa aagacgcttt
      421 atgctcatta gaaacaacag tacaatgccc tttagtaact ggaaaaccat catacactac
      481 aaagaaatac ttacctttaa acaacaccat ttctttcttt tccttaatat cgcaaaaata
      541 acacataatt atctcacaat agaactttag tttaaactct ttaaatatat ttgttatttc
      601 tttaatatgg caatcttgtc tcttacgttc tttactttta tgttggaaac gtatttgccg
      661 tttccattaa aactaataaa gctataggat aacccaaatt gcttacaaaa tttaataaga
      721 tttttattta ttgcaaaggg tggagttttt ttagcaattt cgttaggata agtggatagg
      781 gaaaaaacat agaagaataa tatcccattc ttcttcacca actttaaaag attatcaaaa
      841 actttcttgt attctttaga atcatattca aacctggaac agaagtaatt aggtatattt
      901 gataggttta atatatcaaa tctagacttt aacctcttat ccaaacaaaa aatatcctca
      961 caaacaaatg ttatcttctt gtgctttaat atttctttac aatgattata atttgcttca
     1021 gttttaagat aaacattcat ttcaaacaaa gttccaagaa gcattgatct ttcattaaat
     1081 aaacttgaac gaattatctt atttccatcc tttttaaaat aaacataaac atcattaaaa
     1141 aacttaaatg catccttatt tagatactta gaaaacttag taaataattt ataattcaga
     1201 ttaccaacct tacttggact tccaaaaaac tcaagaaact ccttatagtt tagatacttt
     1261 attgcattta ttttcaaatc aataaaataa ccagtaagtt ggttaatatc aaagcaagta
     1321 acttcttttg ccccataata aatagcatta actgcttgat ctccagaacc agcaatagtt
     1381 aaaacagttt tatcttttaa atcaagttta ctataatatc cagaaatatt ctctgttcca
     1441 taaacataca taggagtaat cttaccttta cctactcctt ttatttcaga aattaatttt
     1501 ttaatatcca ttttaatcat aatatttatt attcaataac tatataaata attagttgag
     1561 tgttattgtt ctataataat cagaaaaagg aaaaggaact actactatct gcccttttac
     1621 aaattcttcc atgctttatc ttcttctttg gttagccaag actttgctag ttctgtttct
     1681 aatattgtta gtcttggttt tgcctgcttt gagcaaagat attttatctt tgaaccagat
     1741 tttagttttg atttcataaa tatctattac cttaagatgt atataaagct attctttgat
     1801 ttttttgtaa atttgaagta agggggtcgg acagctaccc ccccgaaaac aacccctgct
     1861 tctactacta tatattattg gcgatcatta aaatggaaga aaataaatca attgcaattt
     1921 ttgaaaacaa gaaaataagg agactctggc ataacgagga atggtacttc tctgttgtta
     1981 atgtcgttga agcattaact gatagcgata atcctcgaaa ttactggaat atgataaaat
     2041 ctagagaaca agagaatgga gttgaactgt acacaaattg tgtacagttg aaattgatag
     2101 cagaagatgg aaaattaaga gaaacagatt gtgcaaatat cgaaggtttg tttagaataa
     2161 tacaatcaat tccatctcct aaagcagaac ctttcaaact atggctagca aaacttggct
     2221 atcaaagagt acaagaaatt aaaaatccag aacttataca gaaaagaatg gttgaaacct
     2281 ttaaacaaaa gggatattct gatccctgga ttgaaaaaag aataagaggt attgcaatta
     2341 gagatgaact aacaaatgaa tggagcaaaa gaggaataac tgaaaataaa gaattttcaa
     2401 ttcttactgc agaaatatca aaagcaacct ttgatatgac tccttctgag tataaagatt
     2461 acaaaggact aaaaaatgaa aatctaagag accacatgaa cgacttagaa ctaatattta
     2521 caatgcttgg agaatcagta actaaagaac ttacagttaa agaagaccct aaagaatttc
     2581 caaaaacaaa aaacattgct aaaagaggcg gcggagttgc tggagttgca agaaaagtaa
     2641 ctgagaaaga acttggtaga aaagtatcca caaaagaaaa ttatttaaca attcctgaaa
     2701 aacagaaaag attaatgcta aaatcaaaga aaaagaaaat aaagtataaa actaagaaat
     2761 aatcatcatc attttgttga cgccaacaaa atgatataag gggtcgaatt cgaccccttc
     2821 aaacccatcg aattcgaggg gtttgagaga aaaaagcatt acctttttaa cagctttatc
     2881 acttaattat atattgtagt caagcatttt taagatttta aaaaagatgg agtaataccc
     2941 ctatgcagaa aaatattatc ataaaaggtg ccagagagca taacctaaaa aacatagacg
     3001 tagagatacc tagagataag ctggtagtga ttactggctt atctgggtca ggaaaatcaa
     3061 ccctggcctt tgataccatc tatgctgatg gccagagaag gtatgtggag tctttgtctg
     3121 cttatgctag gcagttttta ggaatgatga agaagccaga cgtggattct atagaagggc
     3181 tatctccagc aatctcaatc gaacaaaaga caacctccaa aaaccctcgt tcaactgtag
     3241 gaacagtaac tgaggtttat gactacttaa gattacttta tgcaagagtt ggtgtaccat
     3301 attgtcctga acataatatc ccaatccaac cactttctgc acaaaagata gcagagaata
     3361 tacttaaaga atattcagaa gaaatctatc ttctttcacc aattgtccag aagaaaaaag
     3421 gaacctatga aaagcttttc tctgatctta acaaagaagg atttttaaga gtaagaataa
     3481 acggagaaat agcaagaaca gacgaggaaa taaagattga tagatataga atgcatgata
     3541 ttgatattgt tattgataga ataacaccct ctgacgacag aacaagacta taccaagaca
     3601 ttgaacaagg tttaagtaaa tcagacggac taataaaagt aatatctgta aaagactttg
     3661 aaaagaacaa agaaaaagca aaagaaaagc ttttctcctc aaagatgtct tgccctgttt
     3721 gtggtctttc ctttgaagaa ctacaaccta gaatgttttc cttcaactct ccttttggtg
     3781 cttgtgaaac ttgttctggt ctaggcttta aaatggactt tgaccctgac ttaattattc
     3841 cagacaaaac aaaatccatg gcagacggag caataatgat atatagaaac gcagtggatg
     3901 gttggagagg ccaacaccta gcaactcttg ccaaacacta tggcttttca gcaatgacac
     3961 caatcaaaga cttaacaaaa gaacaactta agattttaat gtatggttca gaagacaaca
     4021 ttcagtttaa ccttgaagga agaaacggaa cttcaagttt taactataca ggaacttggg
     4081 aaggacttat tcctcagtcc caaagacttt acaagcaaac agactcagac tatagaagaa
     4141 cagaaatgga aaaattcatg agaacctctc cttgccctga ttgccaaggt aagaggttga
     4201 agagaaaaat cttagcagtc aagattgcag acaaatcaat tattgatatc actaactttt
     4261 caattgactt tgcaattaag ttctttaaaa caattaagct tgacaacaaa caaaaagaaa
     4321 tatctaatca catactaaaa gaaatcaact ctcgtttgga gttcttagaa aaagttggtc
     4381 ttggttacct aactctttca agatctgcag gaactctttc aggaggagaa gcacaaagaa
     4441 ttcgtttggc aacaaatctt ggaactaacc ttatgggcgt tctttatgta cttgacgaac
     4501 catcaattgg tcttcaccaa aaagacaatc aaaaacttat tgatactctt tgtaagctta
     4561 gagatcttgg taacacggtc gttgttgttg aacatgacga agacactata agacaagcag
     4621 actatgtaat tgacatggga ccaggggcag gaatctatgg cggagaagtt gtggcttatg
     4681 gtacgcctaa agacatagag aaatgtaaaa aatcattaac tggccaatac cttgcaggaa
     4741 cagaattcat acatactcca aagacaagaa gaaaaccaaa cgattttgtt aaagttattg
     4801 gagcaaaaga aaataatctt aaaaatatta atcttgaaat accaacagga attttaacat
     4861 gcattactgg agtttctggt tctggtaaat cgactcttgt ctatgaaacc atctatcctt
     4921 ttttgatgaa caaattctat gattcaaaaa tggaagttgg aaaagtcaaa gacatcatta
     4981 ttccaaaatc catagacaaa gtaatatcta ttgaccaatc accaattgga agaacaccaa
     5041 gatccaatcc ttcaacatac acaaagttgt ttgatgagat tagatgggtg ttttctcagt
     5101 ctcctgaagc caaacttaga ggttatgata ttggaagatt ttcttttaat gtcaagggcg
     5161 gaagatgcga ggcttgtcaa ggagatgggc agattaagat tgaaatgaac ttccttccag
     5221 acgtctatgt tgactgtgaa gaatgtaaag gcaagagata taatgctgag actcttgaga
     5281 ttctttacaa gaagaaaaat atagcagatg tgctaaacat gtcagtagac gaagcgtatt
     5341 ctttctttga aaacattcct ggcattagaa gaaagctaca aactttagtg gacgttggtc
     5401 ttggttacat taagcttgga caaagttcag taactctttc aggaggagaa gcgcagagaa
     5461 tcaagctaac tagagaactt tcaaagagag acacaggaaa cacaatttat ctccttgacg
     5521 agccaacaac tggtcttcat ttccatgatg ttaagaagtt gattgatgtt ttaaacagac
     5581 ttgtagagaa aggaaacaca gttgttgtta ttgaacataa ccttgatgtt attaaatctg
     5641 cagactacat cattgacctt gggccagacg gtggagaaaa tggaggaaag attgtagcaa
     5701 aaggaactcc tgaagaaatc acaaaagaaa agaaaagcta tactgcagat tttgtaaaga
     5761 agatacttga gaggaaataa gcatatatta ataaatgtta ataaccatta atatgtatat
     5821 acaactatta atgggaggga gaaaatgaaa gcattaattg acttgccagt tgagattaac
     5881 caaactctaa atatcataaa ggcacagcat aatctaaaga ataaggcaga agcaataact
     5941 cttgttgttg aaaaatattc taaagaaaac cttgaaccag aattaagacc tgagtttgtt
     6001 aaaaaaatac ttgctggtga aaacaaaaag acttacaaat tcaaaaatat ggatgaattt
     6061 gataaaatca ttaaaaaaca cggcaaaaaa tgataattta tggattacga attgcatgag
     6121 gagtttataa aaataacaaa caaaatatct gcaaaagatc cagcactata tactgcaatt
     6181 cgtaacaagg ttgaagaaat caaaaattgc gaattcttag atcattacaa gaatctcaga
     6241 gctccacttc aagaatttaa aagagcacat ataactaaga gattcatttt attattcaaa
     6301 gtagataaac aaaacaatag aattgtcttt agatattttg atcatagaga taaaatttat
     6361 ggaagaacat atgattaact tagaaaacat accaagtagt cctggttgtt atatctataa
     6421 ggatgtcctt ggtaaagtta tctatgtagg aaaagcaaaa aacctaaaga aaagagtatc
     6481 ttcttatttc ttgaagaagg atcacgacac aaaaactcaa gcacttgtct ctaatatctc
     6541 ttctgtggat tttattgtaa cttctaatga gcttgaagct cttgttcttg agaacaacct
     6601 aatcaagaaa tatatgccaa agtataatat tgatttaaag acagggcaaa gatatgctta
     6661 tatccaattg acaaaggaaa ctttccctcg tctcttgacc ttaagagaaa aaccaagcgg
     6721 gaaagactac aatgtctttg ggccttttgt ttctggacag aacagattag agacaattag
     6781 gtttcttaac aaacagttta aactaagaac atgtaagaga cttccaaaaa agccatgcgt
     6841 gagatatcac ataggttatt gttctgctcc ttgtattaac aacataacaa aagaagaata
     6901 ttcaaagaca attgacacag taaagaaaat cctttccgga aaatcacaag aacatctccc
     6961 tgagatgaaa aaagaaatgg aaatctattc caagagccaa gattttgaaa aagccctaac
     7021 tcttcgagac caaatctcag caatcgagta ccttgcagag aaacagaaca tggaaagaaa
     7081 ggttaattac gatcaagaca ttataaatta tgaaattaga gaaaacacag cttatgttct
     7141 tctcttcaac atcgataaag gactattgat aaacaaaaaa gaatttactt ttgattcatc
     7201 tcttgtggat atttctgttc ttgatgattt tttaatcaag tactattcaa ataaccaaaa
     7261 gactttgcct caagaaatta ttcttcctga agaaatctcc ctgacaacaa aagaatatct
     7321 tgaaggtttg gcaaaaaaga aaatcgcaat cacgattcca aagattggag agaaaaaaca
     7381 gcttcttgac ttgtgcttta agaacataga gattactttc tttagaaata taagtaaggt
     7441 ccaagcactt aaagaaaaac taaaactaaa aacagaacca catgtcattg agtgttttga
     7501 tatttctcat ctttcaggaa ccctcacagt tggctcaatg gttaccttta gaggtggcgt
     7561 cccagacaaa agcaattatc gtagatttag aattaaaact cttgaaaaaa ataagattga
     7621 tgatttcaag gcaattgcag aagttgtaag aagaagatat cttaaccaaa caatgccact
     7681 tccagacttg attgtcattg acggagggaa aggtcagctc tcctcagcag ttgaggtcct
     7741 caaagacctg cgtgtccaaa ataaagtgca aattatctct cttgccaaga gagatgaaga
     7801 aatctttaga ccagataacc tgtttccact caagctaaac aaaaaagaca tcgctcttct
     7861 tctcctagaa gagataagag acgaagcaca cagatttgca atcaactata ataaactatt
     7921 aagagggaag gaaattaaat aacgataatt aatttttatt gttaaatgat ctccatttat
     7981 ggccgtttat tctagatgct atgtccatta aagaccttga ttcttttaat tcgaaaaaat
     8041 acggattaca tggcgcgaat tgatttataa atctaaatct atctaattta tcggcatcac
     8101 caactatttt agattctatt ggtttaaact catcattata gtttggtggt ggatggtgtc
     8161 ttactataca agtatatact aattctttat tgcattttat tttataatca tctatgaatt
     8221 ttaatgctaa ttctgctgaa tttattgagt ggtatgggtc ttttccatta ttttttcttc
     8281 cacaatcgtg caataatgcc gaaatattta ttgcatcaat atctatatct ttaccctgcg
     8341 aaactaaatt tgctaaacaa ttagaaaaca aaagggtttt atttgcatgt gctcttccat
     8401 gaatcccgac atccatatct ataaagggtt tgttatttat tattttattt attatatctg
     8461 cactttgcat ttatttttca ccaggttgct ttcctactgt ttcccaaaat tgcaatccgg
     8521 cttctccagt attacaactc caactgcttc cgccttgccc aataaacgaa caatattgag
     8581 aattacatgc tattgctcct cttttatgac ccgaatacaa taaattatat ctgtgtcctc
     8641 tactatccat ccaactatcc actgactctt tttgatcaaa aaaatatggg gaactatatc
     8701 caaataaatt ttcggcaaat gaatcagaag aataagacaa tccataattc ttagcataac
     8761 tgcctacaca tttaccatcc ggatctacat gatcaaaata attccttttt gacatatcat
     8821 caactttata ttttgctaaa tcataaattt tatcatccca ggagatttca tttacaccat
     8881 tttctttacg tagttcgttt atataatcaa atgcaacttt tgaatcatct tctgaatgag
     8941 ctatagaatc tcctgtagac tccaatatat tttcaccgct atttactatt gttttaatca
     9001 tatttgtggt tccactaagt gtttgtttat ctgcagattt ggtttctact ccggttatat
     9061 aatgttgaat tccagttttt tcaaagacag aacttgaaaa ataatgagaa acaataactg
     9121 ctgaaattat tacaactatt aaaacaatta ttttacctaa tcgggctctt ctatataata
     9181 atttgaaaat taaatatagt ataattgcaa taagaactaa agacacaatt atcaaatttt
     9241 gtaaataacc tttatcatac aaaccaaaaa acagaaaaat aaagataaac aataaaatta
     9301 atttaaaaaa tgtgttccca acatattcac acatataatc taataaaatt ttaaatttag
     9361 aactattaaa tttgtgttgg ttcatattga ttatttaatc aaaaacttat ttaaatctat
     9421 tttatcttaa atttgccgtc gccgattcca aacaaggtgc ctttcccatt taaatattat
     9481 taaaatacat ttctaaatag ctaactattt taataattta aaaaactctt ctttggttat
     9541 ccctaagtct ttctttatta tctttagaag tagccctggt cttattggtt ctgaaccgtg
     9601 gtttggaatt gttattttct tgtgttcttc aaaattagta aaggtcatgt gacttccact
     9661 agtgtggtca tatttataac ctattttaac gattatcttt ccaagttcct taccagttaa
     9721 caaaggtaat tgtttcaaag aggtctttac acctttaatt gttgtattcc tataaaatta
     9781 gtaactggaa cctctatctt agattcaagt aaatgacatt taattgcttc ttttgttctc
     9841 ttaactagtt cgtccatact ttttgcttga gaataacaac caggtaattc taatacttca
     9901 gcaatatacc aaccgtcttt gtctttctca ataattatat tatatgtagt taacattttt
     9961 ttacccttct tcatatagta attaaactag taaatactta aaaatatatg taaattataa
    10021 tttcacacct atataaatct acttcttaga ttctttggaa ttatactaaa aacagtgttt
    10081 ggtttttcaa atatacaaaa gtcctttgtt ttttccttta aatatattaa aatacatttc
    10141 taaacttaat tatatattgt catgaacaaa tttaaactag ttgcaccgtt ttctccagat
    10201 ggagatcagc ctagcgcaat taagcagcta tcggcagggc taaagaagaa gtttaagaag
    10261 gtgcagactc ttatgggagt tacgggctca ggaaaaagtt ttacaatcag taatgttatt
    10321 gctaactata atcgcccgac cttggttctt gcccacaaca agacacttgc tgcacaacta
    10381 tacaatgaac taaaggattt ctttcctgaa aataaagtag aatattttgt ttcatattat
    10441 gattattatc aacctgaaag ttatattcca caaaaagatc tttacattga aaaaaacaca
    10501 aaaataaatg acaagattga acagatgaga ttgtctgcaa caacttctct tgtctcaaga
    10561 aacgacgtca ttgtagttgc ttctgtttct tgtatctatg gcttaggaaa cccagaaact
    10621 ttcaaagaaa aaggttttac tattgagaaa ggacaaaagc taaaaagaac agaactatta
    10681 agacaattaa ttgatattca atttgaaaga aacgacatag aactagaacc aggaagattt
    10741 agagtcaaag gagatgttat tgatctttct ccaggatata ctcaaaattt tataagaata
    10801 gaattgtttg gagaaactgt agacagaatt acagaaatgg ataaaattac aaatctaaag
    10861 ataagcgact ttgataaatt ctatgttcac cctgctaagc agtttgttat tactaaagag
    10921 tctcttcagc ctgcaattgc ttccataaga gaagaactag aagcaagatt accagaacta
    10981 caagaactag aaagacatag attaaagcaa agaactaatt atgatattga gatgatagaa
    11041 gaaacaggat attgtaatgg catagaaaat tattctagac actttgatgg aagaaaagca
    11101 ggagaaagac ctttttgtct tcttgattac ttccctgatg atttccttct agttattgat
    11161 gaatctcacc agacaatacc acaactacat ggaatgtatg ctggagacta ttcaagaaag
    11221 aaaactcttg ttgattatgg ttttagactt ccttctgctt atgataatcg tccattaaag
    11281 tttaaagaat ttgaaaagtt catggatgcc gttatctttg tttctgcaac tcctgctgag
    11341 tatgaaacta aacaatcttt ccaagttgtt gaacaaataa ttagaccaac aggccttgtg
    11401 gaccctccag tatttattaa accatcaaag aaccagattg aagacctaac agaaaatatt
    11461 agaaaaacta taaagaaagg aaacagatgt cttgttactg ctctaacaaa aaagcttgca
    11521 gaagaacttg ctgatttcct ttctcgccaa ggaatcaaaa ctagatatct tcactcagag
    11581 attaacactc ttgaaagaac agagattatt agagagttga gacttggaaa gtttgatgtt
    11641 cttgtaggaa ttaatctttt acgagaagga atagatatcc ctgaagttag ttttattggt
    11701 atcattgatg ctgacaaaga aggtttctta agagatcaaa gaagcctaat acagattatt
    11761 ggaagagcag caagaaactc agaatcatat gttcatctct atgctgacaa gatgactgaa
    11821 tctattaaag gcgcagtaaa ggaaacagaa agaagaagaa agcttcaatt ggattacaat
    11881 caatcacatg gtattattcc aaagaccatc atcaagccaa taagagaaaa agaaattgat
    11941 gaaattaaag atatcttaca tatcccaaaa ccagaaatac caaacctaat aatagaacta
    12001 gaaaagcaaa tggttgaagc ttcagataaa cttgattttg aaactgcaat aaggttaaga
    12061 gataaagtaa aagaactaaa gaaaagagaa atttagaaat atttatataa tctaataaac
    12121 aataaatatt tactatgaaa ctaggattac caagactaga gaaagttcaa aaatatatta
    12181 cttatggaat tatactttct ttaatagttg ctgtggtttt tgaaataatt aataaaaacc
    12241 atgagtttct ttttattact gttctttcta taatcttatg cttagtgcct ttttttattc
    12301 aaagaagata taagattcag ttgccttctg aaatacaatt agctacagta ttatttattt
    12361 attctggaat cttcttagga gaagttaata atttctatgt caagtattgg tggtgggatt
    12421 cattacttca caccttctct ggatttgctt tgggccttgt agt
//
LOCUS       DAOOON010000019        11817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_6733_length_11817_cov_20.545996, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000019 DAOOON010000000
VERSION     DAOOON010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_11817_cov_20.545996"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..361
                     /locus_tag="PLK55_03155"
     CDS             107..361
                     /locus_tag="PLK55_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35954.1"
                     /translation="MKVDKHTKLGKIIKLKGIAKILTKHRVPCVTCPLAKMEMDSLEI
                     GHVCSMYGLNEKELLLDINRILKHKDKIEKPKPKRKVKAI"
     gene            complement(358..1017)
                     /locus_tag="PLK55_03160"
     CDS             complement(358..1017)
                     /locus_tag="PLK55_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35955.1"
                     /translation="MMKKIILSFLIFCGLLFSATPIFADSISEGYKSVKYCIQFTNMA
                     DFSDYSLFKVCDTTMPSVAEMTDPNGCYGMYKFCNFKVCAIKKEKMFEELRAEINDYD
                     REDITTKIVNNPDFVCKDLNYRPVSQVRDSDPTTSITDVYSLPDFSISDSQVIKEKVV
                     YNQENSKVAEEIPYTSDTRPISKYSQNTPLTVYVLVGGIILLIILLSIIQIKKLKRKS
                     I"
     gene            complement(1030..2379)
                     /locus_tag="PLK55_03165"
     CDS             complement(1030..2379)
                     /locus_tag="PLK55_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOZ35956.1"
                     /translation="MKDLNNFFEPKSVCLIGASDKKGSVGNTIAKNLSGFKGKVYFTN
                     PRLSGKKLLDKKVFSSITEISEKIDLSVVATPAAITFEVVKDIAKSKCKNILMVTSGF
                     SEIGRTDLTEKIKKIILKEKINLIGPNCLGLLNLSNHLDATFNSRDKYDLPKSGKVSI
                     ITQSGALGIALLDLASKQRLEINKFVSYGNALDIDEIDLLEYFGKDKDTDIILCYIEG
                     VKDGKKFYETLKKVTQKKKVIVLKGGISEKGTTAVKSHTAAIAGSIKVFSTALRQAGA
                     IEVKTIKEMFDISRFFSWYSVDNIKDSQIITNGGGFGVLTTDQLDINNLKLSEITKET
                     TDKIKKIIPSYATIGNPIDLTGDADDKRFLQAIYLCAKDKNTNSIIVLMLLQLPSISN
                     KIPEEIAKLKANTKKPIMVVTIGGNKTEKKIEEFEKRKVLCFRDPFELSSILKYLKK"
     gene            2480..3037
                     /locus_tag="PLK55_03170"
     CDS             2480..3037
                     /locus_tag="PLK55_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOZ35957.1"
                     /translation="MKDNFEIMIYGRAGLGAKTCAQLIADSALLDGKVVQAFPEYGPE
                     RRGAPVRSFVRISNKQIRVHEPITHPDLVIVIDCGLFNVLPGLSNFDCDYIINSDNDK
                     AFFKKFIKNEKKINVIDGSKISMELLKINNPNAVLIGAFVNLFGVIKYSSVEESIKKE
                     FEKKGKADIIKPNLLCLKKGFEHFK"
     gene            3043..3318
                     /locus_tag="PLK55_03175"
     CDS             3043..3318
                     /locus_tag="PLK55_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOZ35958.1"
                     /translation="MAETKKIGGVLPGGTAQVNKTGSWRSLRPEWDSKKCIHCMLCFN
                     ACPEQCINANKDAKGNIKRQETNLDYCKGCGICAEICPVKCIKMVKE"
     gene            3315..3890
                     /locus_tag="PLK55_03180"
     CDS             3315..3890
                     /locus_tag="PLK55_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ35959.1"
                     /translation="MNSFTEDIEKIETIIKVKESTRFGIVGDRFESSAEHTFSCILLA
                     QYFIKKIPYKLDELKVIKLLSYHDIQNVFFKDINLSDDSWSNKKDLEKVAFKKTINSL
                     PSAIAGDYEKNYLEFEGLLTTESRFAKAIDVLEPVIHAYNQKKHWKEGKWTESKLRMH
                     KEKYLDEFPVLMKFFNDFVDYANQNDYFYKE"
     gene            3902..>4521
                     /gene="porA"
                     /locus_tag="PLK55_03185"
     CDS             3902..>4521
                     /gene="porA"
                     /locus_tag="PLK55_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOZ35960.1"
                     /translation="MKTTKLGLSGGEAIAYALKQINPEVFAMYPITPQTPIIESYSKY
                     SANGLVDTEIIRTESEHSALSATIGASASGVRAVTATASQGLLYMFEALSVASGLRLP
                     IVMPVANRAISAPINIHCDHSDSMSALDQGWMQVYCESAEEAYDMTLFSLRLAESVSL
                     PIMVCIDGFYTSHNMEMINVYDDLSVKKFIGEFKPNYNLLDTDKPIT"
     assembly_gap    4522..4621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4622..4879
                     /gene="porA"
                     /locus_tag="PLK55_03190"
     CDS             <4622..4879
                     /gene="porA"
                     /locus_tag="PLK55_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOZ35961.1"
                     /translation="YEEYSKALTKAKKIIVLERTEGAGSFPPLYKDICLTVMNTTNKP
                     EIISLVFGLGGRDIYQKDIEELLVSVISGKKLKSRYIGIKE"
     gene            4883..6919
                     /locus_tag="PLK55_03195"
     CDS             4883..6919
                     /locus_tag="PLK55_03195"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOZ35962.1"
                     /translation="MNSKELAIKLLEKKAMVSGYRTCAGCPIAIICKTVIAASDKPVV
                     ASSATSCAEVTTTVYPQTAWNIPWIHSLFANSAATISGVECAYKAQKKKGKIKKDINF
                     LVIAGDGGSFDIGLQSLSGALERGHDFVYLCYDNEGYMNCLSKDSLIYTESGLKKITE
                     IKVGEMVYAFDQKTHKLVLKKCSGVFDNGIKKVYEIETFHHNIKATGNHPFLVANKLS
                     RGKPNTLIWKTLEELKKGDQVITLKKLKGNKPFTFEKITLSKKGDYKVNSINEVKIPE
                     KSTTDLMLFLGLYVGDGWIRDKGEIGFALPEGTEGREKCIATCKKVFGLKTITTDKTY
                     IYMNSINITKFINSLGFNKGAKNKIIPSWVYTIPDEQKEAFIEGLLLSDGYIHKKKKD
                     SCRYLSASKELILRLRLLLQTLNYRAGKITVQKKEKGTFVVYRPLLKDAIYYSVCFSK
                     KYPWNTTKYPTQYKYQNFLIGNENFDTEIIKNIKEIGKEQTFDLRVEGEHNFIADGIV
                     VHNTGNQRSSATPKGAVTSTTPSGKISFGKLEFSKDLTKIAAAHNIPYVAQINSHNQI
                     DMFEKAKKAFEVNGPAVLVSFAACPTNMKADSKYTIKISKLATETCFWPLYEVINGKV
                     IINYKPTNKLPIEEFLKTQTKFKDVLKPENKKVLQEIQAEVDKRWAELLKLEKL"
     gene            complement(6914..8092)
                     /locus_tag="PLK55_03200"
     CDS             complement(6914..8092)
                     /locus_tag="PLK55_03200"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOZ35963.1"
                     /translation="MNILVINAGSSSLKFQYINPDNGEVLIKGLYDGLNRTDGKSCLR
                     VLTIGSKSTQETEIKTHRQAIEDMLSVLIERKIIENLFDIRFIGHRVVHGAEEYSKTT
                     EITEAMLLHLKKISFLAPLHNPVNIECIEILKEKLPKARQFAVFDTAFHQTIPEKIYL
                     YGLPKELYDKFKIRKYGFHGISHKYVSKKATEMLGKDYNNLKIITCHLGNGQSICAIN
                     KGKSFDTSMGFTPVDGLPMGTRSGSFDPEIILFLLENGYSKEQIKKMINKESGLFGIS
                     QISSDHRQIEDLSIAGDKNAKLANDILTTRIVSIVGSYIAEMNGIDVIVFTGGIGEKS
                     AYLRELVLDSFSYLGLKIDKEANKKHDTIISTKDSKVIAMVVPTNEELEIAIETKENL
                     "
     gene            8213..>8433
                     /locus_tag="PLK55_03205"
     CDS             8213..>8433
                     /locus_tag="PLK55_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOZ35964.1"
                     /translation="MTKKIIIGIQMLFVAFGATVLVPLLVGIDPAVALFTAGCGTLLF
                     HFITKGKVPVFLGSSFVFIAPIIKATELYG"
     assembly_gap    8434..8533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8627..8833)
                     /locus_tag="PLK55_03210"
     CDS             complement(8627..8833)
                     /locus_tag="PLK55_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35965.1"
                     /translation="MPRGKLTTTGSVLGKNRNAHKKTNHEQKKDSNAKKRITAEEYRK
                     HKREIDQLLRGLKGKSNLREAELD"
     gene            complement(8871..10031)
                     /locus_tag="PLK55_03215"
     CDS             complement(8871..10031)
                     /locus_tag="PLK55_03215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOZ35966.1"
                     /translation="MAAKKETKAGKKHEKKGLDKYETETTKDIEQRTLQIAKDIVDQI
                     KGNRHPTFTTKVRGLSNVYFDEDKKYIQLGDKKTVRPFLNLGAARKFMQTMLVNDKCN
                     EYLKEGKTASIREIYYELKRTVANTKENTFEGQGESDEVIVDVEHAANTIREKLGLHA
                     EAKGSLYGDITLKDLKHDNDIFNCNSLGRGGWSIMSRIEPEEIEIVDVNADFVLVIET
                     AAMYNRLIEERYARQHNAILIGTGGQGARGARRLVHRLRYEYDLPVIVFTDGDPYGWY
                     IYSVIKSGSMALAAHSKYMAVPDARYVGMTITDVETYGLQNVTEKMKEGDIKRAKELM
                     NYDWFKSKEWQSELKTAIDKKIRIEQQALANKSLDFVARKYLPEKIEKEIFL"
     gene            complement(10042..11739)
                     /gene="top6B"
                     /locus_tag="PLK55_03220"
     CDS             complement(10042..11739)
                     /gene="top6B"
                     /locus_tag="PLK55_03220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOZ35967.1"
                     /translation="MAEKEAKETIRKEVGAEELFTEFKEHSVADFFKKNKQMLGLSGK
                     QRTMTTIIHELVTNSLDACEEAGILPEIEVTISELGSEYYEISVVDNGPGIPEDKLAS
                     AMGKLLAGTKFHRLVQTRGQQGIGVSGIILLSQMTTGKPTKLISGTNKGKAISMEMSI
                     DSKTNTPKVSEKHYLENKFKGLAVKCKFKDIIYKKGQSSVDEYLRRTAISNPHLNLSF
                     QDPLGEKIKYTRTINKLPNIPTAVKPHPIGVSVDEMLTYARHSAGSRKINSFLMTTFD
                     RFGQTSVDNVQKLVHFDLNKDPRAMTWEEAEDLIKAIKQTKFIAPSTDNLVPIGEEQI
                     KKSLKSIVEPEFLSVVTRKPTVYQGGYAFQVEAAVAYGGKSGRLISVEKTIDKKQTII
                     EEVRSSEIIRYANKAPLLFDAGGCSITKTVESVDWKRYGVSNFDTSPITIFVNLISVH
                     IPYTSAGKSAVSDDSDIVNEIKLALMDVARRVGIYIIEKKREEEKKEKKKVFAKYAPY
                     VVDALIHTTGEKNRDLLLKNLNNMVLEKLRLEEQGIKTSDDEVVDDDSVVEDEEFEE"
BASE COUNT         3807 a         1887 c         1790 g         4133 t
ORIGIN      
        1 gactattatt ctaaacatgg aaagaaaaca aaaaaaataa aaacaaatgc atgtacacat
       61 tgttgtacaa ataaggtttg taatcataaa aaagggaggt agatatatga aagtagataa
      121 acacacaaaa ctaggaaaaa taataaaatt aaaaggcatt gcaaaaattc taactaaaca
      181 tcgtgtgcct tgcgtgactt gcccactggc aaagatggag atggattctt tagagattgg
      241 tcatgtttgt tcaatgtatg gactaaacga gaaagaactt cttttagata taaaccgaat
      301 tttaaaacac aaagacaaaa tagagaaacc aaaacctaaa agaaaagtaa aagcaattta
      361 gatgctttta cgttttaatt ttttaatttg tattattgat agtaaaataa tcaataaaat
      421 aatacccccg acaagaacat atacagttaa tggcgtattt tgtgaatatt ttgatattgg
      481 gcgagtatct gaagtatatg gaatttcttc cgcaactttc gaattctctt gattatatac
      541 tactttctct ttaattactt gagaatctga aattgaaaaa tccggcaaac tataaacatc
      601 tgtaatactt gttgttggat cagaatctct aacctgagaa accggacgat aatttaaatc
      661 cttacataca aagtcaggat tattaactat ctttgtagtt atatcttcgc ggtcataatc
      721 attaatttct gctcttaatt cctcaaacat tttttctttt tttatagcac aaactttaaa
      781 attacaaaat ttatacatgc cataacaacc atttggatca gtcatttctg ctacagaagg
      841 catagttgta tcgcaaactt taaacaaaga ataatccgaa aaatctgcca tgtttgtaaa
      901 ttggatacaa tatttcacag atttgtaacc ttcgctaatt gaatcagcaa atattggggt
      961 tgcagaaaat aacaacccac aaaatatcaa aaaacttaaa attatttttt tcatcatttt
     1021 ttccccattt tattttttta aatatttaag aatacttgag agttcgaaag gatctctaaa
     1081 gcaaagcacc tttcttttct caaactcttc tatctttttt tctgtcttat ttccaccaat
     1141 tgttactacc attattggtt tttttgtgtt tgcttttagc tttgctattt cttctggtat
     1201 cttgtttgag atgcttggca gttgtagcaa cattaaaaca attatggagt ttgtgttttt
     1261 gtcttttgca cacagataaa ttgcctgtaa gaacctttta tcatctgcat ctcctgtcaa
     1321 gtctatggga ttaccgattg ttgcataact cggaattatc ttttttattt tgtctgttgt
     1381 ttcttttgtt atttctgaaa gctttagatt attaatgtca agctggtcag ttgtaagaac
     1441 tccaaagccg ccgccgtttg ttataatttg tgagtcttta atattatcaa cactatacca
     1501 agaaaagaac ctagaaatat caaacatttc ttttatggtc tttacttcta ttgctccggc
     1561 ttgtcttaaa gctgttgaaa aaacttttat tgaaccagca attgctgctg tgtggctttt
     1621 aacagctgtt gttcctttct ctgagattcc gccttttaga actattactt ttttcttttg
     1681 cgttactttt tttaaagttt catagaattt ttttccatct tttactcctt caatataaca
     1741 caaaattatg tcagtatctt tgtcttttcc aaaatattct aataaatcta tttcatcaat
     1801 atctagagca ttaccataag acacaaactt gtttatctct agtctttgct tgcttgccaa
     1861 atctagaaga gcaataccta aagcaccaga ttgcgtaatg attgaaactt ttcctgactt
     1921 tggtaaatca tatttatctc tagaattaaa ggtcgcgtct agatgattag aaagattaag
     1981 aagacctaag cagtttgggc caataagatt tattttttct tttaatatta tttttttaat
     2041 cttctctgta aggtctgttc ttccaatctc tgaaaaacca gaagttacca ttagtatgtt
     2101 tttacatttg gattttgcta tatctttaac aacttcaaaa gttattgctg ctggtgttgc
     2161 aacaacagaa agatcaatct tctctgaaat ctctgtgatg cttgaaaaga cttttttatc
     2221 tagtagcttc tttcctgaaa gcctggggtt tgtaaaatat actttgcctt taaagcccga
     2281 gaggttctta gcaattgtgt tgccaacact tccctttttg tctgaagcac caataagaca
     2341 cacgcttttt ggttcaaaaa aattatttaa gtctttcaaa attatcacta ttttatttat
     2401 tctatattac tatatattaa aaatatatat ataagggttt ctttcttatg ttttaataca
     2461 aaatggtaag tgaaaacaca tgaaagacaa ttttgaaata atgatatatg gcagagcagg
     2521 gcttggtgca aagacttgtg cacaactgat tgcagattct gcacttcttg atggaaaagt
     2581 tgttcaggca tttccggagt atggcccaga aagaagaggc gcgccagtta ggtcatttgt
     2641 aagaatttca aataaacaga ttagagtaca tgaaccaatc actcacccag atttagttat
     2701 tgttattgat tgcgggttgt ttaatgttct accagggctt tctaattttg attgtgatta
     2761 tataatcaat agcgacaatg acaaggcgtt ttttaaaaaa tttattaaaa atgagaaaaa
     2821 gataaatgtt attgatggca gtaagatttc tatggaatta ttaaaaataa acaatccaaa
     2881 tgctgttttg attggagcat ttgttaattt atttggagtt attaaatatt cttctgtaga
     2941 agaatctata aaaaaagaat ttgaaaaaaa agggaaagca gatattatta aaccaaatct
     3001 tttatgtcta aagaaaggat ttgagcattt taagtgatga ttatggctga aactaaaaag
     3061 ataggaggcg ttcttcctgg aggaacagct caagtaaata agactggctc ttggagaagt
     3121 ttaagaccag aatgggactc aaagaaatgc atacactgta tgctttgctt taatgcttgt
     3181 cctgaacagt gtataaatgc aaataaagac gctaaaggga atattaaaag gcaagaaaca
     3241 aatcttgatt attgtaaggg ctgcgggata tgcgcagaaa tctgtccagt taaatgtata
     3301 aaaatggtaa aagaatgaat tcatttacag aagacattga aaagattgaa acaatcataa
     3361 aggtaaaaga aagtacaagg tttggaatag tcggagatag gtttgaaagc tctgctgaac
     3421 acacatttag ctgtattctt cttgcgcagt atttcattaa gaagattcct tacaagttag
     3481 atgaactaaa agtaattaaa ttactaagct atcacgatat tcaaaatgtt ttttttaaag
     3541 acataaatct ttcagacgat tcttggagta ataaaaaaga tttagaaaaa gttgcattta
     3601 agaaaacaat caatagtctt ccttcagcaa ttgcaggaga ctatgaaaaa aattatttag
     3661 aatttgaagg gttgctaaca acagaaagta gatttgctaa agctattgat gttttagaac
     3721 cagtaataca tgcttataat caaaaaaaac attggaaaga aggaaaatgg acagaaagta
     3781 aattaagaat gcacaaagaa aaatatttag atgaatttcc agtattaatg aaattcttta
     3841 atgattttgt agattatgca aatcaaaatg attattttta taaggaataa agtgaaaaaa
     3901 tatgaaaaca acaaaattag gtttaagtgg cggagaagca attgcttatg cattaaaaca
     3961 aatcaatcca gaagtttttg caatgtaccc aattactcca caaactccaa tcattgaaag
     4021 ctattctaag tattcagcta atggcttagt agatactgaa attattagaa cagaatctga
     4081 acattctgct ctttctgcaa ccatcggtgc atcagcttcc ggagttcggg cagtaacagc
     4141 aactgcttca caaggccttc tttatatgtt tgaagcactt agtgttgcta gtggtttacg
     4201 ccttccaata gttatgcctg tggctaatag agcaatttct gcacctatta acattcattg
     4261 tgatcattca gactccatgt ctgctcttga tcaaggctgg atgcaagtgt attgtgagag
     4321 tgccgaagag gcttacgata tgacgctatt tagtttaaga cttgctgaat ctgtttctct
     4381 tccaatcatg gtttgtattg acggatttta cacaagtcac aatatggaaa tgattaatgt
     4441 ttatgatgat ttatctgtaa aaaaatttat aggagaattt aaaccaaact ataatctttt
     4501 agacacagat aaaccaataa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 ntatgaagaa tactctaaag ccttaacaaa agcaaaaaaa ataattgttc ttgaaagaac
     4681 agaaggggca ggaagctttc ctcctcttta caaggatatt tgtttaactg taatgaatac
     4741 cacaaataaa ccagaaataa tttctctggt ctttggccta ggcggaagag atatttatca
     4801 aaaagatatt gaagaacttt tagtttctgt aatctcagga aagaaactaa aaagcagata
     4861 tattggaatt aaggaataaa atatgaactc aaaagaactt gcaattaaat tattagaaaa
     4921 gaaagcaatg gtttctggtt acagaacttg tgctggttgc cctattgcaa tcatatgtaa
     4981 aactgtaatt gctgcaagcg acaaaccagt tgttgcatca agcgcaacat cttgtgctga
     5041 agtaacaaca acagtttatc cacaaactgc ttggaatatt ccttggatac attctctttt
     5101 tgcaaactct gcagcaacaa tttcaggagt tgaatgtgca tacaaggctc agaaaaagaa
     5161 aggcaaaata aaaaaagaca ttaatttttt agtaattgct ggagatggtg gaagctttga
     5221 tattggactc caatcacttt ctggagcact agaaagagga catgattttg tttatctttg
     5281 ttatgataat gagggatata tgaattgctt aagtaaggat tcattaattt acacagagtc
     5341 tggacttaaa aaaataactg aaattaaagt tggagaaatg gtttatgcat ttgatcaaaa
     5401 gacacacaag ttagtgctta aaaaatgcag tggagtattt gataatggaa ttaaaaaagt
     5461 ttatgaaatt gaaacatttc atcataacat caaggcaact ggaaatcatc ctttcttagt
     5521 ggcaaataaa ctttcaagag gaaaacctaa tactttgatt tggaaaacac ttgaggaatt
     5581 aaaaaaagga gatcaagtaa ttacattaaa aaaattaaaa ggaaataaac catttacttt
     5641 tgaaaaaata acactttcaa agaaaggcga ttataaagta aattctataa atgaagttaa
     5701 aatccctgaa aaatcaacaa ctgatttaat gttattctta ggactttatg ttggagatgg
     5761 ttggattaga gataaaggag aaattggttt tgctttacct gaaggaactg aaggaagaga
     5821 aaaatgcatt gctacttgta aaaaagtctt tgggttaaaa acaatcacta ctgataagac
     5881 ttacatctat atgaattcaa ttaatattac aaaattcatt aactcgcttg gatttaacaa
     5941 aggtgcaaaa aataaaataa ttccatcttg ggtttataca attcctgatg aacaaaaaga
     6001 agcatttatt gaagggttgt tattatctga tggatatatc cataaaaaga agaaagatag
     6061 ttgtagatat ctttctgcaa gtaaagaatt aattctaaga ctaagattat tattacaaac
     6121 actaaattac agggctggaa aaataactgt acaaaaaaaa gaaaaaggta cttttgtagt
     6181 ttatcgtcca ttattgaaag acgcaatata ttatagtgtt tgctttagta aaaaatatcc
     6241 gtggaataca actaaatatc ctactcaata taagtatcaa aactttttga ttggaaacga
     6301 aaactttgat actgaaatta taaaaaacat aaaagaaata ggtaaagaac aaacctttga
     6361 tttaagagtt gaaggagaac acaattttat tgcagacgga atcgttgttc acaatactgg
     6421 taaccaaaga agctcagcaa cgccaaaagg tgcagtgact tcaacaactc cttcaggaaa
     6481 gataagtttt gggaaattag agttttcaaa agatttaaca aagattgctg cagcacataa
     6541 tatcccttat gttgcacaga ttaattctca taatcaaatt gatatgtttg agaaagcaaa
     6601 aaaagctttt gaggttaatg gccctgctgt gcttgtgtct tttgcagcat gcccaacaaa
     6661 catgaaagca gattctaaat atactattaa gattagtaaa cttgctacag aaacttgctt
     6721 ttggcctctt tatgaagtga ttaatggcaa agtgataatt aattacaagc caacaaacaa
     6781 actgccaatt gaagaattcc taaaaacaca aactaaattt aaagatgttc taaaaccaga
     6841 gaacaaaaaa gtattacaag agatacaagc agaagtagac aaaagatggg cagagctatt
     6901 gaaactagag aaattataaa ttctctttag tctctattgc tatttctaat tcctcatttg
     6961 ttggaactac cattgctata actttgctgt cttttgttga aataatcgta tcgtgtttct
     7021 tgtttgcttc tttatctatt tttaaaccaa gatatgaaaa actatcaaga actaattctc
     7081 ttaagtatgc agacttttcc ccaataccac cagtaaagac aattacatct attccgttca
     7141 tctctgctat gtaactccca acaattgaaa caatccttgt tgtaagaata tcattagcaa
     7201 gttttgcgtt tttgtctcct gctattgaaa gatcttctat ttgtctgtga tcactactaa
     7261 tctggcttat tccaaataat cctgattctt tgttaatcat tttctttatt tgttctttgg
     7321 aatacccatt ctctaaaaga aaaagaataa tctctggatc aaagcttccg cttcttgtgc
     7381 ccattggaag accatcaact ggagtaaagc ccatagatgt atcaaaactc ttccctttat
     7441 tgattgcaca aatgctttgg ccattaccca aatgacaagt aattattttt aggttattgt
     7501 agtctttacc taacatctct gttgcttttt tagaaacata tttgtgactt attccgtgaa
     7561 agccatattt tctaatcttg aatttatcgt aaagttcttt tggaagacca taaagatata
     7621 tcttttctgg aattgtttga tgaaaagcag tatcaaagac tgcaaattgc ctagcttttg
     7681 ggagtttttc ttttagaatc tcaatgcatt caatattcac tggattatga agaggtgcta
     7741 aaaaagaaat ctttttaaga tgcaaaagca ttgcttctgt tatctctgtg gtctttgaat
     7801 actcttctgc accatgaact actctgtgcc ctataaatct tatgtcaaaa agattttcaa
     7861 ttatttttct ttcaataaga acagaaagca tatcttcaat tgcttgtctg tgtgttttta
     7921 tttctgtttc ttgtgtgctc tttgagccaa tggttaaaac tcttaaacaa gacttgccgt
     7981 ctgttctatt taaaccgtca tataatcctt ttattaatac ctctccatta tctggattaa
     8041 tgtattgaaa ctttagagag ctactgcctg catttattac tagtatgttc attataaaat
     8101 ctccttttta aaattagtat atatttatag aaacaaacta attataaaaa taagctttat
     8161 aaaatctttt gtctttgttt tattaatgaa ccaagagcaa acagaactta atttgaccaa
     8221 aaaaataatt ataggtatac aaatgctgtt tgtagcattt ggtgcaactg ttcttgtgcc
     8281 cttgcttgtt ggtattgatc ctgctgttgc tctttttact gctgggtgtg gaacactatt
     8341 attccatttt ataacaaaag gaaaagttcc agtattctta ggaagctcat ttgtttttat
     8401 tgcaccaata attaaagcca cagaacttta tggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnttgctct ttctagtgtt gttggaataa ctttaaatca agttcttcca
     8581 gataaagaaa aagatctaag aagcatttag gcttcttaaa ttaaatttaa tcaagttctg
     8641 cttctcttaa attactcttg ccttttaggc ctctaagaag ctggtctatt tctcttttgt
     8701 gttttctata ttcttctgca gttattcttt ttttcgcatt agaatctttt ttttgttcgt
     8761 gatttgtctt tttgtgagca tttctatttt ttcctagaac acttcctgtt gttgttaatt
     8821 ttcctcttgg catattattc ctccagttat ctaaaattta ataatattat ttataagaaa
     8881 atttcttttt caatcttttc aggcaaatac tttcttgcaa caaaatcaag agacttgtta
     8941 gcaagagcct gttgctctat tcttattttt ttatctatcg cggtctttaa ttctgattgc
     9001 cattctttgg atttaaacca atcatagttc attaattctt ttgctctttt aatgtctcct
     9061 tctttcatct tttcagtaac attttgtaag ccataagttt caacatcggt aattgtcata
     9121 ccaacatatc ttgcatcagg aactgccatg tatttagagt gggccgctaa tgccattgat
     9181 ccacttttta taacagaata aatataccaa ccataagggt ctccgtctgt aaaaacaatg
     9241 acaggaagat catactcgta tcttaatcta tgaactagtc ttcttgcgcc tcttgctcct
     9301 tgcccacctg taccgattaa tattgcgtta tgctgtctag catatctttc ttcaattaat
     9361 ctgttataca ttgcagcagt ttcaataact aaaacaaaat cagcattaac atcaactatc
     9421 tcaatctctt ctggttctat tctagacatt atgctccatc cgcctctacc aagagaatta
     9481 caattgaaaa tatcattatc gtgctttaga tctttaagag taatgtctcc ataaagagag
     9541 cctttcgctt ctgcatgaag ccctaatttt tctctaatgg tgtttgctgc gtgctctaca
     9601 tcaacaataa cttcatcaga ctcgccctgt ccttcaaagg tgttttcttt agtgtttgca
     9661 actgttcttt ttaattcata gtatatttct ctgattgacg cagttttccc ttcttttaaa
     9721 tattcattac atttgtcatt aacaagcatt gtctgcataa actttcttgc agctccaaga
     9781 tttaaaaaag gcctgactgt tttcttgtct ccaagctgaa tatatttttt atcttcatca
     9841 aaataaacat tactaaggcc tcttacttta gtagtaaagg ttgggtgtct gttgcctttt
     9901 atctgatcaa caatatcttt tgcaatctga agagttcttt gttcaatatc ttttgtggtt
     9961 tctgtttcat atttatctaa accttttttc tcgtgctttt taccagcttt tgtttctttt
    10021 tttgcggcca tatttattca cctattcttc aaactcctca tcttctacta cagagtcatc
    10081 gtcaaccact tcatcatctg aagtttttat tccttgttct tctaatctta gtttttcaag
    10141 aaccatatta tttagatttt ttaaaagtaa atctctattt ttttctcctg ttgtgtgtat
    10201 taatgcatca acaacatatg gagcgtactt tgcaaatacc tttttctttt cttttttttc
    10261 ttcttctctt ttcttttcaa taatatatat tcctactcta cgcgcaacat ccattaatgc
    10321 aagttttatt tcattaacaa tatctgagtc gtcagaaact gcagacttgc ctgcagaagt
    10381 gtatggtata tgaacagata ttagattaac aaaaatagtt attggacttg tatcaaaatt
    10441 actaactccg tatcttttcc aatcaacaga ttctactgtt tttgtgattg aacaaccgcc
    10501 tgcatcaaac agtaatggag ctttgtttgc atatcttata atttcagaag accttacctc
    10561 ttctattatt gtttgttttt tatctattgt tttttcaaca gaaattaatc ttccggattt
    10621 ccccccgtag gctactgctg cttcaacttg aaaagcatac cctccttgat agactgttgg
    10681 ttttctagta accacagaaa gaaactctgg ttcgactatt gactttaagg atttttttat
    10741 ttgttcttct ccaattggca caagattatc tgtgcttgga gctataaatt ttgtttgctt
    10801 tattgctttt attaggtctt cggcttcttc ccaagtcatt gctcttggat ccttatttaa
    10861 atcgaaatgc acaagttttt gaacattatc aacagaagtt tggccaaacc tatcaaaggt
    10921 tgtcatcaaa aaagaattta tctttctaga acctgcagaa tgtcttgcat aagttagcat
    10981 ttcgtcaaca gaaactccaa ttggatgtgg cttaactgct gttgggatat ttggaagttt
    11041 atttattgtt ctagtgtatt ttattttttc ccctaaaggg tcttgaaaac ttaaatttaa
    11101 atgaggatta gagatcgctg ttcttctaag atattcatca acacttgatt gtcctttttt
    11161 gtagattatg tctttaaatt tacatttaac tgcaaggccc ttaaatttat tttctaaata
    11221 atgtttttca gaaacttttg gagtgtttgt cttactatct attgacatct ccattgagat
    11281 tgcctttcct ttgtttgttc cagatattaa ttttgtgggt ttacctgttg tcatttgtga
    11341 taatagaata ataccactaa cacctattcc ttgctgacct cttgtttgaa ctaacctatg
    11401 aaacttagtt cctgcaagca atttacccat agcactagca agtttgtctt ctggtattcc
    11461 tgggccatta tctacaacag aaatctcata atattctgaa cctaattcag aaatagtcac
    11521 ctctatctct ggtagaatgc cagcttcttc gcaagcatct agagaattgg tcactaattc
    11581 gtgtattata gtagtcattg tcctttgctt accggaaagg cctagcatct gcttgttttt
    11641 cttaaagaag tcagctactg aatgctcttt aaactctgta aacagctctt ctgccccaac
    11701 ttcttttctt atagtttctt tagcttcttt ttctgccata aataatcaca taaaaatagt
    11761 tttaattttg gtctcaatag taatataata tatattatat ttataaatat attgtag
//
LOCUS       DAOOON010000020        11799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_6746_length_11799_cov_15.521458, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000020 DAOOON010000000
VERSION     DAOOON010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6746_length_11799_cov_15.521458"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLK55_03225"
     CDS             <1..371
                     /locus_tag="PLK55_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35968.1"
                     /translation="SEKLGVKDSIVIKIWLNGHFPKNIVPKPEVIADQIIKGRFKLPN
                     RINKITERTIIFENITHDVVIAALFQRLSGEKYNLRFKELPKELEALRIDISKEGKAI
                     MQFRDYKKEITDRLNAILTN"
     gene            458..658
                     /locus_tag="PLK55_03230"
     CDS             458..658
                     /locus_tag="PLK55_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOZ35969.1"
                     /translation="MAGKRDTISLIALILIIVGGLNWLLVGLFSFNLVTTVFGAIPIL
                     VTIVYTLVGLAAIWAIIKFKSI"
     gene            738..1028
                     /locus_tag="PLK55_03235"
     CDS             738..1028
                     /locus_tag="PLK55_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35970.1"
                     /translation="MRIIKKTTRKIKNKIKEKYKLKPNEKAILKNLEKIVDQRLKEYM
                     RKYSLYLSSNPRINPQPQLNMEAATKELNEAITRYNRYRKEYGLDPVNYKKK"
     gene            1045..2394
                     /locus_tag="PLK55_03240"
     CDS             1045..2394
                     /locus_tag="PLK55_03240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOZ35971.1"
                     /translation="MTNTNNNFEGLVKDKQILQAIHNIGIKTPTEIQEKVIPAILEGK
                     NIIAQSATGTGKTIAFLAGVLPSITKQKKVQLLIIVPTRELANQVYEETKKIAKVKEL
                     QACAIFGGASVGNQARQLKYADIVIGTPGRIIDQFERRNLKVNNIKYLILDEADRMCD
                     MGFSEDISRIIEQLPKERQTLLFSATITNDVSNIERKYISHAQRIVVQTYVDPKNLLQ
                     DYYLVKQNQKFSLLLHLVKQHSKKAIVFCNTRSEVDVIYNNLKEQRVECFKLHGGLEQ
                     NKRSGTIEKYHANADGLLISTDVSARGIHIDNLEYIYNYDLPKDNTQYIHRIGRTARA
                     GKKGRAISIITQKEEEDFLRMCKKFGFIVEAKVLPSIVPITLKRTKEEERALERINRY
                     QERREHFSKDRKDRKKENQKKMVIGELTEEDIKKKRMDFFKHKKKIRQKKYTNRGRR"
     gene            2394..2951
                     /locus_tag="PLK55_03245"
     CDS             2394..2951
                     /locus_tag="PLK55_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35972.1"
                     /translation="MNKKVKSKKLENVVYNIIMFTLIFALVISFVFTIDKLFLERPKD
                     NCYLQDYNYKPEVITNIDANCSYNQEYINSCYKSEGNIVYKKDCQMECSYCYRDYNKI
                     LETYNNRINIGRMIFTFLLALLMAFILIKDKIIRYSILSASLVSLFVATISATKLLNK
                     MLPVVIIAEFLLVIFIYRKTSKEKK"
     gene            complement(2959..3702)
                     /locus_tag="PLK55_03250"
     CDS             complement(2959..3702)
                     /locus_tag="PLK55_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ35973.1"
                     /translation="MKTPIMPTINYKKFSWNELDKIDQNTITQISEIDNLFYRSSSDL
                     EEYLRKIKINADKTIIYLCYRGKELVGAVFGTLNRVSEFCNKDYKIKAKPKDYMFLLT
                     EFFVKPGFQNNGIGTALMARVFADQRAKYDSKFIYMKAFRKTQTINKKLTGITPFIVP
                     IKEVAEIKRIVNNLSIIEKRKKRNISHNKSMKTGALKRKIFRNFKNSKYKYDLIETSN
                     PSITDVKDHYIVINKNPKYKRKQIRSIAK"
     gene            3769..4104
                     /locus_tag="PLK55_03255"
     CDS             3769..4104
                     /locus_tag="PLK55_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HOZ35974.1"
                     /translation="MKKILKMTKKITKIKLTEEELKRMKNKLKRQQTQFKKEQEQMRK
                     EVNEKLITLITGAFSLVAALAWNETIKTIFQRVFGTAQTVWAMIIYAVLVTIIAVWIT
                     YWMTKRNKN"
     gene            4188..4412
                     /locus_tag="PLK55_03260"
     CDS             4188..4412
                     /locus_tag="PLK55_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35975.1"
                     /translation="MFPGYWSEGAGDKTCSKCLRFRPNVTNKNKGTCVGRDVVATGGC
                     EKFAKKKTTKVATIKKKTTNIIKTKKKAKK"
     gene            4409..4651
                     /locus_tag="PLK55_03265"
     CDS             4409..4651
                     /locus_tag="PLK55_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35976.1"
                     /translation="MTLNLAIIGLAIIFIAWLFQFFSMNKRREISTVFIIAYIIGTIL
                     LVIDGYKVGLIANTIINSIIAIAAICVLIRFSKPKK"
     gene            4663..5340
                     /locus_tag="PLK55_03270"
     CDS             4663..5340
                     /locus_tag="PLK55_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOZ35977.1"
                     /translation="MADKNGNGVTGDIPIATVRSELLAIIRKKNQKATEDGFITREEL
                     KIARQEYIESILKKEKGELNKIDKQVVKSLVEHESIVKDIMQEKENLTVGQKLADGVA
                     KFGGSWGFIISFGVFLIIWIGINSYFLLQKPYDPYPFILLNLILSCLAALQAPIIMMS
                     QNRKDQKDRIRAENDYKINLKSELEIRHLHEKMDNLVQKQWQRLLEIQRLQLDIMEET
                     QKERKKR"
     gene            5354..5596
                     /locus_tag="PLK55_03275"
     CDS             5354..5596
                     /locus_tag="PLK55_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35978.1"
                     /translation="MVKKKRQIKKVKTEKNIKYEIYWDYFSLVMFLVFSTLFISMLII
                     EIFFVLGKSALWLTIPVTAILWALSYLLARILFKKK"
     gene            5683..6903
                     /locus_tag="PLK55_03280"
     CDS             5683..6903
                     /locus_tag="PLK55_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOZ35979.1"
                     /translation="MFSNRPLFDYITKKEFTKIKKFAKGKKTPFLIIDLQKIEEKYDE
                     LIKNMPFAKIYYAVKANPEDDIIKLLARKGSNFDVATNYELDQLLRLKISPKKISFGN
                     TIKKEEDIAYAYKKGIRLFATDSISDIKKLAHAAPGSKVFFRIIMEGGSADWPLSRKF
                     GAHPDMIYHLIFEAKKLGLHPYGVSFHVGSQQRDIGQWDQAIAQCKYLFDAVKQEGIE
                     LKLINMGGGFPANYISPTHDLETYSKEITRFLEEDFGEHFSKKLEIIIEPGRSIAADA
                     GVIVTEVVMISKKSLSNQFDWVYLDVGKFGGLIETLDESIKYPIFTENYRKPPKEKDC
                     REVILAGPTCDSTDTLYEKHKYLLPKSLKEGDRLYILSTGAYTRSYSSIYFNGFPPLE
                     IYIMKKGSKKKKEE"
     gene            6905..7525
                     /locus_tag="PLK55_03285"
     CDS             6905..7525
                     /locus_tag="PLK55_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35980.1"
                     /translation="MKKKIQTKISKYEEKKKTNFFSKNNRVKIIIWVLAILCVLLIIT
                     FSLKYLNNNNAETNTQETMPELGINEETENPNLETTPVIISGEITKSSKLEDFYATSK
                     PSFIVFAGTYCGHCQKLLPELEKEIWNNYSNKANIWVEIIGDGEFPVAMPQGVNSNII
                     YEEIMGDCRYVPAYVVLDKEGNQILRSCGSEKTIAEIKAALDSQLN"
     gene            complement(7657..7878)
                     /locus_tag="PLK55_03290"
     CDS             complement(7657..7878)
                     /locus_tag="PLK55_03290"
                     /inference="COORDINATES: protein motif:HMM:NF021415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HOZ35981.1"
                     /translation="MSQKCIICGRAIGNYGIYCPKCQEDLEKKIQKREKFEEVYRSAD
                     FFERMKLKETKDALKARQNRLAQKPVSKK"
     gene            complement(7949..9442)
                     /gene="lysS"
                     /locus_tag="PLK55_03295"
     CDS             complement(7949..9442)
                     /gene="lysS"
                     /locus_tag="PLK55_03295"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOZ35982.1"
                     /translation="MGRLEEYQENRKNNFSSIVNMGIEPYPYTYDRTHVSKDIKEKYK
                     DLEPDKYTTEIASVAGRVLSLRSLGKISFIDLHDTYGKMQIVVKNETTSPENLELLKK
                     LDDGDIIGVKGKIFKTKRGELSIDAQDITFLSKAFMPLPEKFHGIQDAELKYRQRYLD
                     LMTSKESRDIFFIRAKIIKIIREILDKKGFLEVETPLLQSLYGGANARPFITESFVWK
                     RQLYLSISPELYLKRLLVGGFDKVYTICKNFRNEGIDRSHNPEFTMLECYEAFKDYNS
                     AMDLIEEIYETIALELNGSTRVTYLEQTFDFKRPWKRLTMAGALKEYANLDVDKLSDS
                     DLANLLKENRLELETYKRGLAISELFEHFCENHLLGPVHIIDHPKETTPLCKLHRSNK
                     DLIERDEPYVNYSEAGNIYSELNDPMLQEKLMLDQADQGRGKGENHPVDTDFIESLKY
                     GMPPAYGLGLGIDRLVMIFTNSANIREVILFPMMKEEGDGLEDNEKK"
     gene            complement(9495..>11799)
                     /locus_tag="PLK55_03300"
     CDS             complement(9495..>11799)
                     /locus_tag="PLK55_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35983.1"
                     /translation="DAKENTEDADAKENTEDADAKENTEDADAKENTEDKGLLDFITK
                     NKNTPKLKSQVDYKSISVDTVTSDGIKTNNYLLENTSEKNLKVGQTLISFSHHTDYDG
                     TMAGQSTEKAIDLIESREKEETCGQKVTNLTFLGAPIGSVIPKSFRNHRFVVGSMIAQ
                     KFAYLVLPGSGYFNSFIGPAIVSDIPMQTMIMPEIHGCIDDEEGLYAHFFVSAQEEER
                     QNKDSKNKVGEAIIDGTKKVEEVVSKVTSGTELEKGVKAGSDKVKEYVEENIIEDPIV
                     QATFTTGGGTNTAVKGQLFFIEIGPKTKCMASGYNDKGVEVLKDKDTNIDVTIDKEKG
                     ILTVTDENGTRTVIGEGSKDFVRLIGDNLGIPAKVIPRSLTYIPLPNNTEPLFEIDAH
                     GNLTVKNSEFFDCLRAGYEAQTGLTLNSTSKNLTPYLGIVKLTNDINPIGAYTSSFLG
                     NSIISQGAGLPRLIANGNTSKLTVLGNRSTTIGPIDGKENRTGKNISVQFEFGQLVYS
                     EEAKAYIMWVETTTETSGNDIASMKAEVTKTKAINGCENEEIGINFSTSPVKDNDQAA
                     ANTEKLNKALEKVGPFQMFDTKTKTFIFYVSDPPECEQRLKIIDKETGEIYDAKISSF
                     EQTPNGISVKTDDGQTHNLEFSAEDGVPRLTYNGEKDTLTSAQGKNGSFWYDPETGNW
                     YTTNGNLIPLNDAFRDGVSFGVDGNGKVTGTPGTNVFNIGSAGSGKGSGGFNIPLTPE
                     STVMIILYITVILSGFVLIYMDRKKRK"
BASE COUNT         4370 a         1764 c         1749 g         3916 t
ORIGIN      
        1 tttctgaaaa attaggagta aaagattcaa ttgttattaa aatttggtta aatggtcact
       61 tccctaaaaa tatagtacct aaaccagaag taatagcgga ccaaataata aaaggcaggt
      121 ttaaacttcc aaacaggata aataaaataa ctgaaagaac aattattttt gaaaatataa
      181 cacacgatgt tgttattgct gccctatttc aaagactttc tggagaaaaa tacaatctaa
      241 gatttaaaga actgcctaaa gaactagagg cattaagaat agatatatct aaagaaggaa
      301 aagcaataat gcaatttaga gattataaaa aagaaataac agacagatta aatgcaatat
      361 taactaatta atttgaatca agcagtctat ttattagggt atttaaacat ttcttattat
      421 aaatattaat ataacatata acacggaggt tataaaaatg gcaggaaaga gagacacaat
      481 aagtttaatt gcactcatct taataattgt aggcggttta aactggttac ttgtaggact
      541 ttttagtttt aatcttgtaa ctacagtatt cggtgcaata cctatattag taacaatagt
      601 ttatacactt gtaggacttg cagcaatttg ggctataata aagttcaaaa gcatataaat
      661 tatgcttttt ctttttttat tttaatctta atttataaac tatttgtttc ttattatttt
      721 aaataaagtg aggaactatg cgtattataa aaaaaactac cagaaaaata aaaaataaaa
      781 taaaagaaaa atataaactt aaaccaaacg aaaaagcgat attaaaaaat ctggaaaaaa
      841 tagttgatca aagattaaaa gaatatatga ggaaatactc tttatattta tctagtaatc
      901 cacgaattaa tccacagcca caactaaata tggaggctgc gacaaaagaa cttaatgaag
      961 caattacaag atataataga tacagaaaag aatatggact tgaccccgta aattataaaa
     1021 aaaaataaat gggaaaaata aaacatgaca aatacaaata ataactttga aggccttgtc
     1081 aaagataagc aaatactaca ggcaatacat aatattggca taaaaacgcc aacagaaata
     1141 caagaaaagg ttattcctgc aatacttgaa ggcaaaaaca taattgctca atcagcaaca
     1201 ggtacaggaa aaactattgc ttttcttgca ggtgttttac catcaattac aaaacaaaaa
     1261 aaagttcaat tactaattat agtacctaca agagaacttg ctaatcaagt ttatgaagaa
     1321 accaaaaaaa tagcaaaagt taaagaactt caagcctgtg ctatatttgg cggcgcttct
     1381 gttggtaatc aggcaagaca attaaaatat gcagacattg ttataggaac gcccggaaga
     1441 ataattgacc agtttgaaag aagaaatcta aaagttaata atattaaata tttaatatta
     1501 gacgaagcag acagaatgtg tgacatgggt ttttcagaag atatctcaag aattattgag
     1561 caactaccaa aagaaagaca aacgcttctt ttttctgcaa ctataacaaa cgatgtctca
     1621 aatatcgaaa gaaaatatat ctctcatgca caaagaattg ttgtgcagac ttacgttgac
     1681 ccaaaaaatc ttttacaaga ctattatcta gtcaaacaaa atcagaagtt ttctttgctt
     1741 ttacatcttg ttaagcaaca cagtaaaaaa gctattgttt tttgtaatac tagaagcgag
     1801 gtagacgtta tttataataa tctaaaagaa caaagagtag aatgctttaa attacatggc
     1861 ggcctagaac aaaataaaag atcaggaacc attgaaaaat atcacgcaaa tgcagacggg
     1921 ttattaatat ctacagatgt ttctgcaaga ggtatacaca ttgataatct agaatatatc
     1981 tacaattacg acttgccaaa agacaataca caatatatac atagaattgg aagaacagca
     2041 agagccggaa agaaaggaag agcaatatca atcattactc aaaaagaaga agaagacttt
     2101 ctaagaatgt gtaagaaatt tggttttata gttgaagcaa aggtcttgcc gtcaattgtt
     2161 cctataactc taaaaagaac aaaagaagaa gaaagagcac ttgaaagaat aaacagatat
     2221 caagaaagaa gagaacattt cagtaaggat agaaaggata gaaaaaaaga aaaccaaaaa
     2281 aagatggtaa ttggagaact aacagaagaa gatattaaaa agaaaagaat ggattttttt
     2341 aaacataaga aaaaaataag acaaaaaaaa tacaccaata gaggtagaag ataatgaata
     2401 aaaaagtaaa atcaaaaaaa ctagaaaatg tagtatataa tattataatg tttactttga
     2461 tttttgcttt agttattagt tttgttttta caatagataa attattttta gaaagaccaa
     2521 aagataattg ttatttacag gattataatt acaaaccaga agttattaca aatatagatg
     2581 ctaattgttc ctataatcaa gaatacatta attcttgtta taagtcagaa gggaacattg
     2641 tttataagaa agactgccaa atggaatgta gctattgtta tagagattat aacaagatat
     2701 tagaaacata taataataga ataaatattg gcagaatgat ttttactttt cttttggctt
     2761 tgttaatggc ctttatatta attaaagata aaattataag atactctata ctttcagcat
     2821 cgcttgtatc tttatttgtt gcaacaatct ctgcaacgaa gttattgaat aagatgctac
     2881 cagtagtaat tattgcagaa tttttacttg taatatttat atatagaaaa acaagcaaag
     2941 aaaagaaata gaataaaatt attttgcaat acttcttatt tgctttcttt tatattttgg
     3001 atttttattt attacgatgt aatgatcttt aacatcagta attgatggat tagaggtctc
     3061 tattaaatca tatttatatt ttgaattttt aaaattacga aatatcttcc gttttaaggc
     3121 gccagttttc attgatttat tatgtgatat gtttcttttt ttacgttttt caattattga
     3181 aagattattt actattcttt taatttctgc cacctctttt ataggcacaa taaatggcgt
     3241 tattccggtt agttttttat ttatagtttg ggtttttcga aatgctttca tatatataaa
     3301 tttagaatca tatttggctc tttgatctgc aaatactcta gccattagtg ctgtaccgat
     3361 gccattattt tgaaatccag gcttaacaaa aaactcggtt aataaaaaca tatagtcttt
     3421 aggttttgct ttaattttat aatctttatt gcaaaattca gaaacacgat ttaaagtacc
     3481 aaaaactgca cccaccagtt ctttccccct ataacacaaa taaattattg ttttatctgc
     3541 atttattttt attttacgta gatattcttc taaatctgaa gaggatcgat aaaataaatt
     3601 gtcaatttct gaaatttgtg ttatagtatt ttgatctatt ttatctaatt cattccatga
     3661 aaatttttta taattaattg taggcattat tggtgttttc atataataaa taataccgca
     3721 gaaagtattt taaaacaata gactataatt ttttatattg gtgattatat gaaaaaaata
     3781 ctaaagatga caaaaaagat tactaaaatt aaattaacag aagaagaact taagagaatg
     3841 aagaacaaac taaaaagaca acaaactcaa tttaagaaag aacaagaaca aatgagaaaa
     3901 gaagttaatg aaaaattgat aacattgatt actggggcat ttagtcttgt cgcagctctt
     3961 gcctggaatg aaaccattaa aaccatattc caaagagtat ttggaacagc tcagacagtc
     4021 tgggcaatga ttatttatgc ggttttagtt actataattg cagtttggat aacctattgg
     4081 atgacaaaac gaaataaaaa ttaggagaaa tcctaacaaa agatatataa atataatata
     4141 tctatatata aatagaaaaa aatacaattt taaaaaggag caaaaaaatg tttccaggat
     4201 attggagtga gggcgcagga gataaaacct gttcaaaatg tttaagattt agaccaaatg
     4261 ttacaaacaa aaataaaggc acttgcgttg gtagagatgt tgttgcaaca ggtggctgtg
     4321 agaaatttgc aaaaaagaaa actacaaaag tagctacaat taaaaaaaaa acaactaata
     4381 taattaaaac taaaaagaag gcaaagaaat gacattaaat cttgcgatta ttggtttagc
     4441 aataatattt attgcttggt tatttcaatt cttttctatg aataaaagaa gagaaatatc
     4501 tactgtgttt attattgcat acattattgg aacaatctta ttagtaatag acggttacaa
     4561 agtagggctc atagctaaca ctattataaa tagcataatt gctattgcgg caatctgcgt
     4621 attaataaga ttttctaaac caaaaaaata atttgaatat atatggctga taaaaatgga
     4681 aatggtgtaa ctggagacat acctatcgcc acagtcagat cagaactttt agcaattatt
     4741 agaaaaaaaa accagaaagc aacagaagac ggatttatca ctagagaaga attaaaaatt
     4801 gcaagacaag aatacattga aagcattctt aagaaagaaa aaggagaact aaataaaatt
     4861 gataaacagg ttgttaaaag ccttgtagaa cacgaatcca tagttaaaga cataatgcaa
     4921 gaaaaagaaa atctaacagt gggtcaaaaa cttgcagacg gtgttgcaaa gtttgggggt
     4981 tcttgggggt ttattatttc ctttggtgtt tttcttatta tttggatagg aataaatagt
     5041 tatttcttat tacaaaaacc atatgaccca tatccattta tactattaaa tttaatattg
     5101 tcttgtttag ctgcattaca agcaccaata attatgatga gtcaaaatag aaaagaccaa
     5161 aaggatagaa taagagcaga aaacgattat aagataaact taaaatcaga attagaaatt
     5221 agacatttac acgaaaaaat ggataatctt gttcaaaaac aatggcaaag attactagaa
     5281 atacaaagat tacaattaga tataatggaa gaaacacaaa aagaaagaaa aaagagatag
     5341 gaggaataat aaaatggtta aaaaaaagag acaaataaaa aaggttaaaa cagaaaagaa
     5401 tataaaatat gaaatatact gggattattt ttcattagtt atgtttttag tattttcaac
     5461 attatttata tctatgctta ttattgaaat attttttgtt ttaggtaagt ctgcattatg
     5521 gctaactatt ccagttactg caatactttg ggcgcttagc tatttattag caagaatatt
     5581 atttaaaaag aaataattga aattaacaaa aaattcattt tttatttttt ttattttata
     5641 ttgtaaaata tgactaaata aaaaggagat taaagaacaa atatgttttc aaataggcca
     5701 ctctttgatt atataacaaa gaaagagttt acaaaaataa aaaaatttgc aaaaggcaaa
     5761 aaaacaccat tcttaattat agatcttcag aagattgaag aaaaatacga tgaattaatt
     5821 aagaatatgc cgtttgcaaa aatatactat gctgttaaag caaatccaga agatgacatc
     5881 attaaattgc ttgcaagaaa aggttctaac tttgatgttg caacaaatta cgagcttgat
     5941 caattattaa gattaaagat atcgccaaaa aaaatatctt ttggtaacac aattaaaaaa
     6001 gaagaagata ttgcttatgc ctacaagaaa ggcattagac tatttgcaac agattcaatt
     6061 agtgatataa aaaaacttgc acatgcggct cctggctcta aagttttctt tagaataatt
     6121 atggagggtg gttctgctga ttggccacta tctagaaaat ttggtgcaca cccagatatg
     6181 atttatcatc ttatttttga agcaaagaaa ttaggattac atccctatgg cgtatccttc
     6241 catgttggtt cacaacaaag agatataggc caatgggatc aagcaattgc acaatgtaaa
     6301 tatttgtttg atgccgttaa acaagaaggg atagaattaa agctaataaa tatgggcggg
     6361 ggatttccag caaactatat aagcccaaca catgacttag aaacatattc taaagaaata
     6421 actagatttt tagaagaaga ctttggagaa catttctcta agaaattaga gatcataatt
     6481 gagcctggtc gttcaattgc agcagatgca ggagttatag ttacagaagt tgtaatgatt
     6541 tctaaaaagt ctttatctaa tcagtttgac tgggtatatc ttgatgttgg taaatttgga
     6601 ggactaattg aaacacttga tgaatcaatc aaatatccta tctttacaga aaactataga
     6661 aagccaccaa aggaaaaaga ctgcagagaa gtaatacttg caggacctac ctgtgattct
     6721 acagacacac tttatgaaaa gcataagtat cttttgccaa agagtctaaa agaaggagat
     6781 agattataca tcctttctac aggcgcatac actagaagtt atagttctat atactttaat
     6841 ggttttccac ctcttgaaat ttatattatg aaaaaaggat caaaaaagaa aaaagaagaa
     6901 taatatgaaa aagaaaatac aaacaaaaat tagtaaatat gaagaaaaga aaaaaactaa
     6961 ttttttttct aaaaataata gagttaagat aatcatctgg gtattagcaa tactttgcgt
     7021 gcttttaata ataacttttt ctttaaaata tttgaataat aataacgcag aaacaaatac
     7081 tcaagaaaca atgccagaac ttggaattaa tgaagaaaca gaaaatccaa atttagaaac
     7141 tacgccagta ataatctctg gagaaataac aaaaagctca aagcttgaag acttctatgc
     7201 tacaagtaaa ccaagcttta ttgtgtttgc aggaacctat tgtggacatt gtcagaagct
     7261 tttacctgaa ctagaaaaag aaatctggaa taattattca aacaaagcaa atatatgggt
     7321 agaaataatt ggagatggag agtttccagt agctatgcca caaggagtta attctaatat
     7381 catatatgaa gaaataatgg gagattgccg ttatgttcca gcttatgttg ttctagataa
     7441 agaaggaaat caaatattaa gatcctgtgg ttctgagaaa acaattgcag aaataaaagc
     7501 tgcacttgac agccaattaa attaattcac aaattattgg acaaaaaagt tcaaaaaccc
     7561 aaaaaaatta ataaaatttt atggcccaga aaaattaaaa ttttttgaga actagataat
     7621 ttttcttggg aaaaaaccgt cctcggtttt tccattttac tttttagaaa caggtttttg
     7681 agctaatcta ttctgtctag cctttagtgc atctttagtt tcttttaatt tcattctctc
     7741 aaagaaatca gcacttctgt agacttcttc aaacttttct cttttttgta ttttcttttc
     7801 aagatcttct tgacattttg ggcaatatat gccataatta ccaattgctc ttccacaaat
     7861 aatacatttt tgactcattt ttttctatcc ccttttaaat tttaattaaa tattgtaata
     7921 ataagtatat ctaatatatt taaatacctt actttttttc gttatcttct aagccatccc
     7981 cttcttcttt catcattgga aataatatta cttctcttat gtttgcggag tttgtaaata
     8041 tcatgacaag gcggtcaata ccaaggccaa gaccataagc tggtggcatt ccgtacttaa
     8101 gggattcaat aaagtcagtg tctactggat ggttctcgcc ttttcctctt ccctggtctg
     8161 cctggtcaag cattagcttt tcttgtaaca ttgggtcgtt taactcgctg taaatgtttc
     8221 ctgcctctga gtaattaaca tatggctcgt ctctttctat caagtcctta ttgcttctat
     8281 gaagcttaca aagaggagta gtctcttttg gatggtcaat aatatggact ggcccaagaa
     8341 gatggttctc acagaaatgc tcaaacaact cagagatggc taggcctctc ttataggtct
     8401 ctaattccaa tctgttttct ttaagcagat ttgctaagtc gctatcgctt aatttatcaa
     8461 catcaagatt tgcatactct ttaagagcgc cagccatggt taatctcttc catggtctct
     8521 tgaaatcaaa ggtttgctca aggtaagtaa cccttgtgct gccgttaagt tctaaagcta
     8581 tggtttcata aatttcctca atcaaatcca ttgctgaatt ataatcctta aaagcctcgt
     8641 aacattcaag cattgtaaac tctggattat gagatctatc tatcccttcg tttctaaagt
     8701 tcttacaaat agtatatacc ttgtcaaaac cacctacaag tagccttttc aagtaaagtt
     8761 caggagaaat tgaaagataa agctgtctct tccatacaaa actctcagtt ataaatggtc
     8821 tggcatttgc tccaccataa agggactgaa gcaaaggtgt ctctacttct aagaaaccct
     8881 tcttgtcaag tatttctcta ataatcttaa taatctttgc tctaataaaa aatatatccc
     8941 ttgattcttt agaagtcatt aagtctagat atctttgtct atatttcagc tcagcatcct
     9001 gaataccatg gaatttctca ggaagaggca taaaggcttt tgataagaaa gttatatcct
     9061 gtgcatcaat tgaaagctct cctctttttg tcttaaagat ctttccttta acaccaatta
     9121 tatcaccatc atcaagcttt tttaaaagct caagattttc aggacttgta gtctcgttct
     9181 taacaactat ttgcatctta ccataagtat catgtaaatc aataaacgaa atcttaccaa
     9241 gagatcttaa agaaagaact cttccagcaa cactagcaat ctctgttgta tatttatctg
     9301 gctctagatc tttatacttt tccttaatat ccttagaaac atgcgttcta tcataagtat
     9361 aaggataagg ctctattccc atatttacta tagaactaaa attatttttt ctgttttcct
     9421 gatattcttc taatcttccc atttttaatc acatctaaat atatatttaa gaaaaaaaat
     9481 aaatataaat ggatttattt tctttttttc ctgtccatat agattaaaac aaatccactt
     9541 aatattactg ttatgtaaag aattatcata actgttgatt caggagtcaa aggaatatta
     9601 aagcctccgc ttcctttccc tgaccctgca gaaccaatat taaacacatt agttcctgga
     9661 gttccagtaa ctttaccatt accatcaaca ccaaaggaaa caccatctct aaatgcatca
     9721 tttagtggta ttagatttcc atttgtagta taccaattac ctgtctctgg gtcataccag
     9781 aaagaaccgt tctttccttg agcagaagtt aaagtgtctt tttctccatt ataggtaagc
     9841 cttggaacac cgtcttctgc actaaactca agattatgtg tttggccgtc atcagttttt
     9901 acagaaatac catttggtgt ttgctcaaaa gaagatatct ttgcatcata gatttctcca
     9961 gtttctttat ctattatctt tagtctttgc tcacattctg gtggatcact tacatagaaa
    10021 ataaaagttt tggtcttagt atcaaacatt tggaaaggcc caactttttc taaagcctta
    10081 tttagttttt ctgtgtttgc tgctgcttga tcattatctt ttactggaga tgtagaaaaa
    10141 ttaataccaa tctcttcatt ttcacaacca tttattgctt tggtctttgt gacttctgct
    10201 ttcatgcttg caatatcatt accacttgtt tctgttgttg tttctaccca cataatatag
    10261 gcttttgctt cttcactata aacaagctga ccaaactcaa actgaacgct tatgttttta
    10321 ccagttctat tttcttttcc atctattggg ccaattgttg tagatctatt tcctaaaaca
    10381 gttaatttag aagtattgcc attagcaatt aatcttggaa gaccagcgcc ctgagaaata
    10441 attgaattcc ctaaaaaact agaagtatat gccccaatag gattaatatc atttgttaat
    10501 tttacaattc caagatatgg agttaagttc ttacttgtag aattcagggt aaggccagtt
    10561 tgtgcttcat aacctgccct tagacaatca aaaaactctg aattttttac agtaagattt
    10621 ccatgagcat caatttcaaa taatggttct gtattgtttg gtaatggtat ataagttaaa
    10681 ctacgaggta tgacttttgc aggaatacct aagttatctc caattagtct tacaaaatct
    10741 ttgctacctt caccaataac cgttctagtg ccattttcat cagtaactgt taatattcct
    10801 ttttctttat caattgtaac atctatattt gtatctttat cttttaatac ttccacacct
    10861 ttatcattat agccacttgc catacatttt gtttttggac caatctctat aaagaaaagt
    10921 tgtcctttta ctgctgtgtt tgttcctcca ccagtagtaa atgttgcttg aacaattgga
    10981 tcttcgataa tattttcttc aacatattct tttactttat ctgaaccagc ttttactcct
    11041 ttttcaagtt ctgttccaga agttactttt gaaacaactt cttcgacttt ttttgttcca
    11101 tcaattattg cttctcctac tttattttta gaatctttat tttgtctttc ttcttcttga
    11161 gcagaaacaa agaaatgagc ataaagtcct tcttcatcat caatacaacc atgtatttct
    11221 ggcataatca tagtttgcat agggatatcg cttacaattg cagggcctat aaatgaatta
    11281 aaataaccag aacctggcaa aaccaaatat gcaaattttt gtgctatcat agatccaaca
    11341 acaaatctgt gatttctaaa ggatttggga atgacgctac ctattggcgc ccctaaaaaa
    11401 gttaaatttg taaccttttg cccgcaagtt tcttctttct ctcttgactc aattaaatct
    11461 attgcttttt ctgtagattg accagccatt gtaccatcat agtctgtatg atgagagaaa
    11521 gaaataaggg tttgtccaac ttttaaattt ttttcagaag tattctctaa taaataatta
    11581 tttgttttta taccatcaga agtaacagta tcaacactta ttgatttata atcaacttgg
    11641 ctttttagtt ttggcgtgtt tttgtttttt gttataaagt ctaatagtcc tttgtcttct
    11701 gtattttctt tagcatctgc atcttctgta ttttctttag catctgcatc ttctgtattt
    11761 tctttggcat ctgcatcttc tgtattttct ttggcatct
//
LOCUS       DAOOON010000021        11361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_7130_length_11361_cov_19.773129, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000021 DAOOON010000000
VERSION     DAOOON010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7130_length_11361_cov_19.773129"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1379
                     /locus_tag="PLK55_03305"
     CDS             <1..>1379
                     /locus_tag="PLK55_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35984.1"
                     /translation="DDENGYYIDFVTTDSNDGATIIIDDINYPLVDDSNNPYTPIHLL
                     LDGGSWSFKRIVSDGDTIIDFEPDDSSYSIIIPSDINSEQSIDLNLSGIIYGDDEELL
                     VPDGEDFYLERQTATTKYSVTIPAGTTITGEDGWDGLITLPIITDNFTITNGAADVTI
                     NIGSGFGLTFDKAVKILIGDMNGKSAAYNDGTTHLITACNTDLSDANSETATTDLNSW
                     NKTDCSSSNGTDLIIWTKHFTDFAAYTPTEEDDDGGPGGGSDDDEEEDEDEASCSDID
                     ITTTSITIYSDSTSTKSIALINDSEDFDFEIEDITITEATGIDVSIVDEPEDVASEDS
                     EYLKIELETDEFSSATTKTIELEIEGVFDDDDETDCSIDQDISVRIQLVSADEEGEEP
                     EVVEADTNATTYNGNTSDDGAIDGTTDDSGANAISDKTEQSVTPTKKKSNDWLFWLLD
                     LSIVVVIIA"
     assembly_gap    1380..1479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1480..1746
                     /locus_tag="PLK55_03310"
     CDS             <1480..1746
                     /locus_tag="PLK55_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35985.1"
                     /translation="HWTATPEKNVPFLIKILLVIKMSDINQIAVFEEKNIRRIWHQED
                     WWYSVIDVVGALTESSEPKRYWSDIKIKLKDESGIEPYDYIVPL"
     gene            1908..2153
                     /locus_tag="PLK55_03315"
     CDS             1908..2153
                     /locus_tag="PLK55_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35986.1"
                     /translation="MGTITISLDKKSENKLRDCATIMYGTRKDALSKTIVTAIDKLEE
                     DNVKKRDILTECLDNPIKVKGKMPKTRAELYDRKIFD"
     gene            2131..2547
                     /locus_tag="PLK55_03320"
     CDS             2131..2547
                     /locus_tag="PLK55_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ35987.1"
                     /translation="MTERFLIDTNILVYAFTDLDIKKKEKARETITFAQSSGQGYISK
                     QNIIELINTTHKLKIREPNSQLCKVISGLEFLRIIDYSTGSIKKALDLETKANLDFFD
                     SLIVQTMLENDIYTIYTENEKHFNRIENLKVINPFK"
     gene            complement(2550..4856)
                     /locus_tag="PLK55_03325"
     CDS             complement(2550..4856)
                     /locus_tag="PLK55_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOZ35988.1"
                     /translation="MKIGNYTVGDYIVDNEGGKKKLIFDCRHCVYNTSISDDPRCRYH
                     VMKILETVDADEVILAEVYERVYTQKQTSFLKDMVDLLLDFENRAVWVSSHLKVDDLD
                     CDDCYPQRHNNIIRFARDLLTYDPIASYISLVKELENTKARAEEQAGRDCEKCFKANL
                     KHLLSLKEQYDKTEFIKILKPILTKVKDYNDLTDVYKTVFEAQIKPAFIGSQLLRSDA
                     EKFELIDEYQVLTTTVQIFKHPEKIEFIYNINPPEYTLSPDHYFVLSKTKDTVANYQP
                     GKVTLTSGTGTRKYFQRIYQSTIADVARKYGIALAPDDITALSEIVTRYTIGYGILEM
                     VLSDRNITDVYIDSPIGGRPIYIVHSKYGQCQTNILYTDKEAESLVGKMRAMSGRPFD
                     DAHPILDFDLPDLNTRVAAIGRPLATDGIAFAFRLHKDTPWTLPQLINVKSLSPLAAG
                     MLSFFIDNSSTILVAGSRGSGKTSLMTALIQEILQNKRIIVQEDTLELPIMYFKEIGF
                     NAQRLKTKSAIGSDEESAEVKPEDALRTALRLGDSAIIVGEVRSVEAKVLYEAMRIGA
                     AGNVVMGTIHADSAYAVWDRVVNDLGVPTTSFKATDLVIVTKPIRFKGSLKSYRRVVQ
                     ITEVKKHWNTDPDAEGGLIDLMTYDAGKDALILNEDALKDSDLFTKISTLSGMSIEEI
                     WDNIKLLANEKEYQVELARELKCMELLEAEYTIAVHNKLLLIKEEWIEEHGKIDFNAV
                     LIEWKKWLKEDFAPKIVKRINENKEKEE"
     gene            complement(4926..5393)
                     /locus_tag="PLK55_03330"
     CDS             complement(4926..5393)
                     /locus_tag="PLK55_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35989.1"
                     /translation="MDMLMMAIKNMNALIIVISAFVFIFIFIYFARSGFGKGKSKIKL
                     YKTEYNYGETISGKLIVNAKKQFVAESIDVSIVLERKKGDLTSFRKDITLEKNITFNV
                     GDNKEFEFKIDLPSFDSPKISGRNLNSKVWKIKSQVKISNFVVLYDSKNIKVR"
     gene            5535..6854
                     /locus_tag="PLK55_03335"
     CDS             5535..6854
                     /locus_tag="PLK55_03335"
                     /inference="COORDINATES: protein motif:HMM:NF020230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35990.1"
                     /translation="MQLTSEQIYSIIKTQSGLSDEELNQKLEEIKEKYQGLLSDVGAN
                     ILLSKQLGVNLDLKQKTSAVTKISEINPSIDSVTLYARIKSIPPVKTYKSKDGGHGKI
                     QAVYLEDETGIIKLNLWQDKAEIIKEQGFEKNDLVLIKDAAINQYNEKVELSLRQGGQ
                     VIKDPEGVDIKNIKENVIKISGIDAAPEGTVDTYARIIAIYPIKTFKKEEKERFVINF
                     EISDGLKSIRCIAFDAWAQEISNNFKKGDLVRLCDVKIKDGLYDLELYVNWNSTLTKD
                     PKTKIKIPPLSELVSNDVKEEKINALQNNSSYKIIGTIISVNKGNLRSFKCPDCKEKV
                     FIINNEFICEACNKPVEPDMNIFSSVTLDDSTGVIKVSLFNQLLESVYDIKKEDLKKD
                     LSQEEKDAIFSKAESKILGKKAIVTGKAKQNGFSNQLEFMASTFEIE"
     gene            6858..7808
                     /locus_tag="PLK55_03340"
     CDS             6858..7808
                     /locus_tag="PLK55_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35991.1"
                     /translation="MKTEFKYIPPFLPQEILQKIKIVEKNNIRTITFHNKEQLEFAKK
                     SKDFLEDFLDFYGSSRIDKSRIKFGGKEFRPNVFFDTVLLEYLSNCAYTEKKEIINSL
                     VKLSGKYSEIELANKFSLFLINLKKLSKSYAKDGLHKSIMPTLRSLYNNTIPREPHVI
                     KFNKKCKETYSGFIRAIRSGTITTDKVVVDGKERRMFFKSLVGSTETDVFTKRENKSL
                     KSKEIDLNEGHLELALDPMTFDIYAIRTKDRIHFGTPAEKLLGNWMPKDKEQFMKELV
                     KTELEKHLNKVYAKRKNGKVIVPGIQRMKETHRRMSSKAR"
     gene            7931..10057
                     /locus_tag="PLK55_03345"
     CDS             7931..10057
                     /locus_tag="PLK55_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HOZ35992.1"
                     /translation="MTEFDFDEVDEGLSGQTNLALDEYISVFTDFLEAVYKKEIEILA
                     SNYPDKKSLDIDYKKLEAYDLNIAERLIENPNLIIEAATAALRRIDVGILEQTDQKFE
                     PHIRFFNLPEEYRVDIRDIGSKHLTSLISAEGMIRQITERLQKMTDAHFICRKCQNSY
                     TIKQTTQTLTKPIMCDCKSREFDLDLEQSKFIDYQKLEIQEPIENLKGSEQANHVEVF
                     ISDDLVNSCSAGDKIIVTGIVKLKPQKDTNMFNKYMIANHVEKVDQEFEELDITPDEI
                     EEIKQLSKKEDIYELLAQSIAPKIYGHDIVKQAITLQMFGGVKKKVQKQDFRGNIHIL
                     LVGDPSTGKSELLRNAATIAPKSIFVSGKSASGAGLTVSAVKDDFGEGGWTLKAGAVV
                     LASGGIAMIDEFDKMETEDRSTLHEAMAQATVSVSKAGLYAKFRADTSILAAANPKFN
                     RFDKFKNPIEQIDLPFSLLSRFDLYFIIRDVLNRTLDLELGRHIIDVQEAAQKLSAPE
                     GELTKEEMEKIQDTVLPKIDSNIFKKYVAYARQQIRPKLSKEASEKILNYYIDLRDLG
                     RKSESFGATPRQLEGLIRLSEASAKVRLKHTIELEDAQRAINIFRISLEQTAIDMETG
                     KIDIDIITTGTSTSERQFIKRVLNIIKDMTLDGKAVTIAEISETMEKQNIGRDKLMDA
                     ISKLKKSGELYEPKVGLIKPVEQQPM"
     gene            10065..10913
                     /locus_tag="PLK55_03350"
     CDS             10065..10913
                     /locus_tag="PLK55_03350"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF018279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease"
                     /protein_id="HOZ35993.1"
                     /translation="MYSALKDRKFLLSFLFLFLLVQIIAIIVTINYLPQNLQLTLVNK
                     DPNSVWNALFIVGYIIFFTAIIIVLRKIFKKQNYLVVIEALALFGGVSLIFSIFLHTI
                     LAYIATICLLLIKYSFTKETIYTKWYNNILLAMAIAGAGAIIGISLGIIPVIVLLILL
                     AVYDIISVFFTKHMITLADMIIKKKISLLFILPTKKREYKLGGGDLVIPALVSSSLFA
                     LLIKKYSILQILIPISAIWISSIAGLFLTFYILDRYKTKVKALPALPIQVALMLVVII
                     ITLISS"
     gene            complement(10899..11300)
                     /locus_tag="PLK55_03355"
     CDS             complement(10899..11300)
                     /locus_tag="PLK55_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ35994.1"
                     /translation="MAKKRKKLEKHLSLDLFKPGKITVYVYVIVICLIIGFAILSFVS
                     SLNKTDKYKTQAIDDCIALCNEKLIRTELSSTGPCLSESIAPGWVCDSVNTPRNDYVD
                     NLKENQCESYHSRINKRFVEVSTTCEFLRTN"
BASE COUNT         4074 a         1750 c         1854 g         3583 t
ORIGIN      
        1 atgatgatga aaatggctat tatattgatt ttgtaacaac agattctaat gacggcgcaa
       61 caataataat tgacgatatt aattatccat tagttgatga ttctaacaac ccatacaccc
      121 caattcattt gttattagat ggaggaagct ggtcctttaa aagaatagtt tcagatggag
      181 atacaataat tgactttgaa ccagacgatt caagctatag tataattatt ccttcagata
      241 tcaattcaga acaatctatt gatttaaatc tttctggaat aatttatgga gatgatgaag
      301 aattgttagt tccagatggc gaagatttct atcttgaaag acaaacagca acaacaaaat
      361 attctgtaac aatacctgca ggcacaacca taacaggcga agacggttgg gatggtttaa
      421 ttactcttcc aataataact gataatttta caatcacaaa tggcgctgca gatgttacaa
      481 tcaatatcgg tagtggtttt ggtttaacct ttgataaagc agtaaagatt ttaattggag
      541 atatgaatgg caaatctgca gcatataatg acggtacaac acatctaata actgcctgta
      601 atacagactt aagtgatgca aattcagaaa ccgcaacaac cgatctaaac tcttggaata
      661 aaacagattg ttcttcttcg aatggaacag atttaataat ttggacaaag cactttacag
      721 actttgcagc atacacccca acagaagaag atgacgatgg cggcccaggt ggaggctctg
      781 atgatgatga ggaagaagac gaagatgaag caagttgttc agatatagat ataacaacca
      841 cgtcaataac aatttattca gatagcacaa gcacaaaatc aatagctcta ataaacgatt
      901 ctgaggattt tgattttgaa atagaagata taacaataac agaagcaaca ggtatagatg
      961 tttctattgt cgatgagcca gaagatgttg cttctgaaga ttcagaatat ttaaaaatag
     1021 aattagaaac agacgaattt agttctgcaa ccacaaaaac tattgaatta gaaatagagg
     1081 gcgtctttga tgatgacgat gaaactgatt gttcaataga tcaagatata agcgtaagaa
     1141 tacagcttgt ttctgcagat gaagaaggtg aagaaccaga agtcgtggag gcagatacaa
     1201 atgcaacaac atataacggc aatacaagtg atgacggagc tatagacgga acaactgacg
     1261 attctggagc aaatgcaatt tcagacaaaa cagaacaatc agtaactcca acaaaaaaga
     1321 aatcaaatga ttggcttttc tggcttttag atctttcaat tgtagtagta attattgcan
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc actggacagc tacccccgaa
     1501 aagaacgtcc cctttcttat caagatatta ttggtaatca agatgagcga tataaatcaa
     1561 atagcagttt ttgaagaaaa gaatataagg agaatatggc accaagagga ttggtggtac
     1621 tctgtgattg atgtagttgg agcattgact gaaagctctg agccaaagcg atattggtca
     1681 gatattaaaa tcaagctgaa agacgagtct ggaattgaac cgtacgatta tatcgtaccg
     1741 ttgtaattaa ttgctgaaga cgggaaatta agagaaacgg attgtgctaa tcttgaaggt
     1801 atctttagaa taatacaatc aattccttca aagaaagcag agcccttcta aacaaatggg
     1861 tttttaaatt gttttattaa ttaattattt aattaaatag tgatattatg ggaacaataa
     1921 ctatatcttt ggataaaaaa tcagaaaata aattaagaga ttgtgcaaca ataatgtatg
     1981 gtacaagaaa agatgcacta agtaaaacaa tagttactgc aatagataaa ttagaagaag
     2041 acaatgtaaa aaaaagagac attttaacag aatgtttaga taaccctata aaagtaaaag
     2101 gcaaaatgcc taaaacaaga gctgagcttt atgacagaaa gatttttgat tgatacaaat
     2161 attttagtct atgcatttac tgatttagat attaaaaaaa aagagaaagc gcgagaaaca
     2221 attactttcg cccaaagctc tggtcaagga tacattagca aacaaaatat aatcgaatta
     2281 attaatacca ctcacaaatt aaaaataaga gagccaaata gtcaattgtg taaagtaata
     2341 tctggattag aatttttaag aataatagat tattctacag gatctataaa aaaagcacta
     2401 gatttagaaa caaaagccaa tttagatttt tttgattctt taatagttca aactatgcta
     2461 gaaaatgata tatatactat ttacactgaa aatgaaaaac attttaatag aatagaaaac
     2521 ttaaaagtaa ttaatccatt taagtaaccc tattcttctt tttctttatt ttcatttatt
     2581 ctttttacaa tctttggagc aaagtcttct tttaaccatt tcttccattc tataagaaca
     2641 gcattaaaat caatcttgcc atgctcttct atccattctt ctttaatcag aagtagctta
     2701 ttatgcacag caattgtgta ttctgcttca agtagttcca tacatttaag ttctcttgca
     2761 agttctacct ggtattcttt ttcattagct aaaagtttaa tattgtccca gatctcttca
     2821 atagacattc ctgataacgt tgaaatcttt gtgaaaagat cagagtcctt aagtgcgtct
     2881 tcgtttaata ttagtgcgtc ttttcctgca tcataagtca ttagatcaat tagtccgcct
     2941 tcggcatcag ggtcagtatt ccagtgtttt ttaacttctg taatctgcac aactcttcta
     3001 taacttttta atgaaccttt aaatctaatt ggttttgtca caataacaag atctgttgcc
     3061 ttaaatgaag ttgttggaac accaaggtca ttaacaactc tatcccaaac agcatatgca
     3121 gagtctgcgt gtattgttcc cataacaaca ttacctgcag caccaattct catagcttca
     3181 tataagactt tagcttctac acttctaact tctccaacaa taattgcaga gtctccaagt
     3241 cttaaagcag ttcttaaagc atcctctggc ttaacttctg cagattcttc gtctgaacca
     3301 attgctgatt ttgtttttag tctttgtgca ttaaatccaa tttctttaaa gtacattatt
     3361 ggcaattcaa gtgtgtcttc ctgaacaatt attcttttgt tctgtaatat ctcctgaatt
     3421 aacgcagtca ttaacgaagt tttaccagaa ccacgagagc cagcaactaa gattgttgag
     3481 ctgttatcaa taaagaacga taacattcct gctgctaaag gacttaaaga cttaacattt
     3541 atcagttgtg gtaaagtcca tggcgtgtct ttatgtaatc taaatgcaaa agcgatgccg
     3601 tctgttgcta ggggcctgcc aattgctgca actctagtat taagatcagg aagatcaaag
     3661 tctaagattg ggtgagcatc atcaaaaggc cttccagaca ttgctctcat ttttccaact
     3721 aagctttctg cttctttgtc agtataaagt atatttgttt ggcattgtcc atatttgcta
     3781 tgcacaatat agattggtct acccccaatt ggtgaatcaa tataaacatc tgttatgttt
     3841 ctatcagaca ataccatttc taatatgcca taaccaatag tatatctcgt tacgatttca
     3901 gaaagtgcag tgatatcgtc aggcgccaaa gcaattccat attttctagc aacatcagca
     3961 atcgtgcttt gatatattct ttgaaagtat tttctagtgc ctgttccaga agttaaagtt
     4021 actttccctg gctgatagtt agcaaccgtg tctttagtct ttgacaaaac aaaataatgg
     4081 tctggcgaga gagtatactc tggcggattg atattataaa taaattcaat cttttcaggg
     4141 tgtttaaata tttgaacggt tgtagttaat acttgatact catcaatcaa ttcaaatttc
     4201 tctgcatcgc ttcttagaag ttgagatcca ataaatgcag gcttaatctg tgcttcaaaa
     4261 acggttttat aaacatcagt tagatcgtta tagtctttta cttttgttag tattggtttt
     4321 aaaattttaa taaattctgt tttatcatat tgttctttta aagataacaa atgtttaaga
     4381 ttagccttaa agcatttttc acaatctctt cctgcttgtt cttctgctct ggcttttgtg
     4441 ttttctaatt ctttaacaag agagatatat gacgcaatcg ggtcatatgt taaaagatct
     4501 ctagcaaacc taatgatatt attatgtctt tgcggataac agtcatcaca atccaagtcg
     4561 tctactttaa gatgagagga cacccagact gctctattct caaaatccag taataggtct
     4621 accatgtctt taagaaaaga agtttgtttt tgagtatata ctctttcata gacttctgct
     4681 aagattactt catcagcgtc tacagtttct aatattttca tgacgtgata tctacatcta
     4741 gggtcatcag aaatagaggt attatacaca caatgtctac agtcaaagat aagctttttc
     4801 tttcccccct cattatctac tatgtagtct ccaaccgtgt agttaccaat cttcattttt
     4861 tattcttgtt ttaaatatat tctagtaaat atatatcata ctttatgttt atatatgtta
     4921 ttaatttatc ttacttttat atttttacta tcatatagaa ctacaaagtt agatatttta
     4981 acctggcttt ttattttcca aacttttgag tttaaatttc taccagatat tttaggtgaa
     5041 tcaaaacttg gcaaatcaat tttaaattca aattctttgt tgtcgcccac attaaaggtt
     5101 atgttctttt ctaaagttat gtcttttcta aatgatgtta ggtccccttt ctttctttca
     5161 agaacaatgc tcacatctat actttctgca acgaattgtt ttttggcatt tacaattaat
     5221 ttccctgaaa tagtttctcc ataattatac tctgttttat atagtttaat tttagatttg
     5281 cctttaccaa aaccacttct tgcaaagtaa ataaaaataa aaataaaaac aaatgcagaa
     5341 atcactataa ttaacgcatt catatttttt atagccatca ttaacatatc catatctcta
     5401 atccccacat tttaattaga aagattaata acaaaatgtt ttaaaaataa ttgtttttct
     5461 tttttcgcac tggacaagtg gtctaggtaa ctttttaata tattggtttc ctaaatatat
     5521 attaggtgaa aagtatgcaa cttacaagtg aacaaatcta ttctattatt aaaacccaaa
     5581 gtggattaag cgacgaggag cttaaccaaa agcttgaaga gataaaagag aaataccaag
     5641 gccttttaag tgatgttggc gcaaacattc ttctctctaa gcaactagga gttaatctag
     5701 acttaaagca aaagactagc gctgtaacta aaatttcgga gattaatcca agcatagatt
     5761 ctgttactct ttatgcaaga ataaaatcca ttcctccagt caagacctat aaatcaaaag
     5821 acgggggcca cggaaagatt caagcagttt atttagaaga tgaaactgga ataattaaat
     5881 taaatctttg gcaagacaaa gcagagatta ttaaagagca aggctttgaa aagaacgatt
     5941 tagttttgat aaaggatgca gcaattaacc aatacaacga aaaagtagaa ttgtctctaa
     6001 gacaaggcgg ccaagtaatt aaagaccccg aaggcgtaga tattaaaaat attaaagaaa
     6061 atgttattaa gatttcagga attgatgctg cgcctgaagg cacagttgac acctatgcaa
     6121 gaattatagc aatttatcca ataaagactt ttaaaaaaga agaaaaggaa agatttgtta
     6181 ttaattttga gataagtgac ggtcttaagt caataaggtg tattgctttt gatgcttggg
     6241 cacaagagat ttcaaataat tttaaaaaag gcgatcttgt aaggttatgt gatgtaaaaa
     6301 tcaaagatgg gttatatgat ttagagttat atgttaattg gaattcaaca ttaacaaaag
     6361 atccaaaaac aaaaattaag attccaccat taagcgagct tgtttcaaat gatgttaaag
     6421 aggaaaagat taatgctctt caaaacaaca gtagctacaa gattattgga acaatcatct
     6481 ctgttaacaa aggaaattta aggtcattta aatgcccaga ctgtaaagaa aaggtcttta
     6541 taatcaataa cgagtttatt tgcgaggctt gtaataaacc agtagagcca gacatgaata
     6601 tcttttcttc agtgacgcta gatgattcta caggcgtaat aaaagtaagt cttttcaatc
     6661 agcttttaga atcagtttat gatattaaaa aagaagactt aaaaaaagac ttaagccagg
     6721 aagaaaaaga cgcaatattc tctaaagccg aaagtaagat tcttggaaag aaagcaatag
     6781 ttacaggaaa agcaaagcag aacggttttt ccaatcaact agagtttatg gcaagcactt
     6841 ttgaaataga ataaaacatg aaaacagaat ttaaatacat tccgccgttc ttaccacaag
     6901 agattttaca aaaaataaag attgttgaaa aaaataacat aagaacaatt acttttcata
     6961 ataaagaaca actagagttt gcaaagaaat caaaagattt cttagaggat ttcttggatt
     7021 tctacgggtc ttctagaatc gataaatcca gaataaagtt tggaggaaaa gagtttaggc
     7081 caaatgtttt ttttgataca gtactattag aatatttaag caattgcgca tacactgaaa
     7141 aaaaagaaat tatcaattca ttagttaagc tttctggcaa gtattcagaa atagagctag
     7201 caaacaagtt ttctttgttt ttaataaatc taaaaaaact aagtaaatct tatgcaaaag
     7261 acggcttaca taaatccata atgcctacat taagatcttt atataataat acaataccaa
     7321 gagaaccaca cgtaataaag tttaataaga aatgtaaaga aacctattct gggtttatta
     7381 gagcaataag gtctggcact ataactacag acaaggtagt tgttgatggt aaagaaagaa
     7441 gaatgttttt taagtctctt gtaggttcaa cagaaacaga tgtttttact aaacgcgaaa
     7501 ataaatccct aaagtcaaaa gaaatagacc taaatgaagg ccatctagaa ttggctttag
     7561 atcctatgac ttttgatatt tatgcaataa gaacaaaaga cagaattcat tttggaacgc
     7621 cagcagaaaa actattgggt aattggatgc caaaagataa agagcagttt atgaaagagc
     7681 tagtaaaaac agaacttgaa aaacatttaa acaaagttta tgcaaaacga aaaaatggca
     7741 aagtgattgt tccaggaata caaagaatga aggaaacaca cagaagaatg tcttctaaag
     7801 ccagatagtt tggttttttc cccttcactt cctgtattgt ttttgttagt ttttatgaat
     7861 aactataggc ttttcctaag tagacaagca tataacgcat ttgacataat ataatatagg
     7921 ggattaatat atgacagagt ttgattttga tgaagtcgat gagggcctaa gtggtcaaac
     7981 gaatttagct ttagatgaat atatctctgt gtttactgat tttctagaag cagtctataa
     8041 aaaagaaata gaaattctag cttcaaatta tccagataaa aaaagcctag atattgatta
     8101 taaaaaacta gaagcctatg atcttaatat tgcagaaaga ttaatagaaa accctaattt
     8161 aattattgaa gcagcaactg cagctctaag aagaattgat gttggtatct tagagcaaac
     8221 agaccaaaag tttgagccac atataagatt ttttaatctc ccagaggaat atcgtgttga
     8281 tattagagac attggttcaa agcatcttac aagtttgata tcagcagaag gaatgattag
     8341 gcaaattaca gaaagactac aaaagatgac agatgctcat tttatttgta ggaagtgtca
     8401 aaattcttat actattaaac aaacaactca aactctaaca aagccaatca tgtgcgactg
     8461 taagtctaga gagtttgatt tagatcttga acaaagtaaa tttattgatt atcaaaagct
     8521 tgagattcaa gagcctatag aaaatctaaa aggtagcgaa caagcaaacc acgttgaggt
     8581 ttttatttct gacgatcttg ttaattcttg ttctgctggt gacaagataa ttgtaactgg
     8641 tatagttaaa ctaaagccac agaaagacac aaatatgttt aacaagtaca tgatagcaaa
     8701 ccatgtagaa aaagttgatc aagaatttga agagcttgat ataactcctg atgaaattga
     8761 agaaattaaa cagctttcta aaaaagaaga catctacgag ttattagcgc aatcaattgc
     8821 tccaaagatt tatggtcacg acattgttaa gcaagcaatt actcttcaga tgtttggtgg
     8881 tgtaaaaaag aaagttcaaa aacaggattt tagaggaaac attcacattc ttctagtcgg
     8941 tgatccatct accggtaagt ctgaactttt aagaaacgca gcaaccatcg ctccaaagtc
     9001 tatttttgtt tctggaaaaa gtgcatcagg tgccggatta acagtctctg cagttaaaga
     9061 tgattttggc gaaggcggct ggactctaaa ggctggtgca gtagttctag cttctggcgg
     9121 tatagcaatg attgatgaat ttgataagat ggaaacagaa gatagaagca ctcttcacga
     9181 agccatggct caagctacag tttctgtttc taaagcagga ctttatgcaa agtttagagc
     9241 agacacttct attctagcag cagcaaaccc aaagtttaat agatttgata agtttaagaa
     9301 tccaatagag caaattgatc tgccattttc tcttctttct agatttgatt tatattttat
     9361 aattagagac gttttaaaca gaactcttga cctagagctt ggaagacaca taatcgatgt
     9421 tcaagaggct gctcaaaagc tttcagcacc agagggagaa ctaacaaaag aagaaatgga
     9481 aaagatacaa gatactgttc ttccaaagat agattcaaat atttttaaga aatatgtagc
     9541 atatgctcgt cagcagatta gaccaaaatt aagtaaagaa gcttctgaaa agattctaaa
     9601 ttattatatt gacttaagag atcttggcag aaaatccgaa agctttggtg caaccccaag
     9661 acagcttgaa ggtttaataa gattatcaga ggcaagcgca aaggtgcgat taaaacacac
     9721 aatagagctt gaagatgcac aaagagcaat aaatatcttc agaatttctc ttgagcaaac
     9781 tgcaatcgat atggaaactg gtaagattga tattgatatt ataacaaccg gcacctcaac
     9841 aagcgaaagg caatttataa aaagggttct aaacataatt aaagatatga ctcttgatgg
     9901 aaaagcagta actattgcag aaatatcaga aactatggaa aaacaaaata taggaagaga
     9961 taaattaatg gatgcaatat caaaattaaa gaaatcagga gagctttatg aaccgaaagt
    10021 tggtttgata aagccagtag agcaacaacc aatgtgatta aaaaatgtat agtgctttaa
    10081 aggatagaaa gtttcttctt tcgtttttgt ttttgttttt attagttcaa ataattgcaa
    10141 taattgtaac aatcaattat ctgccgcaga atctacaatt aactcttgtt aataaagacc
    10201 caaatagtgt ttggaatgct ttatttattg ttggctatat cattttcttt accgcgataa
    10261 tcatagtttt aagaaaaatt tttaaaaaac aaaattatct tgtagtaata gaggcgctag
    10321 cattgtttgg tggtgtttct ttaatttttt ctatattttt acatacaatt ctagcatata
    10381 ttgcaacaat ttgtttatta ttaataaaat attcttttac aaaagaaacc atttacacaa
    10441 aatggtataa caatattctt ttagctatgg caatagctgg cgctggcgca atcattggta
    10501 taagccttgg cattattcca gttattgttt tgttaatact tcttgcagtc tatgatatta
    10561 tctctgtgtt ttttacaaag cacatgatta ctcttgcaga catgattatt aagaaaaaga
    10621 tatccttatt atttattctt ccaacaaaga aaagagaata taagcttggc ggtggagatc
    10681 ttgttattcc ggcactagtc tcttctagtc tttttgcctt attaataaag aagtattcaa
    10741 tattacaaat attaattccc atttctgcaa tttggatttc ttcaattgct ggattgtttt
    10801 taaccttcta tattttagac cggtataaaa caaaagtaaa agctcttccg gcccttccaa
    10861 tacaggttgc gctaatgctg gttgtaataa tcattacatt aattagttct tagaaactca
    10921 caagtagtgc ttacctctac aaatcttttg tttattcttg aatgatagga ttcgcactga
    10981 ttttctttaa gattatccac ataatcgttt cttggagtat taacagaatc acaaacccac
    11041 cctggagcaa tagattcaga aaggcaaggg ccagtagaag acaattcggt tcttataagt
    11101 ttttcattac aaagagcaat acaatcatca attgcttgtg tcttatattt gtctgtttta
    11161 tttaagctag aaacaaaact aagtattgca aatccaataa taaggcaaat aacaattaca
    11221 taaacataga ccgtaatttt tcctggctta aacaaatcca aacttagatg cttttctaat
    11281 ttttttctct ttttagccat tttattaaat cacatataat tataataact aaatacttaa
    11341 aaagttattt attattaaat a
//
LOCUS       DAOOON010000022         9627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_8796_length_9627_cov_15.469808, whole genome shotgun sequence.
ACCESSION   DAOOON010000022 DAOOON010000000
VERSION     DAOOON010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8796_length_9627_cov_15.469808"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..820
                     /locus_tag="PLK55_03360"
     CDS             392..820
                     /locus_tag="PLK55_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HOZ35995.1"
                     /translation="MPGLKTDVGLEDDYSFDDIDIKAPKEEVEEEEKEEKVTFDILDD
                     DEEEETKGKGKRKKVKKRARKVKEEIKHSYIPKHEIVSGDELEKLISEKNINPEKLPL
                     IYIADPGIAHLEVKVGDVIRIARNNKKIGEVIYYRRVISI"
     gene            870..2336
                     /locus_tag="PLK55_03365"
     CDS             870..2336
                     /locus_tag="PLK55_03365"
                     /inference="COORDINATES: protein motif:HMM:NF007175.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HOZ35996.1"
                     /translation="MKSLDKWPVIESYYDLYSPVQQQINSFNQFIDEKVSEIVEENGF
                     ISSTNNVDVTLSNVEITNPQIVEADRTAKFRLMPMEARLRNLTYSGSIYLDIKLYRKG
                     VLQDEKRAYVGEMPILVKSNRCNLYGLSPEELIENGEDPMDRGGYMIVNGSEKALITQ
                     EVLAFDRILLNDTEEVINAQVISVKGAFKGKVRISRTQDGLFTISFPSSPRKLMLVEL
                     LNAIGYTKSTHILDLFPKDQIIQNEILLNLDKVELQSEEEALDKIGRSVAPSQTKTYR
                     LRRAKEVIDSFLLPHLGQEKDARTMKAHFLVAMSVKIIEKAYNLRTQADKDHYANKRL
                     ELSGKLMENLFRFSFKHFTKDLKFQIDRTISRHRKLNINTVIRPDAITERIMFAVSTG
                     NWIGQLTGVSKYMSRDNFLTSITDLRRVKSTLDSSRELYEARDVHGTHWGRLCPIETP
                     DGPNCGLVKNLSMMAKITTDTDPEGIRRILKNEDVTSD"
     gene            2361..4181
                     /gene="rpoB"
                     /locus_tag="PLK55_03370"
     CDS             2361..4181
                     /gene="rpoB"
                     /locus_tag="PLK55_03370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HOZ35997.1"
                     /translation="MAKSRAKIYVNGGIVGFHDDPEKLRADLLKKRRSLQIDPQINIA
                     FNETTNEVYINTDAGRVQRPVIILDKGQSRVTPEIVEDLRSKKLSWQDLLSKGIVEFL
                     DAEEEENAYIALTEDEITPDHTHLEISGTAIFSIVSSMIPFIEHNMAGKAIHGAKLYK
                     QAIGIPSSNYNLRFDTEMHCLYYPQKPLVYTRTSKLLHMEKRPMIQNAIVAIMPYKGF
                     NMLDAFIINQGAIQRAMSRSSYYRSYSQAEIRYPSGQTDKFCIPTEEASGFRGTDAYA
                     NLDENGIVMLESKISEGGVVIGMTSPPRFVEEVSEFGVVTEERRDTSVCSRKHLDGYV
                     DRVMISETISGTKLCKVRIRTYLEPQVGDKFTSHHAQKGVVGAVIREEDMPFTSQGIK
                     PDFILNPHSIPSRMTLGHLFESIAGKTVAASGKAIDGTPFCNDKDEVFKLLQEYGFRP
                     DGKETFYDGKTGEKIEMPIFVGPLGFERLQTHLVVNRIQARDKGPVQLLTRQPTEGKQ
                     KGGGLRFGEMEGEALVAHGAAMTLQEKLMDDADEMNIMVCEDCGIPAVDDKIRNKRYC
                     PVCNGTNVHQVKISYGFKLLLDELKAMGVYPKLNLGDKID"
     gene            4203..6794
                     /locus_tag="PLK55_03375"
     CDS             4203..6794
                     /locus_tag="PLK55_03375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HOZ35998.1"
                     /translation="MNTSKKIRSIEFGVLSPDMIRKMSAVEISRADTYDKDGYPIERG
                     LMDPHLGIISPGLRCKTCGQTMKGCPGHFGSLELIRPVIHPKFGEKLYCVLASTCQEC
                     GRMLLEEEEINELMLISEDTDKCVKEILNRVKGEKKCPHCKAIVNKIALDKPTNFFKN
                     GERLYPAEILDWLVKIPDKDLFLFGYSDKLKPQWFILSVLPVSPVSIRPSLSLENVIT
                     SEDDLTYKLLDIVRINIRLQENINAGAPQIIIEDLWDLLQYNITTYIDNNTAGVPPAK
                     QRSGRPLKTIAQRLKGKKGRFRFNLIGKRVNASARSTITPSVDIKFNELGIPEYVANT
                     LTVPEKVTAWNKDKLKEYILKDKVAYLLTEKGLRKIINPENKEELAEQLTEGMTVKRK
                     LQDGDIVIFNRQPTLHRVSMMGHYAKILPGRTFRLNPISCDPYNADFDGDDMNLHVPQ
                     SEESYTEVKELMIADKHVISIRTGKPVIAPDHDLISGAYLLTREKTEFSRKEAMALLY
                     SIGITELPTADRGRGYYSGKLIFSQILPKGLNIEYKNKLCSIVSKAKDCKKCKKEKCA
                     YDCYLKIEKGKIISGVMDEKMDMHKGLIETIYRYYGAEELANFYYKFSKLVFANIAKQ
                     GLTVALDEYKASEELQKEIRAKVKGMIASSNSIIKKYEDRSLPLIPGKNLEETFESEI
                     LKVTGEMKDKVSDKILELKLDELFSEVKKANNNTALVASIGGGRGRILNVVNMLGLWG
                     QVTVRTGRPKSGFSERLLSSSPKGSNQVTDYGFVQSNFFDGMSPREYFCHAIGGRQGE
                     IDTGVATKVSGYLYRRLSNALKDLVVNDELKVTSADGQVIQFMYGEDGLSPEKAYLGK
                     NINFFNE"
     gene            6826..7998
                     /locus_tag="PLK55_03380"
     CDS             6826..7998
                     /locus_tag="PLK55_03380"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HOZ35999.1"
                     /translation="MKALPIAIVEKVEKYATMKKLSNDKKKKLLDVVTKRYNDGLVVP
                     GDAVGLIAAQSIGEPGTQLTLRTKHYAGSLEVSVGQGIQRVIEIVDARSQARYPSMKI
                     YLDRNKLKREKDVDEFCKNLIDVKVGDVGSFVEDFSEKLVSFKFDTEKAEILGVNQEE
                     VMDSIYEKIEDTYDSKRFTNNKQQINFHFSNKTPLYNIRKAILKWNKIPLYGVKGIEK
                     AVVIEENGEKLIQTKGSNLKEVLKTEGVDTYKTKTNDIFEIYKAFGVEAAREAIVSEL
                     KYTFDVNGIVINIRHIYILADMMTSSGVIQGVVRTGITGKKKSPFARAAFEETIKHIV
                     AAAFNGEIEGLVGITENIIVGQPISAGTGKVRLTLDAKGLEKMIKAKALQDKKKEE"
     assembly_gap    8132..8231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8232..8534
                     /locus_tag="PLK55_03385"
     CDS             <8232..8534
                     /locus_tag="PLK55_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36000.1"
                     /translation="KSSILEVINEKDSDKKEDKKLIAKKAKKEDKKNAKKDKKAKKDE
                     DGDYSEDELKLKKKRDEKRAARGTKVKSETEEIKEEDEVPILEDKMFKDIIKIKKK"
     gene            8593..9018
                     /locus_tag="PLK55_03390"
     CDS             8593..9018
                     /locus_tag="PLK55_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOZ36001.1"
                     /translation="MANGEFSARKLIKDRKKWTKKDPRTKRKLKVDKKEGLMRGAPQA
                     RGIVLDKKGITARQPNSGIRKCVRVQLIKNGKELTAFAPKDKAIKFIDAHDQVTVEGI
                     GGRKGGPIGDLWGVRYKVVKVNDQSLEMLRKGKKEKTKR"
     gene            9029..>9627
                     /locus_tag="PLK55_03395"
     CDS             9029..>9627
                     /locus_tag="PLK55_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOZ36002.1"
                     /translation="MKLFDKWETKGINFKDAGLAKYMNLEDKVIPHSFGATNKEPSRK
                     TKINVVERLVNKMMRSGQGKKKLSGKFVRHRMGCGKKEQMLKTVEEAFEHIHKVTKEN
                     PVQVLVTAVEYSAPREDITKLQKGDITYTQSVDLAPIKRLDEALKNISLACFKNTYKS
                     KKEPAAALAEEIMDAARNDQKSFSVKRKDEIERIAQGSR"
BASE COUNT         3888 a         1238 c         1829 g         2572 t
ORIGIN      
        1 aaaaagaagg aaaagctata aaaaaaagaa ttgatgaact tacagataaa gaaaaagagc
       61 tatttaaaag cataatttcg cagaaaaaaa tagataaatc acaaaaaaca aagaaacgtc
      121 ccgaagtaac tagaaaaaga gaaaaaaaga aataaaacaa agtatttata aatattttgt
      181 tattataaat attatatgct cctgtctagt ttagctatat ttataatttt tacaaaaaac
      241 agtaagatta caggcgattt ttataaatat aagggcgtta attcaagctt aactatcagc
      301 ccgtattaga aaaagattgt ctagcgttag ctatcggcaa acaacttaaa gacagaaaag
      361 caaaaacatt tacaattaaa ggtgtaaggt tatgccagga ttaaaaacag atgtcggttt
      421 agaagatgat tatagctttg atgatataga tatcaaagcg ccaaaggaag aagttgaaga
      481 agaagaaaaa gaagagaaag ttacttttga tattcttgat gatgacgaag aggaagaaac
      541 aaaaggcaag ggcaaaagaa agaaagtaaa gaaaagagca agaaaagtaa aagaagaaat
      601 aaagcattct tatatcccta agcatgaaat tgtttcgggc gacgaattag aaaaacttat
      661 ttctgaaaaa aatataaatc cagaaaaact acctttaatc tatattgctg atccaggcat
      721 cgctcactta gaagtaaaag ttggagatgt gattagaatt gcaagaaaca ataaaaagat
      781 tggcgaagtt atttactata gaagagtaat ttctatatga ttttttaatt ctaaaattaa
      841 agataatatt tataaaagtg tgaaagatta tgaaaagttt agataaatgg ccagtaattg
      901 agtcttatta tgacttgtat agtcctgttc aacagcaaat caattctttt aatcagttta
      961 ttgatgaaaa agtttcagag attgttgaag aaaatggatt tattagttcc acaaataatg
     1021 ttgatgttac attatcaaat gtagaaatca caaacccaca gattgttgaa gcagacagaa
     1081 ctgcaaaatt tagattaatg ccaatggaag caagacttag aaacctaaca tactctggtt
     1141 caatttattt agatattaag ctttacagaa aaggagttct tcaagatgag aaaagagcat
     1201 atgttggaga aatgccaatt cttgtaaaat caaacagatg taacctttat ggtttatctc
     1261 cagaagaatt aatagaaaat ggagaagacc caatggacag gggaggttac atgattgtta
     1321 atggttcaga aaaagcacta atcactcaag aagttctagc atttgataga attctactta
     1381 acgatacaga agaagtaatt aatgcacaag tgatttcagt taaaggagca tttaaaggaa
     1441 aagtaagaat ctcaagaaca caagacggat tatttacaat ttcgtttcca tcttctccaa
     1501 gaaaattaat gcttgttgaa ttattaaatg caattggtta cacaaaatca actcatattc
     1561 tagatctttt cccaaaggac caaattattc aaaatgaaat tcttcttaat cttgataaag
     1621 tggaattaca atcagaagaa gaagctttag acaagattgg aagatcagtt gcaccttctc
     1681 agacaaagac ctatagacta agaagagcaa aagaagtaat tgattcgttc ttattgccac
     1741 acctaggtca agaaaaagat gcaagaacaa tgaaggcaca cttccttgta gcaatgtctg
     1801 ttaagattat tgaaaaagca tataatttaa gaacacaagc agacaaagac cattatgcta
     1861 ataagagact tgagctttct ggaaagttaa tggaaaatct tttcagattc tcttttaaac
     1921 attttacaaa agatcttaaa ttccagattg atagaacaat ttcaagacac agaaaattaa
     1981 atattaatac agtgattaga ccagatgcaa ttactgaaag aataatgttt gcagtaagca
     2041 ctggaaattg gatagggcaa ttaacaggtg ttagtaaata catgtcaaga gataactttt
     2101 tgacatcaat tacagatcta agaagagtga aatcaacttt agattcatca agagaacttt
     2161 acgaagcaag agatgttcac ggaacacact gggggagact ttgcccaatc gaaacaccag
     2221 atggaccaaa ctgtggtctt gttaagaatc tttcaatgat ggcaaagatt acaacagaca
     2281 ctgatccaga aggaattaga agaatattaa agaatgagga cgtcacctct gattagaatt
     2341 atttttaaaa gtgaaataat atggcaaaat caagagcaaa aatttatgtt aatggtggaa
     2401 ttgttggatt tcacgatgac cctgaaaaat taagagcaga tttacttaaa aaaagaagat
     2461 ctcttcagat tgatccgcaa ataaatattg catttaatga aacaacaaat gaagtttata
     2521 ttaacactga tgctggaaga gtacaaagac cagtaattat tttagataaa gggcaatcaa
     2581 gagtaacacc agaaattgta gaagacttaa gatcaaagaa attatcttgg caagatcttc
     2641 tttctaaagg aattgttgag tttttagatg ctgaagaaga ggaaaatgca tatattgctt
     2701 taacagaaga tgagattact ccagatcaca cacacttaga aatttcagga acagctatct
     2761 ttagtattgt ttcatcaatg ataccattta ttgagcacaa catggctggt aaagcaattc
     2821 atggtgctaa actttacaag caggcaattg gaataccatc atctaattat aatttaagat
     2881 ttgatacaga aatgcattgt ttgtattatc cacaaaaacc tttagtttac acaagaacat
     2941 caaagctttt acatatggaa aaaagaccaa tgatacaaaa tgcaattgtt gcaatcatgc
     3001 cttacaaagg tttcaatatg cttgatgctt ttattattaa tcaaggcgca atacaaagag
     3061 caatgtcaag atcttcatat tatagatcat attctcaagc agaaatcaga tatccttctg
     3121 ggcagacaga caaattttgt attcctacag aagaagcaag cggatttaga ggaacagatg
     3181 cttatgcaaa tcttgatgaa aatggaattg taatgctaga atcaaaaatc tcagaaggcg
     3241 gagtagttat tggtatgacg tctcctccaa gatttgttga agaagtttca gagtttggag
     3301 ttgttacaga agaaagaaga gatacttctg tttgttctag aaaacattta gatggttatg
     3361 ttgacagagt aatgatttct gaaacaattt ctggaactaa attatgtaaa gtaagaatta
     3421 gaacttatct tgaaccacag gttggagata aatttacaag tcatcacgca cagaaagggg
     3481 ttgttggtgc agtaataaga gaagaagata tgccttttac aagtcaagga ataaaaccag
     3541 actttatttt aaatccgcat tccattcctt caagaatgac tttaggtcat ttgtttgaaa
     3601 gcattgccgg taaaactgtt gcagcaagtg ggaaggcaat tgatggaact cctttctgta
     3661 atgataaaga cgaagttttt aaattattac aagaatatgg atttagacca gatggtaaag
     3721 aaacctttta tgatggtaag accggagaaa agatagagat gccaattttt gttgggcctc
     3781 ttggttttga aagattacaa acacacttag ttgttaatag aatacaagca agagataaag
     3841 ggccagtaca attattaaca agacaaccaa ctgaaggtaa gcaaaaaggc ggagggctaa
     3901 gattcggtga aatggaaggc gaagctttag ttgctcatgg tgctgcaatg acattacagg
     3961 aaaaattaat ggacgatgca gacgaaatga atatcatggt ctgtgaagac tgtggaattc
     4021 cggcagttga tgataagata agaaacaaaa gatactgtcc agtatgtaat gggacaaacg
     4081 tacaccaagt aaagatcagt tatggtttca agctattgct tgatgaacta aaagcaatgg
     4141 gagtatatcc aaagttaaat cttggagata aaatagacta gagcatttaa aaggtagata
     4201 agatgaacac atcaaagaaa ataagaagca ttgaatttgg agtattaagt cctgacatga
     4261 ttagaaagat gtctgctgtt gaaatctcaa gagctgatac ttatgataag gatggttatc
     4321 caattgaaag aggattaatg gatcctcacc ttggaataat ttctccagga cttagatgta
     4381 aaacttgtgg acaaacaatg aaagggtgtc caggacactt cggttcttta gaattaatca
     4441 gaccagtaat tcatccaaag tttggagaaa aactatattg tgttctagct tcaacttgtc
     4501 aagaatgtgg aagaatgctt cttgaagaag aagaaataaa tgaattaatg ctaatatcag
     4561 aagacacaga caaatgtgtt aaagaaatct taaacagagt aaagggagag aagaaatgcc
     4621 cacactgtaa agcaatagta aataaaattg ctcttgataa accaacaaac tttttcaaga
     4681 acggagaaag actatatcct gcagagatac ttgattggtt agtaaagatt ccagataaag
     4741 atttattctt gtttggttat tcagacaaac taaaaccaca gtggtttatt ctttcagttt
     4801 taccagtctc tccagtttca attagaccat cgttgtcttt agaaaatgtt attacttctg
     4861 aggatgattt aacctataag cttctagata ttgtaagaat aaatattaga ctacaagaaa
     4921 atattaacgc tggagcccca caaataatta ttgaagatct ttgggatctt ttacaataca
     4981 atattacaac atatattgat aataacactg ctggcgttcc acccgcaaag caaagatcag
     5041 gaagaccatt aaaaacaatc gctcaaagac ttaaaggaaa gaaaggaaga tttagattta
     5101 accttattgg taaaagagta aatgcttctg cgcgttcaac aatcacacca agtgtagata
     5161 ttaaatttaa tgaactaggt attccagagt atgttgctaa cactctaact gttccagaaa
     5221 aagtaacggc ctggaataaa gataaattaa aggaatatat acttaaagat aaagttgcgt
     5281 atcttctaac agaaaaaggt ttaagaaaga taataaatcc agaaaataaa gaagaattag
     5341 ctgaacaatt aacagaagga atgactgtta aaagaaaact acaagatgga gatattgtta
     5401 tttttaacag acagccaaca cttcacagag tttcaatgat ggggcattat gctaagattc
     5461 ttcccggaag aacctttaga cttaacccaa tatcttgtga tccatataac gctgactttg
     5521 atggagacga tatgaatctt cacgtaccac aaagtgaaga atcctatact gaagttaaag
     5581 aacttatgat tgcagataag catgtaataa gcattagaac cgggaaacca gttattgctc
     5641 cagaccacga tctaatatct ggcgcttatc ttttaacaag agaaaaaact gagttttcaa
     5701 gaaaagaagc aatggcatta ttatattcta ttggaataac agaacttcct acagcagaca
     5761 gagggagggg atattattct ggtaaattaa tatttagtca gatacttcca aaaggtctaa
     5821 atattgaata taagaacaaa ctttgtagca tagtttctaa agcaaaagat tgtaaaaaat
     5881 gtaaaaaaga aaaatgtgca tatgattgtt atctaaagat agaaaaagga aagataatct
     5941 caggagtaat ggatgaaaaa atggatatgc acaaaggttt gatagaaaca atatatagat
     6001 attatggtgc agaagaactt gcaaacttct attataagtt ttcaaaacta gtatttgcaa
     6061 atatcgctaa acaaggttta acagttgctc ttgatgaata taaagcaagt gaagaattac
     6121 aaaaagaaat aagagcaaaa gtaaaaggca tgattgctag ttcaaattca ataattaaga
     6181 aatatgaaga tagaagctta ccactgatac cgggaaagaa tcttgaagaa actttcgaaa
     6241 gtgaaattct aaaggttact ggtgaaatga aagataaggt ttcagataaa atcttagaat
     6301 taaagcttga tgaattgttt tcagaagtaa agaaagcaaa taacaacacc gctcttgttg
     6361 caagtattgg tgggggtcgt ggaagaatat taaatgttgt aaacatgctt gggctttggg
     6421 ggcaagtaac tgttagaaca ggaagaccaa aatcaggatt ttctgaaaga ttattaagtt
     6481 caagtccaaa aggcagtaat caagtaacag attacggttt tgttcaaagt aatttctttg
     6541 atggaatgag tccaagagaa tatttctgtc acgctattgg tggtagacaa ggtgaaattg
     6601 acacaggtgt tgcaacaaaa gtatctggtt atctttacag aagattatca aatgctctaa
     6661 aggatcttgt tgttaatgat gagttaaagg tcacctcagc agatggacaa gtaatacagt
     6721 ttatgtacgg agaagatggt ttatctccag aaaaagcata ccttggaaag aacataaatt
     6781 ttttcaatga gtgaataatt taaatgaaat ttaaaaagtg ataaaatgaa agcattacca
     6841 attgctattg tagagaaagt agaaaaatat gcaactatga aaaaactaag caatgataag
     6901 aagaaaaaac ttcttgatgt cgttacaaaa agatataatg atggcttagt tgttccagga
     6961 gatgctgtgg gattaattgc tgctcagtca attggtgaac ccggaacaca gctaacactt
     7021 agaacaaagc actatgccgg ttctttggaa gtttctgttg gtcaaggaat acaaagagta
     7081 attgagattg ttgatgcgcg ttcacaagca agatatccat ctatgaagat atatctagat
     7141 agaaacaaat taaagagaga aaaagacgtt gatgagttct gtaaaaacct aattgatgtt
     7201 aaagttggag atgttggttc ttttgttgaa gacttctcag aaaaattagt ttcatttaaa
     7261 tttgatacag aaaaagcaga gattttaggg gtaaatcaag aagaagtaat ggattcaata
     7321 tatgaaaaaa tagaagacac atacgattca aaaagattta cgaataacaa gcaacagata
     7381 aatttccatt tttcaaacaa aactccatta tacaatatta gaaaagcaat attaaaatgg
     7441 aataagatac cactatatgg tgtaaaaggt atagaaaaag cagttgtaat tgaagaaaac
     7501 ggagaaaagc taatacagac aaaaggatca aatcttaaag aagttttaaa aacagaaggc
     7561 gttgatacct ataaaacaaa gacaaatgat atctttgaaa tctacaaagc gtttggtgta
     7621 gaggctgcta gagaagcaat agtttctgaa ttaaaatata catttgatgt taatggaatt
     7681 gttattaata ttaggcacat ttacatacta gcagacatga tgacttcttc aggagtaata
     7741 caaggagttg taagaacagg tattactgga aagaagaaaa gtccatttgc aagagctgca
     7801 tttgaagaaa caattaaaca tattgttgca gcagcattta acggcgaaat tgaaggccta
     7861 gttggtataa ccgaaaacat aattgttggt cagccaattt ctgcaggaac gggaaaggtt
     7921 agattgactt tagatgctaa aggccttgaa aagatgataa aagccaaggc tttacaggat
     7981 aagaaaaaag aagagtagtt tataatattt ttgtagctaa aatatatata attttagata
     8041 ttaaaaaaga tgtgcaagac aaagtaaatt aaataagagg atgaaacaat atgaattgga
     8101 caagattcaa tcaagaatta agaaacacaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn naaatcttca atcttagaag taattaatga aaaagattct gataaaaaag
     8281 aagataagaa attaattgca aagaaagcaa agaaagaaga taaaaaaaat gctaaaaaag
     8341 ataaaaaagc aaagaaagac gaagacggag attattcgga agatgaatta aaattaaaga
     8401 agaaaagaga cgagaaaaga gcagcaagag gaacaaaagt taaatctgaa acagaagaaa
     8461 ttaaagaaga agacgaagtt ccaatattag aggataagat gtttaaggac attatcaaaa
     8521 ttaagaaaaa ataattgagc ctttctttaa aagaaagact cggtaaagaa actaaactaa
     8581 ggtgaaatga ttatggcaaa tggagaattt tcagcaagaa aattaataaa agatagaaag
     8641 aaatggacta aaaaagatcc aagaactaaa agaaaactaa aggttgataa aaaagaagga
     8701 ctaatgagag gggctcctca agcaagagga attgttcttg ataagaaagg aataaccgca
     8761 agacagccaa actctggtat tagaaaatgt gttagagttc agttgattaa aaatggaaaa
     8821 gaactaaccg catttgctcc taaagataag gcaattaagt ttattgatgc tcacgaccaa
     8881 gtaactgttg aaggtatcgg tggaagaaaa ggcggtccga tcggagatct ttggggtgtg
     8941 agatataagg ttgttaaggt taatgaccaa tcacttgaaa tgttaagaaa aggtaagaag
     9001 gaaaagacta agagataggt gttaaaaaat gaaattattt gataaatggg aaacaaaagg
     9061 aataaacttt aaggatgcag gccttgctaa atatatgaat ttagaagaca aggttatacc
     9121 acattcattt ggtgcaacta acaaagagcc ttctagaaaa acaaaaatca atgttgttga
     9181 aagacttgtt aataagatga tgagatcagg acaaggaaag aaaaagcttt ctggtaagtt
     9241 tgtaagacac agaatgggtt gtggaaagaa agagcagatg ttaaaaactg ttgaagaagc
     9301 atttgagcac atccacaaag ttacaaaaga aaatcctgtt caagttcttg taactgcagt
     9361 tgaatattct gcaccaagag aagatatcac aaagcttcag aaaggggata tcacttacac
     9421 acaatcagtt gatcttgctc caatcaagag acttgatgaa gcattaaaga atatttcact
     9481 tgcgtgtttt aaaaacacct ataaaagtaa gaaagaacca gcagcagctt tagcagaaga
     9541 aataatggat gctgcaagaa acgaccaaaa gagtttttca gtaaaaagaa aagacgaaat
     9601 cgaaagaatt gcacaaggtt cacgtta
//
LOCUS       DAOOON010000023         9508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_8935_length_9508_cov_20.180154, whole genome shotgun sequence.
ACCESSION   DAOOON010000023 DAOOON010000000
VERSION     DAOOON010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8935_length_9508_cov_20.180154"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..373
                     /locus_tag="PLK55_03400"
     CDS             74..373
                     /locus_tag="PLK55_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36003.1"
                     /translation="MKKENTKIMLWAVVALVLGIVIGAVITNATIGNAKSIYTQDKNI
                     TAKTLNPNPEFYNLSYEDCLEVGLPAFDPPNGGAEGPWNYVGCVIDGFFDEIEID"
     gene            636..1940
                     /gene="asnS"
                     /locus_tag="PLK55_03405"
     CDS             636..1940
                     /gene="asnS"
                     /locus_tag="PLK55_03405"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOZ36004.1"
                     /translation="MEKKYLSVKGVLESKTEDISIRGWVYRIRDQKDNIFVTIRDETG
                     IIQLVASKDKLKPEEFQTAKALGIESSIIVHGKLRKEERSPQGYEVDIDSIELVGKSE
                     NYPISKDFSEEFLLDVRHLWLRSRKMTSILKIRSTVIGAIHEYFRKEGFYEYQSPILQ
                     SVQCEGGSSLFKVDYFGEEGVFLAQTWQLYAEPAIFSLGKIYTIAPSFRAEKSKTSRH
                     LTEYWHAEMEGVWIDFEMLQDYGEGLIKHVIKTVLSKHKEDLDILKRDTKLLETAISK
                     PFIRMTYTEVLKLLKEKADMEVEWGKDLRTIEEDKLSKMHDTFTIVTRYPKVVKAFYM
                     KEDPKDSSVVLGFDCIGPEGYGEIIGASEREPDLEKIKQRLLDDGEDPKHYEFYLDTR
                     RYGSVPHAGFGLGVERLIAYITGVDNIKDTIPFPRTMVRFKP"
     gene            2072..2144
                     /locus_tag="PLK55_03410"
     tRNA            2072..2144
                     /locus_tag="PLK55_03410"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2105..2107,aa:Asn,seq:gtt)
     gene            2201..3190
                     /gene="asnA"
                     /locus_tag="PLK55_03415"
     CDS             2201..3190
                     /gene="asnA"
                     /locus_tag="PLK55_03415"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOZ36005.1"
                     /translation="MDYFQTQEAIQFIKTDFEKNLSKKLNLLRVSAPKFLIANTGLLD
                     DLAGTQKPVSFKVKFLKEDIEIVHSLAKWKRLILGEFKFPIGVGLYTDMDAIRKDEDV
                     DSTHSVYVDQWDWEKVITKEQRNIKFLKKIVKQIYSSMYKTKLKLNKKYKQLEYTIPK
                     DIHFIHSVDLQKLYPKLTPKEREDKITEQHGAVFIIGIGADLSDNKPHDIRATDYDDW
                     STIENGKPGLNGDILVWDKVSGKALELSSMGIRVDKDALLFQLKEKNEIHKLKLDYHK
                     KIISNKIPLSIGGGIGQSRLSMFILEKAHIGEVQSCVWPKGLITKFKNKGVIL"
     gene            3255..3740
                     /locus_tag="PLK55_03420"
     CDS             3255..3740
                     /locus_tag="PLK55_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36006.1"
                     /translation="MPAIAARKPISRKPMEIKNSILRQRELAKVSTKKRELKRENLSK
                     EISEFDKKVNRLPENKRPIVQKQLLRKLEARHNYIQANIEKISQNLHRYKFNEKNITF
                     LETLRKQEVKRTNNNQYQMWDYAEQKLKAGYKPIELLKAGYNVGEVTAGVFRLETKRK
                     A"
     gene            3737..4048
                     /locus_tag="PLK55_03425"
     CDS             3737..4048
                     /locus_tag="PLK55_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36007.1"
                     /translation="MNKAICYSNLTLSHKKHPLQFTVGIGFQGKLINGRVKGIDLNKE
                     RKTLEDFNAKIRDKELFVLIKGYKGMESLANFYFKELKNKKIDFIIIKESNTEEIIIE
                     K"
     gene            4100..4600
                     /locus_tag="PLK55_03430"
     CDS             4100..4600
                     /locus_tag="PLK55_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36008.1"
                     /translation="MVKLKAVVDSSFWINLNKTDLVKYLFDYFDLYFTEKVEDELYDY
                     KDTRASVPKDLDSFIQLKKLDFVQVKNPKTVSKKLFDTLSNYSGEIYTIALAEELNAI
                     VLTDDSGPLKYCNRYGIISITTPTFILKLFKDRIITKEIAKQKINLIKKSIRKEIIGE
                     ALKQLK"
     gene            4609..4959
                     /locus_tag="PLK55_03435"
     CDS             4609..4959
                     /locus_tag="PLK55_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOZ36009.1"
                     /translation="MKKYNVKKVKSFRLSDNALASISYISKAENINESEVTRIVIDKG
                     IKEYLFESAVNEYNTKKICLSEAAKIAGLSKRDFMKELEKRGIPLNMDLEDFRYSFES
                     MERILKKIDGKSKK"
     gene            complement(4962..6407)
                     /gene="cysS"
                     /locus_tag="PLK55_03440"
     CDS             complement(4962..6407)
                     /gene="cysS"
                     /locus_tag="PLK55_03440"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOZ36010.1"
                     /translation="MAIKLYNTATKKKEVFKSVNPNQLKIYVCGVTIYDDVHLGHAFS
                     YINYDILINYFKYFLDYDILYVRNITDVGHLTDDGLSDTGEDKIQKRASERKVHPMEL
                     VYKYSHRMWKHFDALKISRPNLEPTASAHILEMQEWIKKMLKNGFAYEVNGNVYYDIS
                     KFKDYGKFANKNLEELDKNTRFENDPNKRNIGDFALWIKAKPEHILKWPSPWGEGYPG
                     WHLECSVMGTKYLGERFDIHGGGIEHLFPHHQNEIAQNYGYYGHDVVNYWVHTAMLMV
                     EGKKMGKSLGNFITVEDFLKKHSAESLRYLFSTGNYRKPQNYTEKAIEDSEIAIDRLN
                     KYIKRLQAVTNNKNTNFGKEIIENAIENFKNSMNDDLNTPSAWASIWFLVKEANRLMD
                     LQQLTEEESHKILDFLQKVNQVFKVFNFEQEVPKEKQVIQRKEIEEQINKRNQLRKEK
                     KWEESDQIRLNLEKKGVVLSDTPTGTTWHYE"
     gene            6478..7491
                     /gene="kae1"
                     /locus_tag="PLK55_03445"
     CDS             6478..7491
                     /gene="kae1"
                     /locus_tag="PLK55_03445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HOZ36011.1"
                     /translation="MVKAKICLGIESTAHTLGVGIIDSNGKILANRKRQFVAKPGSAT
                     GLKPFDVSIHHYSCAVDVVKEALEESKLKIKDIDVISFSQGPGLAPCLTVSATLARAL
                     ADKYNKPIIGVNHCVSHIEIGKTILKCKDPLVIYTSGANTQIIAKDNGYYKILGETLD
                     IGIGNLFDSFARDIGYGFPGGPVLDKIYMENKERHYIELPYSVKGMDLAFSGLQTAAR
                     KKIATEKKEDLVYSFMHTAYAMLLEVTDRALSYTKKKEVIVIGGVAASKALKEMLEKL
                     CKQNKVKLYIPPAIVCLDNGLLIADLGVKHFINTYKGKGGMKLEETLINPKFRTDQVK
                     IDW"
     gene            7496..8503
                     /locus_tag="PLK55_03450"
     CDS             7496..8503
                     /locus_tag="PLK55_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36012.1"
                     /translation="MENKDLVYYFFKKKLNENKYNLKYGTKPDLFFNLDIDSAKPYLS
                     KVDIKEQKEIVRIIENFNITQLSSVLENIRYNLEHKREFNSFLRDTRDIQKGRFLKKP
                     RARVDSPVCIDTYIGFMRALKEGTLYTAKLKIDGKLKRVFIKTPIGNANDLNLDFGFS
                     RLAIDPETLNVYSFINNRAGRINETKIKKVFSKTDLDRLALEYENQKTAGIARESYLN
                     FRKELLEKMDAVYGIERIIDTYLRMGGKIDSQKYKNKDFKEELIRHLEERFGTEKLLN
                     WVSKIKNPKEAAKLKLEILESLGKYHGQEYRAHYSAEYNRAKHQKIQKLRPKKAIKRL
                     I"
     gene            8591..8938
                     /locus_tag="PLK55_03455"
     CDS             8591..8938
                     /locus_tag="PLK55_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36013.1"
                     /translation="MKAKFHIVKKVADMEHPKNNSKTEKDIEVEVDEQFELEGGIYVF
                     KLLAARANGAQVEYDRHYSIMNEHRGYEFNTIFYMGEPKIVTSLWGKNQITYTITYNG
                     FGSSSEENTESFE"
     gene            8983..9330
                     /gene="pth2"
                     /locus_tag="PLK55_03460"
     CDS             8983..9330
                     /gene="pth2"
                     /locus_tag="PLK55_03460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012068149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HOZ36014.1"
                     /translation="MYKQVIIFRNDLKLGKGKIAGQVSHAAVEAYILTSKKDPSAVDN
                     WLMEGQKKVVLKIDSKKELLELYQSIKNKFPCVLIKDAAKTQLPEPDITCLGIGPIKE
                     TEIDQFTGKYKLL"
BASE COUNT         3691 a         1288 c         1576 g         2953 t
ORIGIN      
        1 atatatatat atatcggttc tcttttttta tatttattta attaattaac ttaaaaaagg
       61 agatgaaatc aaaatgaaaa aagaaaatac aaaaataatg ctttgggcag tagttgcttt
      121 agttcttggt atagttattg gtgcggtaat tacaaatgca acaattggaa atgctaaatc
      181 aatttataca caggataaaa atataactgc taaaacatta aatcccaatc cagagtttta
      241 taatctaagt tatgaggatt gtttagaagt tggtcttccc gcctttgatc ctccaaatgg
      301 cggtgcagaa gggccctgga attatgtagg gtgtgtgatt gatggttttt ttgatgagat
      361 agagatagac taaaatatat ataactagaa gagaatttca ttctctttta taaacttttc
      421 ttttcattaa gatatttatg atatacaaaa tagttttaga ggattctgat tataaatcaa
      481 agttttgtat aattatcaat aactttaatg gccagtaggc cactacttta tctattcgca
      541 ttgtaactta ttgtcaaaat cttgacataa gtggtttatt tttaatatat ttgtttatca
      601 ttatttaata attaaaatgg aagtgaatta aaaaaatgga aaaaaaatat ttaagtgtta
      661 aaggggtttt agaaagtaaa acagaagata tatctattcg aggatgggta tatagaatta
      721 gagatcaaaa agataatatc tttgtaacaa ttagagatga aaccggaatt atacagcttg
      781 ttgcttcaaa agacaagtta aaaccagaag agtttcaaac agcaaaagca ttaggaatag
      841 aatcttcaat aatagttcat ggaaaactaa gaaaagaaga aaggtctcct caaggttatg
      901 aagttgatat tgattcaata gagcttgttg gaaaatcaga gaactatcca atctctaaag
      961 acttctctga ggagtttctt ctagatgtta gacatctctg gcttagaagt agaaaaatga
     1021 caagcatcct aaagataaga tcaacagtaa ttggagcgat tcatgaatat tttagaaaag
     1081 aaggattcta tgaatatcaa tcaccaatac ttcaatcagt ccaatgcgaa ggaggttctt
     1141 ctctttttaa agttgattac tttggagaag aaggagtttt cttagctcag acctggcaac
     1201 tatatgctga gccagcaatc ttttctttag gaaagattta taccattgct ccatctttta
     1261 gagcagagaa atcaaagacc tcaagacacc ttacagaata ctggcatgca gagatggaag
     1321 gggtttggat tgattttgaa atgcttcaag attatggtga agggctaatc aaacatgtaa
     1381 tcaaaacagt actttcaaaa cataaagaag atcttgatat ccttaaaaga gacacaaagt
     1441 tattagaaac agcaatctct aaaccattca taagaatgac atatactgaa gttctaaaac
     1501 ttcttaaaga aaaagcagat atggaagttg aatggggaaa agatctaaga acaattgagg
     1561 aagacaagtt aagtaaaatg cacgatacct ttacaatagt tacaagatat ccaaaagttg
     1621 ttaaggcctt ttacatgaaa gaagacccaa aagactcaag tgtcgttctt ggctttgatt
     1681 gtattggccc agaaggttat ggagaaatta ttggtgcaag tgaaagagag ccagacttag
     1741 aaaagataaa gcaaagatta ctagatgatg gcgaagaccc aaagcattat gagttttatc
     1801 tagacactag aagatatggt tcagttccac atgcaggctt tggtttaggt gttgaaagat
     1861 taattgcata cattactggt gttgacaaca taaaagacac aataccattt ccaagaacta
     1921 tggttcgttt caaaccgtag ataaaaatat aattggttcg cgttgataat cgtgcataag
     1981 cacgaaatcc attgcgcacg aatccataaa aaccatttgg tttttagggc accagaaaat
     2041 atattttctg ggtgcgagcg aaaccgattc tgcctcagta gctcagtggt tggagcaatc
     2101 gactgttaat cgataggtcg taggttcaaa tcctacctgg ggcgtttaaa aggagatgaa
     2161 taacttatga aaaagtatct acccaacata taaatcgaaa ttggattatt ttcaaacaca
     2221 agaagcaatt caatttataa aaactgattt tgaaaaaaac ttatcaaaaa aactaaacct
     2281 tttgcgtgta agcgcaccga aatttttaat tgcaaacact gggctgcttg atgatcttgc
     2341 gggaactcaa aaacctgttt cttttaaagt taaattttta aaagaagata tagaaattgt
     2401 acattctctt gcaaaatgga aaagattaat acttggagaa tttaaatttc caataggtgt
     2461 gggactatat acagatatgg acgccataag aaaagatgaa gatgttgatt ccacacatag
     2521 tgtttatgtt gatcaatggg actgggaaaa agtaattaca aaagaacaaa gaaatataaa
     2581 attcttaaaa aaaatagtaa aacaaattta ttcaagcatg tataaaacaa aattaaagtt
     2641 aaataaaaaa tataaacaat tagaatatac aattccaaaa gatatacatt ttatacattc
     2701 agtagattta caaaagttat atccgaaact aaccccaaaa gaaagagaag acaagattac
     2761 agaacaacat ggtgcagttt ttattattgg tattggtgca gatttatctg acaataagcc
     2821 acacgatata agagcaactg actatgatga ttggtcaaca atagaaaacg gaaaaccagg
     2881 attaaacgga gacattttag tttgggataa agttagtggt aaagcattag agttatcctc
     2941 tatgggaata agagttgata aagatgcatt attgtttcaa ctaaaagaaa agaatgaaat
     3001 acacaagttg aaattggatt atcacaaaaa gattatttca aataaaatcc ctctttcaat
     3061 tggtggtggt atcgggcaat caagactttc tatgtttata ttagagaaag cacacattgg
     3121 agaagttcaa tcctgtgttt ggcctaaagg cttaataact aaatttaaaa ataaaggggt
     3181 tattttataa tttgaatttc tttttaaatt tataggttta taatattatt ataaaaacta
     3241 aaaaaggtga taatatgcca gcaattgcag caagaaaacc gatttccaga aaaccaatgg
     3301 aaattaagaa ttcaatttta agacaaagag aattagcaaa agtgtctaca aaaaaaagag
     3361 aattaaaaag agaaaactta tcaaaagaaa tttcagaatt tgataaaaaa gttaatagac
     3421 ttccagaaaa caaaagacct attgtacaaa agcagctttt aagaaaacta gaagcaaggc
     3481 acaattatat tcaagcaaat attgaaaaaa taagtcaaaa tctacacagg tataaattta
     3541 atgaaaagaa tataacgttt ttagaaaccc taagaaaaca agaagtaaaa agaacaaaca
     3601 ataaccaata tcaaatgtgg gattacgcag agcagaaact aaaagcggga tataagccaa
     3661 tagaactttt aaaagcagga tataatgtag gagaagtaac tgctggagtt tttagactag
     3721 aaacaaaaag aaaagcatga acaaagcaat atgttattcc aatctgacac taagtcacaa
     3781 gaaacaccca ttacagttta ctgttggtat tggttttcaa ggtaaactaa taaatggcag
     3841 agttaaaggc attgatctaa acaaagaaag aaaaactcta gaagacttta atgcaaagat
     3901 tagagataaa gaattatttg ttttgattaa aggctataag ggcatggaat ctcttgctaa
     3961 tttttatttt aaggaattaa aaaataaaaa aatagatttt ataattatta aagaaagcaa
     4021 cacagaagaa ataataattg aaaaataaac gtattacaaa aagcgactat ttttaaacat
     4081 tttgtaatac attatattta tggttaaact aaaagcagta gttgattctt cattttggat
     4141 taatcttaat aaaacagatt tagtcaaata tttatttgat tatttcgatt tgtattttac
     4201 agagaaagta gaagatgagc tttatgatta taaagacaca agagcaagtg ttccaaaaga
     4261 tctagattct tttatacaat taaaaaaatt agattttgta caagtaaaaa accctaaaac
     4321 cgtttcaaaa aaactttttg atactctttc caactactcc ggagaaatat atacaatagc
     4381 attagccgaa gaattgaatg caatagtttt aacagacgat tctgggccat taaaatattg
     4441 taataggtac ggaattatat caataacaac tcccacattt attttaaaac tttttaaaga
     4501 tagaataatt acaaaagaaa tagcaaagca gaagattaat ttgataaaga aaagcataag
     4561 aaaagaaata ataggagagg cattaaaaca attaaaatag gtgaaaaaat gaaaaaatat
     4621 aatgttaaga aagtaaaatc ttttagatta tcagataatg cattagcatc tataagctat
     4681 atttcaaaag cagaaaacat taatgaatca gaagtgacaa ggattgttat tgataaagga
     4741 attaaagaat atctttttga atcagcagtt aacgaataca atacaaagaa gatctgttta
     4801 agtgaagcag caaagattgc aggactttca aaaagagact ttatgaaaga actagaaaaa
     4861 agaggaattc cattaaatat ggatctggaa gattttagat attcatttga aagtatggaa
     4921 aggatattaa aaaaaattga tggaaaaagt aagaaataaa actactcata atgccaagtg
     4981 gttcctgtcg gggtatcgct taagacgacg cctttctttt ctagatttaa acgtatctga
     5041 tcagattctt cccacttttt ttcttttcgc aactgatttc gtttatttat ttgttcttca
     5101 atttcttttc tttgaattac ttgtttttct ttaggcactt cctgctcaaa attaaatact
     5161 ttaaatactt gatttacttt ttgtaagaaa tctaagattt tatgcgattc ttcttctgtt
     5221 aattgttgta aatccattaa tctgtttgct tctttaacta aaaaccagat gcttgcccaa
     5281 gcagaaggag tatttaaatc atcattcatt gagtttttaa aattttcaat agcgttttct
     5341 ataatttctt ttccaaagtt tgtgttttta ttattagtta ctgcttgtag tcttttaata
     5401 tatttattta gtctatcaat tgcaatttct gaatcttcaa ttgctttttc tgtataattc
     5461 tgtggctttc tgtaatttcc tgttgagaaa agatatctta gtgattctgc agagtgtttc
     5521 tttaaaaaat cctctactgt tatgaaattt ccaagagact tgcccatctt ctttccttca
     5581 accataagca ttgctgtgtg cacccaatag tttacaacat cgtgtccgta atatccatag
     5641 ttttgtgcaa tctcgttttg atgatgtggg aaaagatgtt ctattcctcc accatgtata
     5701 tcaaatcttt ctcctaggta tttagtgccc ataacagaac actcaagatg ccagccaggg
     5761 tagccctctc cccaaggcga tggccattta agtatgtgct ctggttttgc ttttatccac
     5821 aaagcaaagt ctccaatatt tcttttgttt gggtcgtttt caaatcttgt gtttttatca
     5881 agttcttcta gatttttgtt tgcgaattta ccatagtctt taaacttaga aatatcataa
     5941 taaacatttc catttacttc gtatgcgaat ccattcttta acattttctt tatccattct
     6001 tgcatctcaa ggatgtgtgc agaagcagtt ggttctagat taggccttga tatctttaaa
     6061 gcatcaaaat gtttccacat tctatgtgaa tatttgtaaa ccaactccat tggatgaact
     6121 tttctttcgc ttgcgcgctt ttgtatcttg tcttctcctg tatcagatag gccgtcgtct
     6181 gttagatgcc caacatcagt aatgtttcta acatataaaa tatcataatc taagaaatac
     6241 ttaaaatagt ttattagaat atcataatta atatatgaaa aagcatgacc taaatgcacg
     6301 tcatcataaa ttgtaacacc acaaacatat atctttagtt ggttaggatt aactgattta
     6361 aaaacttctt ttttcttagt tgcggtatta tacagtttta ttgccatatt ctttcacttt
     6421 ctaatataga aattaaacta tatagttatt aaaaatatat cttaattaga tatatttatg
     6481 gttaaagcaa aaatttgtct tggaatagaa agcacagctc acaccttagg tgttggtatt
     6541 attgattcta atggtaagat tctagcaaac cgtaaaagac agtttgtggc taaaccaggt
     6601 agtgctactg gactaaagcc atttgatgtt tcaatacacc attattcttg tgctgttgat
     6661 gttgttaaag aagctttaga ggaatcaaaa ctaaaaatta aagacattga tgttatttct
     6721 ttttcacaag gtcctggtct tgcgccatgt ttaacagttt ctgcaacttt agcacgggct
     6781 ttagcagaca aatataataa accaataatt ggggtcaatc attgcgtttc gcatatcgag
     6841 attgggaaaa ccattctgaa atgtaaagac cctttagtga tttatacatc aggagcaaac
     6901 actcaaatta ttgcaaaaga caatggttac tataagatac ttggagagac acttgacatc
     6961 ggaattggaa atcttttcga ttcttttgct agagacattg gttatggctt tcctggtggg
     7021 ccagttcttg ataaaatcta tatggaaaat aaagaaaggc attacattga actaccatat
     7081 tctgttaaag gaatggactt agcattctct ggattacaaa ccgctgcaag aaaaaagatt
     7141 gcaacagaaa aaaaagaaga ccttgtttat tcttttatgc acacagctta tgcaatgcta
     7201 ttagaagtaa cagacagagc tttatcttac acaaagaaaa aagaagtaat tgttattggt
     7261 ggagttgctg ctagtaaagc cttaaaagag atgctagaaa aattatgtaa gcaaaacaaa
     7321 gttaagcttt atatccctcc agcaattgtg tgccttgaca atggtttatt gatagcggat
     7381 cttggagtca agcattttat taatacttac aaaggtaaag gcggaatgaa actagaagaa
     7441 actcttatca acccaaagtt tagaacagac caagtaaaga tagactggtg aaaaaatgga
     7501 aaacaaagat ttagtttatt atttttttaa aaaaaaacta aatgaaaata aatataattt
     7561 aaaatatggt acaaaacctg atcttttttt taacttggat atagattctg caaaacctta
     7621 tttatcaaaa gtagatataa aagaacaaaa agaaattgtt cgaattattg aaaactttaa
     7681 tattactcag ctttcttcgg ttttagaaaa tattagatat aatctagagc acaaacgaga
     7741 atttaatagt tttttaagag acacaagaga tatacaaaaa ggcagatttc ttaaaaagcc
     7801 aagagcaaga gtagattcgc cagtttgtat agacacttat attggattta tgagggcact
     7861 aaaggaaggc acactatata ctgcaaaact aaagattgat ggcaaattaa aaagagtatt
     7921 tattaaaacg ccaataggaa atgcaaacga cttaaattta gattttggat tttcaagact
     7981 tgcaatcgat cctgagactc taaatgttta ttcttttata aataatagag ctggcagaat
     8041 aaatgaaaca aaaataaaga aggttttttc taaaacagat ttagaccgct tggctttaga
     8101 atatgaaaat caaaaaacgg caggtattgc tagagaaagt tatttaaatt ttagaaaaga
     8161 gcttttagag aaaatggatg cagtatatgg tattgaaaga attattgata cctatttaag
     8221 aatgggcggc aaaatcgact ctcaaaaata caagaacaaa gactttaaag aagaactaat
     8281 aaggcatctt gaagaaagat ttggaactga aaaattatta aattgggtct caaaaatcaa
     8341 aaatccaaaa gaagcagcaa aattaaagct tgaaatatta gaaagcctag gaaaatatca
     8401 tggacaagaa tatagagcac attatagcgc agaatacaat cgcgcaaaac atcaaaaaat
     8461 ccaaaaatta agaccaaaaa aggctataaa acgcctcatc taaagaaatc tatttttaaa
     8521 cctatttgtc ttatttttat taaggataaa tatattttaa aaaaccatta aaacaaggag
     8581 tgaaaaagtc atgaaagcaa aatttcatat agttaaaaaa gtagcagata tggaacatcc
     8641 aaaaaataat tccaaaactg aaaaagacat agaagttgaa gttgatgaac aatttgaatt
     8701 agaaggcgga atttatgtat ttaaattact tgcagctaga gcaaatggtg cacaagtaga
     8761 atatgataga cattattcta ttatgaacga acacagagga tatgaattta atacaatatt
     8821 ctatatggga gagccaaaga tagttacttc tctgtggggt aagaaccaaa taacctatac
     8881 aataacctat aacggttttg gctcaagttc agaagaaaat acagaaagtt ttgaatagag
     8941 aaataattct ctattaaact tattttttat taaggaaaaa aaatgtataa acaagttata
     9001 atttttagaa atgatttaaa gcttgggaaa ggaaagattg ctggtcaagt atctcacgca
     9061 gcagtcgaag cctatattct aacttctaag aaagaccctt ctgcagttga taattggttg
     9121 atggaagggc aaaagaaagt tgtgttaaag attgatagca aaaaagaact acttgaatta
     9181 tatcaatcaa ttaaaaacaa attcccttgc gttctgataa aagacgcagc aaagactcag
     9241 ttaccagaac cagacatcac ttgtctaggc attggtccaa ttaaagaaac agagattgat
     9301 caatttacag gaaagtataa gcttttgtaa attatcaaca ctcgtattgc tttttctcaa
     9361 aatcacaaag aaatcaagca aggggtttat tattctaaaa aaagtctata ttctatgttt
     9421 agtttgctaa aggtaataaa tataaatact tagtaatatg tattatttat taattaataa
     9481 ataaatgata tatatgaata agaaaaaa
//
LOCUS       DAOOON010000024         9212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_9305_length_9212_cov_15.769357, whole genome shotgun sequence.
ACCESSION   DAOOON010000024 DAOOON010000000
VERSION     DAOOON010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_9212_cov_15.769357"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4292)
                     /locus_tag="PLK55_03465"
     CDS             complement(<1..4292)
                     /locus_tag="PLK55_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36015.1"
                     /translation="MSFALKYQGFLDKINNRIPIKLLTKPIDKIHPLASFFLFIIIII
                     VALLLIFKPFGLPGENTQTILFTIGLADDGGEILRNFEFQLKDNGNDLTQNYRTGNNG
                     QFSLELNKFGNYSVVVDKAGYKYFEADIDLSKTRQNFTVLIFNLPSTQSKILTFVGSN
                     GQPISENLNVTITCQDGTTINPTSSTVTNGTANFDVPVSCTTMVATAIGTSYSGSNIP
                     INQATGIVNVPPKAAVTGTGRATIRVKSGTNYLDGITLRIYTIEDGINPVFSGQTTMG
                     SKSFANIEIGEYKVVATDDSLRYESTESTFTITKDTETQINIDMPAVNGQGNPGDTDT
                     TIETRDITVIVKDLDTGIEFDSTDLAQVLLITDANGSLQTVDTKNYEGSSISFRTDVS
                     KEYSLTVRAPGYLAATREITPNTNSYLINLEKETVSNVSDINVTVIDEENLPVIGATL
                     IIYNGDNEFQDTRYDSLVTDESGKVSFESIAQGRYFIKLRKTNVVSSSQKFTHTPPAD
                     TNLPMTINVGTGTIRFNIKNINEETISFASIKLYNQLGEEIGTDQAAQNGEYAKTIKA
                     DKILYAKISKEGYYDYYTELIPIISGETINKDIKLIRTGTVSLLTADFVGLFNEQNQK
                     VEYLTQNSKFYFKFRLINPTAGNIGLRFLAGDKETAEEDIVYIRTMYDSKANISYYSN
                     KNLTIVTPDIHAKAIDAKFVNASVGVYEILVPVGTVQAYRGQAAPIYYSVFTTIPPIL
                     DDLINQKVYYIDTAEICSETFCLSGQYVDIEEDIVYDLAEINNLVVNKAYNLKYVLTN
                     AKSNVYNNNRLSIANVENTTEMLASAIDIISYNISGGRFNARESSGNQLRNKIPFTSE
                     YLNSLQINVYDKIDVNTNIKPAVLGTSRFQNKIISNQSVVYNEFITFNVSQQYPFTIN
                     YEPKNIVPNKPFDLTIVSLDESGLPVAGALVNIYQRRANNQQIKITSGTIRTNDYGKA
                     VIPMPSLNMGERIIINIDKSGYYAEPVEFTVEQNILSLKQDNTKITDNTPFTININKA
                     NPDGTERVLTIVNNTDYPLRLKPFKDNSFSFRYSNYLNLSKLQQSLNSQVGNIVIPAN
                     GEENLRIKIATSQDAEELTQTLNITGNIIGYVSVDNSESEYPFNIPISLKISVGEGVE
                     EDDCLVIQGANNPWKQVVSSNDSVSFSFDIFNNCKVKGNDEQSLALDTIRAKIVNSKD
                     IFGSYNLMVDGKTITLSEGSYTTILKNLAPGEHRAVLTYYSLDKKFGDVETKIYINGQ
                     VKTNDGLKYVNTTKDVAFTTNIAIRKIQDCIDFYDGSKKINDMFVITKETNAYDVKEL
                     TVKNNCADLGRFRLNFCEDIETNPNAFEGCGDIKYNNLSGFNENELSFNFGESSLVAG
                     IKKPETPGGYFIPVKVSILNSNNAPITTIYKNLKINVQSATLYMEDPFIEVE"
     gene            complement(4409..6487)
                     /locus_tag="PLK55_03470"
     CDS             complement(4409..6487)
                     /locus_tag="PLK55_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36016.1"
                     /translation="MIKNKKIQLLIIFISLIVFSSSVYSAVAISEKNYEVKSLSANQR
                     ILEEISIFKSNSAGAATEGQTPTEINLKYNTSWLENNLTKDVIFIFSKTENDSEIYQL
                     ISDFYSQNSNLLVSDYCNNLEIENTICSSIVVNQKTNIGNDGTVVFGAQTAGESSVTS
                     IPIKTEELSEYTVAYTIIYSYENFGENPENLANPEFTEVIKIKFSNNLLQHLNSENTN
                     LILSALTLEESNQTAENNYATGGLDSQINNVEKVDSQINWAIDDTYLQVNITLPESFD
                     EFFVYPYVLITEENKDTIKQSIESLFVDLVSEDTLNLDKRTGSNYSFNLEATTSGVYY
                     VLLFKKERSGRIIYTNHYLNVTLNSNLPEDYDFQIIENKNKTVSYSIGGAESITSALD
                     DTGQFYEIVSGDGALNISFNLDAEYFNKVNYIKVFDYNSENADRITNEIISAINSMYQ
                     FGRVNNFNTINSNSYSLPLDSLTKDTQKYVFILNKGADNNIYYSQFYLDIFKNKDTIY
                     NKKLLKVYKSLYYFYCSTCVELKYNLINMPNQGSEIASLISRLNANIDTDDQLIIPSI
                     VAKRDTTESETGSVVTNEYYYEIIYTNKGKACIKENYPIIQDYSVDTGVGTHFRCVCV
                     RHKGKNYYGVEKCNEYNTAVVYGPYNRIFSGADTYILGEYPSPLKESCWKAGETGCSE
                     SMTWQYPKQP"
     gene            complement(6500..6778)
                     /locus_tag="PLK55_03475"
     CDS             complement(6500..6778)
                     /locus_tag="PLK55_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36017.1"
                     /translation="MNRRQSRKKEITLKKVKDKSKHSVFWFILLIIVLFSFIFLMYNQ
                     YAAKDPNTEINRSPQYLNKDLVNRNYNPEIIVRNTPLNVNENINVDNN"
     gene            complement(6780..>9212)
                     /locus_tag="PLK55_03480"
     CDS             complement(6780..>9212)
                     /locus_tag="PLK55_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36018.1"
                     /translation="IKLDSDVDFNKLQVPRFRGDIQEKDVYLDLGNTFSNLAFIKDEE
                     TGKYRGCIKGNYSDIKDLITIKESKENLVNINPDNIRTKGLQEVNSGYVIKPNYNIEL
                     KLLRSKQYSMCLITFGPPETILSTENISDLYLTESNDPEPKIFKIYNKSKEVANIKIK
                     ININKLLGRENSSLTTKFDLEDYVEYNGEGLCFDPKGFNEDYGRTGLIEYMKYGFDKI
                     LFEWSESQINYNSCDYGENYCDQDQFRLALNKKIKELNNNNYIELEDIKFKLNDFKLI
                     ELHLARPSILDSRLNPAVDPVIDETLISEYQDDMFKINLQPIEERNSNTKEIIRRLKI
                     DLNNSVPKELQKLTIMRINFDSSKKEEYLLKTKELLNDDLNIYVIDIANKTYISFTAN
                     NFLSSTKPDKETILLEDMEYMLAFLSSLDIYYGVALNPSLVNLKPFGDLTGETNNILT
                     EIYFLSNREDYTYNIDATSEIKNIDKPNVYLLYLENIDDSKKTANYKLSKLSDISESK
                     INIYENRLEYKDNLFFTININARYNDYGETPFDLSITQHAKTKSSNIKVYSDYSDLLN
                     IQDGYIYELKTTQNGQIQDSQFADIIPLQITSKDITGNAFSIDVYDDSVRGDYKKSNI
                     ALRSLIPTSDSTPQIITTHLSSDHTYNFTTPIKTSVFVFNNFKDSGNKPQMLIESEKE
                     LYFNLLDTDSRKEIRNNKILYSLPSKLETLGKYQAQINTSGFNYMIYGATDNPLVGGI
                     RYPSILNNHICFRFENTNNEKNFYLWSNPAILSGKTNNNPNIFVIKTESGEYYIDFSA
                     GQGGYYIAEKKD"
BASE COUNT         2826 a         1390 c         1270 g         3726 t
ORIGIN      
        1 ttttctactt caataaatgg atcttccatg taaagggttg ctgattgtac gtttattttt
       61 aagttcttgt agatggtggt tattggtgca ttattgctgt ttaagattga aacttttact
      121 ggtataaaat accctccagg agtttctggc tttttaatac ctgcaacaag agagctttct
      181 ccaaaattaa aacttaattc gttttcatta aagccactaa gattgttgta ttttatatct
      241 ccacaacctt caaaggcatt tggatttgtt tcaatatctt cacagaaatt taatctaaat
      301 cttccaagat ctgcacagtt gttctttact gtgagttctt ttacatcata agcatttgtt
      361 tctttagtaa taacaaacat gtcgtttatc ttcttactac catcatagaa atcaatacag
      421 tcttgtatct ttcttattgc tatgtttgtt gtaaatgcta cgtcttttgt ggtatttaca
      481 tattttagac cgtcgttagt cttaacttgg ccattaatgt aaatctttgt ttctacatct
      541 ccaaatttct tatcaagaga ataataggta agaactgcac gatgttctcc tggagctaaa
      601 ttttttaata ttgttgtgta actgccttct gaaagagtta tggtttttcc atcaaccatt
      661 aaattataac taccaaaaat atctttagaa ttaacaatct ttgctcttat tgtgtcaaga
      721 gctaaacttt gttcgtcatt tcctttgact ttacaattat taaatatatc aaagctaaaa
      781 gacacagaat cattactgct tacaacttgc ttccaaggat tatttgcgcc ttgtattact
      841 aaacaatcat cttcttctac gccttcgcca acagagatct ttaggcttat tggaatatta
      901 aatggatatt ctgattctga gttgtctaca gaaacataac ctataatgtt accagtaata
      961 tttaaagttt gagttagctc ttctgcatcc tgagaagttg caatctttat tcttagattt
     1021 tcttcaccgt ttgctggtat tacaatatta ccaacttggc tatttagaga ttgctgtaat
     1081 tttgaaagat ttaggtaatt agaatatcta aagctaaagc tattgtcttt aaatggcttt
     1141 aatcttaacg gataatctgt gttatttact attgttaaaa ccctttctgt accgtcagga
     1201 tttgccttgt taatgttaat tgtaaatggc gtgttgtctg ttatttttgt attatcctgt
     1261 tttaaagaaa gaatattttg ttctaccgta aattctactg gctcagcata atatccgctt
     1321 ttatcaatat taataattat tctttcgccc atattaagag atggcatagg tattactgct
     1381 ttaccataat cgtttgttct gattgttcca cttgttatct ttatctgttg attatttgct
     1441 cttctttgat aaatattaac aagagcgcca gctactggta agccggactc atctaaagaa
     1501 acaattgtaa gatcaaatgg cttatttgga acgatatttt ttggctcata attaatagta
     1561 aaaggatatt gctgagatac attaaaagtt ataaactcat tataaacaac agattgatta
     1621 gaaataatct tgttctggaa tctggaagtt ccaagaactg ctggcttaat gtttgtgtta
     1681 acatcaattt tatcataaac atttatttgt aaagaattta gatattctga cgtaaatgga
     1741 atcttgtttc ttaactggtt tcctgaagac tccctagcat taaatctgcc accagaaata
     1801 ttatagctaa taatatcaat tgcactagca agcatttctg ttgtattttc aacatttgct
     1861 attgatagtc tgttgttatt gtaaacattt gattttgcat tagttaaaac atactttaga
     1921 ttatatgctt tatttacaac tagattatta atttctgcta aatcataaac aatatcttcc
     1981 tcaatatcta catactggcc agataaacaa aaagtctctg aacagatttc tgcagtatca
     2041 atataatata ctttttgatt tattaaatcg tctaatattg gtggaatagt tgtaaacaca
     2101 gaataataaa ttggtgctgc ttggcccctg tatgcttgaa ctgttccaac tggaactaat
     2161 atttcataaa caccaacaga agcatttaca aactttgcat caattgcttt tgcgtgaatg
     2221 tctggtgtca ctattgttaa atttttatta ctataataac taatatttgc ctttgaatca
     2281 tacattgttc ttatatatac tatatcctct tctgcggttt ctttatctcc tgctaagaac
     2341 ctcaagccaa tatttcctgc tgttggattg attagtctaa acttaaaata gaactttgaa
     2401 ttttgagtta aatactctac tttttgattt tgctcattaa acagaccaac aaagtctgct
     2461 gtaagaagac taactgttcc ggttcttatt aattttatgt ctttgttgat tgtttctcca
     2521 ctaattattg gtattaattc tgtataataa tcataatatc cttcttttga aatctttgca
     2581 taaagtatct tgtctgcttt tatagtttta gcatactctc cgttctgtgc tgcttggtct
     2641 gttccaatct cttcgcctaa ttgattatat aatttaatgc ttgcaaagct tattgtttct
     2701 tcatttatgt ttttaatatt aaatcttata gttccagttc caacatttat tgtcattggc
     2761 agatttgtat ctgctggggg tgtgtgagtg aatttttgag aagaagaaac tacatttgtt
     2821 tttcttagtt ttatgaaata tctgccttga gctatacttt caaaactgac tttcccagat
     2881 tcatcagtaa ctaaagaatc atatcttgtg tcctggaatt cgttgtctcc attatagata
     2941 attaaagttg caccaattac tggaaggttt tcttcatcaa tgactgtaac attaatatct
     3001 gaaacattac ttactgtttc tttttctaaa tttataagat agctgtttgt atttggagta
     3061 atctctctag tggctgcaag atagcctggg gctctaactg tcaatgaata ttcttttgaa
     3121 acatctgttc taaatgaaat actagaacct tcataatttt tagtatcaac tgtttgtaaa
     3181 ctgccattag catcagttat caataatact tgtgctaaat cagtagaatc aaattctata
     3241 ccagtatcta gatctttaac aattacagtt atgtctcttg tttctattgt tgtatcggta
     3301 tctcccggat ttccttgccc attaactgct ggcatatcaa tatttatttg tgtttctgtg
     3361 tcttttgtta tagtaaaagt gctttctgtg gattcatatc ttaaggaatc gtctgttgca
     3421 acaactttgt attctccaat ctcaatatta gcaaaagatt ttgaacccat tgttgtttga
     3481 ccagaaaaaa caggattaat tccgtcttct attgtatata tccttaaagt aattccatca
     3541 agataatttg tgccactttt tactcttatt gttgctctac ctgttcctgt tactgcggct
     3601 tttggtggaa catttacaat gcctgttgcc tgatttattg gaatattgct accagaataa
     3661 cttgtaccaa ttgctgttgc aaccatagtt gtacaagata ctggaacatc aaagttagct
     3721 gtcccgtttg ttactgttga agaggttgga ttaattgttg ttccatcttg gcaagtaatt
     3781 gttacattta gattttcaga tattggttgg ccatttgatc ctacaaaagt taatatcttt
     3841 gattgcgtac ttggtaaatt aaaaatcaat actgtaaaat tctgtctagt cttacttaaa
     3901 tcaatatctg cttcaaaata tttatatcct gctttatcaa caacaacaga ataatttcca
     3961 aacttattta gttctaaaga aaattgacca ttattcccgg ttctataatt ttgagtaagg
     4021 tcattgccat tatcttttaa ttgaaactca aaatttctta agatttctcc accgtcgtct
     4081 gcaagtccga ttgtaaatag tatagtttgt gtgttttctc ctggtagccc aaatggttta
     4141 aatataagca ataaagcaac tattataata attataaaca aaaaaaaaga agctaatgga
     4201 tgtattttgt caattggttt tgttaacaat ttgattggta ttctgttgtt tattttatct
     4261 aaaaagcctt ggtattttaa agcaaatgac attatctttc acatccgtaa atatatatct
     4321 aaaatatatt tataattaaa ggaaataagg tatttaaatg tatctgtgag gttaaaaagt
     4381 taataaaaaa agggaaagga gaaaatattt aaggctgctt aggatattgc caggtcatac
     4441 tttctgaaca cccggtttct cctgctttcc aacaggattc ctttaatggg cttggatatt
     4501 ctcctaaaat ataagtatct gcaccactaa atattctgtt gtaaggccca taaacaacag
     4561 cggtgttata ttcattacat ttttctacac cgtaataatt ctttccttta tgccttacac
     4621 aaacacatct aaagtgtgtt ccaacacccg tatcaacact ataatcttgt attattggat
     4681 agttttcttt tatacaggct tttcctttat ttgtgtagat tatttcataa taatattcat
     4741 ttgtgaccac agacccagtt tcactttctg ttgtatccct ttttgctacg atacttggga
     4801 tgattaattg atcatctgta tcaatatttg catttaatct tgatattaat gatgcaatct
     4861 cggaaccttg attaggcata ttaattaaat tatattttaa ttcaacacaa gtactacaat
     4921 aaaaataata tagtgattta taaactttta ataatttttt attataaatg gtgtctttgt
     4981 ttttgaatat atctaagtaa aactgagaat aataaatatt attatctgcg cctttattta
     5041 aaataaaaac gtatttttga gtgtcttttg ttaggctatc taaaggcaaa ctatagctgt
     5101 ttgaattaat tgtgttaaaa ttattgactc ttccaaattg atacatagag ttgattgccg
     5161 aaattatttc gttagttatt ctgtctgcat tttctgaatt ataatcaaat acttttatat
     5221 aatttacctt attaaaatac tctgcgtcta aattaaaaga aatattaagt gcaccatctc
     5281 cacttacaat ttcataaaat tgtcctgtat cgtctagagc ggatgtaatg gattctgctc
     5341 ccccgataga ataggaaaca gttttatttt tgttttctat aatttggaaa tcataatctt
     5401 cgggcaaatt agaatttaaa gttacattca aataatgatt agtataaatt attcttccac
     5461 ttctttcttt tttaaataat aacacataat aaactcctga tgttgttgcc tctaaattaa
     5521 aagaataatt tgagccggtt cttttatcaa gatttagggt gtcttcagaa actaaatcta
     5581 caaacaaact ttctattgat tgttttattg tatctttatt ctcttcagta attaaaacat
     5641 aaggataaac aaaaaactcg tcaaaacttt caggaagtgt aatattaact tgtaaatagg
     5701 tatcatctat agcccaattt atttgactat ctactttttc aacattatta atttgagaat
     5761 ctaatccccc agtagcataa ttattttctg cagtttgatt tgattcttct aaagtcaatg
     5821 ccgacagtat gagatttgta ttttctgaat ttagatgttg caaaaggtta ttgctaaatt
     5881 taattttaat aacttctgta aattcaggat ttgctaaatt ttcagggttt tctccaaaat
     5941 tttcataaga ataaattata gtatatgcga cagtatattc tgaaagctct tctgttttaa
     6001 tgggaattga tgtgactgat gattctccgg cggtttgtgc tccaaaaaca acagtgccat
     6061 catttccaat atttgttttt tgattaacta caatagaaga acaaatagta ttttctattt
     6121 ctaaattgtt acaataatct gaaactaata gattgctatt ttgagaataa aaatcagata
     6181 ttaattgata aatttctgaa tcattttcag ttttagaaaa tataaaaata acatcttttg
     6241 ttaaattatt ttctaaccaa ctagtattgt attttagatt aatttctgtt ggcgtttgtc
     6301 cttctgttgc agcgcctgca gaattagatt taaaaataga tatttcttct aatattctct
     6361 gatttgctga cagagatttg acttcataat ttttttctga aattgccacg gctgaataaa
     6421 cactagaaga aaacacaatt aaagatataa aaattattaa caactgaatt tttttatttt
     6481 ttatcatatt ttacaccggt taattattat ctacattaat attttcatta acatttaaag
     6541 gagtgtttcg cactattatt tctggattat agtttctatt tactaagtct ttatttagat
     6601 attgcggact tctattaatt tctgtattag ggtcttttgc agcatattga ttatacatta
     6661 agaaaataaa cgaaaataac acaataatta ataaaataaa ccaaaaaaca gaatgttttg
     6721 atttgtcttt tacttttttt agtgttattt cttttttcct actttgtctt ctattcatat
     6781 taatccttct tttcagcaat ataatagccg ccttgtccag cactaaaatc tatataatat
     6841 tctccagatt cggtttttat aacaaaaata tttggattat tatttgtttt tcctgataat
     6901 attgctggat tagaccacaa ataaaaattc ttttcgttgt ttgtattttc aaatctgaaa
     6961 caaatgtgat tattcaatat tgagggatat cttatgccac caactaatgg attgtctgtt
     7021 gctccataaa tcatataatt aaatccgctg gtgtttattt gtgcttgata tttccctagc
     7081 gtttctagtt ttgaaggcaa agaatacaat attttattat tccttatttc ttttcgtgaa
     7141 tctgtatcta ataaattaaa atataattct ttttcagatt ctataagcat ctgtggtttg
     7201 ttgccgctgt ctttaaagtt attaaataca aacacactag tttttattgg tgttgtaaaa
     7261 ttataggtat gatcagacga tagatgggtt gtaattattt gcggagtaga atcgcttgtt
     7321 ggaataagac ttcttaaagc aatatttgat tttttatagt ctcctcttac agagtcatca
     7381 tatacatcta ttgaaaatgc atttccggtt atgtctttag aagttatttg aagtggaatg
     7441 atgtctgcaa attgggagtc ttgtatttgc ccgttttgag ttgtctttaa ttcatagata
     7501 tatccgtctt gaatatttaa taaatcggaa taatcagaat atacttttat gttgcttgat
     7561 tttgttttag cgtgttgagt aatacttaaa tcaaatggag tctctccata atcattatat
     7621 cttgcattaa tgttgattgt aaaaaataaa ttatctttat attctaggcg attttcgtaa
     7681 atgtttattt ttgattcaga gatatcagat aattttgata atttataatt tgctgtcttt
     7741 ttagaatcat caatattttc tagatataat aaataaacat ttggtttatc tatgttcttt
     7801 atttctgaag tggcgtctat attataagta taatcttctc tgttacttaa gaagtatatt
     7861 tctgttagta tgttatttgt ttctcctgtt aaatcaccaa atggtttaag atttacaagc
     7921 gatggattta aagcaactcc gtagtatata tcgagactac ttaaaaatgc taacatatat
     7981 tccatatctt caagtaagat tgtttcttta tctggttttg tagaacttaa aaaattgttt
     8041 gctgtaaaag atatataggt tttattcgct atatctatta catagatatt taaatcgtca
     8101 tttagcagtt cttttgtttt aagtaaatac tcttctttct tagaactatc aaaattaatt
     8161 ctcatgattg ttaacttttg taattctttt ggtacagaat tatttaaatc tatctttaat
     8221 ctgcgtatta tttctttagt attgctattt ctttcttcta ttggttgtaa atttatttta
     8281 aacatatcgt cttgatattc tgaaattaat gtttcatcaa ttactgggtc taccgctgga
     8341 tttaatctag agtctagtat tgagggcctt gctaaatgta gttctataag tttaaaatcg
     8401 tttaatttga atttaatgtc ttctaattct atatagttat tattatttag ttcttttatc
     8461 tttttattta gtgctaatct aaattggtct tgatcacaat agttctcacc atagtcgcag
     8521 gaattatagt tgatctgaga ttctgaccat tcgaatagta tcttatcaaa tccatatttc
     8581 atatattcta ttaaaccggt cctaccataa tcttcattaa atcctttagg atcaaaacat
     8641 aggccttcgc cgttgtattc tacgtaatct tctaaatcaa actttgtagt tagtgatgaa
     8701 ttctctcttc ctaataactt attaatattt atttttattt tgatatttgc aacttcttta
     8761 gatttattat atatcttaaa tatctttggt tcagggtcat tactttctgt taaataaagg
     8821 tcagaaatgt tttcagtaga tagtattgtt tctggaggtc caaaggttat caaacacatc
     8881 gaatattgct tagatcttaa tagttttaat tcaatattat aatttggttt tataacataa
     8941 ccagaattaa cttcttgtaa tccttttgtt cttatattgt ctggattaat attaactaaa
     9001 ttctctttgc tttcttttat agtaattaaa tcctttatgt ctgaataatt acctttaata
     9061 caccctctgt attttccggt ttcttcatct ttaataaatg ctaaattgct aaaggtattc
     9121 cctaaatcaa gataaacgtc cttctcttga atatcccctc taaatcttgg aacttgtaat
     9181 ttattgaaat ctacatcaga atccaatttt at
//
LOCUS       DAOOON010000025         8932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_9663_length_8932_cov_16.105554, whole genome shotgun sequence.
ACCESSION   DAOOON010000025 DAOOON010000000
VERSION     DAOOON010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9663_length_8932_cov_16.105554"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PLK55_03485"
     CDS             complement(<1..1394)
                     /locus_tag="PLK55_03485"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HOZ36019.1"
                     /translation="MNSRDLYKTLNEKQKTYVNLKMTDSQLHNGEYMLTVFHFKPGKN
                     LNTLQAAAEIAAESSSGTNFPVTTETPFSRQMNALVYKMDIKNELVWIAYPWRLFDRR
                     GNVQNILTYVVGNVLGMKEVSALKLLDMWFPPSMLEQYDGPSYTIDDMRKYLGVYDRP
                     ILGTIIKPKIGLTSAEYAEVCYDFWVGGGDFVKNDEPQADQDFCPYDKMVKHVKEAMD
                     KAVKKTGHKKVHSFNVSASDFDTMIKRCELIRSAGFEKGSYAFLIDGITAGWMAVQTL
                     RRKYPDVFIHFHRASHGAYTRPENVFGFSVLVLSKFARLAGASGIHTGTAGVGKMAGT
                     PEEDVVAAHNILNFTAKGHFFEQSWSTIPQKDIDIIKLNKKDKNKKRILEDDSWRGLK
                     KCAPIISGGLNPTLLKPFIEIMGGVDFITTMGAGVHAHPDGTEAGARALVQACEAYKK
                     KIDIHKYAKDPKHK"
     gene            1471..2367
                     /locus_tag="PLK55_03490"
     CDS             1471..2367
                     /locus_tag="PLK55_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOZ36020.1"
                     /translation="MRTKILSFIYSRKNNNWLLLKNNGADPIHGGEKWFTVTGSVESE
                     DKSLEDAVARELLEEINQAPIKIINLNTKSEYYSEYDKEDCLEYNFISVVNSDKVTLD
                     TIENTDYLWVDLEEYIEKIWWLEDKNKLKEQLENEIKNLNITAENIDNTYLPYGSYVE
                     VYILNENKEFIVVFSALKGNFCKVLGGGVEKGESFEEAAIREAKEELNADIKIIGVSK
                     HPSDFEISKKSLFNNYFKSRGGRGKIIIAKLDPPYQRLKIQADEISGYKWINKDQIYN
                     NLEILDQWISAEKIFKEFADYF"
     gene            complement(2375..3097)
                     /locus_tag="PLK55_03495"
     CDS             complement(2375..3097)
                     /locus_tag="PLK55_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36021.1"
                     /translation="MAAAARMKPFRIIKKGTKSVVRSRGGNKILSQADIITALSELSK
                     KQTLTNIKDPQGRFAISKQMSFKSDGEHIQRNYVLDIKDKNGKIKKYFIKEEKLVPGL
                     EKESIIPSYTWEDSITEAKALNVVSDYLKQHASANIATVTLKFAFQDKEKNTSVICYN
                     YQYGHHPKTLLQKGQIKIKEYTDISRRLRILEDKVNDYLKQNSERYGLSPETRITDID
                     VDNAFYDPKTKKIRLFNPILIK"
     gene            complement(3104..3508)
                     /locus_tag="PLK55_03500"
     CDS             complement(3104..3508)
                     /locus_tag="PLK55_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36022.1"
                     /translation="MLKLRTARRTNIKSPQKIKQERLPKEIALKLENIERANSKARKP
                     VTTIYRLHSNIKELITEINYQLNSKSKNSSNRLNEKLFQAIEIADIVNSYRDPIVYKY
                     LDSAEKAGLRIMEKDYKELFKLIKEVHIKTQK"
     gene            complement(3573..4130)
                     /locus_tag="PLK55_03505"
     CDS             complement(3573..4130)
                     /locus_tag="PLK55_03505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOZ36023.1"
                     /translation="MKIYFITGNKNKLEEIKSLFSDIPEIEIENLKLDLPEIQELDSK
                     KVIFEKIEVAKLDKSLKNIFVEDTSLCFDYFNGFPGPLIKWLYTTAGPQKISEMLKPN
                     NKATAIVSIGFYDGKDTYFFEGKVTGKIVSPRGDDRFAWDLIFMPDGYDKTYAEMPIE
                     EKNKISMRRLAILKFKEYLVSQLKG"
     gene            complement(4142..4888)
                     /locus_tag="PLK55_03510"
     CDS             complement(4142..4888)
                     /locus_tag="PLK55_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOZ36024.1"
                     /translation="MNTIGILWLRQIKRQYRSKSRMIGSLAMPLLFLLAFGFGFGPVF
                     AQAGQGNYLQFLVPGMISMSILFASVMSGIEIIWDRQFGFLKETLVAPVPRLEIMIGR
                     TLGGATVAFAQGLLFLFLSMLFGFRIISWTLLPIAILFMILIALFFAALGTAIASLLN
                     DMQGFQLIMNFLIMPIFFLSGALFPLEGVPKAIKVLSTINPLSYGVSGLRSALAGTAT
                     GNMFFNLILLGVLAIIMLVIGSKLFSKMQV"
     gene            complement(4904..>5329)
                     /locus_tag="PLK55_03515"
     CDS             complement(4904..>5329)
                     /locus_tag="PLK55_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOZ36025.1"
                     /translation="ELWDRKNELVKNFSGGMKRRLEIARGLLHYPKVLFLDEPTVGLD
                     PQTRNHIWDYIKKVNKREGTTIFFTTHYMDEAERYADSIAIIDYGKIIDIGTSKELIK
                     KTKTNTLEEAFIKITGYSIRDEEVSTIDRMRQNRKMHHK"
     assembly_gap    5330..5429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5651..6133
                     /locus_tag="PLK55_03520"
     CDS             5651..6133
                     /locus_tag="PLK55_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOZ36026.1"
                     /translation="MKNYLKLILAMLFCLGAGVIGSFATETGPGSWYQAINKPPITPP
                     GVVFPIAWTTLYILMGLALYIIWSQPQKYRKDPELKRSAMTLFALQLILNLSWSFLFF
                     SLQSPIAGLICIFALVGLVAITTIKFYKLNEKAAYLMIPYLLWLCFATVLNLMILILN
                     "
     gene            complement(6169..6870)
                     /locus_tag="PLK55_03525"
     CDS             complement(6169..6870)
                     /locus_tag="PLK55_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36027.1"
                     /translation="MPSLKPKISGKRIILKKPIERKIQRSLPKIPEKMAVKIYERRYA
                     VSAKDIQRKLDLFNRTLKESKKYSSYEEMYKDILKDHKKQPSAETKEFIRTVEDLRTD
                     RLLDLKNGRKKMNRLLEIYSHRELLNTEFLLYAGYKPIELATSLAVYDMTMRKAGLER
                     YIGDSETSQRTRNPKLNNRNPKSFIEAPGYEGFVKNHINWDKVIESLRNGTFRKTDFK
                     VFFSKESLSLINNNI"
     gene            complement(6917..7471)
                     /locus_tag="PLK55_03530"
     CDS             complement(6917..7471)
                     /locus_tag="PLK55_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOZ36028.1"
                     /translation="MSKDHYEIEVKVLEVNKKELENKLKEIGAKKRFSDKKVIQENYD
                     NKELKKKNAAIRVRQLGKDVFITFKQFKEIRNNIKSCKEIEFQVSSDFKTVQDMFKSL
                     GLKRYHRIEKTRTTYLLGTTTFDIDTISKPFRIPTYYEIEVKEYKDLDKILKLLGIPK
                     NKVKPWGTKRLLKYYGSKSNNNNL"
     gene            complement(7481..7999)
                     /locus_tag="PLK55_03535"
     CDS             complement(7481..7999)
                     /locus_tag="PLK55_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOZ36029.1"
                     /translation="MSKDHYEIEVKVLEVNKKELEKKLKEIGAKKKAKRKIKTILYGV
                     PGKENKNVIIRLRKKPGVFSITYKKVLKTNKPVKIAEEIGVKVSDYNKMKLILDKLFI
                     KKLEYEKEGILYIKDGTIFGIDTIKGIPTFLEIEAKSIKVIEKYIKILKIDKKKIRKW
                     TAEQLFKNYKLI"
     gene            complement(7996..>8932)
                     /locus_tag="PLK55_03540"
     CDS             complement(7996..>8932)
                     /locus_tag="PLK55_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOZ36030.1"
                     /translation="MKYTKDLLWTKGMSVSDLVDSYSSTGFQSSELSRAANLIEKMKK
                     EGAKIYLSFTSNMVTSGLRGFFSQVLKLKMASVVCTTVGAIEEDIMKADGEKFILASF
                     KADDQELYERGENRVGNILITNESYAKLEGKMQKLFLELYKQKKVWSTCELLREIGLK
                     LNDENSFLYQAAKNNIPVFCPAITDGSFGFHLYLFQQDHKDFMIDIISDFKQIIFSTT
                     HDEKKGIIALGGGVSKHHVILTTLINGGADYALYMTTASSRSGSLSGATTDEAKSWGK
                     IKDEADAVTVVGDVTITFPLAMIKALENLSKEGII"
BASE COUNT         2640 a         1521 c         1222 g         3449 t
ORIGIN      
        1 tctttgtgct ttgggtcttt agcatacttg tgaatatcta tctttttctt gtatgcctca
       61 caagcttgga ctagagccct tgctcctgct tctgtgccat caggatgagc gtgaactcct
      121 gcgcccatgg ttgttataaa atcaactcct cccataattt ctataaatgg ttttaataga
      181 gttggattta aaccaccaga aattattgga gcacatttct ttagacctct ccaggagtcg
      241 tcttctaata ttcttttctt gtttttgtct ttcttattta attttataat atctatatcc
      301 ttctgtggta ttgttgacca agactgctca aagaaatgtc cttttgctgt aaaatttaaa
      361 atattatgtg cagcaacaac atcttcttct ggcgttcctg ccatctttcc aacacctgca
      421 gttcctgtat gtattccgct tgcccctgca agtcttgcaa acttagaaag cactaatact
      481 gaaaaaccaa agacattttc tggtctagta tatgcaccat gtgatgccct atggaagtga
      541 ataaacacat cagggtattt tcttcttaga gtctgaactg ccatccaacc tgcagttatt
      601 ccatctatta agaatgcata agaacctttt tcaaagcctg cgcttcttat tagctcacat
      661 ctttttatca tggtgtcaaa gtcagaagca gagacattaa aagaatggac cttcttatgg
      721 cctgtttttt taactgcttt atccattgct tctttaacgt gttttaccat cttgtcgtaa
      781 gggcagaaat cctggtcagc ctgtggctca tcattcttaa caaagtctcc tccgcctacc
      841 cagaagtcat aacacacttc tgcatattct gctgaggtta agccaatctt tggtttaata
      901 attgttccaa gaattggtct gtcataaact cccagatatt ttctcatgtc atcaattgtg
      961 tagcttgggc catcatattg ttcaagcata cttggtggaa accacatgtc taatagcttt
     1021 aaagcagaaa cttctttcat gcctagaaca ttacccacaa cataagttag aatattttga
     1081 acgtttcctc ttctatcaaa tagtctccaa ggatatgcaa tccatactag ttcgttttta
     1141 atatccatct tatagactaa agcattcatc tgtctagaaa atggagtttc tgttgttact
     1201 ggaaaattag ttccagaaga agattctgca gcaatctctg ctgctgcttg aagagtgttt
     1261 agattttttc ctggtttaaa atggaaaaca gttagcatat actctccatt atgtaattgg
     1321 ctatctgtca ttttaagatt gacataagtc ttttgttttt catttaaagt tttatataaa
     1381 tctcttgaat tcataaacat ctccctccct aaatataata ataaaataga ttataaacta
     1441 gtttaaaaag attgtggttt taaaatattt atgagaacga agattctttc ttttatatat
     1501 tcaagaaaaa ataataactg gcttcttcta aagaataatg gtgcagaccc aatacatggc
     1561 ggagagaaat ggtttactgt tactgggtct gttgaatctg aagacaagtc tttggaagac
     1621 gccgtagcca gagaattact tgaagaaata aatcaagcac caataaagat aattaatcta
     1681 aacactaaaa gtgaatacta cagcgaatat gataaagaag actgtttaga atataatttc
     1741 atttctgtgg ttaactctga taaagtaaca ctagacacta tagaaaacac agattatctt
     1801 tgggtagatc ttgaggagta cattgaaaag atttggtggt tagaggataa aaacaaatta
     1861 aaagaacaat tagaaaatga aataaaaaac ctaaatatta ctgctgaaaa catagacaat
     1921 acttatctcc cttatggttc ttatgttgaa gtatatattc taaatgaaaa taaagaattc
     1981 attgttgtct tttctgcttt aaaaggaaat ttctgtaaag tacttggtgg aggagttgaa
     2041 aaaggagaaa gctttgaaga agccgcaata agagaagcaa aggaagaatt aaatgcggat
     2101 attaaaatta ttggtgtatc gaaacatcct tctgattttg aaatatcaaa aaaaagttta
     2161 ttcaataatt attttaaatc tcgtggcgga agaggaaaaa taattattgc taaactagat
     2221 cctccttatc agcgtcttaa gatacaagca gacgagatat ctggatataa atggataaat
     2281 aaagatcaaa tatataataa ccttgaaata cttgatcaat ggatatctgc agaaaagatt
     2341 tttaaggaat ttgcggatta tttttgattt taaatcattt aattaaaatt ggattaaata
     2401 gtcttatctt ttttgtcttt ggatcataaa atgcattatc tacatctatg tctgtgattc
     2461 tggtttctgg gcttaagccg tatctttctg aattttgctt taaatagtcg tttactttgt
     2521 cttctaatat tcttagtctt ctactaatat ctgtatattc tttaatcttt atctggcctt
     2581 tctgtaaaag ggtctttgga tggtgcccat attggtaatt atagcatata acactggtgt
     2641 tcttctcttt atcctgaaat gcaaacttaa gagttactgt tgctatattt gctgaagcgt
     2701 gttgttttaa ataatcagaa actacgttta atgccttggc ttcagttatt gaatcttccc
     2761 aagtataaga aggaataatg ctttcttttt ctaatcctgg cactaatttt tcttctttta
     2821 tgaaatattt ctttattttt ccgtttttat ctttaatatc tagaacataa tttctttgaa
     2881 tatgctctcc atcagactta aagctcattt gcttagagat tgcaaatcta ccttgtggat
     2941 ctttaatatt tgttaaagtt tgttttttag aaagctcaga cagcgctgta attatgtctg
     3001 cttgacttaa aattttattt ccgcctcttg aacgaaccac agatttagtt ccttttttta
     3061 taattctaaa aggtttcatt cttgctgcag ctgccattat ttatcacttt tgtgttttaa
     3121 tgtgcacttc tttaattaat ttaaataatt ctttataatc tttttccata attcttaaac
     3181 cagctttttc tgcagaatct aaatacttat aaactattgg atctctataa gaattcacta
     3241 tatctgcaat ttctatagcc tgaaataatt tttcatttaa tctattagaa ctatttttgg
     3301 atttagaatt taattgataa tttatttctg taattaattc ttttatattg ctatgaagtc
     3361 tataaatagt agttactggt tttctagctt ttgaatttgc tctttctata ttttctaatt
     3421 ttaatgctat ttcttttggc aatctttcct gtttaatttt ttgtggagat ttaatattcg
     3481 ttctccttgc agttcttaat tttagcatag tttactcctt aaaagtaaaa tatgtatata
     3541 actatatgat ttataagcca ttatgttttt tgttatcctt tcaattgact aactaaatat
     3601 tctttaaact taagaattgc aagacgcctc atgcttattt tgtttttttc ttctataggc
     3661 atctctgcat aagtcttatc gtaaccatct ggcataaata tcaaatccca agcaaaccta
     3721 tcgtctcctc ttggagaaac aatctttcct gttacctttc cttcaaagaa ataagtgtct
     3781 tttccatcat aaaaaccaat agaaactatt gctgttgcct tgttatttgg ttttaacatc
     3841 tcacttatct tttgtgggcc tgctgttgtg tatagccact tgattaaagg gccaggaaaa
     3901 ccattaaaat aatcaaagca aagagaagtg tcttcaacaa atatgttttt taaggacttg
     3961 tcaagtttag caacttctat cttttcaaaa attacttttt tagaatcaag ttcttgtatt
     4021 tctggaagat caagttttaa gttttcaatc tcaatttctg gaatatcaga gaaaagactt
     4081 ttgatttcct caagtttgtt tttatttcct gtaataaaat atattttcat ttttttgccc
     4141 cctatacttg catctttgag aaaagcttag aacctatgac aagcattatt attgctaaaa
     4201 ctccaagaag tattagatta aagaacatat taccagttgc tgttcctgct agtgccgacc
     4261 taagcccaga aaccccgtaa cttaaaggat taatagttga taaaaccttg attgctttag
     4321 gaacgccttc taaaggaaag agcgcgccag ataaaaagaa gattggcatg attaagaaat
     4381 tcatgattag ctgaaagccc tgcatatcat taagaagact agcaattgct gttccaagag
     4441 ctgcaaagaa aagggcaatt aatatcataa acaaaattgc gattggcaat agtgtccaag
     4501 aaataatgcg aaatccaaaa agcattgata aaaatagaaa taaaagacct tgagcaaaag
     4561 caactgtcgc tccgccaagt gttcttccaa tcattatctc taaacgtgga actggtgcta
     4621 caagtgtttc ttttaagaaa ccaaattgcc tatcccagat tatctctatt cctgacatca
     4681 ctgacgcaaa caatattgac attgaaatca ttcctggaac aagaaactgt aaataatttc
     4741 cttgccctgc ttgagcaaat actggaccaa atccaaaacc aaatgctaaa agaaacaata
     4801 aaggcatagc aagagaacca atcattcttg attttgatct atattgcctt ttaatctgtc
     4861 ttaaccataa tattccaatt gtgttcatgt cttttcttcc tttttatttg tgatgcatct
     4921 tcctattctg cctcattctg tctattgtgc taacttcttc gtctcttata ctatatcctg
     4981 tgattttaat aaatgcttct tctaaagtat ttgtttttgt tttcttaatc aattctttac
     5041 ttgttccaat atctattatc tttccgtaat ctattattgc aatactgtct gcataccttt
     5101 ctgcttcatc catataatgt gttgtaaaga atattgttgt gccttctctt ttgtttactt
     5161 tcttaatata atcccaaatg tggtttctag tctgggggtc aagaccaact gttggctcat
     5221 caaggaacaa aacttttgga taatgtaata aacctcttgc aatttctagt cttcttttca
     5281 tcccaccaga gaagttctta acaagttcgt tttttctatc ccaaagctcn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttggtccaag aaaagcaaat atactttttc
     5461 ttttaacaga aaaactcacg tcttttaaaa cttctagctt tccatagctt ttgtttagta
     5521 aatttacttc tataatctta tctttattca ttattatcaa ctttattatt agaatttgaa
     5581 tataatatat ataatctata tatttaaatt gcttttgttg ctataaatag tatattaata
     5641 tgtgacattt atgaaaaact atttaaaatt aattttagca atgctatttt gtctaggcgc
     5701 aggagtaatt ggctcttttg caacagaaac tggaccaggg tcttggtatc aggcaatcaa
     5761 taagccacca atcactccac caggcgttgt ttttccaata gcctggacaa cactttacat
     5821 attaatgggt ttagcacttt atatcatctg gtcacaacca cagaaatata gaaaagatcc
     5881 agagcttaaa agatcagcaa tgactttatt tgctttacaa ttgatactta atctttcttg
     5941 gtcattttta ttctttagct tacaatcacc aattgctggg ttaatctgta tctttgcttt
     6001 agtaggtctt gttgcaataa caactataaa gttctataag ctaaacgaaa aagcagcata
     6061 tctaatgata ccatatcttc tatggctttg ctttgcaaca gtccttaatc taatgatttt
     6121 aattttaaac tagattttta tgttttttaa tccattctaa tttttttatt agatattatt
     6181 atttattaaa gataatgatt ctttactaaa aaacacttta aaatctgttt ttctaaatgt
     6241 gccatttctt aaagattcaa ttactttatc ccagtttata tgatttttaa caaacccttc
     6301 ataaccaggt gcttctataa aactctttgg atttctattg ttcaattttg ggtttcgggt
     6361 tctttgactg gtctctgaat ctccaatata tctctctaac cctgcttttc tcatagtcat
     6421 atcataaact gctaaacttg ttgctaattc aattggttta taacctgcat ataataaaaa
     6481 ttctgtattt aataattctc tgtggctata gatttctaat aatctgttca ttttctttct
     6541 gccgtttttt aaatctaata gtctatctgt tcttaaatcc tcaacagttc ttataaattc
     6601 ttttgtttct gcacttggtt gttttttatg atcttttaaa atgtctttat acatttcttc
     6661 ataagaacta tacttcttcg attcttttaa tgttctatta aataaatcta atttcctttg
     6721 tatatcttta gcagaaacag catatcttct ctcatatatt tttactgcca tcttttctgg
     6781 aatctttggt aatgatcttt gtatttttct ttcgattggt tttttaagaa taattctttt
     6841 tccagatatt tttggtttta gacttggcat aacacatcac attttaatta tataaaaact
     6901 aaaaatcaat tactttttaa aggttattat tattgctttt actgccatag tatttcaata
     6961 gtctcttagt gccccatggc tttactttgt ttttaggaat tcctagaagc tttaatattt
     7021 tgtctaagtc tttatattcc tttacttcta tttcataata agttggaatt ctaaaaggtt
     7081 tagagatagt atcaatatca aatgttgttg tgccaagcaa ataggttgtt ctggtctttt
     7141 ctattctgtg gtatcttttt aggccaagag acttaaacat atcctgtaca gttttaaaat
     7201 cagaagaaac ttgaaattct atttctttac agcttttaat attatttctt atttccttaa
     7261 attgtttaaa agttataaaa acatctttac caagttgtct aactcttatg gctgcatttt
     7321 ttttcttaag ttctttgttg tcatagtttt cttgtattac ttttttatca gagaaacgtt
     7381 ttttcgcccc aatctctttt agtttatttt ctagttcttt tttatttact tctagaactt
     7441 ttacttctat ttcataatgg tctttactca tattctcact ctaaattaac ttataatttt
     7501 taaataactg ctctgcagtc cattttctta ttttcttttt gtctattttt aaaatcttta
     7561 tgtatttttc tattactttt atagattttg cctctatttc caaaaatgtt ggaattcctt
     7621 ttattgtatc tattccaaat attgtcccat ctttaatata aagtattcct tctttttcat
     7681 attctagttt tttaataaat agcttgtcta gaattaactt cattttatta taatcagata
     7741 ctttaacgcc aatctcttca gcaatcttta ctggtttatt tgtttttaac acttttttat
     7801 aagttataga aaaaacacct ggtttttttc ttaacctaat tattacattt ttgttttctt
     7861 tccctggaac tccatacagg atggttttta tttttctttt tgctttcttt ttcgccccaa
     7921 tctcttttag tttcttttct agttcttttt tatttacttc tagaactttt acttctatct
     7981 cgtaatggtc tttactcata ttattccttc tttacttaga ttttctaaag ctttaatcat
     8041 tgctagaggg aaagtaattg taacatctcc aacaactgtt actgcatctg cttcgtcttt
     8101 tattttcccc caggactttg cttcgtctgt tgttgctcct gaaagtgagc cggatcttga
     8161 tgacgcagta gtcatgtaaa gagcatagtc tgctcctccg ttgattagtg tagttagaat
     8221 tacatggtgc ttagagacgc ctccaccaag agcaataatt cctttcttct catcgtgtgt
     8281 tgttgaaaaa ataatctgtt taaaatcaga aataatatca atcataaaat ctttatggtc
     8341 ttgctgaaaa agatacaagt gaaaaccaaa agaaccatct gttattgcag gacaaaagac
     8401 tggaatatta ttttttgctg cttgatacag aaaagaattc tcgtcattta gttttaaacc
     8461 aatttctctt agaagctcgc aagttgacca aactttcttt tgcttgtaaa gttctaaaaa
     8521 cagtttttgc atttttcctt caagcttagc atagctttcg tttgtgatca agatgttacc
     8581 aactctgttc tctcctctct cgtaaagctc ttggtcgtct gctttaaagg atgctaaaat
     8641 aaacttctct ccatctgctt tcattatgtc ttcttcaata gccccaactg ttgtacaaac
     8701 aacgcttgcc atctttaact ttaggacttg agaaaagaac cctctaagac cagaagtaac
     8761 catgtttgag gtaaaagaaa gatagatttt ggctccttct tttttcattt tctcaataag
     8821 gttagctgct cttgatagtt ctgaagattg aaaaccagta gaagaatagc tatcaactaa
     8881 gtcagaaacg ctcattcctt ttgtccataa taagtcttta gtatatttca ta
//
LOCUS       DAOOON010000026         8575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_10192_length_8575_cov_13.974178, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000026 DAOOON010000000
VERSION     DAOOON010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10192_length_8575_cov_13.974178"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1281
                     /locus_tag="PLK55_03545"
     CDS             103..1281
                     /locus_tag="PLK55_03545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOZ36031.1"
                     /translation="MRIGIISDWHLGVDFDNELGKDSFTNLFLAFKQLETQDIDLLLV
                     PGDLFDVSKPEPEVLFEAINLLNEINIKNKYTEIKAKIPILAIIGNHEYRSKDYITTV
                     ELLESLGFLKLLKADCLKFEKEKIAIFGMTALPEKMALSYLKEKFNPQPLKGYYNILM
                     IHQSIVEYLPFEDEMVATISLSDLPSGFDVIVNGHLHWSSIIDLPQGPKFLLPGSTVT
                     TQIKQKETEKPKGYFIIDTDKKEIDFCQIKDIRKVYYIDLKFSKKTVSDIISMIKAEL
                     EKLEKTPNRKAIVRIRLKGELEDGESLKNLNIESLKKDYSEFFYISLKNEISEKRLKE
                     SIEQLKRLQSENKNVSELAKSIFSEQIEQTKISKGFDYRRIYELLENGDVDKAKGALN
                     "
     gene            1346..8356
                     /locus_tag="PLK55_03550"
     CDS             1346..8356
                     /locus_tag="PLK55_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36032.1"
                     /translation="MNLKYITLFLLIIFITPFAFAELQPAFLDQNTHYFGEKINFGLI
                     GVIPDDFNHARIIVQLPTGHSYQEADIDIDNLSDYYLVFPLDFIYNQLQIKYIFYNSN
                     NTAPLNTEYTLQLERLPLIENINYCINADCNKNNFEVIDANEIYLKTQTEIIDENLNF
                     DILVILDGYIIEDGRNIQLPYLLDTTPGEYEIKVTPNYNNQELETKSLFLNLAITQPE
                     TNNLEDYLNDIREQNEAEEREQEINTAIENINDSQTTETQEKTEPKNNLWLIGLGILV
                     VLIILISILLFSRPKEKFVEKPSRRESRKERGFLFLIIFVLLFSSIYGLSLEEKIKEY
                     QNLEITFDTLQKSYPYPKDISIPISNTQPQNVFYYPISATGLSDAEKITPIAENKLSN
                     LIVSLETVYLSIDLSAEIETAKTTGKTLGFESGCEQVLEWFQKNPITAEPNTIIARTK
                     ENLLNNITITENKTGYTDNDKTQIRDKIKKLKTDKKYKNSKNPFVEYCISDYLENKES
                     NIGGKYITKKKILLKMEYTEKNLEAINNYDLTFDNIIFNDSKGKDVLETTFLGSDLNH
                     IYVWVDYTADILREQNLSLKGIAANRILQEEIADNGEIIKHYMITNLDEGESVDVTYA
                     AQLINLEILDSDYGSYYQFEGVGQDTSATPEKKGNVFAIRDKDLFSVKNKILSNVEIS
                     GKISLENGNYKFELSPESIKLLAEVNINPTKYIVLDKKQLIDVGTDTQKLTIGIDYNY
                     GIICSDFSGITILLEEDKTLYLPTNINNPHLEALTKLFIKTSSQKNIELTRLQLPLLF
                     IFAFKNNANTIPDEDVLFLKTYDDVANIINRGNYTKLEKNIIFNNLISLTEDFSFQKT
                     ANSKDGLYRFKYKTRFVLNYGLVNYIDHPENINNLNLETVGITIPQLYDNKYLERKGF
                     VDKSLTTLLVLGYLGDNYRSFSKVIRSSYNSHEEYEIDLKNQIQTVFNEQKATTDNWG
                     YYKELLIYPFYRTGIEQKICSYDKENFPKYSVKNYCYQAYNFNPESNTWGIAYDPHNL
                     DSGFLMQDYSFQNNFVSSYRTILLLEDIENFKNNKLTFNQNQLKTYNKLNIFLSLIEL
                     EAIRNTSTTENNRNFGNALILSYKLRELWDSSSNELKKYDSGTIRNAIINTNEALAYQ
                     ATVKLRNVVSTDKSLRLDIYKGLIQTDFERYAESINPSAGWFDLSDSARFLITGSWIE
                     TFIFTGSRKEIESLDQKERDKYNKILHGSCCIAQRLGELESDSSYKNWTIKDIKDNFS
                     PYSKAKTNTKTDVIGWVTDGTSCTGTLRANPGDCATDISYLYNSKEFGNDISFILNDK
                     KDKKSITQFLSAGYYTNHTLDMDSSIQVNSTKRELEETKVKFKPDAITSALRFIDSGI
                     NPATIIITVATAGAGYGLMTGTGSVFTRIASGFTTKLTLGKLSSALGTRLLYEFGENI
                     VLDAAYGAIVIPSPKLAGEHPFLTYVVLGVIAGNFQKAFISNVDNAASAITKDLIATS
                     GLKQADNIKIQTAIKTALNTDYSDTTEQLLKIKRGEAVTDSFFRRLPKVVAGTADDEL
                     VTAIRKNLLSQKISVSDDVIRKSLTQNNLYLGTRASAVSKFISRKNLIELNNIAAANL
                     DNTSSKFIKLATTRTVPERYGAKFTSIVDEFEDLKKFNEIDPEHFAKPTKIVYDDFGN
                     PLGYEMEKIYGTTLADYIEDGGVLDDSFYKRIEDSVSELHKQGYAHGDINPNNIILRE
                     TYDGLDFTIIDPVGFRKLNQITDIPGLTEQAAIDLDIEQLNKLRNLTILEAEMMRTNK
                     IYVPNVWELQRIQQTGELVPIRREGGNIVLGEVLEISSDNTKVKVRFSDYLNNVTDER
                     FYDIDTETFIPIRQNDIVYVNGVGSGKVITVEPNKTVTLRRTDGSIIYTDVDAIISSR
                     SLNQGLKLNGDISQITPQTTEAFSATKNRLPERFISRNTDSWSGREVILVDKINDPVL
                     KEYLANVRILSSQNPDILNKIKEAYNLVNRTMPVYSLGKTNILDDIIDNVDVGGDVIP
                     LGHFINSKIGVCRHKALLLKLTLDEMGIESYLVRGSQYTDEGKNLGRHVWVEMDLKTN
                     KGNKIYLDPTQNKYEYFYDGQQVNGLVRDSELTLFRIRDTVPQNNFRNLADGADIYKR
                     TARLQNYLTEELHNYNLNPTAINDFTFKYNITQQITNPQFLSQIGSNLNDLQDLMKLQ
                     NLKIKEIYQNDGLLLTLNHKLSLSSLPTNVREEIVSELGRKNVSVIVVDDWTIRNLDD
                     QDNFATLNLNDPRNPIIKIERSAYNDPNKFLREMRYEYGALLLSKQYGSKGDIPLFFK
                     PSDAGKIEPVWATHYLDHYMEKGYNRLFPN"
BASE COUNT         3425 a         1166 c         1335 g         2649 t
ORIGIN      
        1 gcataaccgg tctgccatca aggcgcttat tctttatata attaagaaca aatatatttt
       61 taaaggaaac aattattaaa aagttctgtt atattattat ttatgagaat tggtataatt
      121 agcgattggc acctgggtgt tgattttgat aatgaacttg gaaaggactc ttttactaat
      181 ctttttttag catttaagca gcttgaaaca caagatattg atcttctttt ggttcctgga
      241 gacctctttg atgtctcaaa accagaacca gaggtgcttt ttgaagctat taatctttta
      301 aatgaaatta atattaaaaa caaatatact gaaattaaag caaaaatacc aatacttgca
      361 attattggaa atcacgaata tcgctcaaag gactacatta caactgttga gcttttagag
      421 tctcttgggt ttttaaaact tttaaaagca gactgcttga aatttgaaaa agaaaaaata
      481 gcaatctttg gaatgactgc tcttcctgaa aaaatggctc ttagctatct taaagagaag
      541 tttaatccac agcctttgaa aggatattac aatatcttaa tgatacatca atcaattgtt
      601 gaatatctgc catttgaaga cgagatggtt gctacaattt ctctttctga tctaccttct
      661 ggatttgatg taattgttaa tggccacctt cactggtcaa gcattattga tctaccacaa
      721 ggccctaagt ttcttttacc tggttcaact gttacaacac agattaaaca aaaagaaaca
      781 gaaaagccaa aaggctattt tattattgat actgacaaaa aagaaattga tttttgtcaa
      841 ataaaagaca ttagaaaagt atattatatt gatttaaagt tttctaaaaa gactgtttct
      901 gatattattt ctatgataaa agcagaactt gaaaaacttg agaaaacacc aaacaggaaa
      961 gcaattgtta gaatcagatt aaagggagaa ctagaagatg gagaatctct aaagaacctt
     1021 aatattgaat ctttaaaaaa agactactct gaattctttt atatctcttt aaaaaacgaa
     1081 atttcagaaa aaagactaaa agaaagtatt gaacaactaa aaagattgca aagcgaaaac
     1141 aagaatgttt cagaacttgc aaaatccata ttttctgagc agattgagca aacaaaaatc
     1201 tccaaaggct ttgattatag aagaatctac gagcttttag aaaatggcga cgtagataaa
     1261 gcaaaaggcg ctcttaacta gtcttcttta tattttataa atatatctta gcatatttct
     1321 tttaactata taaagagaaa taattatgaa tttaaaatat ataactttat tcttattaat
     1381 aatatttata acaccttttg cttttgcgga attacaaccc gcattcttag atcaaaacac
     1441 gcactatttt ggagaaaaga ttaatttcgg attgattgga gttatccctg atgatttcaa
     1501 tcacgcaaga attattgtgc aattgccaac aggtcacagt tatcaagaag cagacataga
     1561 tatagataat ttaagtgatt attatcttgt ttttccatta gattttatat ataatcaatt
     1621 acaaataaaa tatatttttt ataattctaa caatacggcg ccccttaata cagaatacac
     1681 tttacaatta gaaagattgc ctctaattga aaatataaac tattgtataa acgcagattg
     1741 taataaaaat aactttgaag taatagatgc aaatgaaatt tatcttaaga cgcagactga
     1801 aattattgat gaaaatttaa attttgatat attagtgatt ttagatggtt atattataga
     1861 agatgggaga aatatacaat taccttatct tttagatact actcccggag aatatgaaat
     1921 taaagtaact cctaattata ataatcaaga attggaaact aaatctttat ttttaaattt
     1981 ggctatcaca caaccagaaa caaataactt agaagattat ttaaatgata ttagagagca
     2041 aaatgaagca gaagaaagag aacaagaaat aaataccgct atagaaaata ttaacgactc
     2101 tcaaacaacc gaaactcaag aaaaaacaga acctaaaaat aatctttggt taattggatt
     2161 ggggatttta gtagtattga taattttgat ttcaatttta ttatttagta gacctaaaga
     2221 aaaatttgtt gaaaaaccaa gcagaagaga aagccgaaaa gaaagagggt ttttattttt
     2281 gataatcttt gtgttgttat tttcttctat ttatggtctg tctttagaag aaaagattaa
     2341 agaatatcaa aatttagaaa ttacttttga tactctgcaa aaatcctatc cttatcctaa
     2401 ggacatatct ataccaataa gtaatactca accacaaaac gttttttatt accctatttc
     2461 tgcaactgga ctttcagacg ctgaaaaaat aacgccgata gccgaaaaca aactatcaaa
     2521 tctaattgtg tctttagaaa cagtttactt atctatagat ttgtctgcag aaatagaaac
     2581 tgcaaaaacc actggtaaga ccttgggatt tgaaagtggt tgtgaacaag tattagaatg
     2641 gttccagaaa aatccaataa ctgcagaacc aaacactata attgccagaa ccaaagaaaa
     2701 tttgctaaac aacataacaa taactgaaaa taaaacagga tataccgata acgacaaaac
     2761 acaaattaga gacaaaataa aaaaattaaa gacagataaa aaatataaaa actctaaaaa
     2821 tccatttgtc gagtattgta tttcagacta tttagaaaat aaagagtcaa acattggtgg
     2881 aaaatacatc acaaaaaaga aaatattatt aaaaatggaa tatacagaga aaaaccttga
     2941 ggccataaat aattatgact taacttttga caatataata tttaacgatt caaaaggaaa
     3001 agatgtactt gagacgacct tccttggaag cgatttaaat cacatatatg tttgggtgga
     3061 ttatactgca gatattttgc gtgaacaaaa ccttagccta aaaggaattg cagcaaatag
     3121 aatattacaa gaagaaatcg ccgataatgg cgaaatcatt aagcactata tgataactaa
     3181 tcttgatgaa ggagaatcgg tagacgttac ctatgctgct caattgataa atctggaaat
     3241 attagattcg gattatggct cttattatca atttgagggt gttggtcaag atacttctgc
     3301 aactccagaa aagaaaggaa atgtctttgc tattagagac aaggatttgt tttcggtaaa
     3361 aaacaaaata ttaagcaatg ttgaaatctc tggaaaaata tccttagaaa acggaaatta
     3421 taaatttgaa ttaagtcccg aatctataaa attacttgca gaagtaaata taaatccaac
     3481 aaagtatatt gttttagaca aaaaacagtt aattgatgtt ggaacagata ctcaaaaact
     3541 tactatagga atagactata attatggaat tatttgtagt gacttctccg gaatcacaat
     3601 tttattagaa gaagataaaa cactttatct gccaacaaat attaataatc ctcatttaga
     3661 ggcactaact aaattattta taaaaaccag ttctcaaaaa aatatagaat tgacaagatt
     3721 acagttacct ttgcttttta tttttgcttt taaaaacaat gcaaatacaa tccctgacga
     3781 agatgtttta tttttaaaaa cttatgatga tgttgcaaac ataattaaca gaggtaatta
     3841 caccaagttg gaaaagaaca taatatttaa caatcttatt tctttaactg aagatttcag
     3901 ttttcaaaaa accgcaaatt ctaaagacgg actatataga tttaaatata aaaccaggtt
     3961 tgttcttaat tatggacttg taaactatat cgatcatcca gaaaatataa ataatttaaa
     4021 tttagaaact gtaggaataa ctatccctca attatatgac aataaatatc ttgaaagaaa
     4081 ggggtttgtg gataaatcat taacaacact cttggtgctt ggctatcttg gggataatta
     4141 tagatccttt agtaaagtaa ttagatcaag ctataattct catgaagaat acgaaataga
     4201 tttaaaaaat caaatacaaa cggtgtttaa tgaacaaaaa gcaacaacag ataattgggg
     4261 atattataaa gaattattga tttatccatt ttacagaact ggaattgaac aaaaaatctg
     4321 ctcatatgat aaagaaaact ttccaaaata ttctgtaaaa aactactgtt atcaggcata
     4381 taattttaat cctgaatcta atacttgggg aatagcatat gacccgcata acctagattc
     4441 tggattttta atgcaagact attctttcca aaataatttt gtttcatcat atagaacaat
     4501 attgttgctt gaagatattg aaaactttaa aaataacaaa cttacattta atcaaaatca
     4561 attaaaaaca tataataaat taaatatttt cttaagttta attgaacttg aagctataag
     4621 aaatacttca acaaccgaaa ataatagaaa ctttggaaat gctttaattt tatcttataa
     4681 actaagagag ttgtgggata gtagtagtaa cgaacttaaa aaatatgatt cgggcactat
     4741 tagaaacgca attataaata caaacgaggc attagcatat caagcaaccg ttaagttaag
     4801 aaatgtagtt tctactgata aatctttaag attagatata tataaaggtc ttatccaaac
     4861 agattttgaa agatatgctg aatctataaa tccttctgct gggtggtttg acttaagcga
     4921 ttctgcgaga tttttaatta ctggcagttg gattgaaacc tttattttta ctggtagccg
     4981 aaaagaaatt gaaagtttag atcaaaaaga aagagacaaa tataataaaa tactacacgg
     5041 atcctgttgt atagctcaaa gactaggaga attagaaagt gattcaagtt ataaaaattg
     5101 gacaatcaaa gacataaaag ataactttag tccttattct aaagctaaaa caaatacaaa
     5161 aacagatgtt attgggtggg taactgatgg aacaagttgt actggtactt taagagcaaa
     5221 ccccggggat tgtgctactg atatatctta tttatataat tccaaagaat ttggaaatga
     5281 tatttctttt atattaaatg acaaaaaaga caaaaaatct attactcaat ttttaagtgc
     5341 tggatattat actaatcaca cattagatat ggattcttct attcaagtta attccacaaa
     5401 aagagaatta gaagaaacaa aagttaaatt taaaccagat gcaataacct ctgcattaag
     5461 atttattgat tccgggataa accctgcaac aattattatt accgttgcaa ctgctggagc
     5521 aggttatgga cttatgacgg gaacgggcag cgtcttcaca agaattgcaa gtggatttac
     5581 aacaaagctg actttgggaa aattatctag tgctttaggg acaagattat tgtatgaatt
     5641 tggagaaaat atagttttag atgcggctta tggtgcaatt gttattccaa gtcctaaact
     5701 tgcaggagaa catccattcc tgacatatgt ggtacttgga gtaatagcag gaaacttcca
     5761 aaaagcattt atatcaaacg tagataatgc cgcctcggca attacaaaag atcttattgc
     5821 aacatctggt ttaaaacaag cagataacat aaaaatacaa acggcaataa aaactgcgtt
     5881 aaatacagat tattcagaca ccacagaaca attactaaaa ataaaaagag gagaagcagt
     5941 aactgattcc ttttttagaa ggttaccaaa agttgtagca ggcactgctg atgacgagct
     6001 tgtaactgct attagaaaaa atttattatc tcaaaagata tcggttagtg atgatgtaat
     6061 tagaaaaagt ttaactcaaa ataatttata tcttggaaca agggcttctg cagtatctaa
     6121 atttatatct cgcaaaaatc ttatagagtt aaataatatc gctgcagcca atttagacaa
     6181 cacaagtagt aaattcatta aattagcaac aacaagaact gttcctgaaa gatatggtgc
     6241 aaaatttaca agtattgttg atgaatttga agatttaaag aagtttaatg aaatcgaccc
     6301 ggaacatttt gctaaaccta caaaaatagt ttatgatgat ttcggaaacc ctcttggcta
     6361 tgaaatggaa aaaatatatg gcacaaccct tgcagactat attgaagatg gaggagtatt
     6421 agatgattct ttctataaaa gaattgaaga ttcagtaagt gaattacata aacaaggcta
     6481 cgcacacgga gatatcaatc caaacaatat aatacttaga gaaacatatg acggattaga
     6541 ttttactatt attgatcctg ttggttttag aaaattaaat caaattacag atattccagg
     6601 attaactgag caagcagcaa ttgacttaga tatagaacag ttaaataaat taagaaattt
     6661 aacaattcta gaagcagaaa tgatgagaac aaacaaaatc tatgttccta atgtttggga
     6721 attacaaaga atacaacaaa ccggagaatt ggtgccaata agaagagaag gcggtaacat
     6781 tgtattaggc gaagttttag aaatatcttc agacaataca aaagtgaaag ttagattttc
     6841 ggattatcta aataatgtaa ctgacgaacg tttttatgat attgatactg aaacatttat
     6901 accaataaga caaaatgaca tagtttatgt aaatggtgtg ggctcaggaa aagtaataac
     6961 agttgaacca aataaaacag taacattaag aagaactgac gggtccatta tctatactga
     7021 tgttgatgca ataattagtt ctaggtcttt aaatcaagga ttaaaattaa atggagatat
     7081 atctcaaatt actccacaaa caacagaagc attttcggct acaaaaaata gattaccaga
     7141 aagatttatt tctagaaata ccgattcttg gtctggaaga gaagttattt tagttgataa
     7201 aataaatgac cccgtgttaa aagaatatct tgcaaatgta agaatattaa gctcacaaaa
     7261 tccagacatt ttaaataaaa tcaaggaagc ctataattta gtaaatagaa caatgccagt
     7321 ttatagttta ggaaaaacaa atatcttaga tgatataata gataatgtag atgtcggagg
     7381 agacgtaatt ccattaggcc attttataaa ttcgaagatt ggggtttgta gacacaaggc
     7441 gctattatta aaacttaccc ttgatgaaat gggaatagaa tcttatcttg tcagaggatc
     7501 acaatataca gatgagggta aaaatttggg caggcatgtt tgggtagaaa tggaccttaa
     7561 aacaaacaaa ggaaataaaa tttatttaga tccaacacaa aataaatatg aatattttta
     7621 tgatggtcag caagttaatg gattagttag agattcagaa ttgacattat tcagaatcag
     7681 agatactgtt ccacagaata actttcgaaa tttagccgat ggagcagaca tatacaaacg
     7741 aactgcaaga ttacaaaatt atttaactga agaattacac aattataacc taaatccaac
     7801 agcaataaat gattttacat ttaaatataa tataactcaa caaattacaa atccacaatt
     7861 tttatcacaa ataggatcta atttaaatga tctacaagat ttaatgaaat tacaaaattt
     7921 aaaaattaaa gaaatttatc aaaatgatgg acttttactt acattaaatc acaaattatc
     7981 tttgtcgtca cttccaacaa atgtgcgtga agaaatagtt tctgaattgg gcagaaaaaa
     8041 tgtgtcagtc atagttgttg atgattggac gattcggaat ttagatgatc aagataattt
     8101 tgctacactt aacttaaatg atcctagaaa tccaattata aaaattgaaa gatcagcata
     8161 taatgaccct aataaatttt taagagaaat gagatacgaa tatggtgcat tattgctttc
     8221 taaacaatat ggtagtaaag gagacattcc tttattcttt aaaccatctg atgccgggaa
     8281 aatcgaacct gtctgggcaa cacattattt agatcattat atggagaaag gatataatag
     8341 attatttcca aattaagtaa atcaaaacct atatatatgg gtttgatcat ataattatat
     8401 atgaaatgct tatcatataa cttcttttcg gttattggaa atgaacttcg gtggaagatt
     8461 attaaaaaac tttataagtc agaattgact gtttctgaat tgtgtgttgc tcttgaagag
     8521 gaacagtcta aagttagcca tgctttaaag atactacttg attgtaatat tgttt
//
LOCUS       DAOOON010000027         8544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_10242_length_8544_cov_22.139475, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000027 DAOOON010000000
VERSION     DAOOON010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10242_length_8544_cov_22.139475"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1615)
                     /locus_tag="PLK55_03555"
     CDS             complement(5..1615)
                     /locus_tag="PLK55_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36033.1"
                     /translation="MSKKGQVALEVLVVISVIIIGMVLFGMFYLNQHSAQFSMSKSDS
                     VLGMAQSPINYSEVTSPTRIICGNFICESGETCENCSSDCCTENCGNGNIDSGEECDS
                     LNFGVQTCSSLIPDSSGNLSCNLDCTINTSGCVALPVCGNGIIEGAEQCDGEDLNSES
                     CSSLNFDYGALSCNESCLFDTTQCEFWPASECLKFESGGWRPYNTTDPNWFTRGNMAT
                     WPDPSALWLCPSSDCHYIQDYYFLYNSFVLSETTNVIISALGDDETKVWLWPDHDISN
                     EIVIIPKYVWINGWASDSRELLAGEYSIVQLIYDTNQSATGAILSVENSNTNEIILNT
                     NLTSGWRYYSTMTQLEEERFSSNNGCSRVYSNQMCGNHIIEGSEQCDGEDLGEFSGKT
                     CTDVNSSWLIGDLSCNSNYCQIDTSNCKKAIELILTPESGSIRRNRNFNTVLSVDGED
                     GRAFNLVASIKLKNAFTGNYEPTNNCKYILTGVYSSTFDLGNNSIPITNKTYSFNCKS
                     SGDYMITFKGTLVDGTSSEDSSLWKITN"
     gene            complement(1712..4399)
                     /locus_tag="PLK55_03560"
     CDS             complement(1712..4399)
                     /locus_tag="PLK55_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36034.1"
                     /translation="MRKEYLVSLLFLVAIVISGVYADPFAINYYVNTDSSQEYLNIQY
                     GNYYDNVSLRLRLQAMPGYSNVPVVVSPKVYGLDSYGNLTYLYSVPSQTYYSYSTPYE
                     YVYPNVFYLTPDYYGYVVKVYAEATGYYYNTYSYAYVYPVNNAPYYYFIEDTPTDDDF
                     DFPQNTTPDCSDIVLSGTQDIYLDESESDTYSFYIENEANEDLEIVDVYTSDPAKLDI
                     DDIDYPDEVPDLSVRTVRMDIEADSVSDDYDSVFTITVKARYPNSSICTKTYEVEYHI
                     SEEDNDDDDDDEEDVSCSDLDITNTRFTINDDETSTRKIKLENDSDDYDFEIDDITIS
                     DRDGLDVSIEDWPDEIDSDDYEYLEIELDADDFDYDITKYLTLEVEGTFVDGSDEIRE
                     CTRKATITVTIEDDGDNDSDDDDDDETSCDDIYLYTMNISQAENSTDYFSEEDGFYIL
                     NNSDRTFTITSLSLNDNSSFVSINNLNYSKTISARDTSSLNFNLKTINVSTTEYARGT
                     LLVAGRFSDGKTCSSSNIGTEYFDIIVNNLTSGSTPTNNTSTNNGSNFSGSSCSIDII
                     APSTVSVNNSQESINVSFKNYTSKNGRIEITSNGLTTNPSVIFLSGNDNFSQNITLSN
                     FNNPTNITYTAYLNGCASKSTFTNIINNISESERITLVSYPTFISPLSNNPKISVNVN
                     NSFTVSKEVTLKLAGLPNTFSSSPISVTLSAHQTKQVDLDLSIPQSAQKIDYQAYIEL
                     YSGSLLVNKYPITISLSPTTSEITIVSSSKQSATQDRTYTITLTLRNNTNNIQEGVVD
                     FGLPDTYVIDGEKNIYLLPNEEITKIYKIVSPTRLREDKVLDIKIKDKATGKELANEK
                     LTLDSGKSPLSAFFSLKNAGSILLGIVVLIVLFLVFRKR"
     gene            4607..4993
                     /locus_tag="PLK55_03565"
     CDS             4607..4993
                     /locus_tag="PLK55_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36035.1"
                     /translation="MVEENSRTSQDVKALQSSILVLSEKIKYLVHNEKILSRNILVLN
                     KKVAAKGDGGSGINAATNEQFKELQADLKELKTKIRAIESTLEMISRRYAKAEDLKEM
                     KYIVENINPLEYTTVDQVKKLIKENK"
     gene            complement(5002..5442)
                     /locus_tag="PLK55_03570"
     CDS             complement(5002..5442)
                     /locus_tag="PLK55_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36036.1"
                     /translation="MGSRKNNRKTVVRKKKHLTEEQNIEELEKSKKKKFDNQKVPLLK
                     QKEVSEVIDHVLQGLKRSSHGLNIGEITREHKVKIYLLFEELRDDPKNQKTLARTNKS
                     DLEIADVLVDYIKAKNKTIDEILSQMSRKEIDLINKLIFKKLFF"
     gene            complement(5444..6232)
                     /locus_tag="PLK55_03575"
     CDS             complement(5444..6232)
                     /locus_tag="PLK55_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36037.1"
                     /translation="MPKPKIKIHTKGNPITPLPEKNKVIFKKVIPTIRKIQEKRYWAE
                     DAPGIQSEIDRKVNPKLKILRRMVGVKTLAVLSKKGQKDLNNFWYKRLKDLPEITFED
                     LKKLKHEAFSIIIKVKNQKFKNNLISKADAKKRLIQEELEDYDLLKATDKKRINTIIN
                     YNLALLNSNSLISFEDYRELLNTVNMEYNKFNLRYVVAKKIKTALLSNPRTKELLSYR
                     SVIEQKKLFKYLDTLIREEVNIYSIKSQEIIKRQFISKILGLKK"
     gene            6403..6645
                     /locus_tag="PLK55_03580"
     CDS             6403..6645
                     /locus_tag="PLK55_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36038.1"
                     /translation="MTGIARSAPNILFFREIPTDERIAEIRRENSKMPEQRKEITIGS
                     FRLDTRMLEVLVRESKTKELLAPKTDNNTLEKKPKK"
     gene            complement(6794..7189)
                     /locus_tag="PLK55_03585"
     CDS             complement(6794..7189)
                     /locus_tag="PLK55_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HOZ36039.1"
                     /translation="MVEWKRVSRRKATGGVNNAVNAKTKSLSEKGGKFSKTTVAKVDK
                     RFKTRTVGGNKKIKISHAQFVLVSEGNKTIKAKVIDVTENPANKHFVRQKIITKGAIL
                     KVDVDGKETRVKVTSRPGQSGQVTGVFVK"
     gene            7295..7873
                     /locus_tag="PLK55_03590"
     CDS             7295..7873
                     /locus_tag="PLK55_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36040.1"
                     /translation="MAKPPIPRRVIKSRQTIPQSKIKPILKSNTEKKYENRVSIAHEN
                     ILEVFEKMDFLKDLIDSDPEFVRQFHDARKNLKSLGFSGSIDNAIYQQLLYAKVARII
                     KKDPYSTRERIQKINKKMNEIYNNITTIESLHEKRKPHLSKEQLTKLEFEKNAKILEL
                     KKQYSELLEEKQHIKKLLKLTIKQIEELENIR"
     gene            complement(7874..>8544)
                     /locus_tag="PLK55_03595"
     CDS             complement(7874..>8544)
                     /locus_tag="PLK55_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HOZ36041.1"
                     /translation="MPQKLKIVCDTRERVSQINQELKNLSNDSFEVEVLIEQLSLGDY
                     QLSDQVLIERKTIADLESSIIDGRIFSQLDSLSEKVSKPGLIIEGIINFSDNGNRINN
                     KSLIGLITSIGLNYRIPIFFTRNQKETALFLYVIAKREQYGVKTEIKHRFSKNKMSFS
                     DQQLFILESFPDIGPTLAKSLLKRFKTIRNISQASVKELQEVEKMGPKKAEKMHYLFS
                     RVFD"
BASE COUNT         2906 a         1387 c         1231 g         3020 t
ORIGIN      
        1 taatttaatt tgtaattttc cataaagatg aatcttcaga agatgttcca tccacaagtg
       61 tgcctttaaa agtaatcatg taatcgccac tacttttaca attaaaacta tatgtcttat
      121 ttgttattgg aatagaattg ttgcctaaat caaatgtact agaataaact cctgttaaaa
      181 tatacttaca gttgtttgtg ggttcataat tacctgtaaa cgcatttttt aatttaattg
      241 aagcaactaa attaaaagct cttccgtctt cgccgtctac cgaaagcaca gtattaaaat
      301 tacgatttct tctaatactt ccactctctg gagtaagaat taattctatt gcttttttac
      361 agttacttgt atcgatttgg caataatttg aattacaact taaatctccg attaaccaag
      421 aagaattgac gtctgtacat gtttttccag aaaattctcc cagatcttcg ccatcacatt
      481 gctcgctgcc ttcaattatg tgattaccac acatttgatt agaatatact cttgaacaac
      541 cattatttga agaaaatctc tcttcttcta attgagtcat agtactataa taacgccaac
      601 cagaagttaa atttgtattt aaaataattt cattagtatt tgaattttca acagacagaa
      661 ttgcacctgt tgctgattgg tttgtgtcgt atattaattg aactatagaa tactctccag
      721 ctaataattc tctagaatca gaagcccaac catttatcca aacgtacttt ggaataatta
      781 caatctcgtt tgagatatca tgatctggcc acaaccaaac ctttgtttca tcatcaccaa
      841 gagcagaaat tataacattt gtagtttctg ataaaacaaa agaattatac aaaaaataat
      901 aatcctgtat ataatggcaa tcggaacttg ggcaaagcca aagtgcactt ggatctggcc
      961 atgtcgccat gtttcctctg gtaaaccagt tagggtctgt ggtgttataa ggccgccaac
     1021 cgccgctctc aaatttaaga cactcacttg ctggccagaa ttcacattgt gttgtatcaa
     1081 acaaacaact ttcattacaa gatagagctc cataatcaaa atttaaagaa ctacaacttt
     1141 cgctatttaa atcttcgcca tcacattgct ctgcaccctc tataatacca ttaccacaaa
     1201 ctggcaaagc cacacagcca gaagtattaa ttgtgcaatc gagattacaa gataaattcc
     1261 cagaagaatc gggaattaaa gaagaacaag tttgtactcc gaaattcaaa gaatcacact
     1321 cttctccaga atcaatattt ccattaccac aattttctgt acaacaatca ctagaacagt
     1381 tttcacaagt ctctcctgat tcacaaataa aattaccaca gataattctg gttggagaag
     1441 ttacctctga ataattaatg ggtgattggg ccatacctaa aacactatcg gacttcgaca
     1501 tagaaaattg agcagaatgt tgatttagat aaaacatacc aaaaagaacc ataccaataa
     1561 ttattacact aattacaacc aaaacttcta atgcaacttg gcctttttta ctcatagcta
     1621 tacacctccc ccagaaacta caaatatatt taggcaatac ttttttatat acttcttttt
     1681 aaaaaaatag cagaaaagag aaagaaaaat cttatctctt tctaaagact aaaaatagaa
     1741 caataagcac aacaataccc aaaagaatag agccagcatt ctttaaagaa aagaaagcac
     1801 ttaatggcga tttaccagaa tctaaagtaa gcttttcatt tgctaactct ttacctgttg
     1861 ccttgtcttt tatctttatg tctagaactt tatcttctct taatcttgtt ggagaaacaa
     1921 ttttatagat ttttgtaatc tcttcgtttg gaagaagata aatatttttc tcaccatcaa
     1981 tcacatatgt gtctggtaga ccaaaatcaa ctacgccttc ttgaatatta tttgtgttgt
     2041 ttcttaaagt taaagtgatg gtataagttc tgtcttgagt tgcagactgc ttagaactag
     2101 acactattgt tatctctgaa gttgttggag aaagactaat agttattgga tatttattaa
     2161 ctaaaagact accagaatat aactctatat atgcttgata atcaattttt tgagcacttt
     2221 gtggaatact taaatctaaa tcaacttgct ttgtttgatg tgccgaaaga gtcacagata
     2281 taggagaaga actaaaagtg tttggaagcc ctgcaagttt taaagttact tcttttgaaa
     2341 ccgtaaaact attatttaca tttactgaaa tctttggatt gtttgataat gggctaataa
     2401 aggttggata ggaaactaaa gttattctct ctgattcaga aatattgttg attatgtttg
     2461 taaaggttga ctttgaggcg caaccattta gataagcagt ataggttatg tttgttggat
     2521 tattgaaatt tgaaagagtg atattttgtg aaaaattatc attgccgctt aagaaaataa
     2581 cacttgggtt tgtggttaaa ccgtttgaag ttatttcaat tcttccgttt ttgcttgtat
     2641 aatttttaaa agaaacattt attgattctt ggctattgtt tacagaaact gttgatggcg
     2701 caataatgtc aatactacaa cttgaaccag agaaattgga gccgttgttt gttgaagtgt
     2761 tgtttgtcgg ggtagatcct gaagttaaat tgttaacaat tatatcaaaa tattctgtgc
     2821 caatatttga agaactacag gttttgccat cagaaaatct accagcaact aaaagcgtac
     2881 ctcttgcgta ttctgttgta cttacattta ttgtttttaa attaaaattt aaagaactag
     2941 tgtctcttgc acttattgtt tttgaatagt ttagattgtt tatacttaca aaacttgaat
     3001 tgtcatttaa acttagactt gttattgtaa atgttctatc tgagttattt aaaatataga
     3061 aaccatcttc ttctgaaaaa taatcagtac tgttctctgc ttgactaata ttcatggtat
     3121 agagataaat atcgtcacag cttgtttcat cgtcgtcatc atcatcagaa tcgttgtctc
     3181 catcgtcttc aattgttact gtgattgttg cttttcttgt acattctctt atctcgtcag
     3241 agccatcaac aaaagtgcct tctacttcta aggttaagta ttttgttata tcataatcaa
     3301 aatcatcagc atcaagttct atttctagat actcgtagtc atctgaatct atttcgtctg
     3361 gccagtcttc aattgaaaca tctagaccgt ctctatcgct tattgttatg tcatcaatct
     3421 caaagtcgta atcatctgaa tcattttcta gttttatttt tcttgtgctt gtctcgtcat
     3481 cattaattgt aaagcgggtg tttgtgatat ctaggtctga acaactaacg tcctcttcgt
     3541 catcatcgtc gtcatcattg tcttcttcac ttatgtgata ttctacttcg taagtttttg
     3601 tacagattga agagtttgga tatcttgctt taactgtaat tgtaaaaaca gaatcataat
     3661 cgtcacttac tgaatctgct tctatatcca ttcttactgt tcttactgaa agatcaggaa
     3721 cctcgtcagg ataatctata tcatcaatat ctagttttgc tggatcgctt gtatagacat
     3781 caacaatctc cagatcctcg tttgcttcgt tttctatata gaagctatag gtgtctgatt
     3841 cgctttcatc aagatagata tcttgagttc ctgaaagaac tatgtctgaa cagtcaggag
     3901 ttgtattttg tggaaaatca aaatcatcat ctgttggggt gtcttctata aagtaataat
     3961 aaggtgcgtt atttactgga taaacataag catagctgta tgtgttatag taataaccag
     4021 ttgcctctgc ataaacctta accacataac cataatagtc aggagttaga tagaaaacat
     4081 ttggataaac atactcgtaa ggggttgaat aactataata ggtttggctt ggaacagaat
     4141 ataggtaggt tagattacca taggaatcaa ggccataaac ctttggagaa acaacaactg
     4201 gaacatttga ataacccggc attgcttgaa gccttaatct taaactaaca ttatcataat
     4261 agtttccata ttgaatattt agatactctt gggaactatc agtatttaca taataattaa
     4321 ttgcaaacgg atctgcataa actccagaaa taactattgc aactaaaaat aacaaactaa
     4381 ccaaatattc ttttctcata atatttttct ccataattta aaaatgatat agaaaaaagg
     4441 aaaaggtgtt ccttttttca aattggggga ttattaactt tgtctaatta ataaatatct
     4501 attgagacaa aagctttaaa aagcttatga atttagaaaa acaattgctt tttaaatatt
     4561 tcaagtatct attaaatata tttacgcaaa cattaaggta actatcatgg ttgaagaaaa
     4621 ctctagaact tctcaggatg tcaaagcatt acagtcaagc atacttgtgt tatctgagaa
     4681 aataaaatat cttgtgcata acgagaagat acttagtaga aacatacttg ttctaaataa
     4741 aaaagtagca gcaaaaggag acggtggatc aggaattaat gctgcaacaa atgaacagtt
     4801 taaggaatta caagcagacc taaaagaact aaaaacaaag attagggcaa ttgaatcaac
     4861 tcttgagatg atatctagaa gatatgctaa agccgaagac ctaaaagaaa tgaaatatat
     4921 cgtagaaaat attaatcctt tggaatatac aactgttgac caggtaaaaa aactaatcaa
     4981 agaaaataaa tagttttatt atcagaaaaa caatttcttg aaaatcaatt tatttattag
     5041 gtctatttct tttctggaca tttgacttaa tatctcgtca attgttttat tctttgcttt
     5101 aatgtaatca acaaggacat ctgcaatttc taaatcagat ttatttgttc ttgcaagagt
     5161 tttctgattt tttggatcat ctcttaattc ttcaaataac aaatagattt ttactttgtg
     5221 ttctcttgta atctccccta tatttagacc gtgagaactt ctttttaggc cttgaaggac
     5281 atggtctata acttcagaaa cttctttttg tttaagcaat ggaacttttt gattatcaaa
     5341 ctttttcttt ttgctttttt ctagttcttc tatattttgt tcttctgtta aatgcttttt
     5401 ttttctaaca actgtttttc tattattttt tctgctgccc atattatttt ttcaacccta
     5461 atattttgga tataaattgc cttttaataa tttcttgtga tttaatagaa tatatattga
     5521 cttcttctct tattaatgtg tctaaatatt taaataattt tttttgctca attacagatc
     5581 tataagacaa cagttctttt gtccgaggat tagataataa cgcagtttta atttttttag
     5641 caaccacgta tctcaaatta aacttattgt attccatatt tactgtattt aataactctc
     5701 tatagtcctc aaaagaaatc aaagaattag aattaagtag tgccaaatta taattgatta
     5761 tggtgttaat tcttttttta tctgtggctt taagtaaatc ataatcctct aattcttctt
     5821 gaattaatct tttctttgca tctgcttttg aaataagatt atttttaaat ttttgatttt
     5881 ttacttttat aattatagaa aatgcttcgt gctttaattt ttttaaatct tcaaaagtaa
     5941 tctctggtag gtcttttagt cttttatacc aaaaattgtt tagatctttt tgtccttttt
     6001 ttgataaaac tgctaatgtc ttaactccaa ccattcttct taaaatcttt aattttggat
     6061 taacttttct atctatttcg ctttgtatgc ctggcgcatc ttctgcccaa tatcttttct
     6121 cttgaatttt tctaatggtc ggaattactt ttttaaatat tactttgttc ttttcaggta
     6181 atggagttat gggatttcct tttgtgtgta tctttatttt tggttttggc atttttcttc
     6241 actcactttg tatttattct aaaccaaaca tagtaggttc caataactat aataataagg
     6301 aaaagcctaa aaaggaaacc atgcacaaaa tttgcaaaag acaaattgtc gtttattatt
     6361 attagagaag taacaactat tcttgtaaga ttgaaaaata ttatgactgg aattgcaaga
     6421 agcgcgccaa atatcctttt ctttagagaa atacctactg acgagaggat tgcagaaatt
     6481 agaagagaaa actccaagat gcctgaacaa agaaaagaaa tcactatcgg ctcttttaga
     6541 ttagacacaa gaatgcttga agtcttagta agagaatcaa aaacaaaaga attacttgca
     6601 ccaaaaacag ataataacac actagaaaaa aaaccaaaga aataatttat atagccagaa
     6661 actggagact tagcaaacaa caaataagaa agagaaaaaa ctaaaataaa caaaaacaag
     6721 aaaaaaagag ctttcttgtg ctctttctta ttttttaaat cttttttaaa ttttttaaaa
     6781 aaagaattag atttcattta acgaaaacac cagttacttg gcctgactga cctggtcttg
     6841 atgttacttt tacccttgtt tctttaccat ctacatctac tttaaggatt gcgccttttg
     6901 taataatctt ttgtctaaca aagtgcttat ttgcaggatt ctctgtaaca tctataactt
     6961 ttgcttttat agtcttgttg ccttcagaaa ccaaaacaaa ctgagcatga gaaatcttta
     7021 tcttcttatt gccgccaact gttcttgtct taaatctctt atcgacttta gcaactgttg
     7081 tctttgagaa ctttccgcct ttttcgctta aagactttgt tttagcatta actgcgttat
     7141 ttacgccgcc tgttgctttt ctccttgata ctcttttcca ttcaaccata ttttattcac
     7201 gtttttaata attagaaata tattataaat atatgtctta tatatattat tgatatagaa
     7261 aactatttaa aaagaacata caaagagaaa aattatggca aaaccaccaa ttccaagacg
     7321 cgtaataaaa agcagacaga caataccaca atctaaaatt aagccaatac taaaatccaa
     7381 tacagaaaag aaatatgaaa atagagtaag tattgctcat gaaaatattc tagaagtctt
     7441 tgaaaaaatg gattttttaa aagatcttat cgatagcgat cctgaatttg taagacaatt
     7501 tcacgatgct agaaaaaatc taaaaagtct tgggttttct ggaagcattg ataatgcaat
     7561 atatcaacaa ttactttatg caaaggtcgc aagaataata aaaaaagacc cgtattctac
     7621 aagagaaaga attcaaaaga taaacaaaaa aatgaatgaa atttataata atattacaac
     7681 aattgaaagc cttcatgaaa agagaaaacc acatttatca aaggaacaat taacaaaatt
     7741 agaatttgaa aagaatgcaa aaatactaga attaaaaaaa caatattcag agttgcttga
     7801 agaaaaacaa cacataaaaa aattattaaa gcttacaatt aagcaaatag aagaacttga
     7861 aaatattaga taattaatca aacacccttg agaaaagata atgcatcttt tctgctttct
     7921 ttgggcccat cttctctact tcttgtagct ctttaactga tgcttgagaa atgtttctaa
     7981 ttgttttaaa tcttttaaga agagatttgg caagcgttgg gccaatgtct ggaaaggatt
     8041 ctagaataaa aagttgttga tcagaaaaag acatcttatt cttactgaag cgatgtttta
     8101 tttctgtctt tacaccatat tgttctcttt tagcaatcac ataaagaaat aatgctgttt
     8161 ctttttgatt tcgcgtgaaa aatattggaa tacgatagtt aagaccaatt gaagttataa
     8221 gacctatcaa agacttgttg ttgattctgt taccattatc tgagaagttg ataatgcctt
     8281 caataattaa gccaggtttt gacaccttct ctgaaagact atcaagttga gagaaaattc
     8341 gcccatcgat tatcgagctt tcaagatctg ctattgtttt tctttctatc aagacttggt
     8401 cgcttagctg ataatcgcct aaggaaagct gctcgataag cacttcaact tcaaaagaat
     8461 cgttagaaag gttttttaat tcctgattga tttggctaac tctttctctt gtgtcacata
     8521 caatctttag tttttgaggc atat
//
LOCUS       DAOOON010000028         8512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_10284_length_8512_cov_16.963107, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000028 DAOOON010000000
VERSION     DAOOON010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10284_length_8512_cov_16.963107"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..129)
                     /locus_tag="PLK55_03600"
     tRNA            complement(join(3..38,93..129))
                     /locus_tag="PLK55_03600"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Tyr,seq:gta)
     gene            complement(184..1005)
                     /locus_tag="PLK55_03605"
     CDS             complement(184..1005)
                     /locus_tag="PLK55_03605"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HOZ36042.1"
                     /translation="MSFLENFKLKWDISLVSLIFTNLIVIVLAVVQKWDLTVMVFLYF
                     IQGLIIGFFHFLKIINKKRIDVSNIDLTKLPQINYLSFSSIKNLKNLLAFIYWWIYNG
                     FLLQFIFLFFIFRISIIKNAFLGVTSQQIASINWVFFGISILLFFINHLLSYILNFKA
                     DTEKPETIYGLLFLPYVRLIPLFVVVFALFFNYAIILFLVIKTIIDVYTHNYIHTIEN
                     PDNNKKYIINLSKADSAPIILIILVCLFLNYIFPPFIFFFGLAFVKILSMIKRKK"
     gene            complement(1010..1891)
                     /locus_tag="PLK55_03610"
     CDS             complement(1010..1891)
                     /locus_tag="PLK55_03610"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HOZ36043.1"
                     /translation="MNLIDNVKYQFKTDITWISLILVNIITLALAIIQRWDVGTIFYI
                     YLFQTIILWFFTILKLLFKKNISFIGAIDNTGQLISIESKSVAKFQVIIQQITWIFFY
                     GFYFLCFWLLFKPPIFSFGIITVIILFFINHLISFIYYLKKEANKEQLAQTLTKITGY
                     RFVVIHMTIWLTGLFTHFISKTDFSFNLIALIVLFILKTPVDIYFHNKEHETTEDIQK
                     EKNETINKIKQNDAIVKSIKERVISPEEKRRNVILTIFLFVFFIFPIFLGIIGLLIWF
                     IYFLIYLAIMIKPIKIK"
     gene            complement(1904..2332)
                     /locus_tag="PLK55_03615"
     CDS             complement(1904..2332)
                     /locus_tag="PLK55_03615"
                     /inference="COORDINATES: protein motif:HMM:NF022325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2666 family protein"
                     /protein_id="HOZ36044.1"
                     /translation="MDESIQFIANFDKFVSIKKLTIDKEVGPKEVIEFLTSVEFSTSQ
                     KIKDYLAKMCDIKKFEEKTKSLMALEAGKFIDEVNSNNTKKIIKEFIPEGMDKKDVKD
                     AYTELFKVWLIEKYAVEKGIVFAAHHIVFPANKKLMKIKK"
     gene            complement(2396..3103)
                     /locus_tag="PLK55_03620"
     CDS             complement(2396..3103)
                     /locus_tag="PLK55_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36045.1"
                     /translation="MPIKPKLKRRWGKPNKLIESTPEISKKEAFFIKSGKEIKEISKN
                     RAENAVGPDLEKYNAFELKHKGKHIIRIHTHGPGIRHMPSPADIAVFFMDFFNNHLME
                     KNKTIQDSVTKQDLKNSKSEVISVIDEKGKETGRIHVHLSLEAVNQLKEYFLMMSVYT
                     KEQIENKIAWDIQQLTRKQILKKHKKGIELTDDQKYRLHLHATRYYYEKVLKMNVHFK
                     GMPGYKYNEKLCEFEKV"
     gene            complement(3116..3685)
                     /locus_tag="PLK55_03625"
     CDS             complement(3116..3685)
                     /locus_tag="PLK55_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOZ36046.1"
                     /translation="MTGTLITIVGPMFSGKTTELLRIMDRELIAKRHSVLFKPQIDNR
                     YSEEDVLNHNGIGRKAIIANDSHNILDKTLELNKQKAIENVFIDEIQFFDSGIIEIIK
                     KINDLGIAVYTCGLNQIFKGEPFPFKDGKRDIGYLMAISDQIISLDAVCNVCGKKATK
                     TYRIGTDTETVIVGGQDKYQARCKDCFKK"
     gene            3739..4605
                     /locus_tag="PLK55_03630"
     CDS             3739..4605
                     /locus_tag="PLK55_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOZ36047.1"
                     /translation="MERDTILIIEGGAMSGVFAAGVLTAFYKKNLYPRIHSIYAVSAG
                     AHNTAYFISKQVTFASEVYTKELVSKRAKFLNMDVFEMLHKVKNLVLHKKSLQLIDLE
                     VLKEIERIKHKLDLNEIKRSKINFYVKVYDTNQLKPLILNAKENTLDIIEVSSYLAPY
                     ISLKTKYNNLYDGEEIPNDKFLKIVKENKDKKIIWIMNEKKTLAHKIRNSPFTLLNLF
                     TKSLYFGVPFFIHYVYHVFEDPTIEDVQKYSNVIVIYPEGNVRRLTKFTSNKKNLIKL
                     YQEGLLKGKRAI"
     gene            complement(4891..5637)
                     /locus_tag="PLK55_03635"
     CDS             complement(4891..5637)
                     /locus_tag="PLK55_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36048.1"
                     /translation="MIDFKKSIFYGLTGIKWYYLLFIFVLIIIYSIANALQNTTISLI
                     FLSLLFGIIGGVIGTKISGKLKNLKTSSKSILKNSGVYLLMSLIYLIIEVVVIVLGVF
                     LISVAATANIGELTDLSIESIIYSSSTGTLVTIMFLFFLFGFAIMALEFMKVIGLVRS
                     FKTNKFSENFKMLKNLKAIFTKDYFTITMFCLGYGILGIVVVGLLWIILSLFNETAAT
                     IVANILATFLLYVITSSNYSLIADYQHDKK"
     gene            complement(5653..5895)
                     /locus_tag="PLK55_03640"
     CDS             complement(5653..5895)
                     /locus_tag="PLK55_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36049.1"
                     /translation="MPVTKPAGKTAQSGTLKRIIANRNLKKLELRTERDYIKLKAKLQ
                     RLQKPTPKLQEEIDAFHKKMSALPPEIRKKIIRSKK"
     gene            complement(6063..6134)
                     /locus_tag="PLK55_03645"
     tRNA            complement(6063..6134)
                     /locus_tag="PLK55_03645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6100..6102),aa:Ala,seq:tgc)
     gene            complement(6266..6337)
                     /locus_tag="PLK55_03650"
     tRNA            complement(6266..6337)
                     /locus_tag="PLK55_03650"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6303..6305),aa:Ala,seq:agc)
     gene            complement(6710..8204)
                     /locus_tag="PLK55_03655"
     rRNA            complement(6710..8204)
                     /locus_tag="PLK55_03655"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
BASE COUNT         2852 a         1464 c         1187 g         3009 t
ORIGIN      
        1 attccctcct cccagattcg gactggggat ctaccggtca ctgccttttt ctgtcgtttt
       61 acaacaacaa aaatagtgtg tttacttaaa gtctacagcc ggtcgcacta accgctatgc
      121 taaggaggga ttatccttat tttgcttgat ataatattta agaaagaaaa attatataaa
      181 tagctacttt ttgcgtttta tcatagataa tatctttaca aaagcaagcc caaaaaaaaa
      241 tataaatgga ggaaaaatat aattaagaaa caaacaaact aatataatca atataattgg
      301 agcggaatcg gcttttgaca aattaattat atatttttta ttattatctg gattctctat
      361 tgtatggata taattgtgag tataaacatc aattattgtc tttataacta aaaataaaat
      421 tattgcataa ttaaaaaata acgcaaaaac aacaacaaat aatggtatta atctaacata
      481 tggtaaaaat aataatccat aaattgtttc tggtttttct gtatctgctt taaagtttag
      541 aatataagat agaaggtgat taataaaaaa taacaatata gaaataccaa aaaagaccca
      601 attaatagat gcaatttgtt gactagttac gcctaaaaaa gcattcttga taattgatat
      661 tctgaaaatg aaaaataaaa atataaattg taataaaaaa ccattataaa tccaccaata
      721 tataaaagcc aataaatttt tgagattttt tattgacgaa aaagataggt aattaatctg
      781 gggtaactta gttaaatcta tattagaaac atctattctt tttttgttta taattttaag
      841 gaaatggaaa aagccaataa ttaagccttg tataaaatac aaaaatacca ttactgttaa
      901 gtcccatttc tggacaaccg ctaagacaat aacaattaga tttgtaaaaa taagcgaaac
      961 aagggatata tcccatttta acttaaagtt ttctagaaaa ctcataatct cactttattt
     1021 ttattggttt tatcattata gcaagataaa ttaaaaaata aataaaccaa ataagcaaac
     1081 caattattcc caaaaaaatg ggaaaaataa aaaaaacaaa caaaaatatt gttaaaataa
     1141 catttctcct tttttcttct ggactaatta ctctttcttt tattgatttg actattgcgt
     1201 cattttgctt aattttatta atcgtttcat ttttttcttt ttgaatgtct tcagttgttt
     1261 catgttcttt attatggaaa taaatatcta caggagtttt aagtataaat agtacaatta
     1321 atgcaataag gttaaatgag aaatctgttt tactaataaa atgggtaaat aaccccgtta
     1381 accatatagt catatgtata actacaaatc tatatcctgt aattttagta agtgtctgtg
     1441 ctaattgctc tttatttgct tcttttttta aataataaat aaaagaaatt aaatggttaa
     1501 taaaaaataa gattattact gtgattatcc cgaaactaaa aataggcggt ttaaataaaa
     1561 gccaaaaaca aagaaaatag aatccataaa aaaaaatcca ggtaatttgc tgtataatta
     1621 cttgaaattt agcaacactt ttggattcta tagatataag ttgtcctgtg ttatctattg
     1681 caccaataaa gctaatattt ttcttaaata gtagttttaa tatcgtaaaa aaccaaagta
     1741 taatagtttg aaataaataa atataaaata tcgttccaac atcccatctc tgaatgatcg
     1801 ctaatgccaa agtaataata tttactaata ttaaagaaat ccaggtaatg tctgttttaa
     1861 attgatattt tacattatct attaaattca ctttatttac cctctatttc ttaattttca
     1921 ttagtttctt atttgctgga aagactatgt ggtgggctgc aaagactatg cctttttcta
     1981 ctgcatattt ttctataagc catactttaa ataattcagt atatgcgtct ttaacgtctt
     2041 tcttgtccat gccttctgga ataaattctt ttattatctt ttttgtgttg ttggagttaa
     2101 cttcatctat aaatttgcct gcttcaagag ccatcaagga ctttgtcttt tcttcaaatt
     2161 ttttaatgtc gcacatcttt gcaagataat ctttaatctt ttgagaggtt gaaaactcta
     2221 cgcttgtaag aaactctatg acttctttag gtccaacttc tttatctatt gttaatttct
     2281 taatagacac aaatttatca aaatttgcaa taaattgaat tgattcatcc atattttatc
     2341 tttctccttt attttatttt aatatcttaa gtatgaatta tttataaatc taatgttata
     2401 ccttttcaaa ttcacataac ttttcattgt atttatatcc tggcattcct ttaaaatgca
     2461 cattcatttt aagcactttt tcataataat atctggtggc atgtaaatga agcctgtatt
     2521 tttgatcgtc tgttaattct attccttttt tgtgtttttt taaaatttgt tttcttgtta
     2581 attgttgaat atcccaagca attttatttt ctatttgttc ttttgtataa acactcatca
     2641 ttaaaaaata ttcttttaat tgatttactg cttctaaaga tagatgcacg tgaattcttc
     2701 ctgtttcttt gcctttttca tcaattacag aaatcacttc tgattttgaa ttttttaaat
     2761 cttgtttagt tacagaatct tgaatggttt tatttttttc cataagatga ttattaaaaa
     2821 aatccataaa aaataccgca atgtctgcgg ggctaggcat gtgtcttata ccagggccgt
     2881 gtgtatgtat tcttataata tgtttgcctt tatgttttaa ttcaaaagca ttatattttt
     2941 ctaaatctgg gccaactgcg ttttctgctc tatttttaga tatctcttta atttcttttc
     3001 cggatttaat aaagaatgct tcttttttac taatttctgg agttgattct attaatttat
     3061 ttggtttgcc ccaacgtctt tttaatttag gttttattgg cataatttat ctattttatt
     3121 tcttaaaaca atccttacat cttgcttgat atttgtcttg cccgccgaca atcactgttt
     3181 ctgtatctgt tcctatccta taggtctttg tggctttctt tccacaaaca ttacaaacag
     3241 catcaagaga tattatctga tcacttattg ccatcaagta acctatgtct ctttttccgt
     3301 ctttaaatgg aaatggttct cctttgaaga tttggttaag accacaagta taaacagcta
     3361 ttcccaaatc attaattttt ttaattattt ctataattcc tgaatcaaag aactgaattt
     3421 cgtcaatgaa aacgttttct attgcttttt gtttatttag ttctagggtt ttgtccaata
     3481 tattgtgaga atcgttggca ataattgctt ttcttccaat gccgttatgg ttaagtacgt
     3541 cttcttctga gtatctgtta tcaatttgtg gcttgaaaag aacagagtgt cttttagcaa
     3601 ttagttctct atccattatt ctaagtaatt ctgtggtctt accagaaaac attggcccta
     3661 caattgtaat taatgttcca gtcatcatta tactaaataa agaaagcaaa tataaatatt
     3721 attgttatat cttttactat ggaaagagac acaatactaa taatcgaagg aggagcaatg
     3781 agtggagtat ttgctgcagg ggtattgact gccttctata aaaaaaatct ttatcctaga
     3841 atacatagca tatatgctgt ttctgcggga gctcacaaca ctgcttattt tatttcaaag
     3901 caagttacct ttgcttcaga ggtatatact aaagaactag tttcaaaaag agcaaaattt
     3961 ttaaatatgg atgtctttga gatgctccac aaggtaaaaa acctagtatt acataaaaaa
     4021 agtttacagc taattgattt agaagtactt aaagaaattg aaagaataaa gcacaaactt
     4081 gatcttaatg aaataaaaag aagcaagatt aatttttatg ttaaggtata tgacactaat
     4141 caacttaaac ctttaatatt aaatgctaaa gaaaacactc tagatataat tgaagtttct
     4201 tcttatctgg ccccctatat ttctcttaaa acaaaatata ataaccttta tgatggcgaa
     4261 gaaataccta atgacaaatt tctaaaaata gtaaaagaaa ataaagataa aaagataatt
     4321 tggattatga atgagaaaaa aactcttgct cataaaatta gaaacagccc atttacttta
     4381 cttaatctat tcactaaatc tctgtatttt ggcgtaccgt ttttcataca ttatgtttac
     4441 catgtttttg aagaccccac aatagaagat gtacaaaaat attccaatgt tatagtcatt
     4501 tacccagaag gcaatgttag aagattaact aaatttactt ctaacaagaa aaatctaatt
     4561 aaactatatc aagaaggatt attaaaaggt aaaagagcaa tctaagaata aaattatagt
     4621 agttggctat aagccacttt ctgtactaaa ttggcaattc gtctaagcgg aagttgactt
     4681 ccttgcacta gagttatatt ctgcgtcttt cagcctctct ttctgtccat cagttctaca
     4741 catcatttgt taaaacaaaa agccgttata ttctctattc agaaaataac tttaacctag
     4801 tgagtggact ttccttttgg aataaaaatc ccaaatgcca aaacaccaac tactaatata
     4861 taaataaaga gaaaaagaga ataaaaagct ttacttttta tcatgttgat aatctgcaat
     4921 caatgaatag ttagaacttg ttattacata taataaaaat gttgctaata tatttgcgac
     4981 aattgtagcg gctgtttcat taaataaact aagaattatc cataataaac caacaactac
     5041 aattcctagt attccatatc ctaaacaaaa cattgttatt gtaaaataat cttttgtaaa
     5101 tattgctttt aggtttttta acattttaaa attttctgaa aatttatttg ttttaaaact
     5161 tcttactaaa ccaataactt tcataaattc taatgccatt attgcaaaac caaataagaa
     5221 aaacaaaaac attatagtta ctaatgtgcc cgtagatgaa ctatagatta ttgattcgat
     5281 actcaaatct gtaagctctc caatatttgc ggttgctgca acagatatta aaaatacacc
     5341 taaaactatt actactactt ctataatcag atatattaat gacattagta aataaactcc
     5401 tgagtttttc aatattgatt tactagatgt tttaagattt tttaatttcc ctgaaatctt
     5461 tgtgcctata actcctccta ttattccgaa taacaaggat aaaaaaataa gagaaatcgt
     5521 tgtattttgt aatgcattag ctatagagta tattattatt aatacaaaaa taaataacaa
     5581 ataataccac ttaattcctg taagtccgta aaatattgat tttttaaagt ctatcatatt
     5641 atcacaatta atttattttt ttgatctaat tattttcttt cttatttctg gaggtaatgc
     5701 tgacatcttt ttatggaatg catctatttc ctcttgtagt ttaggtgttg gtttttgtaa
     5761 tctctgtaat tttgctttta attttatata atctctttct gttcttaatt ctaatttttt
     5821 taaatttcta tttgcaatta ttctttttaa tgttcctgat tgtgcagttt tacctgctgg
     5881 ttttgttact ggcatactat aatcacacta aataatagaa ataaataatt taaaaagcta
     5941 tctaaaaata gaattgcaga ataaatagtt tcttttccgc gtgtttcttt ttaaaagccc
     6001 aaaggaaaaa ttcttttttt ctgggcccag agaaattcat taatttctct tggaaaaacg
     6061 catggactcc gcgagactcg aactcgcaac cccttccgtg caagggaagt actctaccat
     6121 tgagccagga gccctctaaa aagttttctt aaattgcttg aaactaatat aattaactaa
     6181 agaaataaat ataaataaat ctattttaaa aatttggaag tgttcttttg gaatgaaata
     6241 cttttcttcc aaagaaaagg attcatggac tccgcgagat tcgaactcgc aaccccttcc
     6301 gtgctaggga agtgctctac cgttgagcca gaagccctgt tgtctaaact aatataatta
     6361 attaaagaaa taaatataaa caaatcttaa aattgcgcat aagcgcgaaa actactgcgc
     6421 gccaacccga gcgaaaccgg ttgatggact tcgcgagact cgaactcgca accttctcca
     6481 tgcgaaggag atattctacc attgaactag aagccctcta atataattaa ttaaagaaat
     6541 aaatataaag agttatttta aaatttagtc tgatagaaag aacagaattt acgtttaata
     6601 aaaataaaga ttttaattct ttgtggggtg ttagaagaaa ttaattttgt ttataaaatt
     6661 ttttgtattt ttcaaataat aaatcacttg cgcaatttta ttttaaaata ggaggtgatc
     6721 cgtccgcagg ttcccctacg gacaccttgt tacaacttag tccgtctcgc aaaatgtaga
     6781 attactttaa cctttaatag gctagagttc atccacactc tactcaactg gcttgatggg
     6841 cggtgtgtgc aaggcgcagg gacatattca ccgcgcgata gtgacacgcg attactgggg
     6901 attccagctt catgagggtg agttgcaacc ctcaatccga acttcggcta ggtttctgag
     6961 attgccaccc cctttcgggt tagagactca ttgtcctagt cattgtatgc cgcgtgttgc
     7021 ccagagcaat cgggctatac agacctgcct tcgcccattc cttcctcatt gttaccaacg
     7081 cagtcctatt agagtgcctt tccttatcac taaaaaaagt agcaactaat agcatgggtc
     7141 tcgcccgtta cctgagttaa caggaagctt cacagtacga gctggcgacg gccatacagc
     7201 acctctcagc gcatcaggta agcccttcag actgaccatc atcttgctgt ctgttctggt
     7261 aagatatctg gcgtttcatc caattaaacc gcagcatcca ccccttgtag tgcacccccg
     7321 ccaattccat taagtttctg ccttgcgact gtactcccca ggcggtgaat ttaatggttt
     7381 cccttcggcc ctgagttaac tcgtagttaa cccaacacct aattcacatc gtttacagct
     7441 gggactaacc gggtatctaa tccgggttgc gcccccagct ttcgtgtctc actgtcaaat
     7501 tagttctcgt ttagcgcctt cgccacaggt ggtcttccta gtatcttaag attctactcc
     7561 tactctagga ataccctaaa cgtctcccta tttctagcct accagtatcc tttgccattc
     7621 aatgggttga gcccactgct ttcacaaaag acttaataaa ccagctacac acgctttaag
     7681 ccaaataatg ctggatacca ctcgaggatt gcgtattacc gcggcggctg gcacgcatat
     7741 tgcccccccc ttattcacat agctatctaa gctatataaa agccagtttt agctggcact
     7801 tggagttact ctatcacggt ttcccgcatt gtaaaatttt cgcgactgct gcaccccgta
     7861 gggctaggaa ccttatctca gtttccttct ccgggcaccc tctctcaagg cccgtacaga
     7921 tcttcggctt ggtaggcaat taccctgcca acaacctaat ctgacgcagt cccatccttt
     7981 cgcatcttac aaacatgatc gtaagacctt tgaaaaaaca atcattccag atttgttttt
     8041 ctatcaagca ttatcctcag tttcccaaga ttaacttgtc aaaaaggtag gttgaccacg
     8101 cgttactgag ccgtttgcca aagtctctca tggtcgagac tttcaaactt gcatgtctta
     8161 gtcaaactcc aatagcgata tccaccagca ggatcagctg gaattggttt tattatcgtt
     8221 aatgcacaca aaatctatac acaaagtttt gcctttgcgc acttgtatgt ttctcctaac
     8281 atcagactca aaatagcttg aaccctcaca aaaagaattc tctacaaaaa aacaaagtcg
     8341 aaatttattc tttgtagata taaaaatata agtagagaaa tatttataaa tagatgccta
     8401 aaaattggcg atttaaggta ctctctaact cttaaaatta gagaccatta tactaaaaat
     8461 tattaaaata tagaaagaac aattagttta taaatagatg tttaagtttt ag
//
LOCUS       DAOOON010000029         7988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_11181_length_7988_cov_17.019034, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000029 DAOOON010000000
VERSION     DAOOON010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11181_length_7988_cov_17.019034"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLK55_03660"
     CDS             complement(<1..653)
                     /locus_tag="PLK55_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36050.1"
                     /translation="MTIFKFGFILLIFSILFILNPVYSEYLGNLEFDIQDDGIAIISG
                     NTDYSEFNITQTDKLTSKNKENWLFELNTPIFEKYYYSIILPKDAQINYINSPNSVRI
                     TSTNKRINIISTGSNQNIQIKIQYRIIQNDDLNSYFLEGIISGIVIVFLIIILSFFKY
                     KKKKKELKSKKTNNIFLDELNDRQKKIIDILKSTKNVTQNYLLKELKIPKSSLSRNL"
     gene            complement(654..1523)
                     /locus_tag="PLK55_03665"
     CDS             complement(654..1523)
                     /locus_tag="PLK55_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOZ36051.1"
                     /translation="MFKIKKKWFHKSEHEINKLIIFLGAFLLTITAIVMAHDKNWISV
                     NEGVITTLKTVSIVLWTYFIATIFIRLTQAQVFKLFDESVDAEQKLLLSKLYISFIYV
                     LSTAFVLWRMGVTPENITIFLGLVATGIAFAIRDFILSYFVWFMLLTKKPFRMGDYIS
                     IGEDEGIVKHIGTFFVFIDPINTDLKGTVKIPNKLFLEKKVITYGGVNIPVMVKAPVT
                     SVELKDVEEKLKEIKKNLLKKYPKYTINPKLISDKEYVYLYYDFTVPQTQNLAEIRHK
                     CYEETYKYFKGAR"
     gene            complement(1572..1733)
                     /locus_tag="PLK55_03670"
     CDS             complement(1572..1733)
                     /locus_tag="PLK55_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36052.1"
                     /translation="MSSESRVSPYFSKEQIKLIDGLVNKIGCSRADVVKQLVLIKLTE
                     MGYFNKEKK"
     gene            1861..2784
                     /locus_tag="PLK55_03675"
     CDS             1861..2784
                     /locus_tag="PLK55_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOZ36053.1"
                     /translation="MKRNIEVIERKGIGHPDTIADNLSERLSEELFKEYKKVYGKPKH
                     YNVDKTMVCAGEIHKRKFTNPVKIVFAGQYTKLKNINVILEKVIKDILSFEIEKGMKY
                     EVYNFLNHGSPDLNDIFACKKANDTSFAVGLPESKNEKLIKDIGNYIDYLHKTDKHIG
                     TDNKVMFAFDGKEKNIYIALAFIDNFKSKAEYFKYKAQLEEKLKKKYKIKNLQINIGD
                     TKETQFLTKTGTSLEMGDSGATGRGNRRNGLITPTRPMTLEAYYGKNKITHIGRTYQD
                     LALTMAKKKKRDILLLNKIGGDIKKPIVFEI"
     gene            complement(2791..4290)
                     /locus_tag="PLK55_03680"
     CDS             complement(2791..4290)
                     /locus_tag="PLK55_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOZ36054.1"
                     /translation="MTLNFIQTPKQQKTDFRYSVLDGIFWAIMYYLTSVFLTPYLLEL
                     GANSFEVGLLSYAPLLLAAIVCFFSYDILKLFNSKKQMIVFFVMMHAALWIPVGFSHL
                     FFEGKMQVWFVLIIAFLITIISQFIGPVYTDWIGKLFDLNKIGNYSARKSVILQLISI
                     IPLILAGYLLQAISNRNSLLGFTIIFIFSGFCRFVSGLYINTMSRTEDKEEILKETVH
                     TKKPMFSVFKEEMIKDKTYLYFMIVILIYYFSLYITVPYNKFYLLEIIKLSYTKYLTL
                     EITAIIGVILSLFYWGKACDKFGATKVLKATMIFLPLYPLALILVPNNLAILGFLIFI
                     DAFITAGLGLSITTYIYQNLQKNLITHYTFFMVFQAAALTVGALLGALINKIATNYYG
                     TETKGLVVVFAIGVFFRIITAIFTSKIKDKRPPLRKDKNIVIEIITFRPIRYGIVALT
                     KRFEVNQRRITRKVGQTTVHLHKDLNKTAKRIIKNTKKAITETKKILEK"
     gene            4399..5019
                     /locus_tag="PLK55_03685"
     CDS             4399..5019
                     /locus_tag="PLK55_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOZ36055.1"
                     /translation="MIPIEDKITIDKETLKAIASDTRLDIIKQLDRKKQTLSDLSKSL
                     KLSGPTIKEHLEVLANAGLVKKEESFRKWKYYSLTFKGRKLLRPNETKLFLALIVSLF
                     VGLGIFVFLGLFSGMGSVVSRNSTQLNSADMVTTSSVDNPVIISGEYLETQIADTTTP
                     KQKIIEEPINVIQKPLFNSGYFVLAIVILIIFLIFVLYINVKQKYK"
     gene            5095..6048
                     /locus_tag="PLK55_03690"
     CDS             5095..6048
                     /locus_tag="PLK55_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOZ36056.1"
                     /translation="MKTLIFYHNDIDGLTSALGFAYNEYLLNHNNNIPSYEDLRKDYF
                     FYEVHYGMKNIFEFFSNNGFDWKRFNRVVVLDFCFKDNVMVQLLEKYKEDFIWFDHHK
                     STIEELAKLDIAGLRDDQVATSTIVWRYFNKNPCLFSRYIEDMDIWKWQLPKSNEILQ
                     YIDFLYMQIYDKDKPEKEFEIINLFLKYFNDEYFNSQFTDIIKHGKLIDIYIKTKVKD
                     DIITGKKTIFEGHNAFVLNSQFKPGFISDYIFNNKEYQDIELVVVWYKIYAKSGDKRN
                     FDKVSLRSRTIDCSEIARKFDGNGHPKASGFIISDIDDIIQ"
     gene            complement(6052..6546)
                     /locus_tag="PLK55_03695"
     CDS             complement(6052..6546)
                     /locus_tag="PLK55_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36057.1"
                     /translation="MTELKIKYIFLGNKPIKEPQLINNILCIYSNSGNQFSFAKKNAR
                     LFEYLLNSNNQKNTIALLHNNQFYDFVSKMSNINNYTKILENNKLSRFNYSKDLIRYF
                     QRGDVFAAENLIKKEIKINYLNLLKEKYPQLITQKKENKKNTSKKETKVKQKPLIRKI
                     SISK"
     gene            6626..>7988
                     /locus_tag="PLK55_03700"
     CDS             6626..>7988
                     /locus_tag="PLK55_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOZ36058.1"
                     /translation="MTNNYDIIIIGAGPAGIFSAYELTKSDSTKILKILLIDKGQLVE
                     NRQKTEVMTGFGGAGTFSDGKLHFTPVLSHEKMFHLYSILEYQEYLDYVEKLFIDFGV
                     PQEYYPKDLKKTQEVVDIAQKNNIKLFIRKTIHVGTDKLPAVIQKIEDHLKKNNIDLL
                     PNTEVVDLIVQDNVIFGVKLIDGTELKAKYVICAPGRYNSSWVEKLSQKYNLEKLYDK
                     IEVGVRVEFPAALIEKHAELMYETVYLMYSKTYDDVVRTFCPCPHGFVATEEYDDFVC
                     VNGYSNHGLDGKNSNFALVTEVKLTEPLENTRLYANFVAQGATLVGGGKPIVQRFKDL
                     QAGRRSTYTRLQHAHISPSLKEAIPGDIGLALPYRITKNLIEGIEMLDKILPGLNGDS
                     TFLYAPEVKFRSTKIKTSKHLETQIKNLFMVGDGAGVSGNIVGAAITGVIAAQGIIEK
                     EKQK"
BASE COUNT         2921 a         1030 c         1200 g         2837 t
ORIGIN      
        1 ttaagatttc tagaaagact agatttagga attttaagtt cttttaaaag atagttttga
       61 gttacatttt ttgtagattt taaaatatca ataatttttt tttgtctgtc atttaattca
      121 tctaagaaaa tattgtttgt ttttttagat tttagctctt tcttcttttt tttgtattta
      181 aaaaacgata atataattat caaaaaaacg ataactatgc cagaaattat gccttctaaa
      241 aaataactat ttaaatcatc attttgtata attctgtatt gaatttttat ttgtatgttt
      301 tgattactgc cagtagaaat aatatttatt cttttatttg tagatgtaat tcttacagaa
      361 tttgggctgt tgatataatt aatttgtgca tctttaggta atattataga ataataatat
      421 ttttcaaaaa taggggtatt taattcaaat aaccaatttt ctttgttttt actagttaat
      481 ttgtctgttt gagtgatatt aaattcagaa taatctgtat tccctgaaat tattgcgatt
      541 ccatcatctt gaatatcaaa ttctaaattc cctagatatt ctgaataaac cggatttaaa
      601 ataaataaaa tagaaaatat taaaagaata aaaccaaatt tgaaaattgt catttatctt
      661 gcccctttga agtacttata tgtttcttca tagcatttat gtctaatttc tgcaagattt
      721 tgtgtttggg gcacagtaaa gtcataataa agatatacat attccttgtc tgaaataagt
      781 tttggattga ttgtatattt tggatatttc ttcagtaaat ttttctttat ctcctttaat
      841 ttctcttcta catccttaag ttcaacagaa gtgacaggcg ctttcaccat tactggaata
      901 ttaaccccgc cataagttat tacttttttt tctaaaaata gtttatttgg tatctttacg
      961 gtacctttta gatctgtatt aattgggtca ataaaaacaa agaaagttcc tatatgttta
     1021 actatacctt catcttctcc tattgaaata taatcgccca ttctaaatgg tttttttgta
     1081 agaagcataa accaaacaaa ataagataga ataaaatctc taatagcaaa tgcaatacct
     1141 gttgctacaa gtcctaaaaa tatagttata ttttcaggag taacccccat tctccaaaga
     1201 acaaaagcag ttgataaaac ataaataaat gaaatatata atttacttaa caagagtttt
     1261 tgttctgcat caacgctttc atcaaaaagc ttgaaaactt gtgcttgagt taatctaata
     1321 aatatggtag ctataaagta ggtccatagc acaatagata cggtttttaa agttgtaata
     1381 actccttcat taacagatat ccaattttta tcatgtgcca taacaattgc agtaattgtt
     1441 aataagaatg cgcctaaaaa aataattaat ttgtttattt catgttctga tttatgaaac
     1501 catttttttt ttattttaaa catctctaaa tcacttatat atattgtttg aatatgttta
     1561 tatagtttat tttatttttt ttccttattg aaatatccca tttctgtaag cttgattaga
     1621 acaagctgtt taacaacgtc tgctcttgag catccaattt tgtttaccag gccatcaatt
     1681 aattttattt gctcttttga aaaataaggg ctaactcttg attctgaaga cattttttca
     1741 ccaatttatt ttatagtaac tatttaagtt ctataatagt aactaaaact ccaaaatgtt
     1801 tataaattgc tgtgtgctaa ttagttacta ataagtacat ataaaaaagg agaaaaaaat
     1861 atgaaaagaa atattgaagt aatagaaaga aaaggaatag gccaccctga tacaattgca
     1921 gataatcttt cagaaaggtt gtctgaagaa ttatttaagg aatataagaa ggtttacgga
     1981 aaacctaaac actacaatgt tgataaaaca atggtgtgtg ctggtgaaat tcacaaaaga
     2041 aaatttacaa atccagtaaa gattgttttt gctgggcaat atactaagtt aaaaaacatt
     2101 aatgtaattt tagaaaaagt aattaaagac attttatctt ttgaaattga gaaaggcatg
     2161 aaatacgagg tttataactt cttaaatcac ggcagtccag atctaaatga tattttcgct
     2221 tgtaaaaaag caaatgatac aagttttgct gtaggtcttc ctgaaagtaa aaacgagaaa
     2281 ttaattaaag atattggaaa ctatatagat tatcttcata aaacagacaa gcatattgga
     2341 acagacaaca aagtaatgtt tgcttttgat ggtaaagaaa agaacatata catcgcgctt
     2401 gcatttattg ataatttcaa atctaaagca gaatatttta agtataaagc acaacttgaa
     2461 gagaaattaa agaaaaaata taaaataaaa aacctacaaa taaatatagg agacacaaaa
     2521 gaaacgcaat tcttgacaaa gacaggtacc tctctagaga tgggagatag tggggcaaca
     2581 ggcagaggca acagaagaaa cggcctaata actccaacaa gaccaatgac cttagaagca
     2641 tactatggca aaaacaaaat aacgcacatt ggtagaacct atcaagattt ggcattaact
     2701 atggcaaaga aaaagaaaag agatattctc ttgcttaaca agattggtgg agatataaaa
     2761 aagccaattg tttttgagat ttgatttatt tcatttctct aatatttttt ttgtttcagt
     2821 tattgctttt tttgtatttt taattatgcg ttttgcagtt ttgttaaggt ctttatgaag
     2881 atgaacggtt gtttgtccaa cttttcgtgt gattctcctc tgatttactt caaatctttt
     2941 agttaatgca actatgccat aacgtattgg tctaaatgta attatttcta taactatatt
     3001 tttgtctttt cttagtggcg gtctcttgtc ttttattttg cttgtaaata ttgcagtaat
     3061 tattctaaag aaaacgccga ttgcaaaaac cacaacaagc ccttttgttt ctgtgccata
     3121 atagttagtt gcaatcttat ttattaaagc tccaagaagc gcacccacag tcaatgctgc
     3181 tgcttgaaac accataaaaa aggtgtaatg agttattagg tttttttgaa ggttttggta
     3241 tatgtatgtt gtaatggaca gaccaagacc cgctgtaata aaagcatcaa taaaaattaa
     3301 aaagcctaaa atcgcaagat tatttgggac taaaattaat gctaaaggat agagtggtaa
     3361 gaaaatcatt gtagccttaa gtacttttgt tgcaccaaat ttgtcacaag ctttccccca
     3421 gtaaaaaaga cttaatatta caccaattat tgcagtaatt tctaaagtta ggtattttgt
     3481 atagcttagc tttatgattt caagcaaata aaatttattg taaggcaccg taatgtaaag
     3541 gctaaaatag taaataagaa ttacaatcat aaagtagaga taagtcttat cttttatcat
     3601 ctcttcttta aatacagaaa acattggttt ttttgtgtgc acagtttctt ttagtatttc
     3661 ttctttgtct tctgttctac tcattgtgtt aatatataat cctgaaacaa atctacaaaa
     3721 tcctgaaaat ataaaaatta ttgtaaatcc aagtaaacta tttctattac ttattgcttg
     3781 taataaatat ccagcaagta ttaaaggtat gattgatatt aattgtaaaa ttacactttt
     3841 tcgtgcacta tagtttccaa ttttatttaa atcaaataat ttaccaatcc aatcagtata
     3901 tactggcccg ataaactggc taataattgt tattaaaaat gcaataatta aaacaaacca
     3961 aacttgcatt tttccttcaa aaaagagatg cgaaaatccc accggtatcc atagggcagc
     4021 atgcatcatt acaaaaaaaa caatcatctg cttcttagag ttaaataatt ttaagatatc
     4081 ataagaaaag aagcaaacaa ttgccgcaag tagtagtggt gcataagata gtaatccaac
     4141 ctcaaaacta tttgcgccaa gttctaaaag ataaggcgtt aaaaaaacag aagtaaggta
     4201 atacattatt gcccaaaaaa ttccatccag tacagaatat ctaaagtctg ttttttgctg
     4261 ttttggtgtc tgaataaagt ttaaagtcat atgaaggtct atatataata ttacataata
     4321 gattttaaat attattatta gtaattttcc ttagaaaaaa ccaatattta aaaacacaaa
     4381 gtttctaaga tattttatat gataccaata gaagataaga ttacaattga taaagagact
     4441 ttaaaagcca tagcatcaga cactcgttta gatataatta aacaactaga tagaaaaaaa
     4501 caaactcttt ctgatctttc aaaatcctta aaacttagtg gtccaacaat taaagagcat
     4561 ttagaggttt tagcaaatgc tgggttagtt aaaaaagaag aatcttttag aaaatggaaa
     4621 tactattctt taacttttaa aggaagaaag ctattaagac caaacgagac aaaattattt
     4681 ttagctttaa tagtaagtct gtttgttggc cttggtattt ttgtgttttt agggctattt
     4741 tctggaatgg gttcagtggt atctagaaat tcaacacaat taaattctgc agatatggtg
     4801 acgacatctt ctgtagataa cccagttatt atttctggag agtatttaga aacacaaata
     4861 gcagatacaa cgacacctaa acaaaaaata atagaagaac caattaatgt tattcaaaaa
     4921 ccgttattta attctggtta ttttgtttta gcaatagtta ttttaataat atttttaatt
     4981 tttgtattat atataaatgt aaaacaaaaa tacaaataac caacctattt taaatctttt
     5041 ttgcctttag atatttataa attttagaga tataaagtaa agtgaaatag atttatgaaa
     5101 acactaattt tttatcacaa cgatattgat gggttaactt ctgctttagg ttttgcatat
     5161 aatgaatatt tgttaaatca caataacaat attccaagct atgaagatct tagaaaggat
     5221 tatttctttt atgaagtaca ttatggtatg aagaacatat ttgaattttt ttcaaataat
     5281 ggttttgatt ggaaaagatt taatagagtc gtggttttag atttttgttt taaagacaat
     5341 gtaatggttc agcttttaga aaaatacaaa gaagatttta tttggtttga tcaccacaaa
     5401 agcacaatcg aagaacttgc aaaattagat attgcaggac ttagagatga tcaggtggcg
     5461 acttcaacta ttgtttggag atattttaat aaaaatcctt gtcttttttc aagatatatt
     5521 gaagatatgg acatctggaa atggcaattg cctaaatcta atgagatatt acaatatatt
     5581 gattttttat atatgcaaat atatgataaa gacaaaccag aaaaagaatt tgagattata
     5641 aatcttttct taaaatattt taatgatgaa tacttcaatt ctcaatttac agatataatt
     5701 aaacatggaa aattaattga catttatatt aaaacaaaag taaaagacga tataattact
     5761 ggcaaaaaaa caatatttga agggcataat gcgtttgttt taaattcgca gtttaagcct
     5821 ggctttattt ctgattatat atttaataat aaagaatatc aagatattga gttagtcgtt
     5881 gtttggtata agatctatgc aaaatcaggg gataaacgca attttgataa agtaagtcta
     5941 agatccagaa caattgattg ttcagagata gcaagaaaat ttgatggcaa tggtcatcct
     6001 aaagcaagtg gatttattat ttctgatata gacgatatta tacaataaag attattttga
     6061 tatagatatc tttctaatta gtggcttttg ttttactttc gtttcttttt tggatgtgtt
     6121 ttttttattt tctttttttt gggttatcaa ttgtggatat ttttctttaa gtaaattaag
     6181 ataatttatt ttaatttcct ttttgattaa attttctgca gcaaaaacat ctcctctctg
     6241 gaaatatcta attaaatctt ttgaataatt aaatctgctt agtttgttat tttctaaaat
     6301 ttttgtataa ttattaatat ttgacatttt ggaaacaaag tcatagaact ggttgttatg
     6361 taataacgcg atagtatttt tttggttatt actatttaaa agatattcaa aaagacgggc
     6421 atttttctta gcaaaagaaa attgatttcc tgaatttgaa tatatacaga gaatattatt
     6481 tattaattgt ggttctttaa ttggtttatt tcctaaaaat atgtatttta tttttaattc
     6541 agtcattttc atcacacaac taagaattta gtatacaata atatttaaat tgctttgggt
     6601 ttataattat ttatgtgatt atttaatgac taacaattac gatattatta ttattggcgc
     6661 agggcctgcc ggaatttttt ctgcatacga attaacaaaa tctgattcta ctaaaattct
     6721 taaaatatta ttgattgata aggggcagtt agtagaaaac agacaaaaaa cagaagtaat
     6781 gactggtttt ggtggtgcag gaactttttc tgatggtaag cttcatttta ctcctgttct
     6841 aagccatgaa aaaatgtttc atctctattc tatattagaa tatcaagagt atctagatta
     6901 tgttgaaaaa ctatttatag attttggagt gcctcaagaa tattatccaa aagatttaaa
     6961 gaaaacacaa gaggttgtgg atattgctca aaagaacaat attaaacttt ttataaggaa
     7021 aacaatacac gttggaacgg ataagcttcc tgcagttatt caaaagatag aagatcatct
     7081 taagaaaaat aatatagatt tattgccaaa cacagaagtt gtggatttga ttgtccaaga
     7141 taacgtgatt tttggagtta aattgattga tggcacagaa cttaaggcaa aatatgtgat
     7201 ttgtgcacca gggagatata attcttcttg ggttgaaaag ctttcacaaa aatacaatct
     7261 tgagaaactc tatgataaga ttgaagtagg agtaagagtt gaatttcctg ctgcattaat
     7321 tgaaaaacat gcagagttaa tgtacgaaac cgtatactta atgtattcta aaacatatga
     7381 cgatgttgta agaaccttct gtccttgccc tcacgggttt gtagccacag aagaatatga
     7441 tgattttgtt tgtgttaatg gttatagcaa ccacgggtta gatggtaaaa actcaaactt
     7501 tgctttagtc acagaagtta aattaacaga accacttgaa aacacaagac tttatgcaaa
     7561 ctttgtagca caaggtgcaa cattagttgg gggcgggaaa ccaattgttc agagatttaa
     7621 agatttacag gctggcagaa gaagcacata cacaagatta caacatgctc atatctctcc
     7681 ttctttaaaa gaagcaatac ccggagatat tggattggcc ttgccttaca gaataacaaa
     7741 aaacttaatt gaaggaatag agatgcttga taagattctt cctggtttaa atggagactc
     7801 tacttttctt tatgctcctg aagtaaagtt tagatcaaca aagataaaaa caagcaagca
     7861 tcttgaaaca caaatcaaaa atctatttat ggtaggagat ggtgcggggg tttctggtaa
     7921 cattgttggt gcagcaataa ctggagtaat tgcagctcaa ggaattatag aaaaagaaaa
     7981 gcaaaaat
//
LOCUS       DAOOON010000030         7678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_11750_length_7678_cov_12.602256, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000030 DAOOON010000000
VERSION     DAOOON010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11750_length_7678_cov_12.602256"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..804)
                     /locus_tag="PLK55_03705"
     CDS             complement(40..804)
                     /locus_tag="PLK55_03705"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOZ36059.1"
                     /translation="MINIDELKEYKKKINFNLGQTEKNYLHIAILFAISKLFPEKLMF
                     KGGTALMLCYGLDRFSEDLDFNVVDKDLNIDETLNKIKTFLADYDIVIDYKIEDNKFQ
                     DTQVYFYGPLYKNTNNTRCKNTIDFSRRNDYLSNGAVSRINHIYNEFPVFYINTLDIK
                     EILAEKVRAIMTRKKARDVYDLYFLILKKIEIDKDIINKKLAFYNQKFNLKEFITEIE
                     DREKLWETELPQLILNVPGFKEASGEIISSFKKLFL"
     gene            complement(791..1477)
                     /locus_tag="PLK55_03710"
     CDS             complement(791..1477)
                     /locus_tag="PLK55_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36060.1"
                     /translation="MDLSKLTKLPLFRNKDLKLHYGIKYPEVYICRHITKIKTIEKGK
                     YTLHEDPLIYATQIINPSYLSFLSALNFYGYTTQIPLKATVAITKQKKELRQIKFIKI
                     NKRNFFGYKKIRYGDFEIFIAEKEKLLLDCLIYQKYVSISELHKLLKDNLDKDKLINY
                     LNRINNLDLIRRTGYLLEIKGIDIYEEYLSKIKNTKKYILLNRKLKKSKINNAKWKIN
                     INEVLDYDQY"
     gene            2149..4404
                     /locus_tag="PLK55_03715"
     CDS             2149..4404
                     /locus_tag="PLK55_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36061.1"
                     /translation="MKNINVKKVVAGAAALALGVGVLGVAIAGNNNGTAFGSVAKTDV
                     YNTADMSPKVTLVTGTVGDDDAWAQNIANAIASNAKRENPLYASWVPSDGETTTTTGE
                     GKEFKKNEIGDDFADLKLDYKDYPALGDYKVKVNNTKFGTTEIKVKDFLLVSGNVAFD
                     PDEDVKDLTTTIESQQLKYQVTFTPGIKDGADDTGSPDFKFEIMGKEYTLEEWDGTSK
                     ELTLVQNKATTPYSEGDSFTADNYTIEVVNILDSGAENEYEVELRLKDASGTVKSTDI
                     FVAGDEIFSDYLDVTVEISKVYSNRVAVVAGTSAKLTLKDGDLIEDFPESEDELWAVD
                     MEVVDKALISITIYNDDPDVEWKNENALKIGDSASYPFDFAKVKFEGLTEEDSKAVTV
                     EDGYINYTDADDEDHEIFFYDYASWSNGVERYTTRKTIDDKKLYFDFNTTSGKFTIQE
                     TNSDGEYLDHDGTWGTLHEYDIPDNNTIDFNVGLWNNEEELVTYTIYTDSNTSIKKIA
                     VGLKKGATGTINDLDKDHTWEIGDIYDSVDVDNHKTGIMIGDNDDGFATAALKSMFEF
                     IVSDDTEGAFTAHIDAYTGNLVNTSSTKYKAGLVQVTSEDFYDLDQEETDDLRWAYTD
                     WGTKYEVDGGKFTATIPEEQLYAKIFIGAGTVTTGGTEENPYDEYIMTPVTVSDLLKK
                     DSMAATGTLIVVGGHLANTVADGITNTFLAQTGDYIVGKYSNGNIYVAGWTKADTGTA
                     AGELVTTINGW"
     gene            4728..5987
                     /locus_tag="PLK55_03720"
     CDS             4728..5987
                     /locus_tag="PLK55_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ36062.1"
                     /translation="MITKDELKKVILDQRESFEKSENIIQRQVDLKSYINYDSIVVIS
                     GVRRCGKSTLLKIIKTKINPKDYLYCDFSDERLNNLTIADLQLIYEIFLEKYESGKKY
                     FFFDEIQYVNGWEKFVNRLYEKEEAKIFVTGSNSSLLSSEISSVLTGRNKVIELYPFS
                     FKEYITAKKETIDINNLSTKEGIKLNKYFDDYFNIGGFPHVINTNDVQILKEYYTNII
                     YKDIIVRKKIRQVKEIKEIISFLLNNIGQIISYKKIIANSEIKSTSTIKNFIDYLNEA
                     YILFTLSKYDKSIRKQIQNPKKSYFIDMGMVRKISVNFSENNGWYLENVVFLELLRRN
                     KEIYYHKKEKECDFVVKEGTQIKEAIQVSYEMNQKTETREIKGLINAIKTYNLKEGII
                     ITRDQEETRIVEGYKIKIIPIAKWLLE"
     gene            6278..7528
                     /locus_tag="PLK55_03725"
     CDS             6278..7528
                     /locus_tag="PLK55_03725"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ36063.1"
                     /translation="MYSKEQLKEIIVSQKEAIDSQDIGLIRTDYLKLLKESIHPKIVT
                     ILKGPRRVGKSTIVLQFIKEINNNYYYFDFDDERIANFKTGDFETLMLAFQELYQDKD
                     YLLFDEIQNIDSWELFINRQLKQNKKIIITGSNSKLLSADLGTHLTGRHIDIEIFPFS
                     FKEYLYAKDFKTKQAYTIKEKAELEKLFKDYQKTGGFPQVVFFYDYRILQELYRDIIE
                     KDTKDLKSNQKIKDVSDYLLFNISTLVSLRSIQKNFGLKNTNEAKAIIKRLEATYLFQ
                     FIEIYSNSYKERKANPMKCYSIDVGLVNNISTKLVEDRGQQFENLVYLKLRKENAQIY
                     YYKKKGETDFIVIDNKKPILVAQACSNLADNKTKEREVSSLLECMDQLNIKVGIIINK
                     DLEKEETIDGKRIKFIPIINWLLE"
BASE COUNT         2982 a         1029 c         1181 g         2486 t
ORIGIN      
        1 atacataagc taaagagaga aaatgaggct ctttctaaat taaagaaata gttttttaaa
       61 gctagatatg atttcgccac ttgcctcttt aaaacccgga acattaagaa tcaactgagg
      121 taattctgtt tcccagagtt tttctctgtc ttctatttct gtaataaatt cttttaaatt
      181 aaatttttga ttatagaatg ccagtttttt atttattata tccttatcaa tttctatttt
      241 ttttaatatt aagaaatata agtcataaac atctctcgct ttttttctag tcattatggc
      301 ccgcactttt tctgctaata tttctttaat atctaaggtg ttgatataaa atacagggaa
      361 ttcgttatag atgtgattta ttctagaaac tgcgccatta gagaggtagt cgtttcttct
      421 agagaaatca atagtgtttt tgcatctggt gttgtttgta tttttatata atggcccgta
      481 gaaataaact tgtgtgtctt gaaatttatt gtcttctatt ttatagtcaa ttacaatatc
      541 atagtctgca agaaacgttt ttattttatt taatgtttca tcaatattca aatctttatc
      601 aactacatta aaatccagat cttcagaaaa tctatccagt ccataacaaa gcattagtgc
      661 tgttcctcct ttaaacatta atttttcagg aaataattta gatattgcaa aaagtattgc
      721 aatatgcagg tagttttttt cagtttgccc cagattgaaa tttatttttt ttttatattc
      781 ttttaattca tcaatattga tcataatcaa gaacctcatt tatatttatt ttccattttg
      841 cgttgttgat ttttgatttt ttaagttttc tgtttaatag gatgtatttt ttagtgtttt
      901 taatttttga tagatattct tcatagatgt ctattccctt aatttctagt agataccctg
      961 ttcttcttat taaatcaaga ttatttattc tatttaaata attaattagt ttatctttgt
     1021 ctagattgtc ttttaataat ttatgtaatt cagatatact aacatatttt tgatagatta
     1081 aacaatctaa tagtagtttt tctttctctg caataaatat ttcaaaatcg ccatatctta
     1141 tttttttata tccaaaaaaa tttcttttat ttattttaat aaactttatt tgccttagtt
     1201 cttttttttg tttagttata gcaactgttg cttttaacgg tatttgagta gtatagccat
     1261 aaaaattaag cgcagataaa aaagaaagat aagatggatt aataatttgt gttgcataaa
     1321 ttaatggatc ctcgtgcagc gtatatttcc ctttttctat agtttttatt tttgtaatgt
     1381 gtctgcagat atatacttct ggatatttaa tcccatagtg taattttaga tctttgtttc
     1441 taaacaaagg cagtttagtg agttttgata aatccataat aaacatatat ataaaaacat
     1501 ttataaccct ttttatatat taaattatac aaaaaagtac aatttacgtt ataaaaagta
     1561 ggcgacataa aaatagtaaa aaaagatatc gtttttgata tcttttggag tttttttatt
     1621 attttggctt ttatttattt taggtgatca aactcataca cccctgttta gttaaatgtt
     1681 tttgatttta gcaatttaat aaaattagaa aaggtgcagt cttttatttc ataatttttt
     1741 tcttcaagaa ggcgcactct tccaccccct gtgtaattta gtttttattt tttgaaaaat
     1801 gtattttaag ctatataata tataagaaaa aaaatctaag taaaacgcag tttctatttt
     1861 ttttaaaatt cttattttaa ggggccaatt atacataaaa acgggtttaa ttttgccata
     1921 acctcgctat ttctctaata tttcagccac taaaaaagta agctctaaaa accaaccctt
     1981 tttaaacatt actgcccctt aagttctata ttccacatat atggaataac tgaatctgtt
     2041 ttgtactgga gtttattttt gagccagtca ttaatacaca caggttcaat cttcataaac
     2101 aaaaaaagtt tctttgttag aaaagaaact aaaatggagg agaaataaat gaagaatata
     2161 aatgtaaaga aagtagttgc tggcgcagca gcacttgcat taggtgtagg tgttcttggc
     2221 gtagcaatag ctggtaacaa caatggtaca gcatttggtt ctgttgcaaa gacagatgtt
     2281 tacaacacag cagacatgtc accaaaagta accttagtta ccggaacagt cggagacgat
     2341 gatgcatggg cacaaaacat tgcaaacgca attgcaagta atgcaaagag agagaaccca
     2401 ctatatgctt catgggtacc atccgatggc gaaacaacca caacaaccgg cgaaggtaaa
     2461 gaattcaaaa agaacgaaat aggagacgat tttgcggatt taaaactaga ttataaagac
     2521 tatcctgcgc ttggagacta taaagtaaaa gtaaataata caaaatttgg aacaacagag
     2581 ataaaagtaa aagatttctt acttgtttca ggtaatgttg cttttgatcc agacgaagat
     2641 gtaaaagact taacaacaac aatagagtcc caacaattaa aataccaggt aacatttaca
     2701 ccaggaataa aagacggtgc agatgataca ggaagtccag atttcaaatt cgagattatg
     2761 ggtaaagaat atacactaga agaatgggac ggtacatcaa aagaattaac tcttgttcaa
     2821 aataaggcaa ccacaccata tagtgaagga gattctttta cagcagataa ttacacaata
     2881 gaagtagtaa acatccttga ttctggggca gagaatgaat acgaagtaga attaagatta
     2941 aaggatgctt caggcacagt caaatctaca gacatatttg tagcaggaga cgaaatattt
     3001 agtgactatc ttgatgtaac tgtagaaatt tcaaaagtat attctaacag agttgcggtg
     3061 gtcgcaggaa ctagtgcaaa attaactctt aaagacggag atttaattga ggatttccca
     3121 gaatctgaag atgagttatg ggcagtagat atggaggttg ttgataaagc attaataagc
     3181 ataacaatct ataatgatga cccagatgta gaatggaaaa acgaaaacgc tctaaaaatc
     3241 ggagattcag catcatatcc atttgacttt gcaaaagtta agtttgaagg tcttacagaa
     3301 gaggattcaa aagcagtaac tgttgaagat ggatatataa attatacaga tgcagatgac
     3361 gaagatcacg aaatattctt ttacgactat gcttcctgga gtaacggtgt agaaagatat
     3421 accacaagaa aaacaattga tgacaagaaa ctgtactttg atttcaatac aacttctggt
     3481 aaatttacta tacaagaaac aaacagcgac ggggaatatc tagatcacga cggaacttgg
     3541 ggtacattac acgaatacga tataccagat aacaatacaa tcgactttaa tgttggatta
     3601 tggaataatg aagaggaact agtgacctat accatatata ccgattcaaa tacaagcatt
     3661 aaaaaaatcg ctgttggctt aaaaaaagga gctacaggaa ccataaacga tttagacaag
     3721 gaccacactt gggaaattgg tgacatatat gacagcgtag atgttgacaa ccacaaaacc
     3781 ggtataatga tcggtgataa cgatgatggc tttgcaacag cagcactaaa gtctatgttt
     3841 gaatttatcg tttcagatga tacagaaggg gcatttactg cacatatcga cgcatataca
     3901 ggcaatttag taaataccag cagtacaaag tacaaagcag gtcttgttca agttacttct
     3961 gaagacttct atgatttaga tcaagaagaa acagatgatt taagatgggc atataccgat
     4021 tggggcacaa agtacgaagt tgatggtggt aaattcactg caacaatacc agaagagcaa
     4081 ttatatgcaa aaatctttat cggtgcagga acagtaacaa caggcggtac agaagagaac
     4141 ccatatgatg aatacattat gactccagta accgtgtcag atcttctaaa gaaagactct
     4201 atggcagcaa ccggtacctt aatcgttgtc ggtggacacc ttgcaaatac agtagcagac
     4261 ggtataacaa ataccttcct tgcacaaaca ggagattaca ttgtgggtaa atactcaaac
     4321 ggtaatatct acgttgcagg ttggacaaag gcagacacag gtacagcagc aggcgaatta
     4381 gtaaccacaa taaatggttg gtaaataagt ctaaaaccaa aattgtaagc taataactta
     4441 caattttctt ttctttttct ttttgaaccg gttttacgcg gattcacgcg caatatttaa
     4501 aactcgctat cagaccataa tcttacaatc acacaatgca tcattgtaaa tgacacaaaa
     4561 tagaaaaatg tgtcattaca aatgacacat ctaaaagatg tttaaatatc ataatctaaa
     4621 caacaccgcg cgcatgtgtt cgttactatc gaaagctttg cttagtaatc attcgttact
     4681 atcgaaagct ttaaatatta tttcaaccac tatatttatg attgtgtatg ataacaaaag
     4741 atgaattaaa aaaagtaatt ttagatcaaa gagaaagttt tgagaaatca gaaaacatta
     4801 tacaaagaca agtagattta aaatcttata taaattatga ttcgattgtg gtaataagtg
     4861 gggtaagaag gtgtggtaaa tctacattat taaaaataat taaaactaaa ataaatccaa
     4921 aagattacct ttattgtgat ttttcagacg aaagattaaa taatttaaca attgcggatt
     4981 tacaactaat atatgaaata tttttagaaa aatacgaatc tggaaaaaaa tatttttttt
     5041 ttgatgaaat acaatatgta aacgggtggg aaaaattcgt aaatagatta tacgaaaaag
     5101 aagaggcaaa aatatttgta actggatcaa acagctcttt acttagttca gaaatatcgt
     5161 ctgttttaac aggaagaaac aaagtaatag agctttaccc attttctttt aaagaataca
     5221 ttactgcaaa gaaagaaaca atagatataa acaacttatc aacaaaagaa ggcattaaat
     5281 taaataaata ttttgatgat tattttaata taggtggatt tccgcatgtt ataaatacaa
     5341 atgatgttca gatattaaaa gaatattaca caaatataat atataaagac ataattgtta
     5401 gaaaaaagat aaggcaagta aaagaaataa aagaaataat ctcattttta ttaaataaca
     5461 taggccagat aatatcatat aaaaagataa ttgctaattc agaaataaaa agcacaagca
     5521 caataaagaa tttcatagat tatcttaatg aagcatatat tttatttaca ttatccaaat
     5581 atgacaaatc tatcagaaaa caaatacaaa atccaaaaaa atcatatttt atagatatgg
     5641 ggatggtaag aaaaataagt gttaattttt cagaaaataa tggctggtat ttagaaaacg
     5701 ttgtattttt agaattatta agaagaaata aagaaattta ttatcataaa aaagaaaaag
     5761 aatgtgattt tgtagtaaag gaaggcacac aaattaaaga agccatacaa gtatcttatg
     5821 aaatgaatca aaaaacagaa acaagagaga ttaaaggatt aattaatgca ataaaaacct
     5881 ataatcttaa agaaggaata attataacga gagaccaaga agaaacgaga atagtagaag
     5941 gatataaaat aaaaataatt ccaatagcta aatggttatt agagtaattt cttttctttt
     6001 tatttagttt aaaacttgct atcagactaa ataaatgtgc tatttttgtc aattgttatt
     6061 gttcatatga ttgaccaaaa aagacgtttt ttgttcagcc aactgaccat aattttttat
     6121 tacaaatgcg gctcatttat atctagaaaa atgttcttta atatcgaata cttttgtctt
     6181 taaagaaaaa agtgcgtgcg tttctccacc cactttcgtt tttaagtgcg tgcgttttac
     6241 cactcgcttt ttaaatgttg ttatacatta attatttatg tattcaaaag aacaattaaa
     6301 agaaataatt gtttctcaaa aagaagcgat tgattctcaa gatataggac taataagaac
     6361 agattattta aaattattaa aagaatcaat tcatccaaaa atagtcacaa tattaaaggg
     6421 cccaagaaga gtaggaaaat caacaatagt tttacaattt ataaaagaga taaataacaa
     6481 ctattattat tttgattttg atgatgagag aatagcaaat tttaaaacag gggattttga
     6541 aacactaatg ctggcttttc aagagctata ccaagataaa gattatttgt tatttgatga
     6601 aattcaaaat atagattctt gggaattatt tataaatagg caattaaaac aaaataaaaa
     6661 aataataata actggctcaa actcaaaatt attaagtgct gatttaggca cacatctaac
     6721 tggcaggcac atagacatag aaatattccc tttttctttt aaagaatacc tttatgcgaa
     6781 agattttaaa actaaacaag catacactat aaaggaaaaa gcagaacttg aaaaattatt
     6841 taaagattat caaaaaaccg gaggtttccc acaagttgta tttttttatg actataggat
     6901 attacaagaa ctatacagag acataattga aaaagacaca aaagatctaa aaagtaatca
     6961 aaaaataaaa gacgtttccg attatttatt atttaatatt tctacattag tttctttaag
     7021 aagcatacag aaaaactttg gtttaaaaaa cacaaacgaa gcaaaagcta taataaaaag
     7081 actagaagca acatatcttt tccaatttat agaaatttat tcaaattctt ataaggaaag
     7141 aaaagcaaac cccatgaaat gttattcaat agatgttggt ttagtaaaca atatttcaac
     7201 aaaactagta gaggataggg ggcagcaatt tgaaaattta gtatatttaa agctaaggaa
     7261 agaaaatgca caaatatatt attataaaaa gaaaggggaa acagatttta tagtaataga
     7321 taataaaaaa ccaatacttg tagcacaagc ttgtagtaat ctagcagaca ataaaaccaa
     7381 agaaagagaa gtaagttctt tattagaatg tatggatcaa ttaaatatta aagtcgggat
     7441 aataataaac aaagatctag aaaaagaaga aacaattgat ggcaaaagaa taaagtttat
     7501 acctataatt aattggttat tagaataact acttggttaa taacttacaa tttctttttt
     7561 taatttctaa aatctcattc ttgctatcag acaagcattt tttgtacttt ttttagtttt
     7621 taacaattag ttaattattt aaatataatt gtctataaat atattatatg caaacaaa
//
LOCUS       DAOOON010000031         7432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_12258_length_7432_cov_16.022502, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000031 DAOOON010000000
VERSION     DAOOON010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12258_length_7432_cov_16.022502"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLK55_03730"
     CDS             complement(<1..511)
                     /locus_tag="PLK55_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36064.1"
                     /translation="MFRLKSRKGISEIISVTLLIVIVVILASLFFAWSRNSAKTKMDQ
                     VTAELKAASDLECSNAQFYVDSCTIYSTTKTVRLVLMNNSDIRLFNLQLIINGNVSGS
                     FNKIVEAGETAYLSTDTNFTVNRGDQNSLVDIDIEDIDNITLTNGACPKEVIDVSGCN
                     IVSTFWPPTA"
     gene            complement(511..1752)
                     /locus_tag="PLK55_03735"
     CDS             complement(511..1752)
                     /locus_tag="PLK55_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOZ36065.1"
                     /translation="MVNTLLFLLLMSGIFLLGFFGNIFFKKTKISDILILIFVGMLLG
                     PIFHLIPINIVELLKSFTPIFAAVALIILLFDGGLCLNFNKVVNEIKNSFIFTILIFL
                     FTAGISGVFLHYVFGVEWIFGFLAGSIIGGTSSAIVIPLVTRSSAGEKTRIILTLESA
                     MTDVFCVVATITIAGLIVSQSINATNIAQGIFSAFAIATVIGLVGGLIWIKVLRDYTE
                     TKDFDYLLTLAFLFLLYVATEYLNGNGAFCALIFGLVLGNASTILKAFKMKEFTMEKS
                     MSHFQTEISLLIKTFFFVYLGIIIDLTIISLETILISLGLIVVLIIVRILAAKLLFSK
                     SLNKTDQNIISALHARGLAAAVLATYPISIGIINDFTNKLVAVVFLVILITNITTTIY
                     LFLVEKGIAKEFKIKANNLVK"
     gene            1858..2568
                     /locus_tag="PLK55_03740"
     CDS             1858..2568
                     /locus_tag="PLK55_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOZ36066.1"
                     /translation="MVEKKHNANLNKKVISTVTQIVNGMANKKKGSFFIISKSDISNK
                     YERLYPDLFSNKKVSIFKKETQTLLIALLELDGAIVVDNSGYIVDYGVKVKKTKILLG
                     HGTRHSAARGISCFPNVLSIISSEEDGKVRLFRNGEMVAEINPKMGKSKLFFERVGEL
                     FSKPELQVATAGGVASLLLRVNPWFAGIIFTGSWIVTKYGLVSLQDFLRTGHLVVKKQ
                     TKEIKKQIKKNRNARAKK"
     gene            2549..2992
                     /locus_tag="PLK55_03745"
     CDS             2549..2992
                     /locus_tag="PLK55_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36067.1"
                     /translation="MQEPKNKVTEVSSGIINSFIRITKKTIIFGIIIPGVIYLIIRFL
                     EIAFGYSWQDQLIFQYISYIYGIGYIAFLTYKAVAGQIHTTIYYSIGWILGLLLLFRF
                     GVFNLQDFLNILLIPTIFVILKIIYLIFRKPTKIIRKESIRRRSP"
     gene            complement(2973..5237)
                     /locus_tag="PLK55_03750"
     CDS             complement(2973..5237)
                     /locus_tag="PLK55_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36068.1"
                     /translation="MKKIKKATVSLTKKEIIEKLNKQLNSPKKFIISPIDISLLRKKK
                     ISINRYAKDLQKVKANYTIFLGLKYTLKDLKNAGFNNLELKKLGFSLNEPKKVKETIP
                     QKPVLKEKKATASLNKKEIIEKLNKQLNSPKKFIISPIDISLLRKKKISIIRFAKDLQ
                     KAKANRTIFSGLKYTFTDLKKAGFTAAEIKTLGFKEEKKEKPQKITIKKPIKKKKITL
                     SELLRKKTPAKKLLQLGYNHKRLKKAKYTATRLKKMKFSSMYLFTLGFSAQELRNAKY
                     TLTELKRAGYSAKELKDTGYSAKDLLKAKFSEDVVAYAQYPAKDLYKLNIPVKKLKIA
                     GYKANELIEAGYTLEDLKKAYTVSELLLAGYTLLDLVDKEKINIQDLLDEGITVSDLL
                     KEGFRIKELVPLGVTLKQLREAGISARDMYRAGYSVRDLKEAGYKLIEIFELRDLLTN
                     QEKKEEFSLEDLRYLNYSAKDLLLFGYSPAELYFSGFTTKELSQAGLSCSQLMDAGCS
                     LSEIRLCGCELPEFIAKGYSAKELKKAGFSALEIHLAGYSLYDLRDAEFDAKELLGIG
                     YSANQFHNLGFTARELYFAGFDLKVIKDAGYSAKELADLDIDVIEFAKAGYSAKEMRA
                     ASYSAYDLKKAYSAKELRYAGFDSASLRDAGYSAKELHDVGFYPRELYYAGFPAKDVW
                     KLGLKARQLRVIRYSAKELKPLGFSAKELYKAGYSPDQLIKAGYGYKELAELGISQRR
                     LQRHVRINSKGYVF"
     gene            5419..5769
                     /locus_tag="PLK55_03755"
     CDS             5419..5769
                     /locus_tag="PLK55_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36069.1"
                     /translation="MAKKETKIGAYIFIAGIVIAIISGILMNFISPVVITSLLMILGL
                     IVGFLNITDKEVKDYLLTAVCLVIVTSLGGASIQNLMIIGPYLAGVLHAIMIFTIPAT
                     IVVALKEIISIAKN"
     gene            complement(5822..>7432)
                     /gene="glmS"
                     /locus_tag="PLK55_03760"
     CDS             complement(5822..>7432)
                     /gene="glmS"
                     /locus_tag="PLK55_03760"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOZ36070.1"
                     /translation="WATHGEPTVINAHPHTDCKTEIALVHNGIIENFETLKKFLEKEG
                     HVFKSETDTEVLAHLIEKFYSGDLKKAVQKALKKVVGTFGIVVIDKQGKELIVARRGS
                     PIVIGIGDCEYFVASDVSAIIEHTKKVMYLEDNEMACINGGCEITSLDNITLDKETIE
                     IETNLEEIEKQGYKHFMLKEIHEQPETIKQAILGRIDFENNTAKLGGINLTTEQIKNI
                     KRIIIVACGTSWHCGLVAKFSMEGFLRIPVEVDYASEFRYRNPIIDNNTLIIAISQSG
                     ETADTLAAVREAKKKGAKVIGIVNVVGSTIARECDGGIYIRAGPEIGVASTKAFTSQL
                     VCLNLLTLYLGRIIGSIDDSGAKKLINNLLELPHIIRAELKKENEEKIKEIAKKYYIC
                     KNTLYLGRGINYPIALEGALKLKEISYIHAEGYPAAEMKHGPIALIDKGLPVVVIANS
                     DLNYDKVLSNIHEAKARGASIIAIATQGDEEIEKLCDHVIFVPITCSNLTPFVNVIPM
                     QLLAYYIADFKDCAIDQPRNLAKSVTVE"
BASE COUNT         2445 a         1175 c         1018 g         2794 t
ORIGIN      
        1 ttgctgttgg tggccaaaaa gtgctaacaa tattacaacc gcttacatca ataacttctt
       61 ttgggcaagc gccgtttgtt aaagttatat tatctatatc ttcaatatca atatccacta
      121 aagaattttg atcccctcta ttaacagtaa aatttgtgtc tgttgaaaga tatgcagttt
      181 ctcctgcttc tactatttta ttaaaacttc cagaaacatt tccattaatt attagttgta
      241 aattaaataa cctaatatct gaattattca ttaaaacaag tcttactgtt ttagtagtac
      301 tatagattgt acaagaatca acataaaatt gcgcatttga gcattctaaa tcgcttgctg
      361 cttttagctc tgctgtaact tggtccattt ttgtctttgc cgagtttctt gaccacgcaa
      421 agaaaagact agcaagaatt actacaatta ctattaataa cgttacacta ataatctcag
      481 aaatcccttt tcttgatttt aatctaaaca ttatttcacc aaattatttg ctttaatttt
      541 aaattcttta gctatccctt tttctaccaa aaataaatag atggttgttg taatgtttgt
      601 tattaatatt actaaaaata caaccgccac aagcttattt gtaaagtcat taataattcc
      661 tatacttatg ggatatgttg caagtactgc agctgctagg cctctagcat gaagagcaga
      721 aataatattt tggtctgttt tattaaggct ttttgaaaac aataattttg ctgcaagaat
      781 tctaacaatt attaaaacaa caatcaagcc taacgagatt aaaatcgttt caagagaaat
      841 aattgtaagg tcaattatta ttccaagata cacaaagaaa aaagtcttaa ttaataatga
      901 aatttctgtc tggaaatgag acatgctttt ttccatagta aattctttca tcttaaatgc
      961 ttttagaatt gttgaagcat tacctagaac taatccaaag attaatgcac agaatgctcc
     1021 gtttccattt agatattctg tcgctacata taataaaaat aaaaaagcta aagtaagaag
     1081 ataatcaaag tcttttgttt ctgtatagtc tcttaatact tttatccaga ttaaccctcc
     1141 aactagacca ataacagtag ctatagcaaa tgcagaaaat atgccctgag ctatatttgt
     1201 tgcgtttatt gattgagaaa caattaaccc tgcaattgtt attgttgcga caacacaaaa
     1261 aacatctgtc attgctgatt ctaatgtcag tattattctg gttttttcac ctgcacttga
     1321 tcttgtaact aatggaatta ctattgcaga actagtaccg ccaataattg atcctgcaag
     1381 aaacccaaat atccattcta ctccaaaaac ataatgtaaa aatacaccac taattcctgc
     1441 agtaaataaa aatattaaaa ttgtgaaaat aaatgaattt tttatttcgt tgacaacttt
     1501 attaaaattt aagcacaatc caccatcaaa caataaaata attaatgcaa ctgctgcaaa
     1561 tattggagta aaagatttta atagttcaac aatattaatc ggtattaaat gaaatattgg
     1621 ccctaaaagc ataccaacaa atattaaaat taaaatatca gatatcttgg ttttcttaaa
     1681 aaaaatattt ccaaaaaagc caagtaagaa tattccggac atcaataaca aaaacagaag
     1741 tgtatttacc ataatttatc tctaattttt tatttttaat aaaatatatg ttagtaaatt
     1801 attttataaa cttaactaaa aatatataat atagggcaat taggagaaat agataatatg
     1861 gtcgaaaaaa aacataatgc aaacttaaac aaaaaagtta tttctacagt tacacaaatt
     1921 gtaaacggaa tggcaaacaa gaaaaaaggt tcttttttta taataagtaa atctgatatt
     1981 tctaataaat atgaaagact ttatcctgat cttttttcaa ataagaaagt aagtattttt
     2041 aaaaaagaaa cacaaacact tctgatagct cttttagaac ttgatggtgc aattgttgtg
     2101 gataattctg gttatatagt agattatggc gtaaaggtta aaaaaacaaa aatacttttg
     2161 ggacatggaa caagacattc tgctgctaga gggatttctt gttttcctaa tgttttatca
     2221 attatttctt cagaagaaga tggaaaggtt agattattta ggaatggaga aatggttgca
     2281 gagattaatc caaaaatggg taaatccaaa ctgttctttg aaagagtagg agaattattt
     2341 tctaaaccag aattacaagt agcaactgct ggcggagttg cttctttact tttaagagta
     2401 aatccttggt ttgcaggtat tatctttact ggttcatgga tagttacaaa atatgggcta
     2461 gtaagtctac aggattttct aagaacaggg catttggttg ttaagaaaca aacaaaagaa
     2521 ataaaaaaac aaattaaaaa gaatagaaat gcaagagcca aaaaataaag ttacagaagt
     2581 atcttctgga attattaact cttttattag aattacaaaa aaaacaataa tttttggaat
     2641 tattattccc ggagtaattt atttaattat tagattttta gaaattgctt ttggttattc
     2701 ctggcaagat caactgattt tccaatatat ttcttatatt tatggtattg gttatattgc
     2761 gtttttaact tacaaagcag ttgctggtca aattcacaca acgatttatt attcaattgg
     2821 ttggatttta gggttattat tgttatttag atttggagta tttaatttac aggactttct
     2881 aaacatcttg cttattccaa ctatatttgt aattctaaaa ataatatatt tgatatttag
     2941 aaaaccaaca aaaataataa gaaaagaaag tattagaaga cgtagccctt agaatttatt
     3001 cttacgtgtc tttgtagtct tctttggcta attcctagtt ctgctaattc tttataacca
     3061 tagccggctt ttattagttg gtctgggctg tagcctgcct tatatagttc ttttgcagaa
     3121 aagccaagag gttttagttc ctttgcagaa tatcttataa ctcttaattg tctagctttt
     3181 agacctaatt tccaaacatc ttttgcagga aagccagcat aatatagttc tcgtggataa
     3241 aagccaacat catgtaactc ttttgctgaa tatccagcat cccttaaaga agcagaatca
     3301 aagcctgcat atctaagctc tttagcagaa tatgctttct ttaaatcata ggcactatag
     3361 cttgctgctc tcatttcttt agcagaataa ccggcctttg caaactctat aacatcgata
     3421 tcaagatcag ctagttcttt tgcagaataa cctgcatctt ttatgacctt tagatcaaaa
     3481 cctgcaaaat aaagctctct tgcagtaaaa ccaagattat gaaattgatt tgcagaataa
     3541 ccaataccaa gaagttcttt agcatcaaac tctgcatctc ttaaatcata taaactatag
     3601 cccgccaaat gaatctcaag agcagaaaaa ccagctttct ttagttcttt tgcagagtaa
     3661 cctttcgcaa taaactctgg cagctcacaa ccgcaaagtc ttatttcaga taaagaacaa
     3721 ccagcatcca ttaattgtga acaagaaaga cctgcttgag ataattcttt tgtagtaaaa
     3781 ccagagaaat ataattctgc aggagaatat ccaaacaaca gtaaatcttt agcagaataa
     3841 ttaagatatc ttaaatcctc taaactaaat tcttcttttt tctcttgatt tgttaaaaga
     3901 tctcttagtt caaagatttc tataagctta tagcctgctt cttttaaatc tcttacagaa
     3961 taaccagctc tatacatgtc tcttgcagag attcctgctt ctcttaattg ttttaaggtt
     4021 acacctaatg gtactaattc tttaattcta aagccttctt ttaaaagatc agaaactgtt
     4081 atgccttcat caagtaaatc ttgaatattt atcttttctt tgtctactaa atctagaagg
     4141 gtgtagcctg caagtaatag ttcactaact gtgtatgctt tttttaggtc ttcaagagta
     4201 taacctgctt caattaattc atttgcttta tagcctgcaa tctttaattt ctttacagga
     4261 atatttagct tatataggtc ttttgcagga tattgtgcat aagcaaccac gtcttcgctg
     4321 aatttagctt ttagtaaatc ttttgctgaa taacctgtgt cttttagttc tttagctgaa
     4381 taacctgctc ttttaagttc tgttaaagta tattttgcat ttcttaactc ttgagcagaa
     4441 aaccctaaag taaaaagata cattgaacta aatttcattt tctttagtct tgttgcagtg
     4501 tattttgctt tttttagtct tttatgatta taacctaatt gtaataattt ctttgctggt
     4561 gttttttttc ttaaaagctc agacaaagtt attttctttt tttttatagg cttttttatt
     4621 gttatttttt ggggtttttc ttttttttct tctttaaatc ctagtgtttt tatttctgca
     4681 gctgtaaaac ctgctttttt aagatccgtg aatgtatatt tcagcccaga gaatattgtt
     4741 cgatttgctt tagctttctg tagatcttta gcaaatctaa tgatacttat ttttttcttt
     4801 cttagtaatg atatatcaat tggcgagata ataaatttct ttggagaatt aagctgttta
     4861 tttagctttt ctattatctc ttttttattt agactagcgg ttgctttttt ttcttttaaa
     4921 acaggttttt gtggaattgt ttcttttact ttttttggct catttaatga aaaacctaat
     4981 ttttttaatt ccagattatt aaaacctgca ttttttaagt ctttaagagt gtattttaaa
     5041 cctaagaata tagtataatt tgctttaact ttctgtaaat cttttgcata tctattaata
     5101 cttatttttt tctttcttag taatgatata tcaattggtg agataataaa tttctttgga
     5161 gaattaagct gtttatttag cttttctatt atctcttttt tagttaaact aactgttgct
     5221 ttttttattt ttttcatatt tttctctctc ttaaaaatat taactaaatc cggttatttg
     5281 ttatatatgt tatgtatgaa aagctttata atcttaaggc aaaagaaagc tttatatact
     5341 gtttttaaaa tagatatata taaggcttaa atcttaagct aaaccatttc tattacaaaa
     5401 aataaggggg taaaataaat ggcaaaaaaa gaaacaaaaa taggagctta tatatttatt
     5461 gcaggaattg taattgcaat aatttcaggc atattaatga attttataag tccagtagta
     5521 attacatctt tgttaatgat acttggtcta attgtaggat tcttaaatat aacagacaaa
     5581 gaagttaaag actacttact tacagcagtg tgtcttgtaa ttgttacatc tcttgggggg
     5641 gcatcaatac aaaatttaat gattattggt ccttacttag caggagtatt acacgcaatt
     5701 atgatattca caatacctgc tacaatagta gttgcattaa aagaaataat ttctatagct
     5761 aaaaactaaa gaaatttatt cttttttctt tttcttattt tattctaaaa ctagttttac
     5821 tttattctac agttactgat tttgcaagat ttcttggttg atcaatagca cagtctttaa
     5881 agtctgctat ataataagct agaagttgca ttggtataac attaacaaaa ggcgttaaat
     5941 ttgagcaggt tattggaaca aaaataacat gatcgcataa tttctctatc tcttcatctc
     6001 cttgagtagc tattgctatg atacttgccc ctcttgcctt tgcctcatgt atgttagata
     6061 aaactttatc atagtttaaa tcactgttgg caataactac aacaggcaag cccttgtcaa
     6121 ttaaagcaat cggaccgtgc ttcatctctg ctgctggata gccttctgcg tgaatataag
     6181 atatttcttt tagttttagg gcgccttcta aagcgattgg ataatttatt cctcgtccaa
     6241 gatatagtgt gtttttacaa atgtaatatt tttttgcaat ttcttttatt ttttcttcat
     6301 tttctttttt taactctgct cttattatgt ggggtaattc taaaagatta tttattaatt
     6361 ttttagcgcc ggagtcatct atactcccga taattctacc aagatatagg gttaaaagat
     6421 tcaaacaaac tagttggctg gtaaatgctt ttgttgaagc aacacctatt tctggtcctg
     6481 ctctaatata tataccacca tcacattctc tagcaattgt tgaaccaaca acattaacaa
     6541 taccaattac ttttgcacct tttttctttg cttctctaac tgctgcaaga gtgtctgctg
     6601 tttctcctga ttgacttatt gctataatta aagtattatt atcaattatt ggatttctat
     6661 atctaaactc agaagcataa tcaacttcta ctggaattct taaaaaacct tccatagaaa
     6721 actttgcgac aagaccacaa tgccaggagg taccacaagc aacaataatt attcttttta
     6781 tattttttat ttgctctgta gttaggttaa taccacccaa ttttgcagta ttattctcaa
     6841 aatcaattct tcctaagatt gcttgcttta tagtttctgg ttgctcatgt atttctttta
     6901 acataaaatg tttatagcct tgcttttcta tttcttctag atttgtttct atttctatgg
     6961 tttctttatc taaagttata ttgtcaagag atgtaatttc acaaccacca ttaatacaag
     7021 ccatttcatt atcttctaaa tacattactt tctttgtgtg ttcaattatt gcagaaacat
     7081 cgcttgccac aaaatattcg caatctccaa ttccaataac tattggagaa ccgcgcctag
     7141 caacaataag ttctttacct tgcttatcta taactacaat accaaaagta cccacaactt
     7201 tctttaaggc cttttgaact gcttttttta agtctcctga ataaaacttc tcaattaagt
     7261 gtgctaatac ttcggtgtct gtttcagact tgaaaacatg cccttctttt tctagaaatt
     7321 tctttaaagt ttcgaagttt tcaattatac cattatgtac taatgcaatt tctgttttac
     7381 aatcagtatg tgggtgagca ttaattacag taggttctcc gtgtgttgcc ca
//
LOCUS       DAOOON010000032         7250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_12649_length_7250_cov_14.480751, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000032 DAOOON010000000
VERSION     DAOOON010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12649_length_7250_cov_14.480751"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1822
                     /locus_tag="PLK55_03765"
     CDS             362..1822
                     /locus_tag="PLK55_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOZ36071.1"
                     /translation="MLSIMDLEKQNPWWKDKKNINNDPKIQAIEDATIKWMPEIITEF
                     DFTKDIVYSLRGPRQTGKTTLTKIIIKNELKTKNPKAIFYFSCDLIISDAKELEETIG
                     TYLDWAESLTNERKLIIIDEISEVKHWEKAIKKIIDERSNKNKTFILTGSHSIDIYRS
                     VEKLPGRRGEDTGTFTHKVFYPMSFKEFVRITKPKLFTEINKMINNDNNEFINICNNI
                     IPESLNKLQLYKSELDYALDEYLLTGGFISVINNYKNNNKIENTLYEEYIRSIFGDFA
                     KVNKNQKYVFQILSSIIKKETSQVSWTAIMKDTHIKDSRTVENYVEILQGMLIVDAFY
                     PIDKNTKQIKYSLTDKKIYLNNPFLFHAFRALINGSINYFEDAKLYLNKENKANLLEF
                     VFGNHLLRKINKMYPSDLTYINEKVYFFKGKKEIDFVIKLENKLIGFEVKYQNQITKS
                     DYSPLFVLDKGVLISKNTFETEKYMCIPISMMLMYL"
     gene            complement(1984..4386)
                     /locus_tag="PLK55_03770"
     CDS             complement(1984..4386)
                     /locus_tag="PLK55_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ36072.1"
                     /translation="MRLKVYSVLFIFILLFISNFVYSEEIFNKEINSEDNINNKYQQD
                     VFYPPEEALTKQGQTLESYIQSLKDGAPRNRNSSVDNIWGTSPLTGNIHIPVLLVQFT
                     DNMPTLSASDINTAFNSPNYLGGAGISVSKYYTKESYGDLNITFDIYGWQTAPNTYSY
                     YSLNSNRNFEMELATINMFNSTIDFAQYDNDGSGRIDGYIIIIPGPNGIAPTGIWPHA
                     RILRNYNSNMVDGKYLGNVAVVPELGYSDAQFEIPITTHEFAHVLGLMDLYSNTSTGQ
                     GTGPLKDFTMMNFNFSPYCLDKPINLDAWSRYFFGWTNPLILTTDSPKEISLRSANDY
                     SDSVILKNSYMTPREFFIIENRYRENVPSNLDKCMFNNSPLPRSGFAIYHVDENKIEY
                     DYPNNYVNWDRDGNWRDDTVSHPGILYEENFIDDPIDYTANTLDFYFNEQIYGCDTFR
                     FFDENKRICTNLYAIDDSTTRAYSGVENPFIRFEALSSTNQSTMTAKMLVGEETAIPI
                     ANPASGNYDSTIQVTLETPTVGATIYYTLDGSTPTINSQIYSSPTTISQTTTLKAIAK
                     KDNFYVSDVLIENYTINQTTSSPLSSLPSGNYPRWTQIELSTTTPNSNIYYTLDGTTP
                     NVSSTLYQSPITIDHNFTLKAIAYSSGYSPSQVSTYYYTTNDIPEVAEPIANISGGTY
                     SSTQIVELTTTTPNAIIRYTLDGTEPNRIKSLYYNGSITISQNTTLKAKAYLYTGPTV
                     LSSQGGSKSGSTALLGLSNLSQATVSWYSSSTMLEEYVISRQSKHKNTISKSLPNISQ
                     NK"
     gene            complement(4517..>7250)
                     /locus_tag="PLK55_03775"
     CDS             complement(4517..>7250)
                     /locus_tag="PLK55_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36073.1"
                     /translation="MIVKKKKAQTALEILVIFGILIVGAIIFALFYSTNIEKKLDTSS
                     NIQGTYSDIENNLQSNDPQQPIETCGNDFCGPGENCIGCPDDCDCTNCPSTAPILQIT
                     RTPVTGVPAGAPGNNIFQLQVLVSRQLEFSGKTIKIKKVIFNESQTDVFYKGQTQIPI
                     TGLEINQEMVESTPGNFLYNITNLSSTKSGVYVIKVFAEVSGCDLNSEAKTMITIKGL
                     SANLSLSPDGTSNRDDPFGVDINVNNYNSDSNVSITKIYVLDTNNTLLDNVCSYNGNL
                     ISSSGQVVNILMNKTYNDYNYSFKDTFSCKYSGQYKLMFTLHDNDENTDYDYNATIEK
                     SIKDRCLYLGNKGEGTESKPYVISNPGDLDCIRYNLSKHFILGNNIDLNHALMSSNSE
                     YYWYDDTKGWNPIGTLDAAFTGSFDGADYEILNLYSRRNDSSFRFALFRNVDNSSFSN
                     LNLTGVDINGHSLSGSLVFSASNSTFDNINVEGSIGTNGVGGIVGSSANNYFNNCSFT
                     GEIRGVINVGGIVGESFYANETGPNANSFNDCTVDINISGPTYAGDGYFTDSAYIAVG
                     GIIGAVHSYGTTISDCNVTGNIKSQGFTGGLVGEGGGIEINNSSFIGNINSHVNVGGL
                     IGYGSADVIKSHAEVNITNAAFQPAIGNASIPGLWGGIGGHIGGSIVNSSAIVNMQPI
                     SISESNQYVGYMGGLIGWSEAVVIQNSYSEGDIIIADNKIYEGRYIGGLAGHLQNIWP
                     DYEYGIKDNYSKVNISTDMAGYRIGGLFGSASSNYDINNSYSIGNINVSGTKTNVGGL
                     IGIKSSTVAISNSYWNTETSGQTTSAGGSPRTTDQMTYPDYPSTYTDWDFSSVWAEDM
                     EGTNDGYPYLLPSPPSVDLRGDPAPKVVEEGGEVFSYILVSEDLNILDYGIIYN"
BASE COUNT         2522 a         1133 c         1096 g         2499 t
ORIGIN      
        1 catcttgttg atatttattg tttatattat cttctgaatt tatttcttta ttaaatattt
       61 cttcagaata tacaaaatta gatataaata ataatagaaa tattaagaat aagatatttg
      121 ctttctttgt catatatata atatatacaa tatcttttaa ataattatct aaataaataa
      181 ctgtagacag ttattttctc ttattcctgg ttatttctag taactggttt tgggcatttg
      241 tggttgctgg taatgacaac agttaacaac ctttaaacag ggaatgagtt ccagcagcat
      301 ttatataggc taccggaatg cattacttaa gtttatatgc tttttggtac atatatgtgt
      361 tatgctatca ataatggatt tagaaaaaca aaacccttgg tggaaagata aaaaaaatat
      421 aaataacgat cctaaaatcc aagcaataga ggacgccaca attaaatgga tgcctgaaat
      481 aattacagag tttgatttca caaaagacat tgtttacagc ttaagggggc caagacaaac
      541 cggcaaaaca acattaacta aaataattat aaaaaatgaa ctaaaaacaa aaaatccaaa
      601 agctatattc tattttagtt gcgatttaat aataagcgat gccaaagaat tagaagaaac
      661 aattggcaca tatcttgatt gggcagaaag cttaacaaat gaaagaaaac taattattat
      721 tgatgaaatc tcagaagtaa aacattggga aaaagcaatt aaaaaaataa tagatgaaag
      781 atcaaataaa aataaaactt ttatacttac cggatcacac tctatagaca tatatagaag
      841 cgtagaaaag ctaccaggaa gacgaggaga agacactgga acatttacgc acaaggtttt
      901 ttatcccatg tcttttaaag aattcgtaag aataactaaa cccaaactat ttactgagat
      961 aaataagatg attaataatg acaataacga atttataaac atttgcaata atataatccc
     1021 tgagtcatta aataaattgc aattatataa atcagaatta gattatgcgc tagatgaata
     1081 tttattaacc ggggggttta tttcggtaat taataattat aaaaataata ataaaataga
     1141 aaacacgtta tatgaagaat atataagatc aatatttgga gattttgcaa aagtcaataa
     1201 aaatcaaaaa tatgtttttc aaattttgtc ttcaattatt aaaaaagaga cctctcaagt
     1261 ttcttggacc gctataatga aagacaccca cattaaagac tctagaactg ttgaaaacta
     1321 tgtagaaata ttgcaaggca tgctgattgt agatgcgttt tatcctattg ataaaaacac
     1381 aaaacaaata aaatattctt taactgataa aaaaatatac ctgaataatc ccttcttatt
     1441 tcacgcattt agggcattaa taaacgggtc aataaattat tttgaagatg caaaattata
     1501 tttaaataaa gaaaacaaag ctaacctact agaattcgtc tttggaaatc acctactaag
     1561 aaaaataaac aaaatgtatc ctagtgattt aacatatatc aatgagaaag tatatttctt
     1621 taagggtaaa aaagaaattg actttgtaat aaaattagaa aacaaattaa ttggatttga
     1681 ggtaaaatat caaaatcaaa taactaaaag tgattattcg ccattgtttg tattagataa
     1741 aggggtatta atttctaaaa atacttttga aaccgaaaaa tacatgtgta ttcctatttc
     1801 aatgatgtta atgtatttat aacggaaaag ggataattaa aaaacaaata atttttttta
     1861 cttaatattt gtgattattt tatttattaa ttctataata ttcacttact aaatatttag
     1921 taaataaata tataataaag acattatata attctataaa aatatatatt acttaattag
     1981 catttattta ttttgagaaa tatttggcag agatttagaa attgtgtttt tgtgttttga
     2041 ttgccttgag attacatatt cttcaagcat tgttgaacta gaataccaag aaactgttgc
     2101 ttgagaaaga tttgataaac ctaaaagggc tgttgaaccg ctcttggaac cgccttgaga
     2161 actaagaact gttggaccag tataaagata agcttttgct tttaatgttg tattttgaga
     2221 aatggttatt gaaccgttat aataaagact tttgattctg tttggctctg tgccatcaag
     2281 agtgtatcta ataattgcgt ttggagttgt tgtagttaat tctactattt gtgtggaact
     2341 ataagtgcca ccagaaatat ttgcaatagg ttcagcaact tcagggatat cgtttgttgt
     2401 ataataataa gtggaaactt gactagggga ataaccagaa ctataagcaa ttgcttttaa
     2461 ggtgaagtta tggtcgattg ttattggtga ttgataaaga gttgaagata catttggtgt
     2521 tgtgccatca agagtataat aaatatttga atttggtgtt gttgtagata attcgatttg
     2581 tgtccaccgc ggataattgc cagaaggtaa actggaaaga ggggaagaag tggtttgatt
     2641 aatcgtgtag ttctcaatta aaacatcact aacataaaaa ttatctttct tggctattgc
     2701 ttttagagtt gtggtttgtg agatagttgt tggtgaagaa tagatttgag aattaattgt
     2761 tggagtgctg ccatcaagag tataatatat tgttgcgcca actgttggag tttctaaagt
     2821 tacttgtatt gttgaatcat aattgccaga agcaggattt gctattggta tcgcagtttc
     2881 ttctcccact agcatcttag cagtcattgt tgattgatta gttgaagaaa gagcttcaaa
     2941 gcgaatgaaa ggattctcta caccgctata tgctcgagtt gttgaatcat caattgcata
     3001 aagatttgta cagattcttt tattttcatc aaagaatcta aaagtatcac aaccatatat
     3061 ttgttcatta aaataaaaat caagtgtatt tgctgtataa tcaataggat catctataaa
     3121 attttcttca tataatattc ctggatgact tacggtgtca tctctccaat tgccgtctct
     3181 atcccagtta acatagttgt ttggataatc atattctatt ttgttctcat caacatgata
     3241 gattgcaaaa ccgcttcttg gtaatggtga gttattaaac atacacttgt ctagattact
     3301 ggggacgttt tctctgtatc tgttttctat tatgaaaaat tctcttggag tcatataact
     3361 gttttttaga atcactgaat cagaataatc gtttgcactt cttagactta tttctttagg
     3421 actgtctgtt gttagaatca agggattagt ccagccaaaa aaatatcttg accaggcatc
     3481 aagattgatt ggcttatcaa gacaatatgg agaaaaatta aaattcatca ttgtaaaatc
     3541 ttttaacggg ccagttcctt ggcctgttga cgtattacta tataaatcca ttaatccaag
     3601 aacatgtgca aactcgtgag tggttattgg aatctcgaac tgagcatctg aataccctag
     3661 ctcaggcact actgcaacat taccaaggta ttttccatct accatattac tattataatt
     3721 tctaagtatt cttgcatgtg gccaaatacc agttggtgct attccgtttg gaccaggaat
     3781 aataattatg taaccgtcta ttcttccact accgtcatta tcatactgtg caaaatcaat
     3841 tgttgaatta aacatattta ttgttgctag ttccatttca aaatttctat tactatttaa
     3901 actataatag gaataagtgt ttggggctgt ctgccagcca taaatatcaa aagtaatatt
     3961 tagatcgcca taagattctt ttgtgtagta tttgcttaca ctgattcctg cgccacctaa
     4021 ataattagga ctattaaaag cggtgttgat atctgatgca gaaagagtag gcatgttatc
     4081 tgtaaactga actaacaaaa ctggtatatg aatatttcct gttaacggac tagtgcccca
     4141 gatattgtca acacttgagt ttctgtttct tggtgctccg tcttttaaag attgaatata
     4201 gctttcaagt gtttggcctt gctttgttaa agcctcttct ggcggataaa aaacatcttg
     4261 ttgatattta ttgtttatat tatcttctga atttatttct ttattaaata tttcttcaga
     4321 atatacaaaa ttagatataa ataataatat aaatataaat aaaacagaat atacttttaa
     4381 tctcataaat atcttagaac aaacggtttt ttaagtgttt tgaaatcaat taactgaatt
     4441 ggattataaa acacataaaa ttacaatcta aaaaataata attaaaaaaa taaataaata
     4501 aaatataact aatagattag ttataaatta ttccataatc caaaatattc aaatcttcag
     4561 aaactaaaat atatgaaaaa acctctccgc cttcttccac aacctttggt gcggggtctc
     4621 cgcgtaaatc tacgcttgga gggctaggca aaagatatgg ataaccgtca tttgttcctt
     4681 ccatgtcctc agcccaaaca cttgaaaagt cccaatctgt atatgtgctt ggataatctg
     4741 gataagtcat ttgatcggtt gttcttggag atccccctgc gcttgttgtt tgcccagaag
     4801 tttctgtgtt ccagtaacta ttacttatgg caacggtact agatttaata cctattaatc
     4861 caccaacgtt tgtttttgtt ccagaaacat ttatattgcc tatagaataa gaattattaa
     4921 tatcataatt actagatgca gaaccaaata atccccctat tctgtaacct gccatatctg
     4981 tgcttatatt taccttagaa taattatctt taataccata ttcgtaatct ggccagatat
     5041 tttgtagatg acctgctagt cccccaatat atcgcccttc atatattttg ttatccgcta
     5101 taatgatatc tccttctgaa taagaatttt gaataactac tgcctctgac caaccaataa
     5161 gtccccccat ataacctaca tattgattag actcagaaat tgaaattggt tgcatattta
     5221 cgattgcaga actattaact atagaaccgc caatgtgccc gcctatgccg ccccaaagtc
     5281 caggaataga tgcatttcca attgctggct ggaatgccgc atttgttatg tttacctcgg
     5341 cgtgagattt gataacgtct gcagacccat aacctatcaa accaccaaca tttacatgtg
     5401 aattgatgtt gcctataaaa gaactattat ttatctctat tccccctcct tcgccaacta
     5461 atcccccagt aaatccttga gatttaatat tgcctgtaac attacaatcg gatattgtgg
     5521 tgccataaga atgcactgct ccaattatgc cgccaacagc tatgtaagca gaatctgtaa
     5581 agtagccatc gcctgcataa gttggcccag aaatgtttat atcaactgta caatcattaa
     5641 aactgtttgc gttaggaccg gtttcattag cataaaacga ttcaccaaca attcccccga
     5701 cgtttatgac tccacgtatt tcgccagtaa atgaacaatt gttaaaataa ttatttgcag
     5761 aagagccaac tatgcccccc accccgtttg ttcctatgct cccttcaaca tttatattat
     5821 caaacgtaga attggatgca gaaaatacta aagaaccaga taaagagtga ccattaatat
     5881 ctacccctgt taaatttaaa ttagaaaaag aagaattatc aacgttacgg aataatgcaa
     5941 aacggaaact tgaatcgttt cttctggaat ataaatttag tatttcataa tccgcaccat
     6001 caaaagaacc agtaaatgct gcatctagtg tgcctattgg attccaacct tttgtgtcat
     6061 cataccaata atattctgaa tttgaagaca taagtgcgtg atttaaatct atgttatttc
     6121 ctaatataaa gtgcttactt aaattgtatc taatacaatc taaatcccct ggattagaaa
     6181 ttacataagg tttagattct gttccttctc ctttatttcc taaatataaa caacgatctt
     6241 ttatactttt ttcaattgtt gcattatagt cataatctgt gttttcatca ttatcgtgta
     6301 acgtaaacat taacttatat tgccctgaat atttacagct aaaagtatct ttaaaagaat
     6361 aattataatc attataagtt ttattcatta atatatttac aacttgacca gaactactta
     6421 ttaaattccc gttatatgaa caaacattat ctaataaagt attatttgta tctaaaacat
     6481 aaatttttgt aatacttaca ttactatctg aattgtaatt attaacatta atatctactc
     6541 caaatgggtc atcgcgatta cttgtaccgt ctggcgacaa acttaaattt gctgatagtc
     6601 cttttatagt tatcatagtt ttagcttctg agtttagatc acaaccagaa acttctgcaa
     6661 aaacttttat tacatagacg cctgattttg tacttgaaag attagtaata ttatataaaa
     6721 aattgccagg tgtgctttca accatttctt gattaatttc aagacctgtt attggtattt
     6781 gggtttgccc tttataaaaa acatctgttt gactttcatt aaatattact ttttttattt
     6841 taatagtttt accagaaaat tctaactgtc ttgaaactaa tacctgtaat tgaaaaatat
     6901 tatttcctgg agcacctgca ggaacgccag taacaggcgt gcgtgtaatt tgtaaaattg
     6961 gggcagttga tggacaatta gtacaatcac aatcgtctgg gcaacctata caattttctc
     7021 ctggaccaca aaaatcatta ccgcaagttt ctattggctg ttgtgggtcg ttactttgta
     7081 gattgttttc aatatcagaa taggtgcctt gaatatttga agaagtatct aatttctttt
     7141 caatattggt tgaataaaat aatgcaaaaa taattgcccc tacaataaga attccaaaaa
     7201 ttactaatat ttctagagca gtttgtgctt tctttttttt tacaatcatt
//
LOCUS       DAOOON010000033         7068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_13078_length_7068_cov_22.023528, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000033 DAOOON010000000
VERSION     DAOOON010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13078_length_7068_cov_22.023528"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLK55_03780"
     CDS             complement(<1..1319)
                     /locus_tag="PLK55_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOZ36074.1"
                     /translation="MAFLNNIKSFLKRKLFYILVILIILSFTIFVKAISVYLQLFILA
                     VYFYFLILSILISKKYSEISKAEPFPENPPTVAVVTYAFGNWTPIKKTINQLKKLKYP
                     IPFNIYVITDGTCTFLKKDKQVKPILIDKKYFLKSKKNLKSVIINQGLKHITEENIFE
                     VDGDTLPEKDALMKMTGSLKGNVGIVVGVIGVTNTKKFVEKIQAIEYNFSFGLPRMVL
                     TSMGSLDIGTGALCLFKRKDFLAVGGYNEHNITEDKDLAYKLIDRGKTIHFVVDAKAK
                     TDVPNTWKKYYIQRIRWARGGIDTTNRYLSFLFNKKLGIFGYYLPYSLFILIMSMALI
                     FRSAYNIIANFIEYSYYHLLNFIQYGIVWGDITINLSNILNVNSIIIILFVSLLVSIY
                     FMGLSFKYTNHKLDSSYILPIIFLLLIYGFIIIMINIISIIQELFEV"
     gene            complement(1359..1628)
                     /gene="albA"
                     /locus_tag="PLK55_03785"
     CDS             complement(1359..1628)
                     /gene="albA"
                     /locus_tag="PLK55_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HOZ36075.1"
                     /translation="MEGNTIYIGKKGTMSYVLAVITQFNQGTDTVMIKARGKTISKAV
                     DVAEIVKNKFVNTVKVSDIVISTEEITTENNTPMKVSAIEIKLNK"
     gene            1743..2165
                     /locus_tag="PLK55_03790"
     CDS             1743..2165
                     /locus_tag="PLK55_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36076.1"
                     /translation="MPSIEVHCAISKKRTGESFKELHEWIDEPGTLKGAEHRLDRHRY
                     TEEDRDYIKKIWDLKLGAGGGNKAIVEWLFHIAMDYMDVGFKMSNTIYENNQNNVFMF
                     GFNNEGNIHTTFGTIDKTELNKISESYLYDPNADFEYY"
     gene            complement(2162..3364)
                     /locus_tag="PLK55_03795"
     CDS             complement(2162..3364)
                     /locus_tag="PLK55_03795"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOZ36077.1"
                     /translation="MNLEKYRKDFPILNKTTYLDNAATSFKPLSVIKAIDYYYRNCPV
                     NIHRGVYKLSQEASIAYEDCHTDLSKFFNCSPREVILTHNSTDSLNVIMYMLYNSNYF
                     KKGDEILTTVYEHHANLVPWQFLSQKLGLKLKFAEINKDYTLNLSDFKKKITNKTKLI
                     TIAHISNTTAVIAPIKEVVDIAKEKNILVCVDGSQSAPHRKIDFKKLNIDFFVCTMHK
                     MLGPTGVGVLIGKEKLLEKFSPVRFGGDMIEHVTLQRSTWNDLPNKFEAGTPNIAGAF
                     GTLAAIDYLKKVGMDNIERQDKILTDYAFDKLNNIPGLKLYNPCDSKKQGSIVLFDLK
                     GIKSRELSSLLDEYGNIATRAGMHCAEPIVSRFNKDGLVRASFYFYNTKEEIEKLAET
                     LKKIKKII"
     gene            3496..3780
                     /locus_tag="PLK55_03800"
     CDS             3496..3780
                     /locus_tag="PLK55_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HOZ36078.1"
                     /translation="MKIPKQIKTYCRKCKKHQPHKLSQYKSRGARTLAVGTRKNVRKH
                     KKGYGGMAKHIATVKKQNKKPVFTAECSVCKAKHYYVIPKKMKKTEFKTD"
     gene            3839..4513
                     /locus_tag="PLK55_03805"
     CDS             3839..4513
                     /locus_tag="PLK55_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36079.1"
                     /translation="MKFDTIYKFVAASLAIAYFIGLLVGLVVFNFNIRNYQETDITRS
                     FYFSGQISFSEVFSETLESSFKIAILPFSFIIIGTKTGFTHATYILSPFLGQIKLATL
                     LIPAIFYFITYIILSAIGIKLFATIIIFFVNKILKKEKKQIIKTKLLQKDDIFLLYVA
                     IVSIIIGAIIQVYLSRIFFIFLINLQLITYILIIIIYLGIIIVSFTILYNIIKSVIGN
                     NKQKLW"
     gene            4517..>7068
                     /locus_tag="PLK55_03810"
     CDS             4517..>7068
                     /locus_tag="PLK55_03810"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HOZ36080.1"
                     /translation="MGIDFESEEIIVGRIQSHIKKYGKKGTGYIGKVVVSSGEEPVLG
                     KKVLMDVSRSHVAIVCGKRGGGKCLTGNTLITLEDGREIPIKDLEKENKKIISLQNNL
                     KLKCNKKYQFYKRTADKTLKITLRSGKKIELTPEHPLLTINGWKESKELKKGSRIATP
                     RIIPKFGNKKLPDCEVKLLAYLIAEGHLGNSFVLFCNTDKKIISEFTAAVNKFDNKLT
                     INKHGNFNLRIVDNNNIRKIIKNQRNDRQQFSKGIVFDKKSSIRKYLEKINLYNVKSE
                     TKFVPETIFQLPKEQLSIFLNRLFSCDGTIYFDKNKNEYNISYSSSSPKLIQQIQSLL
                     LRFGLLSIIRTKKTKKLPNYELKILSSQNIEFIKKIGFFGNKKEIQKKVKLKSSNPNV
                     DTIPKEIWNIYRPKNWAIIGKEFGYKLPKALRSSINNSPSREKLFKIANFEKNEELKK
                     IATSDIFWDEIQDIKKINKKQIVYDITVPETHNFVANNIIVHNSYTMSVLIEEFAKQP
                     FEIKERLSVIVIDTVGIFWTMKYPNKDETELLEKWNLKPDGIEIRNLVPFGKKDFYEQ
                     KNLPHDGYFSIRTSQMDIEDWLGVFRLNWRDAESSLLSRTIEKIKNKVGSLFDIDDIM
                     NETKKDAETSQEIINSLNNRFLVAKSWGLFSRSGTTMKEFAKPGTITIVDTSTYKEAI
                     GMESIKELIVGILGKRLYEERMLYRKEEEMSLLTGGRKESEMPIIWMMIDEAHMFMPS
                     DRQSMALDVLLQWIRVGRQPGLSLLLATQRPNKLHNEAISQCDFFLSMRMTSQEDIMA
                     VSSIRPSYLNIPMDKYYGQMPKDQGFAILLDDNSEKVMLIKVRPRITWDGGKT"
BASE COUNT         2640 a         1013 c         1048 g         2367 t
ORIGIN      
        1 tgcacctcaa ataattcttg tataatacta ataatattta tcattattat tataaaacca
       61 tatatcaaca acagaaaaat aattggcaga atataacttg aatctaattt gtgatttgta
      121 tacttaaaag acaggcccat aaagtatatt gaaaccaata aagagacaaa taagattatg
      181 ataattgaat taacatttaa aatgtttgat aaattaatag taatgtcccc ccaaacaata
      241 ccatattgaa taaaatttaa tagatgataa taagaatatt caataaaatt tgcaataata
      301 ttataagcag atctaaaaat taaagccata ctcataatta atataaataa agaatagggc
      361 agataataac caaagatacc caacttttta ttaaataaaa aagacaaata tctatttgtg
      421 gtatcgattc cccctcttgc ccatcttatt ctttgtatat aatatttctt ccaagtattc
      481 gggacatcgg tcttcgcttt tgcatcaacc acaaaatgga tagttttccc tctatcgatt
      541 aatttatatg ctagatcttt gtcttctgta atattgtgct cgttgtaacc accaactgct
      601 agaaaatcct ttcttttaaa taagcataaa gcgccggttc caatatctaa tgaccccatt
      661 gatgttaata ccattctggg taacccaaaa ctaaaattat attctatggc ctgaatcttt
      721 tcaacaaatt tttttgtatt tgttactccg attactccca caacaatacc aacattccct
      781 tttaaagaac cagtcatctt cattaatgcg tctttttctg ggagggtgtc tccatcaact
      841 tcaaaaatgt tttcttcggt tatgtgcttt aggccttggt ttatgattac tgactttaga
      901 ttcttcttac tttttaaaaa atatttcttg tcaattagta taggctttac ttgtttatct
      961 ttttttaaga atgtacatgt gccatctgta ataacataaa tattaaatgg aataggatat
     1021 tttaattttt ttaattggtt tattgttttc tttatcggtg tccaattacc aaaagcatat
     1081 gttacaactg caacagttgg cgggttttct ggaaatggct ctgcttttga aatttcagaa
     1141 tattttttag aaataagtat agacaatatc aaaaaataaa aataaacagc caaaataaac
     1201 aattgaagat aaacgcttat ggcttttaca aagatagtga aagacaaaat aattagaatg
     1261 accaatatat aaaataattt tcgttttaaa aatgatttta tattatttaa gaatgccata
     1321 tttttcagaa tatcaaaaat ttaaaaaaag aaataaaatt atttatttaa ttttatttcg
     1381 attgctgaca ctttcatagg ggtattattt tctgtagtta tctcttctgt tgaaataact
     1441 atgtcagata cctttactgt attaacaaat ttgttcttca caatctctgc aacatcaacg
     1501 gctttagaaa ttgtctttcc tcttgctttg atcataacag tatctgtgcc ttgattgaat
     1561 tgtgttataa ctgctaatac atatgacatt gtaccttttt ttccgatgta tattgtattt
     1621 ccttccattt cgctcattgt atatcatccc cctttaatag atgtattatt gaattatata
     1681 tttatttaaa caaaatgttt aaaaacaggc atttttatat atttatattg gaggtaatta
     1741 ctatgcctag tattgaagta cattgtgcaa tttcaaaaaa aagaacaggg gaaagtttta
     1801 aagaactaca tgaatggatt gatgaacccg gaactttaaa aggagcagaa catagacttg
     1861 atagacacag atatacagaa gaagatagag attatattaa gaaaatatgg gatcttaaac
     1921 ttggtgcggg tggcggaaac aaagccatag ttgaatggtt atttcacatt gctatggact
     1981 atatggatgt tggatttaaa atgtcaaata ctatttatga aaataatcaa aacaatgtat
     2041 ttatgtttgg atttaataat gaaggaaaca tacatacaac atttggtacg atagataaga
     2101 cagaattaaa caaaatttca gaaagctatc tttatgaccc aaatgcagac tttgaatatt
     2161 attaaattat tttttttatt tttttcaaag tttctgctaa tttttctatt tcttcttttg
     2221 tgttatagaa ataaaatgag gccctaacaa gcccatcttt attaaatctc gaaacaattg
     2281 gctctgcaca atgcatacct gcacgcgttg caatgttacc atattcgtca agaagagaag
     2341 aaagctctct tgattttatg ccttttaaat cgaaaaggac aattgaacct tgctttttag
     2401 aatcgcaagg attgtaaagt tttaaacctg gaatattatt taatttgtca aacgcataat
     2461 ctgttaaaat cttatcttgt ctttcaatat tatccattcc cacttttttt aaataatcaa
     2521 ttgcagctag tgtcccaaag gcaccagcaa tgtttggcgt tcctgcttcg aatttatttg
     2581 gcaaatcatt ccaggttgat ctttgtaaag taacgtgctc aatcatatcc ccaccaaatc
     2641 ttaccggaga aaatttttca agaagttttt ctttaccaat caacacgcca acacctgtag
     2701 gcccaagcat cttatgcatt gtacaaacaa agaaatcaat atttaatttt ttaaaatcga
     2761 tttttctgtg tggtgcagac tgagaaccat caacacacac taaaatgttt ttttcttttg
     2821 caatatctac aacctcttta ataggagcaa taactgctgt ggtgtttgag atgtgcgcta
     2881 tagttattag ctttgtttta tttgttattt tctttttaaa atcagaaaga tttaaggtat
     2941 agtctttatt gatctctgca aattttaatt tcaaaccaag cttttgagaa agaaactgcc
     3001 aaggcacaag gtttgcgtgg tgctcataga ctgttgttaa aatctcgtct cctttcttga
     3061 aataattgct attgtatagc atatacatta tgacattaag agaatcagta ctgttatgag
     3121 tcaatataac ttctctagga gaacaattaa aaaatttaga aagatctgtg tgacaatcct
     3181 cataagcaat gcttgcttct tgagataatt tgtaaactcc tctatgaata ttaactgggc
     3241 aatttctata ataataatct atggctttaa ttacagaaag aggtttaaaa cttgttgcag
     3301 cattatctaa atatgttgtt ttgtttaata ttggaaagtc ttttcgatat ttttctaaat
     3361 tcatgtgttt ttcacgttta cctcttgaaa ttaatatata ggcaatcttt ttaaataaat
     3421 atgggacaat atatattata tagagtaagc taaaaaagga ctcttttttt aatatttcta
     3481 aaaaaatgat tatatatgaa gataccaaag caaataaaga catactgtag aaaatgtaaa
     3541 aaacaccagc cacataagct ttcacaatac aaatctagag gcgcaagaac cttggccgta
     3601 ggtactagaa aaaatgttag aaagcacaaa aaaggttatg gtggaatggc aaagcacatt
     3661 gcaacagtta aaaagcagaa taaaaaacca gtgtttactg cggaatgctc tgtatgtaaa
     3721 gcaaagcatt actatgttat tccaaagaaa atgaaaaaaa cagaatttaa gaccgactaa
     3781 agtctttatt tttctgtttc tttagtattt ttaaattcat ctataatata tttatagtat
     3841 gaaatttgat acaatatata aatttgtagc cgctagtttg gctatagctt attttattgg
     3901 attgcttgtg ggacttgttg tttttaattt taatattaga aattatcaag aaacagatat
     3961 cactagatct ttttatttct ctggacaaat tagttttagc gaagtctttt cagagacgct
     4021 agaaagttct tttaaaatcg ctatattgcc tttttctttt attattattg gaactaaaac
     4081 aggatttacg catgcaacat atatattgtc accattttta gggcaaatta aacttgcaac
     4141 tcttttaatc cctgctattt tttattttat aacttatatt attttgtctg ctattggaat
     4201 aaaattgttt gctacaataa ttatattttt tgtaaacaaa atacttaaaa aagaaaaaaa
     4261 acaaattata aaaacaaaat tattacagaa agacgatatc tttttattat atgttgcgat
     4321 tgtctcgata attattgggg caataataca agtatatctt tctagaatat tttttatatt
     4381 tttgattaat cttcaactta taacttatat tctaattata attatatatc ttggaattat
     4441 tatagtttct tttactatac tttataatat aattaaatca gttattggaa ataataaaca
     4501 aaaattatgg taaaatatgg gaattgattt tgaatctgaa gaaataattg tgggcagaat
     4561 ccaaagccac ataaaaaaat acggaaagaa aggaacaggc tatataggta aagtcgtggt
     4621 atctagcgga gaagaaccag tacttggaaa aaaagtttta atggatgtct ctagatcaca
     4681 tgttgcaatt gtttgcggaa aaagaggtgg gggtaaatgc cttactggaa acaccttaat
     4741 tacattagaa gacggcagag agataccaat caaagattta gaaaaagaaa ataaaaaaat
     4801 aatttcttta caaaacaatc ttaaattaaa atgcaataaa aaatatcaat tctataaacg
     4861 tactgctgat aaaactttaa aaataacctt aagaagcggt aaaaaaatag aattaacccc
     4921 cgaacacccg ctattaacaa taaatggttg gaaagaatct aaggaattaa aaaaaggaag
     4981 tcgtattgcc acccccagaa ttattcctaa atttggaaat aaaaagcttc ctgactgcga
     5041 agttaaatta ttagcgtatt taatcgcaga gggccattta gggaatagtt ttgtgttatt
     5101 ttgtaatact gataaaaaga ttatttcaga atttactgct gccgtaaaca aatttgataa
     5161 caaactcaca attaacaaac acggcaattt taacctacga attgttgata acaataacat
     5221 cagaaaaata atcaaaaatc aaagaaatga cagacaacaa tttagtaaag gcatcgtttt
     5281 tgataaaaaa tcttcgatta gaaaatatct tgaaaaaatt aatttgtata atgtaaaatc
     5341 agaaactaaa tttgtcccag aaactatatt tcaattacct aaagaacaac tttccatttt
     5401 cttaaataga ctgttttctt gtgatggcac aatttatttt gataaaaata aaaatgaata
     5461 taatatatct tattcgtctt catctcctaa attaatacaa cagatccaaa gtttactttt
     5521 aagatttgga ttgctttcga taattagaac aaagaaaacc aaaaaattac caaattatga
     5581 actaaagatc ctttcgtctc aaaatattga atttattaaa aaaatcgggt tttttggaaa
     5641 taaaaaagaa atacaaaaaa aagtaaaact aaaatctagt aatccgaatg tagatacaat
     5701 tcctaaagaa atttggaata tctatagacc aaaaaactgg gcaataattg gtaaagaatt
     5761 tggatacaaa ttaccaaaag cattaaggtc atcaatcaat aattctccat caagagaaaa
     5821 actatttaaa attgcaaatt tcgaaaaaaa tgaagagctt aaaaaaattg cgacaagcga
     5881 tatcttctgg gacgaaattc aggatataaa aaaaataaac aaaaaacaaa ttgtgtatga
     5941 cataacagtt ccagaaactc acaattttgt agcaaataat attattgttc ataatagtta
     6001 cactatgtct gttctgatag aagaatttgc aaaacaacca tttgaaatta aagaaaggtt
     6061 atctgttatt gttattgaca ctgtcggaat attttggact atgaaatatc caaataaaga
     6121 cgagacggaa cttcttgaaa aatggaatct aaaaccagac ggaatagaaa tcagaaacct
     6181 tgtgcctttt ggtaagaaag acttctatga acaaaagaat ctcccacatg acgggtattt
     6241 ctctataaga acttcgcaaa tggatattga agactggttg ggtgtattta gacttaattg
     6301 gagggatgca gaatcgtctt tgctttcaag aaccatagag aaaataaaaa ataaggttgg
     6361 aagcctcttt gatattgacg atatcatgaa tgaaacaaaa aaagacgcag aaacctcaca
     6421 agaaataatt aattctctta acaacagatt tcttgttgct aaatcttggg ggcttttttc
     6481 tagatccgga acaacaatga aagagtttgc aaagccagga acaataacta ttgttgatac
     6541 tagtacctac aaagaagcaa ttggcatgga atccattaaa gaactaattg ttggaatact
     6601 aggtaaaaga ttatatgaag aaagaatgct atacagaaaa gaagaagaga tgagtttatt
     6661 aactggcgga agaaaagaaa gcgagatgcc aattatctgg atgatgattg atgaagcaca
     6721 catgtttatg cctagcgaca gacaatcaat ggcgcttgat gttttgttac agtggattag
     6781 agttgggcgg cagcctggtt tatctttact tcttgcaaca caaagaccaa acaaacttca
     6841 taacgaagca atatcgcaat gtgatttttt cttaagcatg agaatgactt ctcaagaaga
     6901 cattatggct gtttcgtcta ttaggccaag ctatcttaat attcctatgg ataaatatta
     6961 tggacagatg ccaaaagacc aaggctttgc aatacttcta gatgataatt ctgaaaaagt
     7021 aatgctaata aaagttagac ccagaattac ttgggatggt ggaaagac
//
LOCUS       DAOOON010000034         6745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_13834_length_6745_cov_26.116442, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000034 DAOOON010000000
VERSION     DAOOON010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13834_length_6745_cov_26.116442"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..685)
                     /locus_tag="PLK55_03815"
     CDS             complement(95..685)
                     /locus_tag="PLK55_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36081.1"
                     /translation="MKEKYFDVVSSEFFNYPSAEEILKKLNIEKIYVVKEINKREDLD
                     FVFPKAKNTKPAFLFKDLNLYSNLKNKITKNALILGQGSSLSANTNIISSKSEIHLLN
                     PISSETAFDEGLARIAKQNNKIIFFDIREIRYNQNKAIKQTLFILELLKKRNVEFRFV
                     SMAKVPEDLVDPIVLKNFLRNLGIERAMIKRILEDL"
     gene            complement(694..2394)
                     /gene="argS"
                     /locus_tag="PLK55_03820"
     CDS             complement(694..2394)
                     /gene="argS"
                     /locus_tag="PLK55_03820"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOZ36082.1"
                     /translation="MFNFKERFAEEISKITKKDISETLKIIEEPPDFNLGDLSFPCFS
                     LSKELRKSPNDISKDLVEKLKKEKQDIFEYFAVSGYVNIKIKPEILYTETISQVFDNK
                     ENYGKSKTGAKEQYVIDTFNANTFKTLHVGHLRMIVGGDAISRLLSFSGYDSKPVYYG
                     GDVGTHVAKWYWYYLQLPKDKQKIPKENVSKWFGEIYIAAGEKTKDNENSTKEIDNIQ
                     KQILTDKELQKEIARLRDICYDAYMQVKTELGVELVDKIFESETEKKFLEIKDAFFKE
                     HKDIIKESDGAIIADLKDKKLDVLVLIKKNGAPLYGAKDIALVQIKKQKFPKCKHFIY
                     IVGSEQDFYFRQLFGLFSVIYPETTHEHISLGMVNLISGKMASRAGGMVLYEDLRDQL
                     RDKVKQVLTENNLEQDKEIIDKIVFGTIKFEMLKIGFNKTFVFDINSALDLQGDSAVY
                     IQYSGVRAKSILRKANFKGINNTPKPEKLEDDEKRLLNLIMQFPSKVEYASKERKPNI
                     IASHCLDLARAFNKFYGNCPVISDNKALQEQRLALISTYLQTLKNATWLLGIEIPEKM
                     "
     gene            complement(2413..2485)
                     /locus_tag="PLK55_03825"
     tRNA            complement(2413..2485)
                     /locus_tag="PLK55_03825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2450..2452),aa:Arg,seq:tct)
     gene            complement(2541..3617)
                     /locus_tag="PLK55_03830"
     CDS             complement(2541..3617)
                     /locus_tag="PLK55_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOZ36083.1"
                     /translation="MKKQKIKKTEKKVKVEKKPKTEKKEIIHKEKKKSNTFLIIGILI
                     IIILIIGAVIATIVLTKNKKAIITDQDVDHNITITPVDTNVSLTEEEFIEKYSTFMLE
                     TTEFGQKIQTELLENIQVKQKPIFDQITSKLNLNKTEIDSCVAENLNLTAEIGENDAK
                     ILQKIFTDTQFAQMIGLTGTPAIIINNEYAGGYLTYTELKEKIDAAMDGNVVEDQFYQ
                     EGDFFFGSENAKVVVYVYSDYYCGYCKKLAQESLMQLKSEYIDTGKIKYLPKDMISSE
                     PSAAVYANCAGEQGKYFEADLELFNNSSEFLENLQTLQNSILEKYSEDINRINSEYAI
                     LQKWIEENPESFSKIQESLAAQAQ"
     gene            3707..4705
                     /gene="twy1"
                     /locus_tag="PLK55_03835"
     CDS             3707..4705
                     /gene="twy1"
                     /locus_tag="PLK55_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HOZ36084.1"
                     /translation="MTKELISTEKIENLKHKHYGMFGKHSAVEICEWNKKALRGEGVC
                     YKEKFYNVECHRCAQITPAVLWCHQNCIFCWRPAEYMTIDKFTTKDYDNPKEIIDGLF
                     KERKKLLSGFGGFPKVSKAKLKEAEVPSHVAISLSGEPTLYKDLWKLIKELKSRKEIK
                     TIFVVTNGMEPENLLALQKHKNLPTQLYLSFVAPNKELHLKINRPKIKDSWERLQKTI
                     WIMPKLKCRKVIRFTLIKGMNDDKKYIKDFVKIFEKSKADFVEIKSYMYLGYSRKTMA
                     IDNMPKYEECLKYAKAFEKESKDYKISDTCKPSKIILLKHKKSKVDNFIIKKKGRK"
     gene            4706..5584
                     /locus_tag="PLK55_03840"
     CDS             4706..5584
                     /locus_tag="PLK55_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOZ36085.1"
                     /translation="MVELNSELKAVVDALNNEKTICLLAPSFPVDFKFPDIILDLRRM
                     GFTKVVELTYAAKLINYKYINIINENPEKQFICGNCPTIVKLIENQYPDLKDNILDVT
                     SPMVVMARFVKREFGNDYKTIFVGPCFAKKTEAKENSDCVDYALTFKELIEIYDYCEE
                     KNILKNIEENKANREFDKFYNDVTKIYPLGGGVASSMITKDILNLEQVIVCDGPKCIE
                     KAMFDFTNNTKYRFVDILFCEGGCLGGPGIVCKDDLETKKQRLFDYKEKSRYYEPNEN
                     YGKFVHAFGLDIKRKK"
     gene            complement(5734..6726)
                     /gene="radA"
                     /locus_tag="PLK55_03845"
     CDS             complement(5734..6726)
                     /gene="radA"
                     /locus_tag="PLK55_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HOZ36086.1"
                     /translation="MSLEFENQENLEETEREPGKEMSITDLPGIGPASAEKLLAAGYD
                     SLEAIAVASPSELVAVAGIGEGTAIKAIKAARDSLEMGFETAEKILDRRKLIARLTTG
                     SKELDELLGGGIESQSITEVYGMFASGKSQWCFQLCVLAQLPNEKGGLNGGVLYIDTE
                     NSFRPERVAQIAKFRGLDPEEVMKNIFVARAFNSNHQMLLADKAQELLKEKNIKLVIV
                     DSLTSQFRSEFVGRGQLSDRQQKLNGHMRTLQKIAELYNVVVFVTNQVMSRPDVLFGD
                     PTDAIGGNIVKHASKTRIYLRKSSGSKRIAKLVDSPSLPDGEAVYQVTENGITD"
BASE COUNT         2127 a         1123 c         1010 g         2485 t
ORIGIN      
        1 gaatcaaggc cttgaggata agtaagtaac tctatagcta gatatctttt tttatgcctt
       61 aaatttggcc tgattactgc tagttttttc tttttcataa atcttctaaa attcttttta
      121 tcatcgctct ttctatccca agatttctta aaaaattctt aagaactatt ggatcgacaa
      181 ggtcttcagg aacttttgcc atagatacaa accgaaactc aacatttctt ttttttaata
      241 gctctaaaat aaataatgtt tgctttattg ctttgttttg attatatctt atttctctta
      301 tatcaaaaaa aataatttta ttgttttgtt ttgctattct tgctaatccc tcgtcaaaag
      361 cggtttctga ggaaatgggg tttaagagat gaatttctga tttgctcgat ataatattgg
      421 tgttagctga gagactgctg ccttgtccaa gaattaatgc gttttttgta atcttgtttt
      481 ttagattgct ataaagattt aagtctttaa acagaaatgc aggctttgtg ttttttgctt
      541 ttgggaaaac aaagtcaagg tcttctcttt tgtttatctc tttaactacg taaatctttt
      601 cgatatttag tttctttaaa atttcttctg cagagggata attgaaaaac tcacttgaaa
      661 ctacatcaaa gtatttttct ttcattttct ttatcacatt ttctctggaa tctctatgcc
      721 taataaccaa gttgcatttt ttagggtttg aagataagta cttattaatg ctagtctttg
      781 ttcttgaagc gccttgttat ctgatattac tgggcaattt ccataaaact tattaaaagc
      841 acgagctaaa tctaaacagt gacttgcaat aatatttggt tttctttctt tagaagcata
      901 ttctactttg cttggaaact gcataatcaa atttaataat ctcttttcat cgtcttctag
      961 tttttctggc tttggtgtgt tgtttatgcc tttgaaattt gcttttctta aaatgctttt
     1021 tgctcgtaca ccactatact gaatatatac tgccgagtcg ccttgaagat caagagcgga
     1081 attaatatca aacacaaagg tcttgttaaa accaatcttt agcatctcaa atttaattgt
     1141 tccaaacact atcttgtcta ttatttcttt gtcctgctct aggttgtttt cagttaatac
     1201 ttgttttact ttatctcgca attgatctct taagtcctca taaagtacca tgcctcctgc
     1261 acgagaagcc atctttccgc ttattagatt taccatgcca agagaaatgt gctcgtgtgt
     1321 agtttctgga taaatcacag aaaataaccc aaataattgc ctaaaataaa aatcctgttc
     1381 tgaacccaca atataaatga aatgcttgca ttttgggaac ttctgttttt tgatttgcac
     1441 aagcgctata tcttttgcac cataaagtgg tgcgccgttc ttttttatta gcactaagac
     1501 atccagtttt ttgtctttta ggtctgctat gatcgcaccg tcactttcct taattatatc
     1561 tttgtgttct ttaaaaaatg catctttaat ttctaaaaac ttcttttcgg tctcgctttc
     1621 aaagatcttg tctacaagct ccactccaag ttctgtttta acctgcatat aagcatcata
     1681 acaaatgtct cttagtcttg ctatttcttt ttgtaattct ttatctgtta aaatctgttt
     1741 ttgaatatta tcaatttctt ttgtggagtt ttcattatct tttgtttttt ctccagcagc
     1801 aatatagatt tctccaaacc atttagagac gttctcttta ggaatctttt gcttgtcttt
     1861 tggtagttgt aaataatacc aataccattt agcgacatgc gttcctacat ctccaccata
     1921 atatactggc ttagaatcgt aaccagagaa agataaaaga cgagagatag catctcctcc
     1981 aactatcatt cttaagtggc caacatgcaa agtcttgaaa gtatttgcat taaaggtgtc
     2041 aattacatat tgttcttttg cgccagtttt tgactttcca taattttctt tattatcaaa
     2101 aacttgagaa attgtttctg tgtaaagtat ttctggtttt atcttaatat taacatatcc
     2161 actaacagca aaatactcaa aaatatcctg tttttctttt tttagtttct caactaagtc
     2221 tttagaaatg tcatttggag attttcttag ttcctttgat aatgagaaac acgggaaaga
     2281 taaatctcca agattaaaat ctggtggttc ttctatgatt ttaagagttt cagaaatatc
     2341 tttttttgta attttagaaa tctcttctgc aaatctttct ttaaagttaa acataattta
     2401 ttacctttaa agcgagcccg gagggattcg aaccctcgac ccacagctta gaaggctgtt
     2461 gccctatcca ctaggccacg ggcccacaat atatataatt tagaaaagaa aataaataaa
     2521 aatagagcag aaagaagcac ttattgtgct tgtgctgcta aagattcttg aatcttagaa
     2581 aaactctctg ggttctcttc tatccatttt tgtaaaattg catattctga gtttattcta
     2641 tttatgtctt ctgaatactt ttctaaaata ctgttttgaa gagtttgtaa attttctaaa
     2701 aactcggaag aattattaaa caattctaaa tcggcttcaa aatattttcc ttgctctcct
     2761 gcacaattag catatactgc ggctgatggc tctgaactaa tcatatcttt tggtaaatat
     2821 ttaattttgc ccgtatcaat atattcagac tttaattgca ttaatgattc ttgtgctagt
     2881 ttcttacaat aaccacaata ataatcagaa taaacataaa caacaacttt tgcattttcg
     2941 gaaccaaaaa agaaatctcc ttcttgataa aactggtctt ccacaacatt cccgtccatt
     3001 gctgcatcaa ttttttcttt taattctgta taagttagat aaccgccagc atattcgtta
     3061 ttaattataa ttgcaggagt tccagttaaa ccaatcattt gtgcaaattg tgtgtctgta
     3121 aaaatctttt gtaaaatttt tgcgtcgttt tctcctattt ctgcagtaag gttaaggttt
     3181 tctgcaacac atgaatctat ttcagtttta tttaaattta atttggaggt tatttgatca
     3241 aatattggct tttgttttac ttgtatattt tctaaaagtt cagtttgtat cttctgacca
     3301 aattctgttg tttcaagcat aaatgttgaa tatttttcta taaattcttc ttccgttaaa
     3361 cttacatttg tatcgacagg agtaattgtt atattgtgat ctacatcctg atctgtaata
     3421 attgcctttt tgttttttgt taaaacaatt gttgcaatta ctgctccgat tattaaaatt
     3481 attataatta aaattcctat aatcaaaaaa gtgtttgatt tctttttttc tttgtgaatt
     3541 atttctttct tttcggtttt aggttttttt tcaactttta ctttcttttc tgttttcttt
     3601 attttctgtt ttttcaaaat atcacccttt tttaatatgc ttaactataa aaatagtagt
     3661 ataatatatt atatagaata ataatagttt aaaaataagt gtttttatga caaaagaatt
     3721 aatatcaaca gaaaagatag aaaacttaaa acataagcat tatggtatgt tcggcaaaca
     3781 ctctgctgtt gagatttgcg aatggaataa aaaagctctg cgtggagaag gcgtttgcta
     3841 taaagaaaaa ttctataatg tcgagtgcca caggtgtgcg caaataacgc cagcagtcct
     3901 ttggtgccat caaaattgta ttttctgttg gcgacccgca gagtatatga ctattgataa
     3961 atttactact aaagactatg acaacccaaa agaaataatt gacggattat ttaaagaaag
     4021 aaaaaaactg ctttctggtt ttggtggttt tccaaaagtt tctaaagcaa aactaaaaga
     4081 agcagaagtg cctagccacg tggccatttc tctttctggg gaacccacac tttacaagga
     4141 cctttggaaa ttgattaagg aattaaaatc tagaaaagaa attaaaacca tttttgtagt
     4201 aactaacgga atggaaccgg aaaatctttt ggctttacaa aagcacaaaa acctgccaac
     4261 acaactttat ctttcttttg ttgcgccaaa caaagaatta catcttaaaa ttaatcgccc
     4321 aaagataaaa gactcttggg agagattaca aaagacaata tggataatgc caaaactaaa
     4381 atgcagaaag gttattagat ttacattaat taaaggaatg aacgatgata aaaaatatat
     4441 taaagacttt gtaaaaatct ttgaaaaatc aaaagctgat tttgtagaaa taaaatctta
     4501 tatgtatctt ggatattcta gaaagactat ggctatagac aacatgccaa aatatgaaga
     4561 atgcttgaaa tacgcaaaag cttttgaaaa agaatcaaag gactataaga tctctgatac
     4621 ttgtaaacca tcaaagataa ttcttttaaa acataagaaa tcaaaagttg ataattttat
     4681 aattaagaaa aaaggtagaa aataaatggt tgaactaaat tcagaattaa aagcagtagt
     4741 tgatgcttta aataatgaaa agacaatttg tcttttggca ccaagctttc ctgtagattt
     4801 taagttcccg gatattattc tagatcttag aagaatgggt tttactaaag ttgtggaatt
     4861 gacttatgct gctaaactta ttaattataa atatataaac ataataaatg aaaaccccga
     4921 aaaacaattt atttgcggta attgcccaac aattgttaaa ttaatagaaa atcaatatcc
     4981 tgatctaaaa gacaatattc tagacgttac ttcaccaatg gtggttatgg cgcgttttgt
     5041 taaaagagag tttgggaatg attataaaac catttttgtg ggtccttgct ttgctaaaaa
     5101 aacagaagcc aaagaaaatt ctgattgcgt agactatgct ctgactttta aagagctaat
     5161 agaaatctat gattattgcg aagaaaaaaa tattttaaaa aatattgaag aaaataaagc
     5221 aaacagggaa tttgataaat tttataatga tgtgacaaaa atttatccac ttggcggcgg
     5281 cgttgcaagt tctatgataa caaaagacat acttaatctt gaacaagtaa ttgtatgcga
     5341 tggcccaaaa tgtatagaaa aggcaatgtt tgattttact aataatacaa aatatagatt
     5401 tgtagatata cttttctgcg aaggcggttg ccttggagga ccaggcattg tgtgtaagga
     5461 tgatcttgaa acaaaaaaac aaagactatt tgattacaaa gaaaaatcaa gatactacga
     5521 gcctaatgaa aactacggaa agttcgtgca tgcttttgga ctggatataa aacgaaagaa
     5581 atgaattagt atttatccca acgactatct gatttgttat tccaagattt taaaaaatta
     5641 tctaataatt tataatactc tttttcttct ttatttttaa atttaaattt ctttttatta
     5701 atcataatct tcacggatta ttgaatcaca gatttaatct gtgattccat tttctgtaac
     5761 ttggtatact gcttcgccgt ctggaaggct tggggaatca acaagttttg caattctttt
     5821 tgaacctgaa ctttttctta aatagattcg cgtctttgat gcgtgcttaa ctatgttacc
     5881 gccaatagca tctgtggggt caccaaacaa aacatctggt cttgacatta cttggtttgt
     5941 tacaaagact acaacattat aaagctctgc aatcttttgt aaagttctca tgtgcccatt
     6001 aagtttctgt tgtctgtctg ataactgacc tcttcctaca aattctgatc taaactggct
     6061 tgttaaagaa tcgacaatta caagcttaat gtttttctct tttaatagct cttgggcctt
     6121 gtctgctaat aacatttggt gattgctatt aaaagctcta gcaacaaata tgtttttcat
     6181 aacctcttca gggtctagcc ctctaaactt tgcaatctgt gccactcttt ctggtctaaa
     6241 tgaattttct gtgtcaatat aaagaactcc gccgtttaaa ccccctttct cattaggcaa
     6301 ttgtgctaaa acacaaagtt ggaaacacca ttgggactta ccagaagcaa acattccata
     6361 gacttctgta attgactggc tttcaattcc tccgccaagt aattcgtcta gttcttttga
     6421 acctgttgtt aatcttgcta ttagctttct tctatcaagt atcttctctg cagtctcaaa
     6481 gcccatctct agtgaatctc ttgctgcttt aattgcttta attgcggttc cttcgccaat
     6541 acctgcaact gcaactaatt cacttgggga agcaacagct attgcttcta gagaatcgta
     6601 gcctgcagct aaaagctttt cagccgaagc tgggccaatg cctggaaggt ctgttattga
     6661 catctcttta cctggttctc tctctgtttc ttctaaattt tcttgatttt caaattctaa
     6721 actcatggtt tcttccttct tttct
//
LOCUS       DAOOON010000035         6681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_14010_length_6681_cov_14.482191, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000035 DAOOON010000000
VERSION     DAOOON010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14010_length_6681_cov_14.482191"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLK55_03850"
     CDS             <1..721
                     /locus_tag="PLK55_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36087.1"
                     /translation="FYYLYMPKGSSSIGRKQPKRIGDSVFIGTPHGKELGYSTHAKQM
                     IRENPGLLKAVYELSKSRKPFLEDPKSRFQILRQKPKAGQNTDASYILKLKEKKYYIK
                     EVNDTSIDRDYSFDIKHKHGRDGVSEHLAIELLKEQGINVIPAHFSYVDPISKKSFIV
                     YEFTQLRTLDELYKTGEITSIRYKNIKKQVKEIEEEINRKFHSFGLDKYKQIWDIRTD
                     NVFYSTGKNKFYVFDPILLKK"
     gene            736..1191
                     /locus_tag="PLK55_03855"
     CDS             736..1191
                     /locus_tag="PLK55_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOZ36088.1"
                     /translation="MAKGCMFCKIAKGKVPSYKIYENKKVIAFLDINPIAKGHTLVIP
                     KKHFENVFDCKDKILAEMIKATQKIALHYKEKLGCTGVNILNASGASAEQQVPHLHFH
                     IIPRIEGDGHKIWPVTEHVTENLERLAEEIKMEDELEPKKKKKTEENNG"
     gene            1268..2593
                     /gene="amrB"
                     /locus_tag="PLK55_03860"
     CDS             1268..2593
                     /gene="amrB"
                     /locus_tag="PLK55_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOZ36089.1"
                     /translation="MNLKIRKPAVSGTFYPSSQLALTSQLEGFFKDTEIEYSGEIINA
                     LIAPHAGYIYSGKTAMFGYKQLYMDLILRKEDSFKVIILAPNHTEYTNKIIIPDYNSF
                     RTPLGDIKLNSTKIKAEINNSPHEQEHAIEVQLPFLQYIFKKANKEFTIIPIIVGDVN
                     AEEIKALAKDINSEITENTIIIVSSDLSHYQPQEKAQEIDNKTIHNILDRKTTASLDA
                     CGSNPIKVLEEISILRSWKEPKLLNYSTSGDVTRDKKAVVGYASISFTEEKEHLLLRL
                     AHRTIQNAFDARKENFFDLEQSVPKEYLERKGSFVTLTINKRLRGCIGNIKGTKTVFQ
                     GIIDNSKFAAFQDPRFDSLTEKEFEKVDIEISLLSKPVDCNLSDIKKGDGVIIRQGLA
                     TAVYLPQVWEQLPDKDSFLASLCEKAGLEWNCYKEKATKFQRFEVEIIE"
     gene            complement(2600..3307)
                     /locus_tag="PLK55_03865"
     CDS             complement(2600..3307)
                     /locus_tag="PLK55_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36090.1"
                     /translation="MLLKKIKNPFRRKTPKMPSKGYNTLTEAIKKTPEYTTKEVAIVK
                     VENEYYNISKSREKNRTIIDEKLLKKAFKHKKSKIVHTHTIGPLKELPSANDISVLFF
                     EHFKYKKNFTAISNIDRKTGEISGAIGVLFTTKTITFIENNFFIPKTRDVSILQRNIY
                     EAILKEQSAAKTRAQDIANFQKNKTGKTFIETYNKELKRIMFDYYQNVLGLKIRFVPN
                     TTKGYRFNKERMAFERI"
     gene            complement(3321..4706)
                     /locus_tag="PLK55_03870"
     CDS             complement(3321..4706)
                     /locus_tag="PLK55_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOZ36091.1"
                     /translation="MTTTLWIILVFAFAAFCATFARLVFKQRTTLWILILLDTKSLIK
                     RVNKLAEKHKKALTAMSVFGVAVGFGPFGVDYLIKDKATKKKRIFIFIISLLIATYIS
                     YLLAGSMFFGNPLIPKFISYLIIILTGIMGLSGFILGALLFSAYDIIAKMFVGKVACP
                     GIGLVIPGVKVPKTNFFIPWYGWLILIPAAFIHEFSHGIMLRVAKIKLKSIGVMLLGI
                     LPLGAYVEPDEEKFKKIDNKKKLKMYAAGPTSNLIVAIIFFIVYLLISAPIANYTTSI
                     DNQREIGLVVSQVTATTEVCGSSFDNPAFGKLVKNDLIISVNGNLIKSRNDLTKNIKK
                     DQENEFVVKNLDTNITRTEYITTNELGNIGITADVLVDETIKVPKDYSFYKILLSVIL
                     WVALLNFILATTNFLPTIPFDGGFMAQIIFCDFLNKKKNNEEKRMKKVMWFFICVLIF
                     LALLNIIPYFL"
     gene            4808..5848
                     /gene="fen"
                     /locus_tag="PLK55_03875"
     CDS             4808..5848
                     /gene="fen"
                     /locus_tag="PLK55_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HOZ36092.1"
                     /translation="MGVAIGSLIEDCKKRIDLSEFKGKKFGFDAYNMLFQFVTTIRGI
                     DGEPLKNENGEITSHYSGIFYRISNVLSAGIKPVFVYDGKSHELKAKTKQDRRDRREA
                     AKEKLEKAIVEEDIESMGKFARQSATIDSKIIDESKELLTAMGVSSFTAPGEAEAQIS
                     YMTAVGQTDYCVSQDFDCLLFGSPNLVKNIGVSGKRKVPFKKIYVDVYPYILESEQVF
                     SKLNITREKLIWMSLLIGTDFNDKVEGIGPKTALKLVQENNSFENILKQLKEKNKEID
                     FDYKEVIDIFLNPDIEKNPIYYKPIFDRQKIEHILIDKANFEEKRTINYLNEFEKKRN
                     EVDKQKTLSKWF"
     gene            5852..6238
                     /locus_tag="PLK55_03880"
     CDS             5852..6238
                     /locus_tag="PLK55_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36093.1"
                     /translation="MPTTKPKPKQVKRLAKKWFVETKPKVQFTKVLSQFPNVDKKEIA
                     LIKHGGAYTDISINRRKEVVTPEDYTSFFLRKSNPNDFKKEKILEKSGKFVKRLNAKE
                     EPISIHTHPMSDGVAFLMYNDINGNI"
     gene            6225..6644
                     /locus_tag="PLK55_03885"
     CDS             6225..6644
                     /locus_tag="PLK55_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36094.1"
                     /translation="MAIYNYFKSASSMKQNVSIELDKNGNQIGRTFFNVTKETALAAI
                     EMRGKNTDISFMNFLLKQIDLKYEEINLRFTQKYYGLFKKAKLKKDRDRILDQCNYEI
                     NSRLQRYFIDVLKIRFKFQAMPGYRYNNELMRFEKIK"
BASE COUNT         2632 a          975 c         1019 g         2055 t
ORIGIN      
        1 cttttactat ttgtatatgc ctaaaggaag ttccagcatc ggtagaaaac aaccaaaaag
       61 aataggcgac agtgttttta ttggaacacc acacggaaaa gaacttggct attctacaca
      121 tgccaaacaa atgattagag aaaacccagg actcttaaaa gcagtgtatg aattatcaaa
      181 aagtagaaaa ccatttttag aagacccaaa aagcagattt caaattttaa gacaaaaacc
      241 aaaagcaggc caaaacactg atgcctctta tattcttaaa ttaaaagaaa aaaaatacta
      301 tattaaagaa gtaaatgata ctagtataga tagagactat agttttgata ttaaacacaa
      361 acacggaaga gacggagtaa gtgaacatct agcaatagaa ttactaaaag aacaaggaat
      421 aaatgtcatt cctgcacatt tctcatatgt tgatccaata tctaaaaaaa gctttatagt
      481 ttatgaattc acacaactta gaactcttga tgagttatat aaaactggag aaattacttc
      541 tattagatat aaaaatataa aaaaacaagt aaaagaaata gaagaagaaa taaatagaaa
      601 atttcattct tttggattag ataaatataa acaaatctgg gatatacgta cagacaatgt
      661 tttctatagt acaggaaaaa ataaatttta tgtttttgat ccaattttat taaaaaaata
      721 aggaggtaaa taaatatggc aaaaggatgt atgttttgta agatagcaaa agggaaggtg
      781 ccaagctata agatatatga aaacaagaag gtgattgctt ttctggatat caatccaata
      841 gctaaaggac acacactagt gattccaaag aaacattttg aaaacgtctt tgattgtaaa
      901 gacaaaattc tagcagaaat gataaaagca acacaaaaaa ttgctcttca ctataaagag
      961 aaactaggat gtactggtgt aaatatccta aacgcttcag gagcttcagc agaacaacaa
     1021 gtaccacatc ttcatttcca cataatacca agaattgaag gagacggaca caagatttgg
     1081 ccagtcacag aacatgttac agaaaactta gaaagactag cagaagaaat aaaaatggaa
     1141 gacgaactag aaccaaagaa aaagaaaaaa acagaagaaa ataatggcta aaaagatttt
     1201 cttttccatt cttttttcat ttattatatt tggaataata atactaccag tatttttgga
     1261 gaaagacatg aatttaaaaa ttagaaaacc agcagtttct ggaacttttt atccttctag
     1321 tcaattagcc ttaacttctc aacttgaagg gttttttaaa gatacagaaa tagaatattc
     1381 tggggaaatt attaatgctt taattgctcc acatgctggt tatatctatt ctggaaaaac
     1441 cgcaatgttt ggttataaac aactctatat ggacctaatc ttaagaaaag aagacagctt
     1501 taaagtaata atattagcac caaatcacac agaatacaca aacaagataa taattccaga
     1561 ctataattcc tttagaaccc cacttggaga tattaaactt aattccacaa agataaaagc
     1621 agaaataaac aactctccac atgagcagga acacgcaatt gaggttcagc ttcctttttt
     1681 acaatatatc tttaagaaag caaataaaga atttacaatt attccaatta ttgttggaga
     1741 cgttaatgca gaagaaataa aggcattagc aaaagacata aactcagaaa ttacagaaaa
     1801 tacaatcata attgtctcaa gcgacctttc acactaccag ccacaagaga aagcacaaga
     1861 aattgataat aaaactatac acaacatctt ggacagaaaa acaaccgcaa gccttgacgc
     1921 ctgcggctct aacccaataa aggtactaga agagatatca atattaagaa gctggaaaga
     1981 accaaaactt ctaaactact caacttcagg agacgtcaca agagacaaga aggctgttgt
     2041 tggctatgct agtatatctt ttacagaaga aaaagaacat cttctcttaa gacttgcaca
     2101 cagaactata caaaacgcat ttgatgcaag aaaagaaaat ttttttgatt tagaacaatc
     2161 tgtacctaaa gaatacctag aacgaaaagg aagttttgta actctaacaa taaacaaaag
     2221 actaagagga tgtattggga atattaaagg cacaaaaacc gtatttcaag gaataattga
     2281 taactcaaag tttgcagcat ttcaagatcc tcgctttgat agcctaacag aaaaggaatt
     2341 tgaaaaagtt gatattgaga tttctttgct gtctaagcca gttgattgta atctttcaga
     2401 cataaagaaa ggagacggag taattattag acaaggacta gcaacagcag tttacctgcc
     2461 acaagtttgg gagcagttac cagacaaaga ttctttttta gcaagccttt gtgagaaagc
     2521 aggattagaa tggaattgct ataaagaaaa agccacaaag tttcaaagat ttgaagtaga
     2581 aataattgaa taaaagaaat tagattctct caaaagccat tctctcttta ttaaatctat
     2641 aaccctttgt agtatttggt acaaatctta ttttcaaccc aagaacattt tgataataat
     2701 caaacataat tctttttaat tctttattgt aggtttctat aaatgtttta ccagttttat
     2761 ttttttgaaa attagctata tcttgggctc tggtttttgc agcactttgt tctttaagta
     2821 ttgcctcata aatgttccgt tgtaaaatag atacatctct ggtttttggt ataaagaaat
     2881 tattttctat aaaagttatt gttttggtag taaataatac gcctattgct ccagatattt
     2941 ctccagtttt acgatcaata tttgaaattg cagtaaagtt ttttttatat ttgaaatgtt
     3001 caaaaaataa tacagatata tcatttgcag aaggtagttc ttttaatggt ccgattgtgt
     3061 gagtatgcac tattttggat tttttgtgtt taaatgcctt tttaagtaat ttttcatcaa
     3121 ttattgttcg atttttttct cgagatttgc ttatattata atattcgttt tctactttta
     3181 caatagctac ttcttttgtt gtatattctg gtgttttttt gattgcttca gttagtgtat
     3241 tatagccttt tgaaggcatc tttggtgttt ttcttctaaa aggatttttt atttttttta
     3301 aaagcatcta tttctcacca ttatagaaaa taaggaataa tatttaataa tgccaagaaa
     3361 attaatacac atataaagaa ccacattact ttcttcattc tcttttcttc attatttttc
     3421 tttttattta gaaaatcaca aaatataatc tgtgccataa acccaccatc gaaaggtatt
     3481 gttggaagaa agttagttgt tgcaagtatg aaatttaata atgcaaccca taaaataaca
     3541 cttaacaata ttttataaaa actataatct tttggaactt taatagtttc atcaaccaaa
     3601 acgtcagcag ttattcctat atttcctagt tcatttgtag ttatatactc tgttcttgta
     3661 atatttgtat ctaaattttt aacaacaaat tcattttctt gatctttctt tatatttttg
     3721 gttaaatcat ttctgctttt tataagattg ccattaactg agataattaa atcattttta
     3781 acaagcttac caaaggctgg attatcaaaa gaagaaccac aaacttcggt tgtcgcagtt
     3841 acttgactta caacaagacc aatctctcgt tgattgtcta ttgatgtggt atagtttgct
     3901 attggtgcag aaatcaataa atacactata aagaaaataa ttgcaacaat caggttactt
     3961 gtaggacctg ccgcatacat ctttagtttc tttttattgt ctattttttt aaatttttct
     4021 tcatctggct caacataagc accaagtggt aatatgccaa gtagcattac accaatggac
     4081 tttagcttta tttttgcaac gcgtagcatt attccatgac taaactcatg aataaaagcc
     4141 gcagggatta atatcaacca accataccaa ggaataaaga aatttgtttt aggaaccttt
     4201 acacctggaa taacaaggcc aatacctggg caagcaactt tccccacaaa catcttcgca
     4261 ataatatcat atgctgaaaa aagcaatgcc cctagaataa aaccagacaa tcccattatt
     4321 ccggttagta ttattatcaa ataactaata aatttaggaa ttaaaggatt accaaaaaac
     4381 atacttcctg ctaataaata agaaatataa gttgcaatca ataatgaaat aataaatata
     4441 aaaattcttt tcttttttgt agccttatct ttaattaaat aatctacacc aaaagggcca
     4501 aagccaacag caacaccaaa tacagacatt gcagttaatg cttttttatg tttttctgct
     4561 aatttattta cacgtttgat tagagatttt gtatcaagta aaattaaaat ccataaagtt
     4621 gttctttgtt taaatactaa tcttgcaaaa gttgcacaaa atgcagcaaa agcaaaaact
     4681 aaaataatcc ataaagtagt agtcactaat tatcccattc cttaaaagta tatttattga
     4741 tataatatat aaagtatatt atatttatat tagttatgtt atttaatata attgttggag
     4801 attaaaaatg ggagtagcaa ttgggtcttt aattgaagat tgtaagaaaa ggatagatct
     4861 ttctgaattt aaaggcaaaa aatttgggtt tgatgcctat aatatgctgt ttcagtttgt
     4921 aacaactatc agagggatag atggtgaacc tctaaagaac gaaaacggag aaattactag
     4981 tcattattca ggaatattct atagaatatc taatgtttta agtgcaggta ttaaaccagt
     5041 gtttgtttat gatggtaaaa gccatgaact aaaagcaaag actaaacaag atagaagaga
     5101 cagaagagaa gcagcaaaag aaaaactaga aaaagcaata gtagaagaag acatagaaag
     5161 tatgggcaaa tttgcaaggc agtctgcaac aatcgattca aaaattattg acgaatcaaa
     5221 agagttgtta acagcaatgg gagtttcgtc ttttacagca ccaggagaag cagaagcaca
     5281 gatttcttat atgactgctg ttggacaaac agactactgt gtctctcaag actttgattg
     5341 tcttttgttt ggttctccaa atcttgtaaa aaatatagga gtaagcggaa aaagaaaagt
     5401 tccgtttaaa aaaatatatg ttgacgttta tccatatatc cttgaaagcg agcaagtttt
     5461 ttctaaactt aatatcacaa gagaaaaact tatctggatg tctcttttga taggaacaga
     5521 ctttaatgac aaggttgagg gtattggccc aaaaactgct ctaaagcttg tacaggaaaa
     5581 caatagcttt gaaaacatct taaagcaact aaaagaaaaa aacaaagaaa ttgattttga
     5641 ttacaaagaa gttatagata tatttcttaa cccagatatt gaaaaaaatc caatctatta
     5701 taagcccatc tttgacagac aaaagataga gcacatactt atagacaaag caaactttga
     5761 ggaaaagaga acaataaact atctaaacga atttgaaaaa aaaagaaacg aagtagataa
     5821 acagaaaacg ttaagtaaat ggttttgaaa tatgccaaca acaaaaccta aaccaaagca
     5881 agtaaaaagg ctagcaaaga agtggtttgt agaaacaaaa ccaaaagttc aatttactaa
     5941 agtactttct cagtttccaa atgtagataa aaaagaaatt gcactaataa aacatggtgg
     6001 ggcatatact gatattagta taaatcgacg aaaagaagta gttactccag aagattatac
     6061 tagctttttc cttcgtaaat ccaatccaaa tgattttaaa aaagaaaaaa ttttagaaaa
     6121 atcagggaaa tttgtaaaaa gattaaatgc gaaggaagaa ccaatatcta tacacacaca
     6181 cccaatgtct gatggtgttg ctttcttaat gtataatgat ataaatggca atatataatt
     6241 attttaaatc agcatcttca atgaaacaaa atgtttctat agagctggat aaaaatggca
     6301 accagatagg aagaactttc tttaatgtga ctaaagagac tgctttagcc gcgattgaaa
     6361 tgcgtggtaa aaatacagac ataagtttta tgaatttttt attaaaacaa attgatctta
     6421 agtatgaaga aattaatctc cggtttactc agaaatatta cgggttattt aaaaaagcaa
     6481 aactaaaaaa agatagagat agaattctag atcaatgtaa ttatgaaata aattctagat
     6541 tacaaaggta ttttatagat gttcttaaaa tacgttttaa atttcaggcc atgccaggat
     6601 atagatataa taacgaatta atgagatttg aaaaaataaa ataagaaata ccaaagaaaa
     6661 caacagtttt ctgggctcag a
//
LOCUS       DAOOON010000036         6373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_14870_length_6373_cov_13.450301, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000036 DAOOON010000000
VERSION     DAOOON010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14870_length_6373_cov_13.450301"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PLK55_03890"
     CDS             <1..975
                     /locus_tag="PLK55_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HOZ36095.1"
                     /translation="LTDIVAYSVKSNPYITETLEEKTNSLFLIHHINELQHIKHINRI
                     WFMLHSPEKEELDILFQKGIDKFIIDNINDLNFLEKYITQENKQITLLLRMRLKEYTV
                     QTGRYFVYGFFSKDINELIPKLKQNKNIKELGIHVHRKTQNLSEWNHVEELEESLSPD
                     TWEAINYIDIGGGIPIDYKNVKAETIKDIYKKIKELKDFVNKRNIKLIIEPGRAIAGP
                     SGKLITTIKNIVDDNIFIDASVFNCAMDLIVANIKLLVENELEETNKQAKAYVIKGLT
                     PASEDIFRYRVYLKEPKVGDKIVFLNAGAYIYYTDLFNLERPKIEFEE"
     gene            1046..1282
                     /locus_tag="PLK55_03895"
     CDS             1046..1282
                     /locus_tag="PLK55_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36096.1"
                     /translation="MGVITISLDKKTESKLRDSAVLMYGAQKGAISKTITHAINQLEV
                     DKKGNNKKELFYLLDNPAKVKGTMPKHRSELYDK"
     gene            1272..1694
                     /locus_tag="PLK55_03900"
     CDS             1272..1694
                     /locus_tag="PLK55_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOZ36097.1"
                     /translation="MTNKILVDTNILVYAFTDIDLNRQKIAKELLKNLINNNESLAIS
                     IQNVVEFTKTTKVKFSKPITDERLDEVLSKFRINFDIISYDINTIKRISPILKESTDF
                     FDCLLVATMQDYDIDTIITENEKDFKKINNITIINPFK"
     gene            1701..2408
                     /locus_tag="PLK55_03905"
     CDS             1701..2408
                     /locus_tag="PLK55_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOZ36098.1"
                     /translation="MNKIIENLRKELKKSSDSKTKESSKRFFKEEIKVYGVKSAIVGQ
                     IAKKYYPQIKDLKKEEVFKICEELLKSDYCEESYIVADWLPREIKKLEKKDIYIFKIW
                     IEKYINNWAKCDTLCNHTIGDMITKYPELAKEVKSWSKSKNTWMKRASAVSFILPARR
                     GEYLDIAFEISDTLLLDKEDLVQKGYGWLLKEASRKHQKEVLSYVIKNKKIMPRTALR
                     YAIELMPKDLREKAMSK"
     gene            2414..3316
                     /locus_tag="PLK55_03910"
     CDS             2414..3316
                     /locus_tag="PLK55_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOZ36099.1"
                     /translation="MNFMNLPKEYRDKNKSRVVILPISFKTKTTRKNESHLGPKAIID
                     ASQELEYFEEDLLCEPHEVGIFTEEELKIKETDFIKIKHKILDKFSKVYDGKKFPVIL
                     GSDHSTTIPIIEALEKKEKDFGVIVFDAHSDLREPWGKDTWWHACVSREISKKHKTLL
                     VGTRSMDNTDLQFLQSRAGNNTGLIFAKDLLSSKKAHNWDLSHCFELEEKLRQLPKKI
                     YISIDVDVFDPAIIRYTDTPEPGGLNWTQINNLLKIIFTEKEVLGADIVEFSPEAKDK
                     PSFAESYLLSKLIYKIIAYKYYIK"
     gene            3325..3492
                     /locus_tag="PLK55_03915"
     CDS             3325..3492
                     /locus_tag="PLK55_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36100.1"
                     /translation="MITKKKKDYLIPIVIFIGIATLFFFPSPADHLLQYFAVMLFFVI
                     LIIFLIKRNKR"
     gene            3496..>4208
                     /gene="zupT"
                     /locus_tag="PLK55_03920"
     CDS             3496..>4208
                     /gene="zupT"
                     /locus_tag="PLK55_03920"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOZ36101.1"
                     /translation="MNVILTGLLLSILAGLSTAIGGALIFLIKDPKKSYLAFSLAFAT
                     GVMITVSFIELLPEGIHAIGYTNALLAFFIGSVFVFLVDYFTPHTYIMEKFSKNKEFN
                     PKLFKVGLLVALGIAIHNLPEGFAIFAGNLNSFKFGLLITVAIAIHNIPEGFAVSMPI
                     FYATKSRKKAFISSVYSGLAEPIGAILAAIILLPFLSEKIIGASLAFVAGVMVYICID
                     ELLPTAYSSCDGKQHTMTFA"
     assembly_gap    4209..4308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4383..4568
                     /locus_tag="PLK55_03925"
     CDS             4383..4568
                     /locus_tag="PLK55_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOZ36102.1"
                     /translation="MPAKDKTGPEGKGPGTGRGLGDCNPDNTSENKPFGFGRGCGCGR
                     GRGRGLGYRNQQATNKE"
     gene            4571..4846
                     /locus_tag="PLK55_03930"
     CDS             4571..4846
                     /locus_tag="PLK55_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOZ36103.1"
                     /translation="MPRRRCQRRIRFSPETIYFKPAGVTLKELEEINLRADELEAIRL
                     KDFEGFEQEKAASKMQISQPTLHRALLSARKKIADALVNGKAIRLEK"
     gene            4890..>6373
                     /locus_tag="PLK55_03935"
     CDS             4890..>6373
                     /locus_tag="PLK55_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36104.1"
                     /translation="MNTKKIFSFIFLILIIFNLGIVFAEDCETDPNADSCVIEDDSVF
                     NPFNNINYNNTQETNVLGIVYFTGIGCPHCAKIDPILLRDIALIRNDLAIIEYEVKAL
                     KEFNANLMLEYNEQYGSGTGYPLAIFSENDYYVSGNILTILEEYLRDGKKTNITLPES
                     VYEEGTTAFEKIDFSKLKGKPTIWYNRKVISRYYDGEIPIDDKILHQILEAKNIEEIE
                     NIIKQQEYHTIDDTKIQLSQTEVVFDNTIHIPGWKVYYNGNSTLNYVACPITEEVTDT
                     DCAEKKKIPLLTVVSLALVDAVNPCEFAVLIMLLIAIMTADPKNKKRTLYAGLAFTAA
                     IFLLYFIYGILLINIFKLIPAIDTIRKIVYTVIAAIAITIGVFQIKDFFFYKPGGFLT
                     EMPLSFRPKVKKLISSITSPKGAFFVGAFVTLFLMPCTIGPYVILGNLLSTETILVAL
                     PQLLLYNIIFIIPMLAITIIVYLGVKKVDEVASWKERNIKYMHL"
BASE COUNT         2524 a          853 c         1005 g         1891 t
ORIGIN      
        1 ctaacagata ttgtagctta tagtgtaaag tctaatcctt acattacaga aacacttgaa
       61 gaaaaaacaa attctttatt tctaattcat catattaatg aattacaaca tatcaaacac
      121 ataaacagaa tatggtttat gctacattct ccagaaaaag aagaacttga tattcttttc
      181 caaaaaggta tagataaatt cataattgat aatattaatg atctaaactt cttagaaaaa
      241 tatattacac aagaaaataa acaaataact ttacttctaa gaatgagact aaaagaatat
      301 actgtacaaa caggaagata ttttgtatat ggtttctttt ctaaagatat taatgaatta
      361 attcctaaat taaaacaaaa taaaaacata aaagaactag gaatacatgt acatagaaag
      421 acacagaatc tttcagaatg gaatcatgtt gaagaactag aagagtcttt atctccagac
      481 acctgggaag caatcaacta catagacatt ggtggaggaa taccaattga ttacaagaat
      541 gttaaagctg aaactataaa agacatctac aagaaaataa aagaactaaa agactttgta
      601 aacaaaagaa acataaaact aataattgaa ccaggaagag caattgctgg tccttctgga
      661 aagttaatta caactatcaa aaacatagta gacgacaaca tctttattga cgcttcagtt
      721 ttcaattgtg caatggatct tattgttgct aatattaagc ttcttgtaga aaatgaactt
      781 gaagaaacaa acaaacaagc taaggcttat gtcattaaag gattaactcc agcttcagaa
      841 gatatcttta ggtatagagt atatcttaaa gaaccaaaag ttggagacaa gatagttttt
      901 cttaacgcag gagcatacat ttactacaca gatcttttca atcttgaaag accaaagata
      961 gagtttgagg aataaacgaa agtatttaaa aatatttcga tttaaatata tatttatata
     1021 tttaaataaa agcaaggtgt gaaatatggg tgtaataaca atttctttag ataaaaaaac
     1081 cgaaagtaaa ttaagagact cagctgtact tatgtatggt gctcaaaaag gagcaataag
     1141 taaaactata actcatgcaa ttaatcaatt agaagtagat aaaaaaggaa ataataaaaa
     1201 agaattattt tatcttttag ataatcctgc aaaagtaaaa ggaacaatgc ctaaacatag
     1261 aagtgaacta tatgacaaat aaaattttag tagatactaa cattttagtc tatgccttta
     1321 ctgacattga tctaaataga caaaaaatag caaaggaatt attgaaaaat ttgataaata
     1381 ataacgaatc attagcaata agtattcaaa acgtagtaga atttacaaaa actaccaaag
     1441 taaaatttag caaaccaata actgatgaaa gattagatga ggtattatct aaatttagaa
     1501 ttaattttga tataataagt tatgatataa atacaattaa aagaattagt cctatattga
     1561 aagaatcaac agatttcttt gattgcctat tggttgcaac aatgcaagat tatgacatag
     1621 ataccattat tacagaaaat gagaaagatt ttaagaaaat taataatata actattatta
     1681 atccgtttaa gtgagtaatt atgaataaaa taattgaaaa tttaaggaaa gaacttaaaa
     1741 aaagttcaga ctcaaaaaca aaagaaagca gtaaaaggtt ctttaaagaa gaaataaaag
     1801 tgtatggggt aaaatccgca atagttggtc agatagctaa gaagtattat cctcagatta
     1861 aagatttaaa aaaagaagaa gtttttaaga tttgtgaaga attattaaaa tcagactatt
     1921 gtgaagaatc atatattgta gcagattggc taccaagaga aattaagaaa ctagaaaaga
     1981 aagatattta catatttaag atatggattg aaaaatatat taataattgg gcgaaatgcg
     2041 atactctttg taatcataca attggagata tgattactaa atatccagaa ctagcaaaag
     2101 aagtaaaatc ttggtcaaag tctaaaaaca cctggatgaa aagagcttca gcagtttctt
     2161 ttatccttcc tgcaagaaga ggagaatatt tagatattgc ttttgaaata tctgatactt
     2221 tacttttaga caaagaagat cttgtgcaga aaggctatgg ttggttatta aaagaagcaa
     2281 gtagaaaaca tcaaaaagaa gttcttagtt atgttataaa aaataaaaag ataatgccaa
     2341 gaactgcatt aagatatgca attgaattaa tgcctaaaga tttaagagaa aaagcaatga
     2401 gtaagtgatt gttatgaact ttatgaatct tccaaaggaa tatagggaca agaataagtc
     2461 aagagttgta atacttccaa tttcctttaa gacaaagacc acaagaaaga acgaaagtca
     2521 tcttggccca aaggcaataa ttgatgcttc ccaagaacta gaatactttg aagaagattt
     2581 actttgtgaa ccacatgagg ttggcatctt tacagaagaa gaactaaaga taaaagaaac
     2641 agactttata aagattaaac acaaaatcct ggacaagttc tcaaaagtct atgatggaaa
     2701 gaaatttcca gttatccttg gctcagacca ctcaacaact attccaatca tagaagcact
     2761 tgaaaagaaa gaaaaagatt ttggagtaat tgtgtttgat gcacactctg acttaagaga
     2821 accctggggg aaagacactt ggtggcatgc ttgcgtgtca agagagattt caaagaaaca
     2881 taaaactctt cttgttggaa caagatcaat ggataacaca gacctacagt ttcttcaatc
     2941 aagagcagga aataacacag gactaatatt tgctaaagac cttctttcct caaagaaagc
     3001 acataactgg gatctttctc attgctttga gttagaggaa aagctaaggc aattaccaaa
     3061 aaagatttac atttcaatag atgttgatgt ttttgaccct gcaattataa gatacacaga
     3121 cacaccagaa ccaggaggtc ttaactggac gcaaattaac aatcttttaa aaataatctt
     3181 tacagaaaaa gaagtgcttg gagcagacat tgtagagttt tctccagaag ctaaagacaa
     3241 accctctttt gcagaaagct atcttctttc aaagctaata tataagatta tagcatataa
     3301 gtattatata aaataagaaa caatatgatt acaaaaaaga aaaaagacta tctaatacca
     3361 atagttatat ttattggaat tgcaacattg ttcttttttc caagtcctgc tgatcatctc
     3421 ttacaatatt ttgcagtaat gcttttcttt gtaatattaa taattttttt aataaaaagg
     3481 aacaaaagat agaacatgaa tgtaattctg acaggactat tattatctat tttagcaggg
     3541 ctatctactg caattggtgg agctttaata tttttaataa aagacccaaa aaaaagttat
     3601 ttagctttct ctttagcatt tgcaacagga gtaatgatta ctgtttcgtt tatagaatta
     3661 ctacctgaag gaatacatgc aataggttac actaatgcac ttcttgcttt ctttataggt
     3721 tctgtatttg tttttcttgt agattatttt acaccacaca catatatcat ggaaaaattt
     3781 tcaaaaaata aagaattcaa tcctaaattg tttaaagttg gtttactggt ggcattagga
     3841 attgcaatac acaacttacc agaaggcttt gcaatatttg caggaaatct aaactctttt
     3901 aagtttggac ttttaataac tgttgcaatt gcaattcaca atattccaga aggctttgct
     3961 gtatctatgc caatatttta tgctacaaaa agtagaaaaa aagcatttat ttcttcagta
     4021 tattctggtc ttgcagaacc aattggtgca atcttagctg caattatatt attgccattc
     4081 ttatcagaaa aaataattgg tgcaagcctt gcttttgttg caggagttat ggtttatatt
     4141 tgtatagatg aattactacc aacagcatat tccagttgtg atggtaaaca acacaccatg
     4201 acttttgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa tgaatattta
     4321 ttcaaaacga tctgtttaaa aacctttttg aatatatatt cattatgagg aggcgataaa
     4381 aaatgccagc aaaagataaa acaggtcctg agggaaaagg cccagggaca ggaagaggcc
     4441 taggagattg taatccagat aacacttcag aaaataaacc atttggtttt ggaagaggtt
     4501 gtggttgcgg aagaggccgt ggaagaggtt taggttatag aaatcaacaa gcaacaaata
     4561 aggaataaat atgccaagaa gaagatgtca aagacgaata agatttagcc cagaaactat
     4621 atactttaaa ccagctgggg taactctaaa agaattagag gaaataaatt tacgagcaga
     4681 cgaacttgaa gcaatcagac taaaagactt tgaaggcttt gagcaagaaa aagcagcaag
     4741 taaaatgcaa atatcccagc cgactcttca tcgtgcgctt ttaagtgcaa gaaaaaagat
     4801 agctgatgct ttagttaatg gaaaagccat aagacttgaa aagtaatctt tatatttgtt
     4861 tttgaaaata aataatatag gtaatagcta tgaacacaaa gaaaatattt agttttatat
     4921 ttctaatttt aataattttt aatttaggca ttgtatttgc agaagactgc gaaacagatc
     4981 caaatgcaga cagttgtgtt atagaagatg attctgtctt taatccattt aataatataa
     5041 attataataa tactcaagaa acaaatgttt taggaatagt atattttaca ggaattggtt
     5101 gcccacattg tgcaaagata gacccaattc ttttaagaga tattgcatta attagaaatg
     5161 atcttgcaat tatagaatat gaagtaaagg cattaaaaga atttaatgca aatctaatgt
     5221 tggaatataa tgaacaatat ggttcaggaa caggctatcc acttgcaatt ttttcagaaa
     5281 atgattatta tgtttcagga aatattctta caatcttaga agaatattta agagatggaa
     5341 agaaaacaaa tatcacttta ccagaatcag tttatgaaga aggaactact gcttttgaaa
     5401 aaatagattt tagtaaacta aaaggaaaac caaccatctg gtataataga aaagtaattt
     5461 ctagatatta tgatggagaa ataccaattg atgacaaaat actacatcag atattagaag
     5521 ccaaaaatat agaagaaata gaaaacataa ttaaacaaca agaatatcat acaattgacg
     5581 atacaaaaat acaattatcc cagacagaag ttgtttttga taatacaata catatacctg
     5641 gttggaaagt atattataat ggtaatagca cacttaatta tgtggcttgt cctataacag
     5701 aagaagtaac agatacagac tgtgcagaaa agaaaaaaat tcctctctta actgttgttt
     5761 ccttagcttt agttgatgca gttaaccctt gtgaatttgc agttttaatt atgcttctta
     5821 tagcaataat gacagcagat ccaaaaaaca agaaaagaac cctatatgcg ggcctagcat
     5881 ttactgcagc aatattttta ttatatttta tttatggtat tcttttaatt aatatattta
     5941 aacttatacc agcaattgat accattagaa aaattgttta cacagtgatt gctgcaatag
     6001 ctataactat tggagtattc cagattaaag acttcttctt ttacaagccg ggaggattct
     6061 taacagagat gcctttatca tttaggccaa aagtcaagaa actaatatca agtattactt
     6121 ctccaaaagg tgcattcttt gttggggcat ttgttactct tttcttaatg ccatgcacta
     6181 ttgggccata tgtgatactt ggaaatctcc tttccacaga aacaatcctt gttgctcttc
     6241 cgcaattgtt attatacaat ataattttca taataccaat gcttgctata acaatcatag
     6301 tatatcttgg agtaaagaaa gtagacgaag tagcaagttg gaaagaaaga aacatcaagt
     6361 atatgcatct aat
//
LOCUS       DAOOON010000037         5494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_17887_length_5494_cov_17.275418, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000037 DAOOON010000000
VERSION     DAOOON010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17887_length_5494_cov_17.275418"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLK55_03940"
     CDS             complement(<1..413)
                     /locus_tag="PLK55_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36105.1"
                     /translation="MEHRLCLLNSSYEKDRARVVLYFGRDPLNNNYELIHKATFYPYF
                     VVEIEKELISKLLSDFKKDISLKSLEKKTKIIARNFELLSKCSKILQQATGKNIILIE
                     PERQFLIRNNWSYYDSFYVFKGNIRKINNENHIHFA"
     gene            589..1146
                     /locus_tag="PLK55_03945"
     CDS             589..1146
                     /locus_tag="PLK55_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36106.1"
                     /translation="MALEDLVKTMPKYTGIKEIIMNLNNYDDIFSDFDKKPYTHRSIS
                     DDFFAQLKKDCLEGDYKKLDLKIIVQKKNRNPRFEIIIRRRIEEHFKNHFKKLEKERK
                     RLVKLGIIFLIFGAILLIFKYILAEGLNNSQFKILSIVLEPFSWFLFWEGAYILFFET
                     KGNRIEYEIYKKLSKAKIQFISNDY"
     gene            1161..1619
                     /locus_tag="PLK55_03950"
     CDS             1161..1619
                     /locus_tag="PLK55_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36107.1"
                     /translation="MFDIKKVFILFSLVLILGTISAEGYMIDELFNNPEFAKEQYNAN
                     LDKMPGALRKFTGNEKIQLNVDYGTEIKSITLVMKDGAMDSYSTDGTVEPTLIVDTYP
                     QTIDNLIKSNTPVNDFKTAVKEKTLKYRSVGFFKKIKFGFARFALNFFKQ"
     gene            complement(1629..1798)
                     /locus_tag="PLK55_03955"
     tRNA            complement(join(1629..1663,1760..1798))
                     /locus_tag="PLK55_03955"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1761..1763),aa:Trp,seq:cca)
     gene            1904..2899
                     /locus_tag="PLK55_03960"
     CDS             1904..2899
                     /locus_tag="PLK55_03960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ36108.1"
                     /translation="MKLKYSGFLSITDNNQKTNIDQNKCSDISDYNLITHGHSDHIST
                     SQQQTFTTKETAALINMCTQKELNYSFVNGQALDLTDNIKLSSLDAGHILGSKMFYLE
                     TKDKSILYTGDFKTQDSLLFDGAKPKEADILIMETTFGKKEFVFPEREKVYEDFAEKI
                     SKDINNGKLVVIGAYSLGKSQEIIAFVNKYLKETPIITKKGFDFSKVYDSFEISLGNY
                     ELLNHNTDSHNILIVPMNLITKDLIRSLEHQQNRKVSVYFATGWKHYGSGKCIPLSDH
                     CDYNDLLDFVRAVNPKQVLTMHGFAEDFSKTINKELGIWSRPIEDLNQFSISDFL"
     gene            complement(2902..3429)
                     /locus_tag="PLK55_03965"
     CDS             complement(2902..3429)
                     /locus_tag="PLK55_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36109.1"
                     /translation="MAFNLKNIFSSKKATTREEENKNFNAIPVLEKTNKIDLATVISR
                     RATMNVDKLIDLTKVNEEIEKYKNMMMDYKKRGATERQLENLKEKIEELVIERQKMEV
                     LMELLNQDNLVKTAFGKKRQAEIHNTLLNRYRHGDMNLVDFKENLSELSLILKSRTDD
                     RNETQKLEDFNARLF"
     gene            complement(3480..4865)
                     /locus_tag="PLK55_03970"
     CDS             complement(3480..4865)
                     /locus_tag="PLK55_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOZ36110.1"
                     /translation="MTRNKYEYYNSDFHKIIENINKKNLTIKDISELVIKNHPTDKSQ
                     IIKDLLKLNNIEKIIYKNEIIEEEKKQLYKFLEKNGSDYIINYFMGYRQLTKLFEQKN
                     IEEIIINDYDKVFIIDREQKKTLTGITFKEKEYDKLVDVFKNTVKRDFKKREFIDGSL
                     PDRSRLNIVSKAVCGFNAITIRKFTKKPLTIIDLIKSEVLDAYNAAYLWTVIEGFKVK
                     PANLFVCGGTGSGKTTFLNVLMDFISDNERIILIEDTAEIDISNFNDSVSLISDISNE
                     ESLYEITINTLRMRPDRVIIGEVRAREVQGLFVAMDTGHEGCMATIHANNSEDAIIKL
                     LNKPMEIPETALPLLDLIVILKKKFVSGKTVRKLSQITEISKVGNINLNNIYKDDECK
                     DCSVDIMTSHFAEKLCNLVSISKTEFKKIIDYRAGLFEQICKTKPDISREDLKTILHS
                     PELDYLKILKK"
     gene            4971..>5494
                     /locus_tag="PLK55_03975"
     CDS             4971..>5494
                     /locus_tag="PLK55_03975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOZ36111.1"
                     /translation="MAERRDIFNEINSNKLKTIFLFIGFFAFIFLLAIAIKYIFNFTG
                     FGILFIAGVIAIIYAVIGYFVGDKIVLSTSGAKEADEKKYVYLINLVEGLSLSAGIPK
                     PKVYVIEDKSPNAFATGRDPKHSSLAVTTGLLEIMNRQELEGVIAHEMSHIHNRDIQV
                     MTFVSVLFGIVSIAS"
BASE COUNT         1835 a          790 c          799 g         2070 t
ORIGIN      
        1 gcaaaatgta tgtggttttc attatttatt tttcttatat tgcctttaaa aacataaaac
       61 gaatcatagt aactccagtt atttctaata aggaattgtc tttctggttc tattaatata
      121 atattttttc cggttgcttg ttgaagtatt ttagaacact tagaaagaag ttcaaagttt
      181 cttgctataa tttttgtttt tttctcaagg gacttaagag aaatgtcttt tttaaagtct
      241 gaaagcaact tggatataag ttctttttct atttctacaa caaaataagg atagaatgtg
      301 gctttatgta ttagttcata gttattatta agagggtctc taccaaagta gagaaccact
      361 ctagccctgt ctttttcata actactatta agtaaacaca atctgtgctc catggttatt
      421 attcaactcc acaatttcta aaatatcttg gcagataaag atatagtgtt ttaacaaaga
      481 gggggcattt tcgggggggt aggtgtccag tgcaagtttt ttaaagcttt atttttaatt
      541 aaaacaccat ttaaaactct tttggtctta agggttatag tgatttatat ggcactagaa
      601 gatttagtaa aaacaatgcc caaatatacc ggcataaaag aaatcataat gaatttaaat
      661 aattatgacg atatattttc agattttgat aaaaagccat atacgcacag atcaatttct
      721 gatgattttt ttgcacagct aaaaaaagat tgtcttgaag gagattataa aaaattagat
      781 ttaaagatta ttgttcaaaa gaaaaatcga aatcccaggt ttgaaattat aattagaaga
      841 agaatagaag agcattttaa gaaccatttt aagaaattag aaaaagaaag gaaaagatta
      901 gtaaaattag gaattatttt tcttatattt ggtgcaattt tattaatttt taaatacatt
      961 cttgcagaag gtctaaataa ttcgcaattt aaaattctgt caattgtctt agagccattt
     1021 agttggtttc tattttggga aggagcatat attttatttt ttgaaactaa aggcaataga
     1081 atagaatatg aaatatataa aaaattatcc aaagccaaga ttcagtttat aagtaatgat
     1141 tattaaaagg aggaaaaaac atgtttgata taaaaaaagt atttattttg ttttctttag
     1201 ttttaatttt aggaactatt tcggcagaag gctatatgat tgatgagtta tttaataacc
     1261 ctgaatttgc aaaagaacaa tataatgcaa atctagataa gatgccaggg gctttaagaa
     1321 agtttactgg taatgagaag atacaattaa atgtagatta tggcacagaa ataaaatcca
     1381 taactctagt catgaaagac ggagcgatgg attcttattc tacagacggc acagttgagc
     1441 caactttaat tgttgacact tacccacaaa caatagataa tcttattaaa agcaacacac
     1501 cagtaaatga ttttaaaaca gcagtaaaag aaaagacctt aaaatatcgt tcagttggtt
     1561 tttttaagaa aataaagttt ggctttgcaa gatttgcgct aaatttcttt aaacaataag
     1621 agaatatttg gggacactgg gatttgaacc caggtcaaca ggtatctcct ttttttctag
     1681 atatacatct agcatacctg taattgtcag tcatctgttt tttagaaaca tcctgcaaaa
     1741 tctttttttt gaaaaaaatc tggagcccgc taggctaacc ggactacccc atgtccccgt
     1801 tttgggtata taatttatat taaaaaatct ttatatacat gtgcttttta aaacaaaaca
     1861 aaagcaatat aaaataatca cgagatatta tttattgcta aacatgaaat taaaatattc
     1921 tggttttcta tcaattactg ataataacca aaaaacaaat attgaccaaa ataaatgttc
     1981 agatatctct gactataacc tgattactca cgggcatagc gatcatatct ctacaagcca
     2041 acagcagaca tttactacaa aagaaactgc tgctttaatc aatatgtgca cacaaaaaga
     2101 actaaattat tcgtttgtta atggacaagc actagattta actgacaaca ttaaactttc
     2161 ttctcttgat gctggtcaca tccttggcag taagatgttt tatctagaga ctaaagacaa
     2221 atctattctt tacactggag actttaagac acaagattct cttttgtttg atggtgcaaa
     2281 gccaaaagag gcagatatac taataatgga aacaaccttt ggtaaaaagg aatttgtgtt
     2341 cccagaaaga gaaaaagtct atgaggattt cgcagaaaag atttcaaagg acatcaataa
     2401 tggaaaatta gttgtaattg gagcatattc tcttggtaaa tctcaagaga taatagcgtt
     2461 tgtaaacaaa tatctaaaag aaacaccaat aattacaaag aaaggctttg atttctctaa
     2521 agtctatgac agttttgaaa taagtcttgg aaattacgag ctcttaaatc acaatacaga
     2581 ttctcacaat attttaattg ttccaatgaa tctaataact aaagatttaa taagaagtct
     2641 tgagcatcaa cagaacagaa aagtatcagt ttattttgca actggttgga agcattatgg
     2701 ttcagggaaa tgcatacctc tttctgacca ttgtgattat aatgatcttc ttgattttgt
     2761 aagagcagta aatccaaagc aagttctaac aatgcatggt tttgcagaag atttctcaaa
     2821 aaccataaac aaagaattag gtatttggtc aagaccaata gaagacctta accaattttc
     2881 aatttctgat tttttataat tttaaaacaa cctagcatta aaatcttcta acttttgtgt
     2941 ttcgtttctg tcatcagttc tggattttaa tattaatgaa agctctgaaa ggttttcttt
     3001 aaagtcaaca agattcatat ctccgtgtct atatctgttt agaagggtgt tatgtatctc
     3061 tgcttgtctt ttctttccaa atgcagtttt aacaagatta tcctgattta atagttccat
     3121 taagacttcc attttttgtc tttcaataac aagttcttca atcttttctt ttaggttttc
     3181 cagttgcctt tctgttgctc ctcttttctt gtaatccatc atcatgttct tgtatttttc
     3241 aatttcttca ttgactttag taagatcaat aagcttatca acattcattg ttgctcgtct
     3301 agaaattact gttgctaaat caatcttgtt tgttttttca agaacaggaa tagcattgaa
     3361 gtttttattt tcttcttctc ttgttgttgc ctttttacta ctaaatatgt tttttaaatt
     3421 aaatgccatt tttattcacc atatatacct tatagtttgt taatatttaa taaatctatt
     3481 tattttttta atatttttaa ataatctaat tcaggactat gtaatattgt ttttagatct
     3541 tctcttgaga tatctggctt tgttttacat atctgctcaa ataacccggc tctatagtct
     3601 ataatcttct taaattcggt tttagaaatg cttacaagat tacaaagttt ttctgcaaag
     3661 tgagaggtca taatgtctac tgaacaatct ttgcattcat catctttata gatattatta
     3721 agattgatat ttccaacctt agatatttca gtaatttgag aaagctttct tactgtttta
     3781 ccagaaacaa attttttttt taaaattact atgagatcaa gaagggggag agcagtttct
     3841 ggtatttcca ttggtttatt tagtaattta ataattgcgt cttcactatt attagcatgt
     3901 attgttgcca tacacccttc gtggccagta tccattgcca caaacaatcc ttgtacttct
     3961 cttgctctta cttctccaat aattactctg tctggcctca ttcttaaggt atttattgtt
     4021 atttcatata agctttcttc attagagata tcagagataa gagaaacaga atcattaaaa
     4081 ttgctaatgt ctatttcggc agtgtcttct attagtatta ttctttcatt atcagatata
     4141 aaatccatta aaacatttag aaaagtagtt tttccagaac cggttccgcc acacacaaaa
     4201 agatttgcag gtttaacctt aaatccttca ataactgtcc aaaggtatgc tgcattataa
     4261 gcatctaaaa cctcgctttt gattaaatct attatggtta atggtttttt tgtaaatttc
     4321 ctaatagtta ttgcattaaa tccacagact gcttttgaaa caatatttaa tctagaccta
     4381 tctggtaaag acccgtctat aaattctctt tttttaaagt ctctttttac tgtgttttta
     4441 aagacatcaa caagtttatc atattctttt tctttaaagg ttattccggt tagtgttttt
     4501 ttttgttctc tatcgataat aaacacttta tcataatcat taattattat ttcttcaata
     4561 tttttttgtt caaatagttt ggttagttgt ctataaccca taaagtaatt tattatataa
     4621 tcagacccgt ttttttctag gaatttataa agttgttttt tttcttcttc tattatctcg
     4681 tttttataaa ttattttttc aatattattt agttttaaaa ggtctttaat tatttgagat
     4741 ttatctgtgg ggtgattttt aataaccagt tcagagatgt cttttatagt taaatttttt
     4801 ttgtttatgt tttctataat tttgtgaaaa tcagaattat aatattcata tttatttcta
     4861 gtcataatgt ccccattttg tgctattatt tttaagatgt atttattttt aaatgttatt
     4921 attgttctat tatatatcaa acaagtacac cttttaataa aagataaatt atggcagaaa
     4981 gaagagatat ttttaatgag attaatagta ataaattaaa aaccatattc ctatttattg
     5041 gtttttttgc atttatattt ttattagcta ttgccataaa gtatatcttt aattttactg
     5101 gttttggaat actttttatt gcaggcgtta ttgcaataat ctatgcagtc attggttatt
     5161 ttgtaggaga caagattgtt ctttcaacaa gcggcgctaa agaagcagat gaaaagaaat
     5221 atgtttatct tattaatcta gttgaaggtc tttctctctc tgcaggaatc ccaaaaccta
     5281 aagtctatgt tattgaggat aagtccccaa atgcttttgc tacaggaaga gacccaaagc
     5341 attcaagtct agcagtaacc actggtcttc tagaaattat gaataggcaa gagctagaag
     5401 gcgtaattgc tcacgagatg tctcacatac ataacagaga catacaagtt atgacttttg
     5461 tgtctgttct ttttggtatt gtttcaattg cttc
//
LOCUS       DAOOON010000038         5275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_18830_length_5275_cov_12.167625, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000038 DAOOON010000000
VERSION     DAOOON010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18830_length_5275_cov_12.167625"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..624
                     /locus_tag="PLK55_03980"
     CDS             139..624
                     /locus_tag="PLK55_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36112.1"
                     /translation="MSITPQQLNPKLFNIKEKDGLTLITPKKQELSSEQIVVFAEHKG
                     KKYLAIAPKTETKGLNIHFKYREIDLLSFVLNRIKTRSIKEKPEMYLGTFFQNQSKIN
                     WQSKPLNKEAEAAIVLHLNKTFEQVIKKANAEKVRYEKEFQKELKKKAAKIPKRQLRR
                     L"
     gene            646..1407
                     /locus_tag="PLK55_03985"
     CDS             646..1407
                     /locus_tag="PLK55_03985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ36113.1"
                     /translation="MKQYSYDESSKYYDLLETSDKENKERNDFLEKAFKKYKITSVFD
                     LTCGTGAQAIYFLKKGYSVKASDFSKGMVDVANLKLKKENLKQRFVKGDMRDISFGKA
                     EAVITMFNAIGHLSKKDFIKTLGNIKENLKPKGYYIFDIYNLKDMPRDLPLKKHIDII
                     KEKENTILVRFCENKLNLKTGILHTKHELLIQHNLEGYKTIKSHWDMQLYDFKEIKGI
                     LERNDFKILEVYGGADRSTFKDKTSQSIYLITQKQ"
     gene            1537..2463
                     /gene="pyrB"
                     /locus_tag="PLK55_03990"
     CDS             1537..2463
                     /gene="pyrB"
                     /locus_tag="PLK55_03990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOZ36114.1"
                     /translation="MKEIKHIISIHDLEKEQINYILEKAKDIEDYPNKYIDALKGKVI
                     ASLFFEPSTRTKMSFETAVKKLGGNTIGFEDIQNTSAKKGETFSDAIKVIAGYSDLVV
                     MRTKIEGASRLASEIANKPIINGGDGTNQHPTQTLLDLYTIKKEKGKLEGLSIGFFGD
                     LKYGRTVHSLVEAISLYSPKKIKFIAAPQFQIGKEYLEMLDSKGIKYEILEDAKGNLK
                     DLDILYVTRTQLERIVEPIDMDPLKKKYTITKALLDGENNPGLLVMHPLPRVYEITTD
                     VDDTPHNIYFKQADNGVPVREAIICILLKVIK"
     gene            2460..2903
                     /gene="pyrI"
                     /locus_tag="PLK55_03995"
     CDS             2460..2903
                     /gene="pyrI"
                     /locus_tag="PLK55_03995"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOZ36115.1"
                     /translation="MSERKVGTINNGVVVDHITPGKGTEISKILGLDSLGEFVVIASN
                     LESKKSGKKDLIKIEGKELSQEELNKIALISKDSTINIIRNSSVFKKYPVVVPDIVEN
                     ILMCNNPYCISNNEKISSKFYCLDKSKVTLKCHYCEKKQNEIKIK"
     gene            complement(2907..3365)
                     /locus_tag="PLK55_04000"
     CDS             complement(2907..3365)
                     /locus_tag="PLK55_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36116.1"
                     /translation="MLKKKDRQKAVNELIRVAKKNAPIFVSVMGKYAILLSAIKRWPN
                     EVKDTVNFKRLSIDGDDYNWGSIKSSEKNKSFCHFFTVDELEKLFPKNKTKLLETVGL
                     EGFASAQEKEFAELKDKTIQKNWLEAHNLLCTTRTAADISSHMLIIVKKK"
     gene            complement(3346..3762)
                     /locus_tag="PLK55_04005"
     CDS             complement(3346..3762)
                     /locus_tag="PLK55_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ36117.1"
                     /translation="MTITKIKNVTKSVENYYDKSTEYEWNRLVRDKYHKLEWDTTQIF
                     LKKHLPKKGLILDAGAGPGRYTVDLAKKGYDIILLDYSKGNVELAKIKVKEAKQEKKV
                     KDFIQGTIVNLSQFKDNSFDAVICLGGPLSHVEKKR"
     gene            3834..4856
                     /locus_tag="PLK55_04010"
     CDS             3834..4856
                     /locus_tag="PLK55_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOZ36118.1"
                     /translation="MSEIYFKAINSYSNTEEINKTVLELFKKINPGFTGNLAIKVHFG
                     EEGNKTFISPKNYEKLINYIKLNRGSPAYIETNVLYKSKRTSKDSHIQLAREHGFTNL
                     PIIIADGEVGEEFIEVEINKKHFQKCKVGKAYGDFNQLIVISHFKGHGLSGFGGAIKN
                     LGMGFAARAGKLAMHSNSKPIINPLKCKKCHLCEQNCPVNAITIGLIPHIDTKKCIGC
                     AKCISICPHHACTVNWISTLPGTFEEKLCEYALAATKANKDVKIIYINFLLNITDNCD
                     CMSHTKIVAKDIGILAGTDPVALDKACYDFLNKKENKKIFGGEKTFDYAESIGLGTKE
                     YILKEI"
     gene            complement(4860..>5275)
                     /locus_tag="PLK55_04015"
     CDS             complement(4860..>5275)
                     /locus_tag="PLK55_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36119.1"
                     /translation="WWDSLLHTFSGFALGLVVFGAMYVLYKTQKIKTSAIFIAFIIFS
                     ITMTAGVIWEIYEYSYDNIAGGNMQRARNLCPETGFCDSRLGLMDTMKDFLLNGIGAI
                     FASILGYIYLKKGEHFLLNGMIKRFVENNPKLFYK"
BASE COUNT         2058 a          766 c          837 g         1614 t
ORIGIN      
        1 catacaagaa aaagatagat attcacaagt atgctaaaga cccaaagcac aaagaactaa
       61 gaagggcaat tgagttcttt gaaagacaga aaaataaaaa ttcagagaaa attacaaaga
      121 aaaaaaaaag gtgaaaatat gagtattacg ccgcaacaat taaatccaaa attgtttaat
      181 ataaaagaga aagatggact aacattaata actccaaaaa agcaagagct ttcttctgag
      241 caaattgttg tttttgcaga acataaagga aaaaagtatt tagcaattgc tccaaaaaca
      301 gaaacgaaag gattaaatat tcattttaaa tatagagaaa ttgatttact ttcttttgtt
      361 ctaaacagaa taaaaacaag aagtataaaa gaaaaaccag aaatgtatct tggcacattc
      421 tttcaaaatc aaagcaaaat aaattggcag tctaaaccac ttaataaaga agcagaagca
      481 gcaatagtat tacatttaaa taaaactttc gagcaagtaa ttaaaaaagc aaatgcagaa
      541 aaagtaagat atgaaaaaga atttcaaaaa gaattaaaaa agaaagcagc aaagatacca
      601 aaaagacaac taagaagact atagtatatc aaagtgaaaa acaaaatgaa acaatacagc
      661 tatgatgagt ctagtaaata ttatgatttg ctagagacaa gcgacaagga aaataaagaa
      721 agaaatgatt ttcttgaaaa agcctttaag aaatataaaa ttacttctgt ttttgatttg
      781 acttgtggaa ctggtgctca agcaatctat tttctaaaga aaggctatag cgttaaagcc
      841 agtgacttta gtaaaggaat ggtggatgtc gctaatctta agttaaaaaa agaaaatcta
      901 aaacaaagat ttgtaaaagg ggatatgcga gatatttctt ttggtaaagc agaagcagtt
      961 ataacaatgt ttaatgctat tgggcatctt tctaaaaagg attttataaa aactctagga
     1021 aatattaaag agaatcttaa accaaaaggc tactatatct ttgatatcta taatctcaag
     1081 gacatgccaa gagatctgcc tttaaaaaag cacatagata ttattaaaga aaaagaaaac
     1141 acaatattag taagattctg cgaaaataaa cttaatctta agactggtat tttacacaca
     1201 aagcatgaac ttttgattca gcataatctt gaaggctata aaaccattaa aagccattgg
     1261 gatatgcaac tttacgactt taaagaaata aaaggaatac ttgaaagaaa cgactttaaa
     1321 attcttgaag tgtatggtgg agcagacaga agtaccttta aagacaagac tagccagagc
     1381 atctacttga ttactcaaaa gcaatagaaa atatttttaa atacttcgct gctttatatt
     1441 taagaatgaa aaaattaacc aaaaatttat tttttggaaa tcgtctcttt cttatcgaag
     1501 ttattttttt ctgtctattt ataagggggt aaaactatga aggaaataaa gcatataatt
     1561 tctatacatg atcttgaaaa agaacaaata aactatattc tagaaaaagc aaaagatata
     1621 gaagactatc caaacaaata tatagatgcc ttaaaaggaa aagtaattgc aagtcttttc
     1681 tttgagccat ccacaagaac caagatgtct tttgagactg ctgttaagaa gcttggagga
     1741 aacacaatag gctttgaaga tatacaaaac acttctgcaa aaaaaggcga aacctttagt
     1801 gatgcaatta aagttatagc agggtattca gatttagtag tcatgcgaac aaagattgaa
     1861 ggggcttctc gtcttgcttc tgaaattgca aacaaaccaa taatcaatgg aggagacggc
     1921 acaaaccagc atccaacaca aacacttctg gatctttata ccattaagaa agaaaaagga
     1981 aaacttgaag gactatcaat tggttttttt ggagacctaa agtatggtag aacagttcat
     2041 tctcttgttg aagcaatatc tctttatagt cctaagaaaa taaaatttat agctgctcct
     2101 cagtttcaga ttggtaaaga gtatcttgaa atgcttgaca gtaaaggaat aaagtatgaa
     2161 atcttagaag acgcaaaagg gaacttaaaa gacctagata tcctttatgt aacaagaaca
     2221 caacttgaaa gaattgttga accaatagat atggacccat taaagaagaa atacacaatc
     2281 actaaagcac ttcttgatgg agaaaataat cctgggcttc tagtcatgca tcctttgcca
     2341 agagtctatg agataacaac tgatgttgat gatactccac acaacattta ctttaagcaa
     2401 gcagacaatg gagtaccagt tagagaagca ataatatgta ttcttttaaa ggtgataaaa
     2461 tgagtgaaag aaaagtagga acaataaaca atggagtagt agttgaccac ataactccag
     2521 ggaaaggaac agagatttct aaaatccttg gccttgattc tttgggagag tttgtagtta
     2581 ttgcaagtaa tctagaaagt aagaaatcag gaaagaaaga cttgattaaa attgaaggaa
     2641 aggaattaag tcaagaagag ctaaataaga ttgcacttat ttcaaaggac tcaacaataa
     2701 acataattag aaactcaagt gtgtttaaga aatatccagt tgtagtccct gacattgtag
     2761 aaaatatttt gatgtgcaac aatccttact gtataagcaa taacgagaaa attagcagta
     2821 aattctattg ccttgacaaa agtaaagtaa ccctaaagtg ccattactgc gaaaagaaac
     2881 aaaatgaaat aaagataaaa taatctttat ttctttttaa caataattag catatgactt
     2941 gaaatatctg ctgcagttct tgttgtacaa agtaagttgt gtgcttctag ccaattcttt
     3001 tgtatagtct tatcttttaa ttcagcaaat tctttctctt gagcagaagc aaatccttca
     3061 aggccaacag tttctaataa ctttgtttta ttttttggaa ataatttttc tagttcatca
     3121 actgtaaaga aatggcagaa agatttgttt ttttcagagc ttttaataga tccccaatta
     3181 tagtcgtctc catctattga taatctttta aaattaacag tatctttaac ttcattaggc
     3241 catcttttta ttgcactaag aagtatagca tattttccca taactgaaac aaatattggt
     3301 gcattcttct ttgcaacccg tataagttca ttaactgctt tttgtctatc tttttttttc
     3361 aacatgagat aatggccctc caaggcagat tactgcatca aaggaattgt ctttaaattg
     3421 agagagattt acaattgttc cttgtataaa atctttaact ttcttttctt gttttgcttc
     3481 tttaacttta atttttgcaa gttctacatt tcctttagaa taatctaaga gaataatatc
     3541 ataacctttt tttgctaagt caacagtata tcttccagga ccagctcctg catcaagtat
     3601 taatcctttc tttggaagat gttttttaag gaatatttga gtggtatccc attctagttt
     3661 atgatattta tctcttacaa gtctattcca ctcatattct gtagatttat cataatagtt
     3721 ttctacagat tttgtaacat ttttaatttt agtaattgtc atatataatc acaattattt
     3781 atgaatcaat tattttataa tattatttgt aatctatatt atagtggtaa gttatgtcag
     3841 aaatatattt taaagcaatt aatagttatt caaatacaga agagattaat aaaactgttc
     3901 ttgaattatt taaaaaaatt aatccagggt ttacaggaaa cttagcgatc aaagtgcatt
     3961 ttggagaaga aggaaacaaa acttttattt ctccaaagaa ttatgaaaaa ctaatcaatt
     4021 atattaaact aaatagaggc agtccggctt acattgaaac aaatgtttta tacaaaagca
     4081 aaagaacctc taaagatagt cacatacaac tagcaagaga acacggcttt actaatcttc
     4141 caataattat tgcagatgga gaagttggag aagagtttat agaagtagaa attaataaaa
     4201 agcattttca gaaatgtaaa gttggaaaag cctatggtga ttttaatcaa ttaatagtta
     4261 tttctcattt taaaggacat ggactttcag gttttggtgg agcaataaaa aatctaggta
     4321 tgggttttgc tgcaagagca ggaaaacttg caatgcattc taactctaag ccaattatta
     4381 atccactaaa atgtaaaaaa tgccatctct gtgaacagaa ttgtcctgtt aatgctataa
     4441 ctattggatt aattcctcac attgacacaa agaaatgtat tggctgtgca aaatgtattt
     4501 ctatttgtcc acatcatgct tgtactgtta attggatatc tactcttcca ggaacctttg
     4561 aagaaaagct ttgcgagtat gctttagctg caacaaaggc aaacaaggat gttaaaatta
     4621 tctatattaa ctttttacta aacattacag acaactgcga ctgtatgtct cacacaaaga
     4681 ttgtggcaaa agacataggc attcttgcag gaactgatcc tgttgctctt gataaagctt
     4741 gttatgattt tttaaacaaa aaagaaaata aaaaaatatt tggtggagaa aagacctttg
     4801 attatgcaga aagcattggt cttggaacaa aagaatatat cttaaaagaa atataaaatt
     4861 tatttataga ataacttagg attgttttct acaaatcttt taatcattcc attcagaaga
     4921 aaatgttcgc ctttttttag atagatataa cctaagattg aagcaaagat tgcgccaata
     4981 ccattaagca aaaaatcctt catggtgtcc attaatccta gacgagaatc gcaaaaacca
     5041 gtttctgggc aaagatttct tgctctttgc atatttcctc cagcaatgtt atcatagctg
     5101 tattcatata tttcccagat tactcctgca gtcattgtta tactaaaaat aataaaagca
     5161 ataaagattg cactagtctt tatcttttgc gtcttataca agacatacat tgcgccaaaa
     5221 actacaaggc ccaaagcaaa tccagagaag gtgtgaagta atgaatccca ccacc
//
LOCUS       DAOOON010000039         4938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_20464_length_4938_cov_18.861765, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000039 DAOOON010000000
VERSION     DAOOON010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20464_length_4938_cov_18.861765"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..731
                     /locus_tag="PLK55_04020"
     CDS             48..731
                     /locus_tag="PLK55_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36120.1"
                     /translation="MNKKTIISLVILGIIIIGGIFYFYKTRVNKINLNNIENNEESNQ
                     ELISEKNEDSNFENEIVLNNEIQVEINFTKDAFTVGETPENAYYFVDYKDKNPLDVLV
                     LFGKTKEGLVKKSYGKYRGNLDSIFKTIISEKYDCDGNYIYYLSVYDCNSVDKLLATE
                     KCRGIRDEKDLKNIDQNLDTLYFIQKEITVNCPLENPSCCSNMAYSCIYDNDCLTGYY
                     CQDGDCLKN"
     gene            792..1736
                     /locus_tag="PLK55_04025"
     CDS             792..1736
                     /locus_tag="PLK55_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36121.1"
                     /translation="MNKLSVFVMVIIVSALITPFIFASTSIITPADSSQNNYYVSNYS
                     TREISFSVNYDFKSEELKDGKIVYSYKVLPNSEGLDIQLSNTEILFTNNFSNTETIYL
                     NTKQLTEIKNLQIKSEVFDIYNNLLFTKVLYLKIIPNNSNNYYEYTKTHSEPRYAGYS
                     ISRTIAVINGLKDYDIISIYTKTEDNSPLALSCFTSNQAIVVEQQYQSPERTDIKLAI
                     DSKAKDLKSGDYYISCKLFNAYVSQELPEIKLRYTSYNISKETEDNQNQSVSITETAK
                     SVTGFLSFSKAKSSNFTYVLFIILIILILLILFSRNSS"
     gene            complement(1733..2176)
                     /locus_tag="PLK55_04030"
     CDS             complement(1733..2176)
                     /locus_tag="PLK55_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOZ36122.1"
                     /translation="MNKINHILFASILFVIFYMFFADILKITGPNTFAAYVICALYSL
                     IPDLDLKSSWIKGQFNQFVLYAIVILGIIYLFVTPETTILIIIGILVLFALLLFLMKH
                     RDVLHTPLIGVLFAIPLLFINYPGIAYFLSGCIGILSHWLLDKIT"
     gene            2275..3084
                     /locus_tag="PLK55_04035"
     CDS             2275..3084
                     /locus_tag="PLK55_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOZ36123.1"
                     /translation="MDLIAYILLGVIQGIFEWLPVSSQGISVVVLMNMFKVAPQLAID
                     MSIFLHLGTVLAALVYFWKDIKELFTKTNYSNLFKLKKNIKFDNTTQVSRFILVSVFF
                     TLLIGVPIYFLVRQNVTAEMVGILTIAIGVLLIITGLLQIKINKAREAIPKFTTKDAF
                     WVGGAQGLSVIPGVSRSGITTSMFLFQGHNPEIAFKYSFLLSIPTILIAEIGLLVING
                     FIFNPLILVSVAMAFIFGFITIDILMKIARKLEFSYFCFVLGLAYILIGLI"
     gene            3096..3416
                     /gene="trxA"
                     /locus_tag="PLK55_04040"
     CDS             3096..3416
                     /gene="trxA"
                     /locus_tag="PLK55_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOZ36124.1"
                     /translation="MSESILHLTDKNFDEEIKKNKFIVVDFWAEWCGPCRMFSGTYEE
                     YAKENKDVSCAKINIDQAPVTSEKYGVMSIPSILIFKEGKLVAQKTGALPKVMLKAFV
                     DSFK"
     gene            3426..>4938
                     /locus_tag="PLK55_04045"
     CDS             3426..>4938
                     /locus_tag="PLK55_04045"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOZ36125.1"
                     /translation="MSYKKKLTVKILDIDADKPIALLHSKTASDLLAVTQNRVEIKFN
                     GTTKIFILDITDSMIDEDTIGIYKDVTGVTKLKTGDKVEVEVVPSPSSVHHIVKKIRN
                     NPLTEEEITEIINDIVDNRLSDIEISAFLTAVTINGINLEETTNVCKAMTATGNVIEF
                     NVQQVLDKHSIGGINGRVSMIVTPIITSLGYVMPKTASRSISSAAGTADCMEVLADVS
                     FDIKDIKKMVSSVGGMVAWNGKVDLCPADDKMIKIRHSLGLDPEGLVIASVLSKKKSV
                     SATHLIIDIPIGPTVKVRNKEDGERWANKFSRICKEIGIKSKVVLTNGEVPCGRYFGA
                     ALEAKGALEVLENKYFDNFAEKSCEIAGCLLELVERIEPGKGYDLAKDTLKSGKALSK
                     FKEMLKAQNGKIFSSEEIPYANFKQEILATDGGKIVGMNVSNLTRIARTSGAPHDKLA
                     GIILNKEVGEKINPGELIFTIYSNSQDKLKIATELAKSTMTDTIKIEKIILEEV"
BASE COUNT         1877 a          650 c          801 g         1610 t
ORIGIN      
        1 tataaagcta ttgtcctaat tatttatata aatttaaagg tgattaaatg aataaaaaaa
       61 ctataatttc tttagtaatt ttagggatta ttataatagg aggtattttt tatttttata
      121 aaactagagt aaataaaata aatttaaata atatagaaaa taatgaagaa tctaatcaag
      181 aattaatctc agaaaaaaac gaagattcta attttgaaaa tgaaatagtc ttaaataatg
      241 aaatacaagt agaaataaat tttacaaaag acgcatttac tgtaggagaa actccagaga
      301 atgcttatta ttttgtagat tataaagata aaaacccatt agacgtattg gttttatttg
      361 gaaaaactaa agaagggctt gtaaaaaaat cctatggaaa atatagaggc aacttagatt
      421 caatatttaa aacaattata tcagagaaat atgattgtga tggaaactat atatattatc
      481 tttcagttta tgattgtaat agtgttgata aattattagc tactgaaaaa tgtaggggaa
      541 tacgagacga aaaagattta aaaaatattg atcaaaattt agatacatta tatttcatac
      601 aaaaagaaat aaccgttaat tgcccactag aaaatccgtc ttgttgttca aatatggctt
      661 attcttgtat ctatgataat gattgtttaa ccggatatta ttgtcaagat ggagattgct
      721 taaaaaatta atttcattaa tttctggggg aatccatacc tttataagga ttaactgcct
      781 agaattatat aatgaacaaa ctatctgttt ttgttatggt aattatagtt tctgcactta
      841 taactccgtt tatttttgca agtactagca taataacgcc tgcagattct tcacaaaaca
      901 actattatgt ttctaattac tcaacaagag aaataagctt tagtgtaaat tatgatttta
      961 aatcagaaga actaaaagac ggaaagatag tttatagcta taaagtttta ccaaactcag
     1021 aaggcctaga catacaacta tcaaacactg aaatattatt tacaaacaac ttttcaaata
     1081 cagaaactat ttatcttaat acaaaacaat taactgaaat taaaaatctt caaattaagt
     1141 cagaagtctt tgatatctac aataaccttt tattcactaa agttctatat ctaaaaatta
     1201 taccaaacaa ttccaataac tattatgaat atacaaagac acattctgaa ccaagatatg
     1261 ctggttattc aatttcaaga acaatagcag taattaatgg tttaaaagat tatgatataa
     1321 tttctatata cacaaaaaca gaagataatt ctcctctagc actatcttgc tttacaagta
     1381 accaagcaat agtagttgag caacaatatc aaagtccaga aagaacagat ataaagctag
     1441 caattgattc taaagcaaag gatttaaagt caggagatta ttatatttct tgtaagcttt
     1501 tcaatgctta tgtttctcaa gaactaccag agattaagtt aagatatact agttataata
     1561 tttctaaaga aacagaagac aatcaaaacc aaagcgtttc gattacagaa acagcaaaat
     1621 cagtaactgg gtttttaagt ttttcaaaag cgaagtctag taattttact tatgttcttt
     1681 ttattattct tataatttta atattattaa ttcttttttc tagaaatagt tcttaagtaa
     1741 tcttatccag taaccaatga gaaagtattc caatacatcc agataagaaa tatgcaatgc
     1801 ctggataatt aataaataat aaaggtatag caaataatac tccaataaga ggcgtgtgta
     1861 aaacatctct atgcttcatt aagaataaaa gcaaagcaaa caatacaagt attccaatta
     1921 ttattaatat tgttgtttct ggagttacaa acaaatagat tattccaaga ataacaatag
     1981 catagagcac aaactgatta aattgtccct ttatccagga acttttaaga tctaaatctg
     2041 gtattaaaga atataatgca caaataacat atgccgcaaa ggtgtttggt ccggtaattt
     2101 taagtatgtc tgcaaagaac atataaaata ttacaaacag aatgcttgca aacaatatgt
     2161 gattgatttt gttcattata atatcttttt ttaaaccttt tataaatatt atatatgttt
     2221 aaagctttat atttgttttg tattataatt ttactagggg attagtgaaa aattatggat
     2281 ttaattgcat atattctttt aggtgttatt caaggaatct ttgagtggtt accagttagt
     2341 tctcaaggca tttctgtagt tgttttaatg aatatgttta aagttgcgcc acaactagca
     2401 attgatatgt ctatattttt acatcttgga actgttcttg cagctttagt atatttttgg
     2461 aaagacataa aagaactatt tacaaaaaca aattattcta atctttttaa attaaaaaaa
     2521 aacataaaat ttgataatac cactcaagtt tctagattta ttttagtttc tgttttcttt
     2581 actttgctta ttggcgttcc aatatatttt ttagttagac aaaatgtaac tgcagagatg
     2641 gttggtattt taactattgc aattggtgtg cttttaataa ttactgggct attacaaata
     2701 aaaataaata aagcaagaga agcaattcct aaatttacaa caaaagacgc attctgggtt
     2761 ggaggagcac aaggtctttc tgttatccca ggggttagta gatctggaat aacaactagt
     2821 atgttcttgt ttcaagggca caatccagaa attgctttca agtatagttt tttattatct
     2881 attcctacaa ttttaatagc agaaatcggg ttactagtaa ttaatggttt tatatttaac
     2941 cctttgattt tagtttcagt agctatggcc tttatttttg gatttataac cattgatatc
     3001 ttgatgaaga ttgcaagaaa actggaattt tcttatttct gttttgtttt aggtttagca
     3061 tatattttaa ttggtttaat atagggggaa aataaatgag tgaaagtatt ttacatctaa
     3121 cagacaagaa ttttgatgaa gaaattaaaa agaacaaatt tattgtggtg gatttctggg
     3181 cagaatggtg tgggccatgt cgtatgtttt caggaactta tgaagaatac gcaaaagaaa
     3241 acaaagatgt gtcttgcgca aagataaaca ttgaccaagc accagtaact tcagagaaat
     3301 acggtgttat gtcaattcca tcaattctaa tttttaaaga aggaaagcta gttgcacaaa
     3361 agactggcgc tcttccaaaa gtaatgctaa aagcatttgt tgattctttt aaataggaat
     3421 aatttatgtc atataaaaaa aaactaactg ttaagatttt agatattgat gcagataagc
     3481 cgattgcttt acttcacagt aaaactgcaa gtgatcttct agcagtaaca cagaatcgcg
     3541 tagagattaa atttaatggc acaacaaaaa tatttattct ggatattaca gactcgatga
     3601 ttgatgaaga tactattggt atttataaag acgtaactgg cgttacaaaa ctaaaaactg
     3661 gagacaaagt tgaagtagaa gtcgtaccat ctccgtcttc tgttcatcac atagttaaaa
     3721 aaataagaaa taatccttta acagaagagg aaattacaga aataattaat gatattgttg
     3781 ataatagatt atcagatatt gagatttctg catttttaac tgcagttaca attaatggaa
     3841 taaatcttga agaaaccaca aacgtttgta aagcaatgac agccactggc aatgtaatag
     3901 aatttaatgt gcaacaggtt cttgataagc actcaattgg tggaataaac ggtagagttt
     3961 ctatgattgt tactccaatc attacatctc taggctacgt tatgccaaag accgcttcaa
     4021 ggtcaataag ttcagcagca ggaacagcag actgtatgga agttctagca gatgtttctt
     4081 ttgatattaa agacattaag aaaatggttt cttcagttgg aggaatggtt gcttggaatg
     4141 gtaaggttga tctttgtcca gccgatgata agatgattaa aattaggcat agtctaggac
     4201 tagaccctga aggcctagtc attgcttctg ttctttcaaa aaagaaaagc gtttcagcaa
     4261 cacatcttat tattgatata ccaataggcc caacagtaaa agtaagaaac aaagaagacg
     4321 gagagcggtg ggctaataag ttttcaagaa tatgtaaaga gattggtata aaatcaaaag
     4381 ttgttctaac aaatggagaa gtaccttgcg gcagatattt tggtgctgct cttgaagcaa
     4441 aaggcgcact tgaagttctg gaaaacaaat actttgataa ctttgcagaa aagtcttgtg
     4501 agatagcggg ctgccttctt gagcttgttg aaagaatcga gccaggtaaa ggctatgatt
     4561 tggctaaaga cacactaaaa tcaggtaaag ctttaagtaa atttaaagaa atgttaaaag
     4621 cacagaatgg caagatattt tcttcagaag aaataccata tgcaaatttt aagcaagaaa
     4681 tcttagcaac agatggggga aagattgttg gaatgaatgt ttctaatctt acaaggattg
     4741 caagaacctc tggtgctcca cacgacaagc ttgcagggat aattctaaac aaggaagttg
     4801 gagaaaagat taatcctgga gagctaatct ttacaatcta tagtaacagc caagacaaat
     4861 taaagattgc gacagagctt gcaaaatcca caatgacaga cacaatcaaa atagaaaaga
     4921 taatacttga agaagtta
//
LOCUS       DAOOON010000040         4753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_21441_length_4753_cov_17.111750, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000040 DAOOON010000000
VERSION     DAOOON010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21441_length_4753_cov_17.111750"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLK55_04050"
     CDS             <1..1035
                     /locus_tag="PLK55_04050"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOZ36126.1"
                     /translation="KNQVFISRLRSLNISEITNPYNSEEKLIENISYDRNFSLKVAGV
                     DSGFVSKQLNFANITIIKECGMFFDYQNGELKDYKYFPKMYNLPKPYLSSSSLELEEI
                     MWNTSISRLEKELEISSLILGQTEKTLDLILFDGSIIPQYINKPTKDCPQKKKYEHLL
                     SRFLEVYKEARNKKIFLAGTIEDCRANRFFSILKDEIFNDQNLDFELFDAFLVFSLVS
                     KNQRTCVFKYSKDLKSHPILSDFPEEIRDNLYCCYLKLSDNDYPLRLEFVYYKEFGLS
                     LKDYTDKLVKMVSEISSFNQKYTYPSVLIEADIRSRLTVQEIDLITNKILEKTRSYGF
                     RLPRRESRVF"
     gene            1409..1864
                     /locus_tag="PLK55_04055"
     CDS             1409..1864
                     /locus_tag="PLK55_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36127.1"
                     /translation="MTNNDTTDTKRIFLEQLLGNQFKELGLSDLMAIQEKTQDPLVLS
                     VLVFKLLEERKETNRILKEIQEKYDKLQTSIQTKENTNKNLEIVLLPETDDRIISYIK
                     EHGKADAEEIKEMFGYKGTNAASQRLNNLVKANYLQKVNSGRKVYFVVK"
     gene            2108..2440
                     /locus_tag="PLK55_04060"
     CDS             2108..2440
                     /locus_tag="PLK55_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36128.1"
                     /translation="MSIQRLGGIAFLIGLFLCIIFAFLSPAVWITITLLLLGLIVGFL
                     NIKDKDTNQFLLASIVLIATSATPLGELPAIGGFLQNLVINFASFVGPAALIVSIFVI
                     VKIAHIKF"
     gene            2567..4735
                     /locus_tag="PLK55_04065"
     CDS             2567..4735
                     /locus_tag="PLK55_04065"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOZ36129.1"
                     /translation="MKNKNSILYIVIGILLVGVIAAFFIGANTNKQAPVTTGPNTNTP
                     TTNITTNNTTTQTQPASFKGSDFLVLTKENLLLLLQQGSTGSSGGRETKMLSVDSAVM
                     NEAAAPTAMESSDSTVTNGSSDYSGTNVQVKGIDEGDIVKTNGEKIFIGKTHKLIVIN
                     NVNPPLDDIYDLEISKEITNYQYYTNPEETIQTILLDEDLLYLVTTKYHTESKFDNSL
                     LYPQRLSIPTTVVTTYEIKEDSLEELSKIEIDGIYYQSRLKEGYVYLITNNYNFYYNN
                     PILLDDVIINPIIETKAGKVSSLDRTIIMPKDGTPENTNMYTVTTLKYSEASDSVIDS
                     LDLLLDYSSTIYMSEDNLYIAHKENQYWGWRWYGFYNNNMEVFKEIYKDVYPSSVENE
                     IKENIDDSEKILEILNKYYETLDEKEKEELYNDIQEETEKYYQNKQREREKTYINKIE
                     LKSDGKFGDITTGSVQGNLLNQFSLDEDKDGYLRVAITYRNENYKNENGIVILDSSLK
                     EYSTLDGLAEDESIYSVRFMGDKVFLVTFRQMDPFFVIDLSDRKNPGVLGYLKIPGYS
                     SYLHPISDTLILGVGQNTKATQWGGVSQTGVKVSLFDVSDYEDPKEVATYVVDTDNSY
                     TPIQNDHKAFLYIEKENLIVIPVTEYYNTRGPGMNFYVLELKDDDIVEKEIIKHGGNN
                     SWYSGILRSLYIGDELYTISDNQISTYNFDTEDKTKVSLN"
BASE COUNT         1834 a          721 c          736 g         1462 t
ORIGIN      
        1 aagaaccaag tctttatttc taggcttcgt tctcttaata tttctgaaat tactaatcct
       61 tataattctg aagaaaaact tattgaaaat atttcttatg atagaaactt ttctcttaaa
      121 gttgcaggcg ttgattcagg gtttgtgtca aagcaactaa attttgcaaa cattacaatc
      181 ataaaagaat gcggaatgtt ctttgattac cagaatggag aattaaaaga ctataaatat
      241 ttcccaaaga tgtataatct ccccaagcct tatctttcta gttcttctct tgagcttgaa
      301 gaaatcatgt ggaatacttc aatttcaaga ctggaaaaag aactagagat ttcctcacta
      361 attcttggac aaactgaaaa aacccttgac cttatattat ttgacggctc aatcattcct
      421 caatacatca acaagccaac aaaggattgt ccacagaaaa agaaatacga gcatttgctt
      481 tcaaggtttt tagaagttta caaagaggca agaaacaaaa agattttctt agcaggaaca
      541 attgaagact gtagagccaa tcgtttcttt tcaatcttaa aagatgaaat atttaatgat
      601 cagaatttag attttgagtt atttgatgca tttcttgttt tttctttagt ttctaaaaac
      661 caaagaactt gtgtttttaa atattccaag gatcttaaat ctcatccaat cttaagtgat
      721 ttccctgaag agataagaga taatctctat tgttgttact tgaagctttc agataacgac
      781 tatcctcttc gtttggagtt tgtgtattac aaggagtttg gtctatctct aaaagactac
      841 acagacaagc ttgttaaaat ggtgtctgag atatcttctt ttaatcaaaa gtacacctac
      901 ccttcagttt taatagaggc tgacatcaga agccgcttga cggttcaaga aatagatctt
      961 attacaaata agatactaga gaaaactaga agttatggct ttaggctgcc aagaagagag
     1021 tcaagggttt tctaggattt gtttttttaa ctgtttaggc ctttatttta caaaaaacaa
     1081 acacaatctt ttattaacat aatctataca cactactact gaaatatata aataatacgg
     1141 aaattggtgg tagatatgat gcttttggat gttctggaat tctcaggagt actaacggct
     1201 tgcgttgttt gtacaatata cttagcaaga aacaaaattg gactataaat actaaatttt
     1261 tgctaaaaaa cgcaaaattt gctaaaaatc aagcaatttt tgctaaaaaa ccccccttgg
     1321 ttaggaaatc taaccaaaat ctctcttttt ctaaaaaaat tgaaagcgat ccttttaaaa
     1381 caaagtcatg actatatcat gatatactat gacaaataat gacacaacag atactaaaag
     1441 aatattcctt gagcagttgt taggaaatca attcaaggag ttagggttat ctgacttaat
     1501 ggcaattcag gaaaagactc aggatccatt agttctttca gttctagtat ttaagttact
     1561 agaggaaaga aaagaaacta atagaatcct aaaagagatt caagagaaat acgataaact
     1621 acaaacatcc atacaaacca aagaaaacac aaacaaaaac ctagaaattg tccttttgcc
     1681 agaaacagac gataggatta tcagctatat taaggagcac ggaaaagctg atgctgaaga
     1741 aatcaaagaa atgtttggtt acaaaggaac aaatgctgca agtcagagat taaataacct
     1801 agtaaaagca aattacttac aaaaagtcaa ctctggaaga aaggtttatt ttgtagtaaa
     1861 ataaattaaa aagtggaaaa atagattttt tagaccccca atctaagaat ttggtaataa
     1921 agaaaaacta caaaaagaac ttttccttcc ttttttctgc ctaaagtaat taatagttat
     1981 aaaaaacaaa agagaaagag attttcgatt cttttctctt ttctgcctat ttttgaaaat
     2041 aaaacttatt taaaaacatt tttatttcat atattaatat ttatattaaa ttttgggggt
     2101 tttaaaaatg agtattcaaa gacttggagg aattgccttt ctaataggtt tatttctgtg
     2161 tattattttt gcttttcttt caccagcagt ctggataaca ataaccttgt tacttctagg
     2221 tttaattgtt ggttttttaa atattaaaga caaagacaca aatcagtttt tgctagcttc
     2281 aattgttctg attgcaacaa gtgcaacacc acttggagag ttacctgcaa ttggcggctt
     2341 tctacaaaac ctagtaatta actttgcaag ttttgtaggt ccagcagcgt tgattgtttc
     2401 aatctttgta attgttaaga ttgctcacat aaaattctaa aagagcaatt tcttttttct
     2461 tttttctttc tttaggtaaa aacctaaata ataaaaccct tttataatgt atttgtatct
     2521 attatttatc tatacttaat ataaaattaa aaatggtgga aaatatatga aaaataaaaa
     2581 ttcaattctc tatatagtaa taggaatatt attagttggt gtgattgctg ctttctttat
     2641 tggcgcaaat accaataagc aggcaccagt aacaactggc ccaaatacca atactccaac
     2701 aacaaatata acaacaaaca acacaacaac ccaaacacag ccagcttctt ttaaaggctc
     2761 tgatttctta gtattaacta aagaaaacct attgcttctt ttacagcaag gctcaacagg
     2821 ttcaagcggg ggcagagaaa caaaaatgct ttctgtagac tcggcagtaa tgaatgaagc
     2881 agcagctcca acagcaatgg agagttctga ttcaacagta acaaatggtt cttcagacta
     2941 ttctggaaca aatgttcaag taaaaggtat tgatgaagga gacattgtta agacaaatgg
     3001 agaaaagata tttattggta aaacccataa attaatagtt ataaataatg taaatccacc
     3061 acttgatgac atatatgatt tagagatatc aaaagaaata actaattatc aatattatac
     3121 aaaccccgaa gaaacaattc aaacaatatt actagacgaa gatctattat atttagtaac
     3181 cacaaaatac catacagagt caaaatttga taattcttta ctttatcctc aaagattatc
     3241 tattccaaca acagtagtaa caacttatga aattaaagaa gatagtctag aagagttaag
     3301 taaaatagaa attgatggaa tctattacca atcaagacta aaggaaggct atgtctatct
     3361 tataacaaat aattataatt tctattataa caatccaata ttattagacg acgtaataat
     3421 aaacccaata atagaaacaa aagcaggaaa agtttcaagt cttgatagaa caataattat
     3481 gccaaaagac ggaactcctg aaaacacaaa catgtacaca gttactacat taaagtattc
     3541 agaagcatct gattctgtaa ttgattcttt agatctttta ctagattatt ctagcacaat
     3601 ctacatgagt gaagacaatc tttacattgc tcacaaagaa aaccaatact ggggttggag
     3661 atggtatggt ttctataata acaatatgga agtctttaaa gaaatctaca aagacgtcta
     3721 tccaagttct gtagaaaatg agataaaaga gaatatagat gattctgaaa aaatattaga
     3781 aattctaaat aaatattatg aaacattaga tgaaaaagaa aaagaagaac tatataatga
     3841 cattcaagaa gaaacagaaa aatattatca aaataagcaa agagaaagag agaagactta
     3901 tatcaataag atagaactaa aatcagatgg taagtttgga gatataacaa caggttctgt
     3961 ccaaggaaat cttctaaatc aattttcttt agatgaagac aaagatggtt atttaagagt
     4021 agcaataact tatagaaatg aaaattacaa aaacgagaac ggaatagtta ttcttgattc
     4081 ttctctaaaa gaatattcaa ctcttgacgg tcttgcagaa gatgaaagca tctatagtgt
     4141 aaggtttatg ggagataaag tgttcttagt aacctttaga caaatggatc ctttctttgt
     4201 tatagactta agcgacagaa aaaatcctgg agtcttaggt tatctaaaga ttccaggata
     4261 cagtagttac ttacatccaa taagcgatac tttaatactt ggcgtcggtc aaaacacaaa
     4321 agccacacag tggggtggag tttctcaaac aggtgtaaag gtctcattat ttgatgtctc
     4381 agattatgag gatccaaaag aagtagcaac ctatgttgtt gacacagaca actcctacac
     4441 accgatacaa aacgaccata aggctttcct atacatagaa aaagaaaact taattgttat
     4501 accagtaaca gaatattaca atacaagagg tccaggaatg aatttctatg ttctagagtt
     4561 aaaggacgac gatatagttg aaaaagaaat tataaagcat ggcggaaaca atagttggta
     4621 tagtggtatt ctaagatcct tatatatcgg agacgagcta tacacaattt cagacaacca
     4681 gatatcaacc tataactttg acacagaaga taaaacaaaa gttagcctta actaactttt
     4741 tctttttctt att
//
LOCUS       DAOOON010000041         4615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_22229_length_4615_cov_25.550877, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000041 DAOOON010000000
VERSION     DAOOON010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22229_length_4615_cov_25.550877"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLK55_04070"
     CDS             complement(<1..419)
                     /locus_tag="PLK55_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOZ36130.1"
                     /translation="MTILNFKQYSLDIPARVYLPSDDTDLMIEVLDAEIIKSNKTFDS
                     AIELGSGNGFLSLEIYDNVKEIISTDINPIVIDYLLNVKEQYNLEKQKITYSNLFDSI
                     DEKKKFDLIIFNPPYVPSEEILSEDDDEINGYDLAVNG"
     gene            429..1175
                     /locus_tag="PLK55_04075"
     CDS             429..1175
                     /locus_tag="PLK55_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOZ36131.1"
                     /translation="MENKDLKFIDIHCHYDELSLECLKETFSKEEMIAIANNVDIESY
                     RKLERLRKENIPGLYFAYGLYPDVVVKVGLKDSLNYLKIIFENSKNILAIGEIGLDYK
                     ITKDLEIRKEQKIVFEKQLEIAKERNLPVIIHSRYATKAVLDVLSGYSGFPIILHWFS
                     GNEKEIQTALDRDYYLTLRFNKPSIPNIKEHLNHIFIETDYPISYDGKPLEITDIKKS
                     YEIFAKENNIDLEYLKEKINNNFNKVFLRR"
     gene            1188..1799
                     /locus_tag="PLK55_04080"
     CDS             1188..1799
                     /locus_tag="PLK55_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36132.1"
                     /translation="MIKKNSEIYDLLIAWLAISLCFTIVLGNYSLLDYLTTKQFNFTW
                     ADFFTKFGLSLIITSTSFIFHELAHKYMGIHLGAHGRFIMWPVFLIAGVALAGLLGVV
                     FVAPGAVYIFGKQHNIKEDGIISLAGPATNLIMALLFFIAALFGAPILIVSYGLFINV
                     WIAFFNLIPFGPLDGAKIIRWNPLVWGILIAIPLVFLFASGIF"
     gene            complement(1896..2693)
                     /locus_tag="PLK55_04085"
     CDS             complement(1896..2693)
                     /locus_tag="PLK55_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOZ36133.1"
                     /translation="MEIEINYELSAGKNAENKYKGRNRYLEKIKGIDKALEITKKKMT
                     LLEKEKANKPKLEISKKEERNKEWFEKFKWFYSTNGFLVLAGRDAKNNEQLVKKHFAI
                     TDLYFHADIHGAPHTLLLNPDKREIKEIDKEEAANFAAIHSSAWGSGTYTVDVYSVGY
                     GQVTKTAGSGESLGTGAFVIRGKRDYYKKIDLKIGVGFDSKRKVVIAGPVSAISFVSK
                     DIFTLVPGKTKKSDICKELKEKFSKKGIAVSVDDLMSNLPPGDCEIK"
     gene            complement(2695..3234)
                     /locus_tag="PLK55_04090"
     CDS             complement(2695..3234)
                     /locus_tag="PLK55_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOZ36134.1"
                     /translation="MDDSKKILDPIRIKILSAMRKQGVLVPNIRQLKKHTGFHRATIK
                     SSIDLMEKQGLIKQYIPSLNSSVAGYGLRVWTFVQMDISNEKLTNNLKKIVTTIPNIY
                     HCSEVITEKGYNIAIGYLAKNVEDYYNNIQRKYFLNYPEIYNNIKERTVFYLSEPTYV
                     QKSHSSALIDILEREQGLE"
     gene            complement(3302..4516)
                     /locus_tag="PLK55_04095"
     CDS             complement(3302..4516)
                     /locus_tag="PLK55_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ36135.1"
                     /translation="MAENIFYNFLNENSVFTDKSKISPHYAPEKLPFRDHQIKDLSLW
                     LSPVLKKAKPHNVFVYGKVGTGKTTVVRHVISQLELFSKDQHLPLVTVYLNCRNYQTK
                     YTVLVYCVQLFYPDQNFFGYSRSFIYDKFLTYIKENKINFVVVLDEIDKIKDKEIDDL
                     IYTLTRANDELEGGSVSIIGISNNLFFKNNLDSRTKSSLCEQEAVYPPYNAEELRDIL
                     TQRIDIAFKKEVVSASAINAAAAIAAKESGDARRAVMLLLRAGEISDLNGNTKVTDKE
                     VYEAKEKVEQEVMKSMVLTLPVQLQLVLLAIAQGSLKPKGILGVTGQSMDSNILYSGE
                     IYDKYCTLCEKYSEKAVTIRWFREYITELETYGFISTTISGKGVKGNTTLIRLGIDAQ
                     TIIDLLKQEFSE"
BASE COUNT         1499 a          787 c          667 g         1662 t
ORIGIN      
        1 ccattaactg ccaggtcgta gccgtttatc tcatcatcgt cttcgcttaa tatctcttct
       61 gatggcacat acggggggtt aaagattatt aaatcaaatt tctttttctc atctatagaa
      121 tcaaaaagat tagaataggt tattttttgt ttctctagat tatattgttc tttgacattc
      181 aaaagataat caataactat tggattaata tctgtagata ttatctcttt aacattatca
      241 taaatctcta aacttaaaaa accgttacct gaacccagtt ctattgccga atcaaatgtt
      301 ttgtttgatt ttattatttc tgcatctaaa acctctatca ttagatcagt atcgtcgctt
      361 ggcaaataca ctcttgcagg aatatctaaa ctatattgtt taaaattaag tatagtcata
      421 tattatatat ggagaataaa gatttaaaat tcatagacat tcactgccac tatgatgaat
      481 tatctttaga atgcctaaaa gaaacattct ctaaagaaga aatgattgca atcgcaaaca
      541 acgtagacat agagagctac agaaaacttg aacgcctaag aaaagaaaac atccccgggc
      601 tttactttgc atatgggtta tatcctgatg ttgttgttaa agttggacta aaggattccc
      661 ttaattatct taaaataata tttgaaaact ccaaaaatat tcttgcaatt ggagagattg
      721 gcctagatta taaaataact aaagatttag aaattagaaa agaacaaaaa attgtttttg
      781 aaaaacagct agagattgct aaagaaagaa acttgccagt aatcatacac tctagatatg
      841 caacaaaggc agttcttgat gtgctttcag gatattctgg atttccaata atccttcact
      901 ggtttagtgg aaatgaaaaa gaaatacaaa cagcacttga tagggactat tatctaacgc
      961 ttagatttaa taaaccaagc atacctaata ttaaagaaca tttaaatcat atttttatag
     1021 aaacagatta tccaatctct tatgatggaa aacctcttga aatcacagac ataaaaaaat
     1081 cctatgaaat atttgcaaaa gaaaataata ttgatttaga atatcttaaa gaaaaaataa
     1141 ataataattt taataaagtt tttttaagaa gataaaaggg aaaatatatg ataaaaaaga
     1201 actctgaaat ttatgaccta ttaattgcat ggcttgcaat atctctatgt tttacaatag
     1261 ttcttggtaa ctatagcctt ttagattatc ttaccacaaa acaatttaat tttacttggg
     1321 cagatttctt tacaaaattt ggattgagcc taataatcac ttccacgtct tttatattcc
     1381 acgagcttgc acacaaatat atgggaatac accttggggc acacggcaga tttattatgt
     1441 ggccggtgtt tttaatcgca ggtgttgctc ttgcaggatt acttggagtt gtttttgtgg
     1501 ctcctggagc agtttatatt tttggtaaac aacataatat taaagaagac ggaataattt
     1561 ctcttgctgg ccctgcaaca aatctaatta tggcgttact attttttatt gcagcgttat
     1621 tcggggcccc aattttaatt gttagttacg gattattcat taacgtctgg attgcgttct
     1681 ttaatttgat accttttgga ccacttgatg gcgcaaaaat aataagatgg aatccactag
     1741 tctgggggat attaattgct atacctttag tgttcttatt tgcgtctggt attttctaat
     1801 tttctttttt taattttatt aacttttaga attggcctgg atgttagtct tctaaaatta
     1861 gtttttggag tcgctcccat agttatttac ctcttttatt ttatttcaca atcgcctgga
     1921 ggaagattag acatcaaatc atcaacagaa acagcaatac cttttttcga aaatttttct
     1981 tttagttctt tacatatatc tgatttcttt gtttttcctg gaactaaagt aaagatatct
     2041 ttacttacaa aagatattgc agagactggc ccagcaatta caacttttct tttgctatca
     2101 aagccaacac caatctttaa atcaattttt ttataatagt ctcttttgcc tctaataaca
     2161 aatgcgccgg ttccaagaga ctcaccacta cctgcagttt tagtaacctg gccataacca
     2221 acagaataga cgtcaacagt ataggtgcca ctgccccaag cagaagaatg gattgctgca
     2281 aaatttgcag cttcttcttt gtctatttct ttaatttctc ttttatctgg atttaataat
     2341 aaggtatgtg gcgcgccatg aatgtctgcg tgaaaataaa gatcagtaat tgcaaaatgc
     2401 ttttttacaa gctgctcgtt gttctttgca tctcttcctg ccaatactag aaaaccattc
     2461 gtgctgtaaa accacttaaa cttttcaaac cactctttgt ttctttcttc ttttttagaa
     2521 atttcaagct ttggtttatt tgctttttct ttttcaagta aagtcatttt ctttttggtt
     2581 atctctaatg ctttgtctat tcctttgatc ttttctaaat aacgatttct gcctttatat
     2641 ttgttttctg cattcttccc tgcagataac tcgtagttta tttctatttc cattttattc
     2701 tagtccttgc tctctttcta aaatatcaat taatgcacta ctgtggcttt tctgaacata
     2761 ggttggttca gacaaataaa atactgttct ttcttttata ttattataga tttctggata
     2821 atttaaaaaa tactttcttt gaatattatt ataataatcc tcgacattct ttgccaaata
     2881 accaatcgca atattatagc ctttttctgt tattacttca gaacaatgat aaatatttgg
     2941 aattgtagtt actatttttt taagattatt tgtaagcttt tcattagata tatccatttg
     3001 aacaaaagtc caaaccctta agccataacc tgcaacacta gaattaagcg aaggaatata
     3061 ttgtttgatt aatccttgct tttccattaa atcaattgaa gacttaattg ttgctctgtg
     3121 aaaacctgtg tgcttcttta attgtctaat attaggtact aacacgcctt gctttctcat
     3181 agctgacaaa atcttaattc ttattgggtc tagaatcttt ttagaatcat ccataactag
     3241 tcacaaataa tatatgcaaa aaaagatata taaatggaag taattgttcc aattggcaca
     3301 actattccga aaactcttgc tttaaaagat caataattgt ttgcgcatca attcctagtc
     3361 ttattaacgt cgtattacct ttaacgcctt taccagagat tgttgtagaa ataaaaccat
     3421 aggtttcaag ttcagtaata tattctctaa accatcttat ggttacagct ttttcagaat
     3481 atttctcgca aagagtacaa tacttgtcat agatttctcc agaatataaa atatttgaat
     3541 ccatggattg accagttacg cctaagatgc ctttaggctt aagagaacct tgagcaattg
     3601 caagaagtac taactgtaac tgaacaggaa gagttaagac catgcttttc ataacctctt
     3661 gctcaacttt ttctttagct tcgtagacct ctttgtctgt gacttttgtg ttaccattta
     3721 gatcagaaat ctctcctgct ctaagaagta acatgactgc acgtctggca tctcctgact
     3781 cctttgcagc aattgccgct gctgcattta ttgctgaagc gctgacaacc tcttttttaa
     3841 aagcaatatc tattctttga gttaagatat cccttaattc ttcagcatta taaggcggat
     3901 aaactgcctc ttgctcacag agagaacttt ttgttcttga gtcaaggtta ttcttgaaaa
     3961 aaagattgtt tgaaatacca ataattgaaa cagacccccc ttcaagctcg tcgtttgctc
     4021 ttgttaaagt ataaataaga tcatcaatct ctttatcctt gattttatca atctcgtcaa
     4081 ggaccacaac aaaattaatt ttattctctt taatgtatgt taaaaactta tcatatataa
     4141 aagacctaga ataaccaaag aaattttggt caggataaaa taattgaacg cagtatacta
     4201 gaacagtata ttttgtttga taatttctac aatttagata aaccgttact aagggtagat
     4261 gctgatcttt ggaaaaaagt tcaagctgag aaataacgtg tcttacaaca gttgtttttc
     4321 ctgttccaac tttaccataa acaaaaacat tatgtggctt tgcttttttt agaacaggag
     4381 aaagccataa agacaaatcc tttatctgat ggtctctaaa aggcaatttt tcaggagcat
     4441 aatgcggaga aattttagac ttatcagtaa aaaccgaatt ttcatttaaa aaattataga
     4501 atatattctc tgccatcttt taccacgctc taaactaaaa agttaaatct attaccagaa
     4561 cttattccat agagaatact atatattaag aatcaaaggc tttttaagtg tgtgg
//
LOCUS       DAOOON010000042         4205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_25040_length_4205_cov_23.801205, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000042 DAOOON010000000
VERSION     DAOOON010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25040_length_4205_cov_23.801205"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..652)
                     /locus_tag="PLK55_04100"
     CDS             complement(182..652)
                     /locus_tag="PLK55_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36136.1"
                     /translation="MLKSKIYKKGVSPLIATILLVAVSLSLAGILYSWASQNAGNTVS
                     SVTETQQQWTNCTAINLFIEEGCNYDTNTGISLILSDHSSVDIDENLEISVTDTNNLI
                     KATSFAPNFQGGAMAINSSVFSDPDALDGLGQIQRVYVFVKSCPDRKATTMSCD"
     gene            complement(699..1511)
                     /locus_tag="PLK55_04105"
     CDS             complement(699..1511)
                     /locus_tag="PLK55_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ36137.1"
                     /translation="MKFSPRIEYLLDAAAKGAIGSLILGVFLLRTEFFIFCIFPVIIA
                     IGIASIVYTKKIRSLEANLFVFLDDLKDLLQGGMNIVTSMEVLSEHDYGALNVYIRRI
                     TAQVKIGVPFETSLVEVFAEIDSPMFNKIANVIAETMKYGGNLIKIFSAVSNYVKLID
                     EMGEERRSKTFSTVFSSYFMFFIFIAIILTIQIVFLPMLKSNDINTSGASIEDIPFNA
                     YFLYLLIIQASFAGPIIGKISEGNAMAGIKHSIILLVISVPIYVIVSMVFVK"
     gene            complement(1522..2409)
                     /locus_tag="PLK55_04110"
     CDS             complement(1522..2409)
                     /locus_tag="PLK55_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOZ36138.1"
                     /translation="MSATKDKVVKSNTSLEESLYKLFLPLAHQLKSKELDEKLMQADI
                     LEEQEVYLSRYLFYAICIPVVSVILFSALLFAFNYTEYLKILFIVGFSLVFIILIYAM
                     VNPYLKVGTKAGDIKNNLPLAILSMSSVAESGAPPEAMFHTTSVKSETPHISREFDKI
                     NRYLDLGLSLPEAMDQLCAKTPSPELKKFLMELKSNIEAGGSLPEFMKKKAEHAQFTY
                     KLMLDDLNKKAETFGDIYSAIVIAGPLFLFSSIMLLGMIGGGGGLAGLSIEALLLIGV
                     FGLVPLVNIFFIIILQFMS"
     gene            complement(2520..3626)
                     /locus_tag="PLK55_04115"
     CDS             complement(2520..3626)
                     /locus_tag="PLK55_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOZ36139.1"
                     /translation="MPANASYEFGVAQRKYDEAKTDEEKVIALQGMLSTAPTHKGSEN
                     LRRDISKRIADLKNKMEKRAAALKKTGHSIGIRKEGAGQVVIIGLANSGKSTFLTKFT
                     NAKPVIAPYPFTTTKPEIGTLNYGGALIQLVELPSFLESKEMANKLFSIVRSADAIVF
                     LVPNNSLEQRDKLIYLLESQDIYVTKKKPKIELNKSSELGITFINEQNLAMPKEQAIS
                     LLKDSGYRNHTVILGQKTNTNDLLLLMNPRAVYIPSIFVIMPFSKVKADIDNYKNIKF
                     FDFKDETKITEEIFKMVNKIIVYTKKPGQKADLSDPLVLEKGTTVKIAAKQIHKSISK
                     ELKSAKVWGSTRFPGQNVSKDYVLQDRDIVEFVA"
     gene            complement(3630..>4205)
                     /locus_tag="PLK55_04120"
     CDS             complement(3630..>4205)
                     /locus_tag="PLK55_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36140.1"
                     /translation="WVEDMLKERKEKWISVGNQIFPKVEKSIRQDLVYLKNKKMPEIF
                     VLGPGEGAEVVFINELLQKNLPKIDTLSITNQLSKEANKIIRKDYSSNPKTLSKKDLF
                     ENFNHLKFVKKYDYIFTNMGPLEHTRFQEIAILKLASMLRPGGLARVIPAISLDCIKN
                     IKEYLKQKGKENDLSFENSVYDGQLLIKRIK"
BASE COUNT         1386 a          723 c          566 g         1530 t
ORIGIN      
        1 tgaattgttg ctttatcaca tttttgatta accaattcat ttgctgtatt tacaccagtt
       61 gttgtaaagt ttctacccca agccaataga atcataatta aagaaacaga aataactact
      121 aacaaaacag tagtaaccaa aacattaagt gcttttaatt ttttcataaa taaaatctaa
      181 tttaatcaca actcatggtt gtcgcttttc tgtcaggaca gcttttaaca aaaacataaa
      241 ctctttgtat ttgacctaag ccatcaagcg cgtcaggatc agaaaatacg ctgctattaa
      301 ttgccattgc ccctccttga aaatttggag caaagcttgt ggcttttatt aagttgtttg
      361 tgtctgtaac actaatttct aggttttcgt ctatatcaac actagaatga tcacttaaaa
      421 taagactaat accagtattt gtatcatagt tacaaccttc ttctatgaac aaattaatgg
      481 ctgtacagtt tgtccattgc tgttgagttt ctgtaacgct tgaaactgta ttacctgcat
      541 tttgagaagc ccaagaatat aagattcctg caagagataa agatactgca actaatagaa
      601 ttgtagctat aagaggagaa actccttttt tataaatttt tgattttaac atattttatc
      661 ctattaatat atatttcaca tatttatata aatctaactt atttaacaaa caccattgaa
      721 acaatcacat agattggcac agatattact aataatatta tagaatgctt aataccagcc
      781 attgcgttac cctcagaaat ctttccaatt attgggccag caaaacttgc ttgaattatc
      841 aaaagataca aaaaatatgc attaaatgga atgtcctcta ttgatgctcc agacgtattt
      901 atatcgtttg attttaacat tggcaaaaat acaatctgta ttgttaatat aattgcaata
      961 aaaataaaaa acataaaata agaagaaaaa actgtagaaa atgttttaga tcttctttct
     1021 tctcccatct catcaattaa ttttacatag tttgatacag cactaaatat ctttataaga
     1081 ttgccaccgt acttcatagt ctctgcaata acatttgcaa tcttattaaa cattggactg
     1141 tctatttctg caaaaacttc aaccaacgaa gtttcaaaag gcacacctat cttaacttgt
     1201 gcagttattc ttctaatgta gacatttaat gcaccatagt cgtgttctga aagaacctcc
     1261 atagaagtta ctatgttcat tccgccttga agaagatctt taagatcatc tagaaaaaca
     1321 aataaattgg cttcaagaga ccttattttt ttagtataaa caatagaagc aatgcctatt
     1381 gcaattatta ctgggaaaat acagaagata aaaaattctg tcctgagtaa aaatactcca
     1441 agtataagtg acccgattgc tccttttgct gcagcatcaa gcaaatactc tattcttgga
     1501 ctgaatttca tttttttcct cttaagacat aaactgtaaa ataattataa agaaaatatt
     1561 aactaaaggc actaggccaa acacacctat caataataac gcctcaatac ttagaccagc
     1621 tagaccacca ccaccgccaa tcatacctag aagcataatt gacgaaaata aaaataatgg
     1681 ccctgcaatt acaattgcag aataaatgtc gccaaaggtt tctgcttttt tattaagatc
     1741 atcaagcatt aacttataag taaactgcgc atgctctgct ttttttttca taaattctgg
     1801 tagagaaccg cctgcttcga tattagattt taattccatt aaaaatttct ttaactcggg
     1861 agaaggagtt tttgcacaca gttgatccat tgcttcaggt aaagacaacc ctaaatctaa
     1921 atatctattt attttatcaa actctctact tatgtgagga gtctcagact ttactgaagt
     1981 ggtatgaaac attgcttctg gcggcgcacc agactctgca acactagaca tactaagtat
     2041 tgctaaaggc aaattatttt ttatgtctcc agcctttgtc ccaactttaa gatacggatt
     2101 aaccatcgca taaatcagta ttataaaaac caatgaaaat cccacgataa ataaaatctt
     2161 aagatattct gtataattaa aggcaaataa taacgcagaa aaaagaatta cagaaacgac
     2221 aggaatacag atcgcataaa aaagatatct ggagagataa acttcttgtt cttcaagtat
     2281 atctgcttgc attaattttt catcaagctc tttagatttt aattgatgag caagaggcaa
     2341 aaataactta tataagcttt cttctaaact tgtatttgac tttacaacct tgtcttttgt
     2401 agcactcatt tttgattttc taattataaa tataattatc cttaataaaa tatatttttc
     2461 taaaataata tatatgtatt tcttttataa agctttttct ttaagaaaaa atactaagat
     2521 tatgctacaa actcaacaat gtctctatct tgaagcacat aatccttaga aacattctgc
     2581 ccaggaaatc ttgtactgcc ccaaactttt gcagatttta gttctttaga aatagatttg
     2641 tgtatttgtt ttgcagcaat cttaacagtt gtgccttttt caagaactaa tggatctgat
     2701 aaatctgctt tttgccctgg cttttttgta tagactatta ttttatttac catcttaaaa
     2761 atttcttctg taatttttgt ttcgtctttg aaatcaaaaa attttatgtt tttatagtta
     2821 tcaatatctg cttttacttt agaaaaaggc ataattacaa aaattgaagg aatataaact
     2881 gctcttggat tcattaacaa taataagtca tttgtatttg ttttttgtcc aagaataacg
     2941 gtgtgatttc tgtagcctga gtcttttaaa agagagattg cttgttcttt tggcatcgct
     3001 aaattttgct cgttaataaa ggttatacca agttcagaac ttttgtttaa ttctattttt
     3061 ggttttttct ttgtaacata gatgtcttga gactctaaaa gataaataag tttatctctt
     3121 tgctcaagag aattatttgg aactaaaaaa acaatggcat cggcagatct tacaatagaa
     3181 aataatttat ttgccatttc ttttgattcc agaaaagatg gaagctcaac taattgaatt
     3241 aatgcgccac cataatttaa tgtgccaatc tctggttttg ttgttgtaaa ggggtatggc
     3301 gcaattacag gtttagcatt tgtaaatttt gttaaaaagg tacttttacc agaattcgct
     3361 aagcctataa ttactacttg accagcgcct tcttttcgta ttcctattga atggcctgtt
     3421 tttttaagag ctgctgctct tttttccatt ttatttttta agtctgctat tcttttactt
     3481 atgtctctgc gcagattttc agaaccctta tgagttggcg cagtcgataa cataccttga
     3541 agagctatta ctttctcttc gtctgttttt gcttcatcat attttctttg agcaacacca
     3601 aattcataac ttgcatttgc tggcataatt tattttatcc ttttaattaa taactgacca
     3661 tcataaacac tattttcaaa agacaaatca ttttccttgc ctttttgttt taagtattcc
     3721 ttaatattct ttatacagtc taaagatatt gcaggaatta ctcttgcaag gccaccgggc
     3781 cttaacatgc ttgcgagttt taaaattgca atttcttgaa atcgagtatg ttctaggggg
     3841 cccatattag taaatatgta atcatatttt ttaacaaatt ttaaatgatt aaaattctca
     3901 aataaatctt tttttgaaag cgtttttggg ttggatgagt aatcttttct tataatcttg
     3961 tttgcctctt ttgacaattg attagtaata cttaaagtat caatttttgg taaatttttc
     4021 tgtaataatt catttataaa aaccacttct gcgccttccc ctggccctaa cacaaaaatt
     4081 tccggcattt ttttattttt taaataaact aggtcttgcc ttatagattt ttctactttc
     4141 gggaatattt gatttcccac agaaatccat ttttcttttc tttctttaag catatcctct
     4201 accca
//
LOCUS       DAOOON010000043         4200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_25076_length_4200_cov_10.786248, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000043 DAOOON010000000
VERSION     DAOOON010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25076_length_4200_cov_10.786248"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1194
                     /locus_tag="PLK55_04125"
     CDS             115..1194
                     /locus_tag="PLK55_04125"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006330.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOZ36141.1"
                     /translation="MTLTIEERLELIKEVGEEIIGEDELIELLKTNKSIIAYDGFEPS
                     GKIHIAQGLMRAINVNKLTKAGIHFKFWVADWFALMNNKMDGDIEKIRTVGEYFIEVW
                     KASGMDMKGVEFLWASENMNQEYWLMVLKIARINTVARVTRCSQIMGRSEKDELSAAQ
                     IFYPCMQAADIWNLKADVAQLGMDQRKVNMLARETFPKLGLKKPVAIHHHMLMGLTEP
                     KTEGLEGAEAAVAKKMSKSNPDSAIFMTDTKEEIDRKFKKAYCPEGIVKDNPVLEYCK
                     YIIFEKIKEFKIERPEKFGGNVVYKSYQDLEKDFVDKKLHPMDLKTASAKYINELIEP
                     VRNHFQTDSKAKALLAKVESYIVIK"
     gene            1206..2897
                     /gene="gltX"
                     /locus_tag="PLK55_04130"
     CDS             1206..2897
                     /gene="gltX"
                     /locus_tag="PLK55_04130"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOZ36142.1"
                     /translation="MSNNDSIKQLVLENAVKYKGKADKKFVFGAILANYPEYRTKQQD
                     LHEILDRFIAEINSLDLDKQKQELEKLNPEFFEKKEKEERNIFAFLNIKQGDKVISAF
                     PPSPEKYYHIGHAKAILLNYLLAEKYKGKFYLRFEDTNPENVLPEFYDIMLEDIGWLG
                     VKWDGVQYASDYMDLFISYAERLIKEDKAYMCFCPAEVASEKRNKKEECACRNHTVDE
                     NLKFWKELPSYSEGKAVLRLKIDMSHKNTRMRDPTIFRRIDKPHPRIGSKYNIYPMYD
                     FQNPVMDGYFNITHRFRTVEFEMASELHHYIRKILGLYDTYTYEFSRFNLEGMESSGR
                     VIREKVNSGELIGWDDPSLPTLRALKRRGFTPEAIKQLVVESGITKASGSLLTWDTLI
                     KYNKRILNDTSKRFFFIKEPVELEVQGDLKKDFSLEMHPSLSLGKRDFKSNGSYFLEK
                     EDLESLKDKTLVRLMDNINFIYDAKTKTSKFYSEDYLSFKNFSGEKKIIHYLPKDDTQ
                     IIKASVFFPDHKLIKGPAEKNIDTLKIGEIIQFERFGFCRLDSIKDNVYNFWFAH"
     gene            2912..3475
                     /locus_tag="PLK55_04135"
     CDS             2912..3475
                     /locus_tag="PLK55_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOZ36143.1"
                     /translation="MLQKINDEVYKIACDSNMYYIKPLSLVIDTGNFQERAMVERELT
                     SIVDPSTIKTVIFTHLHYDHIGNFNMFPKAKFYAHKEEITYFEQNKEGAVLNPVIVKK
                     FTAKLISIARLKLPKRYQIIHTPGHTCGSICILDTSKKILFSGDTLFYDGHGRVDLPS
                     SEAKKMEKTLDLIGELDFEILCPGHDY"
     gene            3488..4045
                     /locus_tag="PLK55_04140"
     CDS             3488..4045
                     /locus_tag="PLK55_04140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOZ36144.1"
                     /translation="MKQENHSYETIVLNNQNEVMDFYKKNPSKFRQIYDLFDKELNFG
                     TGSKTKFPVWLKELAAGPKTTFFLITNNKEIIGAIYGYDVVLNKHFELSMIAVKKEYQ
                     GKLYGTKLMTKAMSIIFRKGYEKIEMLALEKEKKLLEYLTGNLVDKLGKKRKFNSDFE
                     YKLNKDLVGTHRITKVSKPRRLIAK"
BASE COUNT         1625 a          548 c          740 g         1287 t
ORIGIN      
        1 tcttttactc cattggcatt aggtgttttt gttttaaaat taatatttgc attagttata
       61 atttatttaa taaaattatt ttttgaaaaa tacagaatat aaagaaagat atatatgact
      121 ttaacaattg aagaacgatt agaattaatt aaagaagttg gagaagaaat tattggagaa
      181 gacgaattaa ttgagcttct aaaaaccaat aaatcaatta ttgcatatga tggttttgag
      241 ccatctggga aaatacatat tgctcaagga ttaatgaggg caataaatgt aaataaacta
      301 acaaaagcag gaattcattt taaattttgg gtagcagatt ggtttgcatt aatgaataat
      361 aaaatggatg gagatattga aaagattaga acagttggag aatactttat agaagtttgg
      421 aaggcttctg gaatggatat gaaaggcgta gaatttttat gggcttctga aaatatgaat
      481 caagagtatt ggttaatggt attaaagatt gcaagaataa atactgttgc aagagtaaca
      541 agatgctcgc agattatggg aagaagcgaa aaagatgagc tttcagcagc acaaatattt
      601 tatccatgta tgcaggccgc agatatctgg aatcttaaag cagatgtcgc tcagcttgga
      661 atggatcaaa gaaaagtaaa tatgcttgca agagaaactt tccccaaact tggtttaaaa
      721 aagccagtag caatacatca ccatatgctt atgggtttaa ctgaaccaaa gacagaagga
      781 cttgaaggtg cagaagcagc ggttgcaaag aagatgtcaa aatcaaatcc agattctgca
      841 atctttatga cagacactaa agaagaaata gatagaaaat tcaagaaggc atattgtcca
      901 gaagggatag ttaaagataa tccggtatta gagtattgta aatatataat atttgaaaaa
      961 ataaaagaat ttaaaattga aagaccagaa aagtttggag gaaatgttgt ttacaaaagt
     1021 taccaagatc ttgaaaaaga ctttgtagac aaaaagcttc atccaatgga tctaaagaca
     1081 gcaagtgcta aatacattaa tgaattaata gaaccagtaa gaaatcattt tcaaacagat
     1141 tcaaaagcaa aagcattact tgcaaaagtt gaaagttata ttgtaataaa ataattaaga
     1201 taaatatgtc aaacaacgat tcaattaaac agttagtttt agagaatgct gttaaatata
     1261 agggaaaagc agataagaag tttgtttttg gagcaatcct agcaaactat cctgaatatc
     1321 gaacaaaaca acaagatttg cacgaaatct tggacaggtt tatagccgaa ataaactctt
     1381 tagatttaga taaacaaaaa caagagcttg aaaagcttaa tccggagttc tttgaaaaaa
     1441 aagaaaagga agaaaggaat atttttgctt tcctaaatat taaacaagga gacaaagtaa
     1501 taagtgcttt tcctccaagt ccagagaagt attatcacat aggtcatgct aaagcaattc
     1561 tattaaatta tctcttagcc gaaaaataca aaggtaaatt ctacttaaga tttgaagaca
     1621 caaatccaga gaatgttctt cctgagtttt acgatatcat gcttgaagac attggttggc
     1681 ttggtgttaa gtgggatggt gttcaatatg cttctgatta tatggacctt tttatttctt
     1741 atgctgagag gcttataaaa gaagacaaag cctacatgtg cttttgtccc gcagaagttg
     1801 caagcgagaa aagaaataaa aaagaagaat gtgcttgtag aaatcacaca gttgatgaaa
     1861 atttaaaatt ctggaaagaa ctaccaagct attcagaggg caaagcagtt cttcgcctaa
     1921 agatagatat gtctcacaag aacacaagaa tgagagatcc aaccattttt agaagaatag
     1981 ataaacctca tccaaggatt ggaagcaaat ataatattta tccaatgtat gattttcaaa
     2041 atccagtaat ggatggttat ttcaatatta ctcacaggtt tagaactgtt gagtttgaga
     2101 tggctagtga gcttcatcat tatattagaa aaattttagg tctttatgac acatatactt
     2161 acgagttttc aagatttaat cttgaaggca tggaatcaag tggtagagtg attagagaaa
     2221 aagttaattc tggggagttg attggttggg atgatccttc tcttccaact cttagagccc
     2281 taaagagaag gggctttaca cctgaagcaa ttaagcaatt agtagttgag agtggaataa
     2341 caaaagcgtc tggttcatta ttaacctggg atactttaat caaatacaat aaaagaatat
     2401 taaatgatac ttccaaaaga ttcttcttta ttaaagagcc tgtagagctt gaggtacaag
     2461 gagatttaaa gaaggatttt tctttagaaa tgcatccaag tctttctctt ggcaaaagag
     2521 atttcaaatc taatggcagt tatttcttag aaaaagaaga cctagagtct ttaaaagaca
     2581 aaacccttgt aagactaatg gacaatatta atttcatcta tgatgcaaaa acaaagacta
     2641 gtaaatttta ttcagaagat tatttgtctt ttaagaactt ttctggggaa aaaaagataa
     2701 tacattatct tccaaaagat gacactcaaa taattaaagc aagcgttttt tttccagatc
     2761 ataagcttat aaaaggccca gcagagaaaa atattgatac tttaaagatt ggagagatta
     2821 ttcaatttga aaggtttggc ttttgcagat tagattctat aaaagataat gtttataatt
     2881 tttggttcgc acactaaagg agaaaaataa aatgttacaa aaaataaatg atgaagtata
     2941 taagatcgca tgtgattcta acatgtatta cataaaacca cttagtctag taatagacac
     3001 aggcaacttt caagaaagag caatggttga aagagaactt acaagtattg ttgatcctag
     3061 cacaataaaa acagtaattt ttactcatct ccattatgac catattggta actttaatat
     3121 gtttccaaaa gctaaattct atgcccacaa agaagaaatt acttattttg aacaaaacaa
     3181 agaaggtgca gttcttaacc cagtgattgt taaaaaattt actgcaaaat taattagtat
     3241 agcaaggctt aagcttccaa aaagatatca aataattcac actccagggc acacctgtgg
     3301 ttcaatctgt attcttgata cttccaaaaa gatacttttt tcaggagaca ctcttttcta
     3361 tgatggtcat ggtagagttg accttccaag ctcagaagca aagaaaatgg aaaagacttt
     3421 agatttaata ggagaattag attttgagat tctttgtcca ggtcatgatt attaattctg
     3481 aaagattatg aaacaagaga atcactctta tgaaacaatt gttttaaata atcagaatga
     3541 agtaatggat ttctataaga aaaatccctc taagtttaga cagatctatg atctttttga
     3601 taaagaatta aattttggta ccggttcaaa aaccaaattt ccagtttggt taaaagagtt
     3661 agctgctggt ccaaagacta cttttttttt aattacaaat aataaagaaa taattggcgc
     3721 tatctatggt tatgatgttg tcttaaataa gcattttgag ttatctatga ttgctgttaa
     3781 aaaagaatat caaggaaagc tttatggtac aaaacttatg actaaagcta tgtcaattat
     3841 ttttagaaaa ggctatgaaa aaatagagat gctagcttta gaaaaagaaa agaaattatt
     3901 agaatatctt acaggtaatt tagtagataa attaggtaaa aaaagaaaat ttaattctga
     3961 ttttgaatac aagttaaata aagatttagt tggcactcac agaataacaa aagtttcaaa
     4021 acccagaaga ttaattgcaa aatagttatt ctacagtaat tttaaatttc tttagttctt
     4081 tttttagttc ttcatagtct cctttgtaat gcaaagcgtt catccctagt tttcttggtg
     4141 gcaacacatc atcaataagc ttgtctccaa tcattattgc ttcttctggc ttacatttag
//
LOCUS       DAOOON010000044         3913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_27600_length_3913_cov_15.378694, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000044 DAOOON010000000
VERSION     DAOOON010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27600_length_3913_cov_15.378694"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLK55_04145"
     CDS             <1..500
                     /locus_tag="PLK55_04145"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOZ36145.1"
                     /translation="LFLGTIIFLLLNFNTTYYYFAYGSNMDIDLLRNRIDNNYLFSEG
                     YAKLENYKLVFPRGVGSVVSDETTDVYGCLYLLNKKEIEKLDVVEGYRKDRNKSENSY
                     NREWIDVVLQNGKTLSAEIYIQTNNTDINGKPSNSYKQTLVRGASDCSLPDFYIQELE
                     AIETK"
     gene            639..1447
                     /locus_tag="PLK55_04150"
                     /pseudo
     CDS             639..1447
                     /locus_tag="PLK55_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(1522..2112)
                     /locus_tag="PLK55_04155"
     CDS             complement(1522..2112)
                     /locus_tag="PLK55_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36146.1"
                     /translation="MHPTRLKLERQFKTAKNIKPKKSTKITFDNHVLYVKEKNALNLE
                     LESILSKRKNPNLYLPIIINGLIDLARKNGYSTITGKTWIFAEHPRIAERMGISVDSK
                     ALEAYNALKKEYNILKIQGISKKIILSKSSFLLEKNIKFQKMVKTIDENSFSRMARNH
                     EPYIIVNTIIKTEKGLKNKTFLLPKKLILFPFEIKL"
     gene            complement(2123..2680)
                     /locus_tag="PLK55_04160"
     CDS             complement(2123..2680)
                     /locus_tag="PLK55_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOZ36147.1"
                     /translation="MEYCLFCLHKKEIDKYRRDLVNQIYKQFGLKHTKKANNYAHFTL
                     KYKFKANSKQLKELEKTLLKFSKIHNKEKIKVGGFDNFHKRVVFVKVSQSPKVKKLIF
                     DLHKELRKLKWLTWHNNIEGDGIHLHTTITEESNEKLYPKIMKYLSGKEKYFDSYFDN
                     VTIMKYSTAKKPKRRCVIAKRYNLQ"
     gene            complement(2694..>3913)
                     /gene="aspS"
                     /locus_tag="PLK55_04165"
     CDS             complement(2694..>3913)
                     /gene="aspS"
                     /locus_tag="PLK55_04165"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOZ36148.1"
                     /translation="MFFILRDQYKKEQITFFKKDNPEMFEKAKAITKESVIIVTGTKK
                     DSEQAMNGYEVIPETLEILSISDAPVPLDISGKIDSGFDARINYRYIDLRVPENLAIF
                     KVRSKIVRAVTDFFDEKGFININTPKMVSNGVESGSELFEVKYFDRKAFLSQSPQVYK
                     QMMIASGFNKVYEIGEVFRAEKSHTTRHLTEFTGIDLEMGYIKDPEEVYNVLEELMAY
                     VLTEVKKECKTELELLKVEIKIPGKIPRIKMKDAKALLKAKGKEIPAEEDLDSEGEKM
                     LGDIVKEKFNSEFVFLTEFPFSKRPFYHMCEIDPETKQKITKSADLIWKGSEICTGAQ
                     REHRTSVLKEQITEKGMSLEDMDYYVKMLSYGVPSHGGAGLGLDRITQLMLNLANVKE
                     AVLLPRDPERLTP"
BASE COUNT         1277 a          607 c          585 g         1444 t
ORIGIN      
        1 ttcttttctt aggaaccatt atttttttat tattaaactt taacacaact tattattatt
       61 ttgcatatgg aagcaatatg gatattgatc ttctaagaaa cagaatagat aataactact
      121 tgttttcaga aggctatgca aagcttgaga attataaact cgtttttcca agaggtgttg
      181 gttctgtagt atctgatgaa acaacagacg tctatggctg cctttatctt ttaaacaaaa
      241 aagaaataga aaagcttgat gttgtagaag gctatagaaa agacagaaac aaatcagaaa
      301 acagttacaa tagagaatgg atagatgttg ttttacagaa tggtaaaacg ctttctgcag
      361 aaatatatat tcagacaaac aacacagata ttaatggcaa gccaagcaat agctataagc
      421 aaactttagt aagaggcgca agcgactgtt ctcttcctga tttttatata caagaattag
      481 aagccataga aactaaataa gttttaattt ttgtaaattt tcaaattaaa tataataatt
      541 ttgtatttat acaaataaaa caaacagatt tcgcactgga cagctacccc cccgagaaca
      601 cccccgtttt caaaaaccat attattttgg gaataattat gaataaagta aatgtgttag
      661 gcactgatat ttcgtattat acaaaaaagg aaaatgatta tatatcttta acagacatgg
      721 ttaaaaattt aaataatggt ttagtattaa tagaaaagtg gttaagaaat aaaatactat
      781 tgaatttata ggtgtttggg aagagatgaa taatccagat tttaattccc ccgaattcgg
      841 gggaattaga aatgaagccg gaacaaatag atttgcgttg tctgtaagtc aatggataat
      901 tagaacaaac gcaattggga ttagggctag tgcagggagg tatggcggaa cctttgcaca
      961 taaagatatt gcttttgaat ttgcctcgtg gatatctcct aaatttaaat tatatctaat
     1021 taaggaattt caaagattaa aaatagatga aatccaaaga cttaaacttg attgggatat
     1081 taaaagaaat cttgctaaaa taaattatca catacacaca gatgctataa agagcaattt
     1141 aattcctgaa aaggtatcaa aaacaaaaga atatttaatt tatgcaacag aagcagatat
     1201 tctaaatctt gctctttttg gattaactgc aaaagaatgg agagataata atcctaaaca
     1261 agaagggaat attagagatt atgcagatgt ttcacaatta gtgtgtcttt caaatttaga
     1321 aaacttaaat gcgttattta ttaaagaagg aataaataaa ttaaaaagat tagaaaaatt
     1381 aaacaatatt gcaattgagc aaatgataat cttaactaat caacatataa tcaagaaatt
     1441 aaaataagat ggtcctaccc gggctcggac cggggatctt taccatgtga aggtaacgtc
     1501 ataaccacta gactatagga cctatagttt tatttcaaat ggaaacaaaa ttaatttttt
     1561 aggcaataaa aatgttttat ttttaagacc tttttctgtt tttattatag tatttacaat
     1621 tatatatggt tcatggtttc tagccattct tgaaaaactg ttttcatcaa ttgttttaac
     1681 cattttttga aattttatat tcttttctaa aagaaaactt gatttcgata aaataatttt
     1741 tttacttatt ccttgtattt ttagtatatt atattctttt tttagagcat tatatgcttc
     1801 taatgcttta gaatccacag aaatacccat tctttctgca attctagggt gctctgcaaa
     1861 tatccaggtt tttccagtaa ttgtagaata tccgtttttt cttgctaaat ctataagccc
     1921 attaataatg attggtaaat atagatttgg attttttctt tttgataata ttgattctaa
     1981 ttcaagattc agcgcatttt tttcttttac atatagaacg tgattatcaa atgtaatttt
     2041 tgtggatttc tttggcttta tatttttagc agttttaaat tgcctttcta attttaatct
     2101 tgtaggatgc atattttctc aattattgta aattatatct ttttgctatt acacatctgc
     2161 gttttggttt cttggcagtg gagtatttca tgattgtaac attatcaaaa tatgaatcaa
     2221 agtatttttc tttacctgaa agatatttca taatctttgg ataaagcttt tcatttgatt
     2281 cttctgtaat tgttgtgtga agatgtattc catcgccttc aatattatta tgccaagtaa
     2341 gccattttag ttttcttagt tctttatgta aatcaaatat taatttcttt acttttggcg
     2401 attgactaac ctttacaaaa acaactcttt tatgaaaatt atcaaatcct ccaaccttta
     2461 ttttttcttt gttgtgaatt tttgaaaatt tcaataatgt tttttcaagt tcttttaatt
     2521 gtttagaatt tgccttaaat ttatacttca aggtaaagtg agcatagttg tttgctttct
     2581 ttgtgtgctt taaaccaaat tgtttgtata tttggtttac taaatctcta cgatatttat
     2641 ctatttcttt tttatgaagg caaaacaagc agtattccat tttttatctc cctttaagga
     2701 gttaatcttt caggatccct tggaagaaga actgcttctt tgacatttgc aaggtttagc
     2761 atcaactgtg tgattctgtc taaaccaaga cctgctccac catggcttgg aacaccataa
     2821 cttagcattt taacataata gtccatgtct tcaagtgaca ttcctttctc tgtaatctgt
     2881 tctttaagca ccgaggtcct atgttccctt tgagcacctg tacaaatctc gctgcctttc
     2941 cagattaggt ctgcgctttt ggtaatcttt tgtttagttt ctgggtctat ctcgcacatg
     3001 tgatagaaag gtcttttaga aaatggaaat tcagttaaga acacaaattc agaattgaat
     3061 ttctccttaa ctatatctcc aagcatcttt tcgccttcag agtctaggtc ttcttcagca
     3121 ggaatttctt tacctttagc ttttaggaga gctttagcgt ctttcatttt tattcttgga
     3181 atctttccag gtattttaat ttccaccttt aataattcta gttctgtttt acattctttc
     3241 tttacttcag ttagaacata cgccattagt tcttctagga cattatagac ttcttcaggg
     3301 tctttgatat agcccatctc taggtcaata ccagtaaact cagtcaagtg tcttgtagtg
     3361 tggctcttct ctgctctaaa cacttcgcca atctcataaa ccttattaaa accactagca
     3421 atcatcattt gcttatagac ttgaggagac tgggaaagga aagcttttct atcaaagtac
     3481 tttacttcaa ataactctga gccgctttct acgccgttag agaccatctt tggggtatta
     3541 atattaataa aacccttttc atcaaagaaa tcagtaactg ctctaacaat cttagatcta
     3601 actttaaaga ttgcaaggtt ttctggaact cttagatcaa tatatctgta attgattcta
     3661 gcatcaaaac cactatcaat ctttcctgag atatccaaag gcacaggggc atcagagatg
     3721 gaaagtattt caagagtctc aggaattact tcatagccat tcattgcttg ttctgaatcc
     3781 ttctttgttc cagtaacaat aatcacagac tctttagtaa ttgcctttgc tttttcaaac
     3841 atttcaggat tatccttctt aaagaaagta atttgttcct tcttgtattg atcccttaag
     3901 ataaagaaca tta
//
LOCUS       DAOOON010000045         3738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_29336_length_3738_cov_11.783600, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000045 DAOOON010000000
VERSION     DAOOON010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29336_length_3738_cov_11.783600"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLK55_04170"
     CDS             complement(<1..988)
                     /locus_tag="PLK55_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOZ36149.1"
                     /translation="MKYNIDLHIHSPYASGVSKNMSLPILAKEAKLKGLNVLGTGDIL
                     HPDWFKHVKENLVSDKDCYVFKDDKDSENKTYFILTTEVETSGRVHHLLFFRDFSQVL
                     EFKKDIEKFSSDMSVYGGGRPRLSIGPSTLLDLCIKHDVIIGPAHAFTPYFGIFSGND
                     SLKEAYGENYKEVRFIELGLSADSFSANVIPELKDLKLFSFSDAHSPNSYRIGREYVC
                     AEMEKPNFESIKNLLYNLGDNKILFNVGYNPQEGKYNRTACRDCKKIHKLEDAIKNNW
                     RCLKCRGILKKGVADRIIEIARLQGNSELKQIAKRPEYKYLIPLAQIIQMKIG"
     gene            1211..3502
                     /locus_tag="PLK55_04175"
     CDS             1211..3502
                     /locus_tag="PLK55_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36150.1"
                     /translation="MNNLQKIFGVILLLSLFIFPVYANDENSEEQMQINDENSEEQMP
                     EYELPGFDETQEPQLQVTFNLDLENINFDNQEHYSFSVSPKGDYQIIGFKYSFFDQSG
                     NSFGSIVINGFPTTKIVIGDLNFYDFEDGTITAKLTIITSGIDGEQTTKITKEITKKI
                     KVPIEIKANDISLENYENYLFKVKTEKIYTITGLNYTLKDHKHNLISEELDINETSSE
                     ITINNTNLSEFLDGEVTISVEVQIKNLYDEIKTVYLEKTITKDTSPIITVEAEDVTLE
                     NHEAYSFLIKAKRTYLITGLKHRIEDTEGELIAAATYLEDPISEIKPGHDLSDFADGT
                     ITITLEVKVLTPDGEILTIDIVREINKDTSPPIIITADHISLANHEGYSFNIKSKGRM
                     QILGVKYVITDSNNQIWPAAYLVDDPIYEMDTASHNLSHFVDGPVTIRLEIIIRIGGM
                     LGTTKRIDITKDIIKDTVVPILNLDQENIITNNNILTVTGEIEEFAELDSYIPFNENK
                     LKGKCDILPLEDGETLAHFSCVYDLTEIKDSLDYEIKFFAYDIVGNSTEKQASLIFDR
                     VAPIIVVTPLTTSDKTPILYGTIEDLTLVDFNIIVNDISYTPIVDENGNWSTQLEKLQ
                     RNRYYDVEIYAIDQAGNITSRTDLEMIHITNSGNGGGNGNNKGNDNGNNNDNDNGNDN
                     GNGPNLNFLPLSAPAPEAITEQATPNAVSAPEINDLIIEDQPKISGTTGLLSMPEKAS
                     KSIAIIIGALLVVGTLVYFILFN"
     gene            3556..>3738
                     /locus_tag="PLK55_04180"
     CDS             3556..>3738
                     /locus_tag="PLK55_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36151.1"
                     /translation="MNSKIKMLLGVFFVFALLSPIYAIETPTDVEAISVDDTNTSTDP
                     DLPHGDIEMPGILPDKP"
BASE COUNT         1397 a          561 c          600 g         1180 t
ORIGIN      
        1 tcccaatttt catttgaata atttgagcaa gtggtattag atatttatat tctggtcttt
       61 ttgcaatttg ttttaattca gaatttcctt gaagtcttgc aatttcaata attctatctg
      121 caactccttt ctttaaaatg cctctacact ttaggcatct ccagttattt tttattgcat
      181 cttctaattt atgtattttt ttacagtctc tacaagcagt cctattgtat ttcccttcct
      241 gcgggttata tccaacatta aataaaatct tgttatctcc aagattatat aataaatttt
      301 taatagattc aaagtttggt ttttccattt ccgcgcaaac atattctctt ccaatcctat
      361 agctgtttgg ggagtgtgca tctgaaaaag agaaaagttt taggtcttta agttctggta
      421 tgacgtttgc actaaaactg tctgcagaaa gtccaagctc aataaatctt acttctttat
      481 agttttctcc gtatgcttct tttaaagaat cgtttccaga gaatattcca aagtatggtg
      541 taaatgcgtg tgctggtcct attattacgt catgtttaat acacaaatct aaaagagtgc
      601 ttgggccaat agaaagtctt ggtcttccgc caccataaac actcatgtcg cttgagaatt
      661 tttctatatc ttttttaaat tctaaaactt gtgagaaatc tctaaaaaac aaaagatgat
      721 gtactcttcc agaggtttca acttctgtag ttaatataaa ataggttttg ttttctgagt
      781 ctttgtcatc tttaaagaca tagcagtctt tatctgatac aaggttttct ttaacatgtt
      841 taaaccagtc aggatgtaat atgtctcctg ttccaagaac atttaggcct tttagctttg
      901 cttcttttgc aagtattggt aaactcatgt ttttggatac tcctgaagcg taaggggagt
      961 gtatgtgtaa gtcaatatta tatttcataa taaaatatat gttaaaaacc tatataatag
     1021 gtgcgttagg taaaaagtca cttttataaa tgaacaggtt aaatattcaa actaggccgg
     1081 tttataaagg ttttctctca ctagtatata ttaagtattg acctaacaaa cttaatgtct
     1141 aattttacaa aacaaaaaaa gtttttagag atatattttt tttaaaaaca gtaattaaaa
     1201 aagtgagatt atgaacaatt tacaaaagat atttggtgtt attttattat taagtttatt
     1261 tatatttcct gtatatgcaa atgatgaaaa ttcagaagag cagatgcaaa taaatgatga
     1321 aaattcagaa gagcagatgc cagaatatga attgccaggc tttgacgaaa cacaagagcc
     1381 tcaattacaa gttactttta atttggatct tgaaaacata aattttgata atcaagagca
     1441 ctattctttt agtgtttctc caaaaggaga ttatcaaata attggtttta agtattcttt
     1501 ttttgaccaa tcgggcaatt cttttggaag cattgtaatt aatgggtttc caacaacaaa
     1561 aatagtaata ggcgacctta acttctatga tttcgaagat ggtacaataa cagccaaatt
     1621 aacaattatt acttccggaa ttgatggcga gcaaaccaca aaaattacaa aagaaataac
     1681 caaaaaaatc aaagtaccaa tagagattaa agcaaacgac ataagtttag aaaattatga
     1741 gaattattta tttaaagtta aaacagaaaa gatctacaca attacgggct taaattatac
     1801 tttaaaagat cataaacaca atttaatttc tgaagaatta gatattaatg aaacctcgtc
     1861 tgaaataact atcaacaata ctaatttatc agaattttta gatggagaag taacaatttc
     1921 tgttgaagta caaataaaaa atttatatga tgagattaag accgtgtatt tagaaaaaac
     1981 aataacaaaa gacactagtc caataataac agtagaagca gaagatgtaa ctttagaaaa
     2041 tcacgaggcc tattcttttt tgattaaagc aaaaagaaca tatttaataa ctggtctaaa
     2101 acacagaatt gaagatactg aaggagaatt aattgctgcc gcaacttatc tagaagaccc
     2161 aatctcagaa ataaagccgg gccacgattt gtctgatttt gcagatggca caattacaat
     2221 aacccttgaa gttaaagttt taactccgga cggagagatt ctgacaatag atattgttag
     2281 agaaataaat aaagatacaa gccctccaat aataattacc gcagatcaca taagtttagc
     2341 aaatcacgaa gggtattctt ttaatattaa atcaaaggga agaatgcaga ttcttggtgt
     2401 taaatatgta ataactgatt ctaataatca gatctggcca gcagcatatt tggttgatga
     2461 tccaatctac gagatggata cagcaagcca caatttgtct cattttgtag acgggccagt
     2521 aaccattcgt ttagaaataa taattagaat tggcggcatg cttggcacca ccaaaagaat
     2581 agatattact aaagacataa taaaagacac tgttgttcct attttaaatt tagatcaaga
     2641 aaacataatc actaataaca atattctgac agttacagga gagatagaag agtttgcaga
     2701 attagattct tatattccat ttaatgaaaa taaattaaaa ggaaaatgcg acattctgcc
     2761 attagaagat ggcgaaacat tagcacactt tagctgcgtt tatgatctta cagaaataaa
     2821 agattcatta gattatgaaa ttaaattttt tgcatatgat atagtgggca atagtacaga
     2881 aaagcaagct agcttaatat ttgacagagt cgctccaata atagtagtga ctccattaac
     2941 cacaagcgat aaaacaccga tattatatgg cacaatagag gatttaactt tagttgattt
     3001 caatataata gttaatgaca tatcttatac tccgatagtt gatgaaaacg gaaactggag
     3061 cacacaatta gagaaattac aaagaaatcg ttactatgat gtagaaattt atgcaataga
     3121 tcaagcaggt aatattacaa gcagaacaga tttagagatg atacacataa ccaattcagg
     3181 caatggcggg ggtaatggta ataataaagg taatgataac ggtaataata acgataatga
     3241 taatggtaat gacaacggga atggcccaaa tctaaacttc ttgcctttat cagcaccagc
     3301 gccagaagca ataacagaac aagcaacccc aaatgcagtc agcgcgccag aaataaatga
     3361 tttaattata gaagaccagc caaaaatttc aggcacaact ggtcttttaa gcatgccaga
     3421 aaaggcttca aaaagcatag caataattat tggggcttta ttagtagtag gcaccctagt
     3481 ttattttata ttatttaatt aatgtaagat agacaatata aaaatatcaa aaaataaaaa
     3541 ggagaaaaag aaattatgaa ttcaaaaata aaaatgttat taggtgtatt ctttgttttt
     3601 gcattattga gcccaatata tgctatagaa acaccaacag acgttgaggc aataagcgta
     3661 gatgacacaa acacatctac tgatccagat ttaccacacg gagatataga aatgccagga
     3721 attttaccag ataagcca
//
LOCUS       DAOOON010000046         3485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_32211_length_3485_cov_16.127405, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000046 DAOOON010000000
VERSION     DAOOON010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32211_length_3485_cov_16.127405"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..682)
                     /locus_tag="PLK55_04185"
     CDS             complement(5..682)
                     /locus_tag="PLK55_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36152.1"
                     /translation="MSEEKQLDYESLQLKERLEIHELQGLIGKALADSSKTEDDLIKI
                     IPNAKYEQIVQALKNMLFLKLITKEGFPVKYSLSPEVKEKLTEKKKLSENDKNVLKLA
                     VIIESKSNDKLELRKGMEHILESLRNDNEYFLYDSSLAEIVVHDDLFSTYISATLSCN
                     EINSLFKIIYFYGATSVEVLKPDKFNVTISDLQKAILIITDMTHGYAQMIHKLKRENE
                     ALSKLKK"
     gene            757..990
                     /locus_tag="PLK55_04190"
     CDS             757..990
                     /locus_tag="PLK55_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36153.1"
                     /translation="MDYLLLAIGVVLIAITILLLGLLKNIILNSIVGALGFLMCYFVF
                     GIKLPIVITLVVSALFGPAGLGVILLLRFFEII"
     gene            complement(1327..1602)
                     /locus_tag="PLK55_04195"
     CDS             complement(1327..1602)
                     /locus_tag="PLK55_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36154.1"
                     /translation="MRTTIEIKGLPELILEKAVSTGIARSKTDALKIGILIMNDKYQL
                     VDNIKEINDPKLIAAFKRKEKEMKANNQKYITHEEVMKKYGYLLKNK"
     gene            complement(1675..>1962)
                     /locus_tag="PLK55_04200"
     CDS             complement(1675..>1962)
                     /locus_tag="PLK55_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOZ36155.1"
                     /translation="INLKHIKENITELHKNKIHIEITNLLIEGHNTNISDIQETIDFV
                     YSVSKDIPLHFSRAFPHYRLNHIIPTKLETLKLAEKLAKDKGLVNVYLGNI"
     assembly_gap    1963..2062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2063..2368)
                     /locus_tag="PLK55_04205"
     CDS             complement(<2063..2368)
                     /locus_tag="PLK55_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019268052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOZ36156.1"
                     /translation="MFSKYFQVIDSNTIKCLACNRYCLIQKDKTGFCKVRKNENQKLV
                     SLVYGKPISINLDPIEKKPLYHFLSGTKTLSLGTPGCNFDCAFCQNHDISNCKNINLE
                     "
     gene            complement(2411..>3485)
                     /locus_tag="PLK55_04210"
     CDS             complement(2411..>3485)
                     /locus_tag="PLK55_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36157.1"
                     /translation="PDLPHGDIEMPGILPDKPDVIVDPIIRPELILNQTEIITKDNYI
                     LLTGSLKDLGVTVKYKIWGTTKTGNCRVQLTADTIEEAPEIVTFSAIDDQPSAISPIE
                     EDPIRPILPIVAERITFTCDLNVSDIEGKYKVDVIAENTERLNTTKKVDLIVDRTAPV
                     VDIDSISTRDRTPVLTGTIEDLIDIRKFKLTVNDVIYEPTINDDRTWEIQLETLPRGT
                     HNVEVYTSDRLSNADTKTEEEKITIRTGGGTRIVAEPTNTPTETVTPAPAPETPAAQP
                     VVTEPTITNTPTTPTTPTTGGTPITTPAPTTGNVAAVNPAETSGSTGFLGLPTGASTS
                     IALVIGALLVVGALGYFLFFAKK"
BASE COUNT          989 a          530 c          519 g         1347 t
ORIGIN      
        1 aaaactattt ctttaattta gaaagagcct cattttctct ctttagctta tgtatcattt
       61 gggcatagcc gtgcgtcata tctgttataa tcaatatggc tttttgtaaa tcagaaattg
      121 taacattaaa cttatctggt ttaagaacct caactgaagt tgcaccataa aaataaataa
      181 tcttaaataa ggagtttatc tcattacaag acaaagtagc agaaatatat gtagaaaaca
      241 aatcatcatg tactactatt tctgcaagac tactatcata taagaaatac tcgttatcgt
      301 ttcttaaaga ctctagaatg tgctccatgc cttttcgtaa ttccagctta tcattagact
      361 tagactctat gattactgct aattttagaa catttttatc attttcagac aatttctttt
      421 tctcggttag cttttcttta acttctggag acaaagaata cttaacagga aagccctctt
      481 ttgttattag ctttaaaaat agcatattct ttaaagcctg aacaatctgc tcatactttg
      541 catttggaat aatttttatt agatcgtctt ctgtcttaga agaatcagct aaggccttac
      601 caataagtcc ctgtagctcg tgtatttcta atctttcttt tagttgtaaa gactcatagt
      661 ctagttgttt ttcttcgctc atagttgctc actttttaaa gtatatataa cttataatta
      721 aaaaagtata taaaaggtta taatctaaga gaatatatgg attacttgtt acttgctatt
      781 ggagttgtgc ttattgcaat aactatttta ttattaggat tattaaaaaa tataatatta
      841 aacagcattg ttggagcttt aggcttttta atgtgctatt ttgtatttgg aatcaagttg
      901 ccgattgtta ttacacttgt tgtttctgct ttgtttggac ctgctggtct tggagttata
      961 cttttgctta gattctttga gattatttag cattaaagat aactcttata ccatttctca
     1021 tattgagtgt gattccccac aaaaacaata gttcttgttt tggtttcttt attttcaaaa
     1081 aaagcaattc tatattgccc cacctctgaa ataaaaaaat ttaacttgcg gaaatgtctt
     1141 gtatgtatat cgaactgtaa tttgtttatt ttcttaaaaa tgtgcgattg tattgtttga
     1201 tctaacctat taaaataaat gtcccagttt gcttcataat gtatagtcca tatacccata
     1261 aagatagaaa tagattatga tatataataa accgcgtgct ccacaatttt gtgaaattga
     1321 gaaaaattac ttgtttttta gtaaataacc atactttttc ataacttctt cgtgagttat
     1381 atatttctgg ttatttgctt tcatttcttt ctcttttctc ttaaaggcgg ctattaattt
     1441 tggatcattt atttctttta tattatctac gagctgatat ttatcattca ttattagaat
     1501 gccaatcttt aatgcatcag tcttagatct agcaatgcct gtagaaacag ccttttctaa
     1561 aatcaattca ggcaagcctt taatttcaat tgttgttctc atagttcttt ccttataatt
     1621 tgtgcatcaa ttcactatct atttagtgca tagaaatatt taaaaaaaga ttgcttaaat
     1681 attaccaaga taaacattaa caaggccttt atcttttgct aacttttctg caagctttaa
     1741 ggtttctagc tttgttggaa taatgtgatt tagacgataa tgtggaaaag ccctagagaa
     1801 atgcaaagga atgtctttag aaacagaata tacaaaatca attgtttctt gaatatccga
     1861 aatatttgtg ttgtgtcctt ctattaatag atttgtaatt tctatatgaa ttttattttt
     1921 atgtaattct gtaatattct cttttatgtg cttaagattt atnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnttctaaat taatattctt gcaattagaa atatcgtgat
     2101 tttgacaaaa cgcgcaatca aaattacagc caggtgttcc taaagacagg gtttttgtac
     2161 cagaaagaaa atgataaagc ggctttttct ctattggatc aagattaata gaaattggtt
     2221 taccataaac taaagaaaca agcttttgat tttcgttttt tctaacctta caaaagccag
     2281 tcttgtcttt ttgaattaaa caatatctat tacatgctag acattttata gtattagaat
     2341 caattacttg aaaatatttt gaaaacataa ataaaaaaag aaaaataaaa aaggagaaat
     2401 tcattccttt ttatttttta gcaaagaata ggaaataacc taatgcgcct actactaaca
     2461 aagcacctat aaccaacgca atgctagttg atgcacctgt tggcaatcct aaaaaacctg
     2521 ttgaaccaga agtctctgct ggatttactg ctgcaacatt acctgttgtt ggtgcgggtg
     2581 ttgttattgg tgttccgccg gttgttggtg ttgttggagt agttggtgtg tttgttattg
     2641 ttggttctgt tacaactggt tgagcagctg gtgtttctgg tgctggtgct ggtgttactg
     2701 tctctgttgg cgtatttgtt ggttctgcaa caattcttgt gccgcctcct gttcttattg
     2761 ttattttttc ttcttctgtt ttggtatcag catttgataa tctatcgctt gtataaacct
     2821 ctacgttgtg tgtgcctctt ggtaatgttt ctaattgtat ttcccaagtt ctgtcgtcgt
     2881 ttattgttgg ttcataaatt acgtcattta ctgttaattt aaattttctt atatctatta
     2941 aatcctcaat tgtacctgtt aatactggag ttctgtctct tgtagagatt gaatcaatat
     3001 caactactgg tgcagttcta tctacaatta aatcaacttt ttttgtagta tttaatcttt
     3061 cggtattttc tgcgataacg tctaccttgt atttcccctc tatgtctgag acattgagat
     3121 cacaagtaaa tgttattctt tcagctacaa ttggcaaaat tggtcgtata ggatcttctt
     3181 ctattggaga tattgcactt ggctgatcgt ctattgctga aaatgtaaca atctctggcg
     3241 cttcttctat ggtgtctgct gtaagttgta ctctacaatt gcctgttttt gtggtgcccc
     3301 atattttata ttttactgta acgcctaaat cttttaaact tcctgtaagt aatatataat
     3361 tatcctttgt aattatttct gtttgattta atataagctc tggtctaatt attggatcca
     3421 caattacgtc tggcttatct ggtaaaattc ctggcatttc tatatctccg tgtggtaaat
     3481 ctgga
//
LOCUS       DAOOON010000047         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_34039_length_3346_cov_9.883926, whole genome shotgun sequence.
ACCESSION   DAOOON010000047 DAOOON010000000
VERSION     DAOOON010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34039_length_3346_cov_9.883926"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1098
                     /locus_tag="PLK55_04215"
     CDS             229..1098
                     /locus_tag="PLK55_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36158.1"
                     /translation="MKKILVTSFVIFALLGLMTNIYANNSCTTVDVNGVLIEGCRSCI
                     DVNGNPCVDNNQTDTDDNETDTDDNETDTDDNETDINKEIGKFGSTQFAMKIKLIHLI
                     NTIDLKIAQAQSVVDAINDMNDSNVTIDTVPIATEITNLQDIKTRIQTTLDDKNLTKE
                     QILEVFLTEKENAKTAIKNIREYIRENCPIEMRNEFKIKFREQKKELKPEFHQRVKEL
                     RSQHNEFVKGEFREILGQTKKQLKIGPPKTKNIDSEELETEFQEKIREKKEQREINQK
                     LMQNRVKVRGGNN"
     gene            1098..1307
                     /locus_tag="PLK55_04220"
     CDS             1098..1307
                     /locus_tag="PLK55_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36159.1"
                     /translation="MKTKTVISVLVIVVLIVIAFLLFAPKADSNSGNNLTNNEEIIAD
                     QEAANETLVDDEILNPEVDIGELYE"
     gene            complement(1331..2245)
                     /locus_tag="PLK55_04225"
     CDS             complement(1331..2245)
                     /locus_tag="PLK55_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOZ36160.1"
                     /translation="MKTEIEIIERKGIGHPDTIADNLSEGLSKSLLKEYKTFKHFNVD
                     KTMVCAGEIEKGAFKNPVRIVFAGQYTKVNNIDKILDSVVKDILNFEIKNGLKYKIYN
                     FLNQGSPDLNDIFACKKANDTSFSVGYPETENERLVKKIGEYIDNLHKTDKHIGTDNK
                     VMFVYDGKEKSVYIALAFLDSFKDSKDYFSYKKKLEEKIKTKFKIKEIHINVGDTKDT
                     QFITKTGTSLEMGDSGATGRGNRRNGLITPTKIMTLEAYYGKNPITHIGRTYQDLAID
                     MAKKKNKHLLLLNKIGGDIKKPMVFELK"
     gene            2344..2544
                     /locus_tag="PLK55_04230"
     CDS             2344..2544
                     /locus_tag="PLK55_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36161.1"
                     /translation="MVTKKGIGEKMATEARVSPYFSKEQLKIIDGLINKIGCSRADVV
                     KQLVLIKLTELGYFNHPEKTRK"
     gene            2554..3099
                     /locus_tag="PLK55_04235"
     CDS             2554..3099
                     /locus_tag="PLK55_04235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOZ36162.1"
                     /translation="MEKYPYLKKPIETERLILVKPSLDFCKRKLEIWSNPKMTKYNEE
                     HLTLKEVTKRMKTNIKNQGKTAISFYALLKDNKYLIGSAGLNLSKHNNSAELGYGIDA
                     NYWGKGYCTEIVKAVVDYTFTKTDLHRIWATPVVENKASCKVLENCSFKKEATLRDSA
                     RHNGKYYNEVYYSIIRKNKKN"
     gene            complement(3153..>3346)
                     /locus_tag="PLK55_04240"
     CDS             complement(3153..>3346)
                     /locus_tag="PLK55_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36163.1"
                     /translation="FIAAIRDRYFDKEGYLNPKRERVNSIFKTYAENKKLAVLKLKKM
                     KQEKQKQKAIRERRKVKIK"
BASE COUNT         1256 a          513 c          483 g         1094 t
ORIGIN      
        1 actatctttt aaaagaactt aaaattccta aatctagtct ttctagaaat cttaagtctc
       61 ttgaaacgaa agatatcata gagataaaaa gatacggtat tactaacaaa atactactta
      121 aacacaagta gttccggttc tgttccggta tttcccggag aatttatatt aagagttcca
      181 atatactata tatagttata ataataaaaa atgttaggag gaaaaaaaat gaagaaaata
      241 ctagttactt catttgtaat atttgcatta ttaggattaa tgacaaatat ttacgcaaat
      301 aacagttgta caaccgtaga tgtaaatggc gttttaatcg aagggtgtcg ttcttgtatt
      361 gatgttaatg ggaatccgtg tgtagataat aatcaaacag acacagatga caatgaaaca
      421 gacacagatg acaacgaaac agacacagat gacaatgaaa cagacataaa taaagaaatt
      481 ggtaaatttg gatcaactca atttgctatg aaaattaagt taatacattt aattaataca
      541 attgatttaa aaattgcaca agcacaatct gtagtagatg caattaatga catgaacgat
      601 tcaaatgtca caattgatac ggtgccaatt gcaacagaaa ttacaaattt acaagatata
      661 aaaacaagaa tacaaacaac attagatgat aaaaatttaa caaaagaaca aatattagaa
      721 gtattcttaa cagaaaaaga aaatgcaaaa accgcaatca aaaatataag agaatatatt
      781 agagaaaact gcccaataga gatgagaaac gaattcaaaa ttaaatttag agaacaaaag
      841 aaggaattaa aaccagaatt ccaccaaaga gtaaaagaat tgagaagtca acataatgaa
      901 tttgttaaag gggaattcag agagatactt ggccaaacaa aaaaacaatt aaaaatcggc
      961 ccaccaaaaa caaaaaatat cgattctgaa gaattagaaa ctgaatttca agaaaaaata
     1021 agagaaaaga aagaacaaag agaaataaat caaaaattaa tgcaaaatag ggtaaaagta
     1081 agaggaggta ataattaatg aaaacaaaaa cagtaatttc tgtacttgtt atagttgttt
     1141 taatagttat tgctttctta ttgtttgcac caaaggctga ttcaaactct ggaaacaatc
     1201 taactaataa cgaagaaata attgctgatc aagaagcagc aaacgaaaca ttagtagatg
     1261 acgaaatcct taatccagaa gtagatattg gagaacttta cgaataagta aagtttcccc
     1321 ttttcttttt ttattttaat tcaaaaacca ttggtttctt gatatctcca ccaatcttgt
     1381 ttaataaaag tagatgttta ttttttttct ttgccatatc aattgcaaga tcttgatagg
     1441 ttctaccaat gtgcgttatt ggatttttac catagtatgc ttctagagtc ataatctttg
     1501 taggcgtgat taaaccattt cttctgtttc ctctacctgt tgcaccacta tctcccatct
     1561 ccaaagaagt tcctgtttta gttatgaatt gtgtgtcttt tgtgtctcca acgtttatgt
     1621 gtatttcttt tattttaaat ttagttttaa ttttttcttc tagtttcttc ttataagaga
     1681 aatagtcttt actatcttta aatgaatcca agaaagctaa agcaatataa acagactttt
     1741 ctttaccatc ataaacaaac ataactttgt tgtctgttcc tatgtgcttg tctgttttat
     1801 gaagattatc aatatattct ccaattttct taactaatct ttcattttct gtttctggat
     1861 aacctacaga gaaactagta tcatttgcct tcttacaagc aaaaatatca ttaaggtctg
     1921 gacttccttg gttcaagaag ttataaattt tatattttaa tccatttttt atttcaaaat
     1981 tcagtatgtc tttaacaacg ctgtctaaaa tcttatcaat attatttact ttggtatact
     2041 gacctgcaaa aacaattctt actggattct taaatgcgcc tttttcaatc tctccagcac
     2101 acaccattgt tttatcaaca ttaaagtgtt taaatgtttt gtattctttt aacaaacttt
     2161 ttgaaagccc ctcagatagg ttgtctgcaa tagtatcagg gtgtccaatt ccttttcttt
     2221 ctataatctc tatttctgtt ttcatgtttt ttctcctttg ttattatgta cttattagta
     2281 actaatgagt gtttagaaat atttaaaaag acttccgctc attagtaact gtatagcacc
     2341 cgtatggtta ctaaaaaagg aattggtgaa aaaatggcaa cagaagcaag agtgagtcct
     2401 tatttttcta aggaacaatt aaagattata gacggattaa taaataagat tggttgctct
     2461 agagcagacg tggttaagca attagtatta atcaagttaa cagaattagg gtatttcaac
     2521 caccccgaaa aaacacgcaa atagttggac aacttggaaa aataccctta tctcaaaaaa
     2581 ccaatagaaa ccgaaagatt aatacttgta aaaccctctc tggatttttg taaaagaaaa
     2641 ctagaaattt ggtctaaccc aaagatgact aaatacaatg aagaacacct tactcttaaa
     2701 gaagtaacta aaagaatgaa aacaaatatt aaaaatcaag gcaaaactgc gatttccttc
     2761 tatgctcttt taaaagacaa caaatatttg attggctctg caggcctaaa cctctcaaaa
     2821 cataacaact ctgcagaatt aggttatgga atagatgcaa actattgggg gaaagggtac
     2881 tgcacagaaa ttgttaaagc agtagtggat tatacattca caaaaaccga tttgcacagg
     2941 atatgggcca ctcccgttgt tgaaaacaaa gcatcttgta aagtgcttga gaactgcagc
     3001 tttaaaaaag aagcaacact tcgagattct gcaaggcaca atgggaaata ctacaatgaa
     3061 gtttactatt caatcattag aaaaaataaa aaaaattaag cagttacttt ttttttcatt
     3121 aatggcataa tatcacacaa atacttggac gactatttga tttttacttt tcttctttct
     3181 ctaattgctt tttgtttttg tttctcttgt ttcatctttt ttaattttaa tactgccaac
     3241 tttttatttt ctgcataggt cttaaaaatg gaatttaccc tttctctttt tggattaaga
     3301 tatccttctt tatcaaaata tctatcccta attgctgcaa tgaacc
//
LOCUS       DAOOON010000048         3312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_34485_length_3312_cov_16.185140, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000048 DAOOON010000000
VERSION     DAOOON010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34485_length_3312_cov_16.185140"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLK55_04245"
     CDS             complement(<1..943)
                     /locus_tag="PLK55_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36164.1"
                     /translation="MFKSKKKAQISIEALILVGIIVLGATIFASMYLNRIARGISDST
                     NLDSGLSDLFYDPFGDGTGSCSGGYPLHCCNETQDSGEDAIDCGGECPGCPIPPNTFS
                     IDLTLNPLGSTPINQEFGVTVTLTNTTGESGAIIKAVNIKEGFALTPNCTLSGQTQES
                     TGYYNNVNISLDSGAGTLNFTCSAEGTYNISVDAGIPDTTYSDSKATEKIITSGFSPA
                     YSVKIIEPAEGAYLAMEKQVNFRASITPTPLAGENDSCVWFVNDELLTPLMPASNSCS
                     LENIMLSEPTFKKGENEIVVYATRRVGGVMTNNAKASV"
     gene            complement(1065..>3312)
                     /locus_tag="PLK55_04250"
     CDS             complement(1065..>3312)
                     /locus_tag="PLK55_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOZ36165.1"
                     /translation="PHARILRNYISNPVDGKYFGNIAAVPELGYYGNQFEVHVATHEF
                     AHVLGLIDLYAIGPDGSGDGPIKNFTMMNFEFHPYCLNKPINLDVWSRYFLGWIEPMI
                     LTTDSSKEISLRSVNYYRDAVILKNSNMTDREFFIIENRYRNTSDPNNLDNCMFNNAQ
                     TTLGGFAIYHVDENKIEAHYPYNWVNWDPDGNWYDDTISHPGILYERNYTYSYSGGNY
                     STGDMYFNAIVQGCDSFRFFDENKRICTNIYAIDDSTTRTYSGVENPLIRFQALTTPN
                     QSVMTAKMLVWEETETPQSSPPAGTYPQSTQVILSTATPNSIIYYTTDGSIPTQDSQV
                     YINPITIPEGTTQTIKAIAKKDGYYISNIMSSTYTITGTVVTPTASQQSGIVDYGSQV
                     ILNTTTNGATIRYTLDGSNPTETSLIYNSPIIITESLTLKAKAFKLGYTPSSVSTYEY
                     QVLVVEAPISTLASGYYDYGTNLELYTTTENAEIRYTIDGSEPNENSLLYTNQITILN
                     DFTLKAKGFKQSYAPSQTSTYIYDSGPAPGNHIISFGFQGIDSQAEIDNDQNIILVYV
                     PAGTYCYGLIPIIQVEDGCSVSPNNGVPQNFIEPVIYTVSSMSGQRVYTVNVIRNPQV
                     FPPIANISGGDFGAPIDVELTTQTVGAIIKYTTNGVNPTLDSQTYTGPITISSTTTLK
                     AKAFLYTGETPELDSSKNNEDPKAIADITPTNVIWQSSATLIENYNFLQKHKIKNKKD
                     KPIFSNDK"
BASE COUNT          980 a          586 c          538 g         1208 t
ORIGIN      
        1 gcacacttgc ttttgcgtta ttagtcatta ctccccctac acgcctggtt gcataaacaa
       61 caatttcatt ttcacctttt ttaaaagttg gttcactcaa cataatgttt tctaaactac
      121 aagagtttga tgctggcata agtggagtaa gtagctcatc attaacaaac catacacaag
      181 aatcattttc tccagctaaa ggagttggtg taatacttgc tctaaaattt acttgttttt
      241 ccatagctaa ataagcgccc tcagctggtt ctataatttt tacagaatat gctggtgaaa
      301 aaccagaagt aattattttt tctgtagctt tggaatcgga ataggtcgtg tctggtatcc
      361 cggcatccac agaaatatta taggttcctt ctgcagaaca ggtaaaattt aaagttcccg
      421 cgccagaatc caatgagata tttacattat tataataccc tgtactttct tgcgtttggc
      481 cacttaaagt acaattaggg gtcaaagcga acccttcttt tatatttact gccttaatta
      541 ttgctcctga ttcccccgtg gtattagtta aagtaacagt tactccaaat tcttggttta
      601 taggagtaga acctagaggg ttaagagtta aatctattga aaaagtatta ggaggaatcg
      661 gacatccagg gcattcacca ccacaatcta tagcgtcttc accactatcc tgtgtctcat
      721 tacaacaatg taatggataa cccccagagc aagaccctgt gccgtcgcca aacggatcat
      781 aaaaaagatc gcttaatcca gaatctaaat tcgttgaatc gcttattccg cgcgcaattc
      841 tattcaaata cattgacgca aatatcgtgg cacctaaaac tattatccct actaaaatta
      901 gtgcttctat cgaaatttgt gccttttttt tagatttaaa catatttgcc cacggctcta
      961 aaaattttat attttaatta tattgtaaaa caatcaattt ataaaggttg cttttgtttt
     1021 taagcccatt attattaaaa ttacccaaat gcttaaaatc acatttattt gtcattacta
     1081 aatattggct tatctttttt gtttttaatt ttgtgctttt gtagaaaatt ataattttct
     1141 attaatgtgg cgctactttg ccaaataacg tttgttggtg taatatctgc tattgctttt
     1201 gggtcttcat tatttttaga agaatctaat tctggcgttt ctccagtata taaaaacgct
     1261 tttgctttta atgttgttgt tgagctaata gttattggcc ctgtatatgt ttgactgtca
     1321 agtgttggat tgaccccgtt ggtggtatat ttaataattg ctccaacagt ctgtgtagtt
     1381 aattctacat ctattggcgc accaaaatct ccgccagaga tatttgcaat tggtggaaac
     1441 acctgtggat ttcttataac attaacagta taaactcttt gaccagacat tgaacttact
     1501 gtataaatta ctggctcaat aaaattttgt ggaactccgt tattcggaga aacactacag
     1561 ccatcttcga cttgaattat tggtattaat ccatagcaat atgttcctgc tggaacatat
     1621 actaaaatta tattttgatc attatcaatt tctgcttggc tatctattcc ttgaaagcca
     1681 aaggaaataa tgtggtttcc tggtgctggc ccagaatcat aaatataagt tgatgtttgg
     1741 cttggggcat aactctgctt aaagcctttt gcttttaatg taaaatcatt taaaattgta
     1801 atctgattag tatatagtaa actattttca tttggttcac taccatcaat tgtatatctt
     1861 atttctgcat tctctgttgt tgtatacaac tctaaatttg tgccgtaatc ataatatcca
     1921 gaagctaaag tagatattgg agcttctaca actaaaactt gatactcata agtagaaaca
     1981 gaacttggcg tatatcctaa tttaaaggcc tttgccttta aagttaagct ttctgtaatt
     2041 ataattggag agttatatat taaactagtt tctgttggat tgctgccatc aagagtatat
     2101 cttattgttg caccatttgt tgtggtattt aaaattactt gagaaccata atcaacaatt
     2161 ccgctttgtt gactggccgt tggtgttaca acagtacctg ttattgtgta agtacttgac
     2221 attatattac ttatataata gccatctttt tttgcaatag cttttattgt ttgtgtggtt
     2281 ccttctggaa tagttattgg gttaatatat acttggctat cttgtgttgg tatagaacca
     2341 tcagttgtat aataaattat agaatttgga gttgctgtac ttagaataac ttgagttgat
     2401 tgtggataag tgcctgctgg tggagaagat tgcggagttt ctgtttcttc ccaaacaagc
     2461 atctttgcag tcattactga ttgatttggg gttgttaaag cttgaaaacg aattaaagga
     2521 ttttctacac cactatatgt gcgagttgtt gaatcatcaa ttgcataaat atttgtacag
     2581 attcttttat tttcatcaaa gaatctaaaa gaatcgcaac cttgtactat tgcattaaaa
     2641 tacatatccc ctgtactgta attgccgccg gaataactat atgtataatt tctttcatat
     2701 aatattcctg ggtggcttat tgtatcatca taccagttcc cgtctggatc ccaatttacc
     2761 caattatatg ggtaatgcgc ctctatctta ttttcatcaa catgataaat tgcaaaacca
     2821 ccaagtgttg tttgcgcatt attaaacata caattatcaa gattgtttgg atcgcttgtg
     2881 tttctgtatc tgttttcgat tatgaaaaat tctctgtctg tcatattgct attttttaga
     2941 attactgcgt ccctataata atttacactt cttagactta tttctttaga gctatctgtt
     3001 gttagaatca ttggctctat ccaccctaaa aaatatcttg accaaacatc aagattaatt
     3061 ggcttgttaa gacagtaagg gtgaaattca aaattcatca tcgtaaaatt ttttattggg
     3121 ccatctcctg aaccatctgg gccaatagca taaaggtcta ttaaaccaag aacatgtgca
     3181 aattcgtgtg ttgctacatg aacctcaaat tgatttccat aataaccaag ctctggaact
     3241 gccgcaatat ttccaaaata tttgccatct acaggattac ttatataatt tcgcagaatt
     3301 cttgcatgtg gc
//
LOCUS       DAOOON010000049         3018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_39185_length_3018_cov_15.527168, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000049 DAOOON010000000
VERSION     DAOOON010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39185_length_3018_cov_15.527168"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1174
                     /locus_tag="PLK55_04255"
     CDS             176..1174
                     /locus_tag="PLK55_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011271318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOZ36166.1"
                     /translation="MISIIDNDYFITLNEIKDKIRNSQIKATISVNKELIKLYLNIGK
                     IIVDKQKTSNWGDSIISILSKDLMKEFPDMKGFSRSNLFNMRKFYLSYCLFEPKVQQL
                     VGQLPWGHNIILIDKIKNNTEIKFYVFEAIKNNWSRNTLINQIENNLFQRSGKVINNF
                     NKTLPKIQSDLAVQTIKDPYIFDFLEISKKAKEKDIEEKLVNNITKFLLELGVGFSYV
                     GKQYHLVVENEDFYIDLLFYHLRLRCFIVIELKSGRFKPEYVGKLNFYLSALDRLKHK
                     NDNSSIGLLLCKEKNKIIAEYSLKDISKPIGVSQYKLSKKLSKVLPTKEELEKIIE"
     gene            1255..2088
                     /locus_tag="PLK55_04260"
     CDS             1255..2088
                     /locus_tag="PLK55_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOZ36167.1"
                     /translation="MQEKNEIAIFENKKIRRIWHEEDWWFSVVDVVGALTDSEDPRKY
                     WNKLIQRLREENSEVVTNCHQLKLIAEDGKFRKTDCANLEGVFRIIQSIPSPKAEPFK
                     LWLAKVGYQRIEENIDPELAINRAVNNYLKKGYSVEWVNQRLKAIEVRKELTDEWKRS
                     GIDKPSEFASLTNTLTKAWSDRTVREYKDLKGLKKENLRDNMTNIELVLNLLAEATTT
                     EISKVEDPKGFLPSENIAKSGGEIVGTTRRNIEKRLGKTVITSKNAKNLLAEKKKKQI
                     T"
     gene            complement(2081..2734)
                     /gene="nth"
                     /locus_tag="PLK55_04265"
     CDS             complement(2081..2734)
                     /gene="nth"
                     /locus_tag="PLK55_04265"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOZ36168.1"
                     /translation="METKDIRRIYSILEKEVKKFDNPFAESVQEKYKDPFFVLVATIL
                     SARTLDKTTAKVCEKLFKEVKGINDLEKISISKLEKLLYPVGFYKTKAKHLKELPIVL
                     KERYNGRIPEEIEQLLELPGVGRKTANLVVSVTFGKPGICVDTHVHRITNILGIVKTK
                     TALETEMALRRILPKELWTKTNYLFVTLGQNICFSRKPNCRMCPIAKMCPFGLKKIK"
     gene            complement(2768..>3018)
                     /locus_tag="PLK55_04270"
     CDS             complement(2768..>3018)
                     /locus_tag="PLK55_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36169.1"
                     /translation="FFWRDAYKNEVDMIFEKNKQIIPIEIKYGKIETAGLDKFMSNYK
                     IDVGYIISHNTEKEIKKDKKIIYVVPAYKFLLNKDKYL"
BASE COUNT         1155 a          427 c          440 g          996 t
ORIGIN      
        1 tgacacagaa gataaaacaa aagttagcct taactaactt tttctttttc ttatttttgt
       61 gaaaatgaaa ttttcttata gttaattctc aaaaaaacaa caaagattta tatattttgc
      121 actggacagc tacccctcca aaaacacccc ctctttctaa agtcacaata tatttatgat
      181 ttcaataatt gacaacgatt attttattac attaaatgaa ataaaagaca aaataagaaa
      241 ttcacaaatt aaagcaacta tttctgtaaa taaagaatta ataaagttgt atttaaatat
      301 cggtaaaata atagtggata aacaaaaaac ctcaaattgg ggggattcaa taatatctat
      361 attatccaaa gatttaatga aggaatttcc agatatgaaa gggttttcta ggtctaattt
      421 atttaacatg aggaagtttt atttgtctta ttgcttattt gaaccaaaag tccaacaact
      481 tgttggacaa ttgccttggg ggcacaatat aatattaatt gataaaatta agaataacac
      541 agaaattaaa ttttatgttt ttgaagcaat aaaaaacaat tggtcaagaa atactttgat
      601 taatcaaatt gaaaataatt tatttcagcg atcaggaaaa gtaataaata attttaataa
      661 aaccttgcct aaaatacaat ctgacttagc tgttcaaact ataaaagacc catatatatt
      721 tgatttttta gaaatatcta aaaaagctaa agaaaaagat atagaagaaa aactagtaaa
      781 taatattact aaatttttac tagagttagg agttggcttt tcatatgttg gtaaacaata
      841 tcatttagtg gttgaaaatg aagatttcta tatagattta ttattttatc atctaagatt
      901 aagatgtttt atagttatag aattaaaatc cggaagattc aaaccagaat atgtaggaaa
      961 attaaatttt tatttatctg cacttgatag attgaagcac aaaaacgata attcttctat
     1021 tggattacta ttatgtaaag aaaaaaacaa aataattgca gaatattcgt taaaagatat
     1081 ttctaaacca attggcgttt ctcaatataa gttatctaaa aaattatcaa aagttcttcc
     1141 aacaaaagaa gaattagaaa aaattataga ataattttta atttttgcac tggacagcta
     1201 ctcccccgaa aacacccctc tttctttcac caatatatta ttggtgactc ttaaatgcaa
     1261 gaaaaaaacg aaattgcaat ttttgaaaat aaaaagataa gaaggatatg gcacgaggaa
     1321 gactggtggt tttctgttgt ggatgttgta ggagcattga ctgatagcga agaccctaga
     1381 aagtattgga ataagttaat acaaagactt agagaagaaa atagtgaagt ggtgacaaat
     1441 tgtcaccagt tgaaattgat agccgaagac ggcaaattta gaaaaacgga ttgtgctaat
     1501 cttgaaggtg ttttcaggat tatccaatca attccatccc ccaaagccga accgtttaag
     1561 ctatggcttg caaaagttgg ttatcaaaga attgaagaaa atattgatcc agagcttgca
     1621 atcaatagag cagtaaataa ttatctaaaa aaaggatatt cagtagaatg ggttaatcaa
     1681 agattaaaag caatagaagt tagaaaagaa ctaacagatg aatggaaaag gtcaggaata
     1741 gataaaccat cagagtttgc atctctaaca aacactttaa caaaagcctg gtcagataga
     1801 acagtaagag aatataaaga tttgaaaggt ctaaaaaaag aaaacctaag agataatatg
     1861 actaacattg aacttgtcct taaccttcta gcagaagcca caactaccga gatttcaaaa
     1921 gttgaggatc caaaagggtt tctaccaagc gaaaatattg ctaagtcagg cggagaaata
     1981 gtaggaacca caagaagaaa tatagaaaaa agattaggaa aaaccgtaat aacttctaaa
     2041 aatgcaaaaa atcttttagc agagaaaaag aaaaagcaaa tcacttgatt ttcttaaggc
     2101 caaagggaca catcttagca atagggcaca tcctgcaatt tggttttcta gaaaagcaga
     2161 tattttgtcc aagtgttaca aaaagatagt ttgttttagt ccacagttct tttggcaaaa
     2221 ttcttcttaa agccatttct gtttcaagtg ccgtttttgt ttttacaatt cctaagatat
     2281 tagtaatcct atgcacatgt gtatccacac agattcctgg cttcccaaag gtcacagaaa
     2341 caactaagtt agcagttttc cttccaactc ctgggagttc aagcaattgt tcaatttctt
     2401 cagggattct tccattatat ctttctttca aaacaattgg aagttctttt aagtgcttag
     2461 cctttgtctt ataaaatcca acaggataaa gtaatttctc taatttgcta atggatattt
     2521 tctcaagatc attaattcct ttaacttctt taaatagttt ctcacaaacc tttgcagtgg
     2581 ttttatcaag tgttcttgca gataaaatgg ttgccactaa gacaaaaaaa ggatctttat
     2641 atttctcctg aacagattca gcaaaagggt tatcaaactt ctttacttct ttttcaagaa
     2701 tagaatatat tcgtctaata tctttagttt ccattttttt atcatttctt aaaagtaatt
     2761 ttacagttta tagatatttg tctttattta ataagaactt atatgcagga actacataaa
     2821 taattttttt gtctttttta atctcttttt ctgtattatg gctgattata tatccaacgt
     2881 caattttata atttgacata aatttatcta atccagcagt ttcaattttt ccatatttta
     2941 tttctattgg tataatttgt ttgttttttt caaaaatcat atctacttca tttttataag
     3001 cgtctctcca aaagaaac
//
LOCUS       DAOOON010000050         2982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_39856_length_2982_cov_13.914588, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000050 DAOOON010000000
VERSION     DAOOON010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39856_length_2982_cov_13.914588"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLK55_04275"
     CDS             complement(<1..668)
                     /locus_tag="PLK55_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOZ36170.1"
                     /translation="MKIEVNFPDGSIKQVEAGRKAKDIILENIGEGLARIAIAVKANG
                     KLIDLETPITTTTDFLVLTYKDLEGKEIFWHSSSHIMALAIKRLFPEVKFAIGPSIDE
                     GFYYDVDINRAFTEEELQKIEEEVNKIIKEDIKFERHEIDFNQALEIFKDEPYKIEMI
                     KEIKDKGESLSYYQLGDFIDLCRGPHIPSSGKIKAFKVLKASGAYWRGDSKNKQLQRI
                     YGVS"
     gene            complement(882..1076)
                     /locus_tag="PLK55_04280"
     CDS             complement(882..1076)
                     /locus_tag="PLK55_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HOZ36171.1"
                     /translation="MSDDYRGPRGNDRGNRGNFHRNTGPREMHKATCSKCGKECEVPF
                     KPLEGKPVYCRDCYMSMKNQ"
     gene            1374..1646
                     /gene="ffs"
                     /locus_tag="PLK55_04285"
     ncRNA           1374..1646
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLK55_04285"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            1853..2956
                     /gene="ftsZ"
                     /locus_tag="PLK55_04290"
     CDS             1853..2956
                     /gene="ftsZ"
                     /locus_tag="PLK55_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOZ36172.1"
                     /translation="MKSLVNNAVSAKSNTNADYSKDGEFAAPKIMVIGVGGAGNNSVN
                     RISAMGVAGAELIAVNTDRAHLGIISDDITKLLIGKSVTNGLGAGAYPEVGAKAAEVS
                     KAALEEILDGVDLLFITAGMGGGTGTGAAPVVAQIAKQQGAIVVAIVTYPFALERARM
                     KKAEEGIDILKDICDTVVVIDNNRLVELVPNLPVQDAFKVADEVIARAVRGITETITQ
                     PSLINLDYADIRTVMGGKGLSVIAVGESQSVNKVDEVVEDTLKNALLDVDITGATGCL
                     IHITGGTELTLGEANAVGEMLTEQIDPNAQVIWGARIDPTFENKLEVIAIFTGVKSPF
                     IKGSKKFTGGNQPASNYGRGSGRYNDDFGIDML"
BASE COUNT          993 a          471 c          527 g          991 t
ORIGIN      
        1 taagaaacac catagattct ttgaagttgt ttgttcttac tatctcctct ccagtaagct
       61 cctgatgctt ttaatacttt aaatgctttt atttttcctg aagatgggat gtgagggcct
      121 ctacaaagat caataaaatc tccaagttga taatatgaaa gagattcgcc tttgtctttt
      181 atttctttaa tcatttctat tttgtaaggc tcgtctttaa agatttctaa agcttgattg
      241 aaatcaattt cgtgtctttc aaacttaatg tcttctttaa ttattttatt tacttcttct
      301 tcaatctttt gtaattcttc ctcagtaaat gctctattga tatctacgtc atagtagaag
      361 ccttcatcaa tgcttgggcc aatagcgaat ttaacttctg gaaacaacct tttgattgct
      421 aatgccataa tgtgtgaaga actatgccag aagatttcct taccttctaa atctttgtaa
      481 gttaatacta aaaaatctgt agttgtggta ataggggttt ctaagtctat caactttccg
      541 ttggctttaa cagcgattgc tattcttgct aatccttcgc caatattttc aaggataatg
      601 tcttttgctt ttcttccggc ttctacttgt ttaatagaac catcaggaaa atttacttct
      661 attttcatgt tttttcctcc gttttttgac acctaaaaaa tacctattac cacatagtta
      721 attttaggta aggtataata tattatataa aacagaaata aatataaata aacaagaata
      781 atacaatatt aaaaaaaata gtaaattaaa aaaagaaaaa aaattgattc tatttttaga
      841 atcaatttag atttttatat tgctgttaag aataagttct tttactggtt cttcatactc
      901 atataacaat ctctacagta aactggcttg ccttcaagtg gtttgaatgg aacttcgcat
      961 tccttaccgc actttgaaca agttgcttta tgcatttctc taggtcctgt atttctatgg
     1021 aaatttcctc tatttcctct atcatttcct cttggacctc tgtagtcatc actcatattt
     1081 ttttcctccg aaaaactaaa ctatatgtat gaagaactta tttctaatct ttatacatat
     1141 tactattaat attaattata gaaggtatta taaagctttt tcttttaaaa aaatagaaga
     1201 atctatagat tttcgcgttt tatttctgtt ttttatatta aaacctaaaa aaaataagtc
     1261 aaaataacgc ccccacaaag gattagcgca caaataacac tttttattaa aattatctga
     1321 atattggatg tttagaaaaa agaatagtta tttaaatagt ttcctaccta attatattgg
     1381 acagactggg gggcttgcgg tctcttgtaa ccagaaatcc gcatatgctg gggttatgcc
     1441 tagggcaagg ggaataagca atgttttgcc ctttgtaagg ttgccgacga ttcgtccatt
     1501 aagagtggat gatttagcta accctgtgag gccaggaatg gagcagcagt attctagaca
     1561 cccattgctt agtgggttgc ggatccgaga tcttatgaag tttcagcaga atattggact
     1621 gtttttcgaa ctttaggcgt ccatattttt cttattgtac tttttctaat ttaaaattta
     1681 ctaaattaaa taatagtatt taaaaacctt ttggttcata tacttataat taaaatgtat
     1741 ttttccaaga ataaagcaaa aggagcgtat tttgagcttc tttttattct gttaaaggat
     1801 ttctgtttga gaagttctat tgtggaaaaa gaaaaaatga ggtgtaaaaa acatgaagtc
     1861 attagtaaat aacgcagtta gcgctaaaag taatacaaac gctgactatt caaaagatgg
     1921 agaatttgca gcaccaaaga ttatggtaat aggtgtcggt ggtgcaggaa ataactctgt
     1981 aaacagaatc tcagcaatgg gagtagctgg tgcagaatta atagctgtta acacagatag
     2041 agcacactta ggtattatct cagatgatat cacaaagtta ttaatcggta aatcagtaac
     2101 aaacggactt ggtgcaggtg cttacccaga agttggtgca aaggctgctg aagtatccaa
     2161 ggctgcatta gaagaaatat tagatggtgt tgaccttttg ttcatcacag ctggtatggg
     2221 tggtggaaca ggtacaggtg ctgctccagt agttgctcaa atagctaaac aacaaggcgc
     2281 aatagttgtt gcaatcgtaa catatccttt cgctttagaa agagcaagaa tgaagaaagc
     2341 agaagaagga attgacatct taaaagacat ttgtgataca gttgttgtta tcgataacaa
     2401 cagattagtt gaattagttc caaacttacc agtacaagat gcattcaagg ttgctgatga
     2461 agtaatcgca agagcagtaa gaggaattac tgaaacaatc acacaacctt cattaattaa
     2521 cttagattac gcagatatta gaacagtaat gggcggtaaa ggattatctg ttattgctgt
     2581 tggtgaatca caatctgtaa acaaagttga tgaagtagtt gaagacacat taaagaacgc
     2641 attacttgat gttgatatta caggagcaac aggatgttta atacacatca caggtggaac
     2701 cgaattaaca ttaggtgaag caaacgcagt tggtgaaatg ttaacagaac aaattgatcc
     2761 taacgctcaa gttatttggg gtgctagaat cgatccaaca ttcgagaaca agttagaagt
     2821 aatcgcaatc tttacaggcg taaagtcacc atttatcaaa ggaagtaaga aatttacagg
     2881 tggcaatcaa ccagcaagta attatggtag aggatctgga agatacaacg atgacttcgg
     2941 catcgacatg ttataagtga gaaatcacac aatacaccaa ag
//
LOCUS       DAOOON010000051         2435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_52822_length_2435_cov_9.245378, whole genome shotgun sequence.
ACCESSION   DAOOON010000051 DAOOON010000000
VERSION     DAOOON010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52822_length_2435_cov_9.245378"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLK55_04295"
     CDS             <1..290
                     /locus_tag="PLK55_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HOZ36173.1"
                     /translation="KKRYLAIELLTYPQGLDSKTIYQSFSRALQKQSGIFVQLETNIT
                     IIEMDSKTKTILIRVNKIYLDKFVASLFFAQQDLGLIKVKNIKSTIKKINI"
     gene            319..816
                     /locus_tag="PLK55_04300"
     CDS             319..816
                     /locus_tag="PLK55_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36174.1"
                     /translation="MDVKNTTPESQKSNLLIAVKGISIYYGFVSLFYLILGTAIVFFS
                     KKIITFLTSNVKVDSANYLTVSKYLTVSNAVIIGGILLSIGLFILFSAIGLWKLKNSA
                     RIYLIIVTIFSCVSFFFWGVFSIFSLVFNIFILNILFKSKKLFISKDDLETTNVDLNK
                     KEVWR"
     gene            821..1573
                     /locus_tag="PLK55_04305"
     CDS             821..1573
                     /locus_tag="PLK55_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOZ36175.1"
                     /translation="MIGAVIIIFLVIGIVVFFSGLLQVYQFQRAVVFTLGKFSHVKEP
                     GLGWIVPIIQRARIIDMRIKTADVPRQEVMTKDNIPVLANTVVYFKVEKPEDVVIKIE
                     DFEYAVRQYTQAALRDVIGNAELDFVLSEREKIANSIKEIVDAETTDWGVDIESIKIQ
                     EIELPSEMKRAMAKQAEAERERRAVIIAADGELKASENLKKAADNLATNPVAIQLRTL
                     QTIRDIAADPSEKIVLFMPTEIVETVKKIVKK"
     gene            1595..2344
                     /locus_tag="PLK55_04310"
     CDS             1595..2344
                     /locus_tag="PLK55_04310"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOZ36176.1"
                     /translation="MFFENLRRISLDLNGQTSNIYQPRRAPKKFDTKKRYLQLAFNSS
                     TAQAINFISQLEYNERIIIEVGTPLIKKEGVSAIRTIRNYWPGFIVADIKIADGAEQE
                     VEYVYREGADAVTVLGCTNKETIDYCISVCKKYNLISMVDMIGVSEPHRVLLSLKKQP
                     DVIILHKARDQEHIKGFKIPYKQVNKIRSKYGALISAAGGVDLKEARSAIFNGANIVV
                     VNVIDSGFSWVGINSDSEVLKTTKEFLKTIE"
BASE COUNT          933 a          298 c          424 g          780 t
ORIGIN      
        1 ataaaaaaag atatctagct atagagttac ttacttatcc tcaaggcctt gattcaaaaa
       61 caatttatca atctttttca agagctttac aaaagcaaag tggcattttt gttcaattag
      121 agacaaatat tacaatcata gaaatggatt ctaaaacgaa aactatatta attagagtaa
      181 ataaaatata tttagataaa tttgttgcat cgttattttt tgcacaacag gatttaggat
      241 taataaaagt aaaaaacata aaaagcacaa ttaaaaaaat aaatatttaa tgtgttttta
      301 gatatgggga tttaatttat ggacgtaaaa aacacaacgc cagaatcgca aaaatccaat
      361 ctacttatcg cagtaaaagg catatccatc tattatggtt ttgtttcttt attttatctt
      421 attttaggaa ccgccattgt gtttttttct aagaaaataa taacttttct aacatctaat
      481 gtaaaagtag attctgcaaa ttatctaacc gttagtaaat atttaacggt aagtaacgcc
      541 gtcattattg ggggaatttt actgagtatt ggacttttta ttctgttttc tgctattggt
      601 ctgtggaagt taaaaaacag tgcaagaata tatttaatta tagttacaat tttttcgtgt
      661 gtatcctttt ttttctgggg tgttttttct atattttctt tagtttttaa tatattcata
      721 ttaaacatat tatttaaatc taaaaagcta tttatttcaa aagatgattt agaaaccaca
      781 aatgtggatt taaataaaaa agaagtttgg aggtaatatt atgatagggg cagtaataat
      841 tatatttttg gttattggaa tagttgtgtt tttttcaggg ctattacaag tttaccaatt
      901 tcaaagagca gtagtattta ctttaggtaa atttagtcat gttaaagaac caggtcttgg
      961 ctggattgtt ccaattattc aaagagcaag aataatagat atgagaataa aaactgcaga
     1021 tgttccaaga caagaagtta tgacaaaaga taacattcca gttcttgcaa acacggttgt
     1081 ttattttaag gtcgaaaaac cagaagacgt agtaattaag attgaggatt ttgaatacgc
     1141 agtaagacaa tacacccaag cagcattaag agatgttata ggtaatgcag agcttgattt
     1201 tgtgttaagt gaaagagaaa agattgcaaa cagcataaaa gaaattgttg atgcagaaac
     1261 cacagattgg ggggtagaca tagaatctat aaagattcaa gaaatagagt tgccttctga
     1321 aatgaaaaga gcaatggcaa aacaagcaga agcagaaaga gaaagaagag cagtaattat
     1381 tgcagcagat ggagaattaa aagcttctga aaatcttaaa aaagcagcag ataatcttgc
     1441 aacaaatccg gtggcaattc aattaagaac tcttcaaaca ataagagaca ttgccgcaga
     1501 tccttctgaa aaaatagttt tgtttatgcc aacagaaatt gttgaaactg taaaaaagat
     1561 tgtaaaaaaa taaatgggta ataatgtgag gggattgttt tttgaaaatc taaggcgtat
     1621 aagtttagat ttaaatgggc aaacctctaa catctatcag ccacgccgtg ctccaaaaaa
     1681 atttgatact aaaaaaagat atttacaact cgcgtttaat agtagcacgg ctcaagctat
     1741 aaattttatt tctcaactag aatataatga acgaataatt atagaagtag gaactccatt
     1801 aataaaaaaa gaaggcgttt ctgctattag gactattaga aattattggc caggatttat
     1861 tgttgcagac ataaagattg cagatggcgc ggagcaagaa gtagaatatg tttacagaga
     1921 aggcgcagac gcagtaacag tgttaggttg tacaaataaa gaaacaatag actattgcat
     1981 ttctgtatgt aaaaaatata atctaatatc tatggttgat atgattggag tgtctgaacc
     2041 acacagggtt ttattatctt taaaaaaaca accagatgtt ataattcttc ataaagccag
     2101 agatcaagaa cacataaaag gatttaaaat accctataag caagtaaata aaataagaag
     2161 caaatatggc gcattaatat ctgcagctgg cggcgttgat ctaaaagaag caagaagtgc
     2221 aatatttaat ggtgcaaata tagtggttgt taatgttatt gattctgggt tttcatgggt
     2281 tggcataaat agcgattcag aggttcttaa aactacaaaa gagttcctaa aaacaataga
     2341 ataattagtt ctttttttaa taacttaagc tttttaaatg cttcttgtct ataatatata
     2401 ttagataaac tattgtttta tatatttaaa tatga
//
LOCUS       DAOOON010000052         2202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_61210_length_2202_cov_16.982301, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000052 DAOOON010000000
VERSION     DAOOON010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61210_length_2202_cov_16.982301"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..658)
                     /locus_tag="PLK55_04315"
     CDS             complement(71..658)
                     /locus_tag="PLK55_04315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ36177.1"
                     /translation="MNHIKELKKFNNTYYVIRHGQSRANIKKLIMSNPKYAVKNYGLS
                     EVGKQQARDSIKANKDLDKNTIIYSSDFKRARETAEIVRAHIKAKPIILTKSLRERYF
                     GKYDRTADKNYDKVWELDIKDKPLGDIESVYSVADRATRLVLDLEKKYKGKRIVLAAH
                     GDIVNILLTCFNKKDPKYHHTLFSLKTAEIRKANK"
     gene            complement(714..1361)
                     /locus_tag="PLK55_04320"
     CDS             complement(714..1361)
                     /locus_tag="PLK55_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOZ36178.1"
                     /translation="MNKKKIIEKTKEYVKQEMLKGDSGHDWSHVLRVNNTAKMIAKKE
                     KADLFIVELSTLFHDIADSKFTNGDETIAPRKIKDFLNSLKVDTETIEKVNYIVEHMS
                     FKKNQPTEKSLEFKVVQDADRLDAIGAIGIARAFSFGGYKNRLMYNPEEKPNLNQTKE
                     QYVKAEGTTINHFYEKLLLLKDLMNTETAKAIAEKRHKFMEKYLDQFYKEWNGKA"
     gene            complement(1358..>2202)
                     /gene="mnmA"
                     /locus_tag="PLK55_04325"
     CDS             complement(1358..>2202)
                     /gene="mnmA"
                     /locus_tag="PLK55_04325"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOZ36179.1"
                     /translation="LGIEYHVVDERKNFEKEVVGYFKKELKNNRTPNPCVVCNRYSKI
                     KALLDFADKHKIKYVATGHYAKIKNGFLCKPKDKTKDQTYGLCLLKKEWLKRVVFPLE
                     DMTKEQVYKLAQKHDFDYFSKTKQSQDFCYVSGNDISKFIENALGNNPGEIVTTTGEN
                     QGSHNGLWYYTLGQRRGLGRNGTKFYVCGFDTKKNQLIVTSTLENKIKEITLEKVNLL
                     GKLPKKINVKVRYTPNEISATVLLLSNNRAKVIFDKPQAVVTPGQFCVFYSKDKCLGG
                     GIIK"
BASE COUNT          600 a          370 c          292 g          940 t
ORIGIN      
        1 cttattccat agagaatact atatattaag aatcaaaggc tttttaagtg tgtgggctta
       61 gaaaaacaaa ttatttgttt gcttttctta tttctgctgt ttttaaagaa aataatgtgt
      121 gatgatattt tgggtctttc ttattaaaac aggttagaag aatatttaca atatcgccat
      181 gagcggcaag cactattctt ttgcctttat attttttttc taaatctaaa actaatcttg
      241 ttgctctgtc tgcaacagaa taaacacttt ctatgtctcc taatggttta tcttttatat
      301 ctaattccca aactttgtcg taatttttat ctgcagttct atcgtatttt ccaaaatatc
      361 tttctcttaa ggatttggtt aaaattattg gttttgcctt aatgtgtgct ctaacaattt
      421 cagcggtttc tcttgctctt ttaaagtcag aagaataaat tattgtattc ttatctaagt
      481 ctttatttgc tttaattgaa tctcttgcct gttgtttacc cacctctgaa agcccataat
      541 ttttaactgc atactttgga ttactcatta ttaatttctt tatgtttgcg cgactttgtc
      601 catgtcttat gacataatag gtattattga atttctttaa ttcttttata tgattcattt
      661 aattacctct ttaatttttt tatatttata taaccgatat atatatatat atatcaggct
      721 ttaccattcc attccttgta aaattgatct aagtattttt ccataaattt gtgtcttttt
      781 tctgctattg cttttgcagt ttctgtgttc attaagtctt ttaaaagtaa aagcttttca
      841 tagaagtgat taatggttgt gccttctgct ttaacatatt gttcttttgt ttgattaagg
      901 tttggtttct cctctggatt gtacattaat ctgtttttat atcctccaaa agaaaaagct
      961 cttgctatcc ctatggcccc aatggcatct agcctatctg catcttggac tactttaaat
     1021 tctagacttt tttcagttgg ttgatttttc ttaaaagaca tgtgttcaac aatataattt
     1081 actttttcaa tagtttctgt gtcgactttt aaactattca aaaaatcttt tatttttctt
     1141 ggggcaatgg tttcgtctcc gtttgtaaat ttcgagtcag caatatcatg aaacaaagtt
     1201 gaaagttcta caataaaaag atctgctttt tctttttttg caatcatctt tgcagtgttg
     1261 ttaactctta gaacatgcga ccaatcgtgt cctgaatctc ctttaagcat ctcttgttta
     1321 acatattctt ttgttttttc aataatcttt tttttattca ttttattatt cccccgccta
     1381 aacatttatc cttagaataa aatacacaaa attgtccagg agtaacaact gcttgtggct
     1441 tatcaaaaat tacttttgct ctgttatttg aaagtaacaa aactgttgca gaaatctcat
     1501 ttggggtgta tctaactttt acgtttattt tttttggaag tttacctaaa agatttactt
     1561 tctcaagagt tatttcttta attttatttt caagagtgct tgtaactatt aattgatttt
     1621 tcttagtatc aaatccacaa acataaaact ttgtgccgtt tctcccaaga ccccttcttt
     1681 gaccaagagt gtaataccaa agaccgttgt gacttccttg gttttctcct gttgttgtta
     1741 ctatctctcc tgggttattt ccaagtgcat tttcaataaa cttagaaata tcatttccag
     1801 aaacataaca aaaatcttgg ctttgtttag ttttggagaa ataatcaaaa tcgtgctttt
     1861 gagctaactt gtaaacttgt tctttagtca tatcttctaa tgggaaaaca actcttttta
     1921 gccactcttt ttttagaaga caaaggccgt aagtctgatc ttttgtttta tcttttggtt
     1981 tacacaaaaa accgtttttt atttttgcat aatgtcctgt agcaacatac tttatcttat
     2041 gtttgtctgc aaaatctaaa agtgctttaa tctttgaata tctgttgcac acaacacaag
     2101 gatttggagt tctgttgttc tttagttctt tcttaaaata ccctacaact tctttttcaa
     2161 agttttttct ttcatcaacc acgtgatatt caattccgag ct
//
LOCUS       DAOOON010000053         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_61512_length_2195_cov_14.273364, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000053 DAOOON010000000
VERSION     DAOOON010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61512_length_2195_cov_14.273364"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2195
                     /locus_tag="PLK55_04330"
     CDS             <1..>2195
                     /locus_tag="PLK55_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36180.1"
                     /translation="AEGSNFLGSAISQVGGVLTNSIGGTLTGIISTVTGNPVITGVVG
                     AGVSAYLTYDALSDLNVSKLMHTDRVNISDRVPDFFNEDSKKELVIDPAKEIVLTDKN
                     VLDVDFVVTVKDLTTYRDLTKNPNMRLTEKTEEIKVLDSNNQPVAGQKRGVLFEDANQ
                     TIKQAYEYRTLLIKYKEAKYDKQPVKLEIGASDIVNIGWLFNFYSSGAESAKSEIDEK
                     IFSFDITKNKHPEIPVEKIQKFHLLFNSRVDENAEPIDSSLLDCTTEDGKILGTTGTE
                     VLPRVVYNWDFKENNIVTPITGETTKVKNSEWCDVSNNGVYCDATQLSIEVLQKINKI
                     NEIVSASNASFTCPTAVTDQALISDINNVGITYINVENISSSRLKFTYKIRGNYQLPS
                     TFSASGSLFDINYEVTKKADNSFNWVKIASGAIPVDQSYLNTGGYLEEEIEIDEFLEG
                     PIDTDIIEIKLTLTNFHESIANNETGNNLLDNTLTTQTNIAGGTIGCSLPKTSENLNK
                     YYSGFDEKLYHFKAYLMRDGYSDDFKKDFDEYYRLTFLKDTPHWYGSSDILTTVSNYM
                     PLYKYFTDIEKFKFVLMNSGSVTNQTLSGPGRYDIKIIIHYDDSWRLFNDAGNMTGKI
                     EIMISKEQNPEIDYPLYYMPLDGLVGSTGDKTRNGYGVDYLNDIVPIYLDSQNNYSGT
                     MFTEGYVSSNTVSTIDTKEVKEFAIMNNGNERGKLLSINREQNNLLKLR"
BASE COUNT          940 a          303 c          358 g          594 t
ORIGIN      
        1 gccgaaggtt ctaacttctt aggaagcgca attagccaag ttggcggagt attaactaat
       61 tcaattggag gcacacttac tggaataata agtacagtaa ctggtaatcc cgtaattaca
      121 ggagttgttg gcgcaggagt ttcagcatat ctgacttatg atgcattaag cgatctaaat
      181 gttagtaaat taatgcacac agatagagta aatatctctg atagagtgcc tgatttcttt
      241 aacgaagatt caaaaaaaga actagtaatt gatccagcta aagaaatagt attaactgat
      301 aaaaatgttt tagatgttga ttttgtagta acagtaaaag atttaacaac ctatagagat
      361 ttaactaaaa atccaaatat gagactaaca gaaaaaaccg aagaaataaa agtactagat
      421 tctaataacc aaccagttgc aggtcaaaaa agaggagtat tattcgaaga tgcaaatcaa
      481 acaattaaac aagcctacga gtatagaaca ctattaataa aatataaaga agcaaaatat
      541 gataaacaac cagtaaagct cgaaatcgga gcaagcgaca tagtaaatat aggctggtta
      601 tttaatttct atagctcagg agcagaatct gcaaaatcag aaattgatga aaaaatattt
      661 tcttttgata taactaaaaa caaacatcca gaaataccag tagaaaaaat acaaaaattc
      721 catttattat ttaattctag agttgatgaa aacgcagaac caatagattc aagtctatta
      781 gactgtacaa cagaagatgg aaagattcta gggacaacag gaacagaagt tctaccaaga
      841 gtagtataca attgggattt taaagaaaat aacatagtaa caccaataac tggagaaacc
      901 acaaaagtaa aaaacagcga atggtgtgat gtttctaata atggtgtata ttgtgatgca
      961 actcaactat caatagaagt cttacaaaaa ataaataaaa taaacgaaat tgtaagtgca
     1021 agtaatgcat catttacttg tccaacagca gttacagatc aagcactaat aagcgatatc
     1081 aataatgtag gaataaccta tataaatgta gaaaacataa gtagtagcag attaaaattt
     1141 acttataaaa ttagagggaa ctatcaattg ccttctacat tttcagcttc aggatcttta
     1201 tttgatataa attatgaagt tacaaagaaa gcagataact cctttaattg ggtaaagatt
     1261 gcaagcgggg caatacctgt agaccaaagc tatctaaata caggaggata tctagaagaa
     1321 gaaatagaaa tagacgaatt tttagaaggc ccaatagata cagacataat agaaataaaa
     1381 ctaacattaa caaatttcca cgaatcaata gcaaataatg aaacaggaaa taatttacta
     1441 gataatactt taacaacaca aacaaatata gcaggtggca caataggttg tagtctacct
     1501 aaaacttcag aaaatttaaa taaatactat tctggctttg atgaaaaatt atatcacttt
     1561 aaagcatact taatgagaga cggatatagt gatgatttca aaaaagactt cgatgaatat
     1621 tatagattaa ctttcttaaa agacacacca cactggtatg ggtcatcaga tatcctaacc
     1681 actgtttcaa attatatgcc actatataaa tattttacag atatagaaaa attcaagttt
     1741 gttttaatga attctggttc agtaacaaac caaacattat caggaccagg aagatacgac
     1801 ataaaaataa taatacatta tgatgatagt tggagattat tcaacgatgc aggaaacatg
     1861 acaggaaaaa tagaaataat gataagtaaa gaacaaaatc cagaaataga ttatccacta
     1921 tactatatgc ctcttgatgg attagtagga agtactggag acaaaacaag aaacggttat
     1981 ggtgtagatt atctaaacga tatcgttcca atttatctag attcacaaaa taattattct
     2041 ggaactatgt ttactgaagg ttatgtctct tcaaacacag tttcaacaat agatacaaaa
     2101 gaagtaaaag aatttgcaat aatgaataat ggaaatgaaa gaggtaagct attatctatt
     2161 aatagagaac agaataatct attaaaacta agata
//
LOCUS       DAOOON010000054         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_61630_length_2192_cov_12.057557, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000054 DAOOON010000000
VERSION     DAOOON010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61630_length_2192_cov_12.057557"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLK55_04335"
     CDS             <1..425
                     /locus_tag="PLK55_04335"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOZ36181.1"
                     /translation="IQVMTFVSVLFGIVSIASDIALRGMIFGGGDHDSNNKNPIGLIL
                     AIALVILAPLIALIIQMALSRNREYLADSSGAKLTRYPAGLANALKKIAAVNQPVKKA
                     SKGTAHLYIANPLSGGGFTNIFSTHPPIQERIKRLEAM"
     gene            434..1858
                     /locus_tag="PLK55_04340"
     CDS             434..1858
                     /locus_tag="PLK55_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HOZ36182.1"
                     /translation="MKTKQKAITPIVATILLVVVSVVLLTIVLNWGRDFSNKSLATVN
                     TEKLSKSNASEFVYTKAFSDGVIQFNYAPPANLKDQDIIITGYKIFFDNNETTEISFT
                     SNHTLSQGLNIIPLTSFSDQNITSRKMDIQLQTSDGKYITLNSVTNNEPYVAEPYVPQ
                     QYTAGPLSPGTMADDSAIGTEAWSNVDNSKTSDNIYSGAFIVLLDFYDFGYTTHYLKA
                     TNFGFNIPIGATINGIKVEVEEYGSGPLDSSIAKIVKSNGSIGTTSIFDYNSPSSDMN
                     IIFGDSNNLWGETWTAEQINDSNFGVAIYIIGHTSGQCLNENSLISTDNGYIKIKDLQ
                     IGDDVVSYNDTTKQLELKPITSVWSNPISIANNRYFYIYYNNGEVIKATENHRFYVSG
                     EYKRADELLIGDLLLSQDFQNYAIENIEIIENTTDKVWDIEVEDNHNFFANDILVHNP
                     LPAPSPEEFYAYVDHVRMTVYYTN"
     gene            1950..2084
                     /locus_tag="PLK55_04345"
     CDS             1950..2084
                     /locus_tag="PLK55_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36183.1"
                     /translation="MKEWTIEITQKMKAAMDRYPKVDWNKVAEEGIKKEIQKIESKKK
                     "
BASE COUNT          810 a          332 c          357 g          693 t
ORIGIN      
        1 acatacaagt tatgactttt gtgtctgttc tttttggtat tgtttcaatt gcttcagata
       61 ttgcattaag aggaatgata tttggcggcg gagatcatga tagtaataat aaaaatccaa
      121 taggtttgat tctagccatt gctttagtaa ttcttgctcc attaattgct ttaattattc
      181 aaatggcatt atctagaaac agagaatatt tagcagactc ttctggagca aaattaacta
      241 gatatcctgc aggtcttgca aatgctctta aaaaaatagc tgcggtcaat caaccagtaa
      301 agaaagcaag taaaggcaca gctcatcttt atattgctaa tcctctttcc ggcggtggtt
      361 tcacaaacat cttttcaacc cacccaccaa tacaagaaag aatcaagaga ctagaagcaa
      421 tgtaattttt attatgaaaa caaaacaaaa agcaatcacc ccaattgttg caacaattct
      481 tttagtagta gtttcagtag tacttttaac tatagttcta aactggggaa gagactttag
      541 caataaatcc ttagcaacag taaacacaga aaagctatct aaatctaatg cttcagagtt
      601 tgtttatacc aaggcatttt cagatggtgt tattcaattc aactacgctc cgccagccaa
      661 tcttaaagac caagatatta taatcacggg ctacaagata ttttttgata acaacgaaac
      721 cacagaaatt agttttacat ctaatcacac actttcccaa ggtttaaaca taattccatt
      781 aacatctttt tcagaccaaa atattacttc aagaaaaatg gatatacaac tacaaacatc
      841 tgatggaaaa tatataactt tgaatagcgt tactaataat gaaccttatg ttgctgagcc
      901 atatgttcca caacaatata ctgcggggcc attaagtcca ggaacaatgg cagatgattc
      961 ggctattgga acagaggcgt ggagtaatgt agacaattct aagacaagcg ataatattta
     1021 ttctggtgca tttattgttt tattagattt ctatgacttc ggatatacta cacattatct
     1081 taaagcaact aattttggtt ttaatatccc aataggggcg acaataaatg gaattaaagt
     1141 tgaagttgaa gaatatggtt ctggaccatt agatagttca atagcaaaaa ttgttaaatc
     1201 aaatgggtca attggaacaa ctagtatttt tgattataac tctccaagtt cagatatgaa
     1261 tataattttt ggtgatagta ataatttgtg gggagaaacc tggactgcag agcaaataaa
     1321 tgattcaaac tttggagttg caatttatat tattggtcat acttccgggc agtgtttaaa
     1381 tgaaaattca ttaatttcaa cagacaatgg ttatataaaa ataaaagact tacaaattgg
     1441 agacgatgtt gtttcatata atgataccac taaacaatta gaattaaaac caataacaag
     1501 tgtttggtca aatccaattt caatagcaaa taatagatac ttctatattt attataataa
     1561 tggagaagtt ataaaagcca cagaaaacca tagattttat gtttctggag aatataaaag
     1621 agcagatgaa ttattaatag gtgatttatt attatcgcaa gattttcaaa actatgctat
     1681 tgaaaatata gaaattatag aaaatactac tgataaagta tgggatattg aagtagaaga
     1741 taatcataat ttctttgcta atgatatttt agttcacaat ccgctacccg caccatcgcc
     1801 agaggagttt tatgcatatg tggatcacgt tcgtatgacg gtgtattata caaattaatg
     1861 ctctgtcatt ttcttttttc cgcattggac aactaccctc aaaagctttt taaactacta
     1921 attctattaa taatatattt ggtgataata tgaaagaatg gactattgaa attactcaga
     1981 agatgaaagc cgcaatggac agatatccaa aagttgattg gaataaagtc gcagaagagg
     2041 gtattaaaaa agaaatacaa aaaatagaat ctaagaagaa ataaacatgg aacaagatat
     2101 caataacatt gtagagcaga tgcttaatgg gtataataag aaccaagtct ttatttctag
     2161 gcttcgttct cttaatattt ctgaaattac ta
//
LOCUS       DAOOON010000055         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_69246_length_2027_cov_18.519777, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000055 DAOOON010000000
VERSION     DAOOON010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69246_length_2027_cov_18.519777"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..355
                     /locus_tag="PLK55_04350"
     CDS             74..355
                     /locus_tag="PLK55_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36184.1"
                     /translation="MSKKIKITDFIPGVLAILLFVARYIKFYKVVDFGDLGKVYISFV
                     QKMQECSKPLIGMLDTCSGIGTTNTIWWVALVLLLALQGFILYKKFAKK"
     gene            complement(379..1017)
                     /locus_tag="PLK55_04355"
     CDS             complement(379..1017)
                     /locus_tag="PLK55_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36185.1"
                     /translation="MMTKEELLLELKKRPIPQELLETKEKAIQEFNDFFGIRLQKQFN
                     IYIIYTREEMDIIQEKKTADWVAGHTRDYGTKIFIFDPEYYEKETGKKQIYFKKLLKH
                     EIVHVYIREIINGKCPRWLNEGLAGYLPKQNTTDKPIEEIVNCIDCFDDFVFEHYRTS
                     SKLIELLITKYGKEKLLAILESLKNKEKFISAFQNIYGFEFTKENLIENLKE"
     gene            1099..1929
                     /locus_tag="PLK55_04360"
     CDS             1099..1929
                     /locus_tag="PLK55_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36186.1"
                     /translation="MVVEINLKLIKPSKELRLACFGYDNIGKTRKYGPRAGLDGYLKL
                     FSCINKDWELILGPDAHYRELIDKVILKKLNSKDKQLLKSLKKIVKIKPYPKVERLLN
                     KELIKYKEKIFPKIISEFDKIYTPTKAKLVVFSVSSRTDYGGSATEHSYYLSADNLER
                     VKKCSKDNDNYHLITHELIHAINSNNSIYKNIEKRISSDKRSNNHSDFSEVFTETITN
                     IVCYNAGLEKAKWRSYRYPESKKACLFEDELRKHYETWEKQKTKNKPKFIEYLDKKII
                     "
BASE COUNT          735 a          270 c          273 g          749 t
ORIGIN      
        1 tgtcttaaag attataaggc ccaaaaacaa acaccataaa attacactta tttaatacgg
       61 aggcaatgat ataatgtcaa agaaaataaa aataacagat ttcatacctg gggttcttgc
      121 aattttatta tttgttgcaa gatatataaa attttacaaa gttgtagact ttggagattt
      181 aggaaaagta tatatctctt ttgtacaaaa gatgcaagaa tgtagtaagc cactaattgg
      241 aatgttagat acttgttcag gaataggaac aacaaacaca atttggtggg tagcactagt
      301 tttgttatta gcattacaag gctttatact ttacaagaaa tttgctaaaa agtaattttt
      361 ttgtaatttc tttttctttt attcttttaa attttcaatt aaattttctt ttgtgaattc
      421 aaagccatat atattttgaa aagcagaaat aaatttttct ttatttttta aagattcaag
      481 aatagcaagt agtttttctt ttccatattt agtaattaat aattctatta atttgcttga
      541 tgtcctataa tgttcaaata caaaatcatc aaagcaatca atacaattaa ctatttcttc
      601 tataggttta tcggtagtat tctgttttgg taaatatcca gcaagtcctt cgtttaacca
      661 tctagggcat ttgccgttta ttatttctct aatataaaca tgcacaattt catgcttaag
      721 taatttttta aaatagattt gttttttacc tgtttctttt tcatagtatt ctggatcaaa
      781 gataaatatt tttgtcccat aatcccttgt gtgtcctgca acccaatctg cagttttctt
      841 ttcttgaata atatccattt cttctctagt gtagattatg taaatgttaa attgtttttg
      901 taacctgatt ccaaaaaaat cattaaactc ttgtattgct ttttccttag tttctaaaag
      961 ttcttgagga ataggccttt ttttcaattc taaaagtagt tcttcttttg tcatcataat
     1021 agttactagg tatcaaaagc attttaagct tggttatcta aaaatcatat tttaaattgt
     1081 ttgttatact atatatgtat ggttgttgaa ataaacctaa aactaattaa accaagtaaa
     1141 gagctaagat tggcttgctt tggctatgat aacattggta agactcgaaa gtatggccct
     1201 agagctggat tagacggtta tctaaaacta ttttcttgta tcaataaaga ctgggaacta
     1261 attcttggtc ctgatgctca ttatagagaa ctaattgata aagtcatatt aaagaaacta
     1321 aattctaaag ataaacaatt attaaaatct ttaaaaaaga tagtaaaaat aaaaccatat
     1381 ccaaaagttg aaaggcttct aaataaagaa cttatcaaat ataaggaaaa aatttttcct
     1441 aagattattt ctgagtttga taaaatatat actccaacta aagcaaagtt agttgttttt
     1501 tcagtaagtt caagaacaga ctatggtggt tcagcaactg agcattcgta ttatctttct
     1561 gcagataatc ttgaaagagt taaaaagtgt tcaaaagata atgataatta tcatcttata
     1621 actcatgagc ttattcatgc aattaattct aataattcaa tatataagaa tatagaaaaa
     1681 agaataagtt ctgataaaag atcaaacaac cattctgatt tttctgaagt ctttacagaa
     1741 accataacca atattgtctg ttataatgca gggttagaga aagcaaagtg gcgtagttat
     1801 agataccctg aaagtaagaa agcctgtctc tttgaagatg aactaagaaa acactatgaa
     1861 acttgggaaa aacaaaagac aaaaaacaag ccaaagttta tagagtattt agataaaaaa
     1921 ataatttaaa aatctaaaat ttcttcttct ttaaagaaac ctttattctt tattttaatt
     1981 cctttttctt ttgtaacttt accaattact tttccttcaa tgttatg
//
LOCUS       DAOOON010000056         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_70067_length_2012_cov_10.037302, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000056 DAOOON010000000
VERSION     DAOOON010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70067_length_2012_cov_10.037302"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1031
                     /locus_tag="PLK55_04365"
     CDS             324..1031
                     /locus_tag="PLK55_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36187.1"
                     /translation="MAIDYKKLDKTILLFCPSIIILMIIVGILSSTMGAPISRFPIIS
                     AIFIFLGTFSAVYLIWSFCFWIIGLISKKTNYKFNFIIKSATSIVLAILIVYILSIPL
                     CGDNCGGRNTSPTTNAKTLLGTQVNNPGAESCTESVVFTKQNSQLSAEGITQNTGLDP
                     EQVIFSNPQDISNFQASNSLLKYTGTANKRVIMCIVCSEGKTNLERALIDNEINVTVN
                     STIEGQTLCIVYPRKTA"
     gene            1157..>2012
                     /locus_tag="PLK55_04370"
     CDS             1157..>2012
                     /locus_tag="PLK55_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOZ36188.1"
                     /translation="MGMDYENIRWLKELGSTDSRTIGRKAARLSQLATSGIAVPRGFV
                     VTATEFFNFIQTNNLKSKIVEILRSINYNDRQDIENKSEQIKQILLKGKLNQEAEIEL
                     YKYYGKLGDVGGGWLPSSADAFVAVRCSVVCDDGEVCDTKFIEEEGGSLNIKGKAELV
                     KCLKETWGILYSPDVLEYAHRKGINVERLGIGVIIQNMITAKYSGITISSKSSTDPNT
                     VIIEAVYGFGAAHVIKEITPDHYETQKKPYVTLGKTISRQDWELKRIKGQTVRADVPE
                     SEREKQKVN"
BASE COUNT          738 a          296 c          351 g          627 t
ORIGIN      
        1 ttctaaattt tcttgatttt caaattctaa actcatggtt tcttccttct tttctctctt
       61 cataaatatc tactcctttt gtttttttaa acccttttat ttgggtctaa taatatatgg
      121 gcattcaaaa gggggttgtt ttcggggggg taggtgtcca gtgcggaatc ggtttcgcgc
      181 gattcgtgcg cagtggcttt cgcggcttat ccgcgggttt tttagaggct acaaaatttc
      241 tgctaaaata cccattttta cctaagaaaa caaataacct tttaaaaggt tagatattat
      301 cattaatatt aattggtgaa attatggcta tagattataa aaaactagac aaaacaattc
      361 tgttattttg cccttctata attattttaa tgatcatagt aggaatattg tcttcaacaa
      421 tgggggcgcc tatatctcga ttcccaataa tttccgcaat ttttattttt ttgggaacat
      481 tttctgcagt atatttaatt tggtcatttt gtttttggat tattggatta atatcaaaga
      541 aaaccaacta caaatttaat tttataataa aatctgctac aagtattgtt ttagcaatat
      601 taattgttta tatattatca ataccattat gcggagataa ttgtggcgga agaaacacca
      661 gtccgacaac taatgcaaaa acattattgg gaacgcaagt aaataatcct ggtgctgaaa
      721 gttgtactga atcagtagtc tttacaaaac aaaattctca attatctgca gaagggatta
      781 cacaaaatac tggtctagat ccagagcaag ttattttttc aaacccacaa gatatttcta
      841 attttcaagc aagtaattct ttattaaaat atactggaac cgcaaataag agagtaataa
      901 tgtgtattgt ttgttctgaa ggaaaaacta acttagagcg agcattaatc gataatgaaa
      961 ttaacgtaac agttaattca acaatagaag gacaaacact ttgtatagtt tatcctagaa
     1021 aaactgcata aaataaggaa tcttataact gcttattcta agcaaatatc ttctgtggat
     1081 tagttttttt aaccaaaaag cctttaaaaa gaatctatat tatattattg agattaaata
     1141 aaaaatgtga aataacatgg gaatggacta tgaaaacata cgttggctta aagaactagg
     1201 ttcaacagat tctagaacaa ttggaagaaa agcagcaaga ctttcgcagc tagcaacaag
     1261 cgggattgca gttccaagag gttttgttgt tacagcaaca gaattcttta attttataca
     1321 aactaataat ttaaaatcaa aaatcgtaga gattctaaga agcataaact ataatgatag
     1381 acaagacata gaaaataaat cagaacaaat aaaacaaatt ctattaaaag gaaaactaaa
     1441 ccaagaagcc gaaatcgaac tatacaaata ttatgggaaa ttaggagatg ttggtggcgg
     1501 ttggctacca agtagcgcag atgcttttgt tgctgttagg tgctctgttg tttgcgatga
     1561 cggagaggtt tgcgatacta agtttattga agaagaaggc ggctccttaa atattaaagg
     1621 aaaagcagaa cttgtaaagt gcttaaaaga aacctggggc atactttata gcccagatgt
     1681 tttagaatat gcacacagaa aaggaataaa tgttgaaaga cttggtatcg gtgtaattat
     1741 tcaaaacatg attactgcta aatattcagg aataacaatc tccagcaaaa gctcaacaga
     1801 tccaaacaca gttattattg aagcagttta tggctttggt gcagctcacg ttattaaaga
     1861 aataactcct gaccattatg aaacacagaa aaaaccatat gtaacacttg gtaaaactat
     1921 ttctagacaa gactgggaac taaaaagaat aaagggacaa actgttagag ctgacgtgcc
     1981 tgaatcagaa agagagaaac aaaaagttaa ta
//
LOCUS       DAOOON010000057         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_71291_length_1987_cov_7.390787, whole genome shotgun sequence.
ACCESSION   DAOOON010000057 DAOOON010000000
VERSION     DAOOON010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71291_length_1987_cov_7.390787"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..795
                     /locus_tag="PLK55_04375"
     CDS             424..795
                     /locus_tag="PLK55_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36189.1"
                     /translation="MKSKKIISVYYSREFNEEYLAIKAVVDMEVKKGLLNSQNHQLLT
                     KIDRTIEILKSDPDYGVSIAKDIIPKELIKEYNINNLRKVNLSNYWRLCYTIKTDSVE
                     IVSIVLKVMDHKKYNKLFGYK"
     gene            complement(797..1078)
                     /locus_tag="PLK55_04380"
     CDS             complement(797..1078)
                     /locus_tag="PLK55_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36190.1"
                     /translation="MNLEKNIQKGVLHYPTLKTILAIEKTIKEANKPINREQIKQKLQ
                     TKIHHQTLNLVLSYLEESGKIFIGKDGITWTFNDSLKFKKLVKEAIKVK"
     gene            1153..1914
                     /locus_tag="PLK55_04385"
     CDS             1153..1914
                     /locus_tag="PLK55_04385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOZ36191.1"
                     /translation="MSKNTNVETNTKKMYNDFGERYQERRYYNDYVEIPNMLKTVKNI
                     KGKRLLDIGCGAGVHIKDYSKRGAKCSGIDISETMIEMAKKNNPKVEFKVGTVTKLPY
                     KSNSFDVVTCSLILDYIADLNLAYKEINRILKKGGILYYSYNSPVACVREDYEDNNYK
                     IRGLGKFIDKKRNKTIFLGQNGRLEHVEWIPGMKMICYWYPINVHLKGIVKNNLELID
                     LVDCYPIKSFKKLYPEKYKHHTKYPLWTIYVCRKK"
BASE COUNT          688 a          226 c          338 g          735 t
ORIGIN      
        1 gtgtttctgt aatgtaagga ttagacttta cactataagc tacaatatct gttagatctt
       61 gtaatttaga ataattctct atgattgcct ttctagaaaa gacaaactta gctttagaaa
      121 acataaatat aatatggctc aaaaggtttt aaaatagtag tttgatttaa actttcctat
      181 ttctcgcgcc aatggcgcga gttttagatt aatttcttta gttcttattt ttagtccact
      241 tgaattcgaa ggaattagat taattagtca ggcactgcct gaccttttta gtaattttga
      301 attatccagg cactgcctgg ataattggat atttgtagat tctctaaatt atgtccaata
      361 ttttgtgttt ttacaaagaa tctaatagaa gaaaatataa acctctaatt gtctaagaaa
      421 tatatgaaat caaaaaagat aatttctgtt tattactcta gagagtttaa tgaagagtat
      481 ctagcaatta aggcagttgt tgatatggaa gtaaagaaag gacttctaaa tagccaaaac
      541 catcaactgc ttacaaaaat tgatagaact attgagattc taaaatctga tcctgattat
      601 ggtgtttcta ttgctaaaga tattatccct aaggagttaa ttaaagaata caatataaac
      661 aatctaagga aagtaaatct ttctaattat tggcgtcttt gttatactat taagacagat
      721 agtgtagaga ttgtttctat tgtgcttaag gttatggatc ataagaagta taataagttg
      781 tttgggtata agtaatttat tttactttta ttgcttcttt tactagtttt ttaaacttta
      841 agctgtcatt aaaggtccag gttattccat cttttcctat aaatatcttg ccgctttctt
      901 ctaggtagga taatacaagg ttaagtgttt ggtggtgtat ctttgtttgt agtttttgtt
      961 tgatttgttc tctattgatt ggtttgtttg cttcttttat tgttttttca atagcaagta
     1021 ttgtttttag ggttgggtag tgtagtactc ccttttgtat gtttttttct aagttcatat
     1081 atacatatat gtatatgtat aagtttatat aaaccttttg attttaaaaa ctcttttgtt
     1141 tctaggttat ttatgtctaa aaatacaaat gtagaaacaa ataccaaaaa aatgtataat
     1201 gattttggag aaagatatca agagagaaga tattacaacg attatgttga aattcctaat
     1261 atgttaaaga ctgttaaaaa cataaagggg aagagacttc ttgatattgg ttgtggcgct
     1321 ggtgtgcata ttaaagatta ttctaagaga ggagctaagt gctcaggcat agacattagc
     1381 gaaactatga tagaaatggc taagaagaat aatccaaaag tagagtttaa agtaggaaca
     1441 gttactaagc ttccatataa atctaattcc tttgatgttg ttacttgttc tcttattctt
     1501 gattatattg cggatctaaa tcttgcatat aaagaaataa atagaatctt aaagaaaggc
     1561 ggaatacttt attattctta caatagtcct gttgcttgtg ttagagaaga ctatgaggat
     1621 aataattata agattagagg tcttgggaaa ttcatagaca aaaaaagaaa caaaactatc
     1681 tttcttggtc aaaatggaag attagagcat gttgaatgga ttcctggaat gaaaatgata
     1741 tgttattggt atccaattaa tgttcatcta aaaggaattg tgaaaaataa tttagagtta
     1801 atagatcttg tagattgtta tccaattaag agttttaaaa aactttatcc tgaaaaatat
     1861 aaacatcaca caaagtatcc tctttggaca atttatgttt gtaggaagaa gtaatatggc
     1921 aaattataaa tcaacaacaa aggaaggaaa attgttaaaa taaggattta taccattttt
     1981 cgtattg
//
LOCUS       DAOOON010000058         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_80068_length_1843_cov_20.588926, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000058 DAOOON010000000
VERSION     DAOOON010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80068_length_1843_cov_20.588926"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..698)
                     /locus_tag="PLK55_04390"
     CDS             complement(198..698)
                     /locus_tag="PLK55_04390"
                     /inference="COORDINATES: protein motif:HMM:NF003085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOZ36192.1"
                     /translation="MTFNKPATGGHKPVYFNKQQRTAEQDPNAFRKIKTPNRKELEMY
                     GKVIQLMGSNQVKIACEDSQDRICRIPGKLFKHVWLKANDIVIVKLWDFQPSKADVTW
                     RYLGFQKNALEKRGELTKLYEYTHDEVSNYQEYPTNQKPFVEEKPAEAEEFLDDEENV
                     EDEDNE"
     gene            complement(695..1024)
                     /locus_tag="PLK55_04395"
     CDS             complement(695..1024)
                     /locus_tag="PLK55_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36193.1"
                     /translation="MKKRQAIRKSIKKKIAEKKIAPTFYITKEYSERKLKIMGSVLEA
                     FEKSTFTRKPEIKNIEQGLKKLENHKKQIADSILSRDAKKALIDFCEEWIKKCNSYIE
                     RNKGVKK"
     gene            complement(1035..1325)
                     /locus_tag="PLK55_04400"
     CDS             complement(1035..1325)
                     /locus_tag="PLK55_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HOZ36194.1"
                     /translation="MIGKVHNQNGRTIFACCDKELIDKKLNHGDIEIHLSRSFYGSSL
                     LTEKEFLEQFSDCDYANFFGNRACNLLLKKKIITKDQILYIDKIAHVQIYKM"
     gene            complement(1346..1801)
                     /locus_tag="PLK55_04405"
     CDS             complement(1346..1801)
                     /locus_tag="PLK55_04405"
                     /inference="COORDINATES: protein motif:HMM:NF003067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HOZ36195.1"
                     /translation="MAKTKKEIEKATTDIDLDYNKLLDRLYMSLPSKAKEHTRFELPK
                     AQSIIQGKQTIWKNFVKVSKDLNRDTDVLYKFVMKEISTSSTIVNNSALVLNGTFDYN
                     KINQILDKFINIYVLCSACKKPDTEIVTKNGIKVLRCTACGAVSPLPKL"
BASE COUNT          518 a          313 c          241 g          771 t
ORIGIN      
        1 tttgcaacaa gcggcgtttc tcctaaatgc cccagaaaag taattgcttc tttccctgaa
       61 gctattggag actctataga atcaataata ttgttacttg ataatttaaa taaagactcg
      121 tgtgtttgat tatcaaaaac gtaactttct attttttttg tatcaatcat attaaatcta
      181 aattaaaaaa agaaaattta ttcgttatct tcatcttcaa cgttttcttc gtcatctaaa
      241 aattcttcgg cttctgcagg cttttcttca acaaagggtt tttggttggt tggatattct
      301 tgataattgc ttacttcgtc gtgtgtatat tcataaagtt ttgttagttc tcctcttttc
      361 tctagtgcgt ttttttgaaa acccaaatat ctccaggtta cgtctgcttt acttggttga
      421 aagtcccaaa gcttaacaat tactatgtcg tttgccttta accaaacgtg cttgaaaagc
      481 ttccctggaa ttctgcaaat tctgtcttga gaatcctcac aagcaatctt tacttgatta
      541 gagcccatta attgtattac ttttccatac atctccaatt cttttctatt aggcgttttt
      601 attttcctaa aagcatttgg gtcttgctct gcggttcttt gttgtttatt aaaataaaca
      661 ggcttgtgtc cacctgttgc tggtttatta aatgtcattt cttcactcct ttatttcttt
      721 caatataact attacatttt tttatccatt cttcacaaaa atcaataagt gctttttttg
      781 catctctaga taaaattgag tccgctattt gttttttgtg attttctaat ttttttagtc
      841 cttgttctat attcttaatt tcaggttttc ttgtaaatgt acttttttcg aatgcttcaa
      901 gaactgagcc cattatctta agtttgcgct ctgaatattc tttagttata taaaatgtgg
      961 gcgcaatttt tttctctgca atcttttttt ttattgactt tcttattgct tgtctttttt
     1021 tcataaattt cctcttacat cttatagatt tgcacatgcg caatcttgtc tatatataat
     1081 atttgatcct ttgttattat ttttttcttt aacaatagat tacaagcacg attgccaaaa
     1141 aaatttgcat agtcacaatc agaaaactgc tctaaaaatt ctttttctgt taaaagacta
     1201 gaaccataaa aagacctaga aagatgaatt tcaatgtctc catgattaag ttttttatca
     1261 attaattcct tatcacaaca cgcaaatatt gttctgccat tctgattatg aactttgcct
     1321 atcatcttta tttatttact ccattctata gttttggaag agggctaaca gcgccacaag
     1381 cagtacatct taaaactttt attccatttt tggttactat ctctgtgtct ggttttttac
     1441 aagcagaaca caagacatag atgtttatga acttatcaag tatttgattt atcttattat
     1501 aatcaaaagt tccatttaaa actaatgcag aattattaac aatggttgaa gaagtactta
     1561 tttctttcat aacaaactta tatagaacat ctgtatctct atttaggtct ttagaaacct
     1621 tcacaaaatt cttccagatg gtctgcttac cttgaattat agattgtgct tttggcaatt
     1681 caaatctagt gtgttctttt gcttttgaag gtagagacat atatagtcta tcaagtaatt
     1741 tattgtaatc taaatcaata tcggttgttg ctttttctat ttcttttttt gtctttgcca
     1801 tatatataat atagcataaa tgcttttata aagctattta gag
//
LOCUS       DAOOON010000059         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_82375_length_1811_cov_21.000569, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000059 DAOOON010000000
VERSION     DAOOON010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82375_length_1811_cov_21.000569"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLK55_04410"
     CDS             complement(<1..463)
                     /locus_tag="PLK55_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOZ36196.1"
                     /translation="MENYSKKVMENFMNPKNMGEIKNADGVGKVGNPKCLVESTLLQT
                     NSKLIKIKDIKTKDLIVSHNGKYNKISGIFDRSVNESIIVLKNRLGEIKLTKEHLVYA
                     IKVPKRDKYLRTKNKKTLIPAWYHAEQLDKGDIILYSILSETKDIDYLDVNT"
     gene            complement(466..1626)
                     /locus_tag="PLK55_04415"
     CDS             complement(466..1626)
                     /locus_tag="PLK55_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOZ36197.1"
                     /translation="MKSIYLDNAATTRMHSSVKKAVVEAEEKYFGNSSSLHSVGREAK
                     EKLDIAREKISKKVNSKNHKLIFTSGGTESNNFALKGLAESNKQKKHIITTKIEHDSV
                     LNVTKFLENKGYTITYLDVDKEGFVKIDVLEKALRKETLLVSVIHGNNEIGTLQNIKE
                     ISKIVHDNGSLLHVDACQSFTKYPIDLEKENIDLLTINAHKIYGPKGIGGLFVREGIK
                     LEPLLHGGGHEYKLRSGTVNVPGIIGFSKATDVIGEKEIAHMTTLRNQLVNGLKDVND
                     SWLNGPSINSNKRLCNNANISFMYAEGEAILLYLDALGISVSTGSACSSNTLEPSHVL
                     KAIGLKQEQTHGTVRFSVGIDNTKAEIKFVVDSVKDTVNKLRKMNPYKYKVK"
     gene            complement(1623..>1811)
                     /locus_tag="PLK55_04420"
     CDS             complement(1623..>1811)
                     /locus_tag="PLK55_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36198.1"
                     /translation="EQSKVSHALKILLDCNIVFSKRLGKNIIYSLNKKTIRPLVKAVN
                     QHTKDFCKNKCCKKEVKK"
BASE COUNT          540 a          320 c          240 g          711 t
ORIGIN      
        1 cagtattaac atctaagtaa tcaatatctt tagtttcaga taatatagaa tatagtataa
       61 tatctccttt atctagttgt tctgcatgat accaggccgg aattaacgtt ttcttatttt
      121 tagttcttaa atatttgtct cttttaggaa ctttaattgc ataaacaagg tgttcttttg
      181 ttaactttat ttctcctaat ctgtttttta atacaataat tgattcatta acacttctat
      241 caaatattcc agagatttta ttatattttc cattatggct aactattaaa tcttttgttt
      301 tgatgtcctt tatttttatt aatttagaat ttgtttgaag taatgtactt tcaactaagc
      361 acttagggtt tccaaccttt cccacaccat cagcattttt aatttctccc atattctttg
      421 ggttcataaa attttccata actttctttg aatagttttc cataattatt tcaccttata
      481 tttgtaagga ttcattttcc ttaatttatt tactgtatct ttaacagaat ctacaacaaa
      541 tttaatttcc gctttagtat tatcaattcc aacagaaaac cttacagttc cgtgtgtttg
      601 ttcttgcttt aagccaatag ccttcaagac atggcttggt tcaagagtat ttgaagaaca
      661 cgctgagcct gttgaaacag agataccaag agcatcaaga taaagtaaga ttgcttcgcc
      721 ttcagcatac ataaaactaa tgtttgcgtt attacataat cttttatttg aattaatact
      781 tggaccgttt agccaagaat cattaacgtc ctttaagcca ttaacaagtt gatttcttaa
      841 agtagtcata tgtgcaattt ctttttctcc aattacatca gtggctttag aaaaaccaat
      901 aattcctgga acattaactg tcccgcttct aagtttatat tcgtgtcctc caccatgtaa
      961 aagtggctca agttttattc cttctctaac aaataatccg ccaatgcctt ttggcccata
     1021 aattttatga gcattaattg ttaaaagatc aatattttct ttttcaaggt caatagggta
     1081 ttttgtaaag ctctggcagg catcaacatg taataatgag ccattatcat gaacaatctt
     1141 agaaatctct ttaatatttt gaagagtgcc aatctcgtta tttccatgaa ttacagaaac
     1201 aagaagagtt tcctttctaa gcgccttttc taagacgtca atctttacaa aaccctcttt
     1261 atcaacgtca agataagtaa ttgtataccc tttattttca agaaatttag ttacattaag
     1321 aacactgtca tgttcaatct ttgttgtaat tatgtgtttc ttttgtttat tgctctcagc
     1381 aaggccttta agcgcaaaat tattgctttc tgttccacca gaggtaaaaa tcagcttgtg
     1441 gttcttggaa ttaacttttt tagaaatctt ctctctagca atatctagtt tttctttagc
     1501 ctctcttcca acagaatgaa gactagaaga gttaccaaag tatttttctt ctgcttcaac
     1561 cacggctttc ttaacagaag aatgcattct tgtagttgct gcattatcaa gataaattga
     1621 cttcattttt tgacctcttt cttacagcat ttgtttttac agaagtcttt tgtgtgttga
     1681 ttaactgctt ttacaagtgg cctaatagtc ttcttattaa gagaatagat tatgtttttg
     1741 cccaatcttt tagaaaaaac aatattacaa tcaagtagta tctttaaagc atggctaact
     1801 ttagactgtt c
//
LOCUS       DAOOON010000060         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_87489_length_1742_cov_10.028453, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000060 DAOOON010000000
VERSION     DAOOON010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87489_length_1742_cov_10.028453"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PLK55_04425"
     CDS             complement(<1..1663)
                     /locus_tag="PLK55_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOZ36199.1"
                     /translation="MGDTLEFILVLIIIVVNGLIGFAQEYKADRAVDSLKKMLVKTTL
                     VLRDGQKQEINTEDLVPGDIVYVYEGDKVPADLRILENYSLVVDESVLNGESVPVSKD
                     LSSKDEGKNKLFSGTTIASGKGLAIVEKTGINTEFGKIINLVSKEEEIKTHLSKQIDK
                     LSKVLIYILIASFVILFVLGLLRGEGVLAIFLISVSLAISAIPEGLPIVMTLTLASGV
                     QLMSKKNAIVKKLSSIEALGATTVICSDKTGTLTLNEMNVEKVYTFNYEKKISSSGYL
                     SNQLEKIDNIDAHKLLDIANNCNNSEIDKQIFGDPTEIALKILAKKAQITNNYKFIDE
                     IPFSSQRKMMSTIHEISGSYEIFTKGAFGEVVNRCDRVLENGKVRKITDKDIKKFKEF
                     EEKYACQALRVLAFAYRPIINIKDKKEEKLILVGIVGMMDPPRPDIKEALGIARKAGI
                     VTKIITGDNPLTAKAIAEKIGFKNPKAVSANILDSLSDKEATELIYKTDIFARAKPEH
                     KYRIVELLQKAGEVVAVTGDGVNDAPALKKADIGIAMGIKGTEATK"
BASE COUNT          531 a          331 c          227 g          653 t
ORIGIN      
        1 cttttgtggc ctctgtgcct ttaattccca tagctatgcc aatatctgct ttctttaaag
       61 caggagcatc attgacccca tctccggtta cggctacaac ttctccggcc ttttgaagaa
      121 gctcaactat tctgtattta tgttctggtt ttgctcttgc aaagatgtct gtcttataga
      181 ttaattctgt tgcctcctta tctgataaag aatctaatat gtttgcagaa actgctttcg
      241 gatttttaaa accaatcttt tcagctatgg cttttgctgt taatgggttg tcaccagtta
      301 taatctttgt aacaattcct gcttttcttg caatacctag tgcttcttta atatctggtc
      361 ttggagggtc catcattcca acaataccaa ccaaaattaa tttttcttct tttttgtctt
      421 taatgtttat tattggccta taagcaaaag ctaatactct taaagcctgg caagcatact
      481 tctcttcaaa ctctttaaat ttcttaatgt ctttatctgt aatctttctt actttcccat
      541 tttctaaaac cctatcgcat ctatttacta cttcaccaaa tgcacctttt gtaaaaattt
      601 cataactccc actaatctcg tgaatagttg acatcatttt tctttgagaa gaaaatggta
      661 tctcatctat aaatttataa ttatttgtta tctgtgcttt ctttgctaga atctttaaag
      721 caatctctgt agggtctcca aagatttgct tatcaatttc tgaattatta cagttatttg
      781 caatatctag aagtttatgt gcatcgatat tatctatttt ttctaattga ttagatagat
      841 aaccagaact ggatattttt ttctcataat tgaaagtata aactttctca acattcattt
      901 catttaaagt taaggtgccg gttttatctg aacaaataac ggtggttgca ccaagtgctt
      961 ctattgaact taattttttt actattgcat tctttttaga cattaattgc acgccactag
     1021 caagagttaa agtcataact attggcaggc cttcagggat tgcagaaatt gcaagagata
     1081 cagaaattaa gaaaatagct aatacacctt ctcctcttaa caaccctaaa acaaaaagaa
     1141 ttacaaaact tgctatgaga atatatatta atacttttga aagcttatct atctgtttag
     1201 aaagatgtgt ttttatttct tcttcttttg agaccagatt aataatctta ccaaactctg
     1261 tatttattcc ggtcttttca acaattgcta ggcctttacc acttgctatt gtggtccctg
     1321 aaaataattt gtttttccct tcatctttac ttgaaagatc ttttgatacc ggaacgcttt
     1381 cgccgtttaa aactgactca tcgacaacta gagaataatt ttctagaatt cttaaatctg
     1441 cgggaacctt atccccctca tagacataaa caatatctcc aggcactaag tcttcagtat
     1501 taatttcttg cttttgccca tctcttagaa ctaatgttgt ttttactaac attttcttta
     1561 aagaatcaac tgctctgtct gctttgtatt cttgagcaaa accgattaga ccattaacta
     1621 cgataataat cagaactaaa ataaactcta aagtatcgcc cataaataaa aaaataaaag
     1681 aacatataaa aagaataata ataattaatg acgtaaactg agaaataatc atcttaagaa
     1741 ta
//
LOCUS       DAOOON010000061         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_110442_length_1510_cov_23.344330, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000061 DAOOON010000000
VERSION     DAOOON010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110442_length_1510_cov_23.344330"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..509)
                     /locus_tag="PLK55_04430"
     CDS             complement(102..509)
                     /locus_tag="PLK55_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36200.1"
                     /translation="MPNKPVKRKPSETRKFSKTLFKTKPKIELKEDTTNPHIKTINTM
                     FTSAKTVAEKITKVSFSANFFLRKKRSLEKVLNLIVNKTSPEKIFREFENFDIGEFRA
                     IGEKEYCSNLAKEINSLKQAYNYADNILRRLKK"
     gene            complement(521..>1510)
                     /locus_tag="PLK55_04435"
     CDS             complement(521..>1510)
                     /locus_tag="PLK55_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOZ36201.1"
                     /translation="QTVRADVPESEREKQKVNTHILKDLTNTAIKLEEYFGTPQIIEW
                     AMDKADIYIISSEAVNPNTAEKTKKSPQQKISSFKNQILLKGVGVNGGIVNGIIVTVI
                     NYNDLEKITHESILVTAMTDQEMMGAMKIARGIITNAGSSLCHAAVISKDLDKPCIVG
                     TRFATEQLLDGEKIQMNAKTGEIYAISSLDVVDIHPEEDDFLDEREQKIKQLWEEQRE
                     LREKGAVIINKKTPKKAITTPSHIIFNEPIEEKPKVVKEATPTSKYVDLQGNINIKDL
                     NHIEIEKVKQIVIPINLLFGPDDITKLKGEFSGSVCMVAKEKIKKILKDIKDF"
BASE COUNT          394 a          252 c          223 g          641 t
ORIGIN      
        1 atatttgttc ttaattatat aaagaataag cgccttgatg gcagaccggt tatgcattcg
       61 cctgcagagc gaaagaacag agttcgactc tctgtcaagg cttatttctt tagtcttcta
      121 agaatattat ctgcgtaatt atatgcttgt tttaaagagt ttatttcttt tgctaaatta
      181 ctacagtatt ctttttcgcc aattgcacga aattcgccga tatcaaaatt ctcaaattct
      241 ctaaatattt tttcaggact tgttttattt acaatcaaat ttaacacttt ttctaaactt
      301 ctttttttcc ttaaaaaaaa gttcgcagaa aaactcactt ttgttatttt ttcagcaacg
      361 gttttggcac tagtaaacat tgtgtttatt gttttaatat gcgggtttgt ggtgtcttct
      421 tttagttcta tttttggttt agttttaaat aatgttttac taaattttct agtttcgctg
      481 ggttttcgtt ttactggttt atttggcatt tttaatcctt ttagaaatcc tttatgtctt
      541 ttaaaatttt tttaattttt tctttagcaa ccatacaaac agagccagag aattctcctt
      601 taagttttgt tatatcgtct ggtccgaaca ataagttaat tggaataaca atctgcttga
      661 ctttttcaat ctcaatatga tttaaatctt taatattaat attgccttgc aagtccacat
      721 attttgaagt gggtgtagct tctttaacca cttttggttt ttcttcaatt ggttcattaa
      781 agattatgtg gctcggtgta gtgattgctt tctttggagt ttttttatta ataattactg
      841 ctcctttttc tctaagttct ctttgttctt cccaaagctg tttaattttt tgttctcttt
      901 catctagaaa atcgtcttct tcaggatgaa tatcaacaac atcaagagaa gaaatagcat
      961 atatttctcc agtttttgca ttcatttgta tcttctcgcc atcaagaagc tgttctgttg
     1021 caaatcttgt gccaacaata catggtttat caagatcttt agagattact gctgcatggc
     1081 ataaagagct tccagcatta gtaattatcc ctctagcaat cttcatagcc cccatcatct
     1141 cttggtcagt cattgctgtt actagtattg attcgtgtgt aattttttca agatcattat
     1201 agttaattac tgtaacaata attccattaa caattccacc attaactcca acgcccttta
     1261 ataatatttg atttttaaaa gagctaatct tttgttgagg gctttttttt gttttttctg
     1321 cggtgtttgg gtttactgct tctgaagata taatataaat atcggcttta tccatagccc
     1381 attcaataat ctgtggagtt ccgaaatatt cttctagttt tattgcggtg tttgttaagt
     1441 cttttagaat atgagtatta actttttgtt tctctctttc tgattcaggc acgtcagctc
     1501 taacagtttg
//
LOCUS       DAOOON010000062         1490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_112952_length_1490_cov_26.381185, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000062 DAOOON010000000
VERSION     DAOOON010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112952_length_1490_cov_26.381185"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLK55_04440"
     CDS             <1..570
                     /locus_tag="PLK55_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOZ36202.1"
                     /translation="RDIFKKARQLAPSIVFIDEIDSITSGRTGLETNDAKNNVIAQLL
                     TEIDGVSNLKDIVIIGATNRPDLIDPAFLRPGRFEKLLLVPLPEESTREKIFDVYLGK
                     MNVDKKASSKEFAKLTDGFSGAEIEAVCREAGLTKIRLIIEDLEKDKKLDNPKPIVTK
                     DAFIKAIEKFKAKKENPEKEEKDKDYAIR"
     gene            complement(588..1250)
                     /locus_tag="PLK55_04445"
     CDS             complement(588..1250)
                     /locus_tag="PLK55_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36203.1"
                     /translation="MAKKEKKSLIIWAVAALVLGVLIGVLITNSVTGKAKASISEKEN
                     LENSQIKGLPSSSGKCCPAAGGGSCNIGEVCSYSTIQINDYGDAYSVNAECTCRKIEN
                     TPNIRGADLVAGNKIPLTNDIIQKAIKTQLADGKTKYDFIVSENGDIIAPGTRDIAGD
                     GCGCHTASSCPCGSICVSGSTSSTESDDGYEIVTINQISTCRQNYSGTSGYTSGETEY
                     SH"
BASE COUNT          494 a          252 c          236 g          508 t
ORIGIN      
        1 agagatatct ttaagaaagc tagacaactt gcgccaagca ttgtttttat tgacgaaatt
       61 gattccataa cttcaggaag aactggcctt gaaacaaacg atgcaaagaa taatgttatt
      121 gctcagttgc taactgaaat tgatggggtt tctaatctta aagacattgt tattattgga
      181 gcaaccaatc gcccagactt gattgatcca gcatttcttc gcccaggaag atttgaaaaa
      241 ctgcttttag ttcctttacc agaagaatca acaagagaaa aaatatttga cgtatactta
      301 ggaaaaatga atgtagataa aaaagcaagc tcaaaagaat ttgcaaaact tacagacggg
      361 ttttctggtg cagagattga agcagtctgt agagaagcag gactaacaaa gattagacta
      421 attattgaag acttagaaaa agacaaaaaa cttgataacc ctaaaccaat tgtaacaaaa
      481 gacgcattta ttaaagccat agaaaagttt aaggcaaaga aagaaaatcc agaaaaagaa
      541 gaaaaagata aagattatgc aattagataa tcttttttaa ttttaactta atggctatat
      601 tcagtttctc cagaagtata ccctgatgtc ccagaataat tctgtctaca tgtgcttatt
      661 tgattaatag tcacaatttc ataaccgtca tcagattctg tagatgatgt gcttccagaa
      721 acacaaatac tcccacacgg acaagaacta gccgtgtgac acccgcaacc atcgccagct
      781 atatctcgtg ttcctggcgc gatgatatct ccattttcac taacaataaa atcatatttg
      841 gttttcccgt cggctaattg ggtttttatt gccttttgta ttatatcgtt agtcaatggt
      901 attttattgc cagcaactaa atcggcgcct cttatatttg gcgtgttctc tatttttcta
      961 caagtacatt ctgcatttac gctatatgca tcgccataat cattaatttg aattgttgaa
     1021 tatgaacaga cttcgcctat attacaggag ccccctcctg ctgcaggaca acacttgcca
     1081 cttgaactgg gtagtccttt tatttgagaa ttttctaaat tttctttttc agagatactg
     1141 gcttttgctt ttcctgttac tgaatttgtt attaatacgc caattaatac tcctagcact
     1201 agagccgcta ctgcccaaat gattagtgat tttttttctt tttttgccat tttaatttcc
     1261 tccattttta aaaagtttaa tatgattaaa agaaggaata accgatatat atatatatat
     1321 atattgtgtt ttaataaaaa atcaaaatct tttgtttatt gagtgttata aatctttaaa
     1381 agaaattaaa ttagatcttt tcttagaatc cgtccgtctt ttgtttagtt tttttaaaaa
     1441 ggcttttgtt gcctttatat ttttttaata ttatatagga tagaataatc
//
LOCUS       DAOOON010000063         1472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_115209_length_1472_cov_16.428370, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000063 DAOOON010000000
VERSION     DAOOON010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115209_length_1472_cov_16.428370"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..894)
                     /locus_tag="PLK55_04450"
     CDS             complement(79..894)
                     /locus_tag="PLK55_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36204.1"
                     /translation="MSPKLISSKKGRLINNSFVNSKKTIPEKRVVISKSKINFLETDP
                     KKLNFQLYKSNPLRLRAFFTAQGITKENFNSSFLEKIIENNKRNYKLEKEKYDNWFKT
                     RKTAVAHTEDYHPKIRIDIKTFLNIGDSLRLSTREIINAHSRYVSSYDPRIRASQKVY
                     SFLISLDAKEQNRRPDLLKSIIQEHGIKEGFFGLSEYRRTIKLLQVREETKQHNLANI
                     PENLVDKITLETLFEIENMGFTAKEIIRELKPYSGVYGSIASKAITEYIKRKK"
     gene            complement(913..>1472)
                     /locus_tag="PLK55_04455"
     CDS             complement(913..>1472)
                     /locus_tag="PLK55_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOZ36205.1"
                     /translation="IKDLQDISEKEPLLNSTIEEEVKKRELITLYKKMNVLIENLSTE
                     ISDYEKNIAKGDVFLENFRCYRTISFEGPKKSREFLEKLVDSFEIQEFVLKFNVVGTV
                     DLNNIAEHIQGRKVGIDIIVPATNIQLIFDEILKAQTLKFRLSSESLVIYFEKDTVLH
                     IEGPSKKIKIADTIAKDFDAKLINE"
BASE COUNT          441 a          224 c          175 g          632 t
ORIGIN      
        1 aaaaagtaat gctaataaaa gttagaccca gaattacttg ggatggtgga aagactgcca
       61 atgtttttat tgattaaatt atttttttct ttttatatat tctgttattg ctttagaagc
      121 tatacttcca taaactccag aatatggttt taattctcta attatttctt ttgctgtaaa
      181 acccatattt tcaatttcaa ataaagtttc aagcgtaatt ttatctacta aattttctgg
      241 aatatttgcc aaattgtgct gttttgtttc ttctcttact tgtaatagtt ttattgtgcg
      301 cctatattca cttaatccaa aaaaaccttc ttttatgccg tgctcttgaa ttattgattt
      361 tagtaaatct ggtcttctgt tttgttcttt agcatctaaa gaaattaaaa aagagtatac
      421 tttttgacta gcacgaattc ttgggtcata acttgaaaca tatctgctat gagcattaat
      481 aatttctcta gtacttaatc ttaaagaatc gccaatattt aaaaacgttt ttatatctat
      541 tctaattttt ggatgatagt cttctgtatg tgcaactgct gtttttctag ttttaaacca
      601 gttatcatat ttttcttttt ctaatttata gtttctttta ttattttcaa taattttttc
      661 taaaaaagaa gaattaaagt tttctttagt tatgccttgt gcagtaaaaa aagcccttaa
      721 tcttaaagga tttgatttat ataattgaaa attaagtttt tttggatcag tctccaaaaa
      781 attaatcttg gatttagata taacaactct tttttcggga atcgtttttt tagaatttac
      841 aaaagaatta ttaatcaatc ttcctttttt agacgaaatt aattttggtg acattttttt
      901 ccccttttta aattattcat taatcaattt cgcatcaaag tctttagcaa ttgtgtcagc
      961 aatcttaatc ttcttgcttg gaccttctat atgaagcact gtgtctttct caaaatagat
     1021 tactaggctt tctgaggata atctaaactt taaagtctgt gctttaagta tctcatcaaa
     1081 aatcaattga atatttgttg ctggaacaat aatgtctatt cctacttttc taccttgtat
     1141 gtgctctgca atattattta gatcaactgt tcctacaaca ttaaacttta aaacaaattc
     1201 ctgtatttca aaagaatcaa caagcttttc taaaaactct ctggatttct ttgggccttc
     1261 aaaacttatg gttctgtagc atctgaaatt ctctagaaaa acatcacctt ttgctatatt
     1321 tttctcataa tctgaaatct cggtacttag gttctctatt aatacattca tttttttata
     1381 taaagttatt aactctcttt tctttacttc ttcttctatt gttgaattta atagtggctc
     1441 tttttcacta atatcttgta agtctttaat gt
//
LOCUS       DAOOON010000064         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_116846_length_1459_cov_11.542735, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000064 DAOOON010000000
VERSION     DAOOON010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116846_length_1459_cov_11.542735"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..615)
                     /locus_tag="PLK55_04460"
     CDS             complement(58..615)
                     /locus_tag="PLK55_04460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOZ36206.1"
                     /translation="MNNIYFFMRHGQSLANLKHLVITKPENAIDHYGLSAIGKKQVLN
                     SISKNKDLNKDTIIYSSDFKRAKETAEIVREHIKAKPIIFSKNLRERALGVYEKGKDK
                     MYRELWENDLKENTLKSCESISSIAKRFKELITDIEKKHKGKKILFVTHGDIISVGLS
                     VLNNKPVKEHHKRYSILENAEIKKS"
     gene            complement(612..>1459)
                     /gene="mnmA"
                     /locus_tag="PLK55_04465"
     CDS             complement(612..>1459)
                     /gene="mnmA"
                     /locus_tag="PLK55_04465"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOZ36207.1"
                     /translation="LGIEYHVVDERKNFEKEVVSYFKSELKKNKTPNPCVICNRYSKL
                     KALLDFADNHNIKYVATGHYAKIKNRNLCKPKDKTKDQTYGLCLLPREWLKRIIFPLE
                     GMTKEQVYKLAQKQDFDFFSKTKQSQDFCYVSGSDISKFVEKELGKKPGKIIDTTGKV
                     LGTHNGLWFYTLGQRKGLGRDGKRYFVCGFDIKKNYLIITDKQEKKIKEISLEKVNLI
                     TKKLPKNLKVKIRYTLTEISAELILQKGNKAKVIFKQPQAVVTPGQFCVFYSKDKCLG
                     GGIIK"
BASE COUNT          392 a          255 c          220 g          592 t
ORIGIN      
        1 cttattccat agagaatact atatattaag aatcaaaggc tttttaagtg tgtggtatta
       61 acttttctta atctctgcat tttctagaat tgaatatctt ttgtgatgct cttttactgg
      121 cttattgttt aaaaccgaaa ggccaacgct aataatgtct ccgtgagtta caaacaaaat
      181 cttctttcct ttgtgtttct tttcaatatc tgtaattaat tctttaaatc ttttagcaat
      241 tgatgaaatg ctctcgcagc tttttagagt attctctttt aggtcatttt cccaaagctc
      301 gcgatacatt ttgtctttac ctttctcata cacgccaaga gctctttccc ttaggttttt
      361 tgaaaaaatt attggctttg ctttgatgtg ttctcttaca atctctgcag tttcttttgc
      421 tcttttaaaa tctgaagaat agataattgt gtctttattt aaatctttat tcttggaaat
      481 agaatttagc acttgctttt taccaattgc tgaaaggccg tagtgatcaa ttgcattttc
      541 tggttttgta attacaagat gctttaagtt agcaaggctt tgaccatgcc tcatgaaaaa
      601 ataaatatta ttcattttat tattcctccg ccaaggcatt tgtctttaga atagaaaaca
      661 cagaactgac caggagtaac aactgcttgt ggttgcttga agattacttt cgctttgttg
      721 cctttttgta aaatcaattc tgcggaaatc tctgttagcg tatacctaat ctttactttt
      781 aagttttttg gtaatttctt tgttataaga tttactttct ctaaagatat ctctttgatt
      841 tttttttctt gcttgtctgt tattattaaa taattttttt taatatcaaa gccgcaaaca
      901 aaatatctct tgccatctct cccaagacct tttctttgac caagggtgta gaaccataaa
      961 ccattatggg taccgagcac ttttcctgtg gtgtcaatta tcttacccgg tttctttcca
     1021 agctcttttt ctacgaactt ggaaatgtcg ctaccagaaa cataacagaa atcctgagac
     1081 tgtttggtct ttgagaaaaa atcaaagtcc tgtttttgtg ccaacttgta aacttgctct
     1141 tttgtcatgc cttctaaagg gaaaataatt ctttttagcc actctctcgg cagaagacaa
     1201 aggccataag tttgatcttt tgttttgtct tttggcttac aaagatttct gttttttatt
     1261 tttgcgtagt ggccagttgc aacatatttg atgttatgat tgtctgcaaa atctagaaga
     1321 gctttaagtt ttgaatatct attacaaatc acgcaagggt ttggtgtttt attttttttt
     1381 aattcagatt taaaataaga aaccacttct ttttcaaagt tttttctttc atcaaccacg
     1441 tgatattcaa ttccgagct
//
LOCUS       DAOOON010000065         1150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_173731_length_1150_cov_12.278539, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000065 DAOOON010000000
VERSION     DAOOON010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173731_length_1150_cov_12.278539"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..331)
                     /locus_tag="PLK55_04470"
                     /pseudo
     CDS             complement(1..331)
                     /locus_tag="PLK55_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
     gene            complement(557..760)
                     /locus_tag="PLK55_04475"
                     /pseudo
     CDS             complement(557..760)
                     /locus_tag="PLK55_04475"
                     /inference="COORDINATES: protein motif:HMM:NF025881.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
BASE COUNT          341 a          181 c          157 g          471 t
ORIGIN      
        1 tacatgcatt tgtttttatt ttttttgttt tctttccatg tttagaataa tagtcattaa
       61 ttgctgcttg aagcccatct gctgcaaggt ttgagcaatg catcttaact ggcggtaagc
      121 ccccaaggtt ttctgcaaca tctgctcttg tgatattaaa ggctttatca agagtctttc
      181 ctttagcaag ctctgtgacc atagaagatg ttgcaattgc tgctgcacac ccaagtgttt
      241 taaactttat atctttaatt atttcttttc ctgctttgtc ttttgaaacc ttaatgtaaa
      301 tccacattat atctccgcaa ttatgaacag taaatgcttc agtattataa gagtgagatg
      361 tgtttatttc taaattataa actttcccgg aatagttttc tgttttttta gaagtgacag
      421 gtgtatataa tacatttttg tctatccaag attttattgc ttctggtctt agattataga
      481 aagtaacgcc aatgatttta gacagttttg ttgtagattc tttgtctcct acatgtattc
      541 tataacattc ctgatgtttt agcccgtttt tgtcaatttt atctgtttca taatacaccg
      601 aaggaataat cccttgtctt agtaataata tttttagctt gtggactaat tctttagata
      661 tagttgaata tcctcctcta gggattcttg tgttaaaaaa accatctcct ttccaaattc
      721 cataaattag aggtttttgt ttttcaggag gtaatgtcat aaaaatttca ggtatatctt
      781 tatttttagc acctgattca aataatattt taaataatct aaccacaaat acactattta
      841 tgtaaactac ttgagaatgt ttagattcat tctttcttac agtcggagtt attccaaata
      901 ttttttcaga aattctaata atatcttcaa caagattatc ttcctttaaa ttaaaacaaa
      961 acgcgatgtg tgtttttgtg actttatcag aataatatcc ttctgaaaga taataacccg
     1021 ctaatcttaa aaaatcagaa tctaatttta tttttgaagg aatttctttg cttctataat
     1081 cgaatttttt tttttcagta ttaacatcta agtaatcaat atctttagtt tcagataata
     1141 tagaatatag
//
LOCUS       DAOOON010000066         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_183764_length_1113_cov_11.300567, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000066 DAOOON010000000
VERSION     DAOOON010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183764_length_1113_cov_11.300567"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..697)
                     /locus_tag="PLK55_04480"
     CDS             complement(59..697)
                     /locus_tag="PLK55_04480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOZ36208.1"
                     /translation="MKKLEGVVIRLSVGIGKNVLEAEEALRIAKRKKYQKKEYNIEMY
                     SGKSIPNNTDTPNKVISAYLRLEKKLSNLKNSLKDLEMLKIDHKTGLLNRFGYFVERD
                     KLIKQKKYGKNRFIIFFDADSMHSLNNKYGYAFVDRYLTAIGKALNENIRNRDQKDIL
                     NVGESLNNRRNDTAGDEFIIDISCKEKDVLNITKRYLTKCYEYQRKIKIKKT"
     gene            complement(704..>1113)
                     /locus_tag="PLK55_04485"
     CDS             complement(704..>1113)
                     /locus_tag="PLK55_04485"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOZ36209.1"
                     /translation="VCLDIQKMIKKEFKKNVEVMVYGDGAYKDPESGIYELADPVCAF
                     GVTPGIESVRRVGVKTKYLMQKFLSEGKSKEEILKAIEKEKKRIMCGTDKGEIACEGT
                     TPRKVRNLVASLADLVSGSADANTPVVIVRGFI"
BASE COUNT          318 a          200 c          131 g          464 t
ORIGIN      
        1 gcaataactg gagtaattgc agctcaagga attatagaaa aagaaaagca aaaatctctt
       61 aagtcttttt aatttttatt tttctttgat attcatagca ttttgtaaga tatcttttag
      121 taatatttaa aacatccttt tctttacatg aaatatcaat aataaattcg tctcctgcag
      181 tatcgtttct tctattgttt agtgattctc caacatttag aatatctttt tgatctctat
      241 ttcttatatt ctcatttaag gctttaccaa ttgcagttaa atatctgtct acaaaggcat
      301 agccatattt attatttaga gaatgcatgg aatctgcatc aaaaaaaata ataaatctgt
      361 ttttcccata ttttttttgt ttaatcaatt tatctctttc aacaaaataa ccaaatctgt
      421 ttagtaatcc tgttttatga tcaattttta gcatctcaag atctttaagt gaattcttta
      481 agttagataa ttttttttct aatcttaaat aagcagaaat tactttgttt ggtgtgtctg
      541 tgttattagg aattgatttt ccagaataca tctcgatatt atattctttc ttttgatatt
      601 tttttctctt tgcaattctt aatgcttctt ctgcttcaag gacattcttg ccaattccta
      661 cacttaaacg tattacaacg ccttctaatt ttttcataat tatttagata aaccctctaa
      721 caataacaac aggtgtgttt gcatcagcac ttcctgaaac aagatcagca aggcttgcta
      781 caagatttct aactttcctt ggagttgtgc cttcgcaagc gatttctcct ttatctgtgc
      841 cacacattat tctttttttt tctttttcaa ttgcttttaa tatttcttct ttgcttttgc
      901 cttcacttaa aaatttctgc attagatatt tggtttttac gcccactctt cttacgcttt
      961 caattcctgg ggttacgcca aaagcacaga caggatctgc aagttcatag attcctgatt
     1021 ctggatcctt gtaagcacca tcgccataga ccatgacttc tacatttttc ttaaattctt
     1081 ttttaatcat tttttgtata tcaagacaaa cct
//
LOCUS       DAOOON010000067         1106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: archaeon isolate AMR_MDS_2622
            NODE_185793_length_1106_cov_21.001903, whole genome shotgun
            sequence.
ACCESSION   DAOOON010000067 DAOOON010000000
VERSION     DAOOON010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864697
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      archaeon (wastewater metagenome)
  ORGANISM  archaeon
            Archaea.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:23:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 898
            CDSs (total)                      :: 862
            Genes (coding)                    :: 857
            CDSs (with protein)               :: 857
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185793_length_1106_cov_21.001903"
                     /isolate="AMR_MDS_2622"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1906665"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLK55_04490"
     CDS             <1..861
                     /locus_tag="PLK55_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOZ36210.1"
                     /translation="CGWRYKNDIPSESKFSRVFKELSELKIAQKTHEQFVKEYLRDTL
                     FFYNASDATKIPLREKPVKVEKEKFKPKRRGRPKKGETREPKTPSILQQQEDMKTTKQ
                     MLSLVSTQCGVGRKQNSKGNFETWIGGKLHISVVDGDIPITAIYSGANVHDSSVALPL
                     INETSKKVSYLYDLQDAGYDSNIIRDFSKKLNHRPLIDINPKNSKELKGKIQLIKDEK
                     KKFEILNLTQSLDTHHYNQRSMVERVNKYLKEDFGCSNIYYKGATKVASVLAFGILSV
                     CIHQSLKMVT"
BASE COUNT          441 a          162 c          195 g          308 t
ORIGIN      
        1 tgtgggtgga gatataaaaa cgatattcca agtgagtcta aatttagtag agtctttaag
       61 gagctcagtg agcttaaaat tgcacaaaag acgcatgagc agtttgtgaa ggagtatcta
      121 agggatacac tcttttttta taatgcaagt gatgcaacaa aaataccact gagagaaaaa
      181 cctgtaaaag ttgaaaaaga aaagtttaaa ccaaaaagaa gaggacgacc taaaaaaggt
      241 gaaacaagag agcctaaaac acccagtatc ttgcagcaac aagaagatat gaaaaccaca
      301 aagcagatgc tatctttggt atcaacgcag tgtggagttg gaagaaaaca gaattccaaa
      361 ggcaactttg aaacatggat aggagggaaa ctccatatca gtgtagtaga tggagatatc
      421 cctattactg ctatttattc aggggcaaat gttcatgata gctcagtagc acttcctctt
      481 ataaacgaga caagcaaaaa agtatcatat ctttatgact tacaagatgc aggatacgac
      541 agcaatatta tcagagattt ttccaaaaaa ctaaatcata gaccgcttat tgatatcaat
      601 ccgaagaact ccaaagagtt aaaaggaaaa attcaactta taaaagatga aaaaaagaaa
      661 tttgaaattc taaaccttac tcaaagttta gatacccatc actataacca aagaagtatg
      721 gttgagcgtg taaataaata cctcaaagaa gactttggat gcagtaatat ttattataaa
      781 ggagctacaa aagtagcttc tgttttagca tttggtattt tatcagtttg tattcatcag
      841 agtttgaaaa tggtaacata acttttagct aaaaacacta aaaattagcc atttttaaaa
      901 ctcaaccatc aagcacgcac ccaataatag ttttttatct aaaattgacc cataaagcgg
      961 taaaatgatt aaaagtttaa aagatctgct tgataagttt agtaggttta caaattcact
     1021 agtttatgat ggtggaattt gcaagcggct ctaaaaaata atagttatat tgtagatgat
     1081 tttaatttat tctctaaata atttaa
//
